TWI378772B - The formulation of microemulsion of plant protectant, spreader, nematicide and agricultural chemical on control of plant root-knot nematode diseases - Google Patents

Description

1378772 VI. Description of the invention: 7 [Technical field to which the invention pertains] The present invention relates to an oil-in-water (o-in-water, o) by means of a microemulsification technique in which cinnamon oil is assisted by bitter tea saponin and ethanol. /w) state, a microemulsion used to control crop root nodule nematodes. [Prior Art] Taiwan is located in a tropical and subtropical climate zone. It is located in a hot and humid environment all year round. It is very easy to produce or spread a variety of crop pests and diseases, and seriously affect the output and quality of agricultural products. Plant pathogenic nematodes have become the key pathogens for continuous cropping in many specialized areas of fruit trees, flowers and fruits and vegetables in Taiwan, especially in horticultural facilities. The use of highly toxic nematicidal agents in mountain fields has great harm to the environment and ecology, and there are residues of agricultural products. So far, the world's commercially available nematicides are expected to be largely banned in the next fifteen years. 'Non-Pesticide Control will be The only way to do it.

/, from the machine to the Han, gradually toward the ° sustainable agriculture "management" hopes to be able to work by wow energy / through the ecological balance of farming ideas, using a variety of cultivation management measures, with the farmers | 4 g - The morning morning crops are recycled and reused to produce agricultural products without agricultural residues. In the production of organic agricultural products, the problems that must be overcome immediately must be overcome. The management of 丄^ harm is based on R&D #. Research on the prevention of metallurgy ^ Queen and effective non-pesticide prevention and control of chemical pesticides to protect the human Lord, and gradually reduce the sustainable operation (4,7,14,17 2〇>. Ecological balance and agriculture Seeking high-yield and going from ^大$ using chemical fertilizers, 荦 门 , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ". All of them are faced by Taiwan's agricultural production, and neighboring countries implement sustainable agriculture' to suppress their occurrence. Too, use the ecological characteristics of pests and diseases to use insect nets, sexual pheromones, light, silver plates, dots Abusing, buying, microbial, sealing, and other things to catch people, plants, etc., or using "*plants to catch cockroaches, block, induce 5|disruption, mix things, etc. to suppress the occurrence of pests and diseases (1,2,91819 礼$—avoiding the country’s organic farming management method to solve this problem came into being, and it’s been done in China for more than ten years. Limit, it is due to the economic benefits of farmers Level D丨_ μ J 3, the main reason for the lack of sustainable use of fertilizers and pesticides in pursuit of high yield (4, ~ other organic fertilizer quality instability, prevention and control of plant nematode disease natural drug development is also the main reason One (7·14, called. Therefore 'production quality and cost-effective natural bio-protective preparations are the goals that the relevant industry is trying to achieve. Compared with the application of chemical nematicides, the use of natural plant extracts The plant protection preparation has the following advantages: the natural plant protection preparation has low toxicity, is innocent to human health and does not cause environmental pollution; the plant pathogenic nematode does not produce resistance to the use of the natural plant protection preparation; (non-target organisms) will not cause negative effects; there will be no residual toxicity problems for plant growth; it is cheaper and has no application safety problems compared with nematicides] due to the major inhibitory components of most natural plant extracts. The activity is not stable, resulting in the inability to store for a long time, increasing the difficulty of use, so the plant extract is moderately formulated. The type of preparation that can withstand storage is a subject that must be considered for commercialization. In recent years, there have been two natural nematicides Sincocin and Γ378772 remans Poir., and the sorghum variety plants are equivalent to peat soil and pearl stone. Pots

I 钵' then inoculate the above-mentioned nematode egg mass into the root of the spinach in the potted pot. After about one and a half months, a generation of new egg mass is produced as a source of insects. Southern root rot nematode (Praiy/encMs co/Teae) was first sterilized three times with a mixed disinfectant for fifteen minutes each. The components of the mixed disinfectant include

1000 ppm of streptomycin Sulfate, 1000 ppm of Chloromycetin, and 1 ppm of Penicillin-G. After rinsing three times with sterile water, it was aseptically cultured in the carrot healing group (carr〇t ca丨丨us) for one month and then used. Preparation of inoculation source The southern root nodule nematode used in this experiment was collected from the root nodule of the southern root nodule isolated from the root tissue of the spinach roots before the experiment. After soaking in sterile water for 24 hours, 'incubate with a sterile glass pipette. The first-instar larvae that came out were collected in a small beaker of 1 ml and the amount of insects was calculated. The root rot nematode test source was washed with sterile water to the root rot nematode cultured in the carrot healing tissue, and collected in a small beaker of 彳〇〇ml to calculate the amount of insects for the test. With the test and soil _ supply " type host plant for tomato (/_yCOpers/.c〇A7 esctv/eniL/m) farmers in the dish to the middle of the tomato seeds planted with Basvan Buuren (BVB) " (No.4, Maas丨and, Nether丨ands, the composition is German white peat moss 0 /〇, black mud, 20% moss, sand 1〇〇/.; pH6 32 ; EC〇7〇6dS/m; (0.073%) black plastic seedlings in the tray (12χ24 grid), every 1387772-small (2.5x2.5cm) sowing - to two seeds, the length of the two cotyledons when fully expanded, transplanted to contain steam Disinfect the cultivated soil pots (diameter "5 cm), transplant a seedling in each pot, drink water once a day in the morning and evening, and spray it with a 300~500 times solution of leaf fertilizer (Lion horse) every week to maintain Good growth, planting 30 to 35 days of seedlings as test plants. For the purpose of this experiment: the soil is taken from the land of Pingyuli, Caotun Town, Nantou County, and the soil of the forest is analyzed for sandy bad soil. (sandy 丨 sun), the pH value is 67, the water content is texture with sand (sand) accounting for 60.4%, clay (_) accounting for 25 6%, granule (10)) accounting for 14%. After the vegetable steam sterilization oil main, ^, 〇 Guang ... / Feima _ / Xiao Xiao (121 C, 24 hours) after the spare. A variety of natural plant protection agents for Jie Qi & +, "worm and southern smashing carpet Yiyin solution rate and second-instar larvae have been vigorously put forward in recent years by the Agricultural Experimental Institute. The use of natural plant materials such as large scale, pepper, wood vinegar, etc. to control the occurrence of crop pests and diseases, also developed a The series of natural plant extracts A, κ:, ·, injury plant protection preparations such as Cai '... Road, Jing Tan, Zheng Fen, Hei Xiluo φ, ι罗锈, vitality, stability, 唪The use of snails to control crop diseases and toxins and snails has a very good control effect (10,11,12.22). The present invention uses eight kinds of bran stalks to buckle the ground... The preparation (natural plant protectant, NPP), J: is provided by Zeng potential) 丄.., 'the plant pathology group of the Agricultural Laboratory and the applied animal group: 1. Kwai no dew (NPP-A): emulsified Note: 4 oil preparations from /± ,, diluted 200 times for the control of crop powdery mildew; 2. Carbon (NPP- B): Microemulsion containing citrus durum, 丄幻太m 不格精油, diluted 1 000 times for controlling crop anthracnose and white [S] 10 1378772 3. Mildew (NPP-C): containing citronella oil Microemulsion, diluted 1〇〇〇2〇〇〇 times can be used to control crop gray mold; 4. Black Shura (NPP-D): It is a microemulsion of the invention, which is 1:1 by weight: 1 Cinnamon oil, bitter tea saponin, 95% ethanol, diluted 1 〇〇〇 can be used to control rice rice fever and rose black spot; 5. Rust hiding (NPP-E): natural plant extract microemulsion preparation Diluted 1 〇〇〇 times for controlling crop rust; 6. Vital energy (NPP-F): Chinese herbal compound preparation, diluted 1〇〇〇 times available • Control crop rickets; 7· Stable (NPP-G ): An emulsion containing natural vegetable oil for the control of small insects; 8. ° Mai snail (NPP-Η): a product containing a natural surfactant containing 60% morpholine (M, Oxygen) , used to kill the eggs of the snail. The eight natural plant protection preparations were diluted with sterile water into 1〇1, 1〇2, 1〇5x10, 1〇4 and other dilutions, and 1〇ml was poured into a small Petri dish with an inner diameter of 5 cm, and then _ Southern root nodule and southern root rot, insect print and 1 second instar larvae were placed in small petri dishes, and after standing for 5 days, the nematode (four) rate and the second instar larvae were killed. Rate (-jU read (4)), and the nematode eggs and one larvae were transferred to sterile water for 24 hours to determine h re-incubation and second-instar larvae. There were five replicates for each treatment, and the trial was repeated twice with sterile water as the control. ^Effects of human natural plant protection preparation solution on plant pathogenic line drunkenization rate and second-instar larval lethality test, observe the second-instar larva causing π (S3 1378772 mortality rate is the standard of second-instar larvae body stiffness is death) However, plant diseases. The original nematode often has an itching phenomenon in an unsuitable environment. After a period of adaptation, the activity can be restored. To ensure the accuracy of the experiment, the nematode eggs and the second instar larvae need to be separately moved to the sterilized. After 24 hours in the water, it was determined that the eggs were no longer hatched and the second instar larvae had actually died, and the hatching rate of the nematode eggs and the mortality rate of the second instar larvae were calculated. The plant protection tray for the control of the southern root aphids 30~35 days of farmer's No. 301 tomato plants inoculated 1 〇〇〇• Only the second instar larvae of the southern root nodule nematode, do not over-watering after two days of inoculation to avoid the loss of nematodes' and maintain the humidity to invade the tomato roots After 14 days of inoculation with the root nodule nematode, eight natural plant protection agents with different dilution concentrations were started, and the dilution concentrations were 1〇, 彳〇〇, 5〇〇, 1〇〇. 〇 times, 5000 times and 10000 times, watered once every five times, and applied with chemical pesticides (欧xamy|) (40 kg/ha) as a drug control group' with sterile water as a control Group. After 40 days, record the root nodule index (G丨) index of tomato plants inoculated with nematode, and the number of nematodes (#N/1〇〇g SOj|) contained in 100 grams of soil per spring, tomato plants. Top dry weight (Top DW), root dry weight (root dry wei9ht, R0〇t DW) and plant height (height). The nodule index is differentiated in the following ways: The nodule index is divided into: 0: No disease level 1: 1-15% roots have nodule grade 2: 16-30% roots have nodule grade 3: 31 - 50% roots have nodule [S] 12 t grade: 51-100% roots have nodule There were five replicates for each treatment, and the experiment was repeated twice. The effect of the scorpion sputum preparation on the hatching rate of the southern root worm and the southern root rot nematode and the mortality rate of the second instar larvae The four plant parasitic nematodes that were severely affected by crops in Taiwan were selected. Including the two species of the larvae of the diseased shoots, the leaf nematode and the weighted nematode, and the root nodule nematodes and root rot nematodes in the lower part of the two crops as the object of the test', but the preliminary test (pre-test), the results show that the test Eight natural plant protectant (NPP) used - Black Shura, Kwai No Lu (Npp_A), Jingke No. (NPP-B), mildew (NPP_C) 'NppE', vital energy (NppF), stable (NPP-G), and snail (NPP_H) have no lethal response to leaf nematode and pine wood nematode. Please refer to Table 1 for the test of the southern root rot nematode in the lower part of the crop. The test results can be found at a high concentration of 1〇〇 dilution or more, except for the black scrub and mildew, other extracts against the root rot nematode The effect is not good, and the mortality of the root rot nematodes in the shoots of the shoots and the roots of the damaged crops is poor. The life history of the ectoparasitic root rot nematodes will be in the soil. And the body wandering in the plant, the leaf worm and the pine wood nematode have developed a surface structure with high environmental adaptability (5'6), and the relative endurance to the extract used is also high. The post-field experiment was carried out by the southern root nodule nematode, and the control of leaf nematode, pine wood nematode and root rot nematode was further improved. 13 1378772 Table 1 Effect of natural plant protection preparations on the ovation rate of larvae of the southern root rot nematode - ageing larvae

NPP-A $ larvae

10000X 5000X 1000X 500X 100X __1QX NPP-B 55.61 55.0 54.0 61.4 54.0 58.4 1.21.6 1.41.22.21.8

10000X 5000X 1000X 500X 100X 10X 60.6 58.8 57.2 63.6 58.4 58.6 2.02.6 4.0 3.08.8

NPP-C 20.4

10000X 5000X 1 000X 500X 100X 10X 62.0 61.8 60.0 62.2 54.6 32.6 3.42.2 4.2 29.4 89.2

14 1378772

NPP-D 10000X 61.2 7.0 5000X 66.2 7.6 1000X 61.2 18.2 500X 26.8 16.2 100X 27.2 96.6 10X 35.4 92.4 NPP-E 10000X 58.8 5.4 5000X 59.6 5.6 1000X 61.6 8.8 500X 62.0 6.8 100X 53.4 18.2 10X 52.0 25.6 NPP-F 10000X 57.2 6.4 5000X 58.0 6.2 1000X 54.0 5.0 500X 59.6 7.0 100X 59.2 7.4 10X 60.6 20.6 NPP-G 10000X 63.6 7.6 5000X 65.4 5.4 1000X 57.6 5.6 [s] 15 1378772 500X 65.8 6.8 100X 65.2 6.2 10X 65.8 7.4 NPP-H 10000X 61.3 7.6 5000X 65.2 7.4 1000X 50.2 10.6 500X 53.2 7.6 100X 47.4 37.4 10X 45.2 81.6 Control (water) 67.0 17.0 LSD. Os 3.97 3.95 1Check the arithmetic mean in each block with Fisher's least significant difference (LSD) (p = 0.05) (Means, n = 5) °

Referring to Table 2, the results of testing the natural plant protection preparation on the lethal effect of the root nodule nematode show that the effect of the Kwai no-dew and stable extract on the high concentration of 1〇 dilution is extremely poor, and the mortality rate is lower than that. In the following, the 铄 佤 佤 佤 佤 佤 , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , Unsatisfactory work u ^ Good but - after a hundred times dilution, there is a very high rate =: B grade 'has recorded two kinds of hiding and mildews; after 1〇 dilution, the death rate is extreme c-class' Number and vitality can be two types. m 16 1378772 Table 2: The effect of plant protection preparations on the mortality rate of second-instar larvae. The mortality rate of hatching larvae of root nodule eggs (%) 60.01 1.0 64.0 1.2 56.8 1.4 55.4 2.0 52.4 1.2 52.2 12.4 65.0 1.6 65.2 8.0 69.6 17 65.8 14 33.6 26.4 22.0 94.2 67.0 10 65.8 1 1.6 54.4 11.2 53.2 46.4 41.2 100 16.8 100 Natural botanicals / dilution ratio

NPP-A

10000X

5000X

1000X

500X

100X

10X

NPP-B

10000X

5000X

1000X

500X

100X

10X

NPP-C

10000X

5000X

1000X

500X

100X

10X

NPP-D 17 1378772

10000X 30.0 100 5000X 30.6 98 1000X 27.8 98.4 500X 20.6 98.8 100X 13.6 100 10X 14.4 100 NPP-E 10000X 60.2 10 5000X 65.0 1 1.2 1000X 58.2 14.6 500X 33.0 18.6 100X 35.0 97.4 10X 30.8 100 NPP-F 10000X 62.4 11.2 5000X 66.2 9.8 1000X 55.0 8.2 500X 55.6 16.4 100X 33.6 53.4 10X 37.0 97.6 NPP-G 10000X 66.8 2.0 5000X 65.8 1.2 1000X 67.4 2.0 500X 64.4 1.8 [s] 18 1378772

100Χ 57.8 1.2 10Χ 55.4 2.4 ΝΡΡ-Η 10000Χ 26.4 90.2 5000Χ 22.6 98.5 1000Χ 21.6 94.3 500Χ 15.6 97.6 100Χ 18.2 100 10Χ 15.4 100 Control group (water) 69.6 12.6 LSD 〇0S 5.26 0.78 1 with Fisher's least significant difference value (Fisher's Least significant difference, LSD) (P=〇.〇5) test the arithmetic mean in each block (Means, n = 5) ° 2_The effect of natural plant protection preparations on the control of southern root nodule due to the concentration of plant extracts Too high, it is easy to cause the plant to produce phytotoxicity and death, so the use of a very low concentration of A-grade plant extracts - Risuo Luo and ° Mai snails for greenhouse testing. Look at the table - the results of the test showed that the black snail and the snail were carried out. The greenhouse test was carried out at a concentration of more than 1 〇 4 times, and the roots of the plants were tested: the number of larvae and the number of second instar larvae in the soil mites There is a clear downward trend, so the most potential for the development of crop nematode diseases is controlled by ",, t, and snails." The black snail is used to control fungal diseases and kill the snails: this 4 test also η At the same time, it has the potential to prevent and control plant nematode diseases, especially [S3 19 1378772 is the most serious southern test result of Taiwan crops. It is also known that black sorrow and sylvestris sinensis. It is quite good. The effect of prevention and control _ _ _ rate, after the future commercialization, the second child: farmers rate and 3.% 夂 夂 to reduce the farmers' production costs and the willingness of farmers in the South to engage in non-pesticide prevention and treatment have a considerable positive help. Table 2: Effect of application of black snail (ΝΡΡ-D) and snail (NPP-Η) on control of crop root nodule Φ Natural plant Gl Top DW Root DW2 Height No./100g Preparation/dilution ratio ___(〇)___ (cm)_soil NPP-D 10000X 1.21 4.6 1.2 50.8 14.8 5000X 1.2 5.4 1.0 49.6 12.0 1000X 1.6 5.2 2.2 60.6 12.8 500X 1.4 5.3 1.8 56.6 6.4 100X 1.0 5.6 1.6 55.8 4.4 10X 0.8 5.7 2.2 54.0 4.8 npp-h 10000X 2.0 3.2 1.2 44.0 34.2 5000X 1 .8 4.8 1.2 48.4 22.4 1000X 1.0 4.8 1.0 43.0 7.2 500X 1.2 5.4 1.2 55.8 7.6 100X 0.8 4.8 1.6 55.8 5.2 10X 0.2 5.4 1.8 53.4 4.2 Oxamyl 0.4 5.6 2.2 57.6 5.0 [S3 20 1378772 (40kg/ac.) Control group (water) 2.8 5.3 2.2 61.2 55.2 LS-D Please--〇,7 0.56 3.56 3.8 1Check the arithmetic in each column with Fisher's least significant difference value (Fisher, s | east sjgn•丨fjcant difference, LSD) (p=〇.05) Mean (Means, n = 5) 〇 It is also known from the above experimental results that water is hydrolyzed in the soil and the aqueous solution of different dilutions of the snails is used in the root nodule index and the number of second instar larvae in each hundred grams of soil. The results were not significantly different from the administration of the nematicidal agent (Oxamyl) in the drug control group. This result proves that the use of the natural plant protection preparation can also achieve the similar control effect using nematicides. Compared with the application of nematode, the use of natural plant protection preparations has the following advantages: the natural plant protection preparation has low toxicity, is innocent to human health and does not cause environmental pollution; the use of plant pathogenic nematodes for natural plant protection preparations will not It develops resistance; it does not adversely affect non-target organisms; it does not have residual problems for plant growth; it is cheaper and has no application safety issues compared to general chemical nematicides. Therefore, the inventor of the present invention succeeded in using the black mulberry which was originally used for controlling rice rice fever and black spot disease to eliminate root nodule nematodes and root rot nematodes, and even if it is 10,000 times diluted, it has the effect of nematicidal, so the present invention can be sure Unexpected results. I S3 21 1378772 proved to contain cinnamon oil in the above Example 1

..., Shura has the effect of chrysanthemum and green A. Therefore, the natural cinnamon oil and the medicinal alcohol which are purchased from the market are mixed in various proportions, and after being stirred, they become Fugu's, 4- and transparent transparent microemulsions. The effects of microemulsions mixed in various ratios for '10,000> σ root nodule nematodes were tested. Control of nodule nematode effect See Table 4, which shows the mixing ratio of natural cinnamon oil, aryl saponin and ethanol of this example and the nematicidal effect obtained therefrom. Wood" Table 4: Effect of the cinnamon oil microemulsion (diluted 10, 〇〇〇 times) of the present invention on the rate of nematode infection and the mortality of second-instar larvae

30 33 30 47.5 100 2 2 3 :2 : 1.5 6 6 36.8 98 100 95 98 Example 3. According to the 3rd of May, 1989, the Agricultural Commission of the Executive Yuan, 89 Agricultural Grains No. 890020475/^ The (ACCe|erated storage procedure) method was used to test the stability of the microemulsion. The microemulsion of the present invention (natural meat plug oil is placed at 54±2° C., the mixture ratio of bitter tea saponin and ethanol is 1:1:1), and the mixture is allowed to stand for 14 days, and then taken out and allowed to stand. In the chamber IS] 22, the dish was turned to the temperature, and no micro-emulsion was delaminated or discolored. This high temperature test shows that the microporous agent can be stored at room temperature for three years without being denatured, so that it has stability. In this case, the micro-emulsification technology is used to combine vegetable oil with water and ethanol to help 〇/vv #微乳胄. Through a series of tests, the microemulsion can be used to control diseases caused by crop root nodule nematodes. Moreover, the microemulsion of the present invention has a relatively high stability and can be stored for more than three years without being denatured under normal environmental conditions, and has a long shelf life. • [Simple description of the schema] Benefits [Key component symbol description] Benefits [References] 1. Wang Jintang, 2004, Application of Repellent Crops, Proceedings of Life Safety Strategy Symposium, pp. 77-98. 2. Yu Zhiru, 2004, Current situation of research on pest control of plant extracts, Proceedings of the Symposium on Quality and Life Strategies for All-Agricultural Farmers, ρρ.ι 29-1 39. 3. Hu Minfu, 2004, Introduction to natural plants against pathogens and viruses and research and development of plant protection preparations, seminar on quality and safety agricultural products life strategy, PP.99-1 16. 4. Cai Dongyu, 1 999, Chemical Control of Plant Parasitic Nematode Diseases, Plant Pathology Journal 8:41-50. 5_Cai Dongyu, 1998, Evolution of Plant Parasitic Nematodes, Plant Pathology Journal 7: 1-9. 6. Cai Dongyu, 2003, Plant parasitic nematode diversity, microorganisms

I SJ 23 1378772 ··· The Collection of Symposiums on Diversity and Sustainable Use, ρρ·78_112. Song 7·Cai Dongyu, 2004, Proceedings of the Biological Control of Plant Pathogenic Nematodes, Symposium on the Life Strategies of Quality and Safe Agricultural Products. ρρ.59-76. 8_Xie Baoquan, 2000, Antibacterial effect of cinnamon extract, Taiwan Agricultural Chemistry and Food Science 38(2): 184-193. 9-Xie Tingfang, 2004, Research on the control of crop diseases by plant extracts, Proceedings of the seminar on the life strategy of the whole country, ρρ117_128. 1〇·谢廷芳' Yan Zhengchang, Xie Lijuan, Li Guizhi, Hu Minfu, 2〇〇4, The effect of Chinese herbal medicine extract on the control of Chinese cabbage anthracnose, Plant Diseases Journal] 3: 345 (abstract). 11·Xie Tingfang, Hu Minfu, Huang Jinxing, Ke Wenxiong, 2005, Natural Plant Protection Preparation Kwai Bulu, a practical plant protection material, Fengnian 55(8): 63-64. 12. Xie Tingfang, Hu Minfu, Huang Jinxing, Ke Wenxiong, 2〇〇5, suitable for organic planting natural plant protection preparations - Vital Energy Year 55 (12): 44-45 〇

13. Bharathi, Μ. 1999. Effect of plant extract and chemical inhibitors on cucumber mosaic virus of brinjal. J. of Mycology and Plant Pathology 29(1): 57-60. 14. Bilgrami, AL 1997. Nematode biopesticides. Aligarh Muslim University, India. 228 pp. 15. Daayf, F., Schmitt, A., and Belanger, RR 1995. The effects of plant extracts of Reynoutria sachalinensis on powdery mildew development and leaf physiology of long English cucumber. Plant disease 79: 577 -580. 16. Foughtk, L., and Kuc, JA 1996. lack of 1 S] 24 1378772 specificity in plant extracts and chemicals as inducers of systemic resistance in cucumber plants to anthracnose. J. Phytopathol. 144: 1-6. 17. Huang, JW; 1992; Integrated management of vegetable seedling pests with a formulated plant nutrition; Plant Prot. Bull. 34:54-63. 18. Inderjit and Mukerji, KG; 2006;

Allelochemicals: biological control of plant pathogens and diseases; Springer, Netherlands. 214 pp. 19. Koul, 0., and Dhaliwal, G. S.; 2001;

Phytochemical biopesticides. Harwood academic publishers; Australia. 223 pp. 20. Regnault-Roger, C., Philogene, B., and Vincent, C.; 2005; Biopesticides of plant origin; Intercept Ltd, UK 313 pp. 21. Tsay, TT, Wu, S. T_, and Lin, YY; 2004; Evaluation of Asteraceae Plants for Control of

Meloidogyne incognita. Journal of Nematology 36(1): 36-41. 22. Wu, DC, Yu, JZ, Chen, BH, Lin, CY, and Ko, WH; 2004; Inhibition of egg hatching with apple wax solvent as a Novel method for controlling golden apple snail {Pomacea canaliculata)·, Crop Protection 24: 483-486. 23. Gasco, MR; 1997; Microemulsions in the I S3 25 1378772 pharmaceutical field: perspectives and applications; Pages 97-122 in: Industrial Applications of

Microemulsions. Marcel Dekker Inc., New York.

IS] 26 1378772 I-1—

j April, 2011 correction of replacement page ~I

DiTeraTM is commercialized. Sincocin is four plants (Ongman's Pruning, Opuntia engelmannii Salm-Dyck ex Engelm., prickly pear cactus; red oak, Qi; erci7s fa/cafe Michex., southern red oak; fragrant salt wood 'rms aromai/ca Ait., fragrant sumac; common mangrove, f?A)/zop/?ora mang/e L., red mangrove) extracts mixed aqueous solution; and DiTeraTM is nematode parasitic fungus Myr〇f/7ec/um vemvcar/ a D. R_ Whitaker The product of bacterial fermentation ABG-9008. Both have good effects in controlling plant nematode diseases. However, the results of Taiwan's high climate/the most recent house's test showed that Sjncocin and DiTe "aTM had a significant effect on controlling the root nodule nematode under the dilution factor of 1 〇〇, and there was no control effect under high dilution. [Invention] In view of the fact that the inventors did not currently have a control for the control of the root nodule nematode under high dilution, it is not economical. Therefore, after long-term research and continuous testing, the microemulsion for controlling the root nodule nematode has finally been invented. The invention provides a microemulsion for controlling the root nodule of crops, which comprises: cinnamon oil, the weight of which accounts for 20%~35 % of the weight of the whole microemulsion; ★ wood saponin, weight 1 system occupies the weight of the whole microemulsion 30%~60%; ethanol's heavy sputum accounts for 2〇%~5〇% of the total microemulsion weight. Preferably, the above meat 祛, 占, 戈父 4 U 桂, from saponin and The weight ratio of ethanol is 1 : 1 : 1 〇 wherein the ritual agent is an oil-in-water type (O/W type). According to the present invention, the crop nodule nematode refers to any one that would cause a 1378772 _ί_ 101 year 4 Monthly S-day correction replacement The root nodule of the plant or crop, and the nodule nematode that causes the victim to form a tumor mainly occurs in the "previously, the crop root nodule nematode, the southern root nodule nematode {Meloidogyne incognita". The present invention further provides a control of the crop root nodule nematode A plant protection preparation comprising at least the above microemulsion β. The invention further provides a spreading agent comprising at least the above microemulsion. The invention further provides a nematicide comprising at least the microemulsion described above, wherein the nematicide The invention further provides a pesticide product comprising the above microemulsion and a carrier, a excipient, a diluent or a solvent. The invention further provides a pesticide for controlling a root nodule nematode, It comprises a granule and the above microemulsion formulated in the solvent. Preferably, the solvent is in a ratio of from 1:0.01 to 1:10000 with the microemulsion. Preferably, the solvent The invention further provides a use of the above microemulsion for the manufacture of a pesticide for controlling a crop of Rhizoctonia solani. The invention further provides a The method for controlling a root nodule nematode comprises: providing a microemulsion as described above; and applying the oil-in-water microemulsion to a plant to avoid the plant suffering from a root nodule nematode disease. wherein the crop root nodule is a southern nodule Nematode. Among them, the plant is tomato, spinach, groundnut, leeks, celery, onion, courgette, melon, watermelon, carrot, soybean, mung bean, guava, etc. It is preferred that the nematicide is used to kill Southern root nodule nematode. -$__ April 2011 S-day modified replacement page The present invention further provides a method for preparing a microemulsion for controlling a root nodule nematode, which comprises 20% to 35% by weight of the total microemulsion of cinnamon/ It is composed of a mixture of 30% to 60% by weight of the total microemulsion, and a mixture of 95% (v/v) ethanol, which is 20% to 50% by weight of the total microemulsion. Wherein the weight ratio of the above-mentioned cinnamon oil, bitter tea saponin and ethanol is 1:1 : 1 〇 wherein the preparation method further comprises diluting the mixed microemulsion to within 10,000 times in a solvent. Preferably, the mixed microemulsion is water-added to dilute the microemulsion to within ten thousand times. Wherein the crop root nodule is a southern root nodule nematode. The effect of the invention is that after the cinnamon oil forms a microemulsion, it can prolong the storage time, and can be used as a plant protection preparation, a nematicide agent, a spreading agent, etc. for agriculture, and has the effect of controlling or eliminating the nematode. The microemulsion of the invention comprises cinnabar oil mixed with cinnamon oil, bitter tea saponin and ethanol, and the microemulsion can effectively reduce the incidence of the southern root nodule nematode within 10,000 times dilution, which can only be compared with the general plant extract. Diluted 2 times in use, has a high degree of progress, and the control effect is equivalent to a generally effective chemical, but unlike general chemical agents, the microemulsion of the present invention has high stability and is directly diluted with water when used. It can be used, so it will not pollute the environment or soil, and the price is lower than that of general chemical pesticides, which is very competitive in the market. [Embodiment] The term "microemulsion" as used herein refers to a technique for preparing a microemulsion by an emulsion composed of an oil-water-interface activity 1378772, a replacement of a page-assisted surfactant, and the like. It is called Microemulsion technology (23). The theory and applied research of microemulsification technology has developed rapidly since 1980. Especially in the late 1990s, the application research of microemulsion technology developed faster' in tertiary oil recovery, wastewater treatment, extraction separation, catalysis, Food, medicine, cosmetics, nano-material preparation, chemical reaction media, coatings industry and other fields have an infinite application prospect. The invention provides a microemulsion for controlling the root nodule of crops, which comprises 20%~35% of the weight of the whole microemulsion, and the weight is 30%~60% of the weight of the whole microbial agent. The phytochemical, and the weight of the whole microemulsion, is 20% to 50% of ethanol, and its concentration is 95 〇 / 0 (v / v). Moreover, the microemulsion can be used as a plant protection preparation, a nematicide, a spreading agent, etc., for controlling or eliminating the southern root nodule nematode. EXAMPLES The following detailed examples provide a more specific manufacturing method to enable those of ordinary skill in the art to understand the contents of the present case, but are not intended to limit the cost of the case (there is therefore only to follow the principles and concepts of the present case). A few changes still belong to the mouth of the present invention. Example 1. The plant parasitic nematode source used in this embodiment is provided by the National ZTE Plant of the Plant Pathology Laboratory of the Department of Plant Pathology. It is mainly capable of cultivating nematodes in Dali. The culture of A. elegans (10) / wogn () is first planted in the greenhouse (/p〇/?7〇ea)

Claims (1)

1378772 r--ς_, VII, the scope of application for patents: L101 April, the end of the replacement page 1. A micro-patch for controlling the root nodule of nematodes, including: cinnamon / /, lining accounted for the overall microemulsion 20%~35% by weight; bitter tea saponin, :, heavy weight accounts for 3〇%~6〇% of the weight of the whole microemulsion; and ethanol has 20%~50% of the weight of the whole microemulsion. 2. The microemulsion for controlling a root nodule nematode according to the scope of the patent application, wherein the weight ratio of the above cinnamon oil, bitter tea, and ethanol is 1:1:1. Φ 3 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 4. The microemulsion for controlling a root nodule nematode according to item 2 of the patent application wherein the emulsifier is of the oil-in-water type. 5. The microemulsion for controlling a root nodule nematode according to any one of claims 1 to 4, wherein the crop root nodule is a southern root nodule line A {Meloidogyne incognita) 〇 6. a species controlling a root nodule nematode A plant protection preparation comprising at least the microemulsion of any one of claims 1 to 5. A microemulsion according to any one of claims 1 to 5, which comprises at least one of the inventions. 8. A nematicide agent comprising at least one of the microemulsions of any one of claims 1 to 5, wherein the nematicide is for killing a southern nematode. A pesticide according to any one of claims 1 to 5, which comprises a carrier, an excipient, a diluent or a solvent. 27 I ... Ding 1 . A medicinal product for controlling the root nodule of crops, which comprises a solution and a solution in the solvent such as Shen/A. A microemulsion as described in any one of claims 1 to 5 of the patent.叮 1 1. A pesticide product of the patent scope of the worm, wherein the solvent is between 1:10,000. 1 The above-mentioned micro-emulsion system for controlling crop root nodule is in a ratio of 1:0 01. 12. The pesticide produced in the control of the root nodule nematode of the crop according to the middle item (4) is the water. . A method for controlling a root nodule nematode of the crop, comprising: providing the above microemulsion; and applying the oil-in-water microemulsion to a plant to avoid the plant suffering from a crop root nodule nematode disease. 14. The method of claim 13, wherein the crop root nodule is a southern root nodule nematode. 15. The method of claim 13 or claim 14, wherein the plant is tomato, spinach, groundnut, amaranth, celery, onion, courgette, melon, watermelon, carrot, soybean, mung bean or guava. 16. The method of claim 13 or 14, wherein the microbial agent is administered once a week for five consecutive weeks. 1 plant is a microemulsion for controlling the root nodule of crops, which is composed of the following: cinnamon oil, the weight of which is 2% to 35% of the weight of the whole microemulsion; the weight of the ancient pigment is the whole microemulsion. 3〇%~6〇% by weight; and 95% (v/v) ethanol, the weight of which is 2〇%~5〇% of the weight of the whole microemulsion. A method for preparing a microemulsion for controlling a root nodule of a crop, comprising a cinnamon oil having a weight of 2% to 35% by weight based on the total microemulsion, and a weight of 30% to 60% by weight of the total microemulsion. Tea saponin and weight account for the overall microemulsion 28 1 4 1 4 曰 替换 correction replacement page J weight 2 〇〇 /. Mixing ~50% ethanol. 19. As stated in the scope of claim 18, the oil, the signature and the main ingredient, the weight ratio of the cinnamon ancient tea saponin and ethyl yeast is 1: 1: 20. The method according to claim 19, wherein the preparation further comprises diluting the microemulsion after the '/3⁄4 dilution by one solvent. 21_ as described in claim 19 ^ Method, wherein the mixed microemulsion is added with water to dilute the microemulsion to -, ° 禹. 22. As described in the scope of claim 19, 20 or 21, the method described in the paragraph, +, Wherein the crop root nodule is a southern root nodule nematode. 8. Pattern: None 29