TWI866690B - Biological sample testing device - Google Patents

Abstract

A biological sample detection device comprises a glass slide, a spacer layer and a cover glass. The glass slide comprises a top surface, the spacer layer is arranged on the top surface, and comprises an inlet, a chamber and an exhaust port, the exhaust port is arranged opposite to the inlet along a spreading direction, the chamber has a front section, a middle section and a rear section, the front section is defined by a pair of first guide surfaces, a first angle is formed between the two first guide sections, the rear section is defined by a pair of second guide surfaces, a second angle is formed between the two second guide sections, the second angle is greater than the first angle, and the second angle is between 140 degrees and 180 degrees. The cover glass is arranged on the spacer layer and closes the chamber. The design of the chamber can prevent bubbles from being generated on both sides of the chamber when a biological sample is injected.

Description

Biological sample testing device

The present invention relates to a medical testing instrument, and more particularly to a biological sample testing device.

Referring to FIG. 1 , there is a sperm detection device (US patent USD0566849S), which comprises a glass slide 2, a spacer 3 and a cover glass 4. The spacer 3 has a plurality of ribs 301, and two counting chambers 5 are defined by the ribs 301. Each counting chamber 5 has an inlet 501, a chamber 502 connected to the inlet 501, and an exhaust port 503 connected to the chamber 502. The chamber 502 has a front section 504 connected to the inlet 501 and located at the inner side of the inlet 501, a middle section 505 connected to the front section 504, and a rear section 506 between the middle section 505 and the exhaust port 503. The front section 504 is defined by a pair of first inclined surfaces 504', the middle section 505 is defined by a pair of straight surfaces 505', and the rear section 506 is defined by a pair of second inclined surfaces 506'. Each straight surface 505' is connected to the corresponding first inclined surface 504' and second inclined surface 506' at an angle.

When the biological sample drips from the inlet 501, the biological sample will automatically spread and flow toward the exhaust port 503 through capillary action. The biological sample located on both sides of the chamber 502 and spread along the first inclined surface 504', the second inclined surface 506' and the straight surface 505' will spread and flow faster than the biological sample located in the middle of the chamber 502 due to the wetting effect. However, because 1. the spreading path of the biological sample on both sides of the first inclined surface 504', the second inclined surface 506' and the straight surface 505' is longer than the spreading path of the biological sample in the middle 2. each straight surface 505' is connected to the corresponding first inclined surface 504' and the second inclined surface 506' at an angle and produces a diamond-shaped turning structure, the biological sample on both sides of the first inclined surface 504', the second inclined surface 506' and the straight surface 505' will turn and stop. Therefore, the biological sample in the middle, which originally spreads slower, will eventually spread ahead of the biological sample on both sides of the first inclined surface 504', the second inclined surface 506' and the straight surface 505'. This results in the formation of vacuum bubbles 6 near the exhaust port 503 on each second inclined surface 506', which in turn causes detection errors. The first point above is based on the basic function of the detection device and does not need to be overcome (the chamber must have a transverse axial space perpendicular to the spreading direction, so the spreading path of the biological sample along the inclined surfaces and the flat surface on both sides must be longer than the spreading path in the middle), so the present invention is to overcome the problem of the second point above.

Therefore, the purpose of the present invention is to provide a biological sample detection device that can solve the existing deficiencies.

Therefore, the biological sample detection device of the present invention includes a glass slide, a spacer layer and a cover glass. The glass slide includes a top surface. The spacer layer is arranged on the top surface, and includes an inlet, a chamber connected to the inlet, and an exhaust port connected to the chamber. The exhaust port is arranged opposite to the inlet along a spreading direction. The chamber has a front section connected to the inlet and located on the inner side of the inlet along the spreading direction, a middle section connected to the front section and located on the inner side of the front section along the spreading direction, and a rear section between the middle section and the exhaust port. The front section is defined by a pair of first guide surfaces. The first guide surfaces are opposite to each other and spaced along a transverse axis perpendicular to the spreading direction. Each first guide surface is provided with a spaced relationship between the inlet and the chamber. The surface has a first concave arc and a first guide section extending from the first concave arc toward the suction port, the first guide sections are in a gradually expanding inclined plane shape, and a first angle is formed between the first guide sections. The rear section is defined by a pair of second guide surfaces, the second guide surfaces are opposite and spaced along the transverse axis, each second guide surface has a second concave arc and a second guide section extending from the second concave arc toward the exhaust port, a second angle is formed between the second guide sections, the second angle is greater than the first angle, and the second angle is between 150 degrees and 180 degrees. The cover glass is spaced from the slide glass and is disposed on the spacer layer, and closes the chamber.

The effect of the present invention is that: by utilizing the design that each first guide surface has the first concave arc and the first guide section extending from the first concave arc toward the suction port, it is possible to prevent the biological sample from turning and stopping at the angle connection point between the two sides of the chamber, so that when the biological sample spreads smoothly to the two sides of the chamber, it is possible to prevent the biological sample in the middle from overflowing from the exhaust port due to spreading ahead of the two sides, and to prevent the formation of vacuum (bubbles) near the exhaust port on each second inclined surface. Furthermore, by utilizing the cooperation of each second guide surface having the second concave arc and the second guide section extending from the second concave arc toward the exhaust port, the biological samples on both sides are effectively drawn to the middle in advance, so that the original flow rate of the biological samples is lower than the middle flow rate of the two sides, so that the biological samples in the middle can be buffered when the biological samples on both sides converge, avoiding the risk of biological sample cell DNA being broken due to the collision between the biological samples on both sides and the biological samples in the middle, or the formation of vacuum bubbles on both sides of the chamber due to the biological samples in the middle flowing to the exhaust port earlier than the biological samples on both sides.

2 to 4 , an embodiment of the biological sample detection device of the present invention includes a slide glass 10 , a spacer layer 20 , and a cover glass 30 .

The slide glass 10 is made of float glass and includes a top surface 11.

The spacer layer 20 can be made of super-hydrophobic resin material, such as fluorine-containing polyester, polyurethane or acrylic resin, etc., and can be used alone or in combination. The super-hydrophobic resin material is coated on the top surface 11 of the glass slide 10 by screen printing and is cured at high temperature. In this embodiment, the spacer layer 20 includes two wells 201 (well). Each well 201 has an inlet 21, a chamber 22 connected to the inlet 21, and an exhaust port 23 connected to the chamber 22. The exhaust port 23 is arranged opposite to the inlet 21 along a spreading direction X. The depth of each well 201 is 10um or 20um.

The suction port 21 is defined by a pair of leading faces 211 and a first end face 212 intersecting and connecting the leading faces 211. Each leading face 211 has a first inclined surface section 213 and a first guide arc 214 connected to the first inclined surface section 213. The first end face 212 is in an arc shape, and the first inclined surface sections 213 gradually move away from each other when extending from the first end face 212 toward the corresponding first guide arc 214, which can reduce the curvature of the first guide arc 214 and reduce the curvature. A curvature radius R of each first guide arc 214 is 1 mm.

The chamber 22 has a front section 221 connected to the suction port 21 and located inside the suction port 21 along the spreading direction X, a middle section 222 connected to the front section 221 and located inside the front section 221 along the spreading direction X, and a rear section 223 between the middle section 222 and the exhaust port 23.

The front section 221 is defined by a pair of first guide surfaces 224, which are opposite and spaced along a transverse axis Y perpendicular to the spreading direction X. Each first guide surface 224 has a first concave arc 225 and a first guide section 226 extending from the first concave arc 225 toward the suction port 21, and the first guide section 226 is connected between the first concave arc 225 and the corresponding first guide arc 214, that is, each first guide arc 214 is connected between the corresponding first inclined surface section 213 and the corresponding first guide surface 224. A radius of curvature R1 of each first concave arc 225 is 6 mm. And a first angle θ1 is formed between the first guide sections 226. The first angle θ1 is between 90 and 110 degrees. In this embodiment, the first angle θ1 is 99 degrees. Although the first angle θ1 is as small as possible (to avoid increasing the amplitude of the flow turning of the biological samples 1 on both sides and increasing the flow path on both sides, and being exceeded by the biological sample 1 in the middle), it still needs to be able to take out the transverse Y space of the chamber 22.

The middle section 222 is defined by a pair of inner side surfaces 227 , and the inner side surfaces 227 are substantially parallel to the spreading direction X.

The rear section 223 is defined by a pair of second guide surfaces 228, which are opposite and spaced apart along the transverse axis Y. Each second guide surface 228 has a second concave arc 229 and a second guide segment 220 extending from the second concave arc 229 toward the exhaust port 23, and the second guide segments 220 are respectively disposed on both sides of the exhaust port 23 along the transverse axis Y, and the second guide segments 220 are substantially orthogonal to the spreading direction X. A curvature radius R2 of each second concave arc 229 is 5 mm, and a curvature radius R1 of a first concave arc 225 of each first guide surface 224 is greater than a curvature radius R2 of a second concave arc 229 of each second guide surface 228. A second angle θ2 is formed between the second guide sections 220. The second angle θ2 is greater than the first angle θ1 and is between 140 and 180 degrees. In this embodiment, the second angle θ2 is substantially 180 degrees. The larger the second angle θ2, the better (so that the biological samples 1 on both sides can better enclose the biological sample 1 in the middle), but it cannot exceed 180 degrees to cause the biological samples 1 on both sides to flow back.

The exhaust port 23 is defined by a pair of rear guide surfaces 231 and a terminal end 232 connected to the outside of the recessed portion 201. Each rear guide surface 231 has a second inclined surface segment 233 and a second guide arc 234 connected between the second inclined surface segment 233 and the corresponding second guide segment 220. The second inclined surface segments 233 gradually move away from each other when extending from the terminal end 232 toward the corresponding second guide arcs 234, which can slow down the exhaust speed and provide a buffer for the biological sample 1 in the middle. Each second guide arc 234 is connected between the corresponding second inclined surface segment 233 and the corresponding second guide segment 220.

The cover glass 30 is made of float glass and is spaced apart from the glass slide 10. The cover glass 30 is placed on the spacer layer 20 and seals the chamber 22. In actual implementation, the cover glass 30 is bonded to the spacer layer 20 through a glue layer (which may be a highly transparent UV glue, not shown), or a plurality of glue cavities 202 (see FIG. 3 ) for the glue to be embedded are further opened in the spacer layer 20, and the glue layer is covered on the surface of the spacer layer 20 and the glass slide 10 (i.e., in the glue cavities 202) by screen printing or dispensing. Thus, the cover glass 30 can be directly bonded to the slide glass 10 in addition to being bonded to the spacer layer 20, which can improve the firmness and prevent cross contamination.

In order to further understand the effects of the cooperation of the various components of the present invention, the technical means used, and the expected effects to be achieved, the following description will be given. It is believed that this will provide a deeper and more specific understanding of the present invention.

2, 4 and 5, when the cover glass 30 is placed on the spacer layer 20 and the chamber 22 is closed, most of the suction port 21 and part of the exhaust port 23 are not shielded by the cover glass 30. The operator can inject the liquid biological sample 1 from the suction port 21, and as shown in FIG6, the biological sample 1 gradually spreads along the spreading direction X from the suction port 21 to the exhaust port 23 through the capillary action.

As shown in the schematic diagram of each stage of Figure 6:

As shown in the schematic diagram (a) of FIG. 6 and in conjunction with FIG. 4 , after the biological sample 1 is injected from the suction port 21, the first inclined surface sections 213 of the suction port 21 are inclined and gradually move away from each other when extending from the first end surface 212 toward the corresponding first guide arcs 214. In addition to allowing the biological sample 1 to spread in the spreading direction X, the biological sample 1 can also be guided to spread toward the first guide surface 224.

As shown in the schematic diagram (b) of FIG. 5 and in conjunction with FIG. 3 , when the biological sample 1 enters the front section 221 of the chamber 22, the first guide sections 226 of the first guide surfaces 224 are in the shape of gradually expanding slopes, so that the biological sample 1 not only spreads in the spreading direction X space of the chamber 22, but also spreads in the transverse direction Y space of the chamber 22 along the first guide sections 226. However, because the spreading path of the part of the biological sample 1 spreading along the first guide sections 226 is longer than the spreading path of the middle part, the middle part of the biological sample 1 is able to spread ahead of the part that originally spreads faster along the first guide sections 226, so it is in the shape of a circular arc protrusion.

As shown in the schematic diagram (c) of FIG. 6 and in conjunction with FIG. 4 , when the biological sample 1 enters the middle section 222 of the chamber 22, the first concave arc 225 provides a smooth guide to prevent the biological sample 1 from turning and stopping due to the sharp corners. Therefore, the portion of the biological sample 1 that originally spreads faster along the inner side surfaces 227 is able to overtake the front middle portion, thus forming a circular arc concave shape.

As shown in the schematic diagram (d) of FIG6 and in conjunction with FIG4 , when the biological sample 1 enters the rear section 223 of the chamber 22, the second concave arcs 229 of the second guide surfaces 228 provide smooth guidance to prevent the biological sample 1 from turning and stopping due to the sharp corners, and to enable the portion of the biological sample 1 that originally spreads faster along the second guide surfaces 228 to continue to be ahead of the middle portion, and the advance becomes greater and greater, and the circular arc depression in the middle of the biological sample 1 becomes deeper and deeper (the more the biological sample 1 on the two side walls is ahead, the better, so that the subsequent enclosure and closing action can be performed).

As shown in the schematic diagram (e) of FIG. 6 and in conjunction with FIG. 4 , the second guide segments 220 are substantially orthogonal to the spreading direction X, so that the biological samples 1 flowing along the second guide segments 220 and the flow direction of the middle biological sample 1 are orthogonal to each other. In this way, the biological samples 1 on both sides can be effectively drawn to block in front of the middle biological sample 1 (enclosed and closed), so that the spreading speed of the biological samples 1 in the middle can be slowed down before the convergence, avoiding the risk of DNA fragmentation of the biological samples 1 due to collision, and also avoiding the middle biological sample 1 from spreading to the exhaust port 23 earlier than the biological samples 1 on both sides, causing vacuum bubbles on both sides of the chamber 22.

As shown in the schematic diagram (f) of FIG. 6 , the biological sample 1 flows to the exhaust port 23 .

Therefore, by utilizing the contour shape design of the chamber 22 of the present invention, it is possible to avoid the generation of bubbles during the process of the biological sample 1 being injected and spreading to the exhaust port 23 along the spreading direction X, and to achieve a better detection effect. In other words, by utilizing the design that each first guide surface 224 has the first concave arc 225 and the first guide section 226 extending from the first concave arc 225 toward the suction port 21, it is possible to provide smooth guidance and avoid the biological sample 1 turning and stopping due to the corner bend, so that the biological sample 1 can be quickly introduced into both sides of the chamber 22. Furthermore, by utilizing the cooperation of each second guide surface 228 having the second concave arc 229 and the second guide section 220 extending from the second concave arc 229 toward the exhaust port 23, the biological samples 1 on both sides are effectively drawn to gather in the middle in advance, so that the flow rate of the middle part of the biological sample 1 is slowed down, so that the biological samples 1 on both sides have a buffering effect when they converge relative to each other, avoiding the risk of the biological samples 1 on both sides colliding with the biological sample 1 in the middle and causing the DNA of the biological sample 1 to be broken, or the biological sample 1 in the middle flowing to the exhaust port 23 earlier than the biological samples 1 on both sides, causing vacuum bubbles to form on both sides of the chamber 22.

In summary, the biological sample 1 detection device of the present invention has a simple overall structure and can indeed solve the problem of vacuum bubbles generated when the biological sample 1 is injected, and can indeed achieve the purpose of the present invention.

However, the above is only an embodiment of the present invention and should not be used to limit the scope of implementation of the present invention. All simple equivalent changes and modifications made according to the scope of the patent application of the present invention and the content of the patent specification are still within the scope of the present patent.

1: Biological sample 10: Glass slide 11: Top surface 20: Interlayer 201: Recessed portion 202: Glue coating chamber 21: Inlet 211: Leading surface 212: First end surface 213: First inclined surface segment 214: First guide arc 22: Chamber 221: Front segment 222: Middle segment 223: Back segment 224: First guide surface 225: First concave arc 226: First guide segment 227: Inner surface 228: Second guide surface 229: Second concave arc 220: Second guide segment 23: Exhaust port 231: Back guide surface 232: End 233: Second inclined surface segment 234: Second guide arc 30: Cover glass X: Adsorption direction Y: Transverse axis R, R1, R2: Radius of curvature θ1: First angle θ2: Second angle 2: Slide 3: Interlayer 301: Rib 4: Cover glass 5: Counting chamber 501: Inlet 502: Chamber 503: Exhaust port 504: Front section 504’: First slope 505: Middle section 505’: Flat surface 506: Back section 506’: Second slope 6: Vacuum bubble

Other features and effects of the present invention will be clearly presented in the implementation method with reference to the drawings, in which: Figure 1 is a plan view schematic diagram of an existing biological sample detection device; Figure 2 is a three-dimensional assembly diagram of an embodiment of the biological sample detection device of the present invention; Figure 3 is a three-dimensional exploded diagram of the embodiment; Figure 4 is an incomplete plane enlarged schematic diagram of the embodiment; Figure 5 is a cross-sectional view along the line V-V in Figure 4; and Figure 6 is a schematic diagram of a biological sample adsorption state of the embodiment.

10: Glass slide

11: Top

20: Interlayer

201: Depression

21: Inlet

211: Leading surface

212: First end face

213: First inclined section

214: First guide arc

22: Chamber

221: First part

222: Middle section

223: The latter part

224: First guide surface

225: First concave arc

226: First introductory paragraph

227:Inner side

228: Second guide surface

229: Second concave arc

220: Second introductory paragraph

23: Exhaust port

231: Rear leading surface

232: The end

233: Second inclined section

234: Second guide arc

30: Cover slide

X: Adsorption direction

Y: horizontal axis

R, R1, R2: radius of curvature

θ1: first angle

θ2: Second angle

Claims (9)

A biological sample detection device, comprising: A glass slide, including a top surface; A partition layer, arranged on the top surface and including a recessed portion, the recessed portion having an inlet, a chamber connected to the inlet, and an exhaust port connected to the chamber, the exhaust port being arranged opposite to the inlet along a spreading direction, the chamber having a front section connected to the inlet and located on the inner side of the inlet along the spreading direction, a middle section connected to the front section and located on the inner side of the front section along the spreading direction, and a rear section between the middle section and the exhaust port, the front section being defined by a pair of first guide surfaces, the first guide surfaces extending along a transverse direction perpendicular to the spreading direction. The first guide surfaces are arranged opposite to each other and spaced apart, each of which has a first concave arc and a first guide section extending from the first concave arc toward the suction port, and a first angle is formed between the first guide sections. The rear section is defined by a pair of second guide surfaces, and the second guide surfaces are arranged opposite to each other and spaced apart along the transverse axis, each of which has a second concave arc and a second guide section extending from the second concave arc toward the exhaust port, and a second angle is formed between the second guide sections, and the second angle is greater than the first angle, and the second angle is between 140 degrees and 180 degrees; and a cover glass, which is spaced apart from the slide glass and is arranged on the spacer layer, and closes the chamber. A biological sample detection device as described in claim 1, wherein the second guide segments of the second guide surfaces of the spacer layer extend along the transverse axis, and the second angle is substantially 180 degrees, and the second guide segments are substantially orthogonal to the spreading direction. A biological sample detection device as described in claim 2, wherein the middle section of the spacer layer is defined by a pair of inner side surfaces, and the inner side surfaces are substantially parallel to the spreading direction. A biological sample detection device as described in claim 1, wherein the suction port of the spacer layer is defined by a pair of leading surfaces and a first end surface intersecting and connected between the leading surfaces, each leading surface has a first inclined surface segment, and a first guide arc connected between the first inclined surface segment and the corresponding first guide segment, and the first inclined surface segments gradually move away from each other when extending from the first end surface toward the corresponding first guide arc. A biological sample detection device as described in claim 4, wherein the first end surface of the suction port of the partition layer is arc-shaped. A biological sample detection device as described in claim 4, wherein the exhaust port of the spacer layer is defined by a pair of rear guide surfaces and an end, each rear guide surface has a second inclined surface segment, and a second guide arc connected between the second inclined surface segment and the corresponding second guide segment, and the second inclined surface segments gradually move away from each other when extending from the end toward the corresponding second guide arcs. A biological sample detection device as described in claim 1, wherein the first angle of the spacer layer is between 90 degrees and 110 degrees. A biological sample detection device as described in claim 1, wherein the curvature radius of the first concave arc of each first guide surface of the spacer layer is greater than the curvature radius of the second concave arc of each second guide surface. A biological sample detection device as described in claim 8, wherein the curvature radius of the first concave arc of each first guide surface of the spacer layer is 6 mm, and the curvature radius of the second concave arc of each second guide surface is 5 mm.

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