TWI456064B - 非揮發性固態微生物代謝物之發酵生產 - Google Patents
非揮發性固態微生物代謝物之發酵生產 Download PDFInfo
- Publication number
- TWI456064B TWI456064B TW095132992A TW95132992A TWI456064B TW I456064 B TWI456064 B TW I456064B TW 095132992 A TW095132992 A TW 095132992A TW 95132992 A TW95132992 A TW 95132992A TW I456064 B TWI456064 B TW I456064B
- Authority
- TW
- Taiwan
- Prior art keywords
- weight
- fermentation broth
- carbon atoms
- metabolite
- volatile
- Prior art date
Links
- 239000002207 metabolite Substances 0.000 title claims 15
- 239000007787 solid Substances 0.000 title claims 12
- 230000000813 microbial effect Effects 0.000 title claims 4
- 238000012262 fermentative production Methods 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims 22
- 230000004151 fermentation Effects 0.000 claims 22
- 238000000034 method Methods 0.000 claims 22
- 239000007788 liquid Substances 0.000 claims 16
- 125000004432 carbon atom Chemical group C* 0.000 claims 14
- 229920002472 Starch Polymers 0.000 claims 13
- 235000019698 starch Nutrition 0.000 claims 13
- 239000008107 starch Substances 0.000 claims 13
- 239000000203 mixture Substances 0.000 claims 6
- 238000009472 formulation Methods 0.000 claims 5
- 239000000463 material Substances 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- 240000005384 Rhizopus oryzae Species 0.000 claims 4
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims 4
- 229940088598 enzyme Drugs 0.000 claims 4
- 150000002772 monosaccharides Chemical class 0.000 claims 4
- 241000193403 Clostridium Species 0.000 claims 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 3
- 125000001931 aliphatic group Chemical group 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 244000005700 microbiome Species 0.000 claims 3
- 239000011782 vitamin Substances 0.000 claims 3
- 235000013343 vitamin Nutrition 0.000 claims 3
- 229940088594 vitamin Drugs 0.000 claims 3
- 229930003231 vitamin Natural products 0.000 claims 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims 2
- 241000722955 Anaerobiospirillum Species 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 241000186660 Lactobacillus Species 0.000 claims 2
- 241000186429 Propionibacterium Species 0.000 claims 2
- 239000002671 adjuvant Substances 0.000 claims 2
- 102000004139 alpha-Amylases Human genes 0.000 claims 2
- 108090000637 alpha-Amylases Proteins 0.000 claims 2
- 229940024171 alpha-amylase Drugs 0.000 claims 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 claims 2
- -1 carotenoid Ketones Chemical class 0.000 claims 2
- 235000013339 cereals Nutrition 0.000 claims 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims 2
- 150000002016 disaccharides Chemical class 0.000 claims 2
- 238000000227 grinding Methods 0.000 claims 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 2
- 150000002576 ketones Chemical class 0.000 claims 2
- 229940039696 lactobacillus Drugs 0.000 claims 2
- 150000002763 monocarboxylic acids Chemical class 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108010011619 6-Phytase Proteins 0.000 claims 1
- 241000186426 Acidipropionibacterium acidipropionici Species 0.000 claims 1
- 241000606750 Actinobacillus Species 0.000 claims 1
- 241000948980 Actinobacillus succinogenes Species 0.000 claims 1
- 241000588986 Alcaligenes Species 0.000 claims 1
- 241001136167 Anaerotignum propionicum Species 0.000 claims 1
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000228245 Aspergillus niger Species 0.000 claims 1
- 241000193033 Azohydromonas lata Species 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 244000063299 Bacillus subtilis Species 0.000 claims 1
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
- 239000002028 Biomass Substances 0.000 claims 1
- 241000193401 Clostridium acetobutylicum Species 0.000 claims 1
- 241000193161 Clostridium formicaceticum Species 0.000 claims 1
- 241000186216 Corynebacterium Species 0.000 claims 1
- 241000186226 Corynebacterium glutamicum Species 0.000 claims 1
- 229920000858 Cyclodextrin Polymers 0.000 claims 1
- 241001465321 Eremothecium Species 0.000 claims 1
- 241001465328 Eremothecium gossypii Species 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000192601 Fischerella Species 0.000 claims 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 1
- 102100022624 Glucoamylase Human genes 0.000 claims 1
- 241001134654 Lactobacillus leichmannii Species 0.000 claims 1
- 241000186428 Propionibacterium freudenreichii Species 0.000 claims 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims 1
- 241000235527 Rhizopus Species 0.000 claims 1
- 241000209056 Secale Species 0.000 claims 1
- 235000007238 Secale cereale Nutrition 0.000 claims 1
- 235000019714 Triticale Nutrition 0.000 claims 1
- 235000021307 Triticum Nutrition 0.000 claims 1
- 241000209140 Triticum Species 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 235000019728 animal nutrition Nutrition 0.000 claims 1
- 150000001491 aromatic compounds Chemical class 0.000 claims 1
- 229920001222 biopolymer Polymers 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 235000014633 carbohydrates Nutrition 0.000 claims 1
- 235000021466 carotenoid Nutrition 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 229940097362 cyclodextrins Drugs 0.000 claims 1
- 235000013325 dietary fiber Nutrition 0.000 claims 1
- 150000002009 diols Chemical class 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000001879 gelation Methods 0.000 claims 1
- 150000002596 lactones Chemical class 0.000 claims 1
- 239000010985 leather Substances 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000007791 liquid phase Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- 125000003835 nucleoside group Chemical group 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 239000000123 paper Substances 0.000 claims 1
- 229940085127 phytase Drugs 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 235000019260 propionic acid Nutrition 0.000 claims 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- 229920006395 saturated elastomer Polymers 0.000 claims 1
- 235000003441 saturated fatty acids Nutrition 0.000 claims 1
- 150000004671 saturated fatty acids Chemical class 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 239000004753 textile Substances 0.000 claims 1
- 150000003628 tricarboxylic acids Chemical class 0.000 claims 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 claims 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 241000228158 x Triticosecale Species 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/10—Citrulline; Arginine; Ornithine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/12—Methionine; Cysteine; Cystine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/24—Proline; Hydroxyproline; Histidine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (24)
- 一種藉由以糖為基礎之微生物發酵作用生產至少一種非揮發性固態微生物代謝物之方法，其中該方法中生產所需代謝物之微生物菌株係使用具有以液體培養基總重量計之20%重量比以上單醣含量之含糖液體培養基培育，藉此獲得一發酵液，該發酵液含有所欲代謝物及用於生產含糖液體培養基之澱粉原料中之非澱粉成分，且隨後該含有該代謝物之發酵液之揮發性成分係自該發酵液移除，直至殘餘水分含量為以該等發酵液之固體成分總乾重計之0.2至20%重量比，而未預先分離掉該發酵液之不溶性成分，其中該含糖液體培養基係以包含以下之步驟製備：a1)製備研磨基料，其係藉由研磨選自穀粒之澱粉原料；及a2)在至少一種澱粉液化酶存在下，在水性液體中液化該研磨基料，繼之使用至少一種糖化酶進行糖化作用，藉以獲得具有20%重量比以上之單醣含量之含糖液體培養基，其中該含糖液體培養基亦包含至少50%重量比之該澱粉原料之非澱粉固體成分；其中，對於該液化目的而言，以所使用研磨基料之總重量計，至少40%重量比之研磨基料在該液化期間係連續性地或批次性地添加至該水性液體中。
- 如請求項1之方法，其包含a)如步驟a1)及a2)中所述，製備具有20%重量比以上之 單醣含量之含糖液體培養基，其中該含糖液體培養基亦包含該澱粉原料之非澱粉固體成分；b)在發酵中使用該含糖液體培養基，以便生產該(等)非揮發性代謝物；及c)藉由移除該發酵液之至少某些揮發性成分，從該發酵液中獲得該(等)非揮發性固態代謝物及該澱粉原料之至少部分的非澱粉固體成分。
- 如請求項1或2之方法，其中該研磨基料係在至少一種α-澱粉酶存在下在水性液體中液化，並隨後使用至少一種葡糖澱粉酶加以糖化。
- 如請求項3之方法，其中該至少一種α-澱粉酶之部分係在步驟a2)之液化期間添加至該水性液體中。
- 如請求項1或2之方法，其中該穀物係選自玉米、黑麥、黑小麥及小麥穀粒。
- 如請求項1或2之方法，其中步驟a1)之研磨期間所獲得之該研磨基料包含至少50%重量比之粗粉顆粒，該等粗粉顆粒具有100微米以上之粒度。
- 如請求項1或2之方法，其中該研磨基料在步驟a2)中之液化及糖化作用係在使該液體培養基之黏度不超過20Pas之方式進行。
- 如請求項1或2之方法，其中在該液化期間所添加之該研磨基料總量之至少25%重量比係在高於該研磨基料中所存在之澱粉之膠凝溫度的溫度下添加。
- 如請求項1或2之方法，其中具有30%重量比以上單醣含 量之含糖液體培養基係經製備。
- 如請求項1或2之方法，其中至少一種植酸酶係在該發酵步驟之前添加至該含糖液體培養基中。
- 如請求項1或2之方法，其中該(等)所生產之非揮發性代謝物係選自：有機單羧酸、二羧酸及三羧酸，其視情況具有附著於其之羥基且具有3至10個碳原子；蛋白型及非蛋白型胺基酸、嘌呤鹼基、嘧啶鹼基；核苷、核苷酸、脂質；飽和及不飽和脂肪酸；具有4至10個碳原子之二醇、具有3或3個以上羥基之更高官能度醇、具有至少4個碳原子之更長鏈醇、碳水化合物、芳族化合物、維生素、原維生素、輔因子、營養品、蛋白質、類胡蘿蔔素、具有3至10個碳原子之酮、內酯、生物聚合物及環糊精。
- 如請求項1或2之方法，其中該等所製備之非揮發性代謝物係選自酶、胺基酸、維生素、雙醣、具有3至10個碳原子之脂族單羧酸及二羧酸、具有3至10個碳原子之脂族羥基羧酸、具有3至10個碳原子之酮、具有4至10個碳原子之烷醇及具有3至10個碳原子之烷二醇。
- 如請求項1或2之方法，其中該等微生物係選自可生產至少一種以下代謝物之天然或重組微生物：酶、胺基酸、維生素、雙醣、具有3至10個碳原子之脂族單羧酸及二羧酸、具有3至10個碳原子之脂族羥基羧酸、具有3至10個碳原子之酮、具有4至10個碳原子之烷醇及具有3至10個碳原子之烷二醇。
- 如請求項13之方法，其中該等微生物係選自：棒狀桿菌屬(Corynebacterium)、芽胞桿菌屬(Bacillus)、棉阿舒囊黴屬(Ashbya)、埃希氏菌屬(Escherichia)、麯黴屬(Aspergillus)、產鹼桿菌屬(Alcaligenes)、放線桿菌屬(Actinobacillus)、厭氧螺菌屬(Anaerobiospirillum)、乳酸桿菌屬(Lactobacillus)、丙酸桿菌屬(Propionibacterium)、梭菌屬(Clostridium)及根黴屬(Rhizopus)，其中尤其為以下菌株：麩胺酸棒桿菌(Corynebacterium glutamicum)、枯草芽孢桿菌(Bacillus subtilis)、棉阿舒囊黴(Ashbya gossypii)、大腸桿菌(Escherichia coli)、黑麯黴(Aspergillus niger)、協腹產鹼桿菌(Alcaligenes latus)、產琥珀酸厭氧螺菌(Anaerobiospirillum succiniproducens)、琥珀酸放線桿菌(Actinobacillus succinogenes)、德氏乳酸桿菌(Lactobacillus delbrückii)、萊希曼氏乳桿菌(Lactobacillus leichmannii)、阿拉伯糖丙酸桿菌(Propionibacterium arabinosum)、Propionibacterium schermanii、費氏丙酸桿菌(Propionibacterium freudenreichii)、丙酸梭狀芽孢桿菌(Clostridium propionicum)、丙酮丁醇梭桿菌(Clostridium acetobutlicum)、Clostridium formicoaceticum、米根黴(Rhizopus oryzae)及少根根黴(Rhizopus arrhizus)。
- 如請求項1或2之方法，其中該發酵液中所存在之不超過30%重量比之固體係在移除該發酵液之揮發性成分之前 移除。
- 如請求項1或2之方法，其中該發酵液之液相係經移除而未預先分離掉該發酵液之不溶性成分，且該代謝物與該發酵液之所有不溶性成分的全部一起獲得。
- 如請求項1或2之方法，其中該至少一種非揮發性代謝物係以固態型式與所有不溶性成分的全部一起從該發酵液中獲得，而未預先移除該發酵液之不溶性成分。
- 如請求項1或2之方法，其中該發酵液之揮發性成分自該發酵液移除，直至殘餘水分含量為以該等固體成分總乾重計之0.2至20%重量比，較佳地為1至15%重量比，及特佳地為5至10%重量比。
- 如請求項1或2之方法，其中該發酵液係經噴霧乾燥、流化床乾燥或冷凍乾燥，以移除該等揮發性成分。
- 如請求項19之方法，其中一或多種乾燥佐劑係經使用。
- 一種代謝物之固體調配物，其可藉由如請求項1至20中任一項之方法獲得，其包含：A)>10至80%重量比之至少一種非揮發性代謝物；B)1至50%重量比之生物質量，其係來自生產該非揮發性代謝物之發酵作用；C)1至50%重量比之來自該發酵液之該澱粉原料之非澱粉固體成分；及D)以組份A、B及C之總重量計之0至400%重量比之習知調配佐劑；A、B與C重量份總和為100%重量比。
- 如請求項21之調配物，其包含以該調配物總重量計至少5%重量比之膳食纖維。
- 一種如請求項21或22之調配物之用途，其係用於人類或動物營養。
- 一種如請求項21或22之調配物之用途，其係用於處理紡織品、皮革、纖維素、紙或表面。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102005042541A DE102005042541A1 (de) | 2005-09-07 | 2005-09-07 | Fermentative Herstellung nichtflüchtiger mikrobieller Stoffwechselprodukte in fester Form |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW200745342A TW200745342A (en) | 2007-12-16 |
| TWI456064B true TWI456064B (zh) | 2014-10-11 |
Family
ID=37668444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW095132992A TWI456064B (zh) | 2005-09-07 | 2006-09-07 | 非揮發性固態微生物代謝物之發酵生產 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20090226571A1 (zh) |
| EP (1) | EP1926823A1 (zh) |
| JP (2) | JP5199094B2 (zh) |
| KR (1) | KR101388759B1 (zh) |
| CN (2) | CN101300359A (zh) |
| AR (1) | AR055154A1 (zh) |
| AU (1) | AU2006289083B2 (zh) |
| BR (1) | BRPI0615697A2 (zh) |
| CA (1) | CA2623588C (zh) |
| DE (1) | DE102005042541A1 (zh) |
| MX (1) | MX304773B (zh) |
| NO (1) | NO20080932L (zh) |
| RU (1) | RU2422531C9 (zh) |
| TW (1) | TWI456064B (zh) |
| WO (1) | WO2007028804A1 (zh) |
| ZA (1) | ZA200802925B (zh) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005056669A1 (de) | 2005-11-28 | 2007-05-31 | Basf Ag | Fermentative Herstellung organischer Verbindungen unter Einsatz Dextrin-haltiger Medien |
| DE102005056668A1 (de) | 2005-11-28 | 2007-05-31 | Basf Ag | Fermentative Herstellung organischer Verbindungen |
| DE102005056667A1 (de) | 2005-11-28 | 2007-05-31 | Basf Ag | Fermentative Herstellung organischer Verbindungen |
| CN101617055A (zh) * | 2007-01-23 | 2009-12-30 | 巴斯夫欧洲公司 | 通过对使用含多原子阴离子的离子液体从含木素纤维素的材料得到的纤维素进行酶促水解来制备葡萄糖的方法 |
| DE102007059299A1 (de) | 2007-05-16 | 2008-11-20 | Entex Rust & Mitschke Gmbh | Vorrichtung zur Verarbeitung von zu entgasenden Produkten |
| BRPI0813522A2 (pt) | 2007-07-06 | 2014-12-30 | Basf Se | Processos para a produção de uma solução de glicose aquosa a partir do milho, e par a produção de uma substância orgânica pela fermentação, solução de glicose, uso de uma solução de glicose, glúten de milho, uso de um glúten de milho, e, composição de gênero alimentício |
| MX323778B (es) * | 2008-04-14 | 2014-09-22 | Basf Se | Procedimiento para la preparacion de una solucion acuosa de glucosa. |
| CN102459138A (zh) | 2009-06-04 | 2012-05-16 | 基因组股份公司 | 分离发酵液成分的方法 |
| JP4725869B1 (ja) * | 2010-05-19 | 2011-07-13 | 聖子 佐藤 | バイオマスプラスチックの製造方法及びその成形品 |
| IL207945A0 (en) | 2010-09-02 | 2010-12-30 | Robert Jansen | Method for the production of carbohydrates |
| KR101335853B1 (ko) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| BR112014020201A8 (pt) | 2012-02-15 | 2017-07-11 | Gs Cleantech Corp | biopolímeros e processos para produzir os mesmos |
| AU2013262755B2 (en) * | 2012-05-17 | 2015-10-01 | Shell Internationale Research Maatschappij B.V. | Animal feed products and methods of making same |
| BR112014028022B1 (pt) | 2012-05-17 | 2021-11-16 | Shell Internationale Research Maatschappij B.V. | Método para a recuperação de um composto orgânico volátil a partir de um material de biomassa |
| JP6259449B2 (ja) | 2012-05-17 | 2018-01-10 | シエル・インターナシヨネイル・リサーチ・マーチヤツピイ・ベー・ウイＳｈｅｌｌ Ｉｎｔｅｒｎａｔｉｏｎａｌｅ Ｒｅｓｅａｒｃｈ Ｍａａｔｓｃｈａｐｐｉｊ Ｂｅｓｌｏｔｅｎ Ｖｅｎｎｏｏｔｓｈａｐ | バイオマス材料から揮発性有機化合物を生産する方法 |
| CN113248347B (zh) | 2013-03-15 | 2024-05-10 | 基因组股份公司 | 用于从发酵肉汤中获得1,4-丁二醇的方法和系统 |
| EP3200604B2 (de) | 2014-10-02 | 2025-05-07 | Evonik Operations GmbH | Verfahren zur herstellung eines futtermittels |
| WO2016050554A1 (de) | 2014-10-02 | 2016-04-07 | Evonik Degussa Gmbh | Pufas enthaltendes futtermittel mit hoher abriebfestigkeit und hoher wasserstabilität |
| CN107208122A (zh) * | 2014-12-02 | 2017-09-26 | 莱科威尔营养有限责任公司 | 全酒糟及其它生物质的提取物及其提取方法 |
| CN106520503A (zh) * | 2016-09-29 | 2017-03-22 | 陕西科技大学 | 一种发酵液储存方法 |
| EP3740084A4 (en) | 2018-01-18 | 2021-09-22 | Third Wave Bioactives, LLC | FERMENTATE COMPOSITIONS BASED ON FRUIT AND VEGETABLES AND THE PROCESS FOR THEIR PRODUCTION AND USE |
| CN108374025A (zh) * | 2018-06-02 | 2018-08-07 | 山东省同泰维润食品科技有限公司 | 一种丙酸制备工艺 |
| CN108796026A (zh) * | 2018-06-29 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | 赖氨酸的生产、提取以及纯化工艺 |
| CN110897032B (zh) * | 2019-11-19 | 2025-10-14 | 华南理工大学 | 一种发酵饲料蛋白及其制备方法与应用 |
| CN112359067B (zh) * | 2020-10-19 | 2022-09-09 | 安徽绿微康生物科技有限公司 | 发酵抗氧剂及其制备方法和应用 |
| CN112359068B (zh) * | 2020-10-19 | 2023-05-05 | 安徽绿微康生物科技有限公司 | 发酵防腐剂及其制备方法和应用 |
| CN112359069B (zh) * | 2020-10-20 | 2023-04-28 | 安徽绿微康生物科技有限公司 | 微生物发酵剂及其制备方法和应用 |
| CN112335810B (zh) * | 2020-10-20 | 2022-10-25 | 安徽绿微康生物科技有限公司 | 食用抗氧化剂及其制备方法和应用 |
| CN113373133B (zh) * | 2021-07-02 | 2023-10-20 | 广西乐酵生物科技有限公司 | 一种胶质玉米生产酒精的液化新工艺 |
| CN113699047B (zh) * | 2021-08-23 | 2023-09-29 | 延安大学 | 一种惰性支撑体粘附式发酵方法及其应用 |
| EP4265707A1 (en) * | 2022-04-21 | 2023-10-25 | Indian Oil Corporation Limited | A method for fatty acid alkyl ester synthesis and their extraction from oleaginous microbes |
| CN115305207B (zh) * | 2022-07-01 | 2024-04-12 | 厦门大学 | 一种全合成培养基、制备方法及其用于三孢布拉氏霉菌的培养方法 |
| CN115141292B (zh) * | 2022-07-18 | 2023-04-14 | 福州大学 | 全生物质自组装单组份膨胀型阻燃剂及其制备方法和应用 |
| KR102843049B1 (ko) * | 2023-09-01 | 2025-08-06 | 숙명여자대학교산학협력단 | 박테리아 셀룰로오스 다공성 구조체-곡물 바이오매스 복합체 및 이의 제조 방법 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321772A (zh) * | 2000-03-31 | 2001-11-14 | 味之素株式会社 | 木薯的直接糖化和使用糖类溶液的氨基酸发酵 |
| CN1422947A (zh) * | 2001-10-30 | 2003-06-11 | 罗凯脱兄弟公司 | 一种制备自给自足发酵培养基的方法 |
| WO2004113551A1 (en) * | 2003-06-25 | 2004-12-29 | Novozymes A/S | Process for the hydrolysis of starch |
| TW200613557A (en) * | 2004-05-28 | 2006-05-01 | Basf Ag | Fermentative production of fine chemicals |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1691965A (en) * | 1925-11-03 | 1928-11-20 | Rowntree And Company Ltd | Process for the production of citric acid |
| US2053769A (en) * | 1933-08-15 | 1936-09-08 | Dreyfus Henry | Removal of volatile compounds from a fermenting medium |
| JPS536236B1 (zh) * | 1969-02-03 | 1978-03-06 | ||
| JPS5938B2 (ja) * | 1980-06-03 | 1984-01-05 | 味の素株式会社 | 穀類澱粉の直接糖化方法 |
| DE3731293A1 (de) * | 1987-09-17 | 1989-04-06 | Gfv Pfeil Hoch 2 Pfeil Hoch Ge | Verfahren und vorrichtung zum aufschluss und abbau von staerke und anderen kohlehydraten in hoher konzentration |
| US5177008A (en) * | 1987-12-22 | 1993-01-05 | Kampen Willem H | Process for manufacturing ethanol and for recovering glycerol, succinic acid, lactic acid, betaine, potassium sulfate, and free flowing distiller's dry grain and solubles or a solid fertilizer therefrom |
| KR19980702782A (ko) * | 1995-03-09 | 1998-08-05 | 혼 마가렛 에이. | 녹말 액화 방법 |
| US6479700B2 (en) * | 2000-03-29 | 2002-11-12 | Archer-Daniels-Midland Company | Method for separating a basic amino acid from fermentation broth |
| JP2001309751A (ja) * | 2000-05-02 | 2001-11-06 | Ajinomoto Co Inc | 飼料用添加物 |
| DE60038560T2 (de) * | 2000-10-27 | 2009-06-25 | Ajinomoto Co., Inc. | Verfahren zur fermentativen Herstellung von L-Arginin |
| FR2816321B1 (fr) * | 2000-11-09 | 2003-01-24 | Roquette Freres | Procede de preparation d'un milieu de fermentation a partir d'une matiere premiere renouvelable |
| EP1304043A1 (en) * | 2001-10-19 | 2003-04-23 | Societe Des Produits Nestle S.A. | Liquefacion of food material |
| US20040063184A1 (en) * | 2002-09-26 | 2004-04-01 | Novozymes North America, Inc. | Fermentation processes and compositions |
| CN1415755A (zh) * | 2002-11-27 | 2003-05-07 | 郭冰 | 一种用稻谷发酵生产柠檬酸的新方法 |
| US7981639B2 (en) * | 2003-02-19 | 2011-07-19 | Academia Sinica | Starch-derived products |
-
2005
- 2005-09-07 DE DE102005042541A patent/DE102005042541A1/de not_active Withdrawn
-
2006
- 2006-09-06 KR KR1020087008227A patent/KR101388759B1/ko not_active Expired - Fee Related
- 2006-09-06 CN CNA2006800413493A patent/CN101300359A/zh active Pending
- 2006-09-06 EP EP06793261A patent/EP1926823A1/de not_active Withdrawn
- 2006-09-06 BR BRPI0615697-5A patent/BRPI0615697A2/pt not_active Application Discontinuation
- 2006-09-06 RU RU2008112954/10A patent/RU2422531C9/ru not_active IP Right Cessation
- 2006-09-06 JP JP2008529627A patent/JP5199094B2/ja not_active Expired - Fee Related
- 2006-09-06 CN CN201510382204.0A patent/CN104911213A/zh active Pending
- 2006-09-06 WO PCT/EP2006/066057 patent/WO2007028804A1/de not_active Ceased
- 2006-09-06 US US11/991,515 patent/US20090226571A1/en not_active Abandoned
- 2006-09-06 CA CA2623588A patent/CA2623588C/en not_active Expired - Fee Related
- 2006-09-06 AU AU2006289083A patent/AU2006289083B2/en not_active Ceased
- 2006-09-07 AR ARP060103891A patent/AR055154A1/es not_active Application Discontinuation
- 2006-09-07 TW TW095132992A patent/TWI456064B/zh not_active IP Right Cessation
-
2008
- 2008-02-18 MX MXPA08002316 patent/MX304773B/es unknown
- 2008-02-25 NO NO20080932A patent/NO20080932L/no not_active Application Discontinuation
- 2008-04-03 ZA ZA200802925A patent/ZA200802925B/xx unknown
-
2012
- 2012-10-05 JP JP2012223174A patent/JP2013048625A/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321772A (zh) * | 2000-03-31 | 2001-11-14 | 味之素株式会社 | 木薯的直接糖化和使用糖类溶液的氨基酸发酵 |
| CN1422947A (zh) * | 2001-10-30 | 2003-06-11 | 罗凯脱兄弟公司 | 一种制备自给自足发酵培养基的方法 |
| WO2004113551A1 (en) * | 2003-06-25 | 2004-12-29 | Novozymes A/S | Process for the hydrolysis of starch |
| TW200613557A (en) * | 2004-05-28 | 2006-05-01 | Basf Ag | Fermentative production of fine chemicals |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2422531C9 (ru) | 2012-07-27 |
| US20090226571A1 (en) | 2009-09-10 |
| JP2009506783A (ja) | 2009-02-19 |
| KR20080052652A (ko) | 2008-06-11 |
| AR055154A1 (es) | 2007-08-08 |
| JP5199094B2 (ja) | 2013-05-15 |
| MX304773B (es) | 2012-10-31 |
| CN101300359A (zh) | 2008-11-05 |
| CA2623588C (en) | 2016-06-28 |
| RU2008112954A (ru) | 2009-10-20 |
| AU2006289083B2 (en) | 2011-08-25 |
| KR101388759B1 (ko) | 2014-04-28 |
| RU2422531C2 (ru) | 2011-06-27 |
| NO20080932L (no) | 2008-06-03 |
| TW200745342A (en) | 2007-12-16 |
| MX2008002316A (es) | 2008-05-29 |
| EP1926823A1 (de) | 2008-06-04 |
| AU2006289083A1 (en) | 2007-03-15 |
| BRPI0615697A2 (pt) | 2012-12-18 |
| WO2007028804A1 (de) | 2007-03-15 |
| ZA200802925B (en) | 2009-06-24 |
| CA2623588A1 (en) | 2007-03-15 |
| DE102005042541A1 (de) | 2007-03-08 |
| CN104911213A (zh) | 2015-09-16 |
| JP2013048625A (ja) | 2013-03-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI456064B (zh) | 非揮發性固態微生物代謝物之發酵生產 | |
| US8293504B2 (en) | Method for the production of an aqueous glucose solution | |
| US8785154B2 (en) | Method for manufacturing an aqueous glucose solution from plants of the Triticeae species | |
| CN101313073B (zh) | 发酵产生有机化合物 | |
| CN101313076B (zh) | 发酵产生有机化合物 | |
| RU2008126155A (ru) | Ферментативное получение органических соединений с использованием декстринсодержащих сред | |
| JP2003164275A (ja) | 完全発酵培地の調製方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Annulment or lapse of patent due to non-payment of fees |