TWI742905B - Nucleic acid extracting device - Google Patents

Abstract

A nucleic acid extracting device includes a reagent containing unit, a mixing unit and a flow channel unit. The reagent containing unit is adapted to contain a specimen, a magnetic bead and a reagent for extracting. The mixing unit includes a mixing chamber and a stirring assembly. The mixing chamber includes a chamber portion and a tube portion, and the stirring assembly includes a main portion and an extending portion. The main portion is located in the chamber portion, the tube portion is connected to the chamber portion and the extending portion is connected to the main portion and extended into the tube portion. The extending portion and an inner wall of the tube portion have first and second gaps therebetween respectively along first and second directions of the tube portion, and the first gap is less than the second gap. The flow channel unit is connected between the reagent containing unit and the mixing unit. The specimen, the magnetic bead and the reagent are adapted to flow from the reagent containing unit to the mixing unit through the flow channel and to be stirred and mixed by the stirring assembly.

Description

Nucleic acid extraction device

This creation is about an extraction device, and especially about a nucleic acid extraction device.

Nucleic acid analysis is an indispensable method for the research or detection of genetics, molecular biology, or animal and plant diseases. Therefore, nucleic acid separation and extraction related technologies have developed rapidly in recent years. A method of nucleic acid extraction in which the sample, magnetic beads, and various reagents required for nucleic acid extraction are mixed in a mixing tank according to the established process and sequence, so that the nucleic acid of the sample is first bound to the magnetic beads and then separated from the magnetic beads Out of nucleic acid. Nucleic acid analysis focuses on timeliness. For example, for emerging infectious diseases, the faster the nucleic acid analysis of bacteria or viruses can be completed, the faster the corresponding vaccine can be developed. However, the general mixing device cannot efficiently mix the magnetic beads and the reagents, which greatly increases the time required for nucleic acid extraction and analysis.

This creation provides a nucleic acid extraction device that can efficiently mix magnetic beads and reagents.

The nucleic acid extraction device of this invention includes a reagent containing unit, a mixing unit and a flow channel unit. The reagent containing unit is suitable for containing at least one sample, at least one magnetic bead and at least one extraction reagent. The mixing unit includes a mixing tank and a stirring component. The mixing tank includes a tank part and a pipe part, and the stirring assembly includes a main body part and an extension part. The main body part is located in the groove part, the tube part is connected to the groove part, and the extension part is connected to the main body part and extends into the tube part. The extension portion and the inner wall of the pipe portion have a first gap in a first direction of the pipe portion, and the extension portion and the inner wall of the pipe portion have a second gap in a second direction of the pipe portion, and the first gap is smaller than The second gap. The flow channel unit is connected between the reagent containing unit and the mixing unit. The sample, the magnetic beads, and the extraction reagent are adapted to be moved from the reagent storage unit to the mixing tank through the flow channel unit, and are stirred and mixed by the stirring component.

In an embodiment of the present creation, the inner width of the aforementioned tube portion is smaller than the inner width of the groove portion.

In an embodiment of the present invention, the inner width of at least a part of the above-mentioned groove portion is tapered from top to bottom, and the main body portion is arranged in the corresponding tapered position of the groove portion to have an airfoil shape.

In an embodiment of the present creation, the width of the aforementioned extension part is smaller than the width of the main body part.

In an embodiment of the present creation, the above-mentioned pipe part is connected between the flow channel unit and the groove part.

In an embodiment of the present creation, the above-mentioned extension has a first length in the first direction and a second length in the second direction, and the first length is greater than the second length.

In an embodiment of the present creation, the above-mentioned pipe part has a cylindrical pipe, and the extension part is located in the cylindrical pipe and has a rectangular cross section.

In an embodiment of the present invention, the aforementioned mixing unit includes an actuator, which is connected to the stirring assembly and is adapted to drive the stirring assembly to rotate.

In an embodiment of the present invention, the aforementioned nucleic acid extraction device further includes a heating device, wherein the heating device is arranged beside the mixing tank and is suitable for heating the mixing tank.

In an embodiment of the present invention, the aforementioned nucleic acid extraction device further includes a magnetic attraction device, wherein the magnetic attraction device is movably disposed outside the tube portion, and is adapted to prevent the magnetic beads from moving away from the tube portion by magnetic attraction.

In an embodiment of the present invention, the aforementioned nucleic acid extraction device further includes a pump, wherein the pump is connected to the mixing tank and is adapted to drive the specimen, magnetic beads, and extraction reagent to move between the reagent containing unit and the mixing tank.

In an embodiment of the invention, the above-mentioned reagent containing unit has a plurality of reagent tanks, and these reagent tanks are suitable for accommodating samples, magnetic beads, and extraction reagents, respectively.

In an embodiment of the present invention, the aforementioned extraction reagents include a lysis solution, a bonding solution, a washing solution, and an eluent. Some of these reagent tanks are suitable for holding the lysis solution, the bonding solution, the washing solution, and the washing solution, respectively. Deliquid.

In an embodiment of the present invention, the reagent tank corresponding to the magnetic beads has an inlet and an outlet, and the position of the inlet is higher than the outlet.

In an embodiment of the present invention, there is a guide surface in the reagent tank corresponding to the magnetic beads, and the guide surface extends obliquely from the entrance to the exit.

In an embodiment of the present creation, the bottom of the reagent tank corresponding to the magnetic beads has a recess, the outlet is located in the recess, and the recess is suitable for accommodating the magnetic beads.

In an embodiment of the present creation, the above-mentioned reagent containing unit includes a containing structure, these reagent tanks are formed in the containing structure, and the bottom of the containing structure has a plurality of channels connected to the reagent tanks respectively, and each reagent tank penetrates correspondingly. The channel is connected to the flow channel unit.

In an embodiment of the present creation, each of the above-mentioned channels is a capillary circuit.

In an embodiment of the present creation, the above-mentioned reagent containing unit further includes a plurality of elastic sealing elements and a bottom plate. The elastic sealing elements are arranged at the bottom of the containing structure and respectively correspond to the channels. The bottom plate is assembled at the bottom of the containing structure and Each elastic sealing element is confined between the containing structure and the flow channel unit. Each elastic sealing element has a top surface and a bottom surface opposite to each other, and has a through hole. The through hole extends from the top surface to the bottom surface, and the elastic sealing element is arranged in the bottom plate. , And the top surface and the bottom surface respectively contact the containing structure and the flow channel unit, and the through holes are connected to the corresponding channels.

In an embodiment of the present creation, the above-mentioned flow channel unit has a flow channel, one end of the flow channel is connected to the mixing tank, and the reagent containing unit is rotatably arranged on the flow channel unit and is suitable for rotation so that any reagent tank corresponds to At the other end of the runner.

Based on the above, in addition to the existing tank, the mixing tank of the present invention has a pipe part extending from the tank part, and the stirring assembly correspondingly has an extension part extending into the pipe part. In addition, there are first gaps and second gaps of different sizes between the extension portion of the stirring assembly and the inner wall of the pipe portion, that is, the size of the gap between the extension portion and the inner wall of the pipe portion is non-uniform. The uneven gap between the extension part of the stirring assembly and the pipe part makes the liquid produce uneven capillary force. When the pump sucks in from the upper end of the mixing tank, the liquid will flow up and down again and again in the pipe part, which can be attached to the pipe wall On the magnetic beads remelted.

Figure 1 is a perspective view of a nucleic acid extraction device according to an embodiment of the invention. Fig. 2 is an exploded view of the nucleic acid extraction device of Fig. 1. Fig. 3 is a cross-sectional view of the nucleic acid extraction device of Fig. 1. Please refer to FIGS. 1 to 3, the nucleic acid extraction device 100 of this embodiment includes a reagent containing unit 110, a mixing unit 120 and a flow channel unit 130. The flow channel unit 130 is composed of, for example, an upper plate body 132 and a lower plate body 134 stacked together, and is connected between the reagent containing unit 110 and the mixing unit 120. The reagent accommodating unit 110 has a plurality of reagent tanks 110a to 110g, and these reagent tanks 110a to 110g are suitable for accommodating samples, a plurality of magnetic beads, and various extraction reagents, respectively. The mixing unit 120 includes a mixing tank 122 and a stirring assembly 124. The sample, magnetic beads, and extraction reagent are adapted to move from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 according to the established process and sequence, and are stirred and mixed by the stirring assembly 124, so that the nucleic acid of the sample is first bound to Magnetic beads, and then separate nucleic acid from the magnetic beads.

In this embodiment, the mixing tank 122 includes a groove portion 1221 and a pipe portion 1222. The pipe portion 1222 is connected between the flow channel unit 130 and the groove portion 1221. The inner width of the pipe portion 1222 is smaller than the inner width of the groove portion 1221. It should be noted that, in this embodiment, the cross section of the tube portion 1222 and the groove portion 1221 is circular, so the aforementioned inner width refers to the inner diameter. The stirring assembly 124 includes a main body portion 1241 and an extension portion 1242, and the width of the extension portion 1242 is smaller than the width of the main body portion 1241. The main body portion 1241 is located in the groove portion 1221, and the extension portion 1242 is connected to the main body portion 1241 and extends into the tube portion 1222. When the stirring assembly 124 is driven and actuated, the main body portion 1241 stirs the sample, magnetic beads, and/or extraction reagent in the tank portion 1221, and the extension portion 1242 stirs the sample, magnetic beads, and/or the sample in the tube portion 1222. Or the extraction reagent is stirred.

Fig. 4 is a cross-sectional view of the mixing unit of Fig. 1 along the line I-I. Please refer to FIG. 4, furthermore, the extension portion 1242 and the inner wall of the tube portion 1222 of this embodiment are in a first direction RD1 of the tube portion 1222 (that is, a radial direction perpendicular to the axial direction AD of the tube portion 1222). There is a first gap G1, and the extension portion 1242 and the inner wall of the pipe portion 1222 have a second gap in a second direction RD2 of the pipe portion 1222 (that is, the other radial direction perpendicular to the axial direction AD of the pipe portion 1222) G2, the first gap G1 is smaller than the second gap G2. That is, the size of the gap between the extension portion 1242 and the inner wall of the tube portion 1222 is not uniform. As a result, the capillary force between the extension portion 1242 and the inner wall of the pipe portion 1222 is uneven, so that when the reagent flows in the mixing tank 122 due to the driving of the pump 160 and/or the stirring of the stirring assembly 124, the inside of the pipe portion 1222 The reagents at different positions have different flow rates, so that bubbles are easily formed in the tube portion 1222, and the mixing of the magnetic beads and the reagent can be accelerated by the stirring of the stirring assembly 124 and the disturbance of the bubbles.

Specifically, the pipe portion 1222 of this embodiment has a cylindrical pipe 1222a, and the extension portion 1242 is located in the cylindrical pipe 1222a. The extension portion 1242 has a rectangular cross section, so that the extension portion 1242 has a first length L in the first direction RD1 and a second length W in the second direction RD2, and the first length L is greater than the second length W. Thereby, the size of the gap between the extension portion 1242 and the inner wall of the pipe portion 1222 can be made non-uniform as described above. In other embodiments, the pipe portion 1222 may have a pipe with other cross-sectional shapes, and the extension portion 1242 may have a cross-section of other shapes, so that the gap between the extension portion 1242 and the inner wall of the pipe portion 1222 is non-uniform, for example The pipe portion 1222 has a rectangular pipe, and the extension portion 1242 is located in the rectangular pipe and has a circular cross-section, which can also produce a non-uniform gap, and this creation does not limit its specific shape.

Please refer to FIG. 3, the mixing unit 120 of this embodiment includes an actuator 126 and a cover 128. The cover 128 covers the tank portion 1221 of the mixing tank 122, and the stirring assembly 124 further includes a connecting portion 1243. The connecting portion 1243 can pass through the cover 128 and partially protrude to the outside of the mixing tank 122 and is connected to the actuator 126. The actuator 126 is, for example, a motor and is adapted to drive the stirring assembly 124 to rotate to perform the stirring. In other embodiments, the actuator 126 may be other types of driving devices, which is not limited in the present invention. In addition, the mixing unit 120 may not include the actuator 126, but an actuator included in other external devices to drive the stirring assembly 124 to act.

As shown in FIG. 3, the nucleic acid extraction device 100 of this embodiment further includes a heating device 140, a magnetic attraction device 150 and a pump 160. The heating device 140 is arranged next to the mixing tank 122 and is suitable for heating the mixing tank 122 to accelerate the reaction rate of the sample and the reagent. The magnetic attraction device 150 is movably disposed outside the tube portion 1222 and is suitable for restricting the position of the magnetic beads by magnetic attraction, so as to prevent the magnetic beads from moving away from the tube portion 1222 unexpectedly due to the flow of the reagent. The pump 160 is connected to the mixing tank 122 and is suitable for driving the specimen, magnetic beads and/or extraction reagent to move between the reagent containing unit 110 and the mixing tank 122. The heating device 140 and the magnetic attraction device 150 can be respectively arranged at positions adjacent to the groove portion 1221 and the tube portion 1222 as shown in FIG. The suction device 150 is heated and magnetically attracted by a heating device and a magnetic suction device included in other external equipment. When the heating device 140 and/or the magnetic attraction device 150 is not required to perform heating/magnetic attraction, the heating device 140 and/or the magnetic attraction device 150 can be driven to move away from the mixing tank 122 or the heating device 140 and/or magnetic attraction can be turned off装置150。 Device 150. In addition, the nucleic acid extraction device 100 may not include the pump 160, but a pump included in another external device to drive the specimen, magnetic beads, and/or extraction reagent to move.

In this embodiment, the extraction reagent may include a lysis buffer, a binding buffer, a washing buffer, and an elution buffer. The reagent tank 110a shown in FIG. 1 is used to contain the specimen, the reagent tank 110b is used to contain the lysis solution, the reagent tank 110c is used to contain the bonding solution, the reagent tank 110d is used to contain magnetic beads, and the reagent tanks 110e and 110f are used to contain washing. The reagent tank 110g is used to hold the eluent.

The specific operation flow of the nucleic acid extraction device 100 of this embodiment will be described below. First, the sample in the reagent tank 110 a and the lysate in the reagent tank 110 b are moved from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 by the driving of the pump 160. The heating device 140 heats the mixing tank 122 and the stirring component 124 agitates the sample and the lysis solution in the mixing tank 122, so that the cell membrane of the sample is destroyed by the lysis solution to precipitate nucleic acid. Then, the bonding liquid in the reagent tank 110 c and the magnetic beads in the reagent tank 110 d are sequentially moved from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 by the driving of the pump 160. The heating device 140 heats the mixing tank 122, and the stirring component 124 stirs the sample, the magnetic beads, and the bonding liquid in the mixing tank 122, so that the nucleic acid of the sample is bonded to the magnetic beads by the bonding liquid. Then, the magnetic attraction force of the magnetic attraction device 150 prevents the magnetic beads from moving, and the pump 160 drives the waste liquid generated by the reaction between the sample and the reagent in the mixing tank 122 to pass through the flow channel unit 130 to be discharged to the reagent containing unit 110, wherein the reagent contains The unit 110 may include a waste liquid tank or use an existing reagent tank to contain the waste liquid.

Next, the washing liquid in the reagent tank 110 e is moved from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 by the driving of the pump 160. The stirring component 124 stirs the magnetic beads and the washing liquid in the mixing tank 122 to wash the magnetic beads for the first time with the washing liquid. The magnetic attraction force of the magnetic attraction device 150 prevents the magnetic beads from moving, and uses the pump 160 to drive the waste liquid generated in the mixing tank 122 after the first washing through the flow channel unit 130 to be discharged to the reagent containing unit 110, wherein the reagent contains The unit 110 may include a waste liquid tank or use an existing reagent tank to contain the waste liquid. Then, the washing liquid in the reagent tank 110 f is moved from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 by the driving of the pump 160. The stirring component 124 stirs the magnetic beads and the washing liquid in the mixing tank 122 to wash the magnetic beads for the second time with the washing liquid. The magnetic attraction force of the magnetic attraction device 150 prevents the magnetic beads from moving, and uses the pump 160 to drive the waste liquid generated from the second washing in the mixing tank 122 through the flow channel unit 130 to be discharged to the reagent containing unit 110, wherein the reagent contains The unit 110 may include a waste liquid tank or use an existing reagent tank to contain the waste liquid. The eluate in the reagent tank 110 g is moved from the reagent storage unit 110 through the flow channel unit 130 to the mixing tank 122 by the driving of the pump 160. The stirring component 124 stirs the magnetic beads and the eluent in the mixing tank 122 to separate the nucleic acid from the magnetic beads by the eluent, thereby extracting the nucleic acid.

In different steps of the foregoing operation flow, the amount of reagent in the mixing tank 122 may be different. In order to make the mixing tank 122 suitable for various different amounts of reagents, the tank part 1221 at the connection end of the pipe part 1222 can be designed as a funnel shape as shown in FIGS. 1 to 3, and the tank part 1221 is partially The inner width gradually shrinks from top to bottom. Thereby, when the amount of reagent in the mixing tank 122 is large, its section with a larger inner width provides enough space to accommodate the reagent, and when the amount of reagent in the mixing tank 122 is small, it has a larger amount of reagent. The small inner width section prevents the reagent from being excessively dispersed in the horizontal direction due to the excessively large inner width of the mixing tank 122, so as to reduce the chance of remaining in the dead corner of the tank 1221. Correspondingly, the shape of the funnel-shaped area of the main body portion 1241 of the stirring assembly 124 provided in the groove portion 1221 can also be changed correspondingly. In detail, the main body portion 1241 of the stirring assembly 124 is arranged at the corresponding tapered position of the groove portion 1221, which can be designed as an airfoil and tapered toward the extension portion 1242, so that a good stirring effect can still be achieved when a small amount of reagent is used. .

Fig. 5 is an exploded view of the reagent storage unit of Fig. 1. Please refer to FIG. 3 and FIG. 5, the reagent containing unit 110 of this embodiment includes a containing structure 112. The reagent tanks 110a~110g are formed in the containing structure 112, and the bottom of the containing structure 112 has a plurality of channels 112a, and the channels 112a are connected to the reagent tanks 110a~110g respectively.道 Unit 130.

Furthermore, the reagent containing unit 110 further includes a plurality of elastic sealing members 114 and a bottom plate 116. The elastic seals 114 are disposed at the bottom of the containing structure 112 and correspond to the channels 112a, respectively. The bottom plate 116 is assembled to the bottom of the containing structure 112 in a screw-locked manner, and restricts the elastic seals 114 to the containing structure 112 and the flow channel. Between units 130. FIG. 6 is a partial enlarged view of the nucleic acid extraction device 100 of FIG. 3. Please refer to FIG. 6, each elastic sealing member 114 has a top surface 114 a and a bottom surface 114 b opposite to each other, and has a through hole 114 c. The through hole 114 c extends from the top surface 114 a to the bottom surface 114 b. Each elastic sealing element 114 is disposed in the bottom plate 116, and the top surface 114a and the bottom surface 114b of each elastic sealing element 114 contact the receiving structure 112 and the flow channel unit 130, respectively. The through hole 114c is connected to the corresponding channel 112a, so that the reagent tanks 110a to 110g can be connected to the flow channel unit 130 through the corresponding channel 112a and the corresponding through hole 114c. The material of each elastic sealing member 114 can be rubber or other suitable elastic materials, and can be sealed between the containing structure 112 and the flow channel unit 130 to avoid unexpected leakage of the reagent there. In addition, each channel 112a of the containing structure 112 is, for example, a capillary circuit, and the capillary resistance thereof can further prevent the leakage of the reagent.

In more detail, each elastic sealing member 114 is disposed in the opening 116a of the bottom plate 116, and each elastic sealing member 114 has a stepped shape as shown in FIG. 6, and can be restricted by the flange 116b in the opening 116a. At the bottom of the containment structure 112. The through hole 114c of each elastic sealing member 114 is adapted to communicate with the end 130a1 (marked in FIG. 6) of the flow channel 130a (marked in FIGS. 2 and 3) between the upper plate body 132 and the lower plate body 134 of the flow channel unit 130 ).

In this embodiment, the flow channel unit 130 has, for example, only a single flow channel 130 a, and one end of the flow channel 130 a is connected to the mixing tank 122. The reagent containing unit 110 is rotatably disposed on the flow channel unit 130 along the rotation axis RA (shown in FIG. 1) and is adapted to rotate so that any reagent tank 110a~110g corresponds to the other end of the flow channel 130a (i.e., FIG. 6 The shown end 130a1), so that one of the reagent tanks 110a to 110g is connected to the mixing tank 122. In this embodiment, for example, a motor or other suitable driver is used to drive the reagent containing unit 110 to rotate. In other embodiments, the communication between the reagent tanks 110a-110g and the mixing tank 122 can be switched by other appropriate methods and structures, and this creation is not limited to this.

Fig. 7 is a partial enlarged view of the accommodating structure of Fig. 1. Referring to FIG. 7, in this embodiment, the reagent tank 110d corresponding to the magnetic beads has an inlet 110d1, an outlet 110d2, and a guiding surface 110d3, and the bottom of the reagent tank 110d has a recess 110d4. The outlet 110d2 is located in the recess 110d4, and the recess 110d4 is suitable for accommodating magnetic beads. The position of the inlet 110d1 is higher than the outlet 110d2, and the guiding surface 110d3 extends obliquely from the inlet 110d1 to the outlet 110d2. When the magnetic beads are to be moved to the mixing tank 122 shown in FIG. 1, appropriate reagents can be driven into the reagent tank 110d from the inlet 110d1, so that the reagent drives the magnetic beads to move out of the reagent tank 110d from the outlet 110d2 and pass through the flow channel unit. 130 moves to the mixing tank 122. Since the magnetic beads are concentrated in the lower recess 110d4 in advance, and the reagent flows down from the inlet 110d1 above the position of the outlet 110d2 to drive the magnetic beads to move away from the reagent tank 110d through the outlet 110d2, so magnetism can be avoided. The beads are splashed out of the reagent tank 110d due to the impact of the reagent.

FIG. 8 shows the corresponding detection signal of the nucleic acid extracted by the nucleic acid extraction device of FIG. 1. As shown in FIG. 8, a significant detection signal appears between the nucleic acid length of 150-200 bp, indicating that the nucleic acid extraction device 100 of this embodiment can indeed extract nucleic acid from the specimen.

In summary, in addition to the existing tank, the mixing tank of the present invention also has a tube extending from the tank, and the stirring assembly has an extension extending into the tube correspondingly. In addition, there are first gaps and second gaps of different sizes between the extension portion of the stirring assembly and the inner wall of the pipe portion, that is, the size of the gap between the extension portion and the inner wall of the pipe portion is non-uniform. The uneven gap between the extension part of the stirring assembly and the pipe part makes the liquid produce uneven capillary force. When the pump sucks in from the upper end of the mixing tank, the liquid will flow up and down again and again in the pipe part, which can be attached to the pipe wall On the magnetic beads remelted.

100: Nucleic acid extraction device 110: Reagent containing unit 110a~110g: reagent tank 110d1: entrance 110d2: exit 110d3: guide surface 110d4: recess 112: containment structure 112a: Channel 114: Elastic seal 114a: top surface 114b: bottom surface 114c: Through hole 116: bottom plate 116a: opening 116b: flange 120: mixing unit 122: mixing tank 1221: Groove 1222: Pipe Department 1222a: Cylindrical pipe 124: Mixing component 1241: main body 1242: Extension 1243: connecting part 126: Actuator 128: cover 130: runner unit 130a: runner 130a1: end 132: Upper board body 134: Lower Board 140: heating device 150: Magnetic device 160: pump AD: axial G1: first gap G2: second gap L: first length RA: axis of rotation RD1: first direction RD2: second direction W: second length

Figure 1 is a perspective view of a nucleic acid extraction device according to an embodiment of the invention. Fig. 2 is an exploded view of the nucleic acid extraction device of Fig. 1. Fig. 3 is a cross-sectional view of the nucleic acid extraction device of Fig. 1. Fig. 4 is a cross-sectional view of the mixing unit of Fig. 1 along the line I-I. Fig. 5 is an exploded view of the reagent storage unit of Fig. 1. Fig. 7 is a partial enlarged view of the accommodating structure of Fig. 1. FIG. 8 shows the corresponding detection signal of the nucleic acid extracted by the nucleic acid extraction device of FIG. 1.

100: Nucleic acid extraction device

110: Reagent containing unit

110d: reagent tank

112: containment structure

112a: Channel

114: Elastic seal

114c: Through hole

116: bottom plate

120: mixing unit

122: mixing tank

1221: Groove

1222: Pipe Department

124: Mixing component

1241: main body

1242: Extension

1243: connecting part

126: Actuator

128: cover

130: runner unit

130a: runner

130a1: end

132: Upper board body

134: Lower Board

140: heating device

150: Magnetic device

160: pump

Claims (21)

A nucleic acid extraction device, including: A reagent containing unit adapted to contain at least one specimen, at least one magnetic bead and at least one extraction reagent; A mixing unit includes a mixing tank and a stirring assembly, wherein the mixing tank includes a tank portion and a pipe portion, the stirring assembly includes a main body portion and an extension portion, the main body portion is located in the tank portion, and the pipe portion is connected In the groove portion, the extension portion is connected to the main body portion and extends into the pipe portion, the extension portion and the inner wall of the pipe portion have a first gap in a first direction of the pipe portion, and the extension portion and The inner wall of the tube portion has a second gap in a second direction of the tube portion, and the first gap is smaller than the second gap; and The flow channel unit is connected between the reagent containing unit and the mixing unit, wherein the at least one specimen, the at least one magnetic bead, and the at least one extraction reagent are adapted to move from the reagent containing unit through the flow channel unit Into the mixing tank and stirred and mixed by the stirring component. The nucleic acid extraction device according to claim 1, wherein the inner width of the tube portion is smaller than the inner width of the groove portion. The nucleic acid extraction device according to claim 1, wherein the inner width of at least a part of the groove portion is tapered from top to bottom, and the main body portion is arranged in the corresponding tapered position of the groove portion to have an airfoil shape. The nucleic acid extraction device according to claim 1, wherein the width of the extension part is smaller than the width of the main body part. The nucleic acid extraction device according to claim 1, wherein the tube part is connected between the flow channel unit and the groove part. The nucleic acid extraction device according to claim 1, wherein the extension has a first length in the first direction and a second length in the second direction, and the first length is greater than the second length. The nucleic acid extraction device according to claim 1, wherein the tube portion has a cylindrical pipe, and the extension portion is located in the cylindrical pipe and has a rectangular cross section. The nucleic acid extraction device according to claim 1, wherein the tube portion has a rectangular pipe, and the extension portion is located in the rectangular pipe and has a circular cross section. The nucleic acid extraction device according to claim 1, wherein the mixing unit includes an actuator connected to the stirring assembly and adapted to drive the stirring assembly to rotate. The nucleic acid extraction device according to claim 1, further comprising a heating device, wherein the heating device is arranged beside the mixing tank and is suitable for heating the mixing tank. The nucleic acid extraction device according to claim 1, further comprising a magnetic attraction device, wherein the magnetic attraction device is movably arranged outside the tube, and is adapted to prevent the at least one magnetic bead from moving away from the tube by magnetic attraction Department. The nucleic acid extraction device according to claim 1, further comprising a pump, wherein the pump is connected to the mixing tank and is adapted to drive the at least one specimen, the at least one magnetic bead, and the at least one extraction reagent to move to the reagent Between the containing unit and the mixing tank. The nucleic acid extraction device according to claim 1, wherein the reagent accommodating unit has a plurality of reagent tanks, and the reagent tanks are adapted to respectively accommodate the at least one specimen, the at least one magnetic bead, and the at least one extraction reagent. The nucleic acid extraction device according to claim 13, wherein the at least one extraction reagent includes a lysis solution, a bonding solution, a washing solution, and an eluent, and some of the reagent tanks are suitable for accommodating the lysis solution and the The bonding liquid, the washing liquid, and the eluent. The nucleic acid extraction device according to claim 13, wherein the reagent tank corresponding to the at least one magnetic bead has an inlet and an outlet, and the location of the inlet is higher than the outlet. The nucleic acid extraction device according to claim 15, wherein the reagent tank corresponding to the at least one magnetic bead has a guiding surface, and the guiding surface extends obliquely from the inlet to the outlet. The nucleic acid extraction device according to claim 15, wherein the bottom of the reagent tank corresponding to the at least one magnetic bead has a recess, the outlet is located in the recess, and the recess is suitable for accommodating the at least one magnetic bead. The nucleic acid extraction device according to claim 13, wherein the reagent accommodating unit includes an accommodating structure, the reagent tanks are formed in the accommodating structure, the bottom of the accommodating structure has a plurality of channels, and the channels are respectively connected to the reagents Tanks, each of the reagent tanks communicates with the flow channel unit through a corresponding channel. The nucleic acid extraction device according to claim 18, wherein each channel is a capillary channel. The nucleic acid extraction device according to claim 18, wherein the reagent accommodating unit further includes a plurality of elastic sealing members and a bottom plate, the elastic sealing members are disposed at the bottom of the accommodating structure and respectively correspond to the channels, and the bottom plate is assembled At the bottom of the accommodating structure and confining each elastic sealing element between the accommodating structure and the flow channel unit, each elastic sealing element has a top surface and a bottom surface opposite to each other and has a through hole, and the through hole extends from the The top surface extends to the bottom surface, each of the elastic seals is arranged in the bottom plate, and the top surface and the bottom surface respectively contact the containing structure and the flow channel unit, and the through hole is connected to the corresponding channel. The nucleic acid extraction device according to claim 13, wherein the flow channel unit has a flow channel, one end of the flow channel is connected to the mixing tank, and the reagent containing unit is rotatably disposed on the flow channel unit and is suitable for rotation. Make any one of the reagent tanks correspond to the other end of the flow channel.

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