TWI788850B - Biomechanical testing system and its reactor module - Google Patents

Abstract

A biomechanical testing system and its reactor module. The biomechanical testing system includes a reactor module, a storage barrel containing liquid and connected to the reactor module, and an air pressure source connected to the storage barrel. The reactor module includes an upper template, a lower template, a positioning plate arranged between the upper template and the lower template, a spacer, and at least one biological cultivation material. The upper template, the lower template, and the positioning plate cooperate to define a closed space for accommodating the limiting part and the at least one biological cultivation material. The air pressure source can be controlled to provide gas to the storage tank, so that the liquid in the storage tank enters the closed space through air pressure and flows through the at least one biological culture material to simulate fluid pressure and fluid flow in organisms And the situation of pulse.

Description

Biomechanical testing system and its reactor module

The present invention relates to a biomechanical test system, in particular to a biomechanical test system for simulating a fluid-related environment in physiology for biomechanical tests (such as blood circulation system, renal excretion system).

The blood vessels in the human or animal body are intricate, and they have different diameters and different angles of bifurcations depending on the location and connection conditions. In the journal "Neurosurgery" in August 2018, scholar Tetsuo Sasaki used computational fluid dynamics ( Computational Fluid Dynamics (CFD for short) to simulate the vascular bifurcation model, and clarified how the geometry of the bifurcation site affects the formation of aneurysms. However, purely theoretical calculation methods are still insufficient in accuracy, and it is difficult to quickly simulate different biological environments. In addition to blood vessels, other types of biomechanical detection also have this problem, so there is still room for improvement.

Therefore, the object of the present invention is to provide a biomechanical test system that can actually simulate the fluid-related environment in physiology.

Therefore, the biomechanical testing system of the present invention includes a reactor module, a storage tank containing liquid and connected to the reactor module, and an air pressure source connected to the storage tank. The reactor module includes an upper template, a lower template arranged below the upper template, a positioning plate arranged between the upper template and the lower template, a positioning plate surrounded by the positioning plate and located on the upper template and the A limiting piece between the lower templates, and at least one pair of biological culture materials set by the limiting piece. The upper template, the lower template, and the positioning plate cooperate to define a closed space for accommodating the limiting part and the at least one biological cultivation material. The storage tank can be controlled to supply liquid to the enclosed space. The air pressure source can be controlled to provide air to the storage tank, so that the liquid in the storage tank enters the closed space through the air pressure and flows through the at least one biological culture material.

Another object of the present invention is to provide a reactor module in the biomechanical test system.

Therefore, the reactor module of the present invention includes an upper template, a lower template arranged below the upper template, a positioning plate arranged between the upper template and the lower template, a limiting member, and at least a pair of The biological culture material that should be set by the limit piece. The positioning plate, the upper template, and the lower template cooperate to define a closed space. The limiting part and the at least one biological culture material are arranged in the closed space, surrounded by the positioning plate and located between the upper template and the lower template.

The effect of the present invention is that: the liquid contained in the storage barrel is usually a culture solution, which will enter the closed space after being pressurized by the air pressure source, and flow through the at least one biological culture material, so that the fluid can be simulated in the biological culture. The situation of flow in the body, in addition, the limiting member can be replaced with different shapes, thereby simulating different biological environments, and detecting the influence of fluid pressure, fluid shear force or fluid pulse on the cells on at least one biological culture material, High versatility, the closed space formed in the reactor module can avoid external interference and further improve the simulation accuracy.

Before the present invention is described in detail, it should be noted that in the following description, similar elements are denoted by the same numerals.

Referring to Fig. 1, it is a first embodiment of the biomechanical testing system of the present invention, comprising a reactor module 1, a storage tank 2 containing liquid and connected to the reactor module 1, an air pressure connected to the storage tank 2 A source 3 , a gas supply source 4 connected to the reactor module 1 , a buffer tank 5 connected to the reactor module 1 , and a liquid discharge pipeline 6 connected to the reactor module 1 .

Referring to Fig. 2 to Fig. 5, define mutually perpendicular a first horizontal direction A, a second horizontal direction B, and a vertical direction C, this reactor module 1 comprises a connecting this buffer tank 5 (see Fig. 1) An upper formwork 11, a lower formwork 12 arranged below the upper formwork 11 along the vertical direction C and connected to the drain pipeline 6 (see Figure 1), a lower formwork 12 arranged on the upper formwork 11 and the upper formwork 11 along the vertical direction C The positioning plate 13 between the lower formwork 12, two sealing gaskets 14 respectively located between the upper and lower formwork 12 and the positioning plate 13 along the vertical direction C, one surrounded by the positioning plate 13 and along the vertical direction C A limiting member 15 located between the upper and lower templates 12 , and a biological culture material 16 against the limiting member 15 . The upper template 11 , the lower template 12 , and the positioning plate 13 cooperate to define a closed space 17 for accommodating the limiting member 15 and the biological cultivation material 16 . The sealing gaskets 14 are in the form of annular sheets surrounding the closed space 17 .

The upper template 11 has two electrode groups 111 spaced apart from each other along the second horizontal direction B and each extending downward along the vertical direction C in the enclosed space 17 . Each electrode group 111 is generally in the shape of a tooth comb as shown in Figure 3, that is, each electrode group 111 has a plurality of electrodes arranged at intervals along the first horizontal direction A, and a plurality of supply electrodes are arranged on both sides of the limiting member 15. The slots (see Fig. 2) that these electrode groups 111 pass through allow it to pass through the stopper 15 and contact the biological cultivation material 16, thereby pressing, inserting or passing through the biological cultivation material 16 as shown in Fig. 5 . material 16, and perform electrical stimulation simulation on the cells on the biological culture material 16 through external power supply (for example, for muscle cells, heart cells or nerve cells), the appearance of the tooth comb plus the configuration of inserting the biological culture material 16 It can make electrical stimulation more uniform and fast. It should be noted that these electrode groups 111 are components that are selectively installed. In this first embodiment, electrodes of other shapes can also be installed, or no electrodes can be installed. Therefore, It should not be limited by this.

The lower formwork 12 is recessed along the vertical direction C to form a receiving groove 121 communicating with the closed space 17 , and the receiving groove 121 is used for connecting with the drainage pipeline 6 . In addition, the lower template 12 can also have a heating function, so that the enclosed space 17 can be heated to a set temperature to simulate the temperature inside the human body. The gaskets 14 are made of silicone and are used to ensure the sealing of the enclosed space 17 . The upper mold plate 11 , the positioning plate 13 , the lower mold plate 12 , and the sealing gaskets 14 are fixed together vertically and overlappingly through screws.

Referring to Figure 2, Figure 5, and Figure 6, the limiting member 15 has a plate body portion 151 extending along the first horizontal direction A, and two side edges protruding from the plate body portion 151 along the vertical direction C part 152, and two protruding wall parts 153 protruding from the side edge parts 152 along the second horizontal direction B. The side edge portions 152 are spaced apart from each other along the second horizontal direction B and extend along the first horizontal direction A respectively. The plate body portion 151, the side edge portions 152 and the convex wall portions 153 cooperate to define a flow channel 154 extending along the first horizontal direction A, and the front and rear ends of the flow channel 154 are connected to the closed space 17 are connected, and the side of the biological culture material 16 used for culturing cells faces the flow channel 154 . It should be noted that, when the limiting member 15 is placed in the closed space 17, the side edges 152 are set in such a way that the side edges 152 face downward, but in order to illustrate the structure of the limiting member 15, FIG. 6 is Presented upside down. The biological cultivation material 16 is adjacent to the side edges 152 and located below the limiting member 15 . In this first embodiment, the biological culture material 16 can be a silica gel film for culturing cells in an attached manner, a glass slide capable of cultivating cells and extramatrix, or a biomedical material suitable for cells and soft materials, and does not use This is the limit.

Referring to Fig. 6, Fig. 7, and Fig. 8, in the first embodiment, each convex wall portion 153 is semicircular and opposite to each other, so that the flow channel 154 presents a slit shape to simulate blood vessel blockage or other In order to simulate different degrees of blockage in the narrow passage environment in the living body, the convex wall portions 153 can be designed in different sizes as shown in FIGS. 6 to 8 according to requirements. Referring to Fig. 9, Fig. 10, and Fig. 11, the limiting member 15 may not be provided with the convex wall parts 153, but has a protruding part along the vertical direction C on the plate body part 151 and located in the flow Module section 155 in lane 154. The module part 155 can form a bifurcation on the flow channel 154, thereby simulating the bifurcation of blood vessels or other biological environments. The protruding angles of the bifurcation can also be at different angles as shown in Figures 9 to 11 to simulate different types of blood vessel bifurcations. Therefore, the operator can disassemble and replace the suitable limiter according to the biological environment to be simulated 15. To establish flow channels 154 with different boundary conditions according to requirements, which is highly versatile and facilitates modularization. In the first embodiment, the material of the module part 155 can be an acrylic block.

Referring to Fig. 12 and Fig. 13, the limiting plate 15 can also be arranged in reverse (so that the electrode groups 111 do not need to pass through the limiting plate 15), if the biological culture material 16 is to adopt the plane in Fig. 2 During structural design, the biological culture material 16 is placed upside down and upside down. When the aforementioned biological culture material 16 is a plurality of bulk biomedical materials as shown in FIG. It is columnar, so as shown in Figure 12, the biological cultivation materials 16 can be fixed on the limiting plate 15 at intervals along the first horizontal direction A, expanding the applicable range of the biological cultivation materials 16, and increasing Diversification of analog detection.

Referring again to Fig. 1, Fig. 2, and Fig. 4, the storage tank 2 contains liquids such as culture fluid, and can be forced by the gas input from the air pressure source 3, so that the liquid passes through the controlled and opened silicon rubber pipeline, and passes through the The positioning plate 13 is sent into the closed space 17 and enters into the flow channel 154 . The air supply source 4 is connected to the silicone pipeline of the storage barrel 2 through the silicone pipeline, thereby communicating with the closed space 17 . The buffer tank 5 is connected to the upper formwork 11 through a silicone pipeline. When the liquid in the closed space 17 is too much, the excess liquid will directly overflow into the buffer tank 5 . The drain pipeline 6 is connected to the lower template 12 and connected to the receiving tank 121, and is also connected to the pipeline of the buffer tank 5 to communicate with the upper template 11. These two places can be opened by controlling to discharge the liquid to the External recovery (detailed steps in this part will be described later). It should be noted that although the storage barrel 2, the air supply source 4, the buffer tank 5, and the liquid discharge pipeline 6 are all connected to the closed space 17, the storage barrel 2, the air supply source 4, and Solenoid valves are arranged on the pipelines of the liquid discharge pipeline 6 , and the buffer tank 5 is in a closed environment, so the closedness of the closed space 17 can be maintained.

Referring to Fig. 1, Fig. 2, and Fig. 6, the first embodiment has multiple usage modes: when the flow channel 154 is to be filled with culture solution or to replace the culture solution for simulation, the main controller D transmits the analog signal Control the programmable air pressure controller, open the solenoid valve and drive the air pressure source 3 to send gas into the storage tank 2, and the culture solution in the storage tank 2 can be sent into the closed space 17 through the air pressure (the storage tank 2 The electromagnetic valve E on the pipeline will open), and inject into the flow channel 154 until it is full, and cooperate with the setting of the convex wall parts 153 or the module part 155 (see Figure 8) to create different flow channel 154 environments ( Such as vascular blockage or vascular bifurcation), and under these different biological structures, perform cyclic fluid shear simulation, stable fluid pressure simulation, uniform and rapid electrical stimulation simulation, etc. During the aforementioned liquid filling or liquid changing process, the solenoid valve F connecting the drain line 6 to the buffer tank 5 can be opened, so that the culture medium can be discharged through the drain line 6 . Referring to Fig. 1, Fig. 5, and Fig. 14, the air pressure source 3 can also input the culture solution in the storage tank 2 into the flow channel 154 through the pulse mode, so as to achieve the effect of fluid pulse input, and can pass through the pressure gauge G, etc. The instrument checks the condition of the pulse, so that fluid pulse stimulation simulation can be carried out. During the process of pulse input, the culture solution temporarily overflowed due to the pulse will first flow into the buffer tank 5 for temporary buffering, but it is also possible not to set this The buffer groove 5, but let the pressure remain in the limiting member 15 for extrusion.

Referring to Fig. 1, Fig. 5, and Fig. 15, when the liquid in the closed space 17 and the flow channel 154 is to be emptied, the air pressure source 3 can be closed and the gas supply source 4 can be opened, so that the gas directly enters the closed space 17, and through the air pressure, the culture solution is discharged from the drain line 6 (at this time, the electromagnetic valve H of the drain line 6 needs to be opened), so that the effect of emptying the liquid can be achieved. Referring to Fig. 1, Fig. 5, and Fig. 16, the biomechanical testing system may also include a circulation pipeline 71 connecting the storage tank 2 and the reactor module 1 and being provided with a solenoid valve 1, and a circulation line 71 arranged in the circulation Pump 72 on pipeline 71. This disposition mode can be after the culture liquid is filled with this channel 154, through this pump 72, the culture liquid is drawn out from this circulation pipeline 71 (the solenoid valve 1 of this circulation pipeline 71 is opened), and the culture fluid is returned to this storage. Create a fluid shear cycle in the barrel 2 to achieve a variety of conditions and environmental simulations.

Referring to Fig. 17, Fig. 18, and Fig. 19, it is a second embodiment of the biomechanical testing system of the present invention, the second embodiment is substantially the same as the first embodiment, the difference lies in: the reactor mold The limiting member 15 of group 1 has two receiving portions 156 inserted on the positioning plate 13 . The biological culture material 16 is tubular and the enclosed space 17 is divided into an inner flow channel 154 and an outer ring groove 157 surrounding the flow channel 154 . Both ends of the biological culture material 16 are respectively disposed on the receiving portions 156 . The storage barrel 2 includes a barrel body 21 filled with liquid and connected to the air pressure source 3 , a first pipeline 22 connected to the barrel body 21 and connected to the flow channel 154 through the positioning plate 13 , and a barrel body 21 connected to And communicate with the second pipeline 23 of the outer ring groove 157 . The first pipeline 22 is connected to one of the receiving portions 156 through the positioning plate 13 , and the drain pipeline 6 is connected to the other receiving portion 156 through the positioning plate 13 , and is also connected to the outer ring groove 157 . The buffer tank 5 is not provided in the second embodiment.

The mode of operation of the second embodiment is as follows: the air pressure source 3 provides gas to the barrel body 21, so that the culture solution in the barrel body 21 is transferred from the first pipeline 22 (the electromagnetic of the second pipeline 23) through the air pressure. Valve J is closed), and then the culture solution enters the flow channel 154. In addition, the culture solution can also be discharged by opening the electromagnetic valve K of the drain line 6. When it is desired to perform a pulse reaction, only the first one can be opened. The solenoid valve L of the pipeline 22 is used to control the programmable air pressure controller through an analog signal through the main controller D, so that the air pressure source 3 intermittently inputs gas to generate a pulse response in the flow channel 154 . 18 and 20, the user can also close the first pipeline 22 and open the second pipeline 23, so that the culture solution can enter the outer ring groove 157 from the second pipeline 23, and pass through Open another electromagnetic valve M of the liquid discharge pipeline 6 to discharge the culture solution, so as to achieve another effect of environment simulation.

Referring to FIG. 18 and FIG. 21 , the second embodiment also includes two circulation pipelines 71 that are connected to the storage tank 2 and the drain pipeline 6 and can be opened under control, and one of the circulation pipelines 71 communicates with the flow channel 154 , another circulation pipeline 71 communicates with the outer ring groove 157 and the second pipeline 23 , and each of the circulation pipelines 71 is provided with a pump 72 . When the circulation line 71 connected to the flow channel 154 is opened, the culture solution can be sucked by the pump 72 of the circulation line 71 after entering the flow channel 154 (corresponding to the opening of the solenoid valve N) to follow the flow path 154. The circulation pipeline 71 returns to the barrel body 21 to achieve the simulation effect of circulation in the pipe. Referring to Fig. 18 and Fig. 22, when the outer ring groove 157 is filled with culture solution, most of the electromagnetic valves can be closed, leaving only the electromagnetic valve J of the second pipeline 23 and the circulation pipe connected to the outer ring groove 157 The electromagnetic valve O of the road 71, through the pump 72, the culture solution is in the second pipeline 23, the outer ring groove 157, the liquid discharge pipeline 6 (it will not be discharged because the electromagnetic valve M is closed), and the circulation Circulation among pipelines 71 achieves the simulation effect of external circulation.

Referring to Fig. 18 and Fig. 23, when the culture solution in the flow channel 154 is to be emptied, the air pressure source 3 can be closed, and the gas supply source 4 can be opened, so that the gas of the gas supply source 4 can be directly fed from the The first pipeline 22 is sent into the flow channel 154 to discharge the culture fluid from the drain pipeline 6 to achieve the effect of emptying the liquid in the pipe. A gas supply source (not shown) connected to the second pipeline 23 is provided. In the second embodiment, a tubular biomedical material can be used as the biological culture material 16 to increase the diversity of the simulated environment.

To sum up, the present invention can replace the biological culture material 16 of different shapes according to the needs, and only need to replace the limiting plate 15 to create flow channels 154 with different structural features such as narrowness and branching, and to create circulation Fluid shear force simulation, stable fluid pressure simulation, fluid pulse stimulation simulation, and electrical stimulation simulation and many other bioenvironment simulations, so as to achieve a highly versatile detection effect, so the purpose of the present invention can indeed be achieved.

But what is described above is only an embodiment of the present invention, and should not limit the scope of the present invention. All simple equivalent changes and modifications made according to the patent scope of the present invention and the content of the patent specification are still within the scope of the present invention. Within the scope covered by the patent of the present invention.

1········ Reactor Module 11······ Upper template 111····· Electrode group 12······ Lower template 121····· Receiving groove 13······ Positioning plate 14······ Gasket 15······ Limiting parts 151····· Board body 152····· side edge 153····· convex wall 154····· Runner 155····· Module Department 156····· Receiving Department 157····· Outer ring groove 16······ Biological culture materials 17······ Confined Space 2········ Storage Bucket 21······ Barrel 22······ First pipeline 23······ Second pipeline 3······················································ 4·················································· 5········· buffer tank 6········ Drain Line 71······Circulation pipeline 72······ pump A·······First Horizontal Direction B·······Second Horizontal Direction C······· Vertical direction D······· Master Controller E········ Solenoid valve F········ Solenoid valve G······· Pressure Gauge H~O···· Solenoid valve

Other features and effects of the present invention will be clearly presented in the implementation manner with reference to the drawings, wherein: Fig. 1 is a schematic diagram illustrating a first embodiment of a biomechanical testing system of the present invention; FIG. 2 is an exploded perspective view illustrating a reactor module of the first embodiment; Fig. 3 is a perspective view illustrating two electrode groups of the first embodiment; Fig. 4 is a side view sectional view illustrating the side view sectional appearance after the assembly of Fig. 2; Fig. 5 is a front sectional view illustrating the front sectional appearance of Fig. 4; Fig. 6 to Fig. 11 are all three-dimensional views, illustrate the different forms of one limit piece of this reactor module; Fig. 12 is a side view sectional view illustrating another aspect and setting method of the limiting member; Fig. 13 is a perspective view to assist in explaining the three-dimensional aspect of Fig. 12; FIG. 14 and FIG. 15 are schematic diagrams illustrating different simulation actions of the first embodiment; Fig. 16 is a schematic diagram illustrating another aspect of the first embodiment and its simulation action; 17 is a front sectional view illustrating a second embodiment of the biomechanical testing system of the present invention; Fig. 18 is a side view sectional view illustrating the side view sectional form of Fig. 17; Figure 19 is a schematic diagram illustrating the configuration of the second embodiment; and 20 to 23 are schematic diagrams illustrating different simulation actions of the second embodiment.

1········ Reactor Module 11······ Upper template 12······ Lower template 121····· Receiving groove 13······ Positioning plate 14······ Gasket 15······ Limiting parts 151····· Board body 152····· side edge 154····· Runner 16······ Biological culture materials 17······ Confined Space A·······First Horizontal Direction B·······Second Horizontal Direction C······· Vertical direction

Claims (23)

A biomechanical testing system comprising: A reactor module, including an upper template, a lower template arranged below the upper template, a positioning plate arranged between the upper template and the lower template, a positioning plate surrounded by the positioning plate and located on the upper template and The limiter between the lower templates, and at least one pair of biological culture materials that should be set on the limiter, the upper template, the lower template, and the positioning plate cooperate to define a space for accommodating the limiter and the confined space of the at least one biological culture material; a storage tank containing liquid and connected to the reactor module, the storage tank can be controlled to supply liquid to the confined space; and An air pressure source is connected to the storage tank, and the air pressure source can be controlled to provide gas to the storage tank, so that the liquid in the storage tank enters the closed space through the air pressure and flows through the at least one biological culture material. The biomechanical testing system as claimed in item 1, wherein the limiting member defines a pair of flow passages corresponding to at least one biological culture material and communicating with the enclosed space, and the flow passages can allow the liquid in the storage barrel to flow through . The biomechanical testing system according to claim 2 further includes a buffer tank connected to the reactor module, and the buffer tank receives excess liquid overflowing from the flow channel. The biomechanical testing system as described in claim 3, defining a first horizontal direction, a second horizontal direction, and a vertical direction perpendicular to each other, wherein the limiting member has a length extending along the first horizontal direction a plate body, and two side edge portions protruding from the plate body along the vertical direction, the side edge portions are spaced apart from each other along the second horizontal direction and each extend along the first horizontal direction. The biomechanical testing system as claimed in claim 4, wherein the limiting member further has a plurality of convex wall portions protruding from the side edge portions along the second horizontal direction. The biomechanical testing system according to claim 4, wherein the limiting member further has at least one module part arranged on the plate body part and located in the flow channel, and the module part is a polygonal column. The biomechanical testing system according to claim 5 or 6, wherein the liquid in the storage barrel enters the confined space through the positioning plate, and the flow channel communicates with the confined space, and the excess liquid overflowing from the flow channel passes through the upper template Drain to the buffer tank. The biomechanical testing system according to claim 7, further comprising a drain line connected to the reactor module and opened to allow the liquid in the channel to drain out. The biomechanical testing system according to claim 8, further comprising a circulation pipeline connecting the storage tank and the reactor module, and at least one pump arranged on the circulation pipeline. The biomechanical testing system as claimed in claim 1, wherein the reactor module includes a tubular biological culture material positioned by the limiting member, and the biological culture material divides the enclosed space into a flow channel and a surrounding The outer annular groove of the flow channel, the storage barrel includes a barrel body containing liquid and connected to the air pressure source, a first pipeline connected to the barrel body and communicated with the flow channel, and a first pipeline connected to the barrel body and communicated with the outer The second line of the ring groove. The biomechanical testing system as described in claim 10, further comprising a drain pipe connected to the reactor module and opened to allow the liquid of the flow channel to discharge, the limiting member has two inserts in the position and positioning the receiving part of the biological culture material, the first pipeline is connected to one of the receiving parts through the positioning plate, the drainage pipeline is connected to the other receiving part through the positioning plate, and the outer ring groove is also connected to the Drain line connection. The biomechanical testing system as described in claim 11, further comprising two circulating pipelines connected to the storage barrel and the drain pipeline and controlled to be opened, wherein one circulating pipeline communicates with the flow channel, and the other circulating pipeline A road connects the outer ring groove and the second pipeline, and each of the circulation pipelines is provided with a pump. The biomechanical testing system according to claim 1, further comprising a gas supply source capable of supplying gas to the reactor module in a controlled manner. The biomechanical testing system as claimed in item 1, wherein the upper template has two electrode groups extending downwards in the enclosed space and each in the shape of a toothed comb, and the electrode groups are pressed, inserted or passed through the at least 1. Biological culture materials. The biomechanical testing system as claimed in item 1, wherein the lower template can heat the enclosed space. The biomechanical testing system according to claim 1 further comprises two ring-shaped sealing gaskets sandwiched between the upper template, the positioning plate, and the lower template. A reactor module comprising: a template; The lower template is set under the upper template; A positioning plate is arranged between the upper formwork and the lower formwork, and the positioning plate, the upper formwork, and the lower formwork cooperate to define a closed space; A limiting part is arranged in the closed space, the limiting part is surrounded by the positioning plate and is located between the upper formwork and the lower formwork; and At least one biological culture material is located in the closed space and arranged corresponding to the limiting member. The reactor module as described in claim item 17 defines a first horizontal direction, a second horizontal direction, and a vertical direction that are perpendicular to each other, wherein the limiting member defines a flow channel that communicates with the closed space , and includes a body portion extending along the first horizontal direction, and two side edge portions protruding from the body portion along the vertical direction, the side edge portions are spaced from each other along the second horizontal direction and Each extends along the first horizontal direction. The reactor module as claimed in claim 18, wherein the limiting member further includes a plurality of convex wall portions protruding oppositely from the side edge portions along the second horizontal direction. The reactor module as claimed in claim 18, wherein the limiting member further includes at least one module part disposed on the plate body part and located in the flow channel, and the module part is a polygonal column. The reactor module as described in claim 17, comprising a biological cultivation material in a tubular shape and positioned by the limiting member, the biological cultivation material divides the enclosed space into a flow channel and an outer ring groove surrounding the flow channel . The reactor module as claimed in item 17, wherein the upper template has two electrode groups extending downwards in the enclosed space and each in the shape of a toothed comb, and the electrode groups are pressed, inserted or passed through the at least 1. Biological culture materials. The reactor module as claimed in item 17, wherein the lower template can heat the enclosed space.

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