201118364 六、發明說明： 【發明所屬之技術領域】 本發明是有關於一種對溶液試料的旋光度進行測定， 並求出溶質的比旋光度以及濃度的旋光計。 【先前技術】 旋光性是使入射的直線偏光的偏光面旋轉的物質的性 質，將直線偏光入射至具有旋光性的物質時，偏光面所旋 轉的角度稱作旋光度。當試料為溶液時，將溶液的旋光度 除以直線偏光透過溶液的長度以及溶質的濃度所得的值稱 作比旋光度，比旋光度是物質固有的值。當存在比旋光度 未知的物質時，可將該物質作為溶質而製作特定濃度的溶 液，對所製作的溶液的旋光度進行測定，並根據濃度與所 測定出的旋光度來求出物質的比旋光度。相反地，當存在 比·光度為已知的物質成為溶質的溶液時’可對溶液的旋 光度進行測定’並根據已知的旋光度與所測定出的旋光度 來求出溶液中的物質的濃度。 對溶液試料的旋光度進行測定的旋光計，藉由使來自 光源的光通過偏光元件而產生直線偏光，使直線偏光入射 至溶液試料，並使透過溶液試料的直線偏光入射至檢光元 件，以對透過檢光元件的光量進行檢測。一方面對透過檢 光元件的光量進行檢測，一方面使檢光元件旋轉，求出透 過才欢光元件的光里成為令的檢光元件的旋轉角度，藉此可 求出直線偏光透過/谷液S式料時偏光面所旋轉的角度，即求 出溶液試料的旋光度。 201118364 先前’作為旋光計的光源，多使用鈉燈（ natrium lamp)。將鈉燈作為光源的旋光計中，使用鈉的j)線的光 (波長=約589.3 run)來進行旋光度的測定。近年來，考 慮到光源的低成本（cost)化或小型化等的觀點，也開發 出使用成氣燈（xenon lamp)或發光二極體（light-emitting diode ’ LED)等鈉燈以外的光源的旋光計。專利文獻1中 揭示有一種可利用多種波長來測定旋光度的旋光計。 [先行技術文獻] •[專利文獻] [專利文獻1]日本專利第4057707號公報 然而’物質的旋光度存在波長依存性，當用於測定旋 光度的光的波長有所變化時，所測定出的旋光度以及比旋 光度的值亦會發生變化。使用LED等的鈉燈以外的光源的 旋光计中，與對光源使用鈉燈的先前的旋光計相比，用於 測定旋光度的光的波長有所不同，因此所測定出的旋光度 以及比旋光度的值亦不同。文獻中記載的值等過去所測定 # 出的比旋光度的值，多為使用鈉的D線的光而測定出的 值’因而存在下述問題，gP，無法對使用$同波長的光而 新測定出的比旋光度的值與過去所測定出的比旋光度的值 進行單純比較。 而且’使用LED等納燈以外的光源的旋光計中，難以 統一用於測定的光的波長。例如，當使用LED來作為光源 時，旋光計藉由進一步使用干涉濾光器（mter)來限定來 自LED的光的波長’從而生成單色的光。LED的發光波 5 201118364 長與干涉濾光器所限定的光的波長對於每個零件而言存在 偏差，因此用於測定的光的波長於各旋光計中亦存在偏 差。因而，即使測定相同物質的比旋光度，若旋光計不同， 則測定出的比旋光度的值亦會稍許不同，從而存在無法嚴 格地比較所測定出的比旋光度的問題。對光源使用鈉燈的 旋光计中，用於測定的鈉的D線是鈉原子的明線光譜 ^spectrum)，因此波長嚴格固定，不會引起因波長引起的 旋光度的測定結果的偏差。如此，測定出的比旋光度的值 於各奴光3十中不同的問題是使用LED等鈉燈以外的光源 的旋光計所特有的問題。 【發明内容】 “本發明疋有蓉於此情況而完成，其目的在於提供一種 旋光計，將由旋光計所測定出的旋光度轉換成使用特定波 長=光來進行測定時應得的旋光度，藉此，可進行過去所 測疋的比旋光度以及由其他旋光計所測定的比旋光度與所 測定出的比旋光度的比較。 ' 本發明的旋光計具備產生單色的直線偏光的直線偏光 產生機構，對與透過試料的直線偏光的偏光面所旋轉的角 度對應的試料的旋光度進行測定，此旋光計的特徵在於包 括將依存於上述直線偏光產生機構所產生的直線偏光的 =長與特定波長的係數乘以所測定出的旋光度，藉此來將 旋光度轉換為上述特定波長下賴光度的機構。 於本發明中，使用單色的直線偏光來測定試料的旋光 度的旋光計對婦職林進_定’錄顧光度的波 201118364 長依，性，將所測定出的旋光度轉換成特定波長下的旋光 度。藉由對旋光度進行轉換，可對因旋光計的不同造成的 方疋光度的值的偏差進行修正。 本發明的旋光計更包括：當使用溶液試料來作為試料 時，接收ί谷液试料的溶質的濃度的值的機構；使用所接收 的溶質的濃度的值，根據轉換後的旋光度來計算溶質的比 旋光度的機構；以及輸出所計算出的比旋光度的值的機構。 而且，於本發明中，當試料為溶液試料，且溶液試料 的溶質的濃度為已知時，旋光計接收濃度的值，並根據轉 換後的旋光度與溶液試料的溶質的濃度來計算溶質的比旋 光度。由於是根據轉換後的旋光度來計算比旋光度，因此 不會產生因旋光計的不同造成的比旋光度的值的偏差。 本發明的旋光計更包括··當使用溶液試料來作為試料 日守’接收溶液試料的溶質的比旋光度的值的機構；使用所 接收的溶質的比旋光度的值’根據轉換後的旋光度來計算 溶質的濃度的機構；以及輸出所計算出的濃度的值的機構。 • 而且，於本發明中，當溶液試料的溶質的比旋光度為 已知時，旋光計接收比旋光度的值，並根據轉換後的旋光 度與溶液試料的溶質的比旋光度來計算溶液試料中的溶質 的濃度。由於是根據轉換後的旋光度來計算濃度，因此不 會產生因旋光計的不同造成的溶質的濃度的值的偏差。 本發明的旋光計中，上述直線偏光產生機構是包含發 光二極體以及偏光板而構成。 而且，於本發明中，旋光計使用led來作為用於產生 201118364 旋光度的測定時所用的單色的直線偏光的光源。 本發明的旋光計中，上述特定波長是鈉的0線的波長。 而且，於本發明中，旋光計在將測定出的旋光度轉換 成使用特定波長的直線偏光來進行測定時所得的旋光度 時，將先前的旋光計中多用作光源的鈉的D線的波長作為 特定波長來對旋光度進行轉換。 本發明的旋光計更包括：檢光元件，入射有直線偏光； 轉動機構，使該檢光元件轉動；受光機構，接收透過上述 檢光元件的光；以及角度顯示部，使用對應於上述檢光元 件在上述轉動機構的作用下的轉動而轉動的圖形來顯示上 述檢光元件的轉動角度。 進而，於本發明中’旋光計具備顯示對應於檢光元件 的轉動而轉動的圖形的角度顯示部，通過角度顯示部的圖 形的轉動’來顯示旋光度的測定中逐次變化的檢光元件的 轉動角度。 (發明的效果） 於本發明中，測定出的旋光度的值會根據旋光計而有 所偏差’但藉由將測定出的旋光度轉換成特定波長下的旋 光度’可對因旋光計的不同造成的旋光度的值的偏差進行 修正。本發明中，由於是根據轉換後的旋光度來計算溶液 試料的溶質的比旋光度，因此不會產生因旋光計的不同所 造成的比旋光度的值的偏差，從而可實現所測量的比旋光 度與由其他旋光計所測量的比旋光度的單純比較。 而且’於本發明中，由於是根據轉換後的旋光度來計 201118364 算浴液试料的溶質的濃度，因此不會產生因旋光計的不同 所造成的遭度的值的偏差，從而可實現所測量的濃度與由 其他旋光計所測量的濃度的單純比較。 、進而’於本發明中’藉由將先前的旋光計中多用作光 $的鈉的D_波長作為特定波長來雜光度進行轉換， 從而可對與由使用鈉燈來作為光源的先前的旋光計所測定 出的比旋光度相當的比旋光度進行測量。因而，可對文獻 巾1的值等彻對絲使賴㈣旋光計而在過去測定 的比方疋光度的值、與利用本發明的旋光計戶斤測量的比旋光 度的值進行單純比較。 進，’於本發明中’在利用旋光計來測定旋光度的過 ^中i藉由—邊轉動一邊表示角度的箭頭等的圖形來表示 仏光元件的轉動角度，因此使用者能夠由圖形的轉動而直 ^地瞭解測定中的檢光元件的轉動角度。而且，本發明可 ^揮下述等優異效果，即，使用者通過觀察逐次變化的檢 光元件的轉動角度’能夠在旋光度的測定結束之前直觀地 • 推測出旋光度是多少左右的值。 ▲為讓本發明之上述和其他目的、特徵和優點能更明顯 易懂，下文特舉較佳實施例，並配合所附圖式，作詳細 明如下。 【實施方式】 以下，根據表示實施形態的圖式來具體說明本發明。 ，1是表示本發明的旋光計的内部結構的結構圖。圖 中的箭頭為光路，旋光計是將光源3卜干涉遽光器32、透 201118364 鏡（lens) 33、偏光元件11、樣本盒（sampie ceH) η、法 拉第線圈（Faradaycoil) 13、中空馬達（m〇t〇r) 14、檢光 元件15、透鏡34、受光器件16沿著光路排列而構成。光 源31是發出單色光的LED，且連接於點燈電路3〇。光源 31從點燈電路30受到點燈用的電力供給而發光。再者， 光源31亦可為氙氣燈等LED以外的光源。干涉濾光器32 是使用於旋光度測定的波長的光通過，而阻斷其他波長的 光的帶通（bandpass)光學濾波器。 偏光兀件11是僅使與單一的透過軸平行的直線偏光 成分透過的偏光板，將光源31發出後通過干涉濾光器32 以及透鏡33而入射的光轉換成直線偏光。藉此，產生直線 偏光。偏光兀件11被固定於旋光計内，偏光元件u所固 有的透過軸的方向亦被固定，因此由偏光元件u所產生的 偏光的偏光面為固定。藉由光源31、點燈電路3〇、透鏡 以及偏光元件11 ’構成本發明中的直線偏光產生機構。 藉由光源31的發光波長以及通過干涉濾光器32的光的波 長’用於旋光度測定的光的波長得以確定，藉由偏光元件 U ’直線偏光的偏光面得以確定。 樣本盒12疋注入溶液試料的透明盒，配置於光路穿過 溶液試料内的位置上。法拉第線圈13配置於光路穿過内部 的位置上，且連接於產生交流電流的振盪器22。振盪器22 將規定頻率的交流電流供給至法拉第線圈i3，法拉第線圈 13藉由受到交流電流的供給而使内部產生振動磁場 。通過 法拉弟線圈13的直線偏光在振動磁場的作用下偏光面以 201118364 =流電流相應的振幅以及頻率而擺動振動。再者，法拉 第線圈13與樣本盒12的排列順序亦可相反。201118364 VI. Description of the Invention: [Technical Field] The present invention relates to a polarimeter that measures the optical rotation of a solution sample and determines the specific optical rotation and concentration of the solute. [Prior Art] Optical rotation is a property of a substance that rotates a polarizing surface of an incident linearly polarized light. When a linearly polarized light is incident on an optically active substance, the angle at which the polarizing surface rotates is called an optical rotation. When the sample is a solution, the value obtained by dividing the optical rotation of the solution by the length of the linearly polarized light transmissive solution and the concentration of the solute is referred to as the specific optical rotation, and the specific optical rotation is a value inherent to the substance. When there is a substance having an unknown degree of optical rotation, the substance can be used as a solute to prepare a solution having a specific concentration, and the optical rotation of the produced solution can be measured, and the ratio of the substance can be determined according to the concentration and the measured optical rotation. Optical rotation. Conversely, when there is a solution in which a substance having a known luminosity is a solute, 'the optical rotation of the solution can be measured' and the substance in the solution can be determined from the known optical rotation and the measured optical rotation. concentration. A polarimeter for measuring the optical rotation of a solution sample is obtained by passing light from a light source through a polarizing element to generate linearly polarized light, causing linearly polarized light to be incident on a solution sample, and linearly polarized light that has passed through the solution sample is incident on the light detecting element, The amount of light transmitted through the light detecting element is detected. On the other hand, the amount of light passing through the light detecting element is detected, and on the other hand, the light detecting element is rotated, and the rotation angle of the light detecting element which is transmitted through the light of the light emitting element is obtained, thereby obtaining the linear polarized light transmission/grain The angle at which the polarizing surface rotates during the S-type material is the optical rotation of the solution sample. 201118364 Previously used as a light source for polarimeter, a natrium lamp was used. In the polarimeter using a sodium lamp as a light source, the optical rotation of the j) line of sodium (wavelength = about 589.3 run) was used to measure the optical rotation. In recent years, in consideration of cost reduction and miniaturization of a light source, a light source other than a sodium lamp such as a xenon lamp or a light-emitting diode (LED) has been developed. Polarimeter. Patent Document 1 discloses a polarimeter that can measure optical rotation using a plurality of wavelengths. [PRIOR ART DOCUMENT] [Patent Document 1] Japanese Patent No. 4057707. However, the optical rotation of a substance has a wavelength dependency, and when the wavelength of light for measuring the optical rotation changes, the measurement is performed. The optical rotation and the value of the specific optical rotation also change. In a polarimeter using a light source other than a sodium lamp such as an LED, the wavelength of light for measuring the optical rotation differs from that of the previous polarimeter using a sodium lamp for the light source, and thus the measured optical rotation and specific optical rotation The value is also different. The values described in the literature, such as the value of the specific optical rotation measured in the past, are mostly the values measured using the light of the D line of sodium. Therefore, there is a problem that gP cannot be used for the light of the same wavelength. The value of the newly measured specific optical rotation is simply compared with the value of the specific optical rotation measured in the past. Further, in a polarimeter using a light source other than a nano lamp such as an LED, it is difficult to unify the wavelength of light used for measurement. For example, when an LED is used as the light source, the polarimeter generates monochromatic light by further using an interference filter to define the wavelength of light from the LED. Luminous wave of LED 5 201118364 The wavelength of light defined by the length and interference filter varies for each part, so the wavelength of the light used for measurement is also deviated in each polarimeter. Therefore, even if the specific optical rotation of the same substance is measured, if the polarimeter is different, the value of the measured specific optical rotation is slightly different, and there is a problem that the measured specific optical rotation cannot be strictly compared. In a polarimeter using a sodium lamp for a light source, the D line of sodium used for measurement is a bright line spectrum of sodium atoms, so the wavelength is strictly fixed, and variations in measurement results of optical rotation due to wavelength are not caused. As described above, the value of the measured specific optical rotation differs from that of the slave light, which is a problem unique to a polarimeter using a light source other than a sodium lamp such as an LED. SUMMARY OF THE INVENTION "The present invention has been completed in view of the above circumstances, and an object thereof is to provide a polarimeter that converts the optical rotation measured by a polarimeter into an optical rotation which is obtained when a specific wavelength = light is used for measurement. Thereby, the specific optical rotation measured in the past and the specific optical rotation measured by another polarimeter can be compared with the measured specific optical rotation. The polarimeter of the present invention has a straight line which produces a linear linear polarization. The polarization generating means measures the optical rotation of the sample corresponding to the angle of rotation of the polarized surface of the linearly polarized light passing through the sample, and the polarimeter is characterized by including a linearly polarized light generated by the linearly polarized light generating means. A mechanism for converting the optical rotation to the illuminance at the specific wavelength by multiplying the coefficient of the specific wavelength by the measured optical rotation. In the present invention, the optical rotation of the sample is measured by using a linear linear polarization of the sample.算对妇林进_定' Recording the illuminance of the wave 201118364 Long, sex, the measured optical rotation into optical rotation at a specific wavelength By converting the optical rotation, the deviation of the value of the square luminosity caused by the difference of the polarimeter can be corrected. The polarimeter of the present invention further includes: when using the solution sample as a sample, receiving the liquid solution a mechanism for the value of the concentration of the solute of the material; a mechanism for calculating the specific optical rotation of the solute based on the converted optical illuminance using the value of the concentration of the received solute; and a mechanism for outputting the calculated value of the specific optical rotation. Moreover, in the present invention, when the sample is a solution sample, and the concentration of the solute of the solution sample is known, the polarimeter receives the value of the concentration, and calculates the solute according to the converted optical rotation and the concentration of the solute of the solution sample. Since the specific optical rotation is calculated based on the converted optical rotation, the deviation of the specific optical rotation due to the difference in the polarimeter does not occur. The polarimeter of the present invention further includes a solution sample. The mechanism for taking the value of the specific optical rotation of the solute receiving the solution sample as the sample; the value of the specific optical rotation using the received solute is based on the converted a mechanism for calculating the concentration of the solute; and a mechanism for outputting the value of the calculated concentration. Moreover, in the present invention, when the specific optical rotation of the solute of the solution sample is known, the polarimeter receives the specific optical rotation. The value, and the concentration of the solute in the solution sample is calculated based on the ratio of the optical rotation after conversion to the solute of the solution sample. Since the concentration is calculated based on the converted optical rotation, there is no difference due to the difference in the polarimeter. In the polarimeter of the present invention, the linearly polarized light generating means includes a light emitting diode and a polarizing plate. Further, in the present invention, the polarimeter uses LED as a method for generating 201118364. A monochromatic linearly polarized light source used for the measurement of optical rotation. In the polarimeter of the present invention, the specific wavelength is a wavelength of 0 linear line of sodium. Further, in the present invention, the optical rotation meter converts the measured optical rotation. When the optical rotation obtained by measurement is performed using linear polarized light of a specific wavelength, the D-line of sodium which is often used as a light source in the previous polarimeter is used. Long to convert a rotation of a specific wavelength. The polarimeter of the present invention further includes: a light detecting element that is incident with linearly polarized light; a rotating mechanism that rotates the light detecting element; a light receiving mechanism that receives light transmitted through the light detecting element; and an angle display portion that uses the light detecting corresponding to the light detecting unit A pattern in which the element is rotated by the rotation of the rotating mechanism to display the angle of rotation of the light detecting element. Further, in the present invention, the 'the polarimeter includes an angle display unit that displays a pattern that rotates in accordance with the rotation of the light detecting element, and displays the light detecting element that changes successively in the measurement of the optical rotation by the rotation of the pattern of the angle display unit. The angle of rotation. (Effects of the Invention) In the present invention, the value of the measured optical rotation is deviated according to the polarimeter 'but the optical rotation at a specific wavelength can be converted by the optical rotation of the specific wavelength. The deviation of the values of the different optical rotations is corrected. In the present invention, since the specific optical rotation of the solute of the solution sample is calculated based on the converted optical rotation, the deviation of the specific optical rotation due to the difference in the polarimeter does not occur, so that the measured ratio can be realized. A simple comparison of the optical rotation with the specific optical rotation measured by other polarimeters. Further, in the present invention, since the concentration of the solute of the 201118364 bath sample is calculated based on the converted optical rotation, the deviation of the value due to the difference in the polarimeter does not occur, thereby realizing A simple comparison of the measured concentration with the concentration measured by other polarimeters. And in the present invention, by converting the D_wavelength of the sodium used as the light of the previous optical illuminator as a specific wavelength, the luminosity can be converted to a previous polarimeter using a sodium lamp as a light source. The specific optical rotation measured by the specific optical rotation was measured. Therefore, the value of the document towel 1 can be simply compared with the value of the specific illuminance measured in the past by the polarimeter, and the value of the specific rotation measured by the rotatory meter of the present invention. In the present invention, in the case where the optical rotation is measured by a polarimeter, i indicates the angle of rotation of the calender element by a pattern such as an arrow indicating an angle of rotation, so that the user can be graphically Rotate and directly understand the angle of rotation of the photodetecting element under measurement. Further, the present invention can provide an excellent effect that the user can visually estimate the value of the optical rotation degree before the end of the measurement of the optical rotation degree by observing the rotation angle of the light-detecting element which is successively changed. The above and other objects, features, and advantages of the present invention will become more apparent from the aspects of the invention. [Embodiment] Hereinafter, the present invention will be specifically described based on the drawings showing embodiments. 1 is a structural view showing the internal structure of the polarimeter of the present invention. The arrow in the figure is the optical path, and the polarimeter is the light source 3 interfering with the chopper 32, through the 201118364 lens 33, the polarizing element 11, the sample box (sampie ceH) η, the Faraday coil (Faradaycoil) 13, the hollow motor ( M〇t〇r) 14. The light detecting element 15, the lens 34, and the light receiving device 16 are arranged along the optical path. The light source 31 is an LED that emits monochromatic light and is connected to the lighting circuit 3A. The light source 31 is supplied with electric power for lighting from the lighting circuit 30 to emit light. Further, the light source 31 may be a light source other than an LED such as a xenon lamp. The interference filter 32 is a bandpass optical filter that blocks light of a wavelength measured by optical rotation and blocks light of other wavelengths. The polarizing element 11 is a polarizing plate that transmits only a linearly polarized light component parallel to a single transmission axis, and converts the light that is emitted by the light source 31 and then incident through the interference filter 32 and the lens 33 into linearly polarized light. Thereby, linear polarization is generated. The polarizing element 11 is fixed in the polarimeter, and the direction of the transmission axis fixed by the polarizing element u is also fixed. Therefore, the polarizing surface of the polarized light generated by the polarizing element u is fixed. The linear light-polarizing generating mechanism in the present invention is constituted by the light source 31, the lighting circuit 3, the lens, and the polarizing element 11'. The wavelength of the light used for the optical rotation measurement by the wavelength of the light emitted from the light source 31 and the wavelength of the light passing through the interference filter 32 is determined, and the polarizing surface which is linearly polarized by the polarizing element U' is determined. The sample box 12 is inserted into a transparent box of the solution sample, and is disposed at a position where the optical path passes through the solution sample. The Faraday coil 13 is disposed at a position where the optical path passes inside, and is connected to the oscillator 22 that generates an alternating current. The oscillator 22 supplies an alternating current of a predetermined frequency to the Faraday coil i3, and the Faraday coil 13 generates a vibrating magnetic field internally by the supply of an alternating current. The linearly polarized light passing through the Faraday coil 13 is oscillated by the vibrational magnetic field under the action of the vibrational magnetic field at the amplitude and frequency of the current of 201118364 = current. Further, the arrangement order of the Faraday coil 13 and the sample cartridge 12 may be reversed.
中空馬達14是形成為中空筒狀的電動馬達，且配置於 光路穿過Μ部分的位置上。於中空馬達㈣轉子上，於 堵塞中空馬達14的開口部的位置處固定著檢光元件15。 檢光70件15是具有單—的透過軸的偏光板。通過樣本盒 12以及法拉第軸13後的直線偏光通過巾空馬達14的中 工部为，亚人射至檢光元件15。人射至檢光元件15的直 線偏光中，僅與透過軸平行的直線偏光成分透過檢光元件 15。而且，中空馬達14連接於馬達驅動器（则如—㈦ 23,而形絲給來自馬達鶴器23的鶴電⑽使轉子轉 動的構成。藉由轉動中空科14的轉？，使岐於轉子上 的檢光元件15轉動。藉由轉動檢光元件15，使檢光元件 15所固有的透過㈣方向發生變化，且透過檢光元件 的直線偏光的強度發生變化。 透過檢光το件15的直線偏光經過透鏡34後入射至受 光器件16。又光益件16是由光二極體（咖t〇 di〇de)等 構成’接收直線偏光m壓來表示受光量的受光信 號輸出至放大部24。受光器件16所輸出的受光信號的強 度疋與受光器件16所接收的受光量對應。 本电明的旋光计更具備用於進行信號處理的信號處理 部21 ’該信號處理是•根據受絲件16所輸出的受光 信號來控制旋光計的動作。於信號處理部21上，連接有振 盪器22、馬達驅動器23以及放大部24,放大部24對受光 11 201118364 輸出的受光信親行放大後輪人至信號處理部 = 部21輸出用於使振盪器22以及馬達驅動哭 ㈣信號。中空馬達14以及馬達驅動器23 _ =，:明中的轉動機構。信號處理部21是包括用於輸出又 二唬的輸出入介面（I/〇 interface)、執行各種運算處 理的^處理器（micropr〇cess〇r)或積體電路等的運算部、 及對信號處理所需的臨時資訊進行記憶的記憶體 (memory)而構成。而且，於信號處理部21上，連接有 顯二旋光度的測定結果等的資訊賴示部4、藉由使用者 的操作來接鍵光度的财開始等的各種Μ的操作部 25、及對信號處理所需的處理程式（pr〇gram)以及資料 (data)進行記憶的非揮發性的記憶部％。信號處理部u 依據記憶冑26所記憶的處理程式，來進行用於控制各部分 的動作的信號處理。 而且’記憶部2 6記憶有用於旋光度測定的直線偏光的 波長的值、與直線偏技過縣盒12巾所注人的溶液試料 :的長度的值。用於旋光度測定的直線偏光的波長的值是 藉由光，31的發光波*以及通過干频光^ %的光料 · 長來確疋。用於旋光度測定的直線偏光的波長的值是在製 造旋光計時進行㈣，麵實騎得的值記憶至記憶部％ 中。直線偏光透過溶液試料中的長度的值是沿著樣本盒12 的注入溶液試料的部分的光路的大小，且由樣本盒12的大 小規格來確定。於記憶部26巾，預先記憶有由規格所確定 的值。進而，於記憶部26中，記憶有後述的對旋光度進行 12 201118364 轉換的數式所需的參數（parameter )的值。 於旋光度的測定開始前的階段，將檢光元件15 位置狀在偏光元件u以及檢光元件15的透過軸正3 =始轉，位置。偏光元件u以及檢光元件15的透過轴正 父=狀悲稱作正交偏光（cr〇ss nic〇ls)狀態。在正交偏光 狀態下’若樣本盒n内無溶液試料，則入射至檢光元件 15的直線偏光的偏光面與檢光元件15的透過軸正交，因 此光全部被檢光元件15所遮蔽，而受光器件16盏法接收 • 光。 當樣本盒12内注入有具有旋光性的溶液試料時，直線 偏光的偏光面因溶液試料而旋轉，與檢光元件15的透過轴 平行的直線偏光成分透過檢光元件15，受光器件16對光 進行檢測。信號處理部21進行輸出用於使振盪器22產生 父流電流的控制信號的處理’振盪器22將規定頻率f的交 流電流供給至法拉第線圈13。法拉第線圈13藉由受到規 定頻率f的交流電流的供給而產生以頻率f來振動的振動 φ 磁場。通過法拉第線圈丨3的直線偏光在振動磁場的作用 下’偏光面以頻率f而擺動振動。此時，直線偏光的偏光 面以與法拉第線圈13所產生的振動磁場的振幅相應的振 動角寬度來進行頻率f的擺動振動。 圖2A、圖2B以及圖2C是表示直線偏光的偏光面的 變化的概念圖。圖中所示的箭頭表示與直線偏光的偏光面 平行且與行進方向正交的偏光方向。而且，角度0的方向 是偏光元件Π的透過軸的方向，角度90。的方向是配置於 13 201118364 初始轉動位置上的檢光元件15的透過軸的方向。圖2A表 示透過偏光元件11後的直線偏光的偏光面，偏光方向是與 檢光元件15的透過轴正交。圖2B表示透過樣本盒12中 的溶液試料後的直線偏光的偏光面。直線偏光的偏光面因 溶液試料的旋光性而旋轉，偏光面與角度〇的方向所成的 角度為溶液試料的旋光度α。圖2C表示進而通過法拉第線 圈13後的直線偏光的偏光面。偏光面進行與角度〇的方向 所成的角度以角度α為中心而在振動角寬度δ内週期性變 動的擺動振動。圖2C中表示α>δ的示例。 信號處理部21進行將用於使中空馬達14轉動的脈波 (pulse)信號輸出至馬達驅動器23的處理。馬達驅動器 23將與來自信號處理部21的脈波信號相應的驅動電流^ 給至中空馬達14,中空馬達14使檢光元件15轉動。^由' 信號處理部21所輸出的脈波信號的種類，中空馬達^的 轉動方向得以確定，而且，藉由脈波信號的數量，轉動角 度得以確定。中空馬達14使檢光元件15在與來自信號斤 理部21的信號相應的方向上轉動無波信號對應 角度，隨後停止。而且，信號處理部21進行下述處理， 根據所輸出的脈波信號的數量，對自正交偏光狀離 轉動位置開始使檢光元件15轉動的中空馬達Μ的轉勤° 度進行測量。藉由將轉子轉動1步的角度乘以直至轉動1 當前的轉動位置為止而輸出用於使中空馬達14 動1 =的脈波信號的數量，可對中空馬達14的轉動角 行測量。 201118364 圖3是表不轉動的檢光元件15的透過軸與直線偏光的 偏光面的關係的概念圖。將藉由中空馬達14而自初始轉動 位置開始轉動的檢光元件15的轉動角度設為p。於圖中， 示出轉動後的檢光元件15的透過軸，角度9〇。的方向與轉 動後的檢光元件15的透過軸所成的角為轉動角度p。而 且，於圖中，以虛線來表示與檢光元件15的透過轴正交的 方向。藉由直線偏光的偏光面進行擺動振動，入射至檢光 元件15的直線偏光的偏光面與檢光元件15的透過軸所成 φ ㈤角以頻率f而振動。此時的振動角寬度δ成為與法拉第 線圈13所產生的振動磁場的振幅相應的振動角寬度。入射 至轉動的檢光元件15的直線偏光中，僅與檢光元件15的 透過軸平行的直線偏光成分透過檢光元件15。透過檢光元 件15的光被受光器件16所接收。 接收到光的受光器件16輸出以電壓來表示受光量的 受光信號’放大部24對受光信號進行放大後輸人至信號處 理部21。如圖3所示，由於入射至檢光元件15的直線偏 的偏光面與檢光元件15的透過軸所㈣角是以頻率f 而振動’因此’直線偏光中所含的與檢光元件ls的透過轴 平行的直線偏光成分的大小亦以頻率f來振動。由於透過 檢光元件15的直線偏光成分的大小是以頻率f而振動，因 此接收到透過檢光元件15的光的受光器件16的受光量是 以頻率f來振動。_ ’以電壓來表示受光器件16中的受 光1的文光信號成為使電壓以頻率f而振動的交流信號。 直線偏光的偏光面與檢光元件15的透過軸所交叉的 15 201118364 2 直中角的透過檢光兀件15的直線偏光成分越小， 件15的透偏光㈣振動中心正 又榼動振動的偏光面與透過軸所交又的角度的範圍最接 近直角，因此受光㈣、“r ,又又的角度嶋圍敢接 處理部21自錢刻、。輸Μ料信號的信號 一*雷M 遷中提取以與供給至法拉第線圈13的 ^韓^ Τ f來"^的交流成分，並對中空馬達 仃饋（feedback)控制，以使提取出的交流 小。信號處理部21藉由反饋控制來規定 τf 技成分達到最小的檢光元The hollow motor 14 is an electric motor formed in a hollow cylindrical shape, and is disposed at a position where the optical path passes through the weir portion. The light detecting element 15 is fixed to the hollow motor (four) rotor at a position where the opening of the hollow motor 14 is blocked. The light detecting 70 member 15 is a polarizing plate having a single transmission axis. The linearly polarized light passing through the sample cartridge 12 and the Faraday axis 13 passes through the middle portion of the towel motor 14, and the submaniverts are incident on the light detecting element 15. In the linearly polarized light that the person has hit the light detecting element 15, only the linearly polarized light component parallel to the transmission axis passes through the light detecting element 15. Further, the hollow motor 14 is connected to a motor driver (for example, -(7) 23, and the wire is supplied to the crane (10) from the motor crane 23 to rotate the rotor. By rotating the hollow tube 14, the rotor is placed on the rotor. The light detecting element 15 rotates. By rotating the light detecting element 15, the transmission (four) direction inherent to the light detecting element 15 is changed, and the intensity of the linear polarized light transmitted through the light detecting element is changed. The polarized light is incident on the light-receiving device 16 after passing through the lens 34. The light-receiving member 16 is configured to output a light-receiving signal indicating the amount of received light to the amplifying portion 24 by a light diode (such as a photodiode). The intensity 疋 of the light receiving signal output by the light receiving device 16 corresponds to the amount of light received by the light receiving device 16. The polarimeter of the present invention further includes a signal processing unit 21 for performing signal processing. The operation of the polarimeter is controlled by the received light receiving signal of 16. The signal processing unit 21 is connected to the oscillator 22, the motor driver 23, and the amplifying unit 24, and the amplifying unit 24 receives the light receiving signal from the light receiving unit 11 201118364. The front-end amplifying rear-wheel human-to-signal processing unit=section 21 outputs a signal for driving the oscillator 22 and the motor to drive the crying (four). The hollow motor 14 and the motor driver 23 _ =, the turning mechanism of the light. The signal processing unit 21 includes It is used to output two input/output interfaces (I/〇 interface), a processor that performs various arithmetic processing (micropr〇cess〇r), or an integrated circuit, and temporary information required for signal processing. In addition, the signal processing unit 21 is connected to the information display unit 4 such as the measurement result of the second optical rotation, and the start of the luminosity by the user's operation. The operation unit 25 of the various types of devices, and the non-volatile memory unit % that memorizes the processing program (pr〇gram) and data required for signal processing. The signal processing unit u memorizes the memory 26 The program is used to perform signal processing for controlling the operation of each part. Further, the 'memory unit 26 stores the value of the wavelength of the linearly polarized light for the measurement of the optical rotation, and the solution of the person who is in the line of the straight line. Sample The value of the length of the linear polarized light used for the measurement of the optical rotation is determined by the light, the illuminating wave of 31, and the length of the light by the dry-frequency ray. The value of the wavelength of the linearly polarized light is obtained by performing the optical rotation timing (4), and the value of the surface riding is memorized to the memory portion %. The value of the length of the linearly polarized light transmitted through the solution sample is along the portion of the sample solution of the sample solution of the sample cartridge 12 The size of the optical path is determined by the size of the sample cartridge 12. In the memory unit 26, the value determined by the specification is stored in advance. Further, in the memory unit 26, the optical rotation degree described later is stored in the 12 201118364 conversion. The value of the parameter (parameter) required for the number. At the stage before the start of the measurement of the optical rotation, the position of the light-detecting element 15 is positively turned on and off at the position of the polarization axis of the polarizing element u and the light-detecting element 15. The polarizing element u and the transmission axis of the light detecting element 15 are called the orthogonal polarized light (cr〇ss nic〇ls) state. In the case of the orthogonal polarization state, if there is no solution sample in the sample cartridge n, the polarization plane of the linearly polarized light incident on the light detecting element 15 is orthogonal to the transmission axis of the light detecting element 15, and therefore all of the light is blocked by the light detecting element 15. And the light receiving device 16 cannot receive light. When a sample solution having optical activity is injected into the sample cartridge 12, the polarized surface of the linearly polarized light is rotated by the solution sample, and a linearly polarized component parallel to the transmission axis of the light detecting element 15 passes through the light detecting element 15, and the light receiving device 16 opposes light. Test. The signal processing unit 21 performs a process of outputting a control signal for causing the oscillator 22 to generate a parent current. The oscillator 22 supplies an AC current of a predetermined frequency f to the Faraday coil 13. The Faraday coil 13 generates a vibration φ magnetic field that vibrates at a frequency f by supplying an alternating current of a predetermined frequency f. The linearly polarized light passing through the Faraday coil 丨3 is oscillated and vibrated at a frequency f by the action of the vibration magnetic field. At this time, the linearly polarized polarization surface oscillates at a frequency f in accordance with the vibration angular width corresponding to the amplitude of the vibration magnetic field generated by the Faraday coil 13. 2A, 2B, and 2C are conceptual diagrams showing changes in the polarizing surface of the linearly polarized light. The arrows shown in the figure indicate polarization directions which are parallel to the polarizing surface of the linearly polarized light and orthogonal to the traveling direction. Further, the direction of the angle 0 is the direction of the transmission axis of the polarizing element ,, and the angle is 90. The direction is the direction of the transmission axis of the light detecting element 15 disposed at the initial rotational position of 13 201118364. Fig. 2A shows a polarization plane of linearly polarized light transmitted through the polarizing element 11, and the polarization direction is orthogonal to the transmission axis of the light detecting element 15. Fig. 2B shows the polarized surface of the linearly polarized light after passing through the sample in the sample cartridge 12. The polarized surface of the linearly polarized light is rotated by the optical rotation of the solution sample, and the angle formed by the direction of the polarized surface and the angle 〇 is the optical rotation α of the solution sample. Fig. 2C shows a polarizing surface of the linearly polarized light which is further passed through the Faraday coil 13. The polarizing surface performs a swinging vibration that periodically changes in the vibration angular width δ with the angle formed by the direction of the angle 〇 centered on the angle α. An example of α > δ is shown in Fig. 2C. The signal processing unit 21 performs a process of outputting a pulse signal for rotating the hollow motor 14 to the motor driver 23. The motor driver 23 supplies a drive current corresponding to the pulse wave signal from the signal processing unit 21 to the hollow motor 14, and the hollow motor 14 rotates the light detecting element 15. The type of the pulse wave signal outputted by the signal processing unit 21, the direction of rotation of the hollow motor ^ is determined, and the angle of rotation is determined by the number of pulse wave signals. The hollow motor 14 causes the light detecting element 15 to rotate the waveless signal corresponding angle in a direction corresponding to the signal from the signal processing unit 21, and then stops. Further, the signal processing unit 21 performs a process of measuring the commutation degree of the hollow motor unit that rotates the light detecting element 15 from the orthogonal polarization position from the rotational position based on the number of pulse signals that are output. The rotation angle of the hollow motor 14 can be measured by multiplying the angle at which the rotor is rotated by one step until the current rotational position of the rotation 1 to output the number of pulse signals for moving the hollow motor 14 to 1 =. 201118364 Fig. 3 is a conceptual diagram showing the relationship between the transmission axis of the light detecting element 15 that does not rotate and the polarizing surface of the linearly polarized light. The angle of rotation of the light detecting element 15 that is rotated from the initial rotational position by the hollow motor 14 is set to p. In the figure, the transmission axis of the rotating light detecting element 15 is shown at an angle of 9 〇. The angle formed by the direction and the transmission axis of the light detecting element 15 after the rotation is the rotation angle p. Further, in the figure, the direction orthogonal to the transmission axis of the light detecting element 15 is indicated by a broken line. The oscillating vibration is performed by the polarized surface of the linearly polarized light, and the polarized surface of the linearly polarized light incident on the light detecting element 15 and the transmission axis of the light detecting element 15 are vibrated at a frequency f by a φ (five) angle. The vibration angular width δ at this time is a vibration angular width corresponding to the amplitude of the vibration magnetic field generated by the Faraday coil 13. Among the linearly polarized lights incident on the rotating light detecting element 15, only the linearly polarized light component parallel to the transmission axis of the light detecting element 15 passes through the light detecting element 15. Light passing through the light detecting element 15 is received by the light receiving device 16. The light-receiving device 16 that has received the light outputs a light-receiving signal indicating the amount of received light by the voltage. The amplifying unit 24 amplifies the light-receiving signal and inputs it to the signal processing unit 21. As shown in FIG. 3, the (40) angle of the linearly polarized light incident surface incident on the light detecting element 15 and the transmission axis of the light detecting element 15 is vibrated by the frequency f. Therefore, the light detecting element ls included in the linear polarized light The size of the linearly polarized component parallel to the transmission axis also vibrates at the frequency f. Since the magnitude of the linearly polarized light component transmitted through the light detecting element 15 vibrates at the frequency f, the amount of light received by the light receiving device 16 that has received the light transmitted through the light detecting element 15 is vibrated at the frequency f. _ ' The light signal indicating the received light 1 in the light receiving device 16 by voltage is an alternating current signal that vibrates the voltage at the frequency f. The polarization plane of the linearly polarized light intersects the transmission axis of the light detecting element 15 2011 18364 2 The smaller the linear polarization component of the through-light detecting element 15 of the straight mid-angle, the vibration center of the translucent light (4) of the piece 15 is shaking again. The range of the angle between the polarizing surface and the transmission axis is the closest to the right angle. Therefore, the received light (4), "r, and the angle of the angle are dared to be processed by the processing unit 21. The signal of the signal of the feed material is shifted. The AC component extracted from the ^F Τf to the Faraday coil 13 is extracted, and the feedback control of the hollow motor is made so that the extracted AC is small. The signal processing unit 21 is controlled by feedback. To specify the τf technology component to achieve the smallest optical element
Iu無本★p ’並對所規定的轉動角度β進行測量。 =又—統销頻率f而振動的交流成分達到最小的狀態 下?H此轉動角度p的測量值為溶液試料的旋光度 精U上地理’旋光计對溶液試料的旋光度^進 定。 圖4疋表示本發明的旋光計的外觀的示意圖。旋光計 具備框體5 ’於框體5 a，設有圖i所示的内部結構。於 樞體5上形成有開口部’於開口部上設有可開閉的蓋51。 藉由將蓋51開放’可對框體5⑽設的樣本盒12注入溶 液試料。而且，於框體5的上表面，具備顯示部4。顯示 部4是與操作部25成為-體，且由使用液晶面板—a) 或電致發光（ele—neseenee ’ EL )面板等的觸控面板 (touch panel)所構成。再者，亦可為下述形態，即，由 顯示器（display)來構成顯示部4,由鍵盤（ke^b〇ard)等 201118364 2構絲作部25，藉此，使顯示部4與操作部2各別地構 度的顯示查ΐτ °M是包含用於顯示檢光元件15的轉動角 “動角、i的;構f面：5上表示用於顯示檢光元件15 的轉動來表-不畫的示意圖。顯示部4是包含以圖形 角度即檢光^號處理部21所測量时空馬達14的轉動 值來表的轉動角度的角度顯示部4卜及以數 成。^此以"夕ί疋件15的轉動角度的數值顯示部42而構 =。除此以外，顯示部4包含操作部25用的顯示畫面而構 的周圍疋包含圓圖形413、表示沿著圓圖形413 為;心而轉動度刻度412、及以將圓圖形413作 進行下述處理，即，每當測量中空馬達14的 對顯示部4所顯示的角度 ==，圖_在角度刻度412上指向所 的檢光元件15的轉動，箭=與/空馬達14的轉動相應 點為轉動中心而轉動，二二圓圖形413的中心 如此，角度顯示部41作為件15的轉動角度P。 件b的轉動角度的類比頭來表示逐次變化的檢光元 而且，信號處理部21進_〇§)旋轉計而發揮功能。 空馬達14的轉動角度時：_下述處理’即，每當測量中 42的顯示内容進行更新，不部4所顯示的數值顯示部 以使數值顯示部42顯示所測量 17 201118364 的轉動角度的值。因此，對應於盥中* 的檢光元件15的轉動，數值顯的轉動相應 的轉動角度β的值。如此，數值顯示 ^先疋件15 化的檢光元件15的轉動肖度的值 :、顯7^逐次變 ::::r旋光計具備對内部:二=1 ::計 =感測器所測定出的溫度顯示於 一而且，信號處理部21進行下述處理，即，對 文光部16的受光信號的強度，使顯 = 内的明暗發生變化。編言，錢處理部 制，即，使圓_413内的明暗發生變化，以使得以= 士 f而振動的交流成分的強度越大’圓圖形： =付越党，以受光信號的頻率f而振動的交流成分的強 度越小，圓圖形化_得越暗。顯示部4利用灰度 scale)來表現圓圖形413内的明暗，並根據信號處理 的控制而使明暗發生變化。 如上所述，本發明的旋光計中，顯示部4上顯示的角 度顯示部41作為類比旋轉計而發揮功能，於旋光度的測定 中，利用箭頭圖形411來指示逐次變化的檢光元件15的轉 動角度。使用者根據角度顯示部41上的箭頭圖形々η的變 化，可直觀地瞭解檢光元件15當前的轉動角度。而且，由 於箭頭圖形411最終指示的轉動角度為溶液試料的旋光 度，因此，使用者藉由觀察轉動角度的變化，可在旋光度 201118364 的測定結束之前，直觀地推測出旋光度是多少左右的值。 而且’本發明的旋光計中，數值顯示部42作為數位旋轉計 而發揮功能，以數值來表示檢光元件15的轉動角度。使用 者藉由確認數值顯示部42的顯示’可準確地獲知旋光度測 定中的檢光元件15的轉動角度以及溶液試料的旋光度。 繼而，對本發明的旋光計所進行的比旋光度測量以及 濃度測量的處理進行說明。旋光計對溶液試料的旋光度進 鲁 行計算，可使用液體試料的溶質的濃度來進行溶質的比旋 光度的測量、以及使用溶質的比旋光度來進行溶質的濃度 的測量。 ' 圖6是表示本發明的旋光計所進行的比旋光度測量的 處理流程的流程圖。在樣本盒12内未注入溶液試料的狀態 下’使用者對操作部25進行規定的操作，藉此，操作部 25接受初始設定開始的指示（S101)。根據初始設定開始 的指示，信號處理部21進行初始設定的處理，即，對中空 馬達14進行反饋控制，以使受光信號的強度達到最小，藉 • 此’決定處於正交偏光狀態的檢光元件15的初始轉動位 置’並將檢光元件15的轉動角度初始化為轉動角度〇。 (S102)。步驟S102結束後，信號處理部21亦可進行在 顯示部4上顯示催促溶液試料注入的訊息（message)的處 理。 繼而’旋光計藉由使用者打開蓋51後向樣本盒12内 注入溶液試料，從而接受溶液試料（S103)。繼而，信號 處理部21將用於輸入濃度的值的輸入晝面顯示於顯示部4 19 201118364 ^，稭由使用者操作—操作部25來輸 收所;受的溶液試料的溶質的漠度的值(二= 信號處理部21藉由佶用去剩w ) ，M而’ 從而歸者對㈣。卩25進行規定的操作， 攸而接收比％光度的測量開始的指示（幻〇5 )。 根據測量開始的指示，信號處理部2 的旋光度進行測定的處理⑶⑹。步驟s刚中= 理部21進彳了巾空馬達14的反馳制，藉此來使中空^ 14上的檢光元件15轉動，以使受光信號的強度變得更小，Iu has no version ★p ' and measures the specified angle of rotation β. = again - the frequency f of the system is pinned and the AC component of the vibration is minimized. H The measured value of the angle of rotation p is the optical rotation of the solution sample. The optical rotation of the solution is determined by the geography of the polarimeter. Fig. 4A is a schematic view showing the appearance of the polarimeter of the present invention. The polarimeter has a frame 5' on the frame 5a, and has an internal structure as shown in Fig. i. An opening portion is formed in the body 5, and an openable and closable cover 51 is provided on the opening. The sample sample 12 provided in the frame 5 (10) is filled with a solution sample by opening the lid 51. Further, the display unit 4 is provided on the upper surface of the casing 5. The display unit 4 is formed of a touch panel using a liquid crystal panel (a) or an electroluminescence (ele-neseenee ' EL) panel or the like. Further, the display unit 4 may be configured by a display, and the display unit 4 may be configured by a keyboard 18, such as a keyboard (key), thereby causing the display unit 4 to operate. The display of the respective components 2 is shown to include the rotation angle "moving angle, i" for displaying the photodetecting element 15; the f-plane: 5 indicates the rotation for displaying the photodetecting element 15 - The schematic diagram of the display unit 4 is an angle display unit 4 including a rotation angle of the time-space motor 14 measured by the inspection angle measuring unit 21, which is a pattern angle. In addition, the display unit 4 includes a display screen for the operation unit 25, and includes a circle pattern 413 and a circle pattern 413. The heart is rotated by the scale 412, and the circular pattern 413 is subjected to a process of measuring the angle displayed by the hollow motor 14 on the display portion 4 ==, and the image is pointed on the angle scale 412. The rotation of the light detecting element 15 is rotated by the arrow corresponding to the rotation of the air motor 14 as a center of rotation. The center of the shape 413 is such that the angle display portion 41 serves as the rotation angle P of the member 15. The analog head of the rotation angle of the member b indicates the successively detecting light detecting elements, and the signal processing portion 21 functions as a rotary meter. When the angle of rotation of the air motor 14 is _the following processing', that is, every time the display content of the measurement 42 is updated, the numerical value display portion displayed in the fourth portion is such that the numerical value display portion 42 displays the rotation angle of the measured 17 201118364 Therefore, the value corresponding to the rotation of the light detecting element 15 in the middle of the circle is numerically rotated by the value of the corresponding rotation angle β. Thus, the numerical value shows the degree of rotation of the light detecting element 15 of the first member 15 Value:, display 7^ successively change::::r The polarimeter has the internal temperature: two = 1: The temperature measured by the sensor is displayed in one, and the signal processing unit 21 performs the following processing, that is, The intensity of the light-receiving signal of the light-receiving unit 16 changes the brightness and darkness in the display. In other words, the money processing unit system changes the brightness in the circle _413 so that the communication is vibrated by =f The greater the intensity of the ingredients, the round figure: = paying the party, to accept The frequency f of the signal is smaller, and the intensity of the alternating component of the vibration is smaller, and the circular pattern is darker. The display unit 4 expresses the brightness and darkness in the circular pattern 413 by using the gradation scale, and changes the brightness and darkness according to the control of the signal processing. As described above, in the polarimeter according to the present invention, the angle display unit 41 displayed on the display unit 4 functions as an analog rotometer, and in the measurement of the optical rotation, the light detecting element 15 that sequentially changes is indicated by the arrow pattern 411. The angle of rotation of the light detecting element 15 can be visually understood by the user according to the change of the arrow pattern 々η on the angle display portion 41. Moreover, since the rotation angle finally indicated by the arrow pattern 411 is the optical rotation of the solution sample, the user can intuitively estimate the degree of the optical rotation before the end of the measurement of the optical rotation 201118364 by observing the change of the rotation angle. value. Further, in the polarimeter according to the present invention, the numerical value display unit 42 functions as a digital rotometer, and numerically indicates the rotation angle of the light detecting element 15. The user can accurately know the rotation angle of the light detecting element 15 in the optical rotation measurement and the optical rotation of the solution sample by confirming the display ' of the numerical value display portion 42'. Next, the specific optical rotation measurement and the concentration measurement processing performed by the polarimeter of the present invention will be described. The polarimeter calculates the optical rotation of the solution sample, and the concentration of the solute of the liquid sample is used to measure the specific rotation of the solute, and the specific optical rotation of the solute is used to measure the concentration of the solute. Fig. 6 is a flow chart showing the flow of processing of the specific optical rotation measurement performed by the polarimeter of the present invention. In a state where the sample sample is not injected into the sample cartridge 12, the user performs a predetermined operation on the operation unit 25, whereby the operation unit 25 receives an instruction to start the initial setting (S101). According to the instruction to start the initial setting, the signal processing unit 21 performs an initial setting process of performing feedback control on the hollow motor 14 to minimize the intensity of the received light signal, thereby determining the light detecting element in the orthogonal polarization state. The initial rotational position '15' initializes the rotational angle of the light detecting element 15 to the rotational angle 〇. (S102). After the end of step S102, the signal processing unit 21 may perform a process of displaying a message for prompting the injection of the solution sample on the display unit 4. Then, the sample is injected into the sample cartridge 12 by the user to open the lid 51, and the solution sample is received (S103). Then, the signal processing unit 21 displays the input surface for inputting the value of the density on the display unit 4 19 2011 18364 ^, and the straw is operated by the user-operating unit 25; the solute of the solution sample is indifference The value (two = the signal processing unit 21 uses w to remove w), M and 'there is the pair (4). The 卩25 performs the prescribed operation, and receives an instruction to start the measurement of the % luminosity (phantom 5). The processing (3) (6) of measuring the optical rotation of the signal processing unit 2 is performed in accordance with an instruction to start measurement. Step s just in the middle = the control unit 21 advances the reverse motor of the towel motor 14, thereby rotating the light detecting element 15 on the hollow tube 14 to make the intensity of the received light signal smaller.
於受光信號達到最小的階段，使檢光元件15的轉動停止。 信號處理部21獲取檢光元件15的最終的轉動角度的值， 以作為所測定出的旋光度的值。At the stage where the received light signal reaches a minimum, the rotation of the light detecting element 15 is stopped. The signal processing unit 21 acquires the value of the final rotation angle of the light detecting element 15 as the value of the measured optical rotation.
繼而’彳§號處理部21進行將所測定出的旋光度轉換成 特定的基準波長下的旋光度的處理（S107)。旋光度根據 用於測疋的直線偏光的波長而變化，基準波長的直線偏光 下的旋光度是使用基準波長的直線偏光來進行測定時應得 的旋光度。若將旋光計中用於旋光度測定的直線偏光的波 長設為λΐ，旋光計所測定出的旋光度設為α(λ1)，基準波 長設為λ0，基準波長下的旋光度設為α (λ0)，則旋光度的 波長依存性可用下述（1)式來表達。 α (λ0) = { 1.0 +Αχ (λ1-λ0)} χα (λΐ) ... (1) Ο)式中的Α為常數，使用藉由實驗而求出的值。（1) 式是對藉由實驗而獲得的直線偏光的波長與旋光度的關係 20 201118364 進行近似的近似式。（1)式中的係數{ι.〇 + Αχ (λ1_λ〇)} 表示使旋光計中使用的直線偏光的波長λι變為基準波長 λ0時，溶液試料的旋光度α (λΐ)所變化的比例。作為λ〇 的值’例如使用納的D線的波長的值，此時，= 3 nm。λΐ的值是藉由旋光計所具備的光源μ的發光波長以 及通過干涉濾光器32的光的波長來確定，是在製造旋光計 時實測所得。A、λ0以及λΐ的值由記憶部26預先吃憒。 步驟S107中，信號處理部21進行根據〇)式&計 * 算基準波長下的旋光度α (λ0)的處理。即，信號處理部 21讀出記憶部26中記憶的A、λ0以及λΐ，將a的|值乘以 自λΐ減去λ0所得的值後加上1.〇所得的值乘以所測定出 的旋光度α(λΐ)’藉此來計算基準波長下的旋光度α(λ〇)。 再者，信號處理部21亦可預先計算係數丨丨〇 + Αχ (λ1-λ0)}的值並記憶於記憶部26中，並 係數的值乘以旋光度α㈤的計算。而且，基^波^ 的值並不限於589.3 nm，旋光計亦可為使用其他的λ〇的 • 值來計算基準波長下的旋光度α (λ0)的形態。而且，旋 光计亦可為將多種λ〇的值記憶於記憶部26中，並計算多 ^基準波長下的旋光度α (λ〇)的形態。而且，旋光計亦 可為藉由使用者的操作來輸入λο的值’並使用所輪入的λ〇 的值來計算基準波長下的旋光度α (λ0)的形態。進而， 步，si^7中所用的式並不限於⑴s，旋光計亦可為根 據藉由貫驗或理論所獲得的、表示旋光度的波長依存性的 ()式以外的關係式或對照表（look-up table )等來計算 21 201118364 基準波長下的旋光度α (λ0)的形態。 步驟S107結束後’信號處理部21進行在顯示部4上 顯示所測定出的旋光度的值的處理（S108)。繼而，信號 處理部21進行下述處理，即，根據基準波長下的旋光度與 步驟S104中所接收的溶液試料的溶質的濃度的值，來計 算溶質的比旋光度（S109)。若將比旋光度設為[α]，旋光 度測定時直線偏光透過溶液試料的長度設為L，溶質的濃 度設為C，則比旋光度[α]可用下述（2)式來表示。 [α] = α (λ0) / (LxC) ... (2) (2)式是使用基準波長λ0的直線偏光來進行測定時 所獲得的比旋光度的定義式。L的值是根據旋光計所具備 的樣本盒12的大小來確定的值，並由記憶部26預先記憶。 步驟S109中’信號處理部21進行根據（2)式來計算比 方疋光度[α]的處理。即，信號處理部21讀出記憶部26中記 憶的L，並將基準波長下的旋光度α (χ〇)除以將所接收 的C乘以所讀出的L所得的值，藉此來計算比旋光度!^]。 再者，旋光計亦可為下述形態，即，可使用多種大小的樣 本盒12，藉由使用者的操作來接收與所用樣本盒12的大 小相應的L的值，並根據所接收的l的值來計算比旋光度 [α]。 步驟S109結束後，信號處理部21在顯示部4上顯示 所叶异出的比旋光度[α]的值（S110)，並結束比旋光度測 22 201118364 量的處理。再者，旋光計亦可為連接於未圖示的個人 嶋1⑺叫咖，PC)或印表機（printer)等，並:用 PC或印表機等來輸出所測量的喊光度的形態。 、圖7是表示本發明的旋光計所進行的濃度測量的處 流程的流程圖。在樣本盒12内未注人溶液試料的狀態下， 操作部25接受初始設定開始的指示（S201)，信號^理呷 21進行初始設定的處理（S2〇2)。、繼而，旋光計接 ^ 試料（S203 )。信號處理部21將用於輸入溶液試料的溶 的比旋光度的值的輸入晝面顯示於顯示部4上，藉由使用 者操作-操作部25來輸人比旋光度的值，||此來接收所接 受的溶液試料的溶質的比旋光度的值（S2〇4)。繼而，作 號處理部21藉由使用者對操作部25進行規定的操作，藉 此來接收濃度測量開始的指示（S2〇5)。根據測量開始^ 指不’彳§號處理部21進行對溶液試料的旋光度進行測定的 處理（S206)。繼而，信號處理部21進行將所測定出的旋 光度轉換成基準波長下的旋光度的處理（S2〇7)。繼而， • 信號處理部21將轉換後的旋光度的值顯示於顯示部4上 (5208) 〇 步驟S208結束後，信號處理部21進行下述處理，即， 根據轉換後的基準波長下的旋光度與步驟S2〇4中所接收 的溶液試料的溶質的比旋光度的值，來計算溶質的濃度 (5209) 。藉由對前述的（2)式進行變形，溶質的濃度c 可用下述的（3)式來表示。 又 23 201118364 C = a (λ0) / (Lx[a]) ... (3) 步驟S209中，信號處理部21進行根據（3)式來計 异溶液試料的溶質的濃度的處理。即’信號處理部讀出 記憶部26中記憶的L，並將基準波長下的旋光度α (λ〇) 除以將所接收的比旋光度[α]乘以所讀出的L所得的值，藉 此來計算溶質的濃度C。步驟S209結束後’信號處理部 21在顯示部4上顯示所計算出的濃度C的值（S21〇)，並 結束濃度測量的處理。再者，旋光計亦可為使用未圖示的 PC或印表機等來輸出所測量的濃度的形態。 如以上所詳述，本發明的旋光計對溶液試料的旋光度 進行測定，並將所測定出的旋光度轉換成使用基準波長λ〇 的直線偏光來進行測定時應得的基準波長下的旋光度α (λ0 )。當溶液試料的溶質的濃度為已知時，旋光計根據基 準波長下的故光度與溶液試料的溶質的濃度，來計算溶質 的比旋光度。由於用於旋光度測定的光的波長存在偏差， 因此所測定的旋光度的值會根據旋光計而有所偏差，但藉 由將旋光度轉換成基準波長下的旋光度，旋光度的值的偏 差得以修正。由於本發明中求出的比旋光度是根據基準波 長下的旋光度而計异，因此不會產生因旋光計的不同造成 的比旋光度的偏差，從而可實現與由其他旋光計所測量的 比旋光度的單純比較。尤其，當設基準波長λ〇=589 3 nm 時，可對文獻中記載的值等利用對光源使用鈉燈的旋光計 而在過去測定的比旋光度的值、與利用本發明的旋光計所 24 201118364 測量的比旋光度的值進行單純比較。 而且，本發明的旋光計同樣地，當溶液試料的溶質的 比旋光度為已知時，根據轉換後的基準波長下的旋光度與 溶液試料的溶質的比旋光度來計算溶液試料中的溶質的^ 度。由=本發明中所獲得的濃度是根據基準波長下的旋光 度而計算，因此不會產生⑽料的不同造成的偏差，從 而可對由其他旋光計所測量的濃度的值以及過去求出的濃 度的值、與利用本發明的旋光計所測量的濃度的值進行單 參 純比較。而且，藉由將光源31設為LED，可實現旋光計 的小型化以及低成本化。 雖然本發明已以較佳實施例揭露如上，然其並非用以 限，本發明，任何熟習此技藝者，在不脫離本發明之精神 和範圍内，當可作些許之更動與潤飾，因此本發明之保護 範圍當視後附之申請專利範圍所界定者為準。 【圖式簡單說明】 圖1是表示本發明的旋光計的内部結構的結構圖。 馨圖2A是表示直線偏光的偏光面的變化的概念圖。 圖2B是表示直線偏光的偏光面的變化的概念圖。 圖2C是表示直線偏光的偏光面的變化的概念圖。 圖3是表示轉動的檢光元件的透過軸與直線偏光的偏 光面的關係的概念圖。 圖4是表示本發明的旋光計的外觀的示意圖。 圖5是表示用於顯示檢光元件的轉動角度的顯示畫面 的示意圖。 25 201118364 圖6是表示本發明的旋光計所進行的比旋光度測量的 處理流程的流程圖（flow chart)。 圖7是表示本發明的旋光計所進行的濃度測量的處理 流程的流程圖。 【主要元件符號說明】 4 :顯示部 5 :框體 11 :偏光元件 12 :樣本盒 13 :法拉第線圈 14 :中空馬達 15 :檢光元件 16 :受光器件 21 :信號處理部 22 :振盪器 23 :馬達驅動器 24 :放大部 25 :操作部 26 :記憶部 30 :點燈電路 31 :光源 32 :干涉濾光器 33、34 :透鏡 41 :角度顯示部 26 201118364 42 :數值顯示部 51 :蓋 411 :箭頭圖形 412 :角度刻度 413 :圓圖形 (X .比先度 β:轉動角度 δ:振動角寬度Then, the processing unit 21 performs a process of converting the measured optical rotation into an optical rotation at a specific reference wavelength (S107). The optical rotation varies depending on the wavelength of the linearly polarized light used for the measurement of the enthalpy, and the optical rotation under the linear polarization of the reference wavelength is the optical rotation obtained when the linear polarization of the reference wavelength is used for measurement. When the wavelength of the linearly polarized light used for the optical rotation measurement in the polarimeter is λΐ, the optical rotation measured by the polarimeter is α (λ1), the reference wavelength is λ0, and the optical rotation at the reference wavelength is α ( Λ0), the wavelength dependence of the optical rotation can be expressed by the following formula (1). α (λ0) = { 1.0 + Αχ (λ1 - λ0)} χ α (λΐ) (1) The Α in the formula Α is a constant, and the value obtained by experiment is used. (1) The equation is the relationship between the wavelength of the linearly polarized light obtained by the experiment and the optical rotation. 20 201118364 An approximate approximation is performed. The coefficient {ι.〇+ Αχ (λ1_λ〇)} in the formula (1) indicates the ratio of the optical rotation α (λΐ) of the solution sample when the wavelength λι of the linearly polarized light used in the polarimeter is changed to the reference wavelength λ0. . As the value of λ ’ ' for example, the value of the wavelength of the D line of the nano is used, and at this time, = 3 nm. The value of λ 确定 is determined by the wavelength of the light source μ of the polarimeter and the wavelength of the light passing through the interference filter 32, which is actually measured when the polarimeter is manufactured. The values of A, λ0, and λΐ are preliminarily eaten by the memory unit 26. In step S107, the signal processing unit 21 performs a process of calculating the optical rotation degree α (λ0) at the reference wavelength based on the equation & In other words, the signal processing unit 21 reads A, λ0, and λΐ stored in the memory unit 26, multiplies the value of a by the value obtained by subtracting λ0 from λΐ, and adds the value obtained by 1.〇 to the measured value. The optical rotation α (λΐ)' is used to calculate the optical rotation α (λ〇) at the reference wavelength. Further, the signal processing unit 21 may calculate the value of the coefficient 丨丨〇 + Αχ (λ1 - λ0)} in advance and store it in the memory unit 26, and multiply the value of the coefficient by the calculation of the optical rotation degree α (f). Further, the value of the base wave is not limited to 589.3 nm, and the polarimeter may calculate the optical rotation degree α (λ0) at the reference wavelength using other values of λ • . Further, the polarimeter may store a plurality of values of λ〇 in the memory unit 26, and calculate the form of the optical rotation α (λ〇) at a plurality of reference wavelengths. Further, the polarimeter may also input the value of λο by the user's operation and calculate the optical rotation α (λ0) at the reference wavelength using the value of λ 轮 that is rotated. Further, in the step, the formula used in si^7 is not limited to (1) s, and the polarimeter may be a relational expression or a comparison table based on the () formula indicating the wavelength dependence of the optical rotation obtained by the experiment or the theory. (look-up table) or the like to calculate the form of the optical rotation α (λ0) at the reference wavelength of 201118364. After the end of step S107, the signal processing unit 21 performs a process of displaying the value of the measured optical rotation on the display unit 4 (S108). Then, the signal processing unit 21 performs a process of calculating the specific optical rotation of the solute based on the optical rotation at the reference wavelength and the value of the concentration of the solute of the solution sample received in the step S104 (S109). When the specific optical rotation is set to [α], the length of the linearly polarized light-transmitting solution sample in the measurement of the optical rotation is L, and the concentration of the solute is C, the specific optical rotation [α] can be expressed by the following formula (2). [α] = α (λ0) / (LxC) (2) The equation (2) is a definition formula of the specific optical rotation obtained when the linear polarized light of the reference wavelength λ0 is used for measurement. The value of L is a value determined based on the size of the sample cartridge 12 provided in the polarimeter, and is memorized in advance by the memory unit 26. In step S109, the signal processing unit 21 performs a process of calculating the ratio luminosity [α] according to the equation (2). In other words, the signal processing unit 21 reads the L stored in the storage unit 26 and divides the optical rotation α (χ〇) at the reference wavelength by the value obtained by multiplying the received C by the read L. Calculate the specific optical rotation! ^]. Furthermore, the polarimeter may be in the form of a sample cartridge 12 of various sizes, which is operated by a user to receive a value of L corresponding to the size of the sample cartridge 12 used, and according to the received The value is calculated to calculate the specific optical rotation [α]. After the end of step S109, the signal processing unit 21 displays the value of the specific optical rotation [α] of the leaf out on the display unit 4 (S110), and ends the processing of the optical rotation measurement 22 201118364. Further, the polarimeter may be connected to a personal 嶋1 (7) not shown (PC), a printer, or the like, and the form of the measured smear is outputted by a PC or a printer. Fig. 7 is a flow chart showing the flow of concentration measurement by the polarimeter of the present invention. In a state where the sample sample is not filled in the sample cartridge 12, the operation unit 25 receives an instruction to start the initial setting (S201), and the signal processing unit 21 performs the initial setting processing (S2〇2). Then, the polarimeter is connected to the sample (S203). The signal processing unit 21 displays an input surface for inputting the value of the dissolved specific optical rotation of the solution sample on the display unit 4, and the user operates the operation unit 25 to input the value of the specific optical rotation, || The value of the specific optical rotation of the solute of the received solution sample is received (S2〇4). Then, the number processing unit 21 receives a predetermined operation from the operation unit 25, thereby receiving an instruction to start concentration measurement (S2〇5). The processing for measuring the optical rotation of the solution sample is carried out based on the measurement start (refer to the processing unit 21) (S206). Then, the signal processing unit 21 performs a process of converting the measured optical rotation into the optical rotation at the reference wavelength (S2〇7). Then, the signal processing unit 21 displays the value of the converted optical rotation on the display unit 4 (5208). After the step S208 is completed, the signal processing unit 21 performs a process of rotating the light according to the converted reference wavelength. The concentration of the solute is calculated by the value of the specific optical rotation of the solute of the solution sample received in step S2〇4 (5209). By modifying the above formula (2), the concentration c of the solute can be expressed by the following formula (3). Further, in the step S209, the signal processing unit 21 performs a process of calculating the concentration of the solute of the solution sample according to the formula (3). That is, the 'signal processing unit reads L stored in the memory unit 26, and divides the optical rotation α (λ〇) at the reference wavelength by the value obtained by multiplying the received specific optical rotation [α] by the read L. In order to calculate the concentration C of the solute. After the end of step S209, the signal processing unit 21 displays the value of the calculated density C on the display unit 4 (S21〇), and ends the process of concentration measurement. Further, the polarimeter may be in the form of outputting the measured concentration using a PC or a printer (not shown). As described in detail above, the polarimeter of the present invention measures the optical rotation of the solution sample, and converts the measured optical rotation into linear light of the reference wavelength λ 来 to determine the optical rotation at the reference wavelength. Degree α (λ0 ). When the concentration of the solute of the solution sample is known, the polarimeter calculates the specific optical rotation of the solute based on the luminosity at the reference wavelength and the concentration of the solute of the solution sample. Since the wavelength of the light used for the measurement of the optical rotation is deviated, the value of the measured optical rotation varies depending on the polarimeter, but by converting the optical rotation into the optical rotation at the reference wavelength, the value of the optical rotation The deviation is corrected. Since the specific optical rotation obtained in the present invention is different depending on the optical rotation at the reference wavelength, variation in specific optical rotation due to the difference in the polarimeter does not occur, and measurement with other polarimeter can be realized. A simple comparison of specific rotations. In particular, when the reference wavelength λ 〇 = 589 3 nm, the value of the specific optical rotation measured in the past using the polarimeter using a sodium lamp for the light source, and the polarimeter using the present invention can be used for the values described in the literature. 201118364 The measured specific optical rotation values are simply compared. Further, in the polarimeter of the present invention, when the specific optical rotation of the solute of the solution sample is known, the solute in the solution sample is calculated based on the optical rotation at the reference wavelength after the conversion and the specific optical rotation of the solute of the solution sample. ^ degrees. The concentration obtained by the present invention is calculated based on the optical rotation at the reference wavelength, so that the deviation due to the difference in the material (10) is not generated, so that the value of the concentration measured by the other polarimeter and the value obtained in the past can be obtained. The value of the concentration was compared with the value of the concentration measured by the polarimeter of the present invention. Further, by using the light source 31 as an LED, it is possible to reduce the size and cost of the polarimeter. While the present invention has been described in its preferred embodiments, the present invention is not intended to be limited thereto, and it is to be understood that the invention may be modified and modified without departing from the spirit and scope of the invention. The scope of the invention is defined by the scope of the appended claims. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a configuration diagram showing the internal structure of a polarimeter according to the present invention. Xin 2A is a conceptual diagram showing a change in a polarizing surface of linearly polarized light. Fig. 2B is a conceptual diagram showing a change in a polarizing surface of linearly polarized light. 2C is a conceptual diagram showing a change in a polarizing surface of linearly polarized light. Fig. 3 is a conceptual view showing a relationship between a transmission axis of a rotating photodetecting element and a polarizing surface of linearly polarized light. Fig. 4 is a schematic view showing the appearance of a polarimeter according to the present invention. Fig. 5 is a schematic view showing a display screen for displaying the rotation angle of the light detecting element. 25 201118364 Fig. 6 is a flow chart showing a flow of processing of specific optical rotation measurement by the polarimeter of the present invention. Fig. 7 is a flow chart showing the flow of processing for concentration measurement by the polarimeter of the present invention. [Description of main component symbols] 4 : Display portion 5 : Frame 11 : Polarizing element 12 : Sample cartridge 13 : Faraday coil 14 : Hollow motor 15 : Light detecting element 16 : Light receiving device 21 : Signal processing portion 22 : Oscillator 23 : Motor driver 24: Amplifying portion 25: Operating portion 26: Memory portion 30: Lighting circuit 31: Light source 32: Interference filter 33, 34: Lens 41: Angle display portion 26 201118364 42: Numerical value display portion 51: Cover 411: Arrow graph 412: angle scale 413: circle pattern (X. ratio first degree β: rotation angle δ: vibration angle width