TWI611806B - A use of an extract of asplenium australasicum (j. sm.) hook. - Google Patents

Abstract

The utility model relates to a water extract of Nanyang Mountain Soda extract, which is used for preparing a medicine for treating hepatitis C. The water extract of Nanyang Mountain Sophora is used as an extraction solvent at a temperature of 115 to 125 ° C and a temperature of 0.35 to 0.5. It was obtained by extracting a sample of Nanyangshansu under the pressure of Kg / cm ² G for 1.5 ~ 3 hours.

Description

Uses of Soyang Extract from Nanyang Mountain

The present invention relates to the use of a water extract from Nanyang Mountain Perilla, in particular to the use of a water extract from Nanyang Mountain Perilla to prepare a medicine for treating hepatitis C.

Hepatitis C is an infectious disease caused by the Hepatitis C virus. It has no symptoms in the early stages of infection, and occasionally has fever and yellow urine. symptoms such as dark urine, abdominal pain, or yellow tinged skin, and evolved to liver disease or even cirrhosis, or other complications, such as liver failure, over many years (liver failure), liver cancer, esophageal varices, or esophageal and gastric varices.

The combination therapy of interferon (peginterferon) and ribavirin often has a cure rate of about 50% and has strong side effects. Therefore, it is still necessary to develop new anti-hepatitis C drug.

In order to solve the above problems, the present invention provides the use of a water extract from Nanyang Mountain Perilla, which is an active ingredient extracted from Nanyang Mountain Perilla, and is used to prepare a medicine for treating hepatitis C.

The use of the Nanyang Mountain Soda extract is used for preparing medicine for treating hepatitis C, wherein the Nanyang Mountain Souce extract is water at a temperature of 115-125 ° C and 0.35 ~ 0.5Kg / cm ² It is obtained by extracting for 1.5 ~ 3 hours under the pressure of G. In this way, by extracting the active ingredients from Nanyangshansu, it can inhibit the replication of hepatitis C virus, so it can be applied to the preparation of drugs for the treatment of hepatitis C. efficacy.

FIG. 1 is a line chart showing the results of the effect of administering the extract of Nanyang Mountain Solanum nigra on the survival rate of human liver cancer cell lines containing HCV replicons.

Fig. 2a is an image showing the results of the effect of administering the extract of Nanyang Mountain Solanum nigrum L. to the protein content of non-structural protein NS5B of a human liver cancer cell line containing HCV replicon.

Fig. 2b: Histogram of the results of the effect of administering the extract of Nanyang Mountain Solanum officinalis on the RNA content of nonstructural protein NS5B of a human liver cancer cell line containing HCV replicon.

FIG. 3 is an image showing the result of the effect of administering the extract of Nanyang Mountain Solanum nigrum L. to the protein content of non-structural protein NS5B of a human liver cancer cell line containing HCV replicon.

Figure 4: Histogram of the effect of the administration of the extract of Nanyang Mountain Solanum nigrum L. on the RNA content of the non-structural protein NS5B of a human liver cancer cell line infected with hepatitis C.

In order to make the above and other objects, features, and advantages of the present invention more comprehensible, the following describes the preferred embodiments of the present invention and the accompanying drawings in detail, as follows: "Su ( Asplenium australasicum (J.Sm.) Hook.)" Refers to a plant of the genus Asplenium of the Aspleniaceae family, and its young emerging frond is often used as a vegetable leaf.

The water extract of Nanyang Mountain Sophora according to the present invention can inhibit the replication of Hepatitis C virus, so it can be applied to the preparation of drugs for the treatment of Hepatitis C. The excipients are combined to form a pharmaceutical composition, wherein the Nanyangshansu water extract can be prepared into any convenient form, such as tablets, capsules, powders, granules or liquids, etc. Water extracts are combined with other foods or beverages to make them suitable for consumption This state is for the organism to take orally.

Among them, the Nanyangshansu water extract is preferably prepared by a method including the following steps: providing a Nanyangshansu sample; using water as an extraction solvent to extract the Nanyangshansu sample; and The crude extract was concentrated to obtain the Nanyangshansu extract.

In detail, the Nanyangshansu sample can be the mature old leaves of Nanyangshansu (more than three years, with spores distributed on the back). Preferably, the Nanyangshansu sample can be processed in advance at a temperature of 110 ~ 120 ° C. Drying (drying 100g Nanyangshansu sample to 20g) to obtain a dried Nanyangshansu sample; In addition, the Nanyangshansu sample can also be crushed into powder particles (particle size about 1 ~ 2mm) in advance to increase the The contact surface area of the Nanyang Shansu sample and the extraction solvent improves the extraction efficiency of subsequent extraction.

For example, every 4.8kg of Nanyangshansu sample can be mixed with the extraction solvent so that the total volume of the two reaches 80L, and the extraction is performed at a temperature of 115 to 125 ° C and a pressure of 0.35 to 0.5 Kg / cm ² G. ~ 3 hours, the above extraction can also be repeated several times, so that the active ingredients contained in the Nanyangshansu sample can be completely dissolved in the extraction solvent. This is widely used by those with ordinary knowledge in the technical field to which the present invention belongs. Do not repeat them.

The aforementioned crude extract of Nanyang Mountain Perilla can be concentrated under reduced pressure and freeze-dried to obtain the Nanyang Mountain Perilla water extract. With this procedure, the active ingredients of the Nanyang Perilla water extract can be more concentrated. Therefore, only a small amount of the Nanyangshan sour water extract can be used to achieve the best effect.

In order to confirm that the water extract of Nanyang Mountain Sorbaria according to the present invention can inhibit the replication of hepatitis C virus, the following tests were performed.

(A) Cytotoxicity of water extract from Nanyang Mountain Sophora to liver cancer cells

Please refer to Table 1. This test was performed using the human liver cancer cell line Ava5 containing HCV replicons. The test was performed at 0, 10, 25, 50, 75, 100, 150, 200, 300, and 400 μL / mL Nanyang. The hepatocellular carcinoma cells were treated with _Sansu water extract, and the cell survival rate of the hepatocellular carcinoma cells was observed with a cytotoxicity test kit (CellTiter 96 AQ _ueous One Solution Proliferation Assay) three days later. The cell viability of the cells was taken as 100%.

Please refer to Figure 1. Nanyang Mountain Soy extract has no obvious toxicity at a concentration of 50 μL / mL or less.

(B) Effect of extracts from Nanyang Mountain Soda on replication of hepatitis C virus

As shown in Table 2, this test was also performed on the human liver cancer cell line Ava5 containing HCV replicon, and the liver cancer cells that were not treated with the Nanyangshan thaliana water extract were used as the positive control group (Group B0). The liver cancer cells were treated with a water extract of Nanyang Mountain Solanum at a concentration of 10 μL / mL (Group B1), 25 μL / mL (Group B2), 50 μL / mL (Group B3), and 75 μL / mL (Group B4) for three days. Then, after lysing the cells of each group, the protein content of non-structural protein NS5B in each group of cells was analyzed by Western blot method.

Please refer to Figure 2a, the protein content of the non-structural proteins NS5B in groups B1 to B3 has been reduced, indicating that the Nanyang Mountain Sophora extract can inhibit the replication of hepatitis C virus.

Continue to use real-time polymerase chain reaction to detect the RNA content of non-structural protein NS5B in cells of groups B0 ~ B4. As shown in Figure 2b, the RNA content of non-structural protein NS5B of groups B1 to B4 also decreased. It has been shown that the Nanyangshansu water extract can inhibit the replication of hepatitis C virus.

(C) The effect of long-term treatment of the extracts from Nanyang Mountain

As shown in Table 3, this test was performed according to the above procedure, but the protein content of the non-structural protein NS5B in each group of cells was analyzed after changing the liver cancer cells for six days with different concentrations of Nanyangshansu water extracts. .

Please refer to Figure 3, the protein of group C2 non-structural protein NS5B The content was significantly reduced, showing that the Nanyang Mountain Solanum extract can inhibit the replication of hepatitis C virus.

(D) Effect of extracts from Nanyang Mountain Soda on replication of hepatitis C virus

Please refer to Table 4. This test was performed using human liver cancer cell line Huh-7. After Hepatitis C virus infection (MOI = 0.1), the test was performed at 0, 10, 25, and 50 μL / mL. The liver cancer cells were treated with the extract, and the RNA content of the non-structural protein NS5B in the cells of groups D0 to D4 was detected by real-time polymerase chain reaction after three days.

Please refer to Figure 4, the RNA content of the non-structural protein NS5B of groups D2 to D4 also decreased, indicating that the Nanyang Mountain Solan extract can inhibit the replication of hepatitis C virus.

Based on the results of the above tests, the extract of Nanyangshan sour water can effectively inhibit the replication of the hepatitis C virus strain, and thus can eliminate the hepatitis C virus in the body to achieve the effect of treating hepatitis C.

In addition, a patient suffering from benign prostatic hypertrophy who also had a 15-year history of hepatitis C had a liver index GPT of 3 months after taking the Nanyangshan Sushui extract of the present invention. Successfully reduced to below 20KU / mL; 10 other patients with hepatitis C (including male and female) took the Nanyangshan Sushui extract of the present invention for 4 months, and the liver index GPT had been successfully reduced to normal value.

In summary, the use of the water extract of Nanyang Mountain Perilla in the present invention is to extract the active ingredients of Nanyang Mountain Perilla, which can inhibit the replication of Hepatitis C virus and clear the hepatitis C virus in the body, so it can be applied to the preparation of Type C The medicine for treating hepatitis is the effect of the present invention.

Although the present invention has been disclosed using the above-mentioned preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various changes and modifications to the above embodiments without departing from the spirit and scope of the present invention. The technical scope protected by the invention, so the scope of protection of the present invention shall be determined by the scope of the appended patent application.

Claims (1)

The utility model relates to a water extract of Nanyang Mountain Perilla, which is used for preparing a medicine for treating hepatitis C. The water extract of Nanyang Mountain Perilla uses water as an extraction solvent at a temperature of 115-125 ° C and 0.35 ~ 0.5Kg. It was obtained by extracting a sample of Nanyangshansu under a pressure of 1.5 g / cm ² G for 1.5 ~ 3 hours.