TWI586333B - A blood volume detecting method and a detecting device using the same - Google Patents

Description

Blood specific volume detecting method and detecting device using the same

The present invention relates to a detection method, and more particularly to a method for detecting hematocrit (Hct) in blood and a detection device using the same.

Hematocrit (Hct), also known as the percentage of blood volume, refers to the proportion of red blood cells contained in a certain amount of blood, and is often used as an indicator for diagnosing anemia or cardiovascular disease. However, for the measurement of blood glucose level, hematocrit is often one of the key factors affecting the blood glucose measurement. Therefore, in order to accurately measure the blood glucose level, it is often necessary to measure the blood specific volume of the subject first, and then according to The hematocrit is corrected for blood glucose measurements.

Traditional blood specific capacity tests mostly use manual or special machines (such as blood cell counting machines). The former has the disadvantages of complicated operation and time consuming, while the latter requires high cost disadvantages of procurement and maintenance.

Therefore, is there a way or mechanism to increase the blood specific capacity measurement? Accuracy and reliability have become the subject of the present invention.

According to one aspect of the present invention, there is provided a method of detecting hematocrit (Hct) in blood and a detecting apparatus using the same.

The blood specific volume detecting method of the present invention comprises: providing a pair of detecting electrodes, applying a blood to a pair of detecting electrodes, and applying a voltage value to the pair of detecting electrodes; and turning off the applied voltage value to obtain a discharging current And obtaining a precise hematocrit of the blood according to a predetermined interpretation rule and the discharge current value.

The detecting device using the method of the present invention comprises: a detecting unit including a pair of detecting electrodes having a receiving portion and a contact portion, wherein the receiving portion is configured to receive a blood; and a detector connected to the contact portion of the pair of detecting electrodes, and according to a preset determining rule The contact portion applies a voltage value and then turns off the applied voltage value to obtain a hematocrit of the blood.

The method for measuring the blood specific capacity of the discharge current when the electrode test piece is discharged by the present invention can greatly improve the accuracy and reliability.

10‧‧‧Detection unit

100‧‧‧Substrate

110‧‧‧Detection electrode

111‧‧‧ Receiving Department

112‧‧‧Contacts

130‧‧‧Baffle

131‧‧‧ openings

140‧‧‧ cover

141‧‧ ‧ guide

142‧‧‧ Guide hole

20‧‧‧Detector

210‧‧‧Connector

220‧‧‧reference voltage source

230‧‧‧ Controller

FIG. 1 is a flow chart of a blood specific volume detecting method of the present invention.

Figure 2 is a schematic diagram of voltage values applied to the present invention.

Figure 3 is a schematic diagram showing the shutdown voltage after applying a voltage value according to the present invention.

Fig. 4 is a graph showing experimental data of current time of five different blood glucose concentrations when the hematocrit of the present invention is Hct 61%.

Fig. 5 is a graph showing the current time when the blood glucose concentration of the present invention is 25 mg/dl.

Fig. 6 is a current time chart of the blood glucose concentration of the present invention at 166 mg/dl.

Fig. 7 is a graph showing the current time when the blood glucose concentration of the present invention is 311 mg/dl.

Figure 8 is a graph showing the current time at a blood glucose concentration of 450 mg/dl.

Fig. 9 is a graph showing the current time when the blood glucose concentration of the present invention is 595 mg/dl.

Figure 10 is a current time combination diagram of Figures 5 through 9.

Fig. 11 is a graph showing the experimental data of the current time in the case where the hematocrit is Hct 0%, Hct 21%, Hct 61%, and Hct 72%.

Figure 12 is a graph showing the current time when the hematocrit is plotted as Hct 0% according to the data in Figure 11.

Figure 13 is a graph showing the current time when the hematocrit is plotted as Hct 21% according to the data in Figure 11.

Figure 14 is a graph showing the current time when the hematocrit is plotted as Hct 61% according to the data in Figure 11.

Figure 15 is a graph showing the current time when the hematocrit is plotted as Hct 72% according to the data in Figure 11.

Figure 16 is a schematic view showing an embodiment of a hematocrit detecting device of the present invention.

Figure 17 is an exploded perspective view showing an embodiment of a detecting unit in the hematocrit detecting device of the present invention.

Figure 18 is a schematic view showing an embodiment of a detecting unit in the hematocrit detecting device of the present invention.

The following describes the blood specific volume detecting method of the present invention. Please refer to FIG. 1 , which is a flowchart of the blood specific volume detecting method of the present invention. The blood specific volume detecting method step includes: Step 1: providing a pair of detecting electrodes, And applying a blood to the pair of detecting electrodes, and then applying a voltage value to the pair of detecting electrodes (S20); Step 2: turning off the applied voltage value to measure a discharge current value (S22); and 3: Obtaining a blood specific volume of the blood according to a preset interpretation rule and the discharge current value (S22).

Step 3: Obtain a blood specific volume of the blood according to a preset interpretation rule and the discharge current value (S22).

Wherein, the voltage is applied to the pair of detecting electrodes in step 1, the voltage value may be between 1 and 3 volts, and the voltage may be applied between 0.01 and 1 second.

In the first step, the closer the distance between the pair of detecting electrodes is, the larger the capacitance is. In a preferred embodiment, the distance between the two detecting electrodes is 0.6 mm to 0.05 mm.

Wherein, in step 2, the applied voltage value is turned off to measure a discharge current value, because the discharge current at the time of discharge is large, and the resolution test as the judgment is better, and the measurement effect is better, so that The measured discharge current value was good within 0.005 seconds after discharge.

Wherein, the preset interpretation rule of step 3 includes a plurality of hematocrit data, wherein the data refers to a relationship between a hematocrit and a discharge current value at different voltage values, thereby determining the blood of the blood to be measured. specific volume.

The reason why the blood specific volume detecting method of the present invention is more accurate is as follows. Please refer to FIGS. 2 and 3 for explaining that the voltage is not turned off and a response current value is obtained by the voltage value, and the voltage is turned off and the discharging current state is Obtaining a comparison of the two states of the discharge current value; as shown in FIG. 2, after applying a voltage value to the pair of detecting electrodes 110, the pair of detecting electrodes 110 are in a state of capacitive charging, and a response current is generated, the current of the response current The direction and state are shown in Figure A. At this time, the response current is not only affected by the redox reaction, but also affected by the reaction of blood glucose concentration, cholesterol or urea, resulting in the response under the same hematocrit (Hct). The current is significantly different, and it is not accurate enough to obtain a hematocrit of the blood by the response rule and the interpretation rule of the relationship between the hematocrit and the response current value according to different voltage values.

As shown in FIG. 3, when the pair of detecting electrodes 110 is applied with a voltage value for a period of time, and then the voltage is turned off, the pair of detecting electrodes 110 is in a capacitor discharging state, and the current direction and state thereof are as shown in FIG. The discharge state will be generated, and the discharge current is not affected by the redox reaction, and is only affected by the hematocrit (Hct). Therefore, in step 3 of the present invention, one of the blood is obtained according to a preset interpretation rule and the discharge current value. Blood specific volume, the blood volume obtained is more accurate.

The blood specific volume detection method of the present invention is not affected by blood glucose concentration by experimental data. Please refer to FIG. 4 to FIG. 10 , wherein the fifth to the ninth figures are blood glucose drawn according to the data of FIG. 4 . Current time plots at concentrations of 25 mg/dl, 166 mg/dl, 311 mg/dl, 450 mg/dl, and 595 mg/dl, and Figure 10 is a plot of current time in Figures 5 through 9, and Figure 4 is blood. The current time experimental data of five different blood glucose concentrations when the specific volume is Hct 61%, where the unit of current is amperes (A), and the unit of time is seconds (SEC), where the current of the graphs drops sharply, This is the point in time at which the applied voltage value is turned off.

As shown in Fig. 5 to Fig. 9, when a voltage value is applied and then the voltage is turned off, it is region C when the current is discharged, and the measurement in the region C is not affected by blood glucose concentration, cholesterol or urea. Therefore, the hematocrit of the discharge current generated during this period will be more refined. Indeed. As shown in Fig. 10, when the same hematocrit is Hct 61%, the discharge currents generated by the five different blood glucose concentration samples in the discharge region C are almost the same, and the current generated during this period is taken. The hematocrit will be more accurate, but during the application of the voltage value to the pair of detection electrodes and the application of the voltage value has not been turned off, the respective response currents of the five different blood glucose concentration samples are significantly different, and thus are generated during this period. The hematocrit of the response current will be less accurate.

The blood specific volume detecting method of the present invention will be further described below with experimental data. Please refer to FIG. 11 to FIG. 15 , and FIG. 11 is a current with a hematocrit of Hct 0%, Hct 21%, Hct 61%, and Hct 72%. Time experimental data plot, where the current is in amps (A) and the time is in seconds (SEC). The 12th to 15th drawings are current time charts of blood specific volume of Hct 0%, Hct 21%, Hct 61%, and Hct 72%, respectively, according to the data in FIG. 11 . The point in time at which the current drops sharply is the point in time at which the applied voltage value is turned off.

Please refer to Fig. 11 and Fig. 12 at the same time, showing that the applied voltage is turned off at 0.25 seconds when the hematocrit is Hct 0%, and the discharge current is measured to be -1.44E-04 ampere at 0.255 seconds; Referring to Fig. 11 and Fig. 13 at the same time, it shows that when the hematocrit is Hct 21%, the applied voltage is turned off at 0.25 seconds, and the discharge current is measured to be -1.38E-04 ampere at 0.255 seconds; Referring again to Figures 11 and 14, it is shown that when the hematocrit is Hct 61%, the applied voltage is turned off at 0.25 seconds, and the discharge current is measured to be -1.23E-04 ampere at 0.255 seconds; Referring again to Fig. 11 and Fig. 15, it shows that the applied voltage is turned off at 0.25 seconds when the hematocrit is Hct 72%, and the discharge current is measured to be -1.14E-04 ampere at 0.255 seconds; It can be seen from the above that the discharge current is significantly different at different hematocrit, and therefore, according to the method of the present invention, the hemorrhagic volume can be accurately determined.

Hereinafter, a detecting device for detecting a hematocrit method using the present invention will be described by way of examples. Please refer to FIG. 16, which is a schematic diagram of an embodiment of a hematocrit detecting device of the present invention. The hematocrit detecting device includes a detecting unit 10 and a detector 20. The detecting unit 10 includes a pair of detecting electrodes 110 having a receiving portion 111 and a contact portion 112 for receiving a blood. The detector 20 is connected to the contact portion 112 of the pair of detecting electrodes 110, and applies a voltage value to the contact portion 112, and then turns off the applied voltage value, and according to a predetermined judgment Read the rules to get the blood specific volume of the blood.

The detector 20 includes a pair of ports 210, a reference voltage source 220, and a controller 230. A first end of each of the ports 210 is connected to the contact portion 112. The reference voltage source 220 is connected to one of the second ends of the port 210 for providing the voltage value required for detection. The controller 230 is connected to the second terminal of the other connection port 210 and the reference voltage source 220. The reference voltage source 220 is pressurized for a period of time and the application voltage is turned off, and is received by the detection unit 10 The discharge current value is used to obtain the hematocrit of the blood.

As shown in Fig. 17, Fig. 17 is an exploded perspective view showing an embodiment of a detecting unit in the blood specific volume detecting device of the present invention, and Fig. 18 is a view showing an embodiment of a detecting unit in the blood specific volume detecting device of the present invention. In an embodiment, as shown in FIG. 17, the detecting unit 10 can be a test piece design, and includes a substrate 100, the pair of detecting electrodes 110, a partition plate 130, and a cover plate 140. The pair of detecting electrodes 110 are disposed on the substrate 100. The spacer 130 is disposed on the substrate 100 and partially covers the pair of detecting electrodes 110 such that the end electrode portions of the pair of detecting electrodes 110 are exposed and included in an opening 131. The opening 131 is formed at a front end of the partition plate 130 such that the pair of detecting electrodes 110 are exposed corresponding to the front end electrode portion of the opening 131.

Here, the receiving portion 111 of the pair of detecting electrodes 110 is defined as a region exposed at the position of the opening 131, and the contact portion 112 is defined as a rear end electrode of the pair of detecting electrodes 110 not covered by the spacer 130.

The cover plate 140 is disposed on the partition plate 130 and includes a guide port 141 and a guide hole 142. The guide port 141 is disposed at a front end of the cover plate 140 and overlaps with the opening 131 of the partition plate 130. The guide hole 142 is defined in the position of the cover plate 140 corresponding to the opening 131 of the partition plate 130, and the opening 131 A conduction path is formed.

When a user takes blood through a special lancet, blood is dripped in the guide hole 142, and the conduction path formed by the guide hole 142 and the opening 131 of the spacer 130 generates a capillary phenomenon for allowing blood to pass through. The opening 131 is introduced into the detecting unit 10 and is in contact with the pair of detecting electrodes 110. Then, the contact portion 112 of the pair of detecting electrodes 110 is connected to the pair of ports 210 of the detector 20, and then the detector 20 is activated.

The controller 230 drives the reference voltage source 220 to apply a voltage value between the pair of detecting electrodes 110, the applied voltage is dependent on the blood to generate an electrochemical reaction, thereby forming a conduction path having a response current; After the applied voltage value is turned off, a discharge current is generated, and the discharge current value changes due to the hematocrit of the blood; the controller 230 reads the discharge current value to determine the hematocrit of the blood to be measured. The controller 230 can distinguish the hematocrit of the blood for different discharge current values in one unit time.

In order for the controller 230 to perform the hematocrit interpretation on the received discharge current value efficiently and accurately, the controller 230 needs to be set in advance (for example, through an electrochemical device) to set a blood ratio of different percentage values. The discharge current data of the capacitor is pre-stored in the controller 230 for conversion of the detected discharge current each time.

The reason is that compared with the detection of the traditional hematocrit, the blood specific capacity measurement of the discharge current when the electrode test piece is discharged in the present invention can greatly improve the accuracy and reliability.

However, the above-mentioned preferred embodiments of the present invention are not intended to limit the scope of the invention, and any changes and modifications apparent to those skilled in the art should be considered as not The substance of the invention is removed.

Claims (10)

A blood specific volume detecting method comprising: Step 1: providing a pair of detecting electrodes, applying a blood to the pair of detecting electrodes, and then applying a voltage value to the pair of detecting electrodes; Step 2: turning off the applied voltage value And obtaining a discharge current value by measuring; and step 3: obtaining a blood specific volume of the blood according to a preset interpretation rule and the discharge current value. The hematocrit detecting method according to claim 1, wherein in step 1, a voltage value is applied to the pair of detecting electrodes, and the voltage value is between 1 and 3 volts. The hematocrit detecting method according to claim 1, wherein the step 1 applies a voltage value to the pair of detecting electrodes, and the voltage is applied for a time between 0.01 and 1 second. The blood specific volume detecting method according to claim 1, wherein the distance between the pair of detecting electrodes in step 1 is 0.6 mm to 0.05 mm. The blood specific volume detecting method according to claim 1, wherein the voltage value applied in step 2 is turned off to measure a discharge current value, which is 0.005 seconds after the applied voltage value is turned off. The discharge current value is measured internally. The blood specific volume detecting method according to claim 1, wherein the preset interpretation rule of the step 3 includes a plurality of hematocrit data, wherein the data refers to a hematocrit and a discharge current at different voltage values. The relationship between the amount of change in value and the blood specific volume of the blood to be measured is determined accordingly. A blood specific volume detecting device used in the blood specific volume detecting method of the first application of the patent application, comprising: a detecting unit comprising a pair of detecting electrodes having a receiving portion and a contact portion, wherein the receiving portion is used for Receiving a blood; and a detector connecting the contact portion of the pair of detecting electrodes, applying a voltage value to the contact portion according to a preset determination rule, and then turning off the voltage value applied to the contact portion to obtain the One of the blood is hematocrit. The hematocrit detecting device of claim 7, wherein the detecting device further comprises: a pair of connecting ports, wherein the first end of each of the connecting ports is connected to the contact portion; a reference voltage source connected to one of the second ends of the port for providing the voltage value; and a controller connected to the second end of the other port and the reference voltage source, And obtaining the blood specific volume of the blood by receiving a discharge current value. The blood specific volume detecting device according to claim 7, wherein the detecting unit further comprises: a substrate for the pair of detecting electrodes disposed thereon; and a partition plate disposed on the substrate, the portion thereof Covering the pair of detecting electrodes, the end electrode portions of the pair of detecting electrodes are exposed, and an opening is formed at a front end of the partition, so that the pair of detecting electrodes are exposed corresponding to the front end electrode portion of the opening; and a cover plate Provided on the partition plate, and comprising: a guide port disposed at a front end of the cover plate and overlapping with the opening of the partition plate; and a guide hole opened at an opening position of the cover plate corresponding to the partition plate, Forming a conduction path with the opening. The hematocrit detecting device according to claim 8, wherein the controller pre-sets to store pre-storage current data of different specific values of hematocrit in the controller.