TW202128973A - Cleaning agent, and cleaning method and cleaning device using cleaning agent - Google Patents

Abstract

Provided are a cleaning agent having adequate cleaning power and a cleaning method and a cleaning device that use a cleaning agent. The cleaning agent according to one embodiment contains a fluorine-based alcohol as a compound that inactivates viruses. The cleaning agent according to one embodiment contains a fluorine-based alcohol as a compound that kills bacteria. In the cleaning agent according to one embodiment, the fluorine-based alcohol is represented by the general formula RfCH2OH or RfRf'CHOH; Rf and Rf' represent a C1-10 perfluoroalkyl group, and Rf and Rf' may be the same or different from each other.

Description

Cleaning agent, cleaning method and cleaning device using cleaning agent

This disclosure relates to a cleaning agent, a cleaning method and a cleaning device using the cleaning agent.

The society is highly concerned about infections that use viruses such as influenza virus or norovirus as pathogens, requiring a comfortable and clean living environment, and the demand for antiviral products is expanding in various fields. Especially in the medical field where cleanliness is required, infection countermeasures are very important, and it is helpful to protect the safety of patients and medical staff through reliable implementation. For example, medical equipment used by patients will be cleaned, disinfected, and sterilized according to the purpose of use of the equipment. These treatment methods are selected based on the risk of infection when using medical devices. Regardless of the method, adequate cleaning is the most important. As for why, imperfect cleaning will become the cause of imperfect disinfection and sterilization in the future.

The cleaning of medical equipment is roughly divided into three types: manual cleaning (including immersion cleaning), ultrasonic cleaning, and washer-sterilizer. The cleaning method is selected in consideration of the facility environment or the characteristics of medical equipment. The cleaning agents for medical equipment are roughly divided into three types: alkaline cleaning agents, acidic cleaning agents, and enzyme cleaning agents. Although the alkaline cleaning agent has excellent cleaning power, it has a strong effect on the material and the skin. Although acidic cleaning agents are suitable for cleaning inorganic substances (rust, scale, scale, oxide film, etc.), they are highly corrosive to metals. Although the enzyme-based cleaning agent has little effect on the material, its cleaning power is inferior. Therefore, it is necessary to choose an appropriate cleaning agent according to the characteristics of medical equipment or cleaning methods (mechanical cleaning, manual cleaning, immersion cleaning).

In various fields, cleaning is really important, and it is necessary to select the appropriate cleaning agent according to the target to be cleaned and the person to be removed from the target. For example, in the semiconductor manufacturing process, it is known to use a cleaning agent containing 1,1,1,3,3,3-hexafluoro-2-propanol. Patent Document 1 has disclosed that the polymer solubility of 1,1,1,3,3,3-hexafluoro-2-propanol is used to survive in STI, metal gates, contact holes, vias, capacitors, metal wiring, etc. The polymer stripping of the sidewall, and for the removal of photoresist residues after ion implantation or the stripping of attached polymer after dry etching in the single damascene or dual damascene process or after CMP in the single damascene or dual damascene process For cleaning, a cleaning composition containing 1,1,1,3,3,3-hexafluoro-2-propanol is used. Patent Document 2 has disclosed the property that 1,1,1,3,3,3-hexafluoro-2-propanol can be mixed with water in an arbitrary ratio and used in the cleaning and drying of semiconductor wafers in each lithography process1 ,1,1,3,3,3-hexafluoro-2-propanol vapor.

『Patent Literature』 "Patent Document 1": Japanese Patent Publication No. 2015-108041 "Patent Document 2": Japanese Patent Publication No. H3-106024

The purpose of this disclosure is to provide a cleaning agent with sufficient cleaning power, a cleaning method and a cleaning device using the cleaning agent.

The inventors of the present invention have studied intensively and found that 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and 2,2,2-trifluoroethanol (TFE) are fluorine-based alcohols. And 2,2,3,3,3-pentafluoro-1-propanol has virus deactivation and bactericidal effects.

This disclosure includes the following aspects.

[1] A cleaning agent containing a fluorine-based alcohol as a compound for deactivating viruses.

[2] A cleaning agent in which the fluorine alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent perfluoroalkyl groups with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[3] A cleaning agent, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[4] A cleaning agent in which the concentration of the fluoroalcohol when acting on the virus is 0.1% by mass or more.

[5] A cleaning agent, which further contains a solvent.

[6] A cleaning agent, wherein the virus is selected from at least one of the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[7] A cleaning agent used for cleaning medical appliances.

[8] A cleaning agent used for soil cleaning.

[9] A cleaning method comprising allowing a fluorine-based alcohol to act on a virus.

[10] A cleaning method in which the fluorine alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent perfluoroalkyl groups with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[11] A cleaning method, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[12] A cleaning method in which the concentration of the fluoroalcohol when acting on the virus is 0.1% by mass or more.

[13] A cleaning method in which the fluorine-based alcohol is diluted with a solvent.

[14] A cleaning method, wherein the virus is selected from at least one of the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[15] A cleaning method used in the cleaning of medical appliances.

[16] A cleaning method used in soil cleaning.

[17] A cleaning device comprising: a cleaning agent supply device that supplies a cleaning agent containing a fluorine-based alcohol as a compound that deactivates viruses.

[18] A cleaning device in which the fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[19] A cleaning device, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[20] A cleaning device in which the concentration of the fluoroalcohol when acting on the virus is 0.1% by mass or more.

[21] A cleaning device in which the fluorine-based alcohol is diluted with a solvent.

[22] A cleaning device, wherein the virus is selected from at least one of the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus.

[23] A cleaning device used for cleaning medical appliances.

[24] A cleaning device used for soil cleaning.

[25] A cleaning agent containing a fluorine-based alcohol as a compound that kills bacteria.

[26] A cleaning agent in which the fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent perfluoroalkyl groups with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[27] A cleaning agent, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[28] A cleaning agent in which the concentration of the fluorine-based alcohol when acting on bacteria is 0.1% by mass or more.

[29] A cleaning agent, which further contains a solvent.

[30] A cleaning agent used for cleaning medical appliances.

[31] A cleaning agent used for sterilization.

[32] A cleaning agent used to clean the soil.

[33] A cleaning method comprising causing a fluorine-based alcohol to act on bacteria.

[34] A cleaning method in which the fluorine alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent perfluoroalkyl groups with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[35] A cleaning method, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[36] A cleaning method in which the concentration of the fluoroalcohol when acting on bacteria is 0.1% by mass or more.

[37] A cleaning method in which the fluorine-based alcohol is diluted with a solvent.

[38] A cleaning method used in the cleaning of medical appliances.

[39] A cleaning method, which is used to clean the soil.

[40] A cleaning device comprising: a cleaning agent supply device that supplies a cleaning agent containing a fluorine-based alcohol as a compound that kills bacteria.

[41] A cleaning device in which the fluorine alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent perfluoroalkyl groups with 1 to 10 carbon atoms, and Rf and Rf' are different or the same as each other .

[42] A cleaning device, wherein the fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2,2,3, At least one of the group consisting of 3,3-pentafluoro-1-propanol.

[43] A cleaning device in which the concentration of the fluorine-based alcohol when acting on bacteria is 0.1% by mass or more.

[44] A cleaning device in which the fluorine-based alcohol is diluted with a solvent.

[45] A cleaning device used for cleaning medical appliances.

[46] A cleaning device used for soil cleaning.

According to one embodiment of the present disclosure, its purpose is to provide a cleaning agent with sufficient cleaning power, a cleaning method and a cleaning device using the cleaning agent.

[detergent]

The compound contained in the cleaning agent of the present disclosure is a fluorine-based alcohol. Fluorine-based alcohols are suitable as compounds for deactivating viruses.

In one embodiment, the cleaning agent contains a fluorine-based alcohol as an effective ingredient. In this specification, "cleaning" refers to removing contaminants attached to the target from the target. In addition, the so-called "pollutants" refer to, for example, blood or body fluids, fats, Prion Protein (PrP) and infectious amyloid-like proteins or organic matter such as cell tissues, microorganisms, viruses, and bacteria derived from living organisms. Wait. In one embodiment, the cleaning agent contains two or more kinds selected from fluorine-based alcohols.

In one embodiment, the fluorine-based alcohol disclosed in the present disclosure is represented by the following general formula (1) or (2). RfCH ₂ OH···(1) RfRf′CHOH···(2) In the general formula, Rf and Rf′ represent a perfluoroalkyl group having 1 to 10 carbon atoms.

Among the compounds represented by the general formula (1) or (2), particularly 1,1,1,3,3,3-hexafluoro-2-propanol (hereinafter sometimes referred to as HFIP), 2, 2,2-Trifluoroethanol (hereinafter sometimes referred to as TFE), 2,2,3,3,3-pentafluoro-1-propanol. Moreover, in one embodiment, the fluorine-based alcohol of the present disclosure includes 1,1,1-trifluoro-2-propanol (hereinafter sometimes referred to as TFIPL). Here, TFIPL can be racemate (hereinafter sometimes referred to as TFIPL (racemate)), S body (hereinafter sometimes referred to as S-TFIPL), R body (hereinafter sometimes referred to as R-TFIPL) Any aspect of it. In particular, HFIP and TFIPL are suitable as compounds for deactivating viruses.

In this specification, the so-called viruses, viruses that have a mantle (mantle virus) and viruses that do not have a mantle (no-mantle virus) are all referred to, and viruses that have DNA as their genome (DNA virus) And viruses that have RNA as their genome (RNA viruses) are all targets. In this specification, the term "virus" includes influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus, enterovirus, etc., but is not limited to these. Examples can be: viral hepatitis, viral meningitis, viral gastroenteritis, viral conjunctivitis, acquired immune insufficiency syndrome (AIDS), adult T-cell leukemia, Ebola hemorrhagic fever, yellow fever, cold syndrome, giant cell Viral infection, Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS), Progressive Multiple Leukemia, Varicella/Zoster, Herpes Simplex, Hand, Foot and Mouth Disease, Dengue Fever, Japanese Encephalitis, Infectious Erythema, Infectious mononucleosis, smallpox, rubella, acute polio (poliomyelitis), measles, pharyngeal conjunctival fever (pool fever), Marburg hemorrhagic fever, nephrotic hemorrhagic fever, Lyssa fever, epidemic parotid glands Viruses that are the pathogens of viral infections such as inflammatory disease, West Nile fever, herpes angina, and Qu Gong fever. In addition, it can be exemplified as an alternative virus to the virus mentioned above. Furthermore, as viruses that infect animals other than humans, there are rabies virus, hog cholera virus, and the like.

In one embodiment, the cleaning agent may also include the fluorine-based alcohol and solvent disclosed in the present disclosure. In one embodiment, the cleaning agent may also include a compound represented by the general formula (1) or (2) and a solvent. As the solvent capable of diluting the compound represented by the general formula (1) or (2), water, physiological saline, boric acid buffer, phosphate buffer (for example, phosphate buffered physiological saline (also called PBS)) , Acetic acid buffer, Tris buffer, HEPES buffer, methanol, ethanol, isopropanol, acetone, toluene, dimethyl sulfoxide, ethylene glycol, diethylene glycol and propylene glycol, etc. These may be one or more, but are not limited to these. In one embodiment, the cleaning agent includes a compound represented by the general formula (1) or (2) and the aforementioned solvent. HFIP is a colorless and transparent liquid with a melting point of -3.3°C and a boiling point of 58.6°C. TFE is a colorless and transparent liquid with a melting point of -45.0°C and a boiling point of 78.0°C. TFIPL is a colorless and transparent liquid with a melting point of -78°C and a boiling point of 22°C. HFIP, TFE and TFIPL are soluble in most solvents, so they can be adjusted to any concentration by diluting with solvent. In one embodiment, the content (mass%) of the solvent contained in the cleaning agent of the present disclosure may be 0 mass% or more and 99.9% by mass relative to the mass of the cleaning agent from the viewpoint of cleaning performance Below, 0 mass% or more and 99 mass% or less are preferable, 0 mass% or more and 92 mass% or less are particularly preferable, and 0 mass% or more and 71 mass% or less are more preferable.

In one embodiment, the cleaning agent may include additives in addition to the fluorine-based alcohol and solvent disclosed in the present disclosure. In one embodiment, the cleaning agent may include additives in addition to the compound represented by the general formula (1) or (2) and the solvent. Examples of additives that can be added to cleaning agents include: surfactants, enzymes, chelating agents, enzyme stabilizers, anticoagulants, anti-metal corrosion agents, low-molecular polyols, cleaning aids (detergents), and defoaming Agents, pH adjusters, fragrances, colorants, antioxidants, preservatives, bleaching agents, bleach activators, corrosion inhibitors, dispersants, thickeners, viscosity adjusters, etc., but are not limited to these. The cleaning agent may also contain one or more than two additives. In one embodiment, the cleaning agent can further improve the cleaning power by including the compound represented by the general formula (1) or (2), the solvent, and the above-mentioned additives.

Surfactants (A) include nonionic surfactants (A-1), anionic surfactants (A-2), cationic surfactants (A-3), and amphoteric surfactants (A-4) And biological surfactant (A-5).

Examples of nonionic surfactants (A-1) include: alkylene oxide addition type nonionic surfactants (A-1-1) and polyol type nonionic surfactants (A-1-2 )Wait.

Examples of alkylene oxide addition type nonionic surfactants (A-1-1) include: higher alcohols (8 to 18 carbons) alkylene oxides (2 to 4 carbons, preferably 2) adducts ( The number of added moles per active hydrogen is 1-30), alkyl (carbon number 1-12) phenol ethylene oxide (hereinafter sometimes referred to as EO) adduct (addition Mole number 1-30), higher amine (carbon number 8-22) alkylene oxide (carbon number 2-4, preferably 2) adducts (addition mole number per 1 active hydrogen is 1-40 ), fatty acid (carbon number 8-18) EO adduct (the number of moles added per 1 active hydrogen is 1-60), polypropylene glycol (molecular weight 200-4000) EO adduct (per 1 active hydrogen The number of addition moles is 1-50), polyethylene oxide (repeating unit number 3-30) alkyl (carbon number 6-20) aryl ether, and sorbitan monolaurate ester EO adduct (The number of added moles per active hydrogen is 1-30) and the EO adduct of sorbitan monooleate (the number of added moles per active hydrogen is 1-30), etc. (2 -8 valence or more) alcohol (carbon number 2-30) fatty acid (carbon number 8-24) ester EO adduct (addition mole per active hydrogen is 1-30), etc.

Examples of polyol type nonionic surfactants (A-1-2) include: glycerol monostearate, glycerol monooleate, sorbitan monolaurate and sorbitan monooleate Fatty acid (carbon number 8-24) esters of polyvalent (2-8 valent or more) alcohols (carbon number 2-30) such as alcohol anhydride esters, and fatty alkanolamines such as lauryl monoethanolamine and lauryl diethanolamine, etc. .

Examples of the anionic surfactant (A-2) include alkyl ether esters of carboxylic acid with 8 to 24 carbon atoms or their salts, and alkyl (poly)ethylene oxide ether esters of carboxylic acid with 8 to 24 carbon atoms. Or its salt (lauryl acetate (poly) ethylene oxide (polymerization degree = 1-100) ether ester sodium and sulfosuccinate lauryl (poly) ethylene oxide (polymerization degree = 1-100) ester disodium Etc.), sulfuric acid carbon number 8-24 alkyl ester salt and sulfuric acid carbon number 8-24 alkyl (poly) ethylene oxide ester salt Degree = 1～100) Sodium ester and lauryl sulfate (poly) ethylene oxide (polymerization degree = 1～100) ester triethanolamine salt, etc.), coconut oil sulphate amide monoethanol ester sodium, carbon number 8～24 Alkylbenzene sulfonate (sodium dodecylbenzene sulfonate, etc.), phosphate carbon number 8-24 alkyl ester salt, and phosphate carbon number 8-24 alkyl (poly)oxirane ester salt (lauryl phosphate) Sodium and phosphate lauryl (poly) ethylene oxide (polymerization degree = 1-100) ester ether sodium, etc.), fatty acid salt (sodium laurate and triethanolamine laurate, etc.), acylated amino acid salt (coconut oil syrup) Sodium methyl taurate, sodium coconut oil sarcosine sodium, coconut oil sarcosine triethanolamine, N-coconut oil glycine-L-glutamine triethanolamine, N-coco sarcosine- Sodium L-glutamate and sodium lauryl methyl-β-alanine, etc.).

Examples of cationic surfactants (A-3) include: quaternary ammonium salt type (stearyl trimethyl ammonium chloride, behenyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride) Base ammonium and ethyl sulfate lanolin fatty amine propyl ethyl dimethyl ammonium, etc.) and amine salt type (lactate diethyl amine ethyl stearyl amine salt, dilauryl amine hydrochloride salt and oleyl amine lactate Salt etc.) etc.

As amphoteric surfactants (A-4), betaine type amphoteric surfactants (coconut fat amine propyl dimethyl amino acetate betaine, lauryl dimethyl amino acetate betaine, 2- Alkyl-N-carboxymethyl-N-hydroxyethyl n-imidazole betaine, lauryl hydroxysultaine and lauryl amine ethyl hydroxyethyl carboxymethyl betaine hydroxypropyl sodium phosphate, etc.), amino group Acid type amphoteric surfactants (β-sodium lauryl propionate, etc.).

Examples of biosurfactants (A-5) include surfactins, rhamnolipids, and salts thereof. Examples of the salt include alkali metal salts, alkaline earth metal salts, and onium salts.

As the surfactant (A), one type or two or more types can be used. When two or more types are used, as the combination thereof, for example, nonionic surfactant (A-1), anionic surfactant (A-2), nonionic surfactant (A-1) ) Combinations with cationic surfactants (A-3), nonionic surfactants (A-1) and amphoteric surfactants (A-4), etc.

From the standpoint of cleaning properties, use a nonionic surfactant (A-1) alone or a combination of a nonionic surfactant (A-1) and an anionic surfactant (A-2) as the interface Active agent (A) is better.

As the nonionic surfactant (A-1), from the viewpoint of cleaning properties, an aliphatic alcohol (carbon number 8-24) ethylene oxide adduct (polymerization degree = 1-100) is preferred, It is better to use aliphatic alcohol (carbon number 12-18) ethylene oxide adduct (polymerization degree 4-20), and aliphatic alcohol (carbon number 12-15) ethylene oxide adduct (polymerization Degree=8-12) is more preferred, and 11 mol adduct of lauryl alcohol ethylene oxide is particularly preferred.

As an anionic surfactant (A-2), from the viewpoint of cleaning properties, alkylbenzene sulfonates with 8 to 24 carbon atoms, fatty acid salts, alkyl ester salts with 8 to 24 sulfuric acid carbons, and carbon sulfate Alkyl (poly)ethylene oxide ester salts with 8-24 are preferred, alkylbenzene sulfonates with 12-16 carbons and fatty acid salts with 8-16 carbons are more preferred, and dodecyl Benzenesulfonic acid monoethanolamine salt and sodium laurate are more preferred.

The content (mass %) of the surfactant (A) contained in the cleaning agent of the present disclosure is preferably 0 mass% or more and 10 mass% or less relative to the mass of the cleaning agent from the viewpoint of cleaning properties, It is more preferably 0.1% by mass or more and 5% by mass or less.

Enzyme (B) includes protease (B-1), amylase (B-2), lipase (B-3), cellulase (B-4), aminopeptidase, etc.

In this disclosure, the so-called protease (B-1) is an enzyme that catalyzes hydrolysis by using peptides or proteins as a substrate. As the protease (B-1), those derived from animals, plants or microorganisms are included. From the viewpoint of ease of acquisition, those derived from microorganisms are preferred. It also includes chemically or genetically modified variants. The protease (B-1) may also be a protease (alkaline protease) having the most suitable pH on the neutral to alkaline side, and multiple proteases satisfying this condition can be used in combination. As the protease (B-1), it includes serine protease (B-1-1), aspartic acid protease (B-1-2), cysteine protease (B-1-3) and metalloprotease ( B-1-4).

Serine protease (B-1-1) is a protease with serine residues as catalyst residues, including: chymotrypsin, trypsin, thrombin, cytoplasmin, elastase, subtilisin ( Subtilisin E, subtilisin BPN'), Kexin protease (Kexin), and protease derived from actinomycetes (Streptomyces), protease derived from Bacillus subtilis (Bacillus) or filamentous fungus (Koji) Bacteria) protease, etc. Specifically, examples include trypsin derived from porcine pancreas, subtilisin Novo derived from Bacillus, subtilisin Carlsberg, subtilisin 309, subtilisin 147, and subtilisin 168. Serine protease (B-1-1) is known to be a protease whose serine residue is involved in the active center. It will be specific to the diisopropyl fluorophosphate or benzyl sulfonate that binds to the serine residue. Fluorine and other agents lose their activity. Serine protease (B-1-1) does not require a reducing agent, is not affected by metal chelating agents, and has the most suitable pH for enzyme activity near neutral, so it is suitable for use in this disclosure.

Commercially available serine proteases (B-1-1) include: Alcalase, Savinase, Everlase, Esperase, Kannase, Ovozyme, Subtilisin A, PEM, PTN, Primase, Durazym, Long BIOPRASE manufactured by Seto Chemical Industry Co., Ltd., Protease N "Amano" and Protease P "Amano" manufactured by Amano Pharmaceutical Co., Ltd., Actinase AS manufactured by Scientific Research Pharmaceutical Co., Ltd., KAP manufactured by Kao Co., Ltd., Genencor Corporation Purafect, Purafect OXP, Properase, Pronase manufactured by Calbiochem, TrypLE Select manufactured by Invitrogen Corporation, Accutase manufactured by Chemicon International, etc. In addition, the protease described in Japanese Patent Publication No. 2007-61101 can also be suitably used.

Aspartic acid protease (B-1-2) is a protease with aspartic acid in the active center, including: pepsin, cathepsin D, cathepsin E, renin and chymosin, etc. Specifically, pepsin derived from the human stomach and the like can be cited. Aspartic acid protease (B-1-2) is a protease generally called acid protease, which has enzyme activity in the acidic region. Since HFIP is acidic, it is suitable.

Cysteine protease (B-1-3) is a protease whose thiol group exists in the active center, papain, bromelase, ficin, kiwifruit, cathepsin B, cathepsin H, cathepsin L, cysteine Winter enzymes and ginger protease, etc. Since the active center of cysteine protease (B-1-3) is a thiol group, it is better to use a reducing agent such as cysteine or thiourea in combination. From the viewpoint of preventing oxidation caused by oxygen in the air, such a reducing agent is preferably added to the cleaning agent immediately before or during cleaning.

Metalloprotease (B-1-4) is a protease containing metal ions in the active center, and examples thereof include thermolysin, matrix metalloprotease, carboxypeptidase A, carboxypeptidase B, dispase, and collagenase. Examples of commercially available metalloprotease (B-1-4) include dispase manufactured by Worthington Biochemical Corporation. In one embodiment, when the metalloprotease (B-1-4) is used in combination with the chelating agent (C) described later, from the viewpoint of maintaining the activity of the metalloprotease, a metal-free chelating agent is used as good.

Among the above-mentioned proteases (B-1), from the standpoint of durability and cleaning properties, serine protease (B-1-1) and aspartic acid protease (B-1-2) are preferred , Subtilisin and cytoplasmin are better. Among them, subtilisins derived from Bacillus halodurans and Bacillus clausii are preferred.

The cleaning agent of the present disclosure contains protease (B-1), which can more efficiently clean fixed protein stains. The protease (B-1) may be contained in the cleaning agent, but the cleaning agent containing the protease (B-1) can also be used in combination with the cleaning agent of the present disclosure. From the standpoint of enzyme stability, it is better to separately prepare a cleaning agent containing protease (B-1) and use it in combination immediately before or during cleaning.

As amylase (B-2), it includes those derived from bacteria or fungi. It also includes chemically or genetically modified variants. As an amylase (B-2), for example, the α-amylase obtained from a special strain of B. licheniformis described in detail in the specification of British Patent No. 1,296,839 can be cited. Commercially available amylases (B-2) include Duramyl, Termamyl, Fungamyl and BAN from Novozymes, and Rapidase and Maxamyl P from Gist-Brocades.

As lipase (B-3), those derived from bacteria or fungi are included. It also includes chemically or genetically modified variants. Examples of lipases include: Humicola lanuginosa lipase (European Patent No. 258068 and European Patent No. 30216), Rhizomucor miehei lipase, and Candida genus (Candida) lipase (European Patent specification No. 238 023), Candida antarctica (C. antarctica) A and B lipase, Pseudomonas (of Pseudomonas) lipase (European Patent specification No. 214 761), production class P. pseudoalcaligenes and P. alcaligenes lipase (European Patent No. 218272), P. cepacia lipase (European Patent No. 331376 Specification), P. stutzeri (P. stutzeri) lipase, P. fluorescens (P. fluorescens) lipase, and Bacillus ( Bacillus ) lipase (British Patent No. 1,372,034), subtilis B. subtilis lipase (Dartois et al. (1993), Biochemica et Biophysica Acta 1131, 253-260), B. stearothermophilus lipase (Japanese Patent Publication No. S64-744992), and Bacillus pumilus ( B. pumilus ) lipase (International Patent Publication No. 91/16422).

Commercially available lipases (B-3) include: M1 Lipase, Luma fast and Lipomax from Genencor, Lipolase and Lipolase Ultra from Novozymes, and Lipase P "Amano" from Amano Enzyme.

As cellulase (B-4), those derived from bacteria or fungi are included. It also includes chemically or genetically modified variants. As the cellulase, the cellulase disclosed in the specification of US Patent No. 4,435,307 is a fungal cellulase produced by Humicola insolens (Humicola insolens).

Examples of commercially available cellulase enzymes include Novozymes' Celluzyme produced by Humicola insolens strains and KAC-500(B) from Kao Co., Ltd.

Among the above-mentioned enzymes (B), protease (B-1) is preferred from the viewpoint of cleaning properties.

In the present disclosure, the enzyme (B) contained in the cleaning agent may include two or more kinds. As a combination containing two or more types, for example, a combination containing two or more types of protease, protease and amylase, protease and lipase, or protease and amylase and lipase.

The content (mass%) of the enzyme (B) contained in the cleaning agent of the present disclosure, from the viewpoint of cleaning properties, is preferably 0 mass% or more and 10 mass% or less with respect to the mass of the cleaning agent, and 0.05 Mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are particularly preferable.

As the chelating agent (C), any of amino acid, organic acid, phosphonic acid, phosphoric acid, and polycarboxylic acid can be used. Examples include: triacetoxyamine, iminodiacetic acid, ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid, ethylene glycol diamine ethyl ether tetraacetic acid, hydroxyethyl imine diacetic acid, Amino polyacetic acids such as ethylenetetramine hexaacetic acid and berytolic acid or their salts; glycolic acid, oxydisuccinic acid, carboxymethyloxysuccinic acid, citric acid, lactic acid, tartaric acid, oxalic acid, Malic acid, gluconic acid, carboxymethyl succinic acid, carboxymethyl tartaric acid, glutamine diacetic acid and other organic acids or their salts; amino tris (methylene phosphonic acid), 1-hydroxyethylene-1 , 1-Diphosphonic acid, ethylene diamine tetra (methylene phosphonic acid), ethylene triamine penta (methylene phosphonic acid) and other phosphonic acids or their salts; tripolyphosphoric acid and other phosphoric acids or their salts; polyacrylic acid , Polymethacrylic acid, polymaleic acid and other polycarboxylic acids or their salts. From the viewpoint of versatility, the chelating agent (C) is preferably one or more selected from aminopolyacetic acid and its salts, and preferably one selected from ethylenediaminetetraacetic acid (EDTA) and its salts One type or two or more types are preferable. Examples of counter ions for these salts include alkali metals, quaternary ammonium, alcohol amines, etc. However, in terms of corrosion resistance for medical devices, alcohol amine salts are preferred. Furthermore, monoethanolamine salt is preferred. These can be used individually by 1 type or in combination of 2 or more types.

The content (mass %) of the chelating agent (C) contained in the cleaning agent of the present disclosure is 0 mass% or more and 5 mass% from the viewpoint of the protein stain removal effect and cost Below, 0.005 mass% or more and 2 mass% or less are preferable, and 0.01 mass% or more and 1 mass% or less are more preferable. The content of the chelating agent (C) uses an acid-converted amount.

酸或此等的鹽等。本揭露之清洗劑中所包含之酵素穩定劑（D）的含量（質量%），就清洗性之觀點而言，以相對於清洗劑的質量為0質量%以上且10質量%以下為佳，以0.05質量%以上且5質量%以下為更佳，以0.1質量%以上且3質量%以下為尤佳。As the enzyme stabilizer (D), monosaccharides, polysaccharides, or boron compounds can be used. Monosaccharides and polysaccharides can be substituted or unsubstituted, and can also be branched or straight chain. Examples of monosaccharides and polysaccharides include dextrin, glucose, and mannose. The boron compound may be substituted or unsubstituted. Examples of boron compounds include boric acid, boron trioxide,

Acid or these salts, etc. The content (mass%) of the enzyme stabilizer (D) contained in the cleaning agent of the present disclosure, from the viewpoint of cleaning properties, is preferably 0 mass% or more and 10 mass% or less relative to the mass of the cleaning agent, It is more preferably 0.05% by mass or more and 5% by mass or less, and particularly preferably 0.1% by mass or more and 3% by mass or less.

Anticoagulant (E) includes sugar (E-1), non-surfactant (E-2), organic acid or its salt (E-3), inorganic oxo acid or its salt (E-4), Glycerol (E-5).

Examples of sugars (E-1) include allose, altose, mannose, gulose, idose, galactose, tarose, monosaccharides with 3 to 5 carbon atoms, and carbon number 6 ketoses, disaccharides or higher polysaccharides, sugar alcohols with 4 to 12 carbon atoms, etc. Examples of monosaccharides having 3 to 5 carbon atoms include glyceraldehyde, erythrulose, erythrose, ribose, xylose, and xylulose. Examples of the ketose having 6 carbon atoms include fructose, sorbose, and the like. Examples of polysaccharides higher than disaccharides include sucrose, lactose, cocoon sugar, cellobiose, sophorose, raffinose, maltotriose, carboxymethyl cellulose, starch, triglucose, pectin, Glucomannan and so on. Examples of sugar alcohols having 4 to 12 carbon atoms include sorbitol, xylitol, neopenteritol, maltitol, lactitol, sucralose, and the like.

Examples of non-surfactant (E-2) include: higher alcohol alkylene oxide adduct, alkyl (or alkenyl) phenol alkylene oxide adduct, styrenated phenol alkylene oxide adduct, benzene Ethylene alkylphenol alkylene oxide adduct, higher alkyl (or higher alkenyl) amine alkylene oxide adduct, fatty acid alkylene oxide adduct, fatty amine alkylene oxide adduct, polypropylene glycol ring Oxyalkylene adducts, (one or more) fatty acid glycerides or their alkylene oxide adducts, fatty acid sucrose esters or their alkylene oxide adducts, fatty acid sorbitan esters or their alkylene oxide adducts, etc.

Here, the higher alcohol is usually a linear or branched unsaturated or saturated higher alcohol with 8 to 24 carbons, and the alkyl or alkenyl phenol is usually a linear or branched alkyl or alkene with 6 to 22 carbons. Alkyl phenolic compounds, higher alkyl or higher alkenyl amines are usually linear or branched higher alkyl or higher alkenyl amines with 8-24 carbons, and fatty acids are usually unsaturated or saturated fatty acids with 8-24 carbons. , The weight average molecular weight of polypropylene glycol is 900-5000.

The alkylene oxide of the alkylene oxide adduct includes, for example, epoxy ethyl, epoxy propyl, epoxy butyl, and phenyl epoxy ethyl. These may be singly or in two or more types. . In the case of using two or more types, there is no restriction on the addition molding state of the alkylene oxide, and examples thereof include random addition, block addition, and a method of mixing a random and a block. The number of addition moles of the alkylene oxide adduct is 1 to 1,000.

Examples of organic acids or their salts (E-3) include amino acids, keto acids, oxycarboxylic acids, polycarboxylic acids, or saturated or unsaturated fatty acids having 1 to 24 carbon atoms, and salts thereof. Examples of organic acids include organic compounds having acidic groups such as carboxyl groups, sulfonic acid groups, phosphoric acid groups, thiol groups, and phenolic hydroxyl groups in the molecule. Examples of these salts include alkali metal salts and ammonium. Salt, alcohol amine salt, etc.

More specifically, examples of the organic compound having a carboxyl group include amino acids, keto acids, oxycarboxylic acids, polycarboxylic acids, saturated or unsaturated fatty acids having 1 to 24 carbon atoms, and the like. Examples of the organic compound having a sulfonic acid group include benzene sulfonic acid, linear alkylbenzene sulfonic acid, α-olefin sulfonic acid, and sulfuric acid monoester. Examples of the organic compound having a phosphate group include adenylic acid, atitic acid, hypophosphorous acid monoester, phosphate monoester, and phosphodiester. Examples of the organic compound having a thiol group include 4-mercaptoacetophenone, thiosalicylic acid, thiobenzyl acid, thioglycolic acid, and the like. As an organic compound which has a phenolic hydroxyl group, phenol, 2-naphthol, catechol, etc. are mentioned. As alkali metal salt, sodium salt, potassium salt, etc. are mentioned. Examples of the alcoholamine salt include monoethanolamine salt, diethanolamine salt, triethanolamine salt, and the like.

As an inorganic oxyacid or its salt (E-4), a phosphate, a hypophosphite, a pyrophosphate, a polyphosphate, a sulfate, etc. are mentioned. Examples of inorganic oxyacids include oxyacids such as phosphorus, sulfur, nitrogen, boron, chlorine, bromine, iodine, and silicon. Examples of these salts include alkali metal salts, ammonium salts, alcohol amine salts, etc. .

More specifically, examples of phosphorus-containing oxo acids include hypophosphorous acid, phosphorous acid, phosphoric acid, pyrophosphoric acid, and polyphosphates having a degree of polymerization of 3 to 6, and the like. Examples of sulfur oxyacids include hyposulfuric acid, sulfurous acid, sulfuric acid, persulfuric acid, pyrosulfuric acid, metabisulfuric acid, thiosulfuric acid, sulfamic acid, and disulfinic acid. As nitrogen oxyacid, nitrous acid, nitric acid, etc. are mentioned. Examples of the oxo acid of boron include metaboric acid, boric acid, perboric acid, and the like. Examples of chlorine-containing oxygen acids include hypochlorous acid, chlorous acid, chloric acid, perchloric acid, and the like. Examples of the oxygen-containing acid of bromine include hypobromous acid, bromous acid, bromic acid, perbromic acid, and the like. Examples of the oxo acid of iodine include hypoiodic acid, iodic acid, and periodic acid. Examples of the oxygen-containing acid of silicon include orthosilicic acid, metasilicic acid, and disilicic acid. Examples of alkali metal salts include sodium salts, potassium salts, and the like. Examples of alcoholamine salts include monoethanolamine salts, diethanolamine salts, and triethanolamine salts.

By containing the anticoagulant (E), the cleansing agent of the present disclosure can clean blood dirt more efficiently. For example, the content (mass %) of glycerol (E-5) contained in the cleaning agent of the present disclosure is relative to the quality of the cleaning agent in terms of the anticoagulant effect and the dissolving effect of coagulated blood It is 0% by mass or more and 80% by mass or less.

As the anti-metal corrosion agent (F), silicate can be used. For example, as the silicate, an alkali metal silicate can be cited. As the alkali metal silicate, a compound in which n of _{M 2} O· _{nSiO 2 is 0.3 to 5 can be used.} In addition, a suitable value of n is 1~3. From the viewpoint of light metal corrosion inhibition performance, n is preferably 0.3 or more, and from the viewpoint of preventing the generation of scale derived from silicic acid, n is preferably 5 or less. As the alkali metal silicate, for example, potassium orthosilicate, sodium orthosilicate, sodium sesquisilicate, potassium sesquisilicate, sodium metasilicate, potassium metasilicate, or 1 specified in JIS K1408 No. 2 sodium silicate, No. 2 sodium silicate, No. 3 sodium silicate, or the product name manufactured by Japan Chemical Industry Corporation: 1K potassium silicate, 2K potassium silicate, A potassium silicate (purity 40%) (mole ratio K ₂ O: SiO ₂ =1: 3) and so on. The content (mass%) of the anti-metal corrosion agent (F) contained in the cleaning agent of the present disclosure, from the viewpoint of corrosion inhibition performance, is preferably 0 mass% or more and 10 mass% or less relative to the mass of the cleaning agent .

The low-molecular-weight polyol (G) includes an alcohol compound having at least two hydroxyl groups and a carbon number of 2-30.

Examples of low-molecular polyols (G) include ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, butylene glycol, methyl propylene glycol, and pentane Alcohol, methyl butylene glycol, ethyl propylene glycol, dimethyl propylene glycol, hexylene glycol, methyl pentane glycol, ethyl methyl propylene glycol, dimethyl butylene glycol, heptanediol, propyl methyl propylene glycol, Isopropyl methyl propylene glycol, octanediol, ethyl hexanediol, secondary butyl propylene glycol, dimethyl hexanediol, trimethyl pentanediol, nonanediol, ethyl (2-methyl ) Propylene propylene glycol, trimethyl hexanediol, butyl ethyl propylene glycol, diethyl pentanediol, methyl octane diol, decane diol, dimethyl octane diol, undecane diol, ethyl Methyloctanediol, dodecanediol, diethyloctanediol, trimethylnonanediol, tetradecanediol, pentadecanediol, hexadecanediol, heptadecanediol, Saturated glycols such as octadecanediol, eicosanediol, behenyldiol, and tetracosanediol, or condensates thereof.

In addition, butenediol, methylene propylene glycol, butynediol, hexenediol, methylpentenediol, hexadienediol, octenediol, dimethylhexenediol , Decenediol, dimethylbutenediol, tetradecenediol, hydroxyoctadecenol, pentynediol, hexynediol, methylpentynediol, heptynediol, dimethyl Dimethylpentynediol, dimethylhexynediol, decynediol, dimethyloctynediol, tetramethyloctynediol, tetramethyldecynediol, tetramethyldodecynediol Unsaturated diols such as alcohol, tetraisopropyloctynediol, diethyltetradecynediol, etc.

In addition, examples include: cyclopentanediol, cyclohexanediol, cycloheptanediol, norethanediol, cyclooctanediol, cyclodecanediol, cyclooctenediol, decahydronaphthalenediol, and phenylene- Alicyclic diols such as 1,2-diol, terpene diol, dicyclohexanediol, cyclododecanediol, etc.

In addition, examples include glycerol, butanetriol, methylglycerol, pentanetriol, methylbutanetriol, trimethylolethane, hexanetriol, ethylbutanetriol, and trimethylol Trivalent alcohols such as propane, propylheptatriol, dimethyl glutamate, triethanolamine, and triisopropanolamine, etc.

In addition, examples include butane erythritol, neopentyl erythritol, pentaerythritol, hexaerythritol, diglycerol, sorbitol anhydride, N,N,N',N'-tetrakis (2-hydroxypropyl) ethyl Diamine, N,N,N',N'-tetra(hydroxyethyl)ethylenediamine and other tetravalent alcohols, etc.

In addition, examples include: pentaerythritol, arabitol, xylitol, triglycerol, and other pentahydric alcohols, as well as dineopentaerythritol, sorbitol, mannitol, iditol, inositol, and galactose Alcohol, tyrolose, allose and other hexavalent alcohols, etc.

In addition, examples include: methyl glycerol ether, ethyl glycerol ether, propyl glycerol ether, isopropyl glycerol ether, butyl glycerol ether, isobutyl glycerol ether, pentane Glycerol ether, hexyl glycerol ether, heptyl glycerol ether, octyl glycerol ether, (2-ethylhexyl) glycerol ether, nonyl glycerol ether, decyl glycerol ether Ether, undecyl glycerol ether, dodecyl glycerol ether, tridecyl glycerol ether, tetradecyl glycerol ether, hexadecyl glycerol ether, octadecyl glycerol ether, Eicosyl glycerol ether, allyl glycerol ether, undecenyl glycerol ether, octadecenyl glycerol ether, cyclohexyl glycerol ether, phenyl glycerol ether, etc. Alcohol-ethers and so on.

In addition, N-methyldiethanolamine, N-ethyldiethanolamine, N-propyldiethanolamine, N-isopropyldiethanolamine, N-butyldiethanolamine, N-cyclohexyldiethanolamine, N- (2-Ethylhexyl)diethanolamine and other N-substituted diethanolamines, etc.

In addition, N-methyldiisopropanolamine, N-ethyldiisopropanolamine, N-propyldiisopropanolamine, N-isopropyldiisopropanolamine, N-butyl N-substituted diisopropanolamines such as diisopropanolamine, N-cyclohexyldiisopropanolamine, N-(2-ethylhexyl)diisopropanolamine, etc.

In addition, N,N-disubstituted amino propylene glycol, such as dimethyl amino propylene glycol, diethyl amino propylene glycol, dipropyl amino propylene glycol, diisopropyl amino propylene glycol, and dibutyl amino propylene glycol, etc. kind. The low-molecular-weight polyols (G) in these examples must also contain positional isomer compounds.

The content (mass%) of the low-molecular polyol (G) contained in the cleaning agent of the present disclosure is preferably 0 mass% or more and 80 mass% or less from the viewpoint of cleaning performance relative to the mass of the cleaning agent .

Examples of cleaning aids (detergents) (H) include: polycarboxylates (acrylate homopolymers and maleate homopolymers, etc.), polycarboxylates (citric acid, malic acid, etc.) ) And alkaline detergents (caustic soda, sodium soda ash, ammonia, triethanolamine, sodium tripolyphosphate and sodium silicate, etc.). The content (mass %) of the detergent (H) contained in the cleaning agent of the present disclosure is preferably 0% by mass or more and 20% by mass or less relative to the mass of the cleaning agent from the viewpoint of cleaning properties.

Examples of the defoaming agent (I) include polysiloxane defoamers, polyalkylene oxide defoamers, mineral oil defoamers, and the like. The content (mass%) of the defoaming agent (I) contained in the cleaning agent of the present disclosure is preferably 0 mass% or more and 10 mass% or less from the viewpoint of cleaning properties relative to the mass of the cleaning agent.

Examples of the pH adjuster (J) include sulfuric acid, hydrochloric acid, citric acid, lactic acid, pyruvic acid, formic acid, sodium chloride, potassium chloride, monoethanolamine, and diethanolamine. The content (mass%) of the pH adjuster (J) contained in the cleaning agent of the present disclosure is preferably 0 mass% or more and 25 mass% or less relative to the mass of the cleaning agent from the viewpoint of cleaning properties, It is more preferably 0% by mass or more and 15% by mass or less, and particularly preferably 0% by mass or more and 10% by mass or less. In one embodiment, when the pH is on the alkaline side, the surface of the article to be cleaned after washing and drying may leave salt originating from the cleaning agent, and treatment such as wiping may be necessary in order to remove the salt. Therefore, depending on the cleaning target, the cleaning agent disclosed in the present disclosure may be preferably acidic, neutral, or weakly alkaline, and may be acidic or neutral. Specifically, the pH (25°C) of the cleaning agent of the present disclosure may be less than 9.0, and may be less than 7.0. In addition, the above content does not prevent the pH of the cleaning agent of the present disclosure from being used outside this range. Here, the above-mentioned pH (25°C) is the value measured by the method according to JIS Z 8802:2011 "pH Measurement Method".

The concentration of the fluorine-based alcohol when acting on the virus may be 0.1% by mass or more, preferably 1% by mass or more, and more preferably 8% by mass or more. The concentration of the fluorine-based alcohol when acting on the virus is preferably 29% by mass or more. In addition, the time for the fluorine-based alcohol to act on the virus is not particularly limited. The time for the fluorine-based alcohol to act on the virus is effective even for a short time. For example, the time for the fluorine-based alcohol to act on the virus may also be 30 seconds or more, preferably 1 minute or more, more preferably 20 minutes or more, and more preferably 30 minutes or more.

In one embodiment, the cleaning agent may also contain the effective ingredients of a commercially available disinfectant as an additive. As the effective ingredients of commercially available disinfectants, for example, the effective ingredients of high-level disinfectants (such as glutaraldehyde, ortho-phthalaldehyde, peracetic acid or peracetate), the effective ingredients of middle-level disinfectants (sodium hypochlorite) , Ethanol, Betadine), the active ingredients of low-level disinfectants (quaternary ammonium, loxidine gluconate). The content (mass%) of these active ingredients contained in the cleaning agent of the present disclosure may also be 0 mass% or more and 99.9 mass% or less with respect to the mass of the cleaning agent, with 0 mass% or more and 99 mass% or less as Preferably, it is more preferably 0% by mass or more and 92% by mass or less, and more preferably 0% by mass or more and 71% by mass or less.

The fluoroalcohol contained in the cleaning agent of the present disclosure, especially HFIP, is effective in deactivating viruses. In addition, HFIP can dissolve organic matter such as protein or cell tissue. Therefore, it becomes easier to remove organic matter such as protein or cell tissue attached to the cleaning target, and the cleaning power can be improved. HFIP is a stable low-molecular compound with a molecular weight of 168, with high storage properties. Moreover, the cleaning temperature is not limited due to its good thermal stability, which can further enhance the cleaning power. HFIP has low corrosiveness to metals and has little effect on the material of the cleaning target. Furthermore, HFIP is flame-retardant, and safety management in use is easy.

Moreover, TFIPL is effective for virus deactivation. In addition, TFIPL can dissolve organic matter such as protein or cell tissue. Therefore, it is easier to remove organic matter such as protein or cell tissue attached to the cleaning target, and the cleaning power can be improved. TFIPL is a stable low-molecular compound with a molecular weight of 114.07, with high storage properties. Moreover, the cleaning temperature is not limited due to its good thermal stability, which can further enhance the cleaning power. TFIPL has low corrosiveness to metals and has little effect on the material of the cleaning target. Furthermore, TFIPL is non-flammable, and safety management in use is easy.

[cleaning method]

The cleaning agent containing the fluorine-based alcohol of the present disclosure can be particularly used for cleaning medical appliances (including endoscopes, for example), and furthermore, can be used for target objects including animal medical appliances, edible meat processing appliances, and conditioning appliances. Clean. However, it is not limited to this, and can be widely used in virus infection countermeasures. For example, it can be used for the cleaning of operating rooms in the medical field and the cleaning of objects including linen fabrics such as beds and sheets, and disinfection of human hands.

The cleaning agent containing the fluorine-based alcohol of the present disclosure can also be used in, for example, manual cleaning (including immersion cleaning), ultrasonic cleaning, jet cleaning, shower cleaning, steam cleaning, vacuum cleaning, degassing cleaning, washer-disinfection Cleaning method, but not limited to these cleaning methods. For example, the cleaning method using a washer-disinfector generally consists of pre-cleaning, formal cleaning, washing, and disinfection processes. The cleaning agent containing the fluorine-based alcohol disclosed in the present disclosure can be used for preliminary cleaning and formal cleaning, and can also be used in combination with other cleaning agents for preliminary cleaning or formal cleaning. Here, the concentration of the fluorine-based alcohol contained in the permissible cleaning agent is the same as the concentration to deactivate the virus. Specifically, it may be 0.1% by mass or more, preferably 1% by mass or more, and 8% by mass or more. More preferably, 29% by mass or more is most preferable.

The cleaning agent containing the fluorine-based alcohol of the present disclosure can efficiently remove blood, body fluid, fat, and Prion Protein (Prion Protein , PrP) and infectious amyloid-like proteins or organic matter such as cell tissues, microorganisms, viruses, bacteria, etc.

However, the cleaning agent containing the fluorine-based alcohol of the present disclosure is not limited to the above-mentioned use, and can be compared with well-known antiviral agents, antibacterial agents, bactericides, disinfectants, and antifungal agents according to the cleaning target. And so on. For example, the method of spraying the cleaning target, the method of coating, the method of infiltrating the target, the method of immersing the target, the method of exposing the target to high-pressure steam, and other commonly used methods can be used for this.

The temperature at which the fluorine-based alcohol contained in the cleaning agent of the present disclosure deactivates the virus is not particularly limited, and a temperature above normal temperature (for example, above 20° C.) is preferred. If the temperature at which the fluorine-based alcohol comes into contact with the virus becomes higher, the virus becomes easier to deactivate. In addition, the temperature may be higher than the boiling point of the fluorine-based alcohol. In short, it may be vaporized into vapor and contact with the virus. In addition, the most suitable temperature can also be selected according to the solvent or additives contained in the cleaning agent.

[Cleaning device]

In one embodiment, a cleaning device including the fluorine-based alcohol function related to the present disclosure described above can be provided.

For example, the cleaning device 1 may also be used to clean medical appliances. As shown in FIG. 1, the cleaning device 1 includes a cleaning tank 10 having a storage portion 20 for accommodating a target object (medical instrument), and a cleaning agent supply device 40 that supplies cleaning agent into the cleaning tank 10. The washing tank 10 includes a water storage unit 12 that stores washing water under the storage unit 20, and water supply as washing water supply means for supplying water and hot water sent from a water supply source and a hot water source to the water storage unit 12 as washing water. The pipe 14 and the hot water supply pipe 16, the cleaning nozzle 22 that sprays the cleaning water of the water storage part 12 to the target contained in the cleaning tank 10, and the cleaning pump 18 that sends the cleaning water of the water storage part 12 to the cleaning nozzle 22.

In the cleaning method using the cleaning device 1, the medical instrument is cleaned by the preliminary cleaning process, the formal cleaning process, and the washing cleaning process, followed by boiling water disinfection in the disinfection process. The cleaning agent containing the fluorine-based alcohol disclosed in the present disclosure can be used for preliminary cleaning and formal cleaning, and can also be used in combination with other cleaning agents for preliminary cleaning or formal cleaning. In addition, in the case where the cleaning agent containing the fluorine-based alcohol of the present disclosure contains multiple components, a part or each of the components can also be used for preliminary cleaning or formal cleaning from a separate production line.

Once the cleaning process is started, the cleaning device 1 first performs a preliminary cleaning process. The cleaning device 1 opens the water supply valve, and starts the water supply process in the preliminary cleaning process. Thereby, the water of the water supply source is sent out to the water storage part 12 of the washing tank 10 through the water supply pipe 14. The cleaning device 1 determines whether or not the water in the water storage part 12 reaches the designated water level by the detection of the float switch. If the cleaning device 1 determines that the water in the water storage unit 12 has reached the specified water level, it closes the water supply valve and ends the water supply process. The water in the preliminary cleaning process may also be at room temperature (for example, 20°C).

The cleaning device 1 supplies the cleaning agent into the cleaning tank 10 by the cleaning agent supply device 40, and operates the cleaning pump 18 for a predetermined time as a preliminary cleaning process. Thereby, the washing water medium circulation pipe in the water storage part 12 is sent to the washing nozzle 22, and the washing nozzle 22 is rotated while spraying washing water to the medical instrument for washing. The washing water containing the washing agent in the washing tank 10 falls and flows back to the water storage part 12. After the preliminary cleaning process, the cleaning device 1 activates the drainage pump for a specified time as the drainage process. Thereby, the washing water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

Once the preliminary cleaning process is over, the cleaning device 1 will perform the formal cleaning process. In the main cleaning process, the cleaning device 1 opens the water supply valve and the hot water supply valve, and starts the water supply process in the main cleaning process. Thereby, the water from the water supply source and the hot water from the hot water source are sent out to the water storage unit 12 of the washing tank 10 through the water supply pipe 14 and the hot water supply pipe 16. The cleaning device 1 determines whether or not the water in the water storage part 12 reaches the designated water level by the detection of the float switch. If the cleaning device 1 determines that the water in the water storage unit 12 has reached the specified water level, it will close the water supply valve and the hot water supply valve to end the water supply process. The water in the main cleaning process is preferably 25°C or higher.

The cleaning device 1 supplies the cleaning agent into the cleaning tank 10 by the cleaning agent supply device 40, and operates the cleaning pump 18 for a predetermined time as the main cleaning process. Thereby, the washing water medium circulation pipe in the water storage part 12 is sent to the washing nozzle 22, and the washing nozzle 22 is rotated while spraying washing water to the medical instrument for washing. The washing water containing the washing agent in the washing tank 10 falls and flows back to the water storage part 12. After the formal cleaning process, the cleaning device 1 activates the drainage pump for a specified time as the drainage process. Thereby, the washing water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

The formal cleaning process can also be carried out several times. In this case, for example, it is also possible to use the cleaning agent separately and perform several formal cleaning treatments. In the case of using the cleaning agent containing the fluorine-based alcohol of the present disclosure in the first main cleaning process, the main cleaning treatment can also be performed with cleaning water at 60° C. or higher. In the case of using an enzyme-based cleaning agent in the second main cleaning process, it is also possible to perform main cleaning with cleaning water at approximately 30-40°C (for example, 37°C).

Once the formal cleaning process is over, the cleaning device 1 will perform the cleaning process. In the washing and washing step, the washing device 1 opens the hot water supply valve to start the hot water supply treatment in the washing step. Thereby, the hot water of the hot water supply source is sent out to the water storage part 12 of the washing tank 10 through the hot water supply pipe 16. The cleaning device 1 determines whether or not the hot water of the water storage part 12 reaches the designated water level by the detection of the float switch. If the cleaning device 1 determines that the hot water of the water storage unit 12 has reached the designated water level, it closes the hot water valve to end the hot water supply process.

The cleaning device 1 activates the cleaning pump 18 for a predetermined time as a cleaning process. Thereby, the hot water intermediary circulation pipe in the water storage unit 12 is sent to the cleaning nozzle 22, and the cleaning nozzle 22 rotates while spraying hot water to the medical instrument to flush the cleaning water containing the cleaning agent. After the washing treatment, the washing device 1 activates the drain pump for a predetermined time as the drain treatment. Thereby, the washing water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

Once the washing process is over, the cleaning device 1 will perform the disinfection process. In the disinfection process, the cleaning device 1 opens the hot water supply valve and starts the hot water supply process in the disinfection process. Thereby, the boiling water of the hot water supply source is sent out to the water storage part 12 of the washing tank 10 through the hot water supply pipe 16. The cleaning device 1 determines whether the boiling water of the water storage part 12 reaches the designated water level by detecting the float switch. If the cleaning device 1 determines that the boiling water of the water storage unit 12 has reached the specified water level, it will close the hot water valve to end the hot water supply process. The boiling water in the disinfection process is preferably above 60°C.

The cleaning device 1 activates the cleaning pump 18 for a specified time as a disinfection process. Thereby, the boiling water medium circulation pipe in the water storage part 12 is sent to the cleaning nozzle 22, and the cleaning nozzle 22 is rotated, and the boiling water is sprayed to the medical instrument. After the disinfection treatment, the cleaning device 1 activates the drain pump for a specified time as drain treatment. Thereby, the boiling water in the water storage unit 12 is discharged to the outside of the machine through the drain pipe 34.

The cleaning device 1 uses the cleaning agent supply device 40 to supply the cleaning agent containing the fluorine-based alcohol of the present disclosure into the cleaning tank 10, and can efficiently remove the blood, body fluid, fat, and prion protein derived from the target from the target. (Prion Protein, PrP) and infectious amyloid-like proteins or organic matter such as cell tissues, microorganisms, viruses, bacteria, etc. The cleaning device 1 may also use a cleaning agent containing the fluorine-based alcohol of the present disclosure and a well-known cleaning agent in combination.

［Soil cleaning agent］

The cleaning agent related to the present invention described above can be used as a soil cleaning agent in one embodiment. By using the soil cleaning agent containing the fluorine-based alcohol of the present disclosure, the deactivation of the virus contained in the soil can be expected. In one embodiment, the soil cleaning agent may also contain general additives described as well-known technologies. These also include pesticides, fertilizers, fungicides, and disinfectants used in the soil.

In one embodiment, the soil cleaning agent may also include the fluorine-based alcohol and solvent disclosed in the present disclosure. The solvent may be selected from substances capable of diluting the fluorine-based alcohol, and examples thereof include the same solvents as those already listed in the aforementioned cleaning agent. These may be one or more, but are not limited to these.

In one embodiment, the content (mass%) of the solvent contained in the soil cleaning agent may be 0 mass% or more and 99.9 mass% or less relative to the mass of the soil cleaning agent from the viewpoint of cleaning performance , Preferably 0 mass% or more and 99 mass% or less, more preferably 0 mass% or more and 92 mass% or less, and more preferably 0 mass% or more and 71 mass% or less.

In one embodiment, the soil cleaning agent may also include additives in addition to the fluorine-based alcohol and the solvent disclosed in the present disclosure. Examples of additives that can be added to soil cleaning agents include: surfactants, enzymes, enzyme stabilizers, anti-metal corrosion agents, low-molecular polyols, cleaning aids (detergents), defoamers, pH adjusters, fragrances , Colorants, antioxidants, preservatives, bleaching agents, bleach activators, corrosion inhibitors, dispersants, thickeners, viscosity modifiers, etc., but not limited to these. The soil cleaning agent may also contain one or more than two additives. In one embodiment, the soil cleaning agent can further improve the cleaning power by including fluorine-based alcohol, solvent and the above additives.

As the surfactant (A), for example, the same surfactants (A) listed in the aforementioned cleaning agent can be mentioned. As the surfactant (A), one type or two or more types can be used.

The content (mass%) of the surfactant (A) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is preferably 0 mass% or more and 10 mass% or less relative to the mass of the soil cleaning agent. It is more preferably 0.1% by mass or more and 5% by mass or less.

The enzyme (B) can be, for example, the same as the enzyme (B) listed in the aforementioned cleaning agent. As the enzyme (B), one kind or two or more kinds can be used.

In one embodiment, the content (mass%) of the enzyme (B) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10 mass% relative to the mass of the soil cleaning agent The following is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are especially preferable.

As the enzyme stabilizer (C), for example, the same enzyme stabilizer (D) listed in the aforementioned cleaning agent can be cited. As the enzyme stabilizer (C), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the enzyme stabilizer (C) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10% relative to the mass of the soil cleaning agent. Mass% or less is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are particularly preferable.

As the anti-metal corrosion agent (D), for example, the same ones as the anti-metal corrosion agent (F) listed in the aforementioned cleaning agent can be cited. As the anti-metal corrosion agent (D), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the anti-metal corrosion agent (D) contained in the soil cleaning agent is 0 mass% or more from the viewpoint of corrosion inhibition performance relative to the mass of the soil cleaning agent. 10% by mass or less is preferable.

Examples of the low-molecular-weight polyol (E) are the same as the low-molecular-weight polyol (G) listed in the aforementioned cleaning agent. As the low-molecular-weight polyol (E), one type or two or more types can be used.

In one embodiment, the content (mass %) of the low-molecular polyol (E) contained in the soil cleaning agent is 0 mass% or more from the viewpoint of cleaning performance relative to the mass of the soil cleaning agent. 80% by mass or less is preferable.

As the cleaning aid (washing agent) (F), for example, the same washing aids (washing agent) (H) listed in the above-mentioned washing agent can be mentioned. As the detergent (F), one type or two or more types can be used. In one embodiment, the content (mass %) of the detergent (F) contained in the soil cleaning agent is 0 mass% or more and 20% from the viewpoint of cleaning performance relative to the mass of the soil cleaning agent. The mass% or less is better.

As the defoaming agent (G), for example, the same as the defoaming agent (I) listed in the aforementioned cleaning agent can be cited. As the defoaming agent (G), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the defoaming agent (G) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10% relative to the mass of the soil cleaning agent. The mass% or less is better.

As the pH adjuster (H), for example, the same as the pH adjuster (J) listed in the aforementioned cleaning agent can be mentioned. As the pH adjuster (H), one type or two or more types can be used. In one embodiment, the content (mass %) of the pH adjuster (H) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 25% relative to the mass of the soil cleaning agent. Mass% or less is preferable, 0 mass% or more and 15 mass% or less are more preferable, and 0 mass% or more and 10 mass% or less are particularly preferable.

The concentration of the fluorine-based alcohol when acting on the virus may be 0.1% by mass or more, preferably 1% by mass or more, and more preferably 8% by mass or more. The concentration of the fluorine-based alcohol when acting on the virus is preferably 29% by mass or more. In addition, the time for the fluorine-based alcohol to act on the virus is not particularly limited. The time for the fluorine-based alcohol to act on the virus is effective even for a short time. For example, the time for the fluorine-based alcohol to act on the virus may also be 30 seconds or more, preferably 1 minute or more, more preferably 20 minutes or more, and more preferably 30 minutes or more. In order to improve the effect, it is also better to use the method several times in segments with the interval of daily unit or weekly unit or even each season opened.

In one embodiment, the soil cleaning agent may also contain the effective ingredients of a commercially available disinfectant as an additive. As the effective ingredient of the commercially available disinfectant, for example, the same effective ingredients as those of the commercially available disinfectant listed in the aforementioned cleaning agent can be cited. In one embodiment, the content (mass%) of these active ingredients contained in the soil cleaning agent may also be 0 mass% or more and 99.9 mass% or less relative to the mass of the soil cleaning agent, with 0 mass% The above is preferably 99% by mass or less, more preferably 0% by mass or more and 92% by mass or less, and more preferably 0% by mass or more and 71% by mass or less.

In one embodiment, the fluorine-based alcohols contained in the soil cleaning agent, especially HFIP and TFIPL, are effective in cleaning soil contaminated by viruses. HFIP and TFIPL are stable low-molecular compounds with high storage properties. Moreover, the cleaning temperature is not limited due to its good thermal stability, which can further enhance the cleaning power. Furthermore, HFIP and TFIPL are non-flammable, and safety management in use is easy.

[cleaning method]

Use the soil cleaning agent related to this implementation type to clean soil contaminated by viruses or soil that may be contaminated. Alternatively, the soil cleaning agent related to this implementation type can also be used preventively to clean the soil. In one embodiment, the soil cleaning agent can also be diluted with the above solvent for cleaning. In one embodiment, by adding and mixing the soil cleaning agent or solvent and soil cleaning agent related to this embodiment to the soil of the cleaning target to separate and dry the cleaning soil, the virus can be deactivated for cleaning soil.

In one embodiment, the concentration of the fluorine-based alcohol or the compound represented by the general formula (1) during cleaning may also be 0.01% by mass or more, preferably 0.1% by weight or more, and particularly preferably 1% by mass or more. The degree of soil pollution at the time of use, the period of pollution, and the surrounding environment of the soil can be adjusted arbitrarily.

In one embodiment, the fluorine-based alcohol or the compound represented by the general formula (1) can also be used as a soil fumigant. For example, by fumigating the suspected polluted soil with a soil fumigant containing a fluorine-based alcohol or a compound represented by the general formula (1), the soil can be cleaned.

[Bactericidal cleaning agent]

The cleaning agent related to the present invention described above can be used as a sterilizing cleaning agent in one embodiment. The fluorine-based alcohol disclosed in the present disclosure is suitable as a compound for sterilization. In particular, HFIP and TFE are suitable as compounds for sterilization. By using the bactericidal cleaning agent containing the fluorine-based alcohol of the present disclosure, the effect of killing bacteria can be expected.

In this specification, examples of bacteria include: Staphylococcus aureus, Escherichia coli, Enterohemorrhagic Escherichia coli, Tuberculosis, MRSA, Vancomycin-resistant Enterococcus, Multidrug-resistant Pseudomonas aeruginosa, Multidrug-resistant Acinetobacter Etc., but not limited to those.

In one embodiment, the bactericidal cleaning agent may also include the fluorine-based alcohol and solvent disclosed in the present disclosure. The solvent may be selected from substances capable of diluting the fluorine-based alcohol, and examples thereof include the same solvents as those already listed in the aforementioned cleaning agent. These may be one or more, but are not limited to these.

In one embodiment, the content (mass%) of the solvent contained in the disinfectant cleaning agent may be 0 mass% or more and 99.9 mass% or less relative to the mass of the cleaning agent from the viewpoint of cleaning properties. It is preferably 0% by mass or more and 99% by mass or less, particularly preferably 0% by mass or more and 92% by mass or less, and more preferably 0% by mass or more and 71% by mass or less.

In one embodiment, the bactericidal cleaning agent may also include additives in addition to the fluorine-based alcohol and solvent disclosed in the present disclosure. Examples of additives that can be added to the sterilization cleaning agent include: surfactants, enzymes, chelating agents, enzyme stabilizers, anticoagulants, anti-metal corrosion agents, low-molecular polyols, cleaning aids (detergents), and detergents. Foaming agents, pH adjusters, fragrances, colorants, antioxidants, preservatives, bleaching agents, bleach activators, corrosion inhibitors, dispersants, thickeners, viscosity adjusters, etc., but are not limited to these. The bactericidal cleaning agent may also contain one or more than two additives. In one embodiment, the bactericidal cleaning agent contains fluorine-based alcohols, solvents and the above additives to further enhance the cleaning power.

The surfactant (A) can be, for example, the same as the surfactant (A) listed in the aforementioned cleaning agent. As the surfactant (A), one type or two or more types can be used.

The content (mass%) of the surfactant (A) contained in the disinfectant cleaning agent, from the viewpoint of cleaning properties, is preferably 0 mass% or more and 10 mass% or less relative to the mass of the disinfection cleaning agent, 0.1 More preferably, it is greater than or equal to 5% by mass and less than or equal to 5% by mass.

The enzyme (B) can be, for example, the same as the enzyme (B) listed in the aforementioned cleaning agent. As the enzyme (B), one kind or two or more kinds can be used.

In one embodiment, the content (mass%) of the enzyme (B) contained in the disinfectant cleaning agent is 0 mass% or more and 10 mass% from the viewpoint of cleaning performance relative to the mass of the disinfectant cleaning agent The following is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are especially preferable.

As the chelating agent (C), for example, the same chelating agent (C) as mentioned in the aforementioned cleaning agent can be mentioned. As the chelating agent (C), one type can be used alone or two or more types can be used. In one embodiment, the content (mass %) of the chelating agent (C) contained in the antiseptic cleaning agent is 0 mass relative to the quality of the antiseptic cleaning agent in terms of the removal effect and cost of protein stains % Or more and 5 mass% or less, preferably 0.005 mass% or more and 2 mass% or less, and more preferably 0.01 mass% or more and 1 mass% or less. The content of the chelating agent (C) uses an acid-converted amount.

As the enzyme stabilizer (D), for example, the same enzyme stabilizer (D) listed in the aforementioned cleaning agent can be cited. As the enzyme stabilizer (D), one type can be used alone or two or more types can be used. In one embodiment, the content (mass %) of the enzyme stabilizer (D) contained in the disinfectant cleaning agent, from the viewpoint of cleaning performance, is 0 mass% or more and 10% relative to the mass of the disinfectant cleaning agent. Mass% or less is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are particularly preferable.

As the anticoagulant (E), for example, the same anticoagulant (E) as the anticoagulant (E) listed in the aforementioned cleaning agent can be cited. As the anticoagulant (E), one type may be used alone or two or more types may be used. For example, in one embodiment, the content (mass %) of glycerol (E-5) contained in the antiseptic cleaning agent is relatively The mass of the disinfectant cleaning agent is 0% by mass or more and 80% by mass or less.

As the anti-metal corrosion agent (F), for example, the same ones as the anti-metal corrosion agent (F) listed in the aforementioned cleaning agent can be cited. As the anti-metal corrosion agent (F), one kind or two or more kinds can be used. In one embodiment, the content (% by mass) of the anti-corrosive agent (F) is preferably 0% by mass or more and 10% by mass or less relative to the mass of the sterilizing cleaning agent from the viewpoint of corrosion inhibition performance.

The low-molecular-weight polyol (G) includes, for example, the same low-molecular-weight polyol (G) as the aforementioned cleaning agent. As the low-molecular-weight polyol (G), one type or two or more types can be used.

In one embodiment, the content (mass %) of the low-molecular polyol (G) contained in the sterilizing cleaning agent is 0% by mass or more from the viewpoint of cleaning performance relative to the mass of the sterilizing cleaning agent. 80% by mass or less is preferable.

As the cleaning aid (washing agent) (H), for example, the same washing aids (washing agent) (H) listed in the aforementioned washing agent can be mentioned. As the detergent (H), one type or two or more types can be used. In one embodiment, the content (mass%) of the detergent (H) contained in the disinfectant cleaning agent is 0 mass% or more and 20% from the viewpoint of cleaning performance relative to the mass of the disinfectant cleaning agent. The mass% or less is better.

As the defoaming agent (I), for example, the same as the defoaming agent (I) listed in the aforementioned cleaning agent can be cited. As the defoamer (I), one kind or two or more kinds can be used. In one embodiment, the content (mass%) of the defoaming agent (I) contained in the sterilizing cleaning agent is set to be 0% by mass or more and 10% from the viewpoint of cleaning performance relative to the mass of the sterilizing cleaning agent. The mass% or less is better.

As a pH adjuster (J), the same thing as the pH adjuster (J) mentioned in the said cleaning agent is mentioned, for example. As the pH adjuster (J), one type or two or more types can be used. In one embodiment, the content (mass%) of the pH adjuster (J) contained in the sterilizing cleaning agent is set to be 0% by mass or more and 25% from the viewpoint of cleaning performance relative to the mass of the sterilizing cleaning agent. Mass% or less is preferable, 0 mass% or more and 15 mass% or less are more preferable, and 0 mass% or more and 10 mass% or less are particularly preferable. In one embodiment, when the pH is on the alkaline side, the surface of the article to be cleaned after washing and drying may leave salt originating from the cleaning agent, and treatment such as wiping may be necessary in order to remove the salt. Therefore, depending on the cleaning target, the disinfectant cleaning agent disclosed in the present disclosure may be preferably acidic, neutral, or weakly alkaline, and may be acidic or neutral. Specifically, the pH (25°C) of the disinfectant cleaning agent disclosed in the present disclosure may be less than 9.0, and may be less than 7.0. In addition, the above content does not prevent the pH of the disinfectant cleaning agent of the present disclosure from being used outside this range. Here, the above-mentioned pH (25°C) is the value measured by the method according to JIS Z 8802:2011 "pH Measurement Method".

The concentration of the fluorine-based alcohol when acting on bacteria may be 0.1% by mass or more, preferably 1% by mass or more, more preferably 8% by mass or more, and particularly preferably 29% by mass or more. In addition, the time for the fluorine-based alcohol to act on the bacteria is not particularly limited. It is effective even for a short period of time for the fluorine-based alcohol to act on the bacteria. For example, the time for the fluorine-based alcohol to act on the bacteria may also be 30 seconds or more, preferably 1 minute or more, more preferably 20 minutes or more, and more preferably 30 minutes or more.

In one embodiment, the antiseptic cleaning agent may also contain the effective ingredients of a commercially available disinfectant as an additive. As the effective ingredient of the commercially available disinfectant, for example, the same effective ingredients as those of the commercially available disinfectant listed in the aforementioned cleaning agent can be cited. In one embodiment, the content (mass%) of these active ingredients contained in the disinfectant cleaning agent may also be 0 mass% or more and 99.9 mass% or less relative to the mass of the disinfection cleaning agent, with 0 mass% The above is preferably 99% by mass or less, more preferably 0% by mass or more and 92% by mass or less, and more preferably 0% by mass or more and 71% by mass or less.

In one embodiment, the fluorine-based alcohol contained in the disinfectant cleaning agent can also be used as a disinfectant. Among fluorine alcohols, HFIP and TFE are particularly suitable. These fluorine-based alcohols can show effective bacteria, for example: Staphylococcus aureus, Escherichia coli, Enterohemorrhagic Escherichia coli, Tuberculosis, MRSA, Vancomycin-resistant Enterococcus, Multidrug-resistant Pseudomonas aeruginosa , Multi-drug resistant Acinetobacter, etc., but not limited to these.

[cleaning method]

The bactericidal cleaning agent containing the fluorine-based alcohol disclosed in the present disclosure can be particularly used for cleaning medical appliances (including endoscopes, for example), and furthermore, can be used for target objects including animal medical appliances, edible meat processing appliances, and conditioning appliances Of cleaning. However, it is not limited to this, and can be widely applied to countermeasures against bacterial infections. For example, it can be used for the cleaning of operating rooms in the medical field and the cleaning of objects including linen fabrics such as beds and sheets, and disinfection of human hands.

The bactericidal cleaning agent containing the fluorine-based alcohol of the present disclosure can also be used, for example, in the same cleaning methods as those listed in the cleaning methods of the aforementioned medical appliances. As the cleaning method, any cleaning method of one type or a combination of two or more types can be used. Here, the concentration of the fluorine-based alcohol contained in the permissible bactericidal cleaning agent is the same as the concentration that kills bacteria. Specifically, it may be 0.1% by mass or more, preferably 1% by mass or more, and 8% by mass The above is preferable, and 29% by mass or more is most preferable.

The bactericidal cleaning agent containing the fluoroalcohol of the present disclosure can efficiently remove blood, body fluids, fat, and Prion protein from the target substance by being used in the preliminary cleaning process and/or the formal cleaning process of the cleaning method. Protein, PrP) and infectious amyloid-like proteins or organic matter such as cell tissues, microorganisms, viruses, bacteria, etc.

However, the bactericidal cleaning agent containing the fluorine-based alcohol of the present disclosure is not limited by the above-mentioned use, and can be compared with well-known antiviral agents, antibacterial agents, bactericides, disinfectants, and antifungals according to the cleaning target. Agents and other use. For example, the method of spraying the cleaning target, the method of coating, the method of infiltrating the target, the method of immersing the target, the method of exposing the target to high-pressure steam, and other commonly used methods can be used for this.

The temperature of the fluoroalcohol contained in the disinfectant cleaning agent of the present disclosure when killing bacteria is not particularly limited, and a temperature above normal temperature (for example, above 20°C) is preferred. If the temperature at which the fluorine-based alcohol comes into contact with the bacteria becomes higher, the bacteria will become easier to kill. In addition, the temperature may be higher than the boiling point of the fluorine-based alcohol. In short, it may be vaporized into vapor and brought into contact with bacteria. In addition, the most suitable temperature can also be selected according to the solvent or additives contained in the cleaning agent.

[Cleaning device]

In one embodiment of the cleaning device related to the present invention described above, a sterilizing cleaning agent can be used. The cleaning device can efficiently remove blood or body fluids, fat, Prion Protein (PrP), infectious amyloid, etc. from the target by supplying a sterilizing cleaning agent containing the fluoroalcohol of the present disclosure. Organic matter such as protein or cell tissue, microorganisms, viruses, bacteria, etc. The cleaning device may also use a disinfectant cleaning agent containing the fluorine-based alcohol of the present disclosure and a well-known cleaning agent in combination.

［Soil cleaning agent］

The cleaning agent related to the present invention described above can be used as a soil cleaning agent in one embodiment. By using the soil cleaning agent containing the fluorine-based alcohol of the present disclosure, the killing effect of the bacteria contained in the soil can be expected. In one embodiment, the soil cleaning agent may also contain general additives described as well-known technologies. These also include pesticides, fertilizers, fungicides, and disinfectants used in the soil.

In one embodiment, the soil cleaning agent may also include the fluorine-based alcohol and solvent disclosed in the present disclosure. The solvent may be selected from substances capable of diluting the fluorine-based alcohol, and examples thereof include the same solvents as those already listed in the aforementioned cleaning agent. These may be one or more, but are not limited to these.

In one embodiment, the content (mass%) of the solvent contained in the soil cleaning agent may be 0 mass% or more and 99.9 mass% or less relative to the mass of the soil cleaning agent from the viewpoint of cleaning performance , Preferably 0 mass% or more and 99 mass% or less, more preferably 0 mass% or more and 92 mass% or less, and more preferably 0 mass% or more and 71 mass% or less.

In one embodiment, the soil cleaning agent may contain additives in addition to the fluorine-based alcohol and solvent disclosed in the present disclosure. Examples of additives that can be added to soil cleaning agents include: surfactants, enzymes, enzyme stabilizers, anti-metal corrosion agents, low-molecular polyols, cleaning aids (detergents), defoamers, pH adjusters, fragrances , Colorants, antioxidants, preservatives, bleaching agents, bleach activators, corrosion inhibitors, dispersants, thickeners, viscosity modifiers, etc., but not limited to these. The soil cleaning agent may also contain one or more than two additives. In one embodiment, the soil cleaning agent can further improve the cleaning power by including fluorine-based alcohol, solvent and the above additives.

As the surfactant (A), for example, the same surfactants (A) listed in the aforementioned cleaning agent can be mentioned. As the surfactant (A), one type or two or more types can be used.

The content (mass%) of the surfactant (A) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is preferably 0 mass% or more and 10 mass% or less relative to the mass of the soil cleaning agent. It is more preferably 0.1% by mass or more and 5% by mass or less.

The enzyme (B) can be, for example, the same as the enzyme (B) listed in the aforementioned cleaning agent. As the enzyme (B), one kind or two or more kinds can be used.

In one embodiment, the content (mass%) of the enzyme (B) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10 mass% relative to the mass of the soil cleaning agent The following is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are especially preferable.

As the enzyme stabilizer (C), for example, the same enzyme stabilizer (D) listed in the aforementioned cleaning agent can be cited. As the enzyme stabilizer (C), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the enzyme stabilizer (C) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10% relative to the mass of the soil cleaning agent. Mass% or less is preferable, 0.05 mass% or more and 5 mass% or less are more preferable, and 0.1 mass% or more and 3 mass% or less are particularly preferable.

As the anti-metal corrosion agent (D), for example, the same ones as the anti-metal corrosion agent (F) listed in the aforementioned cleaning agent can be cited. As the anti-metal corrosion agent (D), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the anti-metal corrosion agent (D) contained in the soil cleaning agent is 0 mass% or more from the viewpoint of corrosion inhibition performance relative to the mass of the soil cleaning agent. 10% by mass or less is preferable.

Examples of the low-molecular-weight polyol (E) are the same as the low-molecular-weight polyol (G) listed in the aforementioned cleaning agent. As the low-molecular-weight polyol (E), one type or two or more types can be used.

In one embodiment, the content (mass %) of the low-molecular polyol (E) contained in the soil cleaning agent is 0 mass% or more from the viewpoint of cleaning performance relative to the mass of the soil cleaning agent. 80% by mass or less is preferable.

As the cleaning aid (washing agent) (F), for example, the same washing aids (washing agent) (H) listed in the above-mentioned washing agent can be mentioned. As the detergent (F), one type or two or more types can be used. In one embodiment, the content (mass %) of the detergent (F) contained in the soil cleaning agent is 0 mass% or more and 20% from the viewpoint of cleaning performance relative to the mass of the soil cleaning agent. The mass% or less is better.

As the defoaming agent (G), for example, the same as the defoaming agent (I) listed in the aforementioned cleaning agent can be cited. As the defoaming agent (G), one kind or two or more kinds can be used. In one embodiment, the content (mass %) of the defoaming agent (G) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 10% relative to the mass of the soil cleaning agent. The mass% or less is better.

As the pH adjuster (H), for example, the same as the pH adjuster (J) listed in the aforementioned cleaning agent can be mentioned. As the pH adjuster (H), one type or two or more types can be used. In one embodiment, the content (mass %) of the pH adjuster (H) contained in the soil cleaning agent, from the viewpoint of cleaning properties, is 0 mass% or more and 25% relative to the mass of the soil cleaning agent. Mass% or less is preferable, 0 mass% or more and 15 mass% or less are more preferable, and 0 mass% or more and 10 mass% or less are particularly preferable.

The concentration of the fluorine-based alcohol when acting on bacteria may be 0.1% by mass or more, preferably 1% by mass or more, more preferably 8% by mass or more, and particularly preferably 29% by mass or more. In addition, the time for the fluorine-based alcohol to act on the bacteria is not particularly limited. It is effective even for a short period of time for the fluorine-based alcohol to act on the bacteria. For example, the time for the fluorine-based alcohol to act on the bacteria may also be 30 seconds or more, preferably 1 minute or more, more preferably 20 minutes or more, and more preferably 30 minutes or more. In order to improve the effect, it is also better to use the method several times in segments with the interval of daily unit or weekly unit or even each season opened.

In one embodiment, the soil cleaning agent may also contain the effective ingredients of a commercially available disinfectant as an additive. As the effective ingredient of the commercially available disinfectant, for example, the same effective ingredients as those of the commercially available disinfectant listed in the aforementioned cleaning agent can be cited. In one embodiment, the content (mass%) of these active ingredients contained in the soil cleaning agent may also be 0 mass% or more and 99.9 mass% or less relative to the mass of the soil cleaning agent, with 0 mass% The above is preferably 99% by mass or less, more preferably 0% by mass or more and 92% by mass or less, and more preferably 0% by mass or more and 71% by mass or less.

In one embodiment, the fluoroalcohol contained in the soil cleaning agent, especially HFIP and TFE, are effective in cleaning soil contaminated by bacteria. HFIP and TFE are stable low-molecular compounds with high storage properties. Moreover, the cleaning temperature is not limited due to its good thermal stability, which can further enhance the cleaning power. Furthermore, HFIP and TFE are flame-retardant, and safety management in use is easy.

[cleaning method]

Using the soil cleaning agent related to this implementation type can clean soil contaminated by bacteria or soil that may be contaminated. Alternatively, the soil cleaning agent related to this implementation type can also be used preventively to clean the soil. In one embodiment, the soil cleaning agent can also be diluted with the above solvent for cleaning. In one embodiment, by adding and mixing the soil cleaning agent or solvent and soil cleaning agent related to this embodiment to the soil of the cleaning target, separating and drying the cleaning soil, the bacteria can be killed and the soil can be cleaned. .

In one embodiment, the concentration of the fluorine-based alcohol or the compound represented by the general formula (1) during cleaning may also be 0.01% by mass or more, preferably 0.1% by weight or more, and particularly preferably 1% by mass or more. The degree of soil pollution at the time of use, the period of pollution, and the surrounding environment of the soil can be adjusted arbitrarily.

In one embodiment, the fluorine-based alcohol or the compound represented by the general formula (1) can also be used as a soil fumigant. For example, by steaming the soil suspected of being contaminated with a soil fumigant containing a fluorine-based alcohol or a compound represented by the general formula (1), the soil can be cleaned.

"Example"

The following examples further illustrate the present invention in detail, but as long as it does not exceed the gist, the present invention is not limited to the following examples.

<detergent>

Prepare HFIP (1,1,1,3,3,3-hexafluoro-2-propanol, Central Glass Co., Ltd., purity 99% or more) as a compound to deactivate the virus, and EtOH (Japanese Pharmacopoeia ethanol for disinfection) , 80% of capacity) comparison. In addition, prepare TFIPL (racemate) (1,1,1-trifluoro-2-propanol, Central Glass Co., Ltd., with a purity of 99% or more), S-TFIPL (1,1,1-trifluoro- 2-Propanol, Central Glass Co., Ltd., purity over 99%), R-TFIPL (1,1,1-trifluoro-2-propanol, Central Glass Co., Ltd., purity over 99%) is used to remove the virus Activated compound.

<Cells for virus infection>

Prepare the MDBK cell line (a bovine kidney-derived cell line, Madin-Darby bovine kidney cell line, ATCC (registered trademark) CCL-22) as the cell for BVDV infection described later, as the FCV infection described later CRFK cell line (cat kidney-derived cell line, Crandell Rees feline kidney cell line, ATCC (registered trademark) CCL-94) using cells, and A549 cell line (derived from A cell line of human alveolar basal epithelial adenocarcinoma, Human Lung Epithelial cell line, ATCC (registered trademark) CCL-185), and a Vero cell line (a cell line derived from ape kidney as the cell line for enterovirus infection described later) , Cercopithecus aethiops kidney cell line, ATCC (registered trademark) CCL-81) these 4 cell lines.

The MDBK cell line is made of DMEM (Dulbecco's Modified Eagle's Medium, Dulbecco's Modified Eagle's Medium, Sigma-Aldrich Japan Co., Ltd.) containing 1% FBS (Fetal Bovine Serum, Sigma-Aldrich Japan Co., Ltd.) Company) as a culture medium, cultured under humidified, 37°C, 5% CO _{2 conditions.}

The CRFK cell line was cultured with EMEM (Eagle's minimal essential medium, Nissui Pharmaceutical Co., Ltd.) containing 2% FBS as a medium, and was cultured under _{humidified, 37°C, and 5% CO 2 conditions.}

The A549 cell line uses DMEM containing 2% FBS as a medium and is cultured under humidified, 37°C, and 5% CO ₂ conditions.

The Vero cell line uses EMEM containing 5% FBS as a medium, and is cultured under humidified, 37°C, and 5% CO ₂ conditions.

The cell culture container uses a cell culture dish (AGC TECHNO GLASS CO., LTD.) and a cell culture microplate (48-well or 96-well, AGC TECHNO GLASS CO., LTD.).

Inoculate MDBK cells or CRFK cells in a 48-well plate for cell culture, culture them at 37°C and 5% CO ₂ and use them as BVDV infection cells or FCV infection cells.

<Virus>

Bovine viral diarrhea virus (Bovine viral diarrhea virus 1, Strain: NADL, ATCC (registered trademark) VR-534, hereinafter referred to as BVDV), which is a substitute virus for hepatitis C virus, is prepared as a mantle-equipped RNA virus and strain Feline calicivirus (Feline calicivirus, Strain: F-9, ATCC (registered trademark) VR-782, hereinafter referred to as FCV), which is a substitute virus for norovirus, is an RNA virus that does not possess a mantle. In addition, the adenovirus (Human adenovirus 3, Strain: GB, ATCC (registered trademark) VR-3), which is the pathogenic virus of swimming pool fever, is prepared as a DNA virus that does not possess a mantle, and is said to be resistant to deactivation of the drug. The highly susceptible enterovirus (Human Enterovirus 71, strain: H, ATCC (registered trademark) VR-1432), which is the pathogenic virus of hand-foot-mouth disease, is an RNA virus that does not possess a mantle.

BVDV is infected with the MDBK cell line, and when more than 90% of the cultured cells show cytopathic effect (hereinafter referred to as CPE), the cultured cells together with the cell culture container are frozen and stored at -30°C. After that, the freeze-thaw operation was performed once, and the supernatant liquid centrifuged at 2380×g for 10 minutes was collected, and the virus concentrated by the ultrafiltration membrane was used as the stored virus. The stored virus solution was then used as the BVDV test virus solution.

Infect CRFK cells with FCV, and store the cultured cells together with the cell culture container at -30℃ when more than 90% of the cultured cells exhibit CPE. After that, the freeze-thaw operation was performed once, and the supernatant was collected by centrifugation at 2380×g for 10 minutes, and concentrated by ultrafiltration membrane. The concentrated virus was separated by ultracentrifugation using a sucrose cushion as the stored virus. The stored virus solution was diluted 10 times with DPBS (Dulbecco’s Phosphate buffered saline, Nissui Pharmaceutical) as the FCV test virus solution.

Infect A549 cells with adenovirus. When more than 90% of the cultured cells exhibit CPE, store the cultured cells together with the cell culture container at -30℃. After that, the freezing and thawing operation was performed once, and the supernatant was collected by centrifugation at 2380×g for 10 minutes, and concentrated by ultrafiltration membrane, and then the virus prepared by the centrifugal separation method using the sucrose concentration difference was used as the stored virus. The stored virus solution was diluted 10 times with DPBS as the adenovirus test virus solution.

Infect Vero cells with enterovirus. When more than 90% of the cultured cells exhibit CPE, the cultured cells together with the cell culture container are frozen and stored at -30°C. After that, the freeze-thaw operation was performed once, and the supernatant liquid centrifuged at 2380×g for 10 minutes was collected, and the virus concentrated by the ultrafiltration membrane was used as the stored virus. The stored virus solution is thus used as the enterovirus test virus solution.

[Example 1]

Dilute HFIP with distilled water, and use 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as the samples of Examples 1-1 to 1-5 (in addition, for each For the sample, if the volume% is converted into mass%, they are 100% by mass (stock solution), 86% by mass, 52% by mass, 29% by mass, and 8% by mass respectively. The same below.). After the samples of Example 1-1 to Example 1-5 were separated and taken out 0.9 mL to each 5 mL volume test tube, 0.1 mL of BVDV test virus liquid was added and mixed, and allowed to act at room temperature (20°C). minute. Take 0.1 mL of the action liquid and add it to 9.9 mL of SCDLP gravy medium (Eiken Chemical Co., Ltd.) as the action termination solution. The action of the sample is terminated by diluting the sample.

The BVDV test virus solution that has been subjected to the HFIP effect of Example 1-1 to Example 1-5 was diluted with DPBS from 1 to 10 ⁵ times and 10 times as a first step, and then applied to a 48-well plate inoculated with MDBK cells. Each well was inoculated with 0.1 mL (n=2). After standing for 1 hour at 37°C and 5% CO ₂ to infect the cells with the virus, the virus solution was removed, 0.5 mL of medium was added to each well, _{and cultured at 37°C and 5% CO 2} for 4 days.

[Reference example 1]

Except that ethanol was used instead of HFIP as the sample of Reference Example 1, it was carried out in accordance with Example 1. Dilute ethanol with distilled water, use 80% by volume (original solution) of ethanol as the sample of Reference Example 1-1, and use 40% by volume of ethanol as the sample of Reference Example 1-2.

[Comparative Example 1]

Except that DPBS was used instead of HFIP as the sample of Comparative Example 1, the same procedure was followed in Example 1.

[Example 2]

Except that FCV and CRFK cells were used instead of BVDV and MDBK cells as the test virus solution and virus infection cells of Example 2, the same procedure was followed in Example 1. Dilute HFIP with distilled water, and use 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as samples of Examples 2-1 to 2-5.

[Reference example 2]

Except that ethanol was used as the sample of Reference Example 2 instead of HFIP, the same procedure was followed in Example 2. Dilute ethanol with distilled water, use 80% by volume (stock solution) of ethanol as the sample of Reference Example 2-1, and use 40% by volume of ethanol as the sample of Reference Example 2-2.

[Comparative Example 2]

Except that DPBS was used instead of HFIP as the sample of Comparative Example 2, the procedure was carried out in accordance with Example 2.

[Example 3]

Except that the action time of mixing the HFIP sample of Example 2 with the FCV test virus was shortened from 30 minutes to 1 minute, the procedure was carried out in accordance with Example 2. In short, the difference between Example 2 and Example 3 is only the contact time between HFIP and FCV virus. Dilute HFIP with distilled water, and use 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as samples of Examples 3-1 to 3-5.

[Reference example 3]

Except that ethanol was used instead of HFIP as the sample of Reference Example 3, the procedure was carried out in accordance with Example 3. Dilute ethanol with distilled water, use 80% by volume (stock solution) of ethanol as the sample of Reference Example 3-1, and use 40% by volume of ethanol as the sample of Reference Example 3-2.

[Comparative Example 3]

Except that DPBS was used instead of HFIP as the sample of Comparative Example 3, the procedure was carried out in accordance with Example 3.

[Example 4]

Except that adenovirus and A549 cells were used instead of BVDV and MDBK cells as the test virus solution and virus infection cells of Example 4, and the action time was set at 1 minute, the procedure was carried out in accordance with Example 1. Dilute HFIP with distilled water, and use 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as samples of Examples 4-1 to 4-5.

[Reference example 4]

Except that ethanol was used instead of HFIP as the sample of Reference Example 4, the same procedure was followed in Example 4. Dilute ethanol with distilled water, use 80% by volume (stock solution) of ethanol as the sample of Reference Example 4-1, and use 40% by volume of ethanol as the sample of Reference Example 4-2.

[Comparative Example 4]

Except that DPBS was used instead of HFIP as the sample of Comparative Example 4, the procedure was carried out in accordance with Example 4.

[Example 5]

Except that enterovirus and Vero cells were used instead of BVDV and MDBK cells as the test virus solution and virus infection cells of Example 5, and the action time was set to 1 minute, the procedure was carried out in accordance with Example 1. Dilute HFIP with distilled water, and use 100% by volume (stock solution), 80% by volume, 40% by volume, 20% by volume, and 5% by volume of HFIP as samples of Examples 5-1 to 5-5.

[Reference example 5]

Except that ethanol was used instead of HFIP as the sample of Reference Example 5, the procedure was carried out in accordance with Example 5. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 5-1. In addition, because enterovirus is a virus that is not easily deactivated, 40% ethanol as a reference example has not been prepared.

[Comparative Example 5]

Except that DPBS was used as the sample of Comparative Example 5 instead of HFIP, the same procedure was followed in Example 5.

<Evaluation of virus deactivation caused by HFIP>

The evaluation of virus inactivation caused by HFIP is carried out by observing the type of cells infected with HFIP virus using a microscope. In Figures 2 to 6, black dots (●) indicate those who can confirm CPE caused by virus proliferation (virus-infected cells), and white dots (○) indicate those who have not confirmed CPE (no virus) Infected cells). In addition, a white triangle (△) indicates that the cytotoxicity can be seen but CPE caused by the proliferation of the virus has not been confirmed (cells not infected by the virus), and a black triangle (▲) indicates that the cytotoxicity can be seen and confirmed To CPE caused by the proliferation of the virus (cells that have been infected with the virus). In addition, "%" in Figs. 2 to 6 means "capacity%".

As shown in Fig. 2 to Fig. 6, compared with the comparative example, the virus deactivation effect was observed in all the examples and the reference example.

As shown in Fig. 2, for BVDV, compared with Reference Example 1-2, a stronger virus inactivation effect was observed in Examples 1-1 to 1-5 and Reference Example 1-1. In other words, it means that in HFIP above 5 volume %, there is a stronger BVDV deactivation effect than 40 volume% EtOH.

As shown in Fig. 3, for FCV, compared with Reference Example 2-2, a strong virus deactivation effect was observed in Examples 2-1 to 2-5 and Reference Example 2-1. Furthermore, in Example 2-1 to Example 2-4, a stronger virus deactivation effect was observed than in Example 2-5 and Reference Example 2-1. In other words, it means that in HFIP with a volume of 20% or more, the FCV deactivation effect is stronger than that of 5% by volume of HFIP and 80% by volume of EtOH.

As shown in Figure 4, Examples 3-1 to 3-5, which shortened the action time of HFIP on FCV, showed stronger virus deactivation than Comparative Example 3, Reference Example 3-1, and Reference Example 3-2. Effect. In other words, it means that in HFIP with a volume of more than 5% by volume, the FCV deactivation effect is stronger than that of 40% by volume EtOH and 80% by volume EtOH.

As shown in FIG. 5, for adenovirus, compared with Reference Example 4-2, a strong virus inactivation effect was observed in Examples 4-1 to 4-5 and Reference Example 4-1. In other words, it means that in HFIP above 5% by volume, there is a stronger effect of deactivating adenovirus than 40% by volume EtOH.

As shown in Fig. 6, for enteroviruses, compared with Reference Example 5-1, a strong virus inactivation effect was observed in Examples 5-1 to 5-5. Especially in Example 5-1 to Example 5-3, a strong virus deactivation effect was observed. That is, in HFIP above 5% by volume, the adenovirus deactivation effect is stronger than that with 80% by volume EtOH. Especially in HFIP above 40% by volume, its deactivation effect is significant.

Regarding those who have observed cytotoxicity in the examples, if the purpose of cleaning for medical appliances is considered, the presence or absence of cytotoxicity is not the one that has an influence on the cleaning effect.

[Example 6]

Except that S-TFIPL was used instead of the HFIP of Example 3 as the sample of Example 6, the same procedure was followed in Example 3. Dilute S-TFIPL with distilled water, and use 100% by volume (stock solution), 40% by volume, and 20% by volume of S-TFIPL as the samples of Examples 6-1 to 6-3 (In addition, for each sample, if The volume% is converted into mass%, and they are 100% by mass (stock solution), 46% by mass, and 24% by mass. The same below.).

[Reference example 6]

Except that ethanol was used instead of S-TFIPL as the sample of Reference Example 6, the same procedure was followed in Example 6. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 6.

[Comparative Example 6]

Except that DPBS was used instead of S-TFIPL as the sample of Comparative Example 6, the same procedure was followed in Example 6.

[Example 7]

Except that TFIPL (racemate) was used instead of S-TFIPL as the sample of Example 7, the same procedure was followed in Example 6. Dilute TFIPL (racemate) with distilled water, and use 100% by volume (stock solution), 40% by volume, and 20% by volume of TFIPL (racemate) as the samples of Examples 7-1 to 7-3 ( In addition, for each sample, if the volume% is converted into mass %, they are 100 mass% (stock solution), 46 mass %, and 24 mass %. The same applies hereinafter.).

[Reference example 7]

Except that ethanol was used instead of TFIPL (racemate) as the sample of Reference Example 7, the same procedure was followed in Example 7. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 7.

[Comparative Example 7]

Except that DPBS was used instead of TFIPL (racemate) as the sample of Comparative Example 7, the same procedure was followed in Example 7.

[Example 8]

Except that S-TFIPL was used instead of HFIP as the sample of Example 8 and the action time was shortened from 30 minutes to 1 minute, the procedure was carried out in accordance with Example 1. Dilute S-TFIPL with distilled water, and use 100% by volume (stock solution), 40% by volume, and 20% by volume of S-TFIPL as the samples of Examples 8-1 to 8-3 (in addition, for each sample, if the The volume% is converted into mass%, and they are 100% by mass (stock solution), 46% by mass, and 24% by mass. The same below.).

[Reference Example 8]

Except that ethanol was used instead of S-TFIPL as the sample of Reference Example 8, the same procedure was followed in Example 8. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 8.

[Comparative Example 8]

Except that DPBS was used instead of S-TFIPL as the sample of Comparative Example 8, the same procedure was followed in Example 8.

[Example 9]

Except that R-TFIPL was used instead of S-TFIPL of Example 8 as the sample of Example 9, the same as Example 8. The R-TFIPL was diluted with distilled water, and 100% by volume (stock solution), 40% by volume, and 20% by volume of R-TFIPL were used as samples of Examples 9-1 to 9-3.

[Reference Example 9]

Except that ethanol was used instead of R-TFIPL as the sample of Reference Example 9, the same procedure was followed in Example 9. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 9.

[Comparative Example 9]

Except that DPBS was used instead of R-TFIPL as the sample of Comparative Example 9, the same procedure was followed in Example 9.

[Example 10]

Except that TFIPL (racemate) was used instead of R-TFIPL of Example 9 as the sample of Example 10, the same procedure was followed in Example 9. The TFIPL (racemate) was diluted with distilled water, and 100% by volume (stock solution), 40% by volume, and 20% by volume of TFIPL (racemate) were used as samples of Examples 10-1 to 10-3.

[Reference Example 10]

Except that ethanol was used instead of TFIPL (racemate) as the sample of Reference Example 10, the same procedure was followed in Example 10. The ethanol was diluted with distilled water, and 80% by volume (stock solution) of ethanol was used as the sample of Reference Example 10.

[Comparative Example 10]

Except that DPBS was used instead of TFIPL (racemate) as the sample of Comparative Example 10, the same procedure was followed in Example 10.

<Evaluation of virus deactivation caused by TFIPL>

The evaluation of virus inactivation caused by TFIPL is carried out by observing the type of cells infected with the virus that has been acted on by TFIPL using a microscope. In Figures 7 to 11, black dots (●) indicate those who can confirm CPE caused by virus proliferation (virus-infected cells), and white dots (○) indicate those who have not confirmed CPE (no virus) Infected cells). In addition, a white triangle (△) indicates that the cytotoxicity can be seen but CPE caused by the proliferation of the virus has not been confirmed (cells not infected by the virus), and a black triangle (▲) indicates that the cytotoxicity can be seen and confirmed To CPE caused by the proliferation of the virus (cells that have been infected with the virus). In addition, "%" in FIGS. 7 to 11 represents "capacity%".

As shown in FIGS. 7 to 11, compared with the comparative example, the effect of virus inactivation was observed in all the examples and the reference example.

As shown in FIG. 7, for Examples 6-1 to 6-3 in which FCV was acted on by S-TFIPL, compared with Reference Example 6, a strong virus inactivation effect was confirmed. That is, it means that in S-TFIPL of 20% by volume or more, there is a stronger FCV deactivation effect than 80% by volume of EtOH.

As shown in FIG. 8, for Examples 7-1 to 7-3 in which FCV was acted on by TFIPL (racemate), compared with Reference Example 7, a strong virus inactivation effect was confirmed. Especially in Example 7-1 and Example 7-2, a strong virus inactivation effect was observed. In other words, it means that in TFIPL (racemate) of 20% by volume or more, there is a stronger FCV deactivation effect than 80% by volume of EtOH.

As shown in FIG. 9, compared with Comparative Example 8 in Examples 8-1 to 8-3 in which BVDV was acted on by S-TFIPL, a strong virus inactivation effect was confirmed. That is, it means that there is a BVDV deactivation effect in S-TFIPL above 20% by volume.

As shown in FIG. 10, for Examples 9-1 to 9-3 in which BVDV was acted on by R-TFIPL, compared with Comparative Example 9, a strong virus inactivation effect was confirmed. That is, it means that there is a BVDV deactivation effect in R-TFIPL of more than 20% by volume.

As shown in FIG. 11, compared with Comparative Example 10, in Examples 10-1 to 10-3 in which TFIPL (racemate) of BVDV acts, a strong virus inactivation effect was confirmed. That is, it means that there is a BVDV deactivation effect in TFIPL (racemate) of more than 20% by volume.

[Protein solubility test]

Dilute HFIP with distilled water, use 100% (original solution), 80%, 40%, 20%, and 5% of HFIP water solution to implement the cleaning evaluation indicator for medical equipment (TOSI, Songji Medical Instruments Co., Ltd.) Co., Ltd.) cleaning evaluation of suspected human plasma protein contaminants. After immersing TOSI in the HFIP aqueous solution of each concentration and performing ultrasonic cleaning at 20°C for 10 minutes, the residual protein detection solution (manufactured by Saraya Co., Ltd., Power Quick) was used to confirm the presence or absence of residual protein on TOSI. In the case of using any concentration of HFIP aqueous solution, no residual protein was detected. In other words, it can be seen that in HFIP of more than 5% by volume, suspected contaminants on TOSI can be removed.

[Bactericidal evaluation test]

<Inoculation strain>

Escherichia coli NBRC3972 strain (E. coli) was used as the strain.

<Measuring Medium>

The measurement medium used tryptic soy agar medium (Becton, Dickinson).

<Preparation of Evaluation Solution>

Use sterilized water to dilute the evaluation sample and adjust to any concentration.

<Preparation of inoculum solution>

After culturing the bacteria in the previous medium overnight, scrape the bacteria to become 10 ⁸ cfu/mL, and prepare the inoculation bacterial solution with 0.85% by weight of saline containing 0.1% by weight of tryptic protein.

[Example 11-1]

Take 10 μL of the inoculum solution into the micro test tube, add 190 μL of the evaluation sample solution: 80% HFIP into the micro test tube and let it act for 30 seconds, then take 10 μL of the reaction solution and use 0.1% by weight of tryptic protein. 0.85 wt% of normal saline is diluted to 100 times to stop the action of the sample (this solution is called the reaction stop solution). Use 0.85 wt% physiological saline containing 0.1 wt% tryptic protein to dilute the reaction stop solution to 10,000 times in 10-fold units. Separate 100 μL from each diluted solution, and apply to the pre-medium separately. Incubate them in an incubator at 35°C overnight, and measure the number of colonies grown by naked eyes.

<Evaluation Method of Sterilization>

Calculate the common logarithmic value of the number of bacteria (CFU/mL) contained in the inoculum solution and its average value. From this average value and the average value of the logarithmic value of the number of bacteria (CFU/mL) after the evaluation of the sample solution, the log reduction value (Log Reduction Value: LR value) is calculated by the following formula. Log Reduction=A-B A: The average value of the number of bacteria (commonly used logarithmic value) contained in the inoculum solution B: The average value of the number of bacteria (commonly used logarithmic value) after the evaluation of the sample solution

[Example 11-2]

Except that the evaluation sample liquid was set to 40% HFIP, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Example 11-3]

Except that the evaluation sample liquid was set to 20% HFIP, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Example 11-4]

Except that the evaluation sample liquid was set to 10% HFIP and the reaction termination liquid was diluted to 1,000 times in units of 10 times, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Example 11-5]

Except that the evaluation sample liquid was set to 5% HFIP and the reaction termination liquid was diluted to 1,000 times in 10-fold units, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Example 11-6]

Except that the evaluation sample liquid was set to 2.5% HFIP and the reaction termination liquid was diluted to 1,000 times in 10-fold units, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Example 12-1]

The bactericidal evaluation test was carried out in the same procedure as in Example 11-1, except that the evaluation sample liquid was set to 80% TFE (in addition, if the volume% is converted to mass %, it is 85% by mass TFE. The same hereinafter.).

[Example 12-2]

The bactericidal evaluation test was carried out in the same procedure as in Example 12-1 except that the evaluation sample liquid was set to 40% TFE (in addition, if the volume% is converted to mass %, it is 48% by mass TFE. The same hereinafter.).

[Example 12-3]

The bactericidal evaluation test was performed in the same procedure as in Example 12-1, except that the evaluation sample liquid was set to 20% TFE (in addition, if the volume% is converted to mass %, it is 26% by mass TFE. The same hereinafter.).

[Example 12-4]

Except that the evaluation sample solution is set to 10% TFE (in addition, if the volume% is converted to mass%, it is 13 mass% TFE. The same below.) and the reaction termination solution is diluted to 1,000 times by 10 times. The bactericidal evaluation test was carried out in the same procedure as in Example 12-1.

[Comparative Example 11]

The bactericidal evaluation test was carried out in the same procedure as in Example 11-1 except that the evaluation sample liquid was set to physiological saline.

[Reference Example 11-1]

Except that the evaluation sample liquid was set to 80% EtOH, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Reference example 11-2]

Except that the evaluation sample liquid was set to 40% EtOH, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

[Reference example 11-3]

Except that the evaluation sample liquid was set to 20% EtOH, the sterilization evaluation test was carried out in the same procedure as in Example 11-1.

For Example 11-1 to Example 11-6, Example 12-1 to Example 12-4, Comparative Example 11 and Reference Example 11-1 to Reference Example 11-3, the concentration of inoculation bacteria was collected and the sample solution was evaluated , The contact time, and the number of colonies after culturing at each dilution rate are shown in Figure 12. In Figure 12, "-" indicates that it has not been implemented. In addition, in FIG. 13, for each evaluation sample liquid, the bactericidal efficacy is represented by an LR value (Log Reduction Value). For each evaluation sample solution, those with an LR value of 3.0 or more (the number of bacteria reduced by 99.9% or more) is judged as "◎", and those with an LR value of less than 1.0 are judged as "╳".

As shown in Figure 13, HFIP and TFE have obvious bactericidal effects on Escherichia coli. In particular, the comparison between Reference Example 11-3 and Example 11-3 and the comparison between Reference Example 11-3 and Example 12-3 show that HFIP and TFE exhibit stronger bactericidal properties than EtOH. In this embodiment, the bactericidal effect on Escherichia coli is disclosed, but it is conceivable that fluoroalcohols such as HFIP and TFE have effects on other bacteria, such as: Staphylococcus aureus, Escherichia coli, enterohemorrhagic Escherichia coli, tuberculosis, MRSA, Vancomycin-resistant enterococci, multi-drug resistant Pseudomonas aeruginosa, multi-drug resistant Acinetobacter, etc. also show the same bactericidal effect.

The cleaning agent for deactivating viruses in the present disclosure can be advantageously used for cleaning medical appliances and the like.

1: Cleaning device 10: Cleaning tank 12: Water storage department 14: Water supply pipe 16: Hot water supply pipe 18: Cleaning the pump 20: Storage department 22: Cleaning the nozzle 34: Drain pipe 40: Cleaning agent supply device

<Figure 1> is a cross-sectional view illustrating the structure of a cleaning device related to an embodiment of the present disclosure.

<Figure 2> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 3> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 4> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 5> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 6> is a diagram showing the evaluation of virus deactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 7> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 8> is a diagram showing the evaluation of virus deactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 9> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 10> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 11> is a diagram showing the evaluation of virus inactivation of the cleaning agent related to an embodiment of the present disclosure.

<Figure 12> is a diagram showing the evaluation of the sterilization of the cleaning agent related to an embodiment of the present disclosure.

<Figure 13> is a diagram showing the evaluation of the sterilization of the cleaning agent related to an embodiment of the present disclosure.

1: Cleaning device

10: Cleaning tank

12: Water storage department

14: Water supply pipe

16: Hot water supply pipe

18: Cleaning the pump

20: Storage department

22: Cleaning the nozzle

34: Drain pipe

40: Cleaning agent supply device

Claims (46)

A cleaning agent containing fluorine-based alcohol as a compound for deactivating viruses. The cleaning agent according to claim 1, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually exclusive Different or the same. The cleaning agent according to claim 1, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning agent according to claim 1, wherein the concentration of the fluorine-based alcohol when acting on the virus is 0.1% by mass or more. The cleaning agent according to claim 1, which further contains a solvent. The cleaning agent according to claim 1, wherein the aforementioned virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus and enterovirus. The cleaning agent described in claim 1, which is used for cleaning medical appliances. The cleaning agent described in claim 1, which is used for soil cleaning. A cleaning method which includes causing a fluorine-based alcohol to act on a virus. The cleaning method according to claim 9, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually Different or the same. The cleaning method according to claim 9, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning method according to claim 9, wherein the concentration of the fluoroalcohol when acting on the virus is 0.1% by mass or more. The cleaning method according to claim 9, wherein the fluorine-based alcohol is diluted with a solvent. The cleaning method according to claim 9, wherein the aforementioned virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus, and enterovirus. The cleaning method according to claim 9, which is used for cleaning medical appliances. The cleaning method described in claim 9, which is used for soil cleaning. A cleaning device comprising: a cleaning agent supply device that supplies a cleaning agent containing a fluorine-based alcohol as a compound that deactivates viruses. The cleaning device according to claim 17, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually Different or the same. The cleaning device according to claim 17, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning device according to claim 17, wherein the concentration of the fluorine-based alcohol when acting on the virus is 0.1% by mass or more. The cleaning device according to claim 17, wherein the fluorine-based alcohol is diluted with a solvent. The cleaning device according to claim 17, wherein the aforementioned virus is at least one selected from the group consisting of influenza virus, hepatitis C virus, rotavirus, norovirus, adenovirus, and enterovirus. The cleaning device according to claim 17, which is used for cleaning medical appliances. The cleaning device according to claim 17, which is used for soil cleaning. A cleaning agent containing fluorine-based alcohol as a compound that kills bacteria. The cleaning agent according to claim 25, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually Different or the same. The cleaning agent according to claim 25, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning agent according to claim 25, wherein the concentration of the fluorine-based alcohol when acting on the bacteria is 0.1% by mass or more. The cleaning agent according to claim 25, which further contains a solvent. The cleaning agent according to claim 25, which is used for cleaning medical appliances. The cleaning agent according to claim 25, which is used as a disinfectant. The cleaning agent described in claim 25, which is used for soil cleaning. A cleaning method which includes causing a fluorine-based alcohol to act on bacteria. The cleaning method according to claim 33, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually exclusive Different or the same. The cleaning method according to claim 33, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning method according to claim 33, wherein the concentration of the fluorine-based alcohol when acting on the bacteria is 0.1% by mass or more. The cleaning method according to claim 33, wherein the fluorine-based alcohol is diluted with a solvent. The cleaning method described in claim 33, which is used for cleaning medical appliances. The cleaning method described in claim 33 is used for soil cleaning. A cleaning device comprising: a cleaning agent supply device that supplies a cleaning agent containing a fluorine-based alcohol as a compound that kills bacteria. The cleaning device according to claim 40, wherein the aforementioned fluorine-based alcohol is represented by the general formula RfCH ₂ OH or RfRf'CHOH, Rf and Rf' represent a perfluoroalkyl group having 1 to 10 carbon atoms, and Rf and Rf' are mutually Different or the same. The cleaning device according to claim 40, wherein the aforementioned fluorine-based alcohol is selected from 1,1,1,3,3,3-hexafluoro-2-propanol, 2,2,2-trifluoroethanol and 2, At least one of the group consisting of 2,3,3,3-pentafluoro-1-propanol. The cleaning device according to claim 40, wherein the concentration of the fluorine-based alcohol when acting on the bacteria is 0.1% by mass or more. The cleaning device according to claim 40, wherein the fluorine-based alcohol is diluted with a solvent. The cleaning device according to claim 40, which is used for cleaning medical appliances. The cleaning device according to claim 40, which is used for soil cleaning.

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