1360573 100年.12月20日核正巷_百 六、發明說明： 【發明所屬之技術領威】 [0001]本發明涉及細胞培養領域，尤其涉及一種細胞定向培養 系統、細胞定向培養基板及培養定向細胞的方法。 【先前技術】 [0002] 神經系統主要由神經元（neurons )以及神經膠質細胞 (neuron glial cells)構成一複雜且特異的溝通網域 ，用以與其他組織或器官建立連結以進行功能協調。神 經系統中，接收刺激’通過傳導並輸出神經傳導物質 (neuron transmitter)以進行組織或器官間訊息溝通 是由神經元來執行’而神經膠質細胞則執行神經元物理 性支持，營養提供以及調節溝通訊息速度等功能。 [0003] 每一神經元依據型態包含胞體（cell body)與神經突起 (neurite)兩部分’神經突起自胞體延伸並朝向其他神 經元或是其他細胞（例如：肌肉細胞）生長，其中神經突 起又分為轴突（axon)與樹突（dendrite)兩種。一般來 說，刺激由樹突接收並將衝動傳向胞體，衝動經過軸突 傳導至轴突末端’並釋放傳導物質來觸動其他細胞。 [0004] 由於神經系統扮演生物體内各組織與器官之間的協調作 用，其之重要性不言可喻》—旦神經元受到損害，甚而 更嚴重時，神經突起發生斷裂而收縮，受損部分兩端會 因而產生一間隙°在最佳狀態下，此間隙可藉由神經元 的再生修復能力’讓神經突起重新生長而再次建立起神 經元的訊號傳遞能力，然而，在大部分情況下’除非神 經元的損害非常微小，且由於重新生長的神經突起係往 097130294 表單編號A0101 $ 4頁/共19頁 1003473157-0 13605731360573 100 years. December 20th, nuclear positive lane _ hundred six, invention description: [Technology of the invention] [0001] The present invention relates to the field of cell culture, in particular to a cell-oriented culture system, cell-oriented medium plate and culture A method of directional cells. [Prior Art] [0002] The nervous system is mainly composed of neurons (neurons) and neuron glial cells, which constitute a complex and specific communication domain for establishing links with other tissues or organs for functional coordination. In the nervous system, receiving stimuli 'transmitting and outputting neuron transmitters for tissue or organ communication is performed by neurons' while glial cells perform neuronal physical support, nutrient supply, and regulatory communication. Features such as message speed. [0003] Each neuron depends on the type comprising a cell body and a neurite, the neurites extending from the cell body and growing toward other neurons or other cells (eg, muscle cells), wherein The neurites are divided into axons and dendrites. In general, the stimulus is received by the dendrites and transmits impulses to the cell body, impulsively transmitted through the axons to the axon tip' and releases the conductive material to trigger other cells. [0004] Since the nervous system plays a coordinating role between tissues and organs in the living body, its importance is self-evident." Once the neurons are damaged, even more severe, the neurites are broken and contracted, and damaged. Some of the ends will create a gap. In the optimal state, this gap can re-grow the neurites by the neuron's regenerative ability to re-establish the signal transmission capability of the neurons. However, in most cases, 'Unless the damage to the neurons is very small, and due to the regrowth of the neurites towards 097130294 Form No. A0101 $ 4 pages / Total 19 pages 1003473157-0 1360573
100年i2月日修正替換頁 多方向生長，因此通常重新生長的神經突起仍無法連接 起間隙的兩端。 [0005] 有鑑於此，如何提供一種能夠導引細胞定向生長的細胞 定向培養系統、細胞定向培養基板及培養定向細胞的方 法，實為現今的重要課題之一。 【發明内容】 [0006] 提供一種細胞定向培養系統，其係包括一基底、一培養 層以及一培養液。其中，培養層包括至少一奈米碳管設 置於基底上，奈米碳管膜包括至少一奈米碳管繩，各奈 米碳管繩係包括複數個奈米碳管束片段，其中相鄰奈米 碳管束片段之端部依次相互連接，且奈米碳管束片段包 括複數個平行排列之奈米碳管；培養夜係覆蓋培養層， 培養液包括至少一細胞成長因子促進一細胞沿奈米碳管 生長。 [0007] 另外，亦提供一種細胞定向培養基板，其包括一基底以 及一培養層。其中，培養層係包括至少一奈米碳管膜設 > 置於基底上，奈米碳管膜包括至少一奈米碳管繩，各奈 米碳管繩包括複數個奈米碳管束片段，其中相鄰奈米碳 管束片段之端部係依次相互連接，且奈米碳管束片段係 包括複數個平行排列之奈米碳管。 [0008] 再者，亦提供一種培養定向細胞的方法，其包括下列步 驟：提供一基底；提供至少一奈米碳管膜，其係藉由拉 伸一奈米碳管陣列所形成，其中奈米碳管膜包括至少一 奈米碳管繩，奈米碳管繩包括複數個奈米碳管束片段， 相鄰奈米碳管束片段之端部依次相互連接，且奈米碳管 097130294 表單編號 Α0101 第 5 頁/共 19 頁 1003473157-0 1360573 100年.12月20日梭正替換頁 束片段包括複數個平行排列之奈米锼管；設置奈米碳管 膜於基底上，提供一培養液覆蓋奈米碳管，培養液包括 至少一細胞成長因子；以及種植一細胞於培養液中，細 胞係沿奈米碳管生長。 [0009]The 100-year-old i2 month correction replacement page grows in multiple directions, so the neurites that are usually re-growth still cannot connect the ends of the gap. [0005] In view of this, how to provide a cell-directed culture system, a cell-directed culture medium plate, and a cultured directional cell capable of guiding cell-oriented growth is one of the important issues today. SUMMARY OF THE INVENTION [0006] A cell-directed culture system is provided which includes a substrate, a culture layer, and a culture solution. Wherein, the culture layer comprises at least one carbon nanotube disposed on the substrate, the carbon nanotube membrane comprises at least one carbon nanotube string, and each of the carbon nanotube strands comprises a plurality of carbon nanotube bundle segments, wherein adjacent nanotubes The ends of the carbon nanotube bundle segments are connected to each other in turn, and the carbon nanotube bundle segments comprise a plurality of parallel arranged carbon nanotubes; the cultured night-covering culture layer comprises at least one cell growth factor promoting one cell along the nanocarbon Tube growth. Further, a cell-oriented medium plate comprising a substrate and a culture layer is also provided. Wherein, the culture layer comprises at least one carbon nanotube membrane and is disposed on the substrate, the carbon nanotube membrane comprises at least one carbon nanotube rope, and each of the carbon nanotube ropes comprises a plurality of carbon nanotube bundle segments. The end portions of adjacent carbon nanotube bundle segments are connected to each other in turn, and the carbon nanotube bundle segments comprise a plurality of carbon nanotubes arranged in parallel. [0008] Furthermore, a method for culturing a directional cell is also provided, comprising the steps of: providing a substrate; providing at least one carbon nanotube film formed by stretching an array of carbon nanotubes, wherein The carbon nanotube film comprises at least one carbon nanotube rope, and the carbon nanotube rope comprises a plurality of carbon nanotube bundle segments, the ends of adjacent carbon nanotube bundle segments are connected to each other in turn, and the carbon nanotubes 097130294 form number Α0101 Page 5 of 19 1003473157-0 1360573 100 years. On December 20th, the shuttle replacement segment contains a plurality of parallel aligned nanotubes; a carbon nanotube film is placed on the substrate to provide a culture solution coverage. The carbon nanotubes, the culture solution includes at least one cell growth factor; and the planting of a cell in the culture medium, the cell line growing along the carbon nanotube. [0009]
承上所述’因依細胞定向培養系統、細胞定向培養基板 及培養定向細胞的方法，係铺設至少一奈米碳管膜於基 底上用以種植細胞，藉由奈米碳·管膜中平行排列的奈米 碳管，種植於其上的細胞係可沿奈米碳管生長而達到定 向，相較於習知技術，應用於受損細胞的再生長，可導 引細胞依尋一特定方向生長’進而達到細胞修復的作用 【實施方式】 [0010] [0011] 下面將結合附圖對實施方式作進一步之詳細描述<» 請參照圖1與圖2所示，依據較佳實施例之一種細胞定向 培養系統1係包括一基底10、一培養層12以及一培養液14 。培養層12係設置於基底10上，培養液14係覆蓋培養層 12。在本實施例中，細胞定向培養系統1係用以培養一神 經細胞2使其定向生長。其中，神經細胞2係為一海馬神 經細胞（hippocampal neuron) 〇 [0012] 在本實施例中，基底之材質係為高分子材料，且基底 1〇係形成一框架結構或是為一蓋玻片。 培養層12係包括至少一奈米碳管膜122 ’鋪設於基底10上 。如圖3所示，奈米碳管膜122係包括至少一奈米碳管繩 124，奈米碳管繩124係由複數個奈米碳管束片段126藉 097130294 表單編號A0101 第6頁/拜19頁 1003473157-0 [0013] loo年:iz月zo目修正_頁 由凡德瓦爾力（Van der Waals)依次連接其之端部而形 成，其中每個奈米碳管束片段126係具有大致相等的長度 ，且奈米碳管束片段126由複數個相互平行排列的奈米碳 管128所構成。 培養層12除了以單層之奈米碳管膜122構成外’培養層12 亦可包括複數個奈米碳管膜122 ’在本實施例中’培養層 12係包括3至5層之奈米碳管膜122鋪設於基底10上’且 該等奈米碳管膜122係交互疊置於基底10之至少一表面上 ，形成厚度範圍為10. Onm至200. Onm之間的培養層12。 於多層奈米碳管膜122所構成的培養層12中’各層奈米碳 管膜1 22之間的奈米碳管128的排列方向係呈相互平行， 即依據奈米碳管128的排列方向為相互平行的方式疊置多 層的奈米碳管膜122。 在本實施例中，奈米碳管128係為一多壁奈米碳管 (mutiwalled carbon nanotubes)，且該等奈米破管 128的平均直徑範圍係約為10. Onm至20_ Onm，然而，構 成奈米碳管膜122的奈米碳管128，並不僅限於多壁奈米 碳管的應用，另外’單壁奈米碳管（single-walled carbon nanotubes)構成的奈米碳管膜122亦可用以作 為細胞2成長的培養層12。在本實施例中，該等奈米碳管 128的平均直徑係是以15. Onm為例說明。另外，而為了 增加細胞定向培養系統1的親水性與生物相容性 (biocompatibility) ’奈米碳管128可藉由物理方法以 及化學方法進行功能性修飾’舉例來說，奈米碳管128係 以物理吸附方式處理使其具有宓（pyrene)或蒽 表單編號A01.01 第7頁/共19頁 1003473157-0 1360573 100年.12月20日梭正替换頁 (anthracene)等的修飾，又，奈米碳管128可以化學方 式使其具有羧基的修飾。 [0016] 培養液14係包括至少一細胞成長因子（cell growth factor)，其係包覆至少部份之奈米碳管128，用以促進 種植之細胞2沿該等奈米碳管128生長，而依據細胞2的種 類不同，選用的細胞成長因子亦可不同，在本實施例中According to the above-mentioned method for directional culture of cells, cell-directed medium plates and culture of directional cells, at least one nanotube membrane is laid on the substrate for planting cells, which are parallel in the nanocarbon membrane. The aligned carbon nanotubes, the cell lines planted thereon can be oriented along the carbon nanotubes to achieve orientation, which is applied to the regrowth of damaged cells compared to the prior art, and can guide the cells to find a specific direction. [Embodiment] and the effect of cell repair [Embodiment] [0011] The embodiment will be further described in detail below with reference to the accompanying drawings. Referring to FIG. 1 and FIG. 2, according to a preferred embodiment, A cell-directed culture system 1 includes a substrate 10, a culture layer 12, and a culture solution 14. The culture layer 12 is disposed on the substrate 10, and the culture solution 14 covers the culture layer 12. In the present embodiment, the cell-directed culture system 1 is used to culture a neuron cell 2 for directional growth. Wherein, the nerve cell 2 is a hippocampal neuron 00 [0012] In this embodiment, the material of the substrate is a polymer material, and the substrate 1 is formed into a frame structure or a cover glass. . The culture layer 12 includes at least one carbon nanotube film 122' laid on the substrate 10. As shown in FIG. 3, the carbon nanotube film 122 includes at least one carbon nanotube string 124, and the carbon nanotube string 124 is borrowed from a plurality of carbon nanotube bundle segments 126. 097130294 Form No. A0101 Page 6/Bai 19 Page 1003473157-0 [0013] loo year: iz month zo target correction _ page is formed by Van der Waals in turn connecting its ends, wherein each carbon nanotube bundle segment 126 has approximately the same The length, and the carbon nanotube bundle segment 126 is composed of a plurality of carbon nanotubes 128 arranged in parallel with each other. The culture layer 12 is composed of a single layer of carbon nanotube film 122. The 'culture layer 12 may also include a plurality of carbon nanotube films 122'. In this embodiment, the culture layer 12 includes 3 to 5 layers of nanometer. The carbon nanotube film 122 is laid on the substrate 10' and the carbon nanotube film 122 is alternately stacked on at least one surface of the substrate 10 to form a culture layer 12 having a thickness ranging from 10. Onm to 200. Onm. In the culture layer 12 composed of the multilayered carbon nanotube film 122, the arrangement direction of the carbon nanotubes 128 between the layers of the carbon nanotube film 1 22 is parallel to each other, that is, according to the arrangement direction of the carbon nanotubes 128. The multilayered carbon nanotube film 122 is stacked in a mutually parallel manner. In the present embodiment, the carbon nanotubes 128 are a plurality of mutiwalled carbon nanotubes, and the average diameter of the nanotubes 128 is about 10. Onm to 20_Onm, however, The carbon nanotubes 128 constituting the carbon nanotube film 122 are not limited to the application of the multi-walled carbon nanotubes, and the carbon nanotube film 122 composed of 'single-walled carbon nanotubes' It can be used as the culture layer 12 in which the cells 2 grow. In this embodiment, the average diameter of the carbon nanotubes 128 is exemplified by 15. Onm. In addition, in order to increase the hydrophilicity and biocompatibility of the cell-directed culture system 1 'nano carbon tube 128 can be functionally modified by physical methods and chemical methods', for example, a carbon nanotube 128 series It is treated by physical adsorption to have a pyrene or 蒽 form number A01.01 page 7 / 19 pages 1003473157-0 1360573 100 years. December 20th, the replacement of the page (anthracene), etc. The carbon nanotubes 128 can be chemically modified to have a carboxyl group. [0016] The culture fluid 14 includes at least one cell growth factor that coats at least a portion of the carbon nanotubes 128 to promote growth of the planted cells 2 along the carbon nanotubes 128, Depending on the type of the cell 2, the selected cell growth factor may be different, in this embodiment.
，細胞成長因子係為一神經成長因子（nerve growth factor, NGF)或一驗性成纖維細胞成長因子（basic fibroblast growth factor, bFGF)° [0017] 本實施例之細胞定向培養系統1更包括一電源（圖未顯示） ，用以在培養過程中提供一電流訊號刺激神經細胞2，使 其定向生長出突起，並沿奈米碳管128伸展。 [0018] 另外，請參照圖3與圖4所示，依據較佳實施例之一種培 養定向細胞的方法，其係包括下列步驟： [0019] 步驟S1，提供一基底10，該基底10之材質係可為高分子 材質作為一支撐基板。 [0020] 步驟S2，提供至少一奈米碳管膜122，該奈米碳管膜122 係藉由拉伸一奈米碳管陣列11所形成（如圖3所示）。在本 實施例中，奈米碳管膜122的製造方法係包括下列步驟： [0021] 首先，提供一基板，例如矽基板；接著，利用電子束蒸 發法、熱沈積或濺射等方法將一催化劑沉積於基板表面 上；再通入包含碳源氣與保護氣體之混合氣體使奈米碳 管陣列11從基板上長出；從奈米碳管陣列11中選定一包 括複數個奈米碳管128之奈米碳管束片段126，利用一拉 097130294 表單編號A0101 第8頁/共19頁 1003473157-0 1360573 100年12月20日修正替換頁 伸工具拉伸該奈米碳管束片段126，而使奈米碳管繩124 沿拉伸方向形成，進而形成該奈米碳管膜122。 [0022] 在本實施例中，所形成的奈米碳管膜122係包括至少一奈 米碳管繩124，且奈米碳管繩124係包含複數個平行排列 的奈米碳管128。 [0023] 步驟S3，設置奈米複管膜122於基底1 0上，在本實施例中 ，奈米碳管膜122係鋪設於基底10之至少一表面上，另外 ，基底10上亦可利用疊置複數個奈米碳管膜122來形成一 φ 培養層12，且各奈米碳管膜122係使奈米碳管128的排列 方式呈相互平行來疊置。鋪設好奈米碳管膜122之基底10 係用以放置於一培養皿3中。 [0024] 步驟S4，提供一培養液14覆蓋該等奈米碳管128，培養液The cell growth factor system is a nerve growth factor (NGF) or a basic fibroblast growth factor (bFGF). [0017] The cell-oriented culture system 1 of the present embodiment further includes a A power source (not shown) is provided to provide a current signal during the culturing process to stimulate the nerve cells 2 to grow toward the protrusions and to extend along the carbon nanotubes 128. [0018] In addition, referring to FIG. 3 and FIG. 4, a method for cultivating directional cells according to a preferred embodiment includes the following steps: [0019] Step S1, providing a substrate 10, the material of the substrate 10 The polymer material can be used as a supporting substrate. [0020] Step S2, at least one carbon nanotube film 122 is provided, which is formed by stretching a carbon nanotube array 11 (as shown in FIG. 3). In the present embodiment, the method for manufacturing the carbon nanotube film 122 includes the following steps: [0021] First, a substrate, such as a germanium substrate, is provided; then, a method such as electron beam evaporation, thermal deposition, or sputtering is used. The catalyst is deposited on the surface of the substrate; the carbon nanotube array 11 is grown from the substrate by introducing a mixed gas containing the carbon source gas and the shielding gas; and the carbon nanotube array 11 is selected from the plurality of carbon nanotubes 128 nm carbon tube bundle segment 126, using a pull 097130294 Form No. A0101 Page 8 / Total 19 pages 1003473157-0 1360573 December 20, 100 revised replacement page stretching tool to stretch the carbon nanotube bundle segment 126, so that The carbon nanotube string 124 is formed in the stretching direction to form the carbon nanotube film 122. [0022] In the present embodiment, the formed carbon nanotube film 122 includes at least one carbon nanotube string 124, and the carbon nanotube string 124 includes a plurality of parallel arranged carbon nanotubes 128. [0023] Step S3, the nano tube film 122 is disposed on the substrate 10, and in the embodiment, the carbon nanotube film 122 is laid on at least one surface of the substrate 10, and the substrate 10 can also be utilized. A plurality of carbon nanotube films 122 are stacked to form a φ culture layer 12, and each of the carbon nanotube films 122 is arranged such that the arrangement of the carbon nanotubes 128 is parallel to each other. The substrate 10 on which the carbon nanotube film 122 is laid is placed in a petri dish 3. [0024] Step S4, providing a culture solution 14 covering the carbon nanotubes 128, the culture solution
14係包括至少一細胞成長因子。在本實施例中’培養液 14的製備係可將細胞成長因子（例如：神經成長因子或鹼 性成纖維細胞成長因子）溶於一生物媒質（biological m e d i a)中，而生物媒質則可依據培養細胞種類的不同而 調製。 [0025] 步驟S5，種植一細胞2於培養液14中，種植之細胞係貼附 於奈米碳管膜122上。於此，細胞2在生物媒質的滋養以 及細胞成長因子的促進下，細胞2係沿平行排列的奈米碳 管128定向生長。 [0026] 在本實施例之培養定向細胞的方法，更可包括一步驟： 提供一電流訊號刺激細胞2，意即，在培養的過程中藉由 一電源輸出微小的電流訊號來刺激細胞2 (例如神經細胞） 097130294 表單编號 A0101 第 9 頁/共 19 頁 1003473157-0 1360573 [0027] [0028] [0029] [0030] [0031] [0032] 100年.12月20日修正替七百 ’來加速細胞2的生長速度。 另外’在步驟S3之後，更包括一步驟：修飾奈米碳管膜 122之奈米碳管128，在本實施例中，經由宓（pyrene)及 葱（anthracene)的物理吸附方法，可使奈米碳管128係 具有瑟及蒽的功能性修飾，另外，亦可藉由硝酸進行化 學修飾來使奈米碳管128具有親水的羧基修飾。 综上所述’因依細胞定向培養系統、細胞定向培養基板 及培養定向細胞的方法，係藉由铺設至少一奈米碳管膜 於基底上用以種植細胞，藉由奈米碳管膜中平行排列的 ^ 奈米碳管’種植於其上的細胞係可沿奈米碳管生長而達 到定向的目的’相較於習知技術，應用於受損細胞的再 生長’可導引細胞依尋一特定方向生長，進而達到細胞 修復的作用。 本發明符合發明專利要件，爰依法提出專利申請。惟， 以上所述者僅為本發明之較佳實施方式，舉凡熟悉本案 技藝之人士，在爰依本發明精神所作之等效修飾或變化 ，皆應涵蓋於以下之申請專利範圍内。 【圖式簡單說明】 圖1係本技術方案較佳實施例之細胞定向培養系統的剖視 圖。 圖2係本技術方案較佳實施例之細胞定向培養系統的俯視 圖《 圖3係本技術方案較佳實施例之奈米碳管繩的製備示意圖 097130294 表單編號A0101 第10頁/共19頁 1003473157-0 136.0573 100年.12月20日修正替換頁 [0033] 圖4係本技術方案較佳實施例之培養定向細胞的方法的流 程示意圖。 【主要元件符號說明】The 14 line includes at least one cell growth factor. In the present embodiment, the preparation of the culture solution 14 can dissolve a cell growth factor (for example, a nerve growth factor or a basic fibroblast growth factor) in a biological medium, and the biological medium can be cultured. Modulated by different cell types. [0025] In step S5, a cell 2 is planted in the culture solution 14, and the planted cell line is attached to the carbon nanotube film 122. Here, the cell 2 is directionally grown along the parallel arrangement of the carbon nanotubes 128 under the promotion of the nourishment of the biological medium and the promotion of the cell growth factor. The method for culturing the directional cells in the present embodiment may further comprise the step of: providing a current signal to stimulate the cells 2, that is, stimulating the cells 2 by outputting a minute current signal by a power source during the culture process ( For example, nerve cells) 097130294 Form No. A0101 Page 9 of 19 1003473157-0 1360573 [0027] [0030] [0032] [0032] 100 years. December 20th revised for seven hundred ' To accelerate the growth rate of the cells 2. In addition, after step S3, there is further included a step of modifying the carbon nanotubes 128 of the carbon nanotube film 122. In this embodiment, the physical adsorption method of pyrene and anthracene can be used. The carbon nanotubes 128 have functional modifications of sputum and sputum, and the carbon nanotubes 128 can also be modified with a hydrophilic carboxyl group to have a hydrophilic carboxyl group modification. In summary, the method of culturing a cell-oriented culture system, a cell-directed medium plate, and culturing a directional cell is carried out by laying at least one nanotube film on a substrate for planting cells, by using a carbon nanotube film. The cell lines on which the parallel carbon nanotubes are implanted can grow along the carbon nanotubes to achieve the purpose of orientation. Compared to the prior art, the regrowth of damaged cells can be used to guide cells. Find a specific direction of growth, and then achieve the role of cell repair. The invention complies with the requirements of the invention patent, and proposes a patent application according to law. However, the above description is only the preferred embodiment of the present invention, and equivalent modifications or variations made by those skilled in the art of the present invention should be included in the following claims. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a cross-sectional view showing a cell-oriented culture system of a preferred embodiment of the present technology. 2 is a plan view of a cell-oriented culture system according to a preferred embodiment of the present technology. FIG. 3 is a schematic view showing the preparation of a carbon nanotube rope according to a preferred embodiment of the present invention. 097130294 Form No. A0101 Page 10 of 19 1003473157- 0 136.0573 100 years. December 20th revised replacement page [0033] FIG. 4 is a schematic flow chart of a method for culturing directional cells according to a preferred embodiment of the present technical solution. [Main component symbol description]
[0034] 細胞定向培養系統：1 [0035] 基底：10 [0036] 奈求壤官陣歹】· 11 [0037] 培養層：12 [0038] 奈米碳管膜：122 [0039] 奈米碳管繩：124 [0040] 奈米碳管束片段：126 [0041] 奈米碳管：128 [0042] 培養液：14 [0043] 細胞：2[0034] Cell-directed culture system: 1 [0035] Substrate: 10 [0036] Naiqiu Nianzheng] 11 [0037] Culture layer: 12 [0038] Nano carbon tube film: 122 [0039] Nano carbon Pipeline: 124 [0040] Nano carbon tube bundle fragment: 126 [0041] Nano carbon tube: 128 [0042] Culture medium: 14 [0043] Cells: 2
097130294 表單編號A0101 第11頁/共19頁 1003473157-0097130294 Form No. A0101 Page 11 of 19 1003473157-0