TW201438671A - Method for forming brain stimulation cognitive response images - Google Patents

Abstract

The present invention relates to a method for forming brain stimulation cognitive response images, in which a subject is set under control of eye-closed condition, visual condition, auditory condition, and cognitive condition, and glucose molecules with carbon 11 isotopes are injected into the body of the subject, which is then subjected to brain imaging to observe the brain activities in response to the change of the injected glucose molecules with carbon 11 isotopes when the subject does not receive external stimuli or the subject receives various external stimuli. As such, the metabolic process of the glucose in the bran can be inspected to reflect the brain function images of a human brain in response to visual, auditory, and cognitive stimulations.

Description

A method for stimulating brain cognitive response image formation

The invention relates to a method for stimulating image formation of brain cognition response, in particular to using a carbon 11-2-deoxy-D-glucose to stimulate brain cognition response image formation through external conditions such as visual, auditory or cognitive stimulation. Novel approach.

The brain is an important central nervous system for human beings. It is responsible for the five functions of human spirit, thinking, body sense, hearing and vision. It is also the place where human beings produce psychological phenomena. The human brain structure is divided into the brain and the cerebellum. The brain is made up of the cerebral cortex (the neocortex of the brain), the marginal leaves of the brain (the old cortex), the brain stem, and the brain. The cerebral cortex can be divided into three parts: the frontal lobe, the Nieye and the occipital lobe. In the past, researchers' brain activity scanning methods for mental model changes can only rely on, for example, electroencephalograph (EEG), brain magnetic wave (MEG), Positron Emission Tomography (PET), single photon computed tomography. (Single Photon Emission Tomography, SPECT) and functional magnetic resonance imaging (fMRI) techniques to explore the cognitive function of the brain.

In the past, many analytical methods for acquiring brain images using brain angiography have presupposed that we have measured changes in hemodynamic response (HR) with experimental stimuli or related events, which occur in several parts of the brain. On the network. These analysis methods reflect that our understanding of the brain is still in the paradigm of linear systems: the simplification of the meaning of the brain image "voxel" based on the framework of the modular theory, the theory holds "Different brain regions are responsible for different cognitive functions," and believe that the hemodynamic changes and non-HR noise we see in brain images are still homogenous in different events and different brain regions. But as our understanding of the brain becomes more complex, we can recognize the unpredictability of HR changes and the complexity of the brain.

In terms of functional magnetic resonance imaging and positron tomography, although the two brain imaging techniques have high spatial resolution, they have the disadvantage of low time resolution, and the brain wave instrument and the brain magnetic wave instrument can measure the activation of nerve tissue. The magnetic field distribution and electric field distribution generated by the ion current flow have the advantages of superior millisecond and high time resolution, which can be used to explore the sequential rotation of brain network neuron activity, and the method of transcranial magnetic stimulation (Transcranial) Magnetic Stimulation (TMS) has a lot of room to modulate the plasticity of the neural network. However, these techniques do not have the high spatial resolution of functional magnetic resonance imaging or positron tomography. Since these methods are based on different medical physics mechanisms, the resulting image or signal information has different characteristics.

In terms of the technique of using radiopharmaceuticals to assist brain angiography, the most commonly used radioactive drug for fluorine 18, such as [Fluoro 18]-2-deoxy-D-glucose (F-18 Fluoro-2-Deoxyglucose, F -18-FDG), also known as fluorinated deoxyglucose, is a molecule that is very similar to the chemical structure of glucose. The distribution of fluorinated deoxyglucose in the organism can vary depending on the absorption and metabolism of glucose by tissues and organs. After the positron angiography, the physiological activity image of the glucose molecule in the human body is presented, and the diagnostic function is achieved through a quantitative standard uptake value (SUV). Due to the rapid division of the cancer cells that are proliferating, the metabolic rate of the cells is abnormally increased by the regulation of the gene protein, so the fluorescein deoxyglucose can show a higher difference than the background absorption of the normal cells. Photograph taken by positron angiography Like a molecular phenomenon that can reveal organs or tissues.

However, [Fluoro 18]-2-deoxyglucose (F-18-FDG) has a half-life of up to 109 minutes due to the fluorine 18 isotope, for brain psychology, behavior, and cognition. The measurement time may cause too long. Therefore, in addition to fluorinated deoxyglucose, the development of new drug molecules for the application of brain imaging technology to break through the current technical bottleneck in the field of brain imaging is the direction of the development of the technology field.

In order to achieve the above object, the technical means used in the method of the present invention include stimulating the brain in three ways: visual, auditory, and cognitive (Cognitive), and observing the glucose molecule having the carbon 11 isotope. A method for changing a brain in a subject, wherein the visual method comprises at least the following steps: under the control of the closed eye condition, the subject is closed in an environmental condition without any sound and dim light, so that the pupil of the eye is unacceptable Any external image, no problem is considered in the whole process, and nothing is imagined; the subject is injected with glucose molecules with carbon 11 isotope; the subject is brain-enhanced and displayed in the display unit of the contrast instrument a first moving image (a) of a glucose molecule having a carbon 11 isotope in the brain of the subject, the first moving image (a) being used as a standard absorption value; subjecting the visual stimulation condition to a subject In an environment without any sound and soft lighting, open your eyes all the time, let the eyeball pupil accept a soft color test room or test room with music related No musical instruments and video, image or make the environment outside the testing room of pastel colors arranged music related symbols and musical instruments image presented in front of the pupil, full open eyes And imagining a familiar music melody; injecting a glucose molecule having a carbon 11 isotope into the subject; performing brain imaging on the subject, and displaying the glucose molecule having the carbon 11 isotope in the display unit of the angiography instrument at the test The second moving image (b) in the brain; comparing the first moving image (a) with the second moving image (b), observing the subject before the visual stimulation condition is controlled, the brain a change in a glucose molecular active block having a carbon 11 isotope; wherein the second active image (b) exhibits a glucose molecular activity having a carbon 11 isotope in the brain when the subject opens both eyes and imagines a musical melody Preferably, the glucose molecule having a carbon 11 isotope is carbon 11-2-deoxy-D-glucose; preferably, the visual stimulation condition may be, but not limited to, a single soft color test room, a different composite The test room and the test room of the color system are arranged in the form of music-related symbols and musical instrument images; preferably, the technique of brain imaging is Positron Emission Tomography (PET) or single photon imaging (Single Phot). On Emission Tomography, SPECT).

In another aspect, the invention also includes a method of using auditory stimuli, comprising at least the steps of: subjecting the subject to control under closed eye conditions, not thinking about any problem, nor imagining anything; injecting the subject with carbon 11 a glucose molecule of an isotope; brain angiography of the subject, and displaying in the display unit of the angiography apparatus a third moving image (c) of the glucose molecule having the carbon 11 isotope in the brain of the subject, The third activity image (c) is used as a standard absorption value; Under the control of auditory stimulation conditions, the subjects can open their ears (the ear canal without earplugs and other obstacles) and open their eyes in the environment without any sound and soft lighting. The test room plays music or CD in the form of CD or the like. Artificial play and other forms, let the ears accept the soft music melody familiar and loved by the subject; inject the glucose molecule with carbon 11 isotope into the subject; perform brain imaging on the subject and display it on the contrast instrument A fourth moving image (d) of the glucose molecule having a carbon 11 isotope in the brain of the subject is displayed in the unit; comparing the fourth moving image (d) with the third moving image (c), The change in the active region of the glucose molecule with carbon 11 isotope in the brain was observed after the subject was controlled by the auditory stimulation condition.

Wherein, the fourth activity image (d) presents a moving image of a glucose molecule having a carbon 11 isotope in the brain when the subject opens the eyes and listens to the music melody; preferably, the glucose having the carbon 11 isotope The molecule is carbon 11-2-deoxy-D-glucose; preferably, the auditory stimulation condition may be in the form of, but not limited to, playing music in the form of CD or the like, live playing in the field, etc.; preferably, the technique of brain imaging is Positron Emission Tomography (PET) or Single Photon Emission Tomography (SPECT).

In another aspect, the invention also includes a method of using cognitive stimuli, comprising at least the steps of: subjecting the subject to control under closed eye conditions, not thinking about any problem, nor imagining anything; injecting the subject with carbon 11 Isotopic glucose molecule; brain imaging of the subject and display in the display unit of the contrast instrument The fifth active image (e) of the glucose molecule having the carbon 11 isotope in the brain of the subject, the fifth active image (e) being the standard absorption value; and subjecting the cognitive stimulus condition to the test In the environment without any sound and soft lighting, open the ears (the ear canal without earplugs and other obstacles) and open the eyes, the subject in the test room with their own hands to play in the imaginary form, or with both feet In the form of melody, playing the soft music melody familiar and loved by the subject; injecting the glucose molecule with carbon 11 isotope into the subject; performing brain imaging on the subject and in the display unit of the contrast instrument Showing a sixth moving image (f) of the glucose molecule having a carbon 11 isotope in the brain of the subject; comparing the sixth moving image (f) with the fifth moving image (e), observing Subjects had changes in the active regions of glucose molecules with carbon 11 isotopes in the brain before and after control of cognitive stimulation conditions.

Wherein, the sixth activity image (f) presents a moving image of a glucose molecule having a carbon 11 isotope in the brain when the subject opens the eyes and imagines the hand playing the piano and the finger playing the music melody with the beat action; Preferably, the glucose molecule having a carbon 11 isotope is carbon 11-2-deoxy-D-glucose; preferably, the cognitive stimulation condition may be, but is not limited to, the subject using his own hands in an imaginary form in the test room. Playing, or in the form of two-legged melody, playing the soft music melody familiar and loved by the subject; preferably, the technique of brain imaging is Positron Emission Tomography (PET) Or single photon imaging (Single Photon Emission Tomography, SPECT).

The invention is a continuation of the traditional nuclear medicine tracer imaging technology concept, and is a novel method for stimulating brain cognitive response image formation through visual hearing, by the above technical means The invention provides a novel brain cognitive reaction method, which utilizes carbon 11-2-deoxy-D-glucose ([1-C-11]2-Deoxy-D-Glucose, abbreviated as C-11-2DG). The contrast agent injects the carbon-11 positron decay isotope marker drug C-11-2DG into the body, and when the released positron encounters the electrons of the cell, an "annihilation reaction" occurs, forming a pair of 511 KeV plus After the ray is measured by the positron imager, the image is reconstructed by computer operation. Furthermore, the present invention relates to an innovative research on the drug development and psychological cognition of the functional parts of the brain in the nuclear medicine industry, in addition to the new use of drugs in the nuclear medicine industry, in the fields of psychological cognition and management strategies. The technology of the present invention may also be indispensable.

(a) ‧ ‧ first activity image

(b) ‧ ‧ second activity image

(c) ‧ ‧ third activity image

(d) ‧ ‧ fourth event image

(e) ‧ ‧ fifth event image

(f) ‧ ‧ sixth activity image

Fig. 1 is a comparison diagram of changes in glucose molecular activity blocks before and after stimulation of the brain in the method of the present invention.

The present invention is a method for stimulating brain cognitive response image formation, which comprises the following steps:

Step 11: Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

Step 12: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 13: performing brain imaging on the subject, and displaying a first active image (a) of the glucose molecule having the carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, the first activity Image (a) is used as a standard absorption value, according to the present invention, brain angiography Techniques can be, but are not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and other alternative techniques.

Step 14: The subject opens the eyes and imagines the music melody in the whole process under the control of the visual stimulation condition. According to the present invention, the visual stimulation condition may be, but not limited to, a test room of a single soft color system, and a composite color system. The test room and the test room are arranged in the form of music-related symbols and musical instrument images; in addition, the imaginary music melody is preferably a musical melody that is well known to the subject.

Step 15: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 16: performing brain imaging on the subject, and displaying a second moving image (b) of the glucose molecule having the carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, according to the present invention, Techniques for brain imaging may be, but are not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and other alternative techniques. .

Step 17: The second moving image (b) is compared with the first moving image (a) to observe changes in the glucose molecule activity of the carbon 11 isotope in the brain before and after the control of the visual stimulation condition.

In another aspect, the technical means utilized by the method of the present invention may also include the following steps:

Step 21: Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

Step 22: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 23: performing brain imaging on the subject, and displaying a third active image (c) of the glucose molecule having carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, this third The moving image (c) is used as a standard absorption value. According to the present invention, the technique of brain imaging can be, but not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and the like. Alternative technology.

Step 24: The subject opens the eyes and listens to the music melody in the whole process under the control of the auditory stimulation condition. According to the present invention, the auditory stimulation condition may be, but is not limited to, playing music in the form of CD or the like, and playing in the field; In addition, the music the subject is listening to is better known to the music that the subject himself is familiar with.

Step 25: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 26: performing brain imaging on the subject, and displaying a fourth moving image (d) of the glucose molecule having the carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, according to the present invention The technique of brain imaging may be, but not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and other alternative technologies.

Step 27: The fourth moving image (d) is compared with the third moving image (c), and the subject is observed to have a carbon 11 isotope in the brain before and after the auditory stimulation condition is controlled. Changes in the activity of glucose molecules.

In yet another aspect, the technical means utilized by the method of the present invention may also include the following steps:

Step 31: Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

Step 32: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 33: performing brain imaging on the subject, and displaying a fifth moving image (e) of the glucose molecule having a carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, the fifth The moving image (e) is used as a standard absorption value. According to the present invention, the technique of brain imaging can be, but not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and the like. Alternative technology.

Step 34: Under the control of the cognitive stimulation condition, the subject opens the eyes and imagines the hand playing the piano, and the finger plays the music melody with the beat action. According to the present invention, the cognitive stimulation condition may be but not limited to the measured In the test room, play with his own hands in the form of imaginary, or in the form of two-legged melody, playing the soft music melody familiar and loved by the subject; and the music the subject listens to. Music is well known to the subject itself.

Step 35: The subject is injected with a glucose molecule having a carbon 11 isotope. According to the present invention, the glucose molecule having the carbon 11 isotope may be carbon 11-2-deoxy-D-glucose or other alternative substance.

Step 36: performing brain imaging on the subject, and displaying a sixth moving image (f) of the glucose molecule having carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument, according to the present invention The technique of brain imaging may be, but not limited to, Positron Emission Tomography (PET), Single Photon Emission Tomography (SPECT), and other alternative technologies.

Step 37: comparing the sixth activity image (f) with the fifth activity image (e), observing the activity of the glucose molecule having a carbon 11 isotope in the brain before and after the cognitive stimulation condition is controlled. Variety.

Glucose is the main source of energy for brain activity. The metabolism of glucose inside the brain can reflect the state of brain function to a certain extent. A decrease in the level of glucose metabolism may indicate inhibition of local neuronal activity in the brain, which is one of the manifestations of brain dysfunction. As a glucose analog, such as carbon 11-2-deoxy-D-glucose, it will be taken up by high-yield glucose cells in brain cells. In such cells, phosphorylation will prevent glucose molecules from becoming intact. The form is released from the cell.

Since the oxygen at the 2' position in the molecular structure of the glucose is necessary for subsequent glycolysis, the carbon 11-2-deoxy-D-glucose in the cell cannot continue to be metabolized, and before the radioactive decay, the carbon 11-2 - Carbon 11-2-deoxy-D-glucose-6-phosphate formed by dephosphorylation of D-glucose will not undergo glycolysis. Thereby, the distribution of carbon 11-2-deoxy-D-glucose appropriately reflects the uptake of glucose molecules by glucose-utilized cells in brain cells, and the distribution of phosphorylated glucose molecules. Prior to the decay of carbon 11-2-deoxy-D-glucose, the metabolic decomposition or utilization of carbon 11-2-deoxy-D-glucose is inhibited by the hypoxia of the 2' position in its molecular structure. Radioactive carbon 11 isotope through positron radiation The total decay is 11 atoms of boron, and the half-life of the carbon 11 atom decaying to boron 11 atom is about 20 minutes. After the carbon 11 isotope undergoes radioactive decay, it will be converted to boron 11 and no longer produce 511 KeV gamma rays.

Through the use of the above technical means, the present invention is a novel method for stimulating brain cognitive response image formation through visual, auditory and cognitive sensation, by detecting carbon 11-2-deoxy-D-glucose in the brain for glucose metabolism imaging. The direct information reflects the functional image of the brain in the face of visual, auditory and cognitive stimuli. This is the main purpose of the invention.

Example:

The drug was used: carbon-11-2-deoxy-D-glucose ([1-C-11]2-Deoxy-D-Glucose (hereinafter referred to as C-11-2DG)).

Pharmacy specification: Prepared as an isotonic nuclear-grade sterile injection, prepared by the automatic synthesis box of PET Center, with a radiochemical purity greater than 95%.

Test process (1):

1. The subject was fasted for 6 hours.

2. Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

3. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

4. After the injection, the subject rested in a quiet state for about 45 minutes, urinating and lying on the examination bed, and then performing a brain scan with PET imaging (for example, GE Discovery LS type). The image was subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 1 having C-11-2DG in the brain of the subject, and moving image 1 was used as a standard absorption value.

5. Subjects open their eyes and imagine the whole process under the control of visual stimulation conditions. A musical melody that is already well known.

6. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

7. Step 6: After the injection, the subject rests in a quiet state for about 45 minutes, urinates and lies on the examination bed, and then performs a brain scan with PET imaging (for example, GE Discovery LS type). The image is subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 2 with C-11-2DG in the subject's brain.

8. The moving image 2 was compared with the moving image 1 to observe changes in the C-11-2DG activity in the brain before and after the visual stimulation condition control.

Test process (2):

1. The subject was fasted for 6 hours.

2. Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

3. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

4. After the injection, the subject rested in a quiet state for about 45 minutes, urinating and lying on the examination bed, and then performing a brain scan with PET imaging (for example, GE Discovery LS type). The image was subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 3 having C-11-2DG in the brain of the subject, and moving image 3 was used as a standard absorption value.

5. Under the control of auditory stimulation conditions, the subject opens his eyes and listens to the music melody he is already familiar with.

6. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

7. After the injection, the subject rested in a quiet state for about 45 minutes, urinating and lying on the examination bed, and then performing a brain scan with PET imaging (for example, GE Discovery LS type). The image is subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 4 having C-11-2DG in the subject's brain.

8. The moving image 4 is compared with the moving image 3 to observe changes in the C-11-2DG activity in the brain before and after the subject is controlled by the auditory stimulation condition.

Test procedure (3):

1. The subject was fasted for 6 hours.

2. Under the control of closed eye conditions, the subject did not think about any problems and did not imagine anything.

3. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

4. After the injection, the subject rested in a quiet state for about 45 minutes, urinating and lying on the examination bed, and then performing a brain scan with PET imaging (for example, GE Discovery LS type). The image was subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 5 having C-11-2DG in the brain of the subject, and the moving image 5 was used as a standard absorption value.

5. Subjects under the control of cognitive stimulation conditions, open the eyes all the way and imagine the hand playing the piano, the finger playing with the beat action itself is already familiar with the music melody.

6. Subjects were given intravenous C-11-2DG at a dose of 16-20 mCi.

7. After the injection, the subject rested in a quiet state for about 45 minutes, urinating and lying on the examination bed, and then performing a brain scan with PET imaging (for example, GE Discovery LS type). The image is subjected to attenuation-corrected image processing for image reconstruction, showing a moving image 6 having C-11-2DG in the subject's brain.

8. The moving image 6 was compared with the moving image 5 to observe changes in C-11-2DG activity in the brain before and after control of cognitive stimulation conditions.

Referring to Figure 1, it is the first to sixth moving images (a), (b), (c), (d), (e), (f) of the three test procedures, which show three The metabolic amplitude of C-11-2DG in the brain changed before and after the stimulation condition. Among the three test results, the position of C-11-2DG in the brain after stimulation was similar to that in the brain, but the metabolism of C-11-2DG increased significantly.

Under the control of visual stimulation conditions, the glucose metabolism in the brain and prefrontal regions of the brain increased significantly, as shown in the visual (Visual) section of Figure 1, the difference in PET images before the test. However, in the traditional way of prior art such as fMRI, only the block with increased blood flow velocity can be proved, and it is not proved and presented by direct evidence.

Under the control of auditory stimulation conditions, the glucose metabolism in the brain blocks on both sides of the brain is significantly increased, as shown in the Auditory (Auditory) section of Fig. 1 by the difference in PET images before the test. However, in the traditional way of prior art such as fMRI, only the block with increased blood flow velocity can be proved, and it is not proved and presented by direct evidence.

Under the control of cognitive conditions, the glucose metabolism in the brain block of the brain increased significantly, but the prefrontal block did not increase significantly, as shown in the Cognitive part of Figure 1, the difference between the PET images before the test. . However, in the traditional way of prior art such as fMRI, only the block with increased blood flow velocity can be proved, and it is not proved and presented by direct evidence.

The above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Although the present invention has been disclosed in the above preferred embodiments, it is not intended to limit the present invention. A person skilled in the art can make some modifications or modifications to equivalent embodiments by using the above-disclosed technical contents without departing from the technical scope of the present invention. Technical Substantials Any simple modifications, equivalent variations, and modifications made to the above embodiments are still within the skill of the present invention. Within the scope of the program.

(a) ‧ ‧ first activity image

(b) ‧ ‧ second activity image

(c) ‧ ‧ third activity image

(d) ‧ ‧ fourth event image

(e) ‧ ‧ fifth event image

(f) ‧ ‧ sixth activity image

Claims (10)

A method for stimulating brain cognitive response image formation, comprising the following steps: Step S11: Subject under the control of closed eye condition, no problem is considered in the whole process, and nothing is imagined; Step S12: injecting the subject a glucose molecule having a carbon 11 isotope; step S13: performing brain imaging on the subject, and displaying the first activity map of the glucose molecule having the carbon 11 isotope in the brain of the subject in the display unit of the contrast apparatus For example, the first moving image is used as a standard absorption value; step S14: the subject opens the eyes and imagines the familiar music melody under the control of the visual stimulation condition; Step S15: injecting the subject with the carbon 11 isotope Glucose molecule; step S16: performing brain imaging on the subject, and displaying the second active image of the glucose molecule having the carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument; S17: comparing the second moving image with the first moving image, observing the subject having a carbon 11 co-location in the brain before and after the visual stimulation condition control The change in the molecular activity of the glucose; wherein the second moving image presents a moving image of the glucose molecule having a carbon 11 isotope in the brain when the subject opens both eyes and imagines a musical melody. The method of stimulating brain cognitive response image formation according to claim 1, wherein the glucose molecule having a carbon 11 isotope is carbon 11-2-deoxy-D-glucose. The method for stimulating brain cognitive response image formation according to claim 1, wherein the visual stimulation condition is a test room of a single soft color system, or a test room of a different color system, or a test room is provided with a music related symbol. Form with musical instrument image The method of stimulating brain cognitive response image formation according to claim 1, wherein the technique of brain imaging is positron angiography or single photon angiography. A method for stimulating brain cognitive response image formation includes the following steps: Step S21: The subject does not think about any problem and does not imagine anything under the control of the closed eye condition; Step S22: Injecting the subject a glucose molecule having a carbon 11 isotope; step S23: performing brain imaging on the subject, and displaying a third activity map of the glucose molecule having the carbon 11 isotope in the brain of the subject in a display unit of the contrast apparatus For example, the third moving image is used as a standard absorption value; step S24: the subject opens the eyes and listens to the music melody under the control of the auditory stimulation condition; Step S25: injecting the subject with glucose having a carbon 11 isotope Molecule; step S26: performing brain imaging on the subject, and displaying a fourth moving image of the glucose molecule having carbon 11 isotope in the brain of the subject in the display unit of the contrast instrument; step S27: The fourth moving image is compared with the third moving image, and the subject is observed to have a carbon 11 isotope grape in the brain before and after the auditory stimulation condition is controlled. A change in the activity of the sugar molecule; wherein the fourth activity image shows a moving image of the glucose molecule having a carbon 11 isotope in the brain when the subject opens both eyes and listens to the music melody. The method of stimulating brain cognitive response image formation according to claim 5, wherein the glucose molecule having a carbon 11 isotope is carbon 11-2-deoxy-D-glucose. The method for stimulating brain cognitive response image formation according to claim 5, wherein the auditory stimulation condition is, but not limited to, a form of playing music in the form of a CD or the like, and playing in the field. A method for stimulating brain cognitive response image formation includes the following steps: Step S31: The subject does not think about any problem during the whole process of closed eye condition control, and does not imagine anything; Step S32: Injecting the subject a glucose molecule having a carbon 11 isotope; step S33: performing brain imaging on the subject, and displaying a fifth activity diagram of the glucose molecule having a carbon 11 isotope in the brain of the subject in a display unit of the contrast apparatus Like, the fifth The moving image is used as a standard absorption value; step S34: the subject opens the eyes under the control of the cognitive stimulation condition and imagines playing the piano with the hand, the finger playing the music melody with the beat action; step S35: injecting the subject a glucose molecule having a carbon 11 isotope; step S36: performing brain imaging on the subject, and displaying a sixth activity map of the glucose molecule having the carbon 11 isotope in the brain of the subject in a display unit of the contrast apparatus Step S37: comparing the sixth active image with the fifth active image, observing changes in glucose activity of the carbon 11 isotope in the brain before and after control of the cognitive stimulation condition; The sixth moving image shows a moving image of a glucose molecule having a carbon 11 isotope in the brain when the subject opens his eyes and imagines playing the piano with the hand and playing the music melody with the finger. The method of stimulating brain cognitive response image formation according to claim 8, wherein the glucose molecule having a carbon 11 isotope is carbon 11-2-deoxy-D-glucose. The method for stimulating brain cognitive response image formation according to claim 8, wherein the cognitive stimulation condition is, but not limited to, the subject playing in an imaginary form with his own hands in the test room, or In the form of melody, playing the soft music melody familiar and loved by the subject.