TWI859004B - Low-potassium medium and method for culturing cucumis melo l .var. reticulatus naud using the same - Google Patents

Abstract

A low-potassium medium is used for culturing a plant of Cucumis meloL .var. reticulatusNaud, reducing potassium ion level of the plant of Cucumis meloL .var. reticulatusNaud. Each liter of the potassium-free medium comprises: 77.9 mg of NO ₃-N, 0.0 mg of NH ₄-N, 23.8 mg of phosphorus (P), 124.2 mg of potassium (K), 73.4 mg of calcium (Ca), 41.7 mg of magnesium (Mg) and 71.4 mg of sulfur (S). A method for culturing the plant of Cucumis meloL .var. reticulatusNaud using the low-potassium medium is also disclosed.

Description

Low potassium culture solution and melon culture method using the same

The present invention relates to a low potassium culture solution, in particular to a low potassium culture solution for culturing melons. The present invention also relates to a melon culturing method using the low potassium culture solution.

Chronic kidney disease (CKD), also known as chronic kidney failure, is defined as kidney damage, i.e. glomerular filtration rate (GFR) less than 60 mL/min/1.73 ^m2 ; abnormalities found in blood tests, urine tests or imaging tests; and a history of more than three months. When the glomerular filtration rate drops below 15 mL/min/1.73 ^m2 , it means that the patient's kidneys cannot function normally and hemodialysis (commonly known as dialysis) is needed to replace the kidney's function.

Patients undergoing hemodialysis must strictly control their intake of potassium-containing foods, so the intake of fruits containing high amounts of potassium ions (e.g., melon, cantaloupe, pitaya, passion fruit, banana, kiwi, durian, papaya, lychee, etc.) will be strictly restricted. In view of this, a low-potassium melon culture method and a culture solution formula for use therewith should indeed be provided.

To solve the above problems, the main purpose of the present invention is to provide a low potassium culture solution for culturing low potassium melon.

Another object of the present invention is to provide a method for culturing low-potassium melon using the low-potassium culture solution as described above.

The low potassium culture solution for reducing the potassium ion content of melons of the present invention contains 77.9 mg of nitrate nitrogen, 0.0 mg of ammonium salt nitrogen, 23.8 mg of phosphorus, 124.2 mg of potassium, 73.4 mg of calcium, 41.7 mg of magnesium and 71.4 mg of sulfur per liter. In addition, the low potassium culture solution can also contain 2.6 mg of iron, 0.3 mg of manganese, 0.02 mg of copper, 0.05 mg of zinc, 0.004 mg of molybdenum, 0.001 mg of sodium and 0.54 mg of boron per liter.

Based on the same technical concept, the melon cultivation method of the present invention comprises: sowing a melon seed to obtain a melon plant; and culturing the melon plant with a potassium-containing culture solution for a growth period, followed by culturing the melon plant with a low-potassium culture solution for a treatment period. Preferably, the growth period is 46 days, and the treatment period is 39 days.

Based on this, by culturing the melon plant with the low potassium culture solution, the potassium ion concentration in the fruit of the melon plant can indeed be reduced, so that the fruit can be used as a source of fruit intake for hemodialysis patients, which is the effect of the present invention.

In order to make the above and other purposes, features and advantages of the present invention more clearly understood, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings as follows:

The "melon" referred to in the present invention is an annual herbaceous plant of the Cucurbitaceae family, an annual or biennial herbaceous plant of the genus Lactuca . The present invention uses the reticulated cantaloupe ( Cucumis melo L. var. reticulatus Naud.) with a reticulated surface, thick peel, and soft flesh for subsequent testing, but a person with ordinary knowledge in the technical field to which the present invention belongs should be able to select different varieties of melons for testing and adjustment according to needs, and there is no limitation here.

The melon cultivation method of the present invention is to sow a melon seed, and then obtain a melon plant after a seedling period, and then cultivate the melon plant with a potassium-containing culture solution during the growth period, and cultivate the melon plant with a low-potassium culture solution during the treatment period.

Specifically, the melon seeds were purchased from Ho Sheng Seedling Company and sown in a culture dish at 20°C for 24 hours of germination [days after sowing (DAS) = 0]. The germinated melon seeds were transferred to a sponge medium (soaked in water; DAS = 1) for cultivation. During the seedling period (DAS 1-12), LED tubes (light intensity of 160 μmol‧m ^-2 ‧s ^-1 ) were used for 24 hours of illumination, and the ambient temperature was kept constant at 26°C.

Then, on the 13th day after sowing (DAS=13), the growth phase began. The culture medium used was a potassium-containing culture medium. The growth phase lasted for 46 days. The culture conditions were different from those in the aforementioned seedling phase in that the light cycle was adjusted to 18 hours of light and 6 hours of dark, and the temperature during the light phase was maintained at 31°C, while the temperature during the dark phase was set at 25°C.

The drugs used in the potassium-containing culture medium are as follows: calcium nitrate (Ca(NO ₃ ) ₂ ‧4H ₂ O), potassium nitrate (KNO ₃ ), ammonium dihydrogen phosphate (NH ₄ H ₂ PO ₄ ), potassium dihydrogen phosphate (KH ₂ PO ₄ ), potassium sulfate (K ₂ SO ₄ ), magnesium sulfate (MgSO ₄ ‧7H ₂ O), sodium ferric ethylenediaminetetraacetate (C ₁₀ H ₁₂ N ₂ NaFeO ₈ ), boric acid (H ₃ BO ₃ ), manganese chloride (MnCl ₂ ‧4H ₂ O), zinc sulfate (ZnSO ₄ ‧7H ₂ O), copper sulfate (CuSO ₄ ‧5H ₂ O), and sodium molybdate (Na ₂ MoO ₄ ‧2H ₂ O), so that each liter of potassium-containing culture medium contains: 200.73 mg of nitrate nitrogen (NO ₃ －N), 17.36 mg of ammonium salt nitrogen (NH ₄ －N), 38.40 mg of phosphorus, 224.10 mg of potassium, 172.32 mg of calcium, 63.51 mg of magnesium and 83.79 mg of sulfur; in addition, each liter of low-potassium culture medium also contains 2.6 mg of iron, 0.3 mg of manganese, 0.02 mg of copper, 0.05 mg of zinc, 0.004 mg of molybdenum, 0.001 mg of sodium and 0.27 mg of boron and other trace elements. During the culture process, the pH of the potassium-containing culture medium was adjusted to 6.0 using 1 N sodium hydroxide (NaOH), and the conductivity of the potassium-containing culture medium was maintained at 1.2 mS‧cm ^-1 .

After the growth phase, the cells enter a treatment phase, in which the culture medium used is the low potassium culture medium of the present invention, and the treatment phase lasts for 39 days.

The drugs used in the low potassium culture solution are similar to those in the above-mentioned potassium-containing culture solution, except that ammonium nitrate (NH ₄ NO ₃ ) is used instead of potassium nitrate (KNO ₃ ), so that each liter of the low potassium culture solution contains: 77.9 mg of nitrogen nitrate (NO ₃ -N), 0.0 mg of ammonium salt nitrogen (NH ₄ -N), 23.8 mg of phosphorus, 156.0 mg of potassium, 73.4 mg of calcium, 41.7 mg of magnesium and 71.4 mg of sulfur; in addition, each liter of the low potassium culture solution also contains 2.6 mg of iron, 0.3 mg of manganese, 0.02 mg of copper, 0.05 mg of zinc, 0.004 mg of molybdenum, 0.001 During the culture process, 1 N sodium hydroxide (NaOH) was used to adjust the pH value of the low potassium culture solution to 6.0, and the conductivity of the low potassium culture solution was maintained at 1.2 mS‧cm ^-1 .

In order to verify that the potassium concentration of the melon plants can be effectively reduced by culturing the melon plants in the low potassium culture solution during the treatment period, the following test was conducted:

Please refer to Figure 1. After 12 days of seedling raising, the obtained melon plants were divided into CK group (control group, i.e., no treatment period), 0.20 K group, 0.15 K group, 0.10 K group and 0.05 K group (all experimental groups, and the content of each element is shown in Figure 1). The fruits of the melon plants were collected on the 97th day after sowing (DAS=97).

The types and contents of the collected fruits were then analyzed and recorded in Tables 1 to 3 below [data marked with different letters represent significant differences ( P < 0.05)].

Table 1 Group Fruit weight (g) Potassium ion concentration (mg/100 g fruit) (mg/fruit) CK 1110.4±121.4 ^ab 338.3±30.6 ^ab 3772.0±689.5 ^ab 0.20 K 610.2±44.9 ^d 368.3±23.6 ^a 2254.4±313.5 ^cd 0.15 K 570.5±157.7 ^d 343.3±43.7 ^ab 1915.9±283.7 ^d 0.10 K 696.5±215.7 ^cd 246.7±16.1 ^e 1695.1±440.8 ^d 0.05 K 950.3±210.2 ^bc 303.3±27.5 ^bcd 2911.8±855.9 ^bc

Table 2 Group Brix Titratable acid (%) Sugar-acid ratio Vitamin C content (mg/100 g fruit) CK 9.47±1.24 ^ab 0.066±0.018 ^abc 148.67±36.31 ^ab 0.63±0.11 ^ab 0.20 K 6.30±1.08 ^c 0.067±0.008 ^abc 93.17±5.35 ^b 0.56±0.13 ^abcd 0.15 K 6.80±1.49 ^bc 0.079±0.012 ^ab 86.99±20.26 ^b 0.62±0.10 ^ab 0.10 K 7.50±1.55 ^bc 0.058±0.014 ^ab 132.10±26.32 ^b 0.28±0.02 ^e 0.05 K 6.93±0.21 ^bc 0.039±0.023 ^d 261.32±220.02 ^a 0.38±0.08 ^cde

Table 3 Group Fruit length (mm) Fruit width (mm) Fruit length-to-width ratio Thickness of fruit (cm) CK 122.0±12.5 ^ab 118.5±3.0 ^ab 1.031±0.111 ^a 3.30±0.26 ^ab 0.20 K 104.3±4.9 ^c 106.5±2.8 ^bc 0.980±0.063 ^a 3.03±0.23 ^bc 0.15 K 104.8±5.3 ^c 101.6±11.5 ^c 1.036± ^0.065a 2.73±0.21 ^bc 0.10 K 107.0±14.3 ^bc 107.3±14.3 ^bc 0.994±0.058 ^a 2.57±0.15 ^c 0.05 K 117.1±7.0 ^abc 115.0±6.8 ^ab 1.019±0.024 ^a 2.93±0.15 ^bc

As shown in Table 1, the fruit weight and potassium ion concentration (mg/100 g fruit) decreased as the potassium content of the low potassium culture solution used decreased. Compared with the CK group, the potassium ion concentration per fruit in the 0.20 K group using the low potassium culture solution of the present invention decreased significantly.

As shown in Table 2, compared with the CK group, the sugar-acid ratio and vitamin C content of the fruit in the 0.20 K group using the low potassium culture solution of the present invention were not significantly different.

As shown in Table 3, there were no significant differences in the fruit length-to-width ratio and fruit thickness of the 0.20 K group using the low potassium culture solution of the present invention compared to the CK group.

In summary, by culturing the melon plant in the low-potassium culture solution, the potassium ion concentration in the fruit of the melon plant can indeed be reduced, so that the fruit can be used as a fruit intake source for hemodialysis patients, which is the effect of the present invention.

Although the present invention has been disclosed using the above preferred embodiments, they are not intended to limit the present invention. Any person skilled in the art may make various changes and modifications to the above embodiments without departing from the spirit and scope of the present invention, and the scope of protection of the present invention shall be subject to the scope of the attached patent application.

without

[Figure 1]      Element content distribution diagram of each experimental group of the present invention.

Claims (4)

A low potassium culture solution for reducing the potassium ion content of melon, wherein each liter of the low potassium culture solution contains: 77.9 mg of nitrate nitrogen, 0.0 mg of ammonium salt nitrogen, 23.8 mg of phosphorus, 124.2 mg of potassium, 73.4 mg of calcium, 41.7 mg of magnesium and 71.4 mg of sulfur. For example, the low potassium culture solution for reducing the potassium ion content of melon in claim 1, each liter of the low potassium culture solution further contains: 2.6 mg of iron, 0.3 mg of manganese, 0.02 mg of copper, 0.05 mg of zinc, 0.004 mg of molybdenum, 0.001 mg of sodium and 0.54 mg of boron. A method for cultivating melons comprises: sowing a melon seed to obtain a melon plant; and cultivating the melon plant with a potassium-containing culture solution for a growth period, and then cultivating the melon plant with a low-potassium culture solution for a treatment period; wherein each liter of the potassium-containing culture solution contains 200.73 mg of nitrate nitrogen, 17.36 mg of ammonium salt nitrogen, 38.40 mg of phosphorus, 224.10 mg of Potassium, 172.32mg calcium, 63.51mg magnesium and 83.79mg sulfur. Each liter of the low potassium culture solution contains 77.9mg nitrate nitrogen, 0.0mg ammonium nitrogen, 23.8mg phosphorus, 124.2mg potassium, 73.4mg calcium, 41.7mg magnesium and 71.4mg sulfur. The melon cultivation method of claim 3, wherein the number of days in the growth period is 46 days and the number of days in the treatment period is 39 days.