TW557201B - Animal feed additives and method of producing the same - Google Patents

Abstract

The present invention provides a method of producing animal feed additives which improve an environment of enteric bacteria, suppresses resistance bacteria and enhances the quality of animal products. That is, the present invention provides a technology for improving the environment of enteric bacteria of livestock to create a sound enteric environment, resulting in healthy excretions, compressing pathogenic bacteria, and enhancing animal product quality and productivity, thereby reducing expenses for managing a farm in general. The animal feed additives are prepared by adding an antioxidant and a viable cell agent to a high quality organic substance and fermenting the mixture in a sealed and air permeable container at a temperature range of from 25 to 37 DEG C for a certain period of time.

Description

557201 V. Description of the invention (1) [: [Application scope in industry] The present invention relates to the addition of antioxidants and viable agents (composite culture of viable bacteria by lactic acid bacteria and yeast, or lactic acid bacteria, yeast and acetic acid) to advanced organic substances. (Compound culture of live bacteria and photo-synthetic bacteria is preferred), a feed additive manufacturing method for the purpose of manufacturing feed additives by the action of antioxidants and fermentation of live bacteria. [Previous Technology]

The environmental problems caused by the stench of livestock farms and chicken farms are deeply affected by farmers, local residents and livestock. On the one hand, the farmer cannot live in an isolated environment from outside the local residents, but also introduces a high price to alleviate the malodor: an oxygen generator, and uses various anti-biomass to suppress diseases of livestock. For this reason, a vicious cycle of newly resistant bacteria in livestock is caused. All of these threaten farm management every year. [Problems to be Solved by the Invention] Therefore, a technology for improving the environment of intestinal bacteria in livestock and suppressing financial bacteria, both to improve the quality of animal products and the environment of intestinal bacteria, that is, to improve the environment of intestinal bacteria in livestock To form a sound intestinal environment, to achieve the improvement of feces and urine, the suppression of pathogenic bacteria, the suppression of malodor and flatulence, the improvement of the quality of animal products and the improvement of animal productivity, and a feed additive that promotes a reduction in farmer operating costs and a method for manufacturing the same .

[Methods used to solve this problem] The inventors have added antioxidants and live bacteria (complex culture of live bacteria with lactic acid bacteria and yeast, or compound culture of live bacteria with lactic acid bacteria, yeast and acetic acid bacteria) to advanced organic substances. , And photosynthetic bacteria are better), making it

Page 4 557201

V. Description of the invention (2) I Research on the manufacture of feed additives by fermentation. As a result, it was found that the fermented feed ^ additive richly contained proliferating live bacteria and amino acids, organic acids, vitamins, nucleic acids and the like generated from the live bacteria. It will become a nutrient, which will grow the bacterial stomach in livestock (especially in the intestine) and regulate it into a sound intestinal environment. That is, the present invention is to add anti-oxidants and live bacteria to high-grade organic matter, add it to a container in a closed aeration environment, and ferment it at a temperature of 25 ° C to 37 ° C to produce a feed additive. The moisture content after adding anti-oxidant substances and live bacteria agents to advanced organics is more suitable to be set at 15 ~ 25% by weight. In the manufacturing method of the feed additive of the present invention, the excessive oxidation of high-level organic substances is suppressed due to the antioxidant properties of the organic matter. On the other hand, the lactic acid of the substance produced by the lactic acid bacteria in the live bacteria agent makes the mixed bacteria Suppressed. In addition, as described below, aerobic bacteria attached to the air and materials, or aerobic bacteria (acetic acid bacteria) and general aerobic bacteria (lactic acid bacteria, yeast) that can be contained in live bacteria agents, Oxygen consumption, and the carbon dioxide generated by them, inhibits the auto-proliferation of these aerobic bacteria and aerobic bacteria, and the carbon dioxide can be utilized by photosynthetic bacteria. Therefore, even if the feed additive manufacturing method of the present invention is not subjected to heating treatment of high-level organic matter, it can not only inhibit miscellaneous bacteria in a container (such as a plastic bag) in a closed and aerated environment, but also proliferate and ferment the living flora. In step φ, amino acids, organic acids, vitamins, and nucleic acids are generated as bacterial nutrients in the intestine. [Embodiment of Invention]

Page 5 557201 V. Description of the invention (4) Furthermore, the photosynthetic bacteria are also freeze-dried bacteria (preferably) inoculated in an appropriate medium, cultured and obtained 3 nutrients). In the case where a plurality of types of photosynthetic bacteria are used, it is preferable that 40% of the bacteria are cultured in individual culture liquids and then mixed. In the case where acetic acid bacteria are used to add the above bacteria, the freeze-dried body is inoculated in an appropriate medium, cultured and obtained, and then the culture solution is prepared. In the method for manufacturing a feed additive according to the present invention, as in the aforementioned organic matter, an antioxidant substance and a live bacterial agent are added, and the container is added to the environment, and the hair is made at a temperature of 25 ° C to 37 ° C. Therefore, in In advanced organics, lactic acid bacteria, yeast, acetic acid bacteria, and photosynthetic culture medium (individual or mixed form) prepared by the above-mentioned antioxidant substances are added as living bacteria. However, for a more suitable embodiment, the prepared lactic acid yeast culture liquid (if necessary, acetic acid can be added thereto and mixed, and the obtained mixed culture liquid is cultured with antioxidants and sugars to prepare a composite culture liquid. The culture solution of the compound culture bacteria is added to a high-grade organic substance to obtain a mixture, and a desired feed additive can be obtained by fermentation. In the method for producing a feed additive of the present invention, a pre-culture solution and a yeast culture solution (if necessary) The culture solution that is revived therein is mixed, and it is equal to the antioxidant and the carbohydrate, and the compound culture solution is prepared in the following embodiment. The body (with 2 kinds of W culture solution (cultivation) Wait for each photosynthetic dry bacterial solution (medium) method, add the bacterial culture medium and the culture medium of the bacteria in a high-closed ventilation ring), and reconstitute the solution and photosynthetic lactic acid bacteria in the mixture in the presence of 5 * Compound culture under various conditions with acetic acid bacteria L 557201 V. Description of the invention (7) (Lactobacillus acidophilus), Lactobacillus delbrueckii lactic acid subspecies (La ctobacillus delbruecki i subsp lactis), Lactobacillus brevis, or Lactobacillus buchneri belong to the bacterium; moreover, photosynthetic bacteria are Rhodopseudomonas pahstris, or spherical Red false = cell g (Rhodopse Uaomonas spher〇ides) belongs to the fine g is better than the saccharomyces cerevisiae with soap / yeast (SaCCharomyces cerevisiae) or effective gel = ^ nrdAida vaHda) is better. When using acetic acid bacteria, Better. ^ The bacteria belonging to dry Cet〇bacter liquefaciens) is: The feed additive obtained by ΚίίΪΪί is added to the feed nutrients for livestock, ΐίΪί, organic acids, vitamins, nucleic acids, etc., to achieve the healthy entry of feces and urine The growth of bacteria in f, remediation into a healthy intestinal environment, production of Ϊsaturation 1 Ϊ, the suppression of pathogenic bacteria, the suppression of foul odor, livestock [real: broadening and improving animal productivity. An example is the United States. This article illustrates the effect of fermented feed additives on livestock feed. The present invention, but the content of the present invention is cultivated. Taiga for livestock; material 'and add this feed

557201 V. Description of the invention (in gram, prepared by 1 liter of distilled water), medium of ρ Η 6 · 9 formed by 1 liter of distilled water, irradiated at 30 ° C with illumination 2 0 0 0 L u X fluorescent lamp Cultivate under anaerobic conditions to obtain 10V ml of culture solution. Milk, Sour Bacteria _, Yeast and Acetic Bacteria Fuhe Pei Juan Ji Xi _ f Add 3 parts by weight of the culture solution of the lactic acid bacteria, 3 parts by weight of the culture solution of the yeast, and 2 parts by weight of the culture solution of the acetic acid bacteria. The antioxidant content is 0.01% by weight, 10% by weight of iKMX liquid (product of Korea Colin Co., Ltd.) and 5 parts by weight of molasses as molasses. The lactic acid bacteria and yeast are obtained by compound culture in 27 艽. i 〇〇〇 / mL, pH 3.2 of the culture medium 0 — will be used as antioxidants 〇01% by weight of the "dilute solution 10 parts by weight (products of Korea Ke Co., Ltd.), lactic acid bacteria, 10 parts by weight of yeast and acetic acid bacteria, culture medium aa of photosynthetic bacteria, 5 weight wheat each: 5 weight 2: 87% by weight of rice bran of grade organic matter, 2% by weight of fish meal, wheat amount, 5% by weight of large denier meal, and oyster shell 1 Stir the contents and stir. # To the moisture content of 22 will be 2% of the corpse plastic bag formed, with technical private q 7. The mixture 'fill it into plastic repair & keep at 37 C, while Make it s > heat, produce fermented feed additives. The above-mentioned production method is referred to as "the present fermented feed additive"; the V feed ΛΛ feed additive (hereinafter referred to as the test. Laying hen breeding system is based on) laying hens fed with Zhaoyi Hungarian feed, and the breeding house is implemented. 1 Disinfection is performed in accordance with the general feeding tube; the method of 'chicken uu-ln all_out) is performed. Flail all in and out First, buy laying hens from a chicken farm. 3% by weight of this fermented feed is added to 557201 V. Description of the invention (11) The material is mixed evenly with the commercially available raw egg feed and is automatically fed to the processing area by the automatic feeding machine, and only the commercially available raw egg is administered. In the control area of the feed, the laying hens were separated and placed in egg cages. Each chicken was then given a 2 week preparatory period. _ Then, calculate the feed intake of chickens by measuring various feed residues in units of one week. Furthermore, the feed intake rate was calculated on the basis of roughly raw laying hens. The egg production rate during egg inspection is that the eggs are collected at 10 am every day, the egg production rate of the chickens in the treatment area and the control area is calculated according to the ratio, and the eggs are randomly selected daily from the eggs laid. Six, the egg weight, the yolk weight, the egg shell weight, etc. were measured by an electronic scale, and the length of the egg, the short diameter of the egg, and the thickness of the egg shell were measured using a calliper. Table 1 shows the productivity of the fermented feed additive ® added to laying hen feed. [Table 1] Change in the treatment area of the control area (%) Egg production rate (%) 80.00 83.00 3.75 Egg weight (g) 63.71 66.05 3.67 Egg length diameter (mm) 57.48 60.89 5.93 Egg short diameter (mm) 44.66 45.06 0.90 Egg shell thickness (Mm) 0.412 0.419 1.70 Eggshell weight (g) 7.27 7.55 3.85 Egg breakage% 1.80 1.40 22.22 If you follow Table 1, you can confirm the following characteristics. 1) Relative to 80% of the laying hens in the control area, the egg production rate in the treatment area is 83%, which is an increase of 3.75% compared with those in the control area. 2) Relative to the egg weight in the control area of 6 3 · 7 1 g, the treatment area is 6 6. 5 5 g

Page 14 557201 V. Description of the invention (12) grams, compared with those in the control area, an increase of 3.67%. In addition, an increase of 2.34 grams per egg 'is calculated to be a huge amount based on the total weight of the eggs. 3) Relative to the control area, the egg yolk weight was 6 · 33 grams, and the treatment area was ~ 16 · 08 ^ g, which was reduced by 5 · 3% compared with those in the control area. The only reason for this reduction is considered to be a decrease in the content of cholesterol in the egg yolk "" ^^^^. 4) Compared with the control area, the egg short diameter of the treatment area increased by 0.9%, and the egg length diameter was the same. The comparison of the control area showed that the egg length diameter of the treatment area increased by 5.93%. This kind of change in egg length contributes to an increase in overall egg production rate and an increase in egg weight (5 stomach). Compared with the control area, the thickness of the egg shell in the treatment area increases by 7%, and the egg to drum weight. Also, compared with those in the control area, the eggshell weight in the treatment area increased by 83%. The thickness of the egg shell is also the same, and the processing area is increased by 0.07 mm to make the egg M ′ to suppress egg breakage. The reason for the increase in egg shell weight is not only because of the increase in the thickness of the egg, but also because of the increase in the size of the egg. 1 6. ) Compared to the control area, the egg breaking rate was 8%, and the egg breaking rate in the treatment area was ^ 4%, which was 22 · lower than that in the control area. This phenomenon is due to the increase in egg shell weight and egg shell weight, which results in a significant decrease in egg breaking rate. _ 5 weeks to check the changes of Salmonella pathogenic bacteria. The same formula as in Example 1 was used to measure the changes in the excretion of pathogenic bacteria Salmonella typhimurium (Salmonel la typhimuiUm) in three groups from laying hens divided into the treatment area and the control area. The results are shown in Table 2. [Table 2] I. »--- (log 10 Salmonella / gram excrement) ms photo area ~ _________-_ ^ 2 3 5.26 soil 1.43 4.87 ± 0.98 6.22 ± 1.32 _ treatment area 114 ± 0.40 2.02 ± 0.72 2. 诏± 0.69

Page 15 557201 V. Description of the invention (13) Table 2 shows that the pathogenic bacteria Salmonella contained in the laying hens treated with the fermented feed supplement can reduce the pathogenic bacteria Salmonella by more than 50% compared with those in the control zone. Example 3 In the same manner as in Example 1, 15 laying hens divided into a treatment zone and a control zone were selected and divided into 3 groups. The fresh excreta were collected, and the number of bacteria and the frequency of occurrence were calculated after cultivation. The results are shown in Table 3 compared with those in the control area. [Table 3] 1 2 3 Control area 14/15 (93) 15/15 (100) 13/15 (87) Processing area 6/15 (40) 3/15 (20) 7/15 (47) Table 3 The test results of the presence of Salmonella in the listed fecal urine culture (Salmonella culture-number of positive chickens /% of total chickens) showed that the amount of Salmonella in the laying hens in the treatment area after culture was reduced compared with those in the control area To less than half. Example 4 Using ammonia to analyze the test reagent, in the same manner as in Example 1, the changes in ammonia content (micrograms / gram) in the urine of the three groups were investigated and compared for the laying hens divided into the treatment zone and the control zone. The results are shown in Table 4. [Table 4] 1 2 3 control area 20.31 ± 3.67 35.5 ± 6.21 28.01 ± 4.92 treatment area 5.32 ± 1.29 7.39 ± 2.80 12.64 ± 3.58

Page 16 557201 V. Description of the invention (14) Table 4 shows that compared with those in the control area, the ammonia content of the laying hens in the treatment area can be reduced to 55 to 79%. This change can be found to be even greater when the intestinal composition of laying hens is based. Example 5 Using a cholesterol test measurement set test analysis, in the same manner as in Example 1, the changes in the content of cholesterol in the yolk of the three groups were investigated for laying hens divided into the treatment zone and the control zone, respectively, and compared. The results are shown in Table 5. In addition, eggs were collected through a 90-day sample survey of the fermented feed supplement in the laying hen feed. [Table 5] 1 2 3 Control area 5.16 ± 2.20 6.30 ± 2.20 4.57 Soil 1.66 Treatment area 4.66 ± 1.19 5.54 ± 1.29 4.62 ± 1.68 Table 5 shows that the laying hens in the treatment area have an average cholesterol content compared to those in the control area. There is a tendency to decrease. Example 6 In the same manner as in Example 1, a breeding test was performed on a broiler chicken divided into a treatment zone and a control. Finally, θ 〇 was added to commercially available broiler feed, and the fermented feed supplement obtained in Example 1 was used to investigate the effect on the weight ratio. The breeding system advances in accordance with general breeding management guidelines, and the pheasant / chicken grows. In this example, broiler chickens (120, females of the same number) are from the age of 辉 °°, and after the end of the preparatory period, 5 stars from 3 days old. The broiler chickens that were transported for one day were fed with 3% by weight of the present fermented feed obtained in Example 1, and only commercially available broiler feed was administered to the broiler chickens in the treatment zone and the control zone. ‘'Additions, same as mother ’s week —

Page 17 557201 V. Explanation of the invention (15) Time (10 am) The growth and change of broilers in the treatment area and the control area were measured, and the values obtained by subtracting the weight at the beginning from the end weight are shown in Table 6. [Table 6] Control area Treatment area SEM 3 years old body weight (g) 60 63 5 1 week old body weight (g) 140 147 17 2 week old body weight (g) 405 397 40 3 week old body weight (g) 720 724 85 4 Body weight at week age (g) 1038 1026 117 Body weight at 5 weeks age (g) 1510 1525 169 Table 6 shows that the broilers in the treatment area grew normally, and at the age of 5 weeks they grew to _ about 1.5 kg. Example 7 Broilers separated into a treatment zone and a control zone were treated in the same manner as in Example 1, and the influence of microorganisms in the broiler farm was investigated. Broilers (120, females) in the treatment area and the control area were transported from the hatchery for 1 day of age. After the end of the preparatory period, 5 weeks from 3 days of age, 3 weights were given to the broilers in the treatment area. % The present fermented feed supplement obtained in Example 1, and only commercially available broiler feed was administered to broilers in the control area. After 4-week-old broiler chickens were slaughtered, the abdomen was cut open in a way that did not cause contamination, the small intestine and the cecum were cut open to a size of 1 cm, diluted with distilled water, and dissolved in agar medium. They were cultured in a microorganism incubator at a constant temperature of 36 ° C for 24 hours, and colony forming unit (cfu) was divided into 8 groups for measurement. The results of coliform changes in the intestine of 4-week-old broilers in the treatment area were compared with those in the control area, as shown in Table 7.

Page 18 557201 V. Description of the invention (16) [Table 7] 1 2 3 4 5 6 7 8 Cfu / Petri dish average 値 control area 3 2 7 4 9 2 2 4 33/8 4.13 Treatment area 1 1 3 2 1 4 3 2 17/8 2.13 Table 7 shows that compared with those in the control area, the chickens in the treatment area had an average reduction of 50% in coliform bacteria. This result is due to the action of microorganisms contained in the fermented feed supplement and the substances produced thereby activating useful intestinal bacteria and obtaining the effect. [Effects of the invention] It is obvious from the above that by feeding the feed # additive obtained by the manufacturing method of the present invention to livestock, the environment of bacteria in the intestine of the livestock can be improved, and the intestinal environment can be cured. To achieve the improvement of feces and urine, the suppression of pathogenic bacteria, the suppression of malodor and flatulence, the improvement of the quality of livestock products and the improvement of livestock productivity, etc., can contribute to the reduction of farmer operating costs.

Page 19 557201 Schematic description

Claims (1)

Buyou _ No .. £ 〇.] 10204 _ May 6, V. Application for patent scope 1. A method for manufacturing material additives, which is characterized by adding anti-oxidant substances and live bacteria agents in high-grade organic matter, and adding airtight In a container under aeration environment, it is fermented at a temperature of 25 to 37 ° C., wherein the viable bacterial agent is a viable bacterial agent containing at least one of lactic acid bacteria, yeast, and photosynthetic bacteria, and added with antioxidant substances and viable bacteria. The moisture content of the higher organics of the agent is in the range of wt%. 2 · If the feed additive manufacturing method in the scope of patent application No. 1 is used, the container in the closed and ventilated environment is a plastic bag. 3. If the lactic acid bacteria in the method of manufacturing a feed additive according to item 1 of the patent application scope are Lactobacillus paracasei (L act 〇bac 丨 1 1 us H paracase i subsp. Paracase i), Lactobacillus salivarius ^ (Lactobacillus sal ivaius), Lactobacillus acidophilus g (L act ob ac i 1 1 usaci doph i 1 us), Lactobacillus delbrueckii subsp lactis, Lactobacillus brevis or Lactobacillus uchneri; photosynthetic bacteria are Rhodopseudomonas palustris or Rhodopseudomonas spheroides; Saccharomyces cerevisiae or Saccharomyces cerevisiae Candida mortar amends this 25 and other? K § 隹 f 味 t 王 va 1 i da? Ben 4 · The method of manufacturing a feed additive according to any one of claims 1 to 3 of the patent application scope, wherein the live bacteria agent is further Contains acetic acid bacteria. 5. The method for producing a feed additive according to item 4 of the patent application scope, wherein the acetic acid bacteria is Acetobacter liquefaciens 0: \ 70 \ 70905-920430.ptc Page 21 557201 Amendment No. 90110204 Sixth, the scope of patent application belongs to bacteria. 6. A feed additive characterized by being manufactured by a manufacturing method according to any one of items 1 to 3 and 5 of the aforementioned patent application scope. 7. A feed additive characterized by being manufactured by a manufacturing method according to item 4 of the aforementioned patent application. 8. Feed for livestock, which is characterized in that it contains 0.4 to 3% by weight of a feed additive according to item 6 of the aforementioned patent application. Q: \ 70 \ 70905-920430.ptc Page 22