TWI849643B - Improved structure of quantitative molecular detection chip - Google Patents

Abstract

The present invention discloses an improved structure of a quantitative molecular detection chip, which mainly includes a base, a reaction tank and a light-transmitting portion, wherein the reaction tank is arranged on the base to accommodate a test object; the light-transmitting portion has a curved surface separated from the reaction tank, and the optical focus point of the curved surface is located within the tank space range of the reaction tank, so that a light ray can be focused in the reaction tank after passing through the curved surface.

Description

Improved structure of quantitative molecular detection chip

The present invention is related to biochip technology, in particular to a quantitative molecular detection system, an improved structure of a quantitative molecular detection chip and a method for manufacturing the chip.

According to the microelectromechanical technology, biochips are used to perform biological reactions or analyses, such as gene expression analysis, disease diagnosis, drug screening, gene sequencing, protein analysis, etc. There are many types of biochip detection methods, such as optical spectroscopy, electrochemical detection, mass spectrometry, etc. Among them, optical spectroscopy mainly uses light of a specific wavelength to illuminate the sample to be tested, so as to receive the excited scattered light or reflected light and perform analysis.

However, as mentioned above, although the existing technology uses optical detection for detection, its detection effect is not good. Most of the reasons are that optical detection is more sensitive to the intensity of the light wave signal of scattered or reflected light. If the intensity is slightly insufficient, it may affect the accuracy of the detection result.

Based on this, how to provide a detection method that can ensure the intensity of light wave signals without increasing the cost of equipment, that is, without increasing the intensity of the existing light source, will require further improvement and research and development.

Therefore, the main purpose of the present invention is to provide an improved structure of a quantitative molecular detection chip, which can focus the light emitted by an external light source into a test area on the detection chip to increase the light wave signal intensity of the stimulated reflected light and improve the problem of poor detection effect of the known technology.

Therefore, in order to achieve the above-mentioned purpose, the improved structure of the quantitative molecular detection chip provided by the present invention mainly includes a base, a reaction tank and a light-transmitting portion, wherein the reaction tank is arranged on the base to accommodate a test object; the light-transmitting portion has a curved surface separated from the reaction tank, and the optical focusing point of the curved surface is located within the tank space range of the reaction tank, so that a light ray can be focused in the reaction tank after passing through the curved surface.

In one embodiment, the reaction tank wall and the bottom are formed with a first hydrophilic surface to prevent the generation of bubbles when the test object is placed in the reaction tank, thereby affecting the uniformity of the reaction temperature, the accuracy of optical detection, or causing leakage.

In one embodiment, the first hydrophilic surface is formed by plasma treatment.

In one embodiment, the curved surface of the light-transmitting portion is formed on the outer peripheral contour surface of the base.

In one embodiment, the light-transmitting portion and the base are integrally formed.

In one embodiment, the curved surface uses the center of the reaction tank as the optical focal point.

In one embodiment, the reaction tank is in the shape of a circular tube, and the center of curvature of the circular tube is used as the optical focusing point of the curved surface.

In one embodiment, the base has a first side, and the notch of the reaction tank is located on the first side, and the present invention further includes a packaging part, which is arranged on the first side of the base to close the notch of the reaction tank.

In one embodiment, the packaging portion includes a film layer and a first adhesive layer, wherein the film layer covers the first side of the base; the first adhesive layer is between the film layer and the base to bond the film layer to the base.

In one embodiment, the base has a plate body, and the plate body has a plate body, a first side, a second side, an injection concave hole, an injection flow channel, an outflow concave hole and an outflow flow channel, and the first side and the second side are respectively located on two opposite end surfaces of the plate body, and the reaction tank is recessed on the first side of the plate body, and the injection concave hole is recessed on the plate body to be separated from the reaction tank. The injection concave hole is disposed on the first side of the plate body, and the injection channel is disposed between the injection concave hole and the reaction tank to connect the injection concave hole and the reaction tank, and the outflow concave hole is disposed on the first side of the plate body away from the injection concave hole and separated from the reaction tank, and the outflow channel is disposed between the outflow concave hole and the reaction tank to connect the outflow concave hole and the reaction tank.

In one embodiment, the packaging part includes a base, a first flow channel and a second flow channel, wherein the first flow channel and the second flow channel are respectively arranged on the base corresponding to the positions of the injection concave hole and the outflow concave hole, and the inner wall surface of the first flow channel and the inner wall surface of the second flow channel are respectively formed with a second hydrophilic surface; accordingly, when the base is arranged on the first side of the base, the first flow channel and the second flow channel are respectively connected to and communicated with the injection concave hole and the outflow concave hole.

In one embodiment, the second hydrophilic surface is formed by plasma treatment.

In one embodiment, the packaging portion further includes a second adhesive layer between the seat and the base to bond the seat to the base.

In one embodiment, the packaging part further includes a plug body, which is detachably embedded in an opening opened at one end of the base body away from the flow channel to seal the connection between the flow channel and the outside.

In one embodiment, the base has a second side opposite to the first side; and the present invention further includes a temperature adjustment portion and a sensing portion, wherein the temperature adjustment portion is disposed on the second side of the base corresponding to the position of the reaction tank to adjust the temperature of the object to be tested contained in the reaction tank; the sensing portion is electrically connected to the temperature adjustment portion and is located adjacent to the temperature adjustment portion.

In one embodiment, the temperature adjustment part and the sensing part are arranged on a body and are electrically connected to a processing part via a feedback circuit respectively, and the processing part receives the signal detected by the sensing part to control the operation of the temperature adjustment part.

In one embodiment, the temperature regulating part has a planar heating and cooling loop, which is arranged in the area overlapping the reaction tank on the body in accordance with the shape of the reaction tank.

In one embodiment, the sensing portion has a temperature sensing point, which is arranged on the main body corresponding to the location of the reaction tank, and the heating coil surrounds the temperature sensing point.

Furthermore, the present invention also provides a quantitative molecular detection system, which includes a machine, a chip as described above, a light source, an image capture unit and a heat sink, wherein the chip is disposed on the machine; the light source is disposed on the machine, and an incident light generated by the light source is irradiated on the reaction tank on the chip; the image capture unit is disposed on the machine and receives a fluorescent light excited by the incident light; the heat sink is disposed at a position of the machine adjacent to the chip to cool the chip.

In addition, the present invention further provides a method for manufacturing a quantitative molecular detection chip, which includes the following steps: Step A: Take the base as described above; Step B: Perform plasma treatment on the wall of the reaction tank provided on the base to form a first hydrophilic surface; Step C: Take a patch and cover it on the base, and the patch carries a temperature regulating part to heat and cool the test object contained in the reaction tank; Step D: Use a packaging technology to seal the slot of the reaction tank.

In one embodiment, the packaging technology is to form a first adhesive layer on a film layer and affix one side of the first adhesive layer to the base to bond the film layer to the base and thereby seal the reaction tank.

In one embodiment, the packaging technology is to set a liquid injection seat on the base, and the liquid injection seat has a seat body and a flow channel penetrating the seat body. When the seat body is set on the base, the flow channel is connected to the reaction tank; a plug body is embedded in the flow channel to close the connection between the flow channel and the outside.

In one embodiment, the inner wall surface of the flow channel is further subjected to plasma treatment to form a second hydrophilic surface.

In one embodiment, when the base is disposed on the base, a second adhesive layer is interposed between the base and the base to bond the base to the base.

10: Quantitative molecular detection chip

10A, 10B: Chip

11: Base

111, 111A: Plate

1111: Board body

1112, 1112A: First side

1113: Second side

1115, 1115A: injection concave hole

1116, 1116A: Injection channel

1117, 1117A: outflow concave hole

1118, 1118A: Outflow channel

12: Induction heating and cooling unit

121:Entity

122: Temperature control unit

123:Sensor unit

124: Feedback circuit

13, 13A: Packaging Department

131: Membrane layer

132: First adhesive layer

133: Seat

1331: Substrate

1332: First pipe fitting

1333: Second pipe fitting

1334: First through hole

1335: Second through hole

134: First flow channel

135: Second adhesive layer

136: First plug

137: Second flow channel

138: Second plug

14, 14A: Reactor

15: Light-transmitting part

20, 20B: Processing Department

30: Machine

40: Light source

50: Image capture unit

60: Heat dissipation part

Figure 1 is a three-dimensional assembly diagram of the quantitative molecular detection chip provided by the first embodiment of the present invention.

Figure 2 is a three-dimensional exploded view of the quantitative molecular detection chip of the first embodiment of the present invention.

Figure 3 is a cross-sectional view taken along section line 3-3 of Figure 1.

Figure 4 is a system block diagram of a specific usage pattern of the first embodiment of the present invention.

Figure 5 is a three-dimensional assembly diagram of the quantitative molecular detection chip provided by the second embodiment of the present invention.

Figure 6 is a three-dimensional exploded view of the quantitative molecular detection chip provided by the second embodiment of the present invention.

Figure 7 is a cross-sectional view taken along section line 7-7 of Figure 5.

Figure 8 is a schematic diagram of the quantitative molecular detection system provided by the third embodiment of the present invention.

FIG9 is a system block diagram of the quantitative molecular detection system provided by the third embodiment of the present invention.

First, please refer to Figures 1 to 3, which are the quantitative molecular detection chip 10 provided in the first embodiment of the present invention, which mainly includes a base 11, an inductive heating and cooling unit 12, a packaging part 13, a reaction tank 14 and a light-transmitting part 15.

The base 11 has a plate 111, and the plate 111 has a plate body 1111, a first side 1112, a second side 1113, an injection concave hole 1115, an injection channel 1116, an outflow concave hole 1117 and an outflow channel 1118, wherein the plate body 1111 is a hard plastic material, such as polycarbonate (PC), and is transparent to allow visible light to pass through, and the first side 1112 and the second side 1113 are respectively located on two opposite end surfaces of the plate body 1111.

Furthermore, the reaction tank 14 is recessed on the first side 1112 of the plate body 1111 to accommodate an object to be tested. In other words, the reaction tank 14 in this example is a circular tube structure, and in other possible implementations, the shape surrounded by the tank wall of the reaction tank 14 can be any other shape, such as a rectangle, a rhombus, a polygon, etc.

The injection concave hole 1115 is recessed on the first side 1112 of the plate body 1111 to be separated from the reaction tank 14, and the injection channel 1116 is provided between the injection concave hole 1115 and the reaction tank 14 to connect the injection concave hole 1115 and the reaction tank 14, and the outflow concave hole 1117 is recessed on the first side 1112 of the plate body 111 away from the injection concave hole 1115 and separated from the reaction tank 14, and the reaction tank 14 is interposed between the injection concave hole 1115 and the outflow concave hole 1117, and the outflow channel 1118 is provided between the outflow concave hole 1117 and the reaction tank 14 to connect the outflow concave hole 1117 and the reaction tank 14.

The inductive heating and cooling unit 12 has a body 121, a temperature adjustment part 122, a sensing part 123 and a feedback circuit 124, wherein the body 121 is a flexible polyimide film (PI) and is designed to match the outer contour shape of the plate 111, and its thickness is 0.025mm, and the body 121 is attached to the second side 1113 of the plate 111. The sensing part 123 has a temperature sensing point, which is arranged on the side of the body 121 facing the plate 111 corresponding to the position of the reaction tank 14. The temperature regulating portion 122 has a planar heating and cooling loop, which is arranged on one side of the main body 121 facing the plate 111 and in an area overlapping the reaction tank 14 in accordance with the shape of the reaction tank 14, and the heating coil surrounds the temperature sensing point. In other words, the planar heating and cooling loop is centered on the temperature sensing point and is divided into a plurality of concentric circles with a diameter between 8 mm and 10 mm. In addition, the planar heating loop simultaneously detects the temperature of the object to be tested carried in the reaction tank 14 using the T-type thermocouple principle. The feedback circuit 124 is disposed on the main body 121 using the existing flexible circuit process, and a plurality of pins are formed on one side of the main body 121 to electrically connect the temperature adjustment part 122 and the sensing part 123 to an external processing part 20, as shown in FIG. 4 . The processing part 20 receives the temperature signal detected by the sensing part 123 to control the operation of the temperature adjustment part 122 . Furthermore, the material of the feedback circuit 124 can be copper, constantan, indium tin oxide, metal, conductive carbon material or a combination thereof. In this example, copper and constantan are selected, and the temperature adjustment part 122 and the sensing part 123 are integrated by using eutectic bonding technology. The eutectic bonding technology is a known technology, so it is not repeated here. In addition, one side of the body 121 having the feedback circuit 124, the temperature adjustment part 122 and the sensing part 123 can be further coated with a layer of polyimide film.

According to the above description, the inductive heating and cooling unit 12 is used as a temperature sensing and temperature control patch and is attached to the base 11.

The packaging part 13 is transparent, allows visible light to pass through, and includes a film layer 131 and a first adhesive layer 132, wherein the film layer 131 is a polymer film with a thickness of 0.05 mm, fixed and covered on the first side 1112 of the plate 111, so as to simultaneously seal the reaction tank 14, the injection concave hole 1115, the injection channel 1116, the outflow concave hole 1117 and the outflow channel 1118. And allow the test object and the reaction reagent to penetrate and enter the reaction tank 14. Furthermore, the first adhesive layer 132 is a polymer sealing film with a thickness of 0.125 mm, and covers the film layer 131 to seal the injection hole of the film layer and provide optical transparency. In addition, the fixing technology between the film layer 131 and the plate body 111 is not limited to the aforementioned bonding method, and other fixing methods such as ultrasonic sealing, glue sealing, etc. can be used to achieve the same effect.

Furthermore, the wall and bottom of the reaction tank 14, the wall of the injection recess 1115, the wall of the injection channel 1116, the wall of the outflow recess 1117 and the wall of the outflow channel 1118 respectively form a first hydrophilic surface. In other words, the first hydrophilic surface can be provided on the surface of the chip for the sample to flow through or store. The first hydrophilic surface is formed by plasma treatment, and the best embodiment of the plasma is oxygen plasma, and the second best is argon plasma. Under the action of light and ions in the plasma, the surface of the base 11 generates free radicals, and the free radicals are very active and can easily react chemically with substances, so they can be used as a medium to bond other substances. Specifically, the water contact angle test results of the surface without plasma modification and the first hydrophilic surface are shown in the following table, wherein the first hydrophilic surface is modified under the first condition and the second condition respectively. The first condition refers to the selected voltage of 100W, the fixed oxygen flow rate of 50sscm, and the test time of 5 minutes, while the second condition refers to the selected voltage of 200W, the fixed oxygen flow rate of 50sscm, and the test time of 15 minutes. From the data in the table below, it can be seen that the hydrophilicity of the first hydrophilic surface under different conditions is significantly better than that of other surfaces without plasma modification. Accordingly, when the sample flows through the first hydrophilic surface, the generation of bubbles can be avoided to maintain the uniformity of the reaction temperature, the accuracy of optical detection, or to avoid leakage.

The light-transmitting portion 15 has a curved surface separated from the reaction tank 14, and the curved surface is integrally formed on the outer peripheral contour surface of the plate body 111, and the optical focal point of the curved surface is located within the tank space range of the reaction tank 14. When a light passes through the curved surface, the light is focused in the reaction tank 14. In other words, the curved surface uses the center of the reaction tank 14 as the optical focal point, so that the light is converged on the reaction tank 14. In addition, the spot range of the light can cover an area of at least 5 mm around the periphery of the reaction tank 14.

Accordingly, through the above structural description, the specific manufacturing steps of the quantitative molecular detection chip 10 of the present invention are as follows: Step A: Take the base 11 as mentioned above; Step B: Perform plasma treatment on the groove wall of the reaction groove 14 set on the base 11 to form a first hydrophilic surface; Step C: Take the temperature sensing heating and cooling patch and cover it on the base 11, and the temperature sensing heating and cooling patch is equipped with the temperature adjustment part 122, which is used to heat the test object contained in the reaction groove 14 and cool it down by air cooling with the high thermal conductivity of the metal loop; Step D: Use packaging technology to seal the groove of the reaction groove 14. The packaging technology is to form a first adhesive layer 132 on one side of the film layer 131 and adhere it to the base 11 to bond the film layer 131 to the base 11 and seal the reaction tank 14.

As shown in FIG. 5 and FIG. 7, the second embodiment of the present invention is disclosed. The main difference between the chip 10A disclosed therein and the first embodiment is that the package portion 13A is used as a liquid injection seat. Specifically, the package portion 13A includes a seat body 133, a first flow channel 134, a second adhesive layer 135, a first plug body 136, a second flow channel 137 and a second plug body 138. The seat body 133 has a substrate 1331, a first tube 1332 and a second tube 1333. The first tube 1332 is a first tube 1333. 332 is corresponding to the position of the injection recessed hole 1115A, and is penetrated on one side of the substrate 1331 according to its tube axis perpendicular to the plate surface of the substrate 1331, and a first through hole 1334 connected to the internal space of the first tube 1332 is opened on the other side of the substrate 1331, so that the internal space of the first tube 1332 and the first through hole 1334 jointly define the first flow channel 134, and the aperture size of the first through hole 1334 is similar to the aperture size of the injection recessed hole 1115A. Furthermore, the second pipe 1333 is arranged on one side of the substrate 1331 with its pipe axis perpendicular to the surface of the substrate 1331, corresponding to the location of the outflow concave hole 1117A, and a second through hole 1335 connected to the inner space of the second pipe 1333 is opened on the other side of the substrate 1331, so that the inner space of the second pipe 1333 and the second through hole 1335 jointly define the second flow channel 137, and the aperture size of the second through hole 1335 is similar to the aperture size of the outflow concave hole 1117A.

Furthermore, a second hydrophilic surface is formed on the inner wall surface of the first tube 1332 and the inner wall surface of the second tube 1333, respectively, and the second hydrophilic surface is also formed by the same plasma treatment technology as mentioned above.

Furthermore, the second adhesive layer 135 is disposed on the substrate 1331 using screen printing technology and is located on the same side as the first through hole 1334 and the second through hole 1335. The screen printing process refers to a printing technology using a screen mask to perform screen printing for precise pattern coating. For example, the second adhesive layer 135 is used in an appropriate amount in accordance with the contour of the reaction tank 14A, and the second adhesive layer 135 is intermittently disposed on the substrate 1331. In other possible implementations, the second adhesive layer 135 can also be disposed on the first side 1112A of the plate body 111A and around the reaction tank 14A, which can also achieve the function of bonding the base body 133 and the plate body 111A. In addition, the fixing technology between the base 133 and the plate 111A is not limited to the aforementioned bonding method, and other fixing methods can be used to achieve the same effect.

Next, when the seat body 133 is disposed on the first side 1112A of the plate body 111A, the substrate 1331 is covered on the first side 1112A of the plate body 111A to simultaneously close the reaction tank 14A, the injection channel 1116A, and the outflow channel 1118A, and the first through hole 1334 and the second through hole 1335 are respectively connected to the orifice of the injection recess 1115A and the orifice of the outflow recess 1117A, so that the first channel 134 is connected to the injection recess 1115A, and the second channel 137 is connected to the outflow recess 1117A. In addition, the second adhesive layer 135 is located between the base 133 and the plate 111A to bond the base 133 to the plate 111A.

Based on this, taking the implementation of the injection of the test object as an example, the nozzle of the syringe used for the injection of the test object is inserted into the first tube 1332 of the seat 133 for insertion, and the nozzle and the first tube 1332 have a suitable degree of close contact to ensure that the injected test object does not produce improper infiltration, and the test object enters the reaction tank 14A in sequence through the first flow channel 134, the injection concave hole 1115A and the injection flow channel 1116A.

The first plug 136 is detachably disposed in an opening at one end of the first pipe 1332 away from the plate 111A to seal the connection between the first flow channel 134 and the outside.

The second plug 138 is detachably embedded in an opening of the second pipe 1333 away from the plate 111A to seal the connection between the second flow channel 137 and the outside.

Furthermore, the first pipe member 1332 and the second pipe member 1333 are provided with a non-return structure on the inner wall respectively, so as to be embedded with the first plug body 136 and the second plug body 138 to increase the sealing property of the injection recessed hole 1115A and the outflow recessed hole 1117A.

In addition, the packaging part 13A defined in the second embodiment can be applied to the packaging technology used in manufacturing the quantitative molecule detection chip described in the first embodiment to perform another type of packaging operation.

As shown in FIG8 and FIG9, the quantitative molecular detection system provided in the third embodiment of the present invention includes the chip 10B, the processing unit 20B, a machine 30, a light source 40, an image capture unit 50, and a heat sink 60, wherein the light source 40, the image capture unit 50, the heat sink 60 and the processing unit 20B are directly or indirectly transmitted with signals by any wired or wireless media (e.g. 4G, 5G, WIFI, Bluetooth, NFC, RFID, etc.).

The machine 30 is a basic structure for carrying other components. In short, the machine 30 in this example is mainly composed of four plates assembled together to set the chip 10B, the light source 40, the image capture unit 50, the heat dissipation unit 60 and the processing unit 20B.

The chip 10B is taken from the quantitative molecular detection chip described in the first embodiment or the second embodiment, and is used to carry the test object. The test object in this example includes biological samples and PCR reagents, wherein the biological sample refers to a sample of an organism (Organism) derived from humans or other animals, or a sample of components (Components) of its cells or tissues, such as blood (Blood), plasma (Plasma), serum (Serum), urine (Urine), saliva (Saliva) or feces (Feces), etc.

Furthermore, the biological sample is fluorescently labeled with the PCR reagent, and commonly used fluorescent labels may be but are not limited to FAM, HEX, TET, TAMRA, Cy3, Cy5, Cy5.5, Texas Red, VIC, Yakima Yellow, BHQ-1, BHQ-2 or BHQ-3, where FAM is green light, HEX is yellow light, and TAMRA is red light. In this example, FAM is used for fluorescent labeling, and the fluorescence generated by its excitation is the green light band of the excitation light with a peak at 520nm.

The light source 40 can be, but is not limited to, any known light-emitting element of red, green, blue or any other color. In this example, the light source 40 uses a light-emitting diode (LED) of model VISHAY 78-VLDB1232G-08, and the light it emits is blue light with a wavelength between 458nm and 472nm, preferably with a peak of 465nm. Furthermore, when the blue light is irradiated on the chip 10B, its incident angle can be any angle, preferably 45°. Then, the fluorescence generated by the excitation of the object to be tested is a green light band of the excitation light with a peak at 520nm, and is captured by the image capture unit 50. In particular, the blue light can be focused into the reaction tank through the curved surface to increase the intensity of the light wave signal of the stimulated fluorescence, thereby improving the problem of poor detection effect of the known technology. In addition, the blue light can penetrate the packaging part 13 and enter the reaction tank 14 from the first side 1112 of the plate body 111.

The image capture unit 50 may be, but is not limited to, a charge coupled device (CCD), a complementary metal-oxide semiconductor (CMOS) element or other types of photosensitive elements, which are used to receive the fluorescence to generate a light wave signal and transmit it to the processing unit 20B. In addition, a filter is provided between the image capture unit 50 and the chip 10B to pass light of a specific wavelength to exclude other external interference signals.

The processing unit 20B has one or more microprocessors, central processing units (CPUs), computing devices, microcontrollers, digital signal processors, graphics processing units (GPUs), other similar devices with computing functions or any combination of their groups, and receives the light wave signal to perform detection and analysis, and the detection and analysis is performed by fluorescence detection, and in other possible implementations, it can also be a reflectivity measurement method or an absorptivity detection method, etc.

The heat dissipation unit 60 is a blowing device, which is fixedly arranged at a position adjacent to the chip 10B, and the blowing device includes a motor and a fan driven by the motor, and the air outlet direction of the fan is toward the chip 10B, and is controlled by the processing unit 20B to perform air cooling on the chip 10B. For example, the fan is started during the process of controlling the thermal cycle from the high temperature denaturation temperature to the low temperature bonding temperature, and the fan is stopped when the temperature of the chip 10B drops to the set bonding temperature.

Accordingly, the quantitative molecular detection system of the present invention can be used for qPCR (Real-time polymerase chain reaction) detection in practical applications. Although the above description refers to illustrative embodiments for specific applications, it should be understood that the claimed invention is not limited to such embodiments. Those skilled in the art will recognize additional modifications, applications, and embodiments within the scope of the attached patent application by reading the teachings provided herein.

10: Quantitative molecular detection chip

111: Board

121:Entity

131: Membrane layer

14: Reactor

15: Light-transmitting part

Claims (15)

An improved structure of a quantitative molecular detection chip includes: a base having a first side; a reaction tank disposed on the base, and the notch of the reaction tank is located on the first side for accommodating a test object; a light-transmitting portion having a curved surface separated from the reaction tank, and the optical focus point of the curved surface is located within the tank space of the reaction tank, so that a light ray can be focused in the reaction tank after passing through the curved surface; a packaging portion is disposed on the first side of the base to seal the notch of the reaction tank; wherein the packaging portion includes: a film layer covering the first side of the base; a first adhesive layer between the film layer and the base to bond the film layer to the base. The improved structure of the quantitative molecular detection chip as described in claim 1, wherein the reaction tank wall and the tank bottom form a first hydrophilic surface. The improved structure of the quantitative molecular detection chip as described in claim 2, wherein the first hydrophilic surface is formed by plasma treatment. The improved structure of the quantitative molecular detection chip as described in claim 1, wherein the curved surface of the light-transmitting portion is integrally formed on the outer peripheral contour surface of the base. The improved structure of the quantitative molecular detection chip as described in claim 1, wherein the optical focal point of the curved surface is located at the center of the reaction tank. The improved structure of the quantitative molecular detection chip as described in claim 1, wherein the reaction tank is in the shape of a circular tube, and the center point of the curvature of the circular tube shape and the optical focal point of the curved surface are at the same position. The improved structure of the quantitative molecular detection chip as described in claim 1, wherein the base has a plate body, and the plate body has a plate body, a first side, a second side, an injection concave hole, an injection flow channel, an outflow concave hole and an outflow flow channel, and the first side and the second side are respectively located on two opposite end surfaces of the plate body, and the reaction groove is recessed on the first side of the plate body, and the injection concave hole is opposite to the reaction groove. The injection concave hole is separated from the reaction tank and is recessed on the first side of the plate body, and the injection channel is provided between the injection concave hole and the reaction tank to connect the injection concave hole and the reaction tank, and the outflow concave hole is far away from the injection concave hole and is separated from the reaction tank and is recessed on the first side of the plate body, and the outflow channel is provided between the outflow concave hole and the reaction tank to connect the outflow concave hole and the reaction tank. The improved structure of the quantitative molecular detection chip as described in claim 7, wherein the packaging part includes: a base; a first flow channel and a second flow channel, which are respectively arranged on the base corresponding to the positions of the injection concave hole and the outflow concave hole, and the inner wall surface of the first flow channel and the inner wall surface of the second flow channel are respectively formed with a second hydrophilic surface; wherein, when the base is arranged on the first side of the base, the first flow channel and the second flow channel are respectively connected to and communicated with the injection concave hole and the outflow concave hole. The improved structure of the quantitative molecular detection chip as described in claim 8, wherein the second hydrophilic surface is formed by plasma treatment. The improved structure of the quantitative molecular detection chip as described in claim 8, wherein the packaging part further includes a second adhesive layer between the seat and the base to bond the seat to the base. The improved structure of the quantitative molecular detection chip as described in claim 10, wherein the packaging part further includes a plug body, which is detachably disposed in an opening opened at one end of the base body away from the flow channel to seal the connection between the flow channel and the outside and prevent the liquid in the flow channel from flowing back. An improved structure of a quantitative molecular detection chip includes: a base having a first side and a second side opposite to each other; a reaction tank disposed on the base, and the notch of the reaction tank is located on the first side, for accommodating a test object; a light-transmitting portion having a curved surface separated from the reaction tank, and the optical focus of the curved surface is located within the tank space of the reaction tank, so that a light ray passing through the curved surface can be focused on the reaction object. After the surface is formed, the light can be focused into the reaction tank; a packaging part is arranged on the first side of the base to seal the notch of the reaction tank; and a temperature adjustment part is arranged on the second side of the base corresponding to the position of the reaction tank to adjust the temperature of the object to be tested contained in the reaction tank; a sensing part is electrically connected to the temperature adjustment part and is located adjacent to the temperature adjustment part. The improved structure of the quantitative molecular detection chip as described in claim 12, wherein the temperature adjustment part and the sensing part are arranged on a body and are electrically connected to a processing part by a feedback circuit respectively, and the processing part receives the signal detected by the sensing part to control the operation of the temperature adjustment part. The improved structure of the quantitative molecular detection chip as described in claim 13, wherein the temperature adjustment portion has a planar heating and cooling loop, which is arranged in the area on the body overlapping with the bottom of the reaction tank in accordance with the shape of the reaction tank. The improved structure of the quantitative molecular detection chip as described in claim 14, wherein the sensing portion has a temperature sensing point, which is arranged on the body corresponding to the location of the reaction tank, and the heating coil surrounds the temperature sensing point.

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