TWI295373B - Disposable electrochemical sensor strip and manufacturing method for the same - Google Patents

Description

1295373 IX. Description of the invention: [Technical field to which the invention belongs]

The invention relates to a disposable electrochemical sensing test piece and a manufacturing method thereof, in particular to a measuring application which can be applied to (1) a biochemical sample, such as glucose (Glucose), cholesterol (Cholesterol), triglyceride in a fluid sample. Triglyceride, Uric acid, glycosylated hemoglobin (HbAlc), etc.; (2) Heavy metal ion detection applications, such as lead, lead, mercury (Mercury), copper ion in fluid samples (Copper), etc.; and (3) detection applications for various microanalyses. [Prior Art] In view of the increasing importance of biochemical fluid samples and heavy metal ions in various fields, the disposable electrochemical sensing test products currently used in various biochemical samples have been developed, as shown in Figure 16. One of the US Patent Nos. 6,270,637 B1 discloses a metal strip 162, 163 on a substrate 161 that is adhered to provide electrode function, and then attached to the cover sheet 164 to form a chemical-retaining reagent. Space. Another patent is shown in Fig. 16 (B). U.S. Patent No. 6,491,803 B1 'on a non-conductive substrate 167, first electrically conductive material 168, 169 such as carbon, as an electrification The electrode of the test piece is then placed on a non-conductive film material 17 〇 to provide a space for carrying a fluid sample. The two patents mentioned above all show the use of a metal surface or a printed surface of a conductive material as an electrode region 165, 166, 171, 172 for electrochemical reaction, since the electrode regions are located in the metal strip or conductive material. The surface of the electrode area is usually larger than the metal strip or the conductive material, the cross section of the 1293537 $ itself, so the measurement of the fluid sample on the electrode area is bound to result in the test. The volume of the fluid sample required for the finished product is large, so it is not ideal. How to improve the problem of excessive volume σ of the fluid sample required for the disposable electrochemical sensing test piece. After the experiment, battle and research, the inventor obtained the disposable electrochemical sensing test piece with a small electrode area and The 'process method, in addition to effectively solving the problem of excessive volume requirements of previous fluid samples, can also lead to a more simplified and minimal process for improving the electrochemical test strips. That is, the problem to be solved by the present invention is how to overcome the problem that the electrode area where the fluid sample is located is too large, so that the electrode area can be more conveniently manufactured and how to overcome the problem that the metal strip or the conductive material polar region must be separately fabricated. Improve and simplify the process of electrochemical test strips to solve the cumbersome process of traditional electrochemical test strips in the process, and to provide a small electrode area, more importantly, to simultaneously reduce the volume of the test sample, and how Overcoming the problem of how the fluid sample can smoothly reach a measurement position when flowing through the test piece. SUMMARY OF THE INVENTION The present invention is a disposable electrochemical sensing test piece comprising: - an insulating sheet - a first conductive film placed on the insulating two sheets = the first conductive film has a - first end and - a second end and - 纟: placed on the first conductive film, and the first insulating layer covers the same, and the second end forms a signal wheel, wherein the mountain is between the insulation The sheet and the first insulating layer are in the middle thereof, and a conductive cross section is used as the electrochemical sensing test piece 1, and a wide pole is used for the work of 乜95373. Preferably, the first insulating layer of the test piece is an insulating thin layer, and the insulating thin layer completely covers the first end of the first conductive film. Preferably, the second end of the test strip is a reserved conductive area that is not covered by the first insulating layer to form the signal output. And of course, the first conductive cross section exposed in the test piece can be obtained by longitudinally cutting the test piece. • Of course, the first conductive film can be placed in the test piece by one printing, one evaporation, one plating or one pasting, and the test piece has a —f two conductive film, and An insulating sheet and the middle of the first insulating layer - a second conductive cross section. Preferably, the insulating thin 4 of the (10) is a high molecular polymer, and further has a second and a third conductive film, and a second and a third are exposed between the insulating sheet and the insulating layer. Conductive cross section. And the first insulating layer of the person is a high molecular polymer, and the insulating layer has a second conductive film and a second insulating layer, and is exposed between the four layers and the second insulating layer. —Second conductive cross section.='The polymer of the test piece may be a thermosetting plastic. The basic conductive section of the test piece can be carried out by physical or The physical method used by the ιΓ圭者's wide test piece is - cutting, die, boring, water knife or - laser. The preferred method is the chemical method used for the test piece. It is a chemical residue, a 5 1295373 ginseng, a chemical decomposition or a high temperature weaving method. f H The electrode system obtained by cultivating the test piece can be - straight only less than 20/zm Ultra-microelectrode.: However, the material of the first conductive film of the test piece is one; , =, gold, enamel, nail, bell, iron, platinum, iron, recorded, recorded, water, silver or Syria. * 2 Preferably, one of the first conductive films of the test piece is made of: copper, silver, gold, rhodium, iridium, lanthanum, iron, white. , iron, nickel, Ming, water, antimony or bismuth oxides and wrong compounds. The car is the best, the test piece of the first guide Lejia gift seven mountain music and other enamel film material is a stone counter , copper, silver, gold, recorded, attacked, recorded two% of W, iron, platinum, iron, nickel, cobalt, water, antimony or silk alloy. An auxiliary electric, of course, the electrode system of the test piece can be A working electrode pole or a reference electrode. The invention is illustrated by the above concept, that is, the electrochemical sensing test piece used can be seen, and the test piece can be made by longitudinally cutting the test piece. The conductive cross section can be exposed, and has the feature that the conductive cross section is directly used as the electrode region without additionally preparing an electrode region having a larger wearing surface, and the volume of the test sample can be reduced. For ease of description, the present invention can be borrowed. The following is a description of the preferred embodiment and the accompanying drawings. [Embodiment] Referring to the first figure (AHC), a disposable electrochemical sensing test piece 10 including at least one insulating sheet is shown. a first conductive film /, its 1293537 * a second film 1 on which the first conductive film 2 has a first end 11 film 2, and a first one and a first insulating layer 3 placed on the first conductive-signal wheel end. 3 ^第:端U' and the second end of the 12-edge sound 3 is in the middle, and the end 11 of the crucible is interposed between the insulating sheet 1 and the first-thickness, and a first conductive cross section 13 is exposed therebetween as an electrochemical sense A working surface of the electrode 13 of the test piece 1 is used as an electrically insulating sheet; the first end u of the C-conductive film 2, the conductive cavity 4 guide plate' and the insulating thin layer 3 is a non-phase Moreover, it is attached to the first conductive film as a reserved conductive region, which is not connected to the signal output end by the first insulating layer. If the first Ηίη is covered, the exposed surface is exposed. 'The electrochemical sensing test piece 10 is obtained by cutting VC and the electric cross section 13 can perform a longitudinal cutting polymer on the test piece 10, and the first insulating layer 3 in the mantle (:) is - Highly divided solid plastic. The cross section is of a type of physical or chemically conductive film. The present invention extends the length of the first phase 2 in the first (Α), and directly achieves the second = 121 alignment. In this way, a cutting technique for the first conductive section 1 wide-distance test piece 10 which is not required to be processed or chemically processed, such as physics, can be obtained. A cut, a die, a drill, a water jet or an energy laser cut: a method in which the cutter exposes only the entire cut surface, and the exposed cut surface is an electric knife. The microelectrode of the present invention. The test piece 10 can also be changed to the chemical method °, / is 7 1295373 learning reaction system, -^ type. In the test piece 10, the π is decomposed or the high temperature burning is processed. The electrode 13 of one of the first conductive film 2 may be an ultra-micro 铑, 钌, 猛, iron, platinum, lanthanide, or may be a stone, copper, silver, gold, conductive film 2 - material system, , recorded, mercury, sputum or silver. The first bismuth, manganese, iron, platinum, bismuth, nickel, cobalt: t carbon, copper, silver, gold, bismuth, wrong compounds. First conductive film? , κ鈒 or New Zealand oxide and copper, silver, gold, money, Shu, = a material can also be changed to a carbon, silver or .... The electrode 13 is a two-two gold,:, recording, starting, mining, pole or a reference electrode. The first conductive film 2 is placed on the test piece 1G in the working electrode, the auxiliary electrode, and the test piece 2 in the second drawing (4) is placed on the first conductive film (4) by a printing, -_, - a method of electro-mine or adhesive bonding, and the test piece (9) further has a second conductive film 22, and exposes the first and second conductive materials as shown in the second figure (8) between the insulating sheet 241 and the first insulating layer 242. Cut off from the BM, which can be an anode (Anode) and a cathode (Cath〇de) respectively, and then attach a non-conductive film 242' and then use a cutting technique to expose only the entire cutting surface, and the exposed cutting surface electrode is The microelectrode of the invention. And in the second figure, the cross section 201 of the insulating sheet 241 and the section 211 of the first insulating layer 242 are simultaneously visible. In the first figure, the insulating sheet j of the test piece 10 and the insulating sheet 251 of the test piece 25 in the second figure (c) are a high molecular polymer, and the test piece 25 in the second figure (c) has more a second and a third conductive film 27, 28, and as shown in the second figure (D), outside the insulating sheet 251 and the first insulating layer 291, 1295373 reveals the first and second and third conductive The section 261, 271, 281, which is a mode in which the two electrodes or the above are on the same plane, and the cross section of the insulating sheet 251 and the section of the first insulating layer 291 are simultaneously seen in the second drawing (D). Referring to FIG. 2(A)-(B), the test strip 30 further has a second conductive film 6 and a second insulating layer 7 (reference numeral 8 is a first insulating layer), and the insulating sheet 4 and the second insulating layer. The first and/or second conductive sections 5a, 6a are exposed in the middle of the layer 7, in the third

Figure (c) shows the insulating sheet section 4a and the first and second insulating layer sections 8a, 7a. According to the main technical point of view, please refer to the third figure (D), the invention is a kind of disposable electrochemical ❹m piece 3, which has a conductive: special piece 32 'conductive thin # 32 has - the first The end 33 and the second end 34, a first insulating layer 35, which is placed on the first side of one of the conductive sheets 32, and the second insulating layer 37, is placed on the second side 38 of the conductive sheet 32. And the first end 34 forms a signal output end, wherein the first end 33 is interposed between the first and first insulating layers 35, 37, and the exposed-conducting section 39, = is one of the electrochemical sensing strips 31 therebetween The electrode (10)^2 has a description about the conductive sheet 32, the first and second insulating layers (10), and the descriptions of l, , and u, which are the first conductive film 2 and the first insulating layer 3 as described in the foregoing paragraph. Therefore, it will not be repeated here. If it is from another feasible point of view, please refer to the fourth figure (1). The present invention is a disposable electrochemical sensing test piece 4q, and the 47'-first conductive film 4 is placed on the chip, 47: The first conductive film 41 has a first front end 4 ιι and a second end 412, a first insulating layer is placed on the first conductive 9 1295373 film 41, and the first rear end 412 Forming a signal output end, a second conductive film 42 disposed on one of the second faces 49 of the insulating sheet 47 (in two different planes with the first face 48), and the second conductive film 42 has a first a front end 421 and a second rear end 422, and a second insulating layer 402 disposed on the second conductive film 42, and the first and second rear ends 412, 422 幵v are formed into a nickname output terminal, wherein The first and second front ends 411, 421 are interposed between the insulating sheet 47 and the first and second insulating layers 4〇1, 4〇2, and a first and a second conductive cross section 44, 45 in the outer path therebetween (detailed fourth Figure (B)), as the working surface of the electrode of the electrochemical sensing test piece, can be an anode: a cathode 44, 45, respectively. Of course, the test piece 4 at this time may further include a third conductive film 43 placed on the first face of one of the insulating sheets 47, I is exposed in the middle of the == and the 'insulating layer 401-the third conductive The carrier mode, and the other: a double electrode or more on different planes and the first insulating layer 401 #, 4lj2 is a non-conductive film, the electrode 44 45 : 6 : A: expose the entire cutting surface, exposed The cutting surface is exemplified with respect to the second and second electrodes. As for the test piece 4, the first and second insulating layers are entangled, and the insulating film is as thin as the first conductive film 2 described in the preceding paragraph. Layer 3, so it will not be described here. If the color edge sheet 1 and the first insulation are invented from another point of view, the invention is a kind of abandoning electrochemical sentence 2 'reading the fifth picture (1) and (8) 'Benedict 5', the -first conductive film 52, which has the a first conductive film 52; a front side 5 phantom and a first rear end '1295373' j * 522, a second insulating sheet 53 having a first side 54 is placed on the first conductive film 52, and the first rear end 522 forms a signal output end, and a second conductive film 55 is disposed on the second surface 56 of the second insulating sheet 53. The second conductive film 55 has a second front end 551 and a second rear end 552, and an insulating layer 57 disposed on the second conductive film 55, and the first and first rear ends 522, 552 form a signal output. The first and second front ends 521, 551 are interposed between the insulating layer 57 and the first and second insulating sheets 51, 53 with a first and a second conductive cross section 581, 582 exposed therebetween for electrification. A working surface of one of the electrode electrodes of the learning test piece, and the first one of the second one in the fifth figure (c) The sheet section 9a, 11a and the insulating layer section 14a. Of course, the test piece 50 at this time may further comprise a third conductive film (10) placed on the second surface 56 of the second insulating sheet 53, and in the second The insulating sheet 53 and the insulating layer 57 are exposed in the middle - the third conductive cross section, so the fifth figure shows a mode in which the two electrodes are on the same plane, and the insulating layer 57 is a non-conductive film, and then the domain technology is used. Exposing the entire (four) face, the cut face electrode 581, 582, 583, that is, the microelectrode of the present invention, as for the embodiment of the test strip 50, relating to the first, 52, 55, 59 ^ f - p 罘 a conductive 溥The description of the membranes 51, 53 and the details of the film is the same as the previous paragraph, he is thin, and he is thin; the inner conductive layer 2, the insulation and the first insulating layer 3, so If you look at it from another level of implementation, please refer to Tables (A) and (8). The present invention is a kind of abandonment: an analyte, which has a first insulating sheet 1293537 82, a first and a second conductive film 83, 84 disposed on the first surface 821 of the first insulating sheet 82, The first and second conductive films 83, 84 each have an electrode working end 831, 841 and a signal output end 71, 72, and an insulating layer 85' is placed on the first and second conductive films 83, 84, wherein the electrodes The working ends 831, 841 are interposed between the first insulating sheet 82 and the insulating layer 85, and between the first and second conductive sections 851, 852 and a fluid passage 73 are exposed therebetween, and the first and second conductive sections 851 are used. , a working surface of the electrode of the electrochemical sensing sheet 80, a second insulating sheet 87, which is disposed above the first and second conductive films 83, 84 and the fluid passage 73, and a cover thereof Placed under the fluid passage 73 to form a measured area of the fluid to be tested. Of course, the first insulating sheet in the first figure (1) and the insulating layer 85 each have a first groove 731 and an insulating groove plate, and the fluid channel 73 in the seventh figure is the first groove 731 and The insulating groove 7 is formed into a narrow length _ 73, and the narrow groove 73 can be formed by punching through the die. As for the test piece 7 〇 embodiment, the second conductive film 83, 84, the first The description of the other details of the second insulating sheet breaking force and the insulating sound 85 are as described in the preceding paragraph, the first conductive film 2, the insulating sheet! And the first insulating layer 3, so this is no longer the electric 4. If it is from another manufacturing point of view, please refer to the sixth figure, which is a kind of disposable electrochemical sensing test piece 6G for the body== Wide first insulating thin "4, which has = Γ: Τ conductive film 61, 62, which is placed on the placement surface 65 ί - Τ 6 ΓΓ _61, 62 each having a first end 6_ and a first known 66, 67, and one The insulating layer (10) is placed on the first and the 12th 1295373 j · ; . . , : , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , Each of the second ends 66, 67 forms a signal output end, wherein each of the first ends 611, 621 is interposed between the insulating layer 68 and the first insulating sheet 64, and a first and a second conductive section 691, 692 are exposed therebetween, and The first and second conductive films 691, 692 are used as the working surface of one of the electrodes 691, (10) of the electrochemical sensing test piece 6 . Of course, the first and second conductive films 61, 62 of the test piece 6 系 are borrowed. Made of -printing, -steaming, an electric ore or a pasting method, and the test piece 60 has a circular opening 63, as for the first embodiment of the test piece 6 The descriptions of the second conductive _ 61, 62, the first insulating sheet 64 and the other insulating portions of the insulating layer 68 are the first conductive film 2, the insulating sheet i and the first insulating layer 3 as described in the foregoing paragraph. If the circular opening 63 of the test piece 60 is changed to the elongated groove 73 in the seventh figure, the first and second conductive films 61, 62 in the sixth figure are equivalent. The first and second conductive films 83, 84 in the eighth figure, and as shown in the eighth figure, have a fluid passage 73 in the insulating layer 85 and the first insulating sheet 82, and the test piece 70 further includes - The second insulating sheet 87 is disposed above the first conductive film, the second conductive film 83, 84 and the fluid channel 73. The test piece 7 further includes a cover sheet 81 placed under the fluid channel 73 to form One of the fluids to be measured is measured, and the test strip 7 further includes a chemical reagent 86 disposed in the fluid channel 73 for chemically reacting with the analyte, and the electrodes 851, 852 and the second ends 71 72, an electronic measuring signal is outputted to the test piece 70, the fluid to be tested is a sample, and the fluid channel is 3 lines, and one of the samples is In the eighth figure (B), it can be seen that a vent hole 89 is left in the bottom surface of the test piece 7 , to facilitate the flow of the fluid to be tested in the fluid passage 73. 13 1295373 The physical mode used for the test piece 60 is one. Cutting f, soil die: a hole in the water knife or laser, and the blades 60, 70 in the sixth, seventh and eighth figures can be used to form the electrode surface 691, 851 by cutting technology. The fluid passage 73 and the space 63 can be formed, and the space 63 can adsorb a chemically modified material such as an electrochemical transfer substance, a redox reactant, or the like, that is, a source of the signal source for detecting the test pieces 60, 70. Furthermore, if the circle y opening 63 of the test is changed to the center opening of a disk in the ninth figure (β), the first and second conductive films 61, 62 in the figure. That is equivalent to the second picture? In the first and second conductive films 91, 92, and the first and second conductive sections 96, 97 of the test piece 9 are processed by physical or chemical means, as shown in the ninth The first insulating sheet 95 and the insulating layer 93/ shown in (Α) are respectively a non-conductive substrate 95 and a disc-shaped non-conductive thin sheet 'and the first and second conductive films 91, 92 and other plurality of strips The conductive film is above the electrode), and is applied to the non-conductive substrate 95 in a radial form, and the insulating layer (10) is attached to each of the first and second conductive films 91 92 6 (Fig. 6) It can be clearly seen that the first and second conductive sections ', position, the series of independent and electrically conductive materials 91, 92 in the same plane mode is the electrode of the disk array, and the cutting hole is used The technique of exposing the entire bore cutting surface 96 is the microelectrode of the present invention. The cut-off electrode 96 of the path, the circular opening 63 of the test piece 60 can be modified to include the end insulating layer as in the tenth-figure (1) I: and the two-St 119' test piece 110: The sheet 118 is formed to form an elongated channel 119' which is the measurement area 4 of the fluid to be tested, and then the first and second guides 14 c S- , 129.5373 61, 62 and the second end (10) in the sixth figure, 67, the insulating layer 68, the first and second conductive sections 691, 692 are equivalent to the first and second conductive films 113, 114 in the eleventh figure (a), the second end 111112, the insulating layer 115, the first and the The two conductive sections 117, 116, and the elongated channel 119 has a fluid inlet port 111 and a venting opening 1111. And the eleventh figure (A) end insulating layer 1112 can be omitted, and changed to the elongated channel 129 of the test piece 12〇 in the eleventh figure (B), then the tenth-graph (A) 1. The second conductive film 113114, the second ends 111, 112, the insulating layer 115, the first and second conductive sections 117116 and the cover sheet 118 are equivalent to the first and second conductive films 123 in the eleventh figure (8). '124, second end 121'122, insulating layer 125, first and first conductive sections 127, 126 and cover sheet 128. Brother and brother, a conductive v-foot shell, her dust and sad look, as shown in the first figure (A) _ (c) j: 'Ben Mao Ming is a disposable electrochemical test piece 1 包括 = include ^ next step 'provide at least —Insulation sheet is placed on the ground < Place a first conductive film ^, / / piece

= the second end 12' is placed - the first insulating layer 3 is on the first conductive film 2 and the 苐-insulating layer 3 covers the first end u, and the second:: film 2

, guiding and separating from the first end η, the seizure causes a first lead two T-die to press through, one drill;: the cubit can be cut by light-cutting, the crystallization and the eight-grain , a chemical etching, if you are enough to solve the problem and burn in a warm manner. Ming Nai (4) - a manufacturing level, please refer to the third * * 乃 乃 种 种 种 种 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学 电化学One of the fluids, comprising a step of providing a first insulating sheet 64 having a placement surface 65 on which a first and a second conductive film 61, 62 are disposed. And the second conductive films 61, 62 each have a first end 611, 621 and a second end 66, 67, and an insulating layer 68 is disposed on the first and second conductive films 61, 62, and the insulating layer 68 is covered. Each of the first ends 611, 621, and each of the second ends 66, 67 forms a signal output end, and separates the first ends 611, 621, and obtains a first conductive section 691, a second conductive section 692, and first The conductive section 691 and the second conductive section 692 are divided into a working surface of an electrode 691, 692. As shown in the tenth figure, it is an embodiment of an electrochemical cyclic voltammetry (Electrochemical Cyclic Voltammetry), that is, a microelectrode structure test piece 10 obtained by the cutting technique of the present invention (detailed first figure (C) )), which can utilize an electrochemical analyzer and its apparatus in combination with a reference electrode 10b and a counter electrode 10c for measurement of a biochemical specimen. By using the microelectrode 0 13 structure, the diffusion phenomenon of the reactants can be reduced on the test strip 10, so that the electrochemical reaction behavior is more obvious. As shown in the twelfth and thirteenth figures, the microelectrode test results of the present invention (ie, the twelfth map) were performed using 3 mM iron cyanide (ferricyanide) at a scan rate of 50 mV/s, respectively. The results shown are compared with the test results of the existing general electrode (ie, as shown in Figure 13). Further, in an embodiment of the present invention for measuring the concentration of glucose in a blood sample of a human body, it can be carried out in the following manner: · · Electrode material: working electrode (Au) - auxiliary electrode or reference electrode (Au) 16 1295373

b·applying potential: 〇. 2V c·chemical ratio applied to the electrode): reagent is (this is after the moisture is dried)

24% 20% 9% 1% 46% dihydrogen dihydrogen diphosphate sodium dihydrogen phosphate glucoside oxidase

Microcrystalline hydrocarbyl ethyl cellulose potassium ferricyanide

e•Comparative instrument: YSI analyzer f•Test sample: glucose solution g·sample range: 2~3〇mM h·Test result: as shown in Figure 14. Further, if the heavy metal ion detection of the present invention is to be carried out, it can be carried out in the following manner: a·electrode··working electrode (w〇rking)_the microelectrode structure test piece of the present invention is coated with a mercury film; b·the auxiliary electrode ( Counter> Platinum electrode; c• Reference electrode (Reference)_Ag/AgCl; d· Solution composition: 〇·ιν nitric acid; e. Can be used with Square wave voltammetry (Square_wave Anodic Stripping Voltammetry, SWASV) to detect 20 and 50 ppb of the wrong ion; f. The result is as shown in the fifteenth figure. In summary, the present invention can be performed in a novel design by performing a longitudinal cutting of the test piece. The test piece can expose the conductive cross section, and the direct use of the conductive cross section as the electrode area can obtain 17,1295373, and the electrode area having a larger cross-sectional area can be additionally prepared, and the volume of the test sample can be reduced. Therefore, anyone who is familiar with this skill can be modified by the ingenuity, but they are not protected by the scope of the patent application. _ [Simple description] _ First picture (A)-(C ): is the disposable electrochemical of the present invention The exploded perspective view, the combined perspective view and the front view of the preferred embodiment of the test piece; the second figures (A)-(D): the other two of the disposable electrochemical sensing test pieces of the present invention BRIEF DESCRIPTION OF THE DRAWINGS FIG. 3(A)-(D) is a perspective exploded view of a preferred embodiment of the disposable electrochemical sensing test piece of the present invention, a combined perspective view, and a front view BRIEF DESCRIPTION OF THE DRAWINGS FIG. 4(A)-(B) is a perspective view and a front view of another preferred embodiment of the disposable electrochemical sensing test piece of the present invention. Fig. 5(A)-(C) is a perspective view of the disposable electrochemical sensing test piece of the present invention, a perspective view of a preferred embodiment, a combined stereoscopic view, and a front view, and a sixth figure. FIG. 7 is a perspective view showing another preferred embodiment of the disposable electrochemical sensing test piece of the present invention; FIG. 7 is a perspective view showing another preferred embodiment of the disposable electrochemical sensing test piece of the present invention; Eight Diagrams (A)-(B): It is the test piece and the second one in the seventh picture. A perspective view of the back side of the combination of the sheet and the cover sheet and a perspective view of the popping; ninth drawing (A)-(C): is the disposable electrochemical sensing test piece of the present invention 1295537 w » ...... Further, the exploded perspective view of the preferred embodiment, the combined "body" and the three-dimensional enlarged view of the central portion; _thth: the test piece in the first picture is combined with the reference electrode and the auxiliary electrode for biochemical examination. FIG. 10(A)-(B) is a combined perspective view of still another preferred embodiment of the disposable electrochemical sensing test piece of the present invention; A diagram showing the relationship between voltage and current of the electrochemical reaction behavior test of the microelectrode of the present invention using iron cyanide; Fig. 13 is a diagram showing the relationship between voltage and current for conducting electrochemical reaction behavior test of a general electrode using iron cyanide; , agriculture is a measure of the glucose concentration in the human blood sample test / Chen and current relationship diagram; Figure 15: is the relationship between the weight and current of the present invention; the voltage slice taken with & butterfly ion ==(AMB): It is a common discard of two kinds of abandonment The electrochemical sensor strip of explosive perspective view of the opening. [Main component symbol description] 10, 10a: Electrochemical sensing test piece 1〇b: Reference electrode 10c: Auxiliary electrode 1: Insulating sheet First conductive film la: Cross-sectional end 12 of insulating sheet: Second end 3: First-insulation Layer/insulating thin layer 121: section 13 of the first longitudinal edge layer: first conductive cross section, (micro)electrode V longitudinally cut electrochemical sensing test piece 19 cs 21 : first conductive film 22 : second conductive Film 24: second conductive section 242: first insulating layer/non-conductive film 251: insulating sheet 27: second conductive film 261: first conductive section 281: third conductive section 4: insulating sheet 6: second conductive film 12 tear 71 face 211 : section 23 ··first conductive section 241 : insulating sheet 25 : electrochemical sensing sheet 26 : first conductive film 28 : third conductive film 271 : second conductive section 291 : first insulating layer 8 : First insulating layer 5a: First conductive section 7a: Second insulating layer section • 5: First conductive film 30: Electrochemical sensing test piece 7: Second insulating layer 4a: Insulating sheet section 6a · Second conductive section 8a: First and second insulating layer sections 31: Electrochemical sensing test piece 32: Conductive thin, 33: first end 35: · a first insulating layer 37 second insulating layer 39: conductive cross-section 401: a first insulating layer 402: second insulating layer 411: first front end

34: second end 36: first face 38: second face 40: electrochemical sensing test piece 41: first conductive film 412: first rear end 42: second conductive film 421: second front end 20 1295373 » _ ' 422: second rear end 44: first conductive section 43: third conductive film month> heart one 45: second conductive section 46: third conductive section 47: insulating sheet 48: first side 49: second side 50 : electrochemical sensing test piece 52 : first conductive film 51 : first insulating sheet 521 : first front end 522 : first rear end 53 : second insulating sheet 54 : first surface Lu 55 : second conductive film 551 : Two front ends 552: second rear end 56: second side 57: insulating layer 581: first conductive section 582: second conductive section 583: third conductive section 59: third conductive film 9a: first insulating sheet section 11a: Second insulating sheet section 14a: insulating layer section 60: electrochemical sensing sheet 61: first conductive film 62: second conductive film 611, 621: first end 63: circular opening/space 64: first insulating sheet 65 : placement surface 66, 67 · · second end 68 : insulating layer 691 : first conductive cross section / electrode (face) 692 : second guide Cross section/electrode 70: electrochemical sensing test piece 71, 72: signal output end / second end 73: fluid passage / slit groove 731: first groove 732: insulating groove 81: cover sheet 82: first insulating sheet 821: first surface 83: first conductive film 84: second conductive film 21 1295373: ^^ pole end / first end (1) insulating layer ^ electrical cross section / electrode (face) 852: Chu -, 86: chemical reagent 87: The first edge of the factory conductive surface / electric branch 89: exhaust hole brother - insulation 4 pieces of Anji Q1. ^ 90 · electrochemical sense test 91. The first conductive film 92 · · second conductive film 93 ··Insulation layer 守 qs · Chu all 94 : disc center opening •-,,, slab sheet / non-conductive substrate 96: first conductive section / cutting surface / electrode 97 110: Electrochemistry _ (9) mm Conductive section 113, 114: second conductive film 116: second conductive section 118: cover sheet 1110: fluid inlet port 1112: end insulating layer 121, 122: second end 125: insulating layer 126: second conductive section 129: Long and narrow channel 162, 163: metal strip 165, 166: electrode region 168, 169: conductive material 171, 172: electrode region v6

115: insulating layer 117: first conductive section 119: narrow channel 1111: vent hole 120 • electrochemical sensing test piece 123, 124: first dolphler-conductive film 127: first conductive section 128: cover piece 161 · substrate 164 : Cover Sheet 167 : Substrate 170: Branch of the Electric Power Material 22 C S5

Claims (1)

.1295373 ..,.: ., + β _____w - , Patent Application PCT · A disposable electrochemical sensing test piece comprising: at least one insulating sheet; - a first conductive film placed on the insulation On the sheet, the first/conductive film has a first end and a second end; and a first insulating layer disposed on the first conductive film, and the first insulating layer covers the first And the second end forms a signal wheel end, wherein the first end is interposed between the insulating sheet and the first insulating layer, and the exposed-first conductive cross section is exposed therebetween as the electrochemical sense test A working surface of the two electrodes of the chip. 2. If the scope of the patent application is a thin layer of insulation, the first end. The test piece according to Item 1, wherein the first insulating layer and the insulating thin layer completely cover the first conductive thin film & the test piece according to the patent application specification - Reserved conductive area, which is not the :, - is the signal output. The brothers and the shackles are covered by the shackles to form 4. If the patent application scope is reached! The item described in the item is for the rhyme film - the vertical film is obtained by a printing, tomb, /, middle placement of the first | The test piece further has a second conductive-plating or-adhesive manner, an insulating layer is exposed in the middle of the first to third, and the insulating sheet and the body are electrically conductive. 6) If the scope of the patent application is a high molecular polymer, and the ' ^ ' piece, wherein the insulating sheet has a second and a third 23 1295373 film, and the insulating sheet and the The first insulating layer is exposed in the middle and a third conductive cross section. A test piece according to claim 1, wherein the first insulating layer = -μ subpolymer ' and the test piece further has a second conductive film and a first insulating layer, and the insulating film is thin , a second conductive cross section. 4 pieces and (4) two insulating layers are exposed in the middle. The test piece described in Item 6 or Item 7 is the high-strength plastic or thermosetting plastic. 9. If the test piece surface described in the scope of the patent application is processed by physical or chemical means, the conductive cut-off is obtained as described in item g of the scope of the patent application - cutting, - die The way to drill a hole is one of the ways to apply for patents. It is a chemical-based, medium-track learning system. A knife solution or a flame burning method. 12. The electrode system according to claim 9 is an ultramicro electrode. After processing, pass 13·If the application of the patent film is - the material is a carbon, copper and silver, the first - conductive thin gold, moth, record, aunt, mercury: two gold, money, ", iron, white The test piece described in the film "material", wherein the first conductive thin platinum, hungry, recorded, * s stone reverse, copper, silver, gold, bismuth, antimony, manganese, iron, 15 • patent application /, mining, silver secret oxides and wrong compounds. The test piece described in the track 1 of the track, wherein the first conductive thin 24 1295373 T - the material is a carbon, copper, silver, gold, money, Gold, gold, antimony, nickel, abundance, mercury, silver or alloy of the secret. Shenji please -^1!1:*, the + material is 4 auxiliary electrode or a reference electrode. A disposable electrochemical sensing test piece having: a first insulating layer disposed on one of the conductive sheets; 2: an electric sheet having a first end and a second end and a first The insulating layer is placed on the first signal output end of the conductive sheet, wherein the first end is: the: the chemical-sensing test piece--the electrode of the electrode. f is the material: two The test piece described in the scope of patent application ,17, 9: by: printing, - steaming, - electric ore or - pasting: made of the test piece as described in claim 17 of the patent scope, into ° The second insulating sheet is a high-molecular polymer. The medium and the polymer are | wherein the conductive cut-off is as described in claim 19. Or - thermosetting _, :, such as: 1 The test piece 1 described in Item 17 of the patent scope is a physical or chemical method 22. If the processing is in the second part of the patent, it is a cutting, Syrian film, the physical side of the two sides of the two knives, a hole, a skin π seven one + & 23. As claimed in the scope of the 21st item $ laser. Department of - _ _, - silk The test piece of Μ 34, wherein the chemical is a chemical decomposition or a high temperature combustion. The method of claim 21, wherein the processed piece/electrode is an ultramicroelectrode. The test piece described in item 17, which + the conductive sheet polymer is a genus vermiculite f: conductive carbon, conductive plastic 谬, conductive cobalt, fruit, 鈒Μ of the sheet. A fierce iron, platinum, osmium, nickel, For example, one of the materials mentioned in the scope of the application is a magnetic, plus, and, etc., gold, hungry, nickel, cobalt, silver, gold, strontium, barium, manganese, iron. , white 27:, water, bismuth or antimony oxides and wrong compounds. 27. For example, one of the patent-originated milks is a test piece of Good Zhewei & Xiang Jinju, in which the conductive 孳 only ί, Γ:: contains broken 'copper, silver, gold m :: 9t hungry, recorded, Ming, An alloy of mercury, strontium or island. Iron white 28. As described in claim 17, the working electrode, the auxiliary electrode or the reference electrode is a 9.-disposable electrochemical device, which is an insulating sheet; a conductive film 'which is placed on the top, and the first conductive film has a first 1-sided-first insulating layer, which is placed at the first end; a rear end forms a signal output; On the film, and on the first brother's - conductive film, the agricultural film, and the second conductive film and it is placed: the second side of the insulating sheet and the electric phase have a second front end and a second rear The first insulating layer is disposed on the first insulating layer, and the second rear end forms a signal on the electric film, and the first front end is interposed between the first and second first and second Exposed in the middle - the first and the second insulating layer, and the upper conductive cross section of the electrode of the test piece as the electrochemical sensing = · as in the 29th phase of the patent application, it is placed in The second (four) one/one package 3 a second guide sheet and the first permanent/first surface, and the insulation - and the second guide exposed a third conductive cross section, wherein the first* electrical phase is made by printing, vapor deposition, electro-minening or a pasting method, and the test piece described in the present invention Wherein the insulating sheet: ι: the second insulating layer is a high molecular polymer. The test described in item 31 of the patent scope is - heat New Zealand or _ thermoset transfer. 〃 | t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t t It is a chemical money engraving method, such as the test piece described in the patent scope of $33, wherein the physical mode % cutting, punching, drilling, waterjet or laser. / The test piece according to claim 33, wherein the chemical mode, the chemical decomposition, or the high temperature combustion is a genus. The test piece described in claim 33, The electrode obtained by processing is an ultramicro electrode. 3? The test piece according to claim 29, wherein the first and the 27 1295373 second conductive film are made of manganese, iron, platinum, iron, zinc, steel, silver, gold , 铑, 钌, 38. If you apply for the scope of the patent, the table or the second. The material of the second conductive film is _: = sheet = the first and the nail, manganese, iron, platinum, hungry, nickel, cobalt, =, 5 silver, gold, bismuth, wrong compound. An oxide of water, hydrazine or hydrazine and 39. as described in claim 29 The second conductive film - material A '" film 'eight in the first and the first, 猛, fierce, iron, platinum, moth two;, 'copper: silver, gold, money, 40 such as the Shenshi main law f円 knot, ^铱 or alloy of New Zealand. For example, a working electrode, an auxiliary electrode or a reference electrode of the 29th patented dry brother is 41. A disposable electrochemical sensing test piece, which has a first insulating sheet; :drr is placed on the first insulating sheet, the first conductive film has a top end and a first end. A second insulating sheet has a '', a (four) film: and the first back end Forming - the signal is placed on the first surface of the yfi conductive film, and the second insulating film disposed on the second insulating sheet has a second front end and a second rear end; And a layer disposed on the second conductive film and forming a signal output end, wherein the first and the first edge layer are in the middle of the first and the second insulating sheet: The first and second conductive cross sections serve as a working surface of the electrode of the electrochemical 28 1295373 sensing test piece. 42. The test piece of claim 41, further comprising a film as disposed on the second side of the second insulating sheet, and the edge sheet and the insulating layer are exposed in the middle - a three-conducting cross section, wherein "- and the second conductive film are formed by printing, frequency, or a paste. The test piece described in item 41 of the electroplating range, and the first The insulating sheet and the insulating layer are at a height of 44. According to the patent application, the 43th wide compound is - thermoplastic plastic or a thermosetting = 'where the polymer is a compound described in item 41 of the eleventh, " Conductive _: The residue is obtained by physical or chemical processing. m 47. If the patent application is 45th = the way of shooting. The system is a chemical _ ~ light _ a ^ = its (four) chemical way. For the knife solution or a warm combustion 48. The electrode is the same as the ultra-micro electrode. 〃"片' which has been processed and 49. If the second conductive film of the fourth paragraph of the patent application scope is - material: -: / piece 'the first and the radiance, iron, platinum, iron, nickel , cobalt, =, copper, silver, gold, bismuth, bismuth, 50 ^ ^ 铱, 铱 or 铋. 〇. For example, the material of the second conductive film of the 41st patent scope of the Shenqing patent, the two-piece film, the t, the first, 3 carbon, copper, silver, gold, bismuth, 29.1295373 钌, fierce , the wrong compound platinum, hungry, nickel, cobalt, mercury, antimony or antimony oxide and = · as claimed in the patent application (4) 41, wherein the first and the first conductive film - the material is - Alloys containing carbon, copper, silver, gold, ruthenium, osmium, manganese, iron, platinum, hungry, nickel, cobalt, mercury, ruthenium or osmium. 52. The test piece according to claim 41, wherein the electrode is a working electrode, an auxiliary electrode or a reference electrode. A disposable electrochemical sensing test piece for testing an analyte of a fluid to be tested, comprising: a first insulating sheet; a first conductive film and a second conductive film disposed on the first insulating sheet On the first side, the first and second conductive films each have an electrode active end and a signal output end; an insulating layer is disposed on the first and second conductive films, wherein each of the electrodes Between the first insulating sheet and the insulating film, a first and a second conductive cross section and a fluid passage are exposed, and the second conductive cross section is used as one of the electrochemical sensing strips. a working surface of the electrode; a second insulating sheet disposed above the first and second conductive films and the fluid passage; and a cover sheet placed under the fluid passage to form the fluid to be tested One of the measurement areas. The test piece according to claim 53, wherein the first and the first conductive film are formed by a printing, vapor deposition, electroplating or a pasting method of 1295373. 2. The test piece according to claim 53: the insulating layer and the second insulating layer are thin, insulated, and the first insulating sheet and the insulating layer are each: a knife polymer, a groove, and the fluid channel is The first groove and the - one slit are insulated. ^,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, The conductive cross section is obtained by processing - physical or - ° ' ', the first and the 58th, as described in claim 57 of the patent application. It is a cut, a die, and a 4 piece of ^ which is the physical mode 59. As claimed in the 57th item: knife or - laser. It is a chemical-remaining, optical-patterned film, in which the chemical mode. Χ A chemical decomposition or a high temperature combustion 60. The electrode is obtained as an ultramicro electrode as in the 57th patent application. The test piece of the catty, which has been processed and 1 · such as the second conductive thin test piece of the patent scope, the first and the seam, iron, platinum, hungry m, copper, silver, gold, enamel, nail, This is as in the scope of patent application No. 53: Silver repair. The second conductive film-material, the test piece, wherein the first and the seam, iron, platinum, bismuth, and zinc are two carbon, copper, silver, gold, money, nail, and compound. Oxide and mis-distribution of station, ice, bismuth or antimony 31.1295373 =·If the test piece described in _53, the material of one of the conductive films is a carbon, copper, silver, gold, Money and "alloys of iron, platinum, nickel, diamond, mercury, antimony or silver. Used as an electrode, an auxiliary electrode or a reference electrode. Still. As for the K piece of the AW item of the patent application, the towel (4) (4) As a sample, and the fluid channel is one of the inlet ports of the sample. A disposable electrochemical sensing test piece 'for testing—one of the analytes to be tested, comprising: a first insulating sheet having a And a first conductive film disposed on the placement surface, the first and second conductive films each having a first end and a second end; and the t and the edge layer are disposed Putting on the first and the second conductive film, the edge: covering each of the first ends' and each of the second ends forms a signal output U. The first end is interposed between the insulating layer and the first insulating layer In the middle of the sheet: a first and a second conductive cross section are exposed therebetween and the first and second conductive portions are exposed The test piece of the electrode of the electrochemical test piece, wherein the insulating layer and the first insulating sheet have a fluid passage, and The test piece further includes a second insulating sheet disposed on the first conductive film, the second conductive film, and the fluid channel. 68. The test piece according to claim 67, wherein the first The insulating sheet, the insulating layer and the material of the second insulating sheet are a high molecular poly 32 ',1295373 compound. 69. The test piece according to claim 67, further comprising a cover sheet, which is disposed Disposed under the fluid channel to form a measurement region of the fluid to be tested, and the test piece further comprises a chemical reagent disposed in the fluid channel for chemically reacting with the analyte The electrode and each of the second ends outputs an electronic measuring signal. The test piece according to claim 69, wherein the cover sheet is a non-conductive high molecular polymer. Patent application section 68 or 7 The test piece according to the item, wherein the polymer is a thermoplastic or a thermosetting plastic. 72. The test piece according to claim 67 further comprises a chemical agent, which is placed in the fluid. In the channel. = The test piece described in claim 72, wherein the chemical reagent I: contains an enzyme, an antigen, an antibody, an electrolyte, a thickener, a water or a biochemical protection agent. The test piece of claim 66, wherein the first and the second conductive film are made by a printing method, and the test piece has a circular opening. The test piece according to the item, wherein the first and second of the first--th sheet and the insulating layer are == two:, and the film of the disk type, and the first and the η a non-conductive substrate and the -round The test piece described in claim 75, wherein the physical mode 33 1295373 water knife or a laser is a cut, A die, a drilled hole, and the test piece has a disk center opening. 77. If the scope of the patent application is the test piece of the chemical chemistry, the chemical method. First etching, - chemical decomposition or - high temperature combustion 78 · as claimed in the scope of the 75th The electrode system is - ultra-micro electrode / Syria W, which is processed and 7th 9th, such as the second film _ conductive film, one of the materials is - carbon, and the cover - cover is replaced by 幵: , 铱 or silk, and the test piece is more domain. 40%-long and narrow channel, which is the test piece of the fluid to be tested-80. According to the patent application, the test piece of the 79th second guide, the test piece described in the item, wherein one of the Vth electric film The material is - violent, iron, platinum, bismuth, bismuth, C, copper, silver, gold, recorded, 』, and the test piece contains I silver or money oxide and attack 81 ^ insulation layer ' Forming the household, base, 81 · as claimed in the scope of the 66th «, 丨 < body through 3 second conductive film material is a fascinating test piece 'the first manganese, iron, platinum, hungry , nickel, cobalt, bismuth, copper, silver, gold, bismuth 82. If the silver or bismuth alloy is used in the 66th section of the patent application. - one of the electrode sets of the working electrode, the test piece of the auxiliary fan' wherein the electric (four) 83. such as a patented electrode or a reference electrode. A sample of 67 items is found in the case of Ai Mingli, and the fluid channel is a wide-ranging electrochemical test. The manufacturing method of the month, comprising: providing at least one insulating sheet such as τ 34 1295373; H placing a -first conductive thin crucible on the insulating sheet, the first leading-first end and the second end; The pancreatic device has a discharge-first insulating layer on the first conductive film, and the first insulating layer covers the first end, and the second end forms a signal output end; and ... separates the first end, A first conductive cross section is obtained, and the first surface is a working surface of one of the electrodes of the electrochemical sensing test piece. Battle: Second application: The method described in the 84th patent range, wherein the separation of the test piece is from - cutting and cutting, and punching through a die, ::: - chemical decomposition 2:: 86. A method for manufacturing a test piece for testing a fluid to be tested, comprising: providing a sensation-providing-first insulating sheet having a placement surface; the first and the mounting surface a first and a second conductive film: each of the first and second ends; and the first end of the first and the second side of the ,-道恭:, 夂:: An insulating layer is disposed on the film, the insulating layer is separated from the first ends, the two ends form a signal wheel end; and the surface, and the first guiding +: a first conductive wearing surface and - second Conductive section-working surface ^ section, the second conductive section is 1 pole of 35 1295373 C s 5 1295373 Voltage / Volt Twelfth Figure % 1295373 tenth 1295373 -1.6e-5 -1.0e-5 -1.2e-5 -1.4e-5 -5-5 ee- 8 o -1··2· L -1.0e-5 -i 20 ppm Pb2+ -1.2e- 5 - -1.4e-5 - / - 广-1.6e-5 - / • {Cup-1·8θ·5 - \ Μ 1® -2-〇e-5 - _2.2e-5 - - -2.4e -5 - y - -2.6e-5 - III -2.8e-5" 1111 -0.4 -0.5 -0.6 -0.7 -0.8 -0.9 -2.2e-5 -2.4e-5 -2.6e-5 -2.8e-5 -0.4 -0.5 -0.6 -0.7 -0.8 Voltage / volt / volt 15th picture 1293537' #毒五, Chinese invention summary: fV 4 > The present invention is a disposable electrochemical sensing test piece and a method of manufacturing the same. The test piece includes at least one insulating sheet, a first conductive film placed on the insulating sheet, and the first conductive film Having a first end and a second end, and a first insulating layer disposed on the first conductive film, the first insulating layer covering the first end, and the second end forming a signal output The first end is interposed between the insulating sheet and the first insulating layer to expose a first conductive cross section therebetween as a working surface of one of the electrodes of the electrochemical sensing sheet f. A disposable electrochemical sensor strip and a manufacturing method for the same are provided. The disposable electrochemical sensor strip includes at least an isolating sheet, a first conductive film mounted on the isolating sheet and having a first end and a second The second end forms a signal output terminal and the first end is between the isolating sheet and the first isolating layer for exposing a first conductive section to be a working surface of an electrode of the electrochemical sensor strip. VII. Designated representative map: (1) The representative representative of the case is: (1) Figure (C). (2) A brief description of the symbol of the representative figure: :: absolutely, thin film 20 conductive film 3: first insulating layer / insulating thin reed la · insulating sheet section 10 : electrochemical sensing test piece 曰 11: first end 12: second end Η , & electrical cross section / (by) electrode 3a · first insulating layer VIII, if there is a chemical formula in this case, please reveal the most characteristic of the invention &