TWI499774B - Biosensors and bio-measurement systems - Google Patents

Description

Biosensor and biometric system

The present invention relates to a biosensor, and more particularly to a biosensor having a code.

The current blood glucose meter mostly uses the fingertip blood to chemically react with the enzyme on the biochemical test strip to achieve blood glucose measurement. Due to manufacturing process limitations, biochemical test strips manufactured in different batch processes have different characteristics, such as enzyme reaction characteristics, which will affect the measurement of blood glucose. Therefore, in the manufacturing process of each batch of biochemical test strips, the manufacturer needs to set a code for the enzyme reaction characteristics of the batch of biochemical test strips. When the user uses the blood glucose meter, the code can be entered through the keyboard on the blood glucose meter, or the code card with the code programmed can be inserted into the blood glucose meter for reading. When the blood glucose meter obtains these codes, the parameters of the blood glucose measurement operation can be changed or set so that the blood glucose measurement results are not affected by changes in the enzyme reaction characteristics.

However, in practice, the user may enter the wrong code, or the user may forget to insert the password card or insert the wrong password card. These conditions will lead to deviations in blood glucose measurement, which may delay the timing of the user's treatment or take the deviation of the drug dose, which is harmful to the user's life.

Accordingly, it is desirable to provide a biosensor for sensing a biological sample having a code representative of the characteristics of the biosensor. When the biosensor is connected to the biometric device, the biometric device can automatically read the biosensor code, thereby reducing the risk of misjudging the measurement result and improving the operational convenience of the overall biometric system.

According to an embodiment of the invention, the invention provides a biosensor for sensing a biological sample. The biosensor has a first code representing a characteristic of the biosensor and a second code. The biosensor includes a substrate and a conductive layer. The conductive layer is disposed on the first side of the substrate and includes a first loop, a second loop, and a third loop. The first loop is formed between the first end point and the second end point and has a first impedance. The second loop is formed between the third end point and the fourth end point and has a second impedance. The second end point and the third end point are electrically insulated from each other. The third loop is formed between the fourth end point and the fifth end point and has a third impedance. The first impedance and the second or third impedance define a first code, and the first impedance and the second or third impedance define a second code.

In accordance with another embodiment of the present invention, the present invention provides a biometric system for sensing an analyte of a biological sample. The biometric system includes a biosensor and a biometric device. The biosensor has a first code representing the characteristics of the biosensor and a second code, and includes a substrate, a bioreactive layer, and a conductive layer. The substrate has a first side and a second side opposite the first side. The bioreactive layer is disposed in the biological reaction zone of the first side and has a chemical reagent. Biological samples can be placed in the bioreactor zone. The conductive layer is disposed on the second side and includes a first loop, a second loop, and a third loop. The first loop is formed between the first end point and the second end point and has a first impedance. The second loop is formed between the third end point and the fourth end point and has a second impedance. The first loop and the second loop are electrically insulated from each other. The third loop is formed between the fourth end point and the fifth end point and has a third impedance. The biosensor can be connected to a biometric device. The biometric device measures the first impedance, the second impedance, and the third impedance, and calculates a first code according to the first impedance and the second or third impedance and calculates according to the first impedance and the second or third impedance The second code. The biometric device performs a measurement operation on the analyte of the biological sample according to the first code and the second code.

The above described objects, features and advantages of the present invention will become more apparent from the description of the appended claims.

Fig. 1 is a side view showing a biosensor according to an embodiment of the present invention. The biosensor 1 is used to sense an analyte of a biological sample. Referring to FIG. 1, the biosensor 1 has a substrate 10, a conductive layer 11, and a biological reaction layer 12. The conductive layer 11 is disposed on one side 10a of the substrate 10, and the bioreactive layer 12 is disposed on the other side 10b opposite to the side 10a of the substrate 10.

Fig. 2 shows the side surface 10b of the aforementioned substrate 10. Referring to Figure 2, the bioreactive layer 12 is disposed within the bioreactor 20 of the side 10b. The biological reaction layer 12 has a chemical reagent. When a biological sample taken from a user is dropped, inhaled, or placed in the biological reaction zone 20, at least one analyte in the biological sample chemically reacts with the chemical reagent. For example, the biological sample can be the blood of the user, the analyte is glucose (blood sugar) in the blood, and the chemical reagent of the biological reaction layer 12 is an enzyme. When the user's blood is dripped, inhaled or placed in the biological reaction zone 20, the glucose in the blood chemically reacts with the chemical reagent (enzyme). It should be noted that in the first and second figures, the size of the biological reaction layer 12 and the biological reaction zone 20 is only an exemplary example. In practical applications, the size of the biological reaction layer 12 and the biological reaction zone 20 can be determined according to factors such as the size of the biosensor 1, the amount of biological sample sampling, and actual demand.

Figure 3A shows the side 10a of the substrate 10. Referring to FIG. 3A, five terminals A, B, C, D, and E are disposed at the lower edge of the conductive layer 11. The conductive layer 11 has a code pattern defining two codes Code1 and Code2 representing the characteristics of the biosensor 1. In this embodiment, the two codes Code1 and Code2 represent manufacturing information of the biosensor 1, such as the reaction characteristics of the chemical reagent of the biological reaction layer 12, the manufacturing time (date or number of weeks) of the biosensor 1, or Correction information of the biosensor 1 and the like. This code pattern includes a loop formed between endpoints A and B, a loop between endpoints C and D, and a loop between endpoints D and E. For clarity, the code pattern of the conductive layer 11 is shown in Figure 3B. Referring to Figure 3B, loop 30 is formed between endpoints A and B, loop 31 is formed between endpoints C and D, and loop 32 is formed between endpoints D and E. The terminals B and C are electrically insulated from each other. In other words, the loop 31 and the loop 32 are electrically insulated from the loop 30, respectively. In the first and third embodiments, the size of the conductive layer 11 is only an exemplary example. In practical applications, as described above, one of the design basis of the size of the conductive layer 11 is the size of the code pattern.

In the embodiment of the invention, the impedance of loop 30 and the impedance of loop 31 define code Code1, while the impedance of loop 30 and the impedance of loop 32 define code Code2. The respective impedances of loops 30, 31, and 32 can be varied by varying the respective line widths or lengths. For example, where the loops 30, 31, and 32 have substantially the same linewidth, the respective lengths of the loops 30, 31, and 32 determine their respective impedances.

In one embodiment, loops 30, 31, and 32 are disposed on substrate 10 in a screen printing technique. When circuits 30, 31, and 32 are printed with different slurries, loops 30, 31, and 32 may have different impedances even under substantially the same line width and length conditions.

Figure 4 is a diagram showing a biometric system in accordance with an embodiment of the present invention. Referring to FIG. 4, the biometric system 4 includes the biosensor 1 of FIG. 1 and a biometric device 40. In one embodiment, the biometric system 4 is a blood glucose measurement system. The blood glucose measurement system will be described below as an example. When the user wants to measure the glucose concentration in the blood, the biosensor 1 is first inserted into the slot 41 of the biometric device 40, and then the blood is dropped, inhaled or placed in the biological reaction zone 20. Referring to Figures 2 and 4, the sides 10b of the substrate 10 are further provided with electrodes 21 and 22 which are connected to the bioreactive layer 12. When the glucose in the blood is electrochemically reacted with a chemical reagent (enzyme), the biometric device 40 can pass through the electrodes 21 and 22 to obtain an electrical signal caused by the above electrochemical reaction to measure the glucose concentration in the blood. operating.

Further, when the biosensor 1 is inserted into the slot 41 of the biometric device 40, the biometric device 40 measures the respective impedances of the loops 30, 31, and 32. After the biometric device 40 knows the respective impedances of the loops 30, 31, and 32, the biometric device 40 calculates the code Code1 of the biosensor 1 based on the impedance of the loop 30 and the impedance of the loop 31, and according to the loop 30. The impedance of the circuit and the impedance of the loop 32 are used to calculate the code Code2 of the biosensor 1. Next, the biometric device 40 sets at least one parameter of the measurement operation according to the codes Code1 and Code2, so that the biometric device 40 can perform the measurement operation on the glucose concentration of the blood according to the parameter. By measuring the glucose concentration, for example, at least one parameter set according to the two codes Code1 and Code2 of the biosensor 1 is related to the reaction characteristics of the enzyme as a chemical reagent in the biological reaction layer 12. In this way, even if the biosensor 1 manufactured by a different batch process is used, the biometric device 40 can accurately measure the glucose concentration in the blood of the user. The measurement results are not affected by different reaction characteristics caused by different batch processes, and the measurement accuracy is improved.

After the biometric device 40 measures the glucose concentration in the blood, the measurement result can be displayed on one of the display panels 42 of the biometric device 40 for reading by the user or medical personnel.

How the biometric device 40 calculates the codes Code1 and Code2 of the biosensor 1 through the respective impedances of the measurement circuits 30, 31, and 32 will be described below.

Referring to FIG. 5A, when the biosensor 1 is inserted into the slot 41 of the biometric device 40, the biometric device 40 couples one end of the loop 30, such as the end point B, to the ground potential GND. At the same time, the biometric device 40 provides a specific voltage V50 to the endpoint A. At this time, the current I50 flowing from the end point A to the end point B is caused on the loop 30 in accordance with the impedance of the loop 30 and the specific voltage V50 described above. Biometric device 40 measures current I50 at endpoint A.

Referring to FIG. 5B, when the biosensor 1 is inserted into the slot 41 of the biometric device 40, the biometric device 40 couples the terminal D to the ground potential GND. At this time, the biometric device 40 provides a specific voltage V50 to the end point C. At this time, the current I51 flowing from the terminal C to the terminal D is caused on the loop 31 in accordance with the impedance of the loop 31 and the above-described specific voltage V50. The biometric device 40 measures the current I51 at the endpoint C. In addition, biometric device 40 also provides a particular voltage V50 to endpoint E. At this time, a current I52 flowing from the end point E to the end point D is caused on the loop 32 in accordance with the impedance of the loop 32 and the specific voltage V50 described above. The biometric device 40 measures the current I52 at the endpoint E.

It is known in the art that according to Ohm's Law, for a conductor, the current flowing through a conductor is inversely proportional to the impedance of the conductor. Therefore, the currents I50, I51, and I52 calculated by the biometric device 40 can represent the impedance of the loop 30, the impedance of the loop 31, and the impedance of the loop 32, respectively. In other words, the biometric device 40 measures the impedance of the loop 30, the impedance of the loop 31, and the impedance of the loop 32 by measuring the currents I50, I51, and I52, respectively. After obtaining the currents I50, I51, and I52, the biometric device 40 obtains the code Code1 of the biosensor 1 according to the formula (1) and/or the formula (2), and according to the formula (1) and/or the formula (1) 2) To obtain the code 2 of the biosensor 1 . For example, in an embodiment, the biometric device 40 obtains the code Code1 of the biosensor 1 according to the formula (1), and obtains the code Code2 of the biosensor 1 according to the formula (2).

Where i50 represents the value of current I50, i51 represents the value of current I51, and i52 represents the value of current I52. The TRUNC( ) function converts a numeric value containing a decimal to an integer (that is, unconditionally rounding off the part after the decimal point).

For example, refer to Table 1, which is an exemplary example of the currents I50, I51, and I52 values i50, i51, and i52, the biosensor 1 code Code1, and the biosensor 1 code Code2, wherein Table 1 lists the codes Code1 and Code2 which are all calculated by the above formula (1).

As another example, refer to Table 2, which is another example of the currents I50, I51, and I52 values i50, i51, and i52, the biosensor 1 code Code1, and the biosensor 1 code Code2. For example, in Table 1, the code Code1 and the code Code2 are all calculated by the above formula (2).

In accordance with the above, the biosensor 1 of the present invention has three loops 30, 31, and 32 for defining two codes Code1 and Code2 representing the characteristics of the biosensor 1. When the user wants to measure the analyte of a biological sample through the biometric device 40, the user inserts the biosensor 1 into the slot 41 of the biometric device 40. At this time, the biometric device 40 can simultaneously measure the impedances of the three loops 30, 31, and 32, thereby obtaining the codes Code1 and Code2. The biometric device 40 sets at least one parameter of the measurement operation according to the codes Code1 and Code2, so that the biometric device 40 can perform the measurement operation on the glucose of the blood according to the parameter. In this way, the user does not need to input the code indicating the characteristics of the biosensor 1 into the biometric device 40 by input. In addition, since the circuit is disposed on one side of the biosensor 1, the user does not need to perform the operation of inserting the password card into the biometric device 40, and the insertion of the wrong password card is avoided.

The present invention has been disclosed in the above preferred embodiments, and is not intended to limit the scope of the present invention. Any one of ordinary skill in the art can make a few changes without departing from the spirit and scope of the invention. The scope of protection of the present invention is therefore defined by the scope of the appended claims.

1. . . Biosensor

4. . . Biometric system

10. . . Base

10a, 10b. . . Side of the base

11. . . Conductive layer

12. . . Biological reaction layer

20. . . Reaction zone

21, 22. . . electrode

30, 31, 32. . . Loop

40. . . Biometric device

41. . . Slot

42. . . Display panel

A, B, C, D, E. . . End point

GND. . . Ground potential

I50, I51, I52. . . Current

V50. . . Specific voltage

1 is a side view showing a biosensor according to an embodiment of the present invention;

Figure 2 shows a side of the base of the biosensor;

Figure 3A shows the other side of the base of the biosensor;

Figure 3B shows the code pattern on the side of Figure 3A;

Figure 4 shows a biometric system in accordance with an embodiment of the present invention;

Figures 5A and 5B show schematic diagrams of currents in various loops measured in a biometric system.

30, 31, 32. . . Loop

A, B, C, D, E. . . End point

Claims (20)

A biosensor for sensing a biological sample and having a first code indicating a characteristic of the biosensor and a second code, comprising:
a substrate, and a conductive layer disposed on the first side of the substrate, wherein the conductive layer comprises:
a first loop formed between a first end point and a second end point and having a first impedance;
a second loop formed between a third end point and a fourth end point and having a second impedance, wherein the second end point and the third end point are electrically insulated from each other; and a third a loop formed between the fourth end point and a fifth end point and having a third impedance;
The first impedance and the second or third impedance define the first code, and the first impedance and the second or third impedance define the second code. The biosensor as described in claim 1 further includes:
a biological reaction layer disposed in a biological reaction zone on one of the second sides of the substrate and having a chemical reagent,
Wherein the biological sample can be placed in the biological reaction zone; and wherein the second side is opposite the first side. The biosensor of claim 2, wherein the first code and the second code represent reaction characteristics of the chemical reagent. The biosensor of claim 2, wherein the biosensor senses glucose of the biological sample. The biosensor of claim 1, wherein the first loop, the second loop, and the third loop have substantially the same line width, and the first loop, the second loop, And determining, by the respective lengths of the third loops, the first impedance, the second impedance, and the third impedance. The biosensor of claim 1, wherein the first code and the second code represent manufacturing information of the biosensor. The biosensor of claim 1, wherein the first loop, the second loop, and the third loop of the conductive layer are disposed on the substrate by screen printing techniques. The biosensor of claim 1, wherein the biosensor is a blood glucose sensor. The biosensor of claim 1, wherein the first impedance and the second impedance define the first code, and the first impedance and the third impedance define the second code. A biometric system for sensing an analyte of a biological sample, comprising:
a biosensor having a first code indicating a characteristic of the biosensor and a second code, and comprising:
a substrate having a first side and a second side opposite the first side;
a biological reaction layer disposed in the biological reaction zone of the first side and having a chemical reagent, wherein the biological sample can be placed in the biological reaction zone;
a conductive layer disposed on the second side, wherein the conductive layer comprises:
a first loop formed between a first end point and a second end point and having a first impedance;
a second loop formed between a third end point and a fourth end point and having a second impedance, wherein the first loop and the second loop are electrically insulated from each other; and a third loop, Forming between the fourth end point and a fifth end point, and having a third impedance; and a biometric device, wherein the biosensor can be connected to the biometric device;
Wherein the biometric device measures the first impedance, the second impedance, and the third impedance, and calculates the first code according to the first impedance and the second or third impedance and according to the first The second code is calculated by the impedance and the second or third impedance; and wherein the biometric device performs a measurement operation on the analyte of the biological sample based on the first code and the second code. The biometric system of claim 10, wherein the first code and the second code represent reaction characteristics of the chemical reagent. The biometric system of claim 10, wherein the biosensor senses glucose of the biological sample. The biometric system of claim 10, wherein the first loop, the second loop, and the third loop have the same line width, and the first loop, the second loop, and The respective lengths of the third loop determine the first impedance, the second impedance, and the third impedance, respectively. The biometric system of claim 10, wherein the first code and the second code represent manufacturing information of the biosensor. The biometric system of claim 10, wherein the first loop, the second loop, and the third loop of the conductive layer are disposed on the substrate by screen printing techniques. For example, the biological measurement system described in claim 10,
Wherein, when the biometric device couples the second end point to a ground potential and provides a specific voltage to the first end point, the biometric measuring device measures one of the first end points Current to indicate the first impedance;
Wherein, when the biometric device couples the fourth end point to the ground potential and provides the specific voltage to the third end point, the biometric measuring device measures one of the second end points a current to indicate the second impedance; and wherein, when the biometric device couples the fourth terminal to the ground potential and provides the specific voltage to the fifth terminal, the biometric device measures A third current on the fifth terminal to represent the third impedance. The biometric system of claim 10, wherein when the biosensor is connected to the biometric device, the biometric device sets the biometric according to the first code and the second code At least one parameter of the measurement operation is measured to measure the analyte. The biometric measuring system of claim 10, wherein the biometric measuring device has a display screen for displaying a measurement result of the measuring operation on the analyte. The biological measurement system of claim 10, wherein the biological measurement system is a blood glucose measurement system. The biometric system of claim 10, wherein the biometric device calculates the first code according to the first impedance and the second impedance and according to the first impedance and the third impedance Calculate the second code.