TWI451285B - Genetic code management system and method thereof - Google Patents

Description

Species genetic code management system and method

The invention relates to a method for managing a gene of a species and a system thereof, in particular to a method and a system for utilizing a code to manage a gene of a species.

Biotechnology is becoming more and more complete, and the work of sequencing with genes is becoming more and more complete. The research on the species of the earth has become very hot and important. In fact, it has a decisive influence on all human beings, the continuation of species, and disease control.

In order to study species cells, tissues, etc., relevant biological research institutions often need to purchase the required gene fragments of the species with institutions that specialize in the sequencing of the gene fragments of the species. However, the management of gene fragments is quite rough at present. In the process of purchasing the program, the protection of the gene fragments of the species is insufficient. The purchaser can copy the gene fragments at will, and it is easy for the institutions engaged in gene sequencing to suffer economic losses.

In order to solve the technical problem that the prior art has insufficient information protection for genetic sequencing and incurs economic loss, the present invention uses an algorithm to compile the gene fragments into a code pattern and pass the authentication mechanism of multiple levels. The process is safer.

The invention provides a method for managing a genetic code of a species, the method comprising: reading a genetic code sequence of a species; selecting a control parameter according to the length information of the genetic code sequence; converting the genetic code sequence into a binary by a table lookup conversion method Sequence; performing a sequence rearrangement calculation of the binary sequence to form a cellular automaton sequence; selecting a plurality of cellular automaton sequences to be combined to form a cellular automaton sequence array having a selected number of rows; and arranging the cellular automaton sequence array The state of each bit (0 or 1) is displayed as a black or white pixel, respectively, so that the cellular automaton sequence array can be further represented as a gene barcode pattern.

Wherein, the step of converting the gene cryptographic sequence into a binary sequence by a look-up table conversion method converts the gene cryptographic sequence formed by the arrangement of the amino acid into a binary sequence through the following table.

Wherein, the sequence rearrangement calculus can be expressed by the following formula:

D(i,j)=F[D(i-1,j-1), D(i-1,1), D(i-1,j+1)],if 1≦j<S-1; 1≦i<n

D(i,0)=F[D(i-1,S-1), D(i-1,0), D(i-1,1)], if j=0;1≦i<n

D(i,S-1)=F[D(i-1,S-2), D(i-1,S-1), D(i-1,0)], if j=S-1; 1≦i<n

Where D(i,j) represents a state value of 0 or 1 of a certain (i,j) position of the array of cellular automata after conversion; i is the selected number of rows; S represents the length of the binary sequence; F refers to the regular function of the sequence rearrangement calculus.

Wherein, the rule of the function F of the sequence rearrangement calculation is the following table:

The invention further provides a species genetic code management system, comprising a species genetic management center and a plurality of client computers for transmitting information through the internet and the genetic management center of the species, wherein: the genetic management center of the species comprises a system host And a radio frequency identification tag reader, a plurality of portable radio frequency memory devices and a gene bar code storage cabinet respectively connected to the system host; the portable radio frequency memory device is a portable RF tag The memory device is electrically separably connected to the host of the system and is read and recognized by the RFID tag reader, so that the host of the system can access the information of the portable radio frequency memory device; the gene barcode storage of the species The cabinet comprises a cabinet body, a plurality of drawers movably mounted on the body, and a plurality of electromagnetic lock control modules, wherein: the cabinet body comprises a plurality of drawer spaces, and the drawer spaces are recessed in the cabinet body in an array form Surface, each drawer space is separated by a space structure; each drawer is placed in one of the cabinet bodies In the drawer space, a portable radio frequency memory device is placed in a drawer, and the portable memory device stores a gene code and a gene barcode pattern; and each of the spacer structures has one electromagnetic lock relative to each drawer. a control module, the electromagnetic lock control module controls a position state of the corresponding drawer.

Wherein, each of the electromagnetic lock control modules includes an electromagnet and an elastic movable pin, and the electromagnet is controlled by the main body of the system to generate or cancel a magnetic attraction; the elastic movable pin is movably disposed in the spacing structure, In a relaxed state, the electromagnet is to release the magnetic force, so that one of the latches of the elastic movable latch is snapped against the outer surface of the drawer, and the elastic movable latch is attracted by the electromagnet and the drawer The bottom surface is separated.

The gene management center further includes an input module, a display module and a monitoring camera module, wherein the input module and the display module are human-machine input devices for controlling the host of the system; The group is monitored by the host of the system for the genetic barcode storage cabinet of the species.

Wherein, the genetic barcode storage cabinet of the species comprises a plurality of indicating units respectively mounted on the surface of each drawer, and the indicating unit may be a light emitting component.

The gene barcode storage cabinet of the species comprises an environmental parameter control module, which controls the temperature and humidity inside the cabinet body.

It can be seen from the foregoing that the present invention not only provides a method for forming a gene barcode pattern by gene fragment cryptography, but also has a preliminary but complete protection of the gene fragment cipher, and further proposes a complete storage cabinet and related programs, so that the overall gene fragment cipher is improved. The protection of the technology completely solves the problems of the existing technology and achieves the technical effect of safety use.

Please refer to the first figure and the second figure, which are preferred embodiments of the species gene cryptography management system of the present invention, comprising a species gene management center 10 and transmitting information to the species gene management center 10 via the Internet. a plurality of client computers 50, the species gene management center 10 includes a system host 12 and an input module 13 connected to the system host 12, a display module 14, a monitoring camera module 15, and a radio frequency identification tag. A receiver 16 (RFID), a plurality of portable RF memory devices 17 and a species gene barcode storage cabinet 18.

The system host 12 is a computer that can quickly process network information, program operations, algorithm calculations, etc., and can include a processor, a memory, and the like that are responsible for performing the aforementioned operations. The input module 13 and the display module 14 are human-machine input devices for controlling the system host 12.

The surveillance camera module 15 is monitored by the system host 12 to monitor the environment surrounding the genetic bar code storage cabinet 18.

The RFID tag reader 16 is a reader that reads a Radio Frequency Identification Tag (RFID), and the RFID tag reader 16 transmits the result of reading the RFID tag to the system host 12.

The portable radio frequency memory device 17 is a portable memory device that is combined with a radio frequency identification tag. The portable memory device is a device that can store electronic information, and is electrically connected to the system host 12 through a USB interface or the like. After being connected, the system host 12 can access the information of the portable radio frequency memory device 17. When the system host 12 reads the portable RF memory device 17, it controls the RFID tag reader 16 to first determine whether the RFID tag is correct or required for the portable RF memory device 17, if it is determined For the portable radio frequency memory device 17 required, the system host 12 can access the data inside the portable radio frequency memory device 17.

Please refer to the third figure and the fourth A and B diagrams. The gene barcode storage cabinet 18 includes a cabinet body 181, a plurality of drawers 182 movably mounted on the body 181, and a plurality of surfaces respectively mounted on the drawers 182. The indicating unit 183, the plurality of electromagnetic lock control modules 184 and an environmental parameter control module 185.

The cabinet body 181 includes a plurality of drawer spaces, and the drawer spaces may be recessed in an array on the surface of the cabinet body 181. Each drawer space may be separated by a spacing structure 1814.

Each of the drawers 182 is disposed in a drawer space of the cabinet body 181. One of the drawers 182 is externally provided with the indicating unit 183. The indicating unit may be a Light Emitting Diode (LED). A similar light-emitting element is used as a state in which the drawer 182 is displayed, such as on, off, malfunction, and the like.

In order to allow each drawer 182 to be automatically opened or closed by control, each of the spacer structures 1814 of the cabinet body 181 can be provided with one electromagnetic lock control module 184 with respect to each drawer 182. The electromagnetic lock control module The 184 includes an electromagnet 1842 and an elastic movable pin 1844. The electromagnet 1842 is controlled by the system host 12 to generate or cancel a magnetic attraction. The elastic movable pin 1844 is movably disposed in the spacer structure 1814 when in a relaxed state. The electromagnet 1842 is configured to release the magnetic attraction force, so that one of the latching ends of the elastic movable latch 1844 is locked against the outer surface of the drawer 182, so that the drawer 182 can be restrained within the cabinet body 181. An inverted concave structure is protruded from the bottom of one of the drawers 182 of the embodiment. When the elastic movable latch 1844 is in the relaxed state, the inverted concave structure is inserted, thereby restricting the activity of the drawer 182 and being placed inside the cabinet body 181; When the elastic movable pin 1844 is attracted by the electromagnet 1842 to be separated from the bottom surface of the drawer 182 (in this embodiment, the inverted concave structure is removed), By drawer 182 is mounted on the inside of the cabinet main body 181 of a thrust spring of 1812 Release locally to the main body cabinet 181.

The environmental parameter control module 185 controls the temperature and humidity inside the cabinet body 181.

In order to study and analyze the characteristics of a species, it is necessary to first understand the genetic composition of the species. A gene is a group of meaningful genetic units. In the human body, for example, the cells of the human body have cell membranes, cytoplasm, nucleus and other organelles. There are chromosomes in the nucleus, which are substances composed of some proteins and DNA, carrying genetic codes. . When a species completes genetic sequencing, it can know its genetic code and can be expressed as a protein sequence. In the case of human cells, the protein is composed of about 20 amino acids, so that a gene code can be converted into the protein sequence. Therefore, the genetic code of the species can be stored in the form of a string fragment (arrangement of amino acids) after sequencing of the gene.

Please refer to the fifth figure. In order to properly keep the genetic code secret and preserved, the system host 12 can execute a genetic barcode calculation program. The genetic barcode calculation program includes the following steps:

(511) Input sequence: reading the genetic code sequence of a species.

(512) converting the DNA sequence into a protein sequence: converting the gene code sequence into a protein sequence, and converting the plurality of amino acids of the protein sequence into a binary representation by a look-up table conversion method, The protein sequence forms a binary sequence. The look-up table conversion method of the embodiment is performed by converting the following list 1:

(515) performing an image generation process in a binary sequence: after completing the conversion of the binary sequence, performing a sequence rearrangement calculation on the binary sequence, wherein the sequence arrangement algorithm takes every three phases of the binary sequence The adjacent bits are subjected to iterative conversion according to the values in Table 2 below. When the iterative conversion of every three adjacent bits is completed, one bit is moved to the right to continue the iterative process, and finally a new cell is generated. Automaton sequence. After the sequence rearrangement calculation is completed, a plurality of binary sequences are selected to form a selected number of rows side by side, and then a cellular automaton sequence array is formed. This step can be simplified to the following formula for the formula:

D(i,j)=F[D(i-1,j-1), D(i-1,1), D(i-1,j+1)],if 1≦j<S-1; 1≦i<n

D(i,0)=F[D(i-1,S-1), D(i-1,0), D(i-1,1)], if j=0;1≦i<n

D(i,S-1)=F[D(i-1,S-2), D(i-1,S-1), D(i-1,0)], if j=S-1; 1≦i<n

Where D(i,j) represents the state value of 0 or 1 of a certain (i,j) position of the cellular automaton sequence after iteration; i is the selected number of rows; S represents the length of the binary sequence; F refers to the regular function of the sequence rearrangement calculus.

The relationship between the sequences is illustrated by the actual example EX1:

(1) The protein sequence is: QRY...

(2) The binary sequence after the table lookup conversion is: 00101 00111 01100...

(3) The first 1, 2, and 3 bits "001" are as shown in the above table, the second is 0; the 2nd, 3, and 4 bits "010" are replaced by 1; the 3rd, 4th, and 5th, "101" is the iteration. 0... and so on; until the last bit cannot be iterated due to the lack of two bits, then the first and second bits are re-formed to form a binary string and then the table is inverted. The cellular automaton sequence of the binary is obtained.

(517) generating a bar code graphic: displaying a state (0 or 1) of each bit of the cellular automaton sequence array in a black or white pixel, respectively, so that the cell automaton sequence array can be further represented as a gene bar code graphic The bar code graphic is a black and white graphic.

Please refer to the sixth to tenth drawings. The host 12 of the system can execute a gene bar code pattern creation and storage process, automatically read the genetic code of the species and graphically store the barcode, and store the code in the memory. The storage process steps can include:

(611) reading the DNA fragment of the species DNA gene fragment, and reading the sequence of the DNA gene fragment of each species by a species gene fragment cryptographic database.

(612) Parsing the DNA gene fragment string to generate a control parameter: generating a control parameter representing the length according to characteristics such as the length of the read DNA gene fragment cipher string, such as the i, j, S parameters of the foregoing example.

(613) Performing a DNA gene barcode algorithm subroutine: performing the gene barcode calculation program to convert the gene fragment password string into the gene barcode pattern.

(615) Giving gene fragment coding and form production: the host computer 12 numbers the completed barcode pattern of the gene, and creates a form according to the species corresponding to each gene barcode pattern and the gene fragment, wherein the form can be As shown in the seventh figure, the information contained in the form may include the barcode pattern of the gene, the function description of the gene fragment corresponding to the barcode pattern of the gene, the host living environment of the corresponding species, the regionality, the time of completing the gene sequencing, and the sample. Sampling location and related documents.

(616) storing the gene barcode to the portable radio frequency memory device: the system host 12 stores the completed form and the gene barcode pattern into the portable radio frequency memory device 17.

(617) storing the form and the gene barcode on the web server: the system host 12 stores the completed form and the gene barcode pattern into a gene barcode image database of one of the network servers.

In order to allow the research user using the gene fragment to easily purchase the desired gene fragment code, the system host 12 can provide a gene segment procurement program, the steps of which include:

(71) User Endpoint Reading Spectral Gene Form: The user connects to a webpage provided by the web server through a network, and the webpage lists various forms for completing the genetic sequencing of the species for the user to click.

(72) Click on the gene fragment form, number or barcode to be purchased by the user: accept the form or form number required by the user endpoint or the gene barcode pattern corresponding to the form.

(73) Filling in personal data and usage: The web server accepts the user's input of personal data and the use of gene fragments for the purchase of the species.

(74) Complete the purchase service payment process: The web server provides an optional and payment page for the user to make payment and purchase processes.

(75) transmitting the user profile payment status and purchasing the gene barcode graphic to the species gene management center: the network server sends the user's personal data, payment status and the selected gene barcode graphic to the system host 12 Gene barcode graphic database.

(76) The transmission is successful: the network server determines whether the step (75) is successful. If it is not successful, the step (71) is re-executed.

After the system host 12 receives the order information transmitted by the network server, it can execute a gene barcode drawing acquisition program to output the gene barcode pattern. The steps of the gene barcode drawing acquisition program include:

(81) Reading the personal information of the user terminal, the payment status and the required gene segment information from the database: the system host 12 obtains the personal information of the user terminal, the payment status and the information of the desired gene segment.

(82) Personal data and usage review for the client: The system host 12 reads a review result notification of a message input by the administrator to the user.

(83) Pass: the system host 12 determines whether the review result notification is a pass, and if the determination result is yes, proceed to the next step, otherwise, the password is denied (831) step, and then the user is notified. Refund (832) steps.

(84) Comparing the user side to purchase the gene fragment and the species attribute: the system host 12 reads the gene fragment purchased by the user and finds the corresponding species attribute.

(85) The selected species cabinet number and the species gene drawer are transmitted: the system host 12 finds the position and number of a certain drawer 182 placed in the portable RF memory device 17 storing the gene fragment and the gene barcode pattern.

(86) Waiting for processing: After receiving the license signal from one of the second administrators, the system host 12 performs the next step.

(87) Whether to accept the password file returned by the second administrator: the system host 12 determines whether the decryption password of one of the second administrators has been received, and if the determination result is no, then a urging message (871) is performed, Otherwise, the step of transmitting the gene barcode pattern and the decryption password to the client (872) is performed.

The foregoing second administrator may be a terminal or administrator responsible for storing the password. After accepting the data of the first manager of the system host 12, the correct portable radio frequency memory device 17 may be taken out in the following steps. Linked to the system host 12:

(91) Accepting order information: The second manager accepts the user's order information.

(92) Obtaining the gene storage cabinet number: from the order information, the desired gene fragment is obtained, and the location of the portable radio frequency memory device 17 storing the gene fragment and the gene barcode pattern is obtained by the database.

(93) Driving the electromagnetic lock control module: the system host 12 controls the electromagnetic lock control module 184 corresponding to a certain drawer 182, and the drawer 182 is pushed out of the cabinet body 181.

(94) Confirming the ON state of the surveillance camera module: The system host 12 confirms that the connected photography module 15 is in an open state.

(95) The indicating unit of the drawer is opened: the system main unit 12 opens the indicating unit 183 corresponding to the drawer 182 that is selected to be opened.

(96) Connecting the portable memory device: The system host 12 accepts connection with the portable memory device 17.

(97) The RFID reader performs identification: the system host 12 first determines whether the connected portable memory device 17 is correct by using the RFID tag reader 16, and if the determination result is yes, proceed to the next step. Otherwise, the error message (98) is transmitted and the process returns to step (92).

(99) reading the portable memory device 99: the system host 12 normally reads the internal information corresponding to the user's subscription content stored by the portable memory device 99, such as a gene barcode pattern and a corresponding gene fragment password, and Download the gene fragment password according to the gene barcode purchased by the user.

It can be seen from the foregoing that the present embodiment not only provides a method for forming a gene barcode pattern into a gene fragment cipher, but also has a preliminary but complete protection of the gene fragment cipher, and further proposes a complete storage cabinet and related programs, so that the entire gene fragment cipher is obtained. Perfect protection, completely solve the problems of existing technologies and achieve the technical effects of safe use.

10. . . Species Genetic Management Center

12. . . System host

13. . . Input module

14. . . Display module

15. . . Surveillance camera module

16. . . RFID tag reader

17. . . Portable RF memory device

18. . . Species gene barcode storage cabinet

181. . . Cabinet body

1812. . . spring

182. . . drawer

183. . . Indicating unit

184. . . Electromagnetic lock control module

1842. . . Electromagnet

1844. . . Flexible active latch

185. . . Environmental parameter control module

50. . . Client computer

The first figure is a schematic diagram of a system according to a preferred embodiment of the present invention.

The second figure is a system block diagram of a species gene management center according to a preferred embodiment of the present invention.

The third figure is a partial enlarged schematic view of a gene barcode storage cabinet of one species of the present invention.

The fourth figure is a schematic diagram of a drawer switch state of the present invention.

The fifth figure is a flow chart of a gene barcode calculation program according to a preferred embodiment of the present invention.

Figure 6 is a flow chart showing the production and storage of a gene barcode pattern according to a preferred embodiment of the present invention.

Figure 7 is a biological data form in accordance with a preferred embodiment of the present invention.

Figure 8 is a flow chart of a gene fragment procurement procedure according to a preferred embodiment of the present invention

The ninth figure is a flow chart of the process of obtaining the barcode of the gene barcode according to the preferred embodiment of the present invention.

FIG. 10 is a flow chart of a portable memory device reading program according to a preferred embodiment of the present invention;

10. . . Species Genetic Management Center

50. . . Client computer

Claims (6)

A genetic code management system for a species, comprising a species genetic management center and a plurality of client computers for transmitting information through the internet and the genetic management center of the species, wherein: the genetic management center of the species comprises a system host and respectively connected to the The system is a radio frequency identification tag reader, a plurality of portable radio frequency memory devices and a species gene bar code storage cabinet; the portable radio frequency memory device is a portable memory device combined with a radio frequency identification tag, The system is detachably connected to the host computer and is read and recognized by the RFID tag reader, so that the system host can access the information of the portable RF memory device; the genetic barcode storage cabinet of the species includes a cabinet The main body and the plurality of activities are installed on the drawer of the main body and the plurality of electromagnetic lock control modules, wherein: the cabinet body comprises a plurality of drawer spaces, and the drawer spaces are recessed on the surface of the cabinet body in an array, each of The drawer space is separated by a space structure; each drawer is placed in a drawer space of the cabinet body, A portable RF memory device is placed in a drawer, and the portable memory device stores a gene code and a gene barcode pattern; each of the spacer structures is provided with an electromagnetic lock control module for each drawer, the electromagnetic The lock control module controls the position state of the corresponding drawer; wherein the genetic code of the species and the barcode pattern of the gene are obtained by a genetic code management method of a species, and the step comprises: reading a genetic code sequence of a species; Selecting a control parameter for the length information of the password sequence; Converting the genetic code sequence into a binary sequence by a look-up table conversion method; performing a sequence rearrangement calculation on the binary sequence to form a cellular automaton sequence; selecting a plurality of cellular automaton sequences to be merged to form a selected row number a cellular automaton sequence array; and displaying the state (0 or 1) of each bit of the cellular automaton sequence array in a black or white pixel, respectively, such that the cellular automaton sequence array can be further represented as the gene barcode Graphics. For example, in the species gene cryptography management system described in claim 1, the gene cryptographic sequence is converted into a binary sequence by a look-up table conversion method, and the gene formed by aligning the amino acid is arranged through the following table. Converting the password sequence into a binary sequence; The sequence rearrangement calculus can be expressed by the following formula: D(i,j)=F[D(i-1,j-1), D(i-1,1),D(i-1,j+1)] , if 1≦j<S-1;1≦i<n D(i,0)=F[D(i-1,S-1), D(i-1,0), D(i-1, 1)], if j=0;1≦i<n D(i,S-1)=F[D(i-1,S-2), D(i-1,S-1),D(i -1,0)], if j=S-1; 1≦i<n where D(i,j) represents 0 or 1 of a certain (i,j) position of the array of cellular automaton sequences after conversion. State value; i is the selected number of rows; S represents the length of the binary sequence; and F refers to the regular function of the sequence rearrangement calculus; and the rule of the function F of the sequence rearrangement calculus is the following table: The gene cryptography management system of claim 2, wherein each of the electromagnetic lock control modules comprises an electromagnet and an elastic movable latch, and the electromagnet is controlled by the host of the system to generate or cancel a magnetic attraction; The elastic movable latching action is disposed in the spacing structure. When in a relaxed state, the electromagnet is used to release the magnetic attraction force, so that one of the latching ends of the elastic movable latch is locked against the outer surface of the drawer, and vice versa. The elastic movable latch is attracted to the electromagnet to be separated from the bottom surface of the drawer. The gene management system of the species according to claim 3, wherein the genetic management center further comprises an input module, a display module and a monitoring camera module, wherein the input module and the display module are controlled The human-machine input device of the system host; the surveillance camera module is monitored by the host of the system to monitor the genetic barcode storage cabinet of the species. The genetic code storage system of the species according to claim 4, wherein the genetic bar code storage cabinet comprises a plurality of indicating units respectively mounted on the surface of each drawer, and the indicating unit may be a light emitting element. For example, the genetic code management system of the species described in claim 8 of the patent scope, the genetic barcode storage cabinet of the species comprises an environmental parameter control module, Control the temperature and humidity inside the cabinet body.