TW201018907A - Handheld biological specimen examination apparatus and method thereof - Google Patents
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Abstract
Description
201018907 六、發明說明： 【發明所屬之技術領域】 β本發StC關於#生物喊#檢測裝置及其相應方法，特別 是關於-種手持式生物試片檢測裳置及其相應方法。 【先前技術】 隨著生物馳輕展，生她速檢職#的研究已漸趨完 相車乂於傳統的逐-檢測方式，生物試片檢測檢測技術的發 展加快了檢測分析眺行，且有利於-次平行檢測多種分析物 與反應。 現常，用之生物試片主要有長條形試片與陣列式生物晶 片兩種m利用長條形生物試片（呂吨Test)進行檢測的常 見應用包括了驗孕試片與檢測病毒性試劑(如c型肝炎之沉乂 (Hepatitis C virus)、動物性病毒、植物性病毒等）；其試片外 . 觀如第一 A圖所示，長條形生物試片100具有一作用區110 以供滴入受測者的血液、尿液或體液等，在受測區域D中則 包含用於檢測時判斷試片是否為有效品之控制線12〇以及至 參 少一測試線130 ’其用於判讀相應之生化反應結果；以三明治 反應為例，在檢測時’如控制線12〇出現呈色則表示該試片為 一有效品，而測試線130的出現則代表陽性反應結果，反之為 陰性反應結果。 至於陣列式生物晶片則包括用於判讀待測樣品基因型態 之基因晶片或用於判讀待測樣品之抗原、抗體種類之蛋白質晶 片，其外觀分別如第一 B圖與第一 c圖所示；陣列式生物晶 片105係於一基底115上預先以各式反應試劑與呈色物質形成 有複數個測試圓點125a、125b而形成一圓點陣列，根據待測 樣品與各式反應試劑是否產生反應，在圓點陣列的不同圓點處 201018907 係產生壬色而形成圖樣。一般而言’目前常見以人眼判讀方式 來根據呈色圖樣判讀其對應之基因型態，其不但耗費時間與人 力，亦容易產生判讀差異而導致檢測結果有誤。 目前亦發展出多種利用儀器進行判讀之生物試片檢測裝 置’然其多藉由掃瞄方式對生物試片進行逐行逐列地掃瞄而將 待測生物試片之影像輸入於檢測裝置中，再將輸入之影像與内 建於檢測裝置巾喊大資料庫進行比對關讀檢測絲，不但 耗費時間，亦無法對檢測結果進行進一步之定量或半定量分201018907 VI. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a detection device and a corresponding method thereof, and more particularly to a handheld biological test strip detection device and a corresponding method thereof. [Prior Art] With the development of bio-sports, the research on the rapid test of her has gradually completed the traditional one-by-detection method, and the development of biological test strip detection technology has accelerated the detection and analysis. Conducive to - parallel detection of multiple analytes and reactions. At present, the biological test strips mainly include long strip test strips and array biochips. Common applications for the detection of long strip biological test strips (Lutt Test) include pregnancy test strips and detection of virality. Reagents (such as Hepatitis C virus, animal virus, plant virus, etc.); outside the test piece. As shown in Figure A, the strip-shaped biological test piece 100 has an action area. 110 for dripping into the blood, urine or body fluid of the subject, and in the area D to be tested, the control line 12 for determining whether the test piece is effective or not, and the test line 130' for the test piece are included in the test area D. It is used to interpret the corresponding biochemical reaction results; taking the sandwich reaction as an example, if the color is displayed on the control line 12〇, the test piece is a valid product, and the appearance of the test line 130 represents a positive reaction result. The opposite is the result of a negative reaction. The array type biochip includes a gene wafer for reading the genotype of the sample to be tested or a protein wafer for reading the antigen and antibody type of the sample to be tested, and the appearance thereof is as shown in the first B diagram and the first c diagram, respectively. The array biochip 105 is formed on a substrate 115 by forming a plurality of test dots 125a, 125b with various reactive reagents and coloring materials to form an array of dots, according to whether the sample to be tested and various reactive reagents are generated. The reaction, at the different dots of the dot array, produced a pattern in 201018907. Generally speaking, it is common to use human eye interpretation methods to interpret the corresponding genotype according to the color rendering pattern, which is not only time-consuming and human, but also easy to produce interpretation differences and lead to incorrect detection results. At present, a variety of biological test strip detection devices using instruments have been developed. However, the biological test strips are scanned line by line by scanning, and the images of the biological test strips to be tested are input into the detecting device. Then, the input image is compared with the detection device and the large database is used to check the detection silk, which not only takes time, but also can not further quantify or semi-quantify the detection result.
析’且為簡化比對勒呈及提高比對正確性，生物試片的檢測點 亦受限於圓點型態。 ‘ ，外，現有各式生物試片檢測裝置不但設備龐大，且其皆 型態的生物試片而加以設計，其試片載具或處理模組 等僅與单-鶴相容，無法同時針對長條形生物試片與陣列式 =晶片進行檢測分析；換言之，現有生物試片檢測裝置僅支 物試片或陣列式生物晶片之檢測姻必須使用兩 。义始可分別進行長條形生物試片或陣列式生物晶片 之檢測。 於神職「請人經悉心試驗與研究’並—本鎮而不捨之 月申W本案讀式生物制制裝 1置具有朗可攜，且與财手持裝t =裝置更可支财_態生物制之檢測，極具顧發展優 【發明内容】 本發明之第-構想在於提供—種 居家檢測之用 積輕巧，且與現有手持式通城iii 201018907 署構想在於提供—種傾式生物抑檢測裝 之旦/傻ίΐί測生物試片影像之榻取、配合搭載於檢測褒置内、 量=處_組的演算處理，可對生物試片檢測結果進行半定 ^發明之第二構想在於提供—種手持式生物試片檢測 置’其可檢測長條形與陣列式等各種不同型態之生物、 包括根據縣，所提供之傾式生物試片檢測裝置 ❶ 用於娜該制生物制之影像；—影像處= 、斜、輕接至像娜模組，以根據該制生物試片之型離 :對所擷取之影像進行影像解減理；—中央處理單元，其= f並控制該雜處理.；以及—輸出模組，其純至該巧傻 理模組以輸出與該相對反應強度相應之影像解析結果二。 像處ίϊί 想，本㈣之手赋生物試片_裝置中的影 處，模組包括前置處理單元與—影像解析單元。該 取之影像進行優化處理以提升影像品f;該影像 斤早福接至該前置處理單元錄據該制 態而對經優域理之歸進行下述處t ^ (a)對經優化處理之影像進行灰階化處理； ()基於灰1¾化處理結果進行影像定位，.以確定一分析 區域；及 (c)比較該分析區域之灰階值與一參考值，以得一相 反應強度； —根f上述構想’本發明之手持式生物試片檢測裝置更包括 —光源模組，其祕魏巾央處理單元並根雜制生物 之型態而提供與其對應之一色光。 _根據上述構想，該影像處理模組中更包括一影像放大單 兀，其耦接至該前置處理單元，用於放大所擷取之影像。 201018907 檢測裝置與-外部裝置通ζ央處理早减使該手持式生物試月 置包 影像處理模組，·以及-生物試片=置： 置輯行錄制檢測之半定量分析。 输纖 像像齡模組褐取該待測生物試片之影 像該以象處理模組根據該待測生物試片之型 影像進行縣優化處理與影像騎處理，以得$定量 結果而供該傾式通喊置顯轉輸出。⑤象解析 根據上述構想，其令該生物試片分析裝 放n ’用於放大該生物待測試片以供影像之擷取。〜 根據上述構想’本剌之顿式生物試#檢 該手持式通訊裝置而與一外部裝置通訊。’、g 位助==想，靖她裝職行_或個人數 称本發明同時提出了一種生物試片檢測方法其包括下列步 ⑻定向一待測生物試片； (b) 操取該待測生物試片之影像； (c) 對所擷取之影像進行優化處理·， 定量影像進行影像解析處理，以得一半 201018907 (e) 輪出該影像解析結果。 根據上述構想’在本發明之生物試片檢測方法於步驟⑻ 後’更包括步驟(al).根據該待測生物試片之型態，以一對應 顏色之光源照射該待測生物試片。 根據上述構想，在本發明之生物試片檢測方法的步驟⑹ 中，所述優化處理包括： (cl)消除所擷取之影像中之雜訊；及 (c2)對所擷取之影像進行影像去邊處理。 根，上述構想，在本發明之生物試片檢測方法的步驟(d) 中，所述影像解析處理包括： (dl)對所擷取之影像進行灰階化處理； (d2)疋位經灰階化處理之影像，以確定一分析區域；以 及 (d3)比較該分析區域之灰階值與一參考值’以得該半定 量影像解析結果。 本案得藉由下列圖式及詳細說明，俾得以令讀者更深入了 解：In order to simplify the alignment and improve the correctness of the alignment, the detection points of the biological test piece are also limited by the dot pattern. In addition, the existing various biological test strip detection devices are not only equipped with large devices, but also designed for biological test strips. The test strip carriers or processing modules are only compatible with single-heavy cranes, and cannot be simultaneously targeted. The long strip biological test piece and the array type = wafer are subjected to detection and analysis; in other words, the existing biological test piece detecting device only uses two test pieces for the test piece or the array type biochip. Yishou can perform long strip biological test strips or array biochips. In the priesthood, "please carefully test and study" and - the town's unrelenting month, the W-reading biological system is equipped with a portable device, and with the financial hand-held device t = device more financially _ state The detection of biological system is very good for development. [Inventive content] The first concept of the present invention is to provide a light-weight home-based test, and the existing hand-held Tongcheng iii 201018907 is conceived to provide a kind of tilting biological suppression. Detecting the packaging / silly ΐ ΐ 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测 测Providing a hand-held biological test strip detection device that can detect various types of organisms such as strips and arrays, including the tilting biological test strip detection device provided by the county, Image; - image area =, oblique, lightly connected to the like module, according to the type of the biological test strip: image decompression of the captured image; - central processing unit, which = f and Control the processing; and - the output module, It is pure to the clever module to output the image analysis result corresponding to the relative reaction intensity. Like the ϊ ϊ 想 , 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本 本And the image analysis unit. The image is optimized to enhance the image product f; the image is connected to the preprocessing unit to record the state of the image, and the following is performed on the image of the superior domain. (a) performing grayscale processing on the optimized image; () performing image localization based on the grayscale processing result to determine an analysis region; and (c) comparing the grayscale value of the analysis region with a reference value In order to obtain the intensity of one phase reaction; - the above concept of the root f. The hand-held biological test strip detecting device of the present invention further comprises a light source module, and the secret processing unit of the Weiwei towel is provided in parallel with the type of the living creature. According to the above concept, the image processing module further includes an image magnifying unit coupled to the pre-processing unit for enlarging the captured image. 201018907 detecting device and external device ζCentral processing early reduction Hand-held biological test monthly package image processing module, and - biological test piece = set: semi-quantitative analysis of recording detection of the line. The fiber-optic image age module browns the image of the biological test piece to be tested. The image processing module performs county optimization processing and image riding processing according to the type image of the biological test piece to be tested, so as to obtain a quantitative result for the tilting and screaming display output. 5 image analysis according to the above concept, which makes the The biological test piece analysis loading n' is used to enlarge the biological test piece for image capture. ~ According to the above concept, 'Benton's type of biological test # detects the handheld communication device and communicates with an external device.' , g position help == think, Jing her loading line _ or personal number said the invention also proposed a biological test piece detection method comprising the following steps (8) to direct a biological test piece to be tested; (b) to operate the test (c) Optimize the captured image, and quantize the image for image analysis to obtain half of 201018907 (e). According to the above concept, the biological test strip detecting method of the present invention further includes the step (al) after the step (8). According to the type of the biological test piece to be tested, the biological test piece to be tested is irradiated with a light source of a corresponding color. According to the above concept, in the step (6) of the biological test strip detecting method of the present invention, the optimization processing comprises: (cl) eliminating noise in the captured image; and (c2) performing image on the captured image. Go to the side. Root, the above concept, in the step (d) of the biological test strip detecting method of the present invention, the image parsing processing comprises: (dl) gray-scale processing of the captured image; (d2) 疋 position gray The processed image is determined to determine an analysis region; and (d3) the grayscale value of the analysis region is compared with a reference value to obtain the semi-quantitative image analysis result. The following diagrams and detailed explanations can be used to give readers a better understanding:
【實施方式】 、本發明將參閱含有本發明較佳實施例之所附圖示予 以充分描述’但在此描述之前應瞭解熟悉本行技藝之人士可修 改本文中所描述之發明，同時獲致本發明之功效。因此，需瞭 解以下之描述對熟悉本行技藝之人士而言為一廣泛之揭示且 其内容不在於限制本發明。 5奮參閱第二圖’其係本發明之一較佳實施例之模組方塊 圖’用於說明本翻之手持式生物則制裝置的模組配置。 如第二圖所示’本發明之手持式生物檢測裝置主要由手持 ;通訊裝f部分之模、组2〇與生物試片放大裝置之模組25整合 7 201018907 而成。在手持式通訊裝置部分之模組2() 2〇1，其控制整個生物試片檢測過 早打，手持式通訊褒置部分之模組2〇中另i有二 像擷取模組202、影像處理模組2G3、輪出模組2G4中 模組205，其分別祕至中央處理單元2〇ι以接收並 ^ 2〇1之控舰號而動作，其中，影 $ 〇 _態的生物試片進行影像^斤^ 本發月之生物试片分析裝置上設有一試片載且The present invention will be fully described with reference to the accompanying drawings in which the preferred embodiments of the present invention are described, but it is understood that those skilled in the art can modify the invention described herein while obtaining the present invention. The efficacy of the invention. Therefore, it is to be understood that the following description is not a limitation of the invention. 5 is a block diagram of a preferred embodiment of the present invention for explaining the module configuration of the hand-held biometric device of the present invention. As shown in the second figure, the hand-held biodetection device of the present invention is mainly composed of a hand-held device, a module of the communication device, a group 2, and a module 25 of the biological test chip amplifying device. In the module 2() 2〇1 of the handheld communication device, the whole biological test piece is detected to be detected prematurely, and the module 2 of the handheld communication device has another two image capturing module 202, The image processing module 2G3 and the module 205 of the wheel-out module 2G4 are respectively secreted to the central processing unit 2〇1 to receive and control the number of the control ship, wherein the biological test of the shadow 〇_ state The film is imaged ^^^ The test piece of the biological test piece of the month is provided with a test piece and
=不同型態的待測生物試片（例如：長條形試片、、陣列= 物晶片等）供，像操取模組2〇2進行影像榻取；模組^ = 置有影像放大單元252、光源模組253以及電源模组254，盆 中光源模組253係接收電源模組25續供 力而 ^ 型態的制生物試片提供獨色光之絲。冑力續對不同 在本發明-較佳實施例中，係使用手持式觀裝置（例 ^ ·仃動電話或個人數位助理）中的中央處理單元2q ^ =取模組202、影像處理模組2〇3、輸出模組2〇4來進行= 操取、分析無心並_其觀魏與外部裝置進行資料= 換與傳輸。絲減本發财_，亦可自行開發之 j組配合_之適當法，精影像娜、分析與顯 作0 ί二 本㈣之手持式生物刹檢測裝 置之外觀Μ®。本發明之手持式生物試片檢職们〇〇主要 係由手持式通訊裝置31〇以及可與其搭載之暗房裝置32〇所構 成’該手持式通訊裝f 310與該暗房裝置32〇时別建置有如 第二圖所示之模組20與25。 配合參閱第三B圖’其咖了本發明之手持式生物試片 檢測裝置300的主要結構組成。根據本發明之較佳實施例，， 手持式生物試片檢測裝|300中的手持式通訊裝£31〇係支援 201018907 ft能之任何—種手持式通訊裝置，包括行動魏、個人數 助理（PDA)與任何該領域技術人士習知的通訊裳置豆 3 315以呈現檢測聲除前述通訊敍外:Ϊ 了使用/、他可執行本發明之影像處理運算方法之手 Ζ可不具通訊功能）’以擷取並解析待測生物試片之影、= 輸出對應的檢測結果， / 暗房裝置320係建構於由上、下外殼32卜322所構 土座内，其内含-影像放大裝置（例如單 鲁 ，模組324以及一電源模組奶=== 组325 ^連接，其接收電力後根據待測生 有一試片ST而提供不同色光之光源進行補光。基座上具 有试片载具326，作為容置待測生物試片之用。 請參閱第四圖’其係第三Β圖中所示之試片載具幻 大圖。本發明之手持式生物試#檢測裝置的試片載且 干用於承載不同型態的生物試片。如第= 斤 ^片载八326上具有複合凹槽之設計，所述 开 所構成，其中長條 ==Τίί如第—Α圖麻織形生物試片， 望驗、病毒性試劑（如檢測C浙炎之HCV試劑） 例如丨^槽328係用於容置—陣列式生物晶片400 ’ 未樣品之生物學特徵的微陣列晶片用於判讀 未°樣"°之抗原、抗體種類的蛋白質晶片等。 所噴知樣品之生物學特徵檢測時’微陣列晶.片上 _\=—序具有一定的反應意義，故於進行檢測與判讀 “必須ϋ定生物晶片上檢測點陣 i 檢測分析，在本發明之手持式生物ί片= 某—角落形成定向:十，即於陣列式試片槽328之 械斜緣328A，其與生物晶片400上固定方向之 201018907 斜緣400A相符；藉此，檢測者在置 上時’即可對試片進行快速定向 放生物試片於試片載具 326 及其3^第五聽明本發明之傾式生物試片檢測裝置 由一較佳實施例中’係利用現有手持式通訊裝置 中无，之1、相裝置進仃制生物晶片之影像擷取，並於其中央 ，理單70與既備之影像處理模組巾建立影像處理運算法 行試片影像之解析。 …在進行生物試片檢測時，首先將手持式通訊裝置以及與盆 ^載之生物試片放大裝置（即暗房裝置）組裝在一起而形成^ ，之手持式生物制檢職置’並依待啦物試片之型態而 將其疋向於試片載具上。= different types of biological test specimens to be tested (for example: long strip test strips, array = wafers, etc.), such as the operation module 2〇2 for image couching; module ^ = image magnifying unit 252, the light source module 253 and the power module 254, the light source module 253 in the basin receives the continuous power supply of the power module 25, and the biological test piece of the type provides the filament of the monochromatic light. In the present invention - the preferred embodiment, the central processing unit 2q ^ = module 202, image processing module is used in a handheld device (eg, a mobile phone or a personal digital assistant) 2〇3, output module 2〇4 to carry out = operation, analysis is unintentional and _ its observation Wei and external devices for data = exchange and transmission. Silk reduction of this wealth _, can also be developed by the j group with the appropriate method, fine image, analysis and display 0 ί 2 (4) the appearance of the handheld bio-brake detection device Μ®. The hand-held biological test piece inspectors of the present invention are mainly composed of a hand-held communication device 31〇 and a darkroom device 32 that can be mounted thereon. The handheld communication device f 310 and the dark room device 32 are not built. Modules 20 and 25 are shown as shown in the second figure. Referring to Fig. 3B, the main structural composition of the hand-held biological test strip detecting device 300 of the present invention is incorporated. According to a preferred embodiment of the present invention, the hand-held communication device in the hand-held biological test strip detection device|300 supports any type of handheld communication device of 201018907 ft, including action Wei, personal number assistant ( PDA) and any known person in the field of communication will be placed on the bean 3 315 to present the detection sound in addition to the aforementioned communication: 使用 use /, he can perform the image processing algorithm of the present invention, the hand can not have communication function) 'To capture and analyze the shadow of the biological test piece to be tested, = output the corresponding detection result, / the darkroom device 320 is constructed in the earthen seat of the upper and lower outer casing 32 322, and the inclusive-image magnifying device ( For example, single-lu, module 324 and a power module milk === group 325 ^ connection, after receiving power, according to the test piece ST to be tested, a light source of different color lights is provided for complementing light. 326 is used for accommodating the biological test piece to be tested. Please refer to the fourth figure 'the test piece of the test piece shown in the third figure. The test of the hand-held biological test # detection device of the present invention On-chip and dry for carrying different types of students The test piece. For example, the design of the composite groove is carried out on the eighth piece 326, and the opening is formed, wherein the long strip == Τ ίί as the first - Α 麻 麻 织 biological test piece, hope, viral reagent (such as the detection of C-Zheyan HCV reagent) For example, 丨 槽 328 is used for accommodating - array biochip 400 'microarray wafers without biological characteristics of samples used to read antigens and antibodies Kinds of protein wafers, etc. When the biological characteristics of the sprayed sample are detected, 'microarray crystals. On-chip _\=-order has certain reaction significance, so the detection and interpretation must be determined. Detecting and analyzing, in the hand-held bio-film of the present invention = a corner forming orientation: ten, that is, the mechanical bevel 328A of the array test strip 328, which coincides with the 201018907 oblique edge 400A in the fixed direction on the biochip 400; Therefore, when the tester is placed, the test piece can be quickly oriented to release the biological test piece to the test piece carrier 326, and the third embodiment of the invention is based on the tilting biological test piece detecting device of the present invention. In the embodiment, the system utilizes the existing handheld communication device In the middle, the phase device enters the image capture of the biochip, and in the center, the unit 70 and the existing image processing module towel establish an image processing algorithm to analyze the image of the test piece. When testing the biological test piece, firstly, the hand-held communication device and the biological test piece amplifying device (that is, the darkroom device) of the pot are assembled to form a hand-held biological test position, and the test is performed. The shape of the film is directed to the test piece carrier.
接著電源模組以提供絲模之電力，如步驟 所不，其中光源模組可根據待測生物試片的型態而切換以 =不=色光進行補光，以使後續所操取之影像更利於進行灰 ，轉化處理。舉例而言，若待測生物試片係—陣列式生物晶 t則光職_提供自絲強化影像之财對比，提高判讀 性；另—方面，料測生物試片係、—長條形生物試片， ^其控觀制爾—般係魏紅色之_，故光源模組係 j但不限於）、縣或紫光轉行補光之用，明加影像的 ϋ異。在本發财，絲色光的選擇係配合生物試片所採 色素呈色（colorimetric；)技術而定，針對不同型態不同呈 的待;!彳生物試片而提供不同顏色的光源，以強化影像之對 比0 為提供後續影像解析的正確性，在本發明之一較佳實施例 係利用影像放大裝置（如放大鏡、聚焦透鏡組等）放大待 晶片之影像’如步驟520所示；接著以影像擷取模組(例 .手持式通訊裝置之照相模組）擷取經放大之待測生物晶片 201018907 進之：之:將理影Then, the power module provides the power of the wire mold, as in the step, wherein the light source module can switch according to the type of the biological test piece to be tested to fill the light with no = color light, so that the image taken later is more Conducive to ash, conversion treatment. For example, if the biological test piece to be tested is an array of biocrystals t, then the ray of light will provide a comparison of the wealth of the image enhanced by the silk, and improve the interpretability; on the other hand, the biological test piece system, the long strip creature The test piece, ^ its control system - the general system of Wei red, so the light source module is not limited to), county or purple light to fill the light, Mingjia image is different. In this fortune, the choice of silk color is based on the colorimetric technology of the biological test piece, and it is different for different types; the biological test piece provides different color light sources to strengthen Comparison of images 0 In order to provide correctness of subsequent image analysis, in a preferred embodiment of the present invention, an image magnifying device (such as a magnifying glass, a focusing lens group, etc.) is used to enlarge the image of the wafer to be as shown in step 520; The image capture module (for example, the camera module of the handheld communication device) captures the enlarged biometric wafer to be tested 201018907 into:
€像，=====解::無： ί::像壓縮效率，在步驟544中，係對影像進行色_ί: 像區域處 =素轉換為-像素’優化二影 接著由影像解析單元進行最佳化值運算 你t步驟550所示。在此步驟中’係利用統計^法對 算爾得最朗健來進行二值化處理而 階2==黑白模式’並將每一像素以0〜255級之不同灰 生物投影演算法並配合賴化定位演算（陣列式 生物曰曰片）或百分蚊位演算（長條形生物試片），即可透過 區域比例_对时駭位出_式生物⑼與長條形生 物試片之待測影像，以確認一分析區域，如步驟552所示。舉 例而言，在_式生物“巾，可透過上财絲確認分析區 域中之標關闕行》懷’以進行錄彳粮或基目型態之比 對；另一方面，而長條形生物試片檢測中，亦可透過上^方式 來確認試片上分析區域（如第一 A圖中所示之受測區域D) 中多條4示的測s式線之位置順序及其顏色深淺，以續認標的反應 是否發生以及其相對反應強度。 在本發明中，由於待測生物試片之影像係經灰階化處理， 故可透過分析區域灰階值之比較而得知相對反應強度，以得標 的反應之半定量分析結果，如步驟554所示。舉例而言，由於 在長條形生物試片中，控制線具有固定的呈色濃度，故於影像 201018907 經灰階化處理後，其灰階值係同樣為固定 影像之灰階值的比較，即可得知 透過月豕 相對反應顧 腐娜嫩_她，、嘯 =後 且輸出影像解析雜(例如基因型解讀 即結束本次檢測。千疋里刀狀果’如步驟娜所示， 在本發明之-較佳實施例中，上述影像片 輸出而顯示於手持式通訊裝^的 透過其他傳輸方式（例如傳輸線、纟 設備、檢測人員或醫師處）以供後續分析或監= 如用物處理 置及發贿獅之顿式生械片檢測裝 置及其方法具有局靈敏度、檢測方便等優勢，且其與現廣泛並 夺式ΐ訊裝置相容，可輕易與手持式通訊裝置搭載而進 =隨身、即時且準確的各式生物試片半定量檢測。此外，由於 本發明之檢測方法並非使用與龐大資料庫進行比對之方式，因 易載人手持裝置之處理模組中，無須耗費大量記憶體與 時間來進硫猶果的比對’且可_非_職點之影像。 因此，相較於現有技術者，本發明實一新穎、進步且且 用性與競爭性之發明，深具發展價值。 八一 在詳細說明本發明的較佳實施例之後，熟悉該項技術人士 y清楚的瞭解，在不脫離下述申請專利範圍與精神下進行各種 變化與改變’且本發明亦不受限於說明書中所舉實施例的 方式。 【圖式簡單說明】 12 201018907 第一A圖係一長條形生物試片之外觀示意圖； ，一B圖係一陣列式生物基因型晶片之外觀示奇圖 圖係—陣列式生物蛋白質晶片之外觀示i圖；’ 第-圖係-模組方制，魏雜據本翻 試片檢測裝置之一較佳實施例； 于飞生物 第三A圖係本發明之手持式生物試片檢測裝置之一較佳 實施例之外觀示意圖； 第三B ®係本㈣之手航生物試諸職置之 實施例之結構組成示意圖； 第四圖係本發明之手持式生物試片檢測裝置中試片載且 之外觀示意圖；及 、 第五圖係-流糊’其說明本發明之手持式生物試片檢測 裝置的運作方法步驟。 【主要元件符號說明】€ like, ===== Solution:: None: ί:: Like compression efficiency, in step 544, the image is colored _ί: Image area = prime conversion to - pixel 'optimal two shadows and then image resolution The unit performs an optimization value operation as shown in step 550. In this step, 'Using the statistical ^ method to calculate the most robust to binarization and the order 2 == black and white mode' and each pixel with 0 to 255 levels of different gray biological projection algorithm and match Laihua's positional calculus (array biofilm) or percent mosquito bite calculus (long strip biological test piece), which can pass through the regional ratio _ 骇 骇 _ _ _ _ _ _ _ _ _ _ _ _ _ The image to be tested is used to confirm an analysis area, as shown in step 552. For example, in the _ type of biological "canvas, you can confirm the mark in the analysis area through the upper wire to carry out the comparison of the grain or the base type; on the other hand, the long strip In the biological test piece detection, it is also possible to confirm the positional order and the color depth of the plurality of four measured s-shaped lines in the analysis area (such as the measured area D shown in the first A picture) on the test piece by the upper method. In the present invention, since the image of the biological test piece to be tested is subjected to gray scale treatment, the relative reaction intensity can be known by comparing the gray scale values of the analysis area. The results of the semi-quantitative analysis of the obtained reaction are shown in step 554. For example, since the control line has a fixed color concentration in the strip-shaped biological test piece, after the grayscale treatment of the image 201018907 The grayscale value is also a comparison of the grayscale values of the fixed image, and it can be known that the relative response through the lunar sputum is nurturing _ her, whistling = and the output image is parsed (for example, genotype interpretation is the end) Sub-test. Millennium knife-shaped fruit' In the preferred embodiment of the present invention, the video chip is outputted and displayed on the handheld communication device through other transmission methods (such as transmission lines, devices, inspectors, or physicians) for subsequent analysis. Or supervision = such as the use of processing and bribery lion's ton-type bio-mechanical film detection device and its method has the advantages of local sensitivity, easy detection, and it is compatible with the current widely-accepted device, can be easily handheld The type of communication device is equipped with semi-quantitative detection of various biological test strips that are portable, instant and accurate. Moreover, since the detection method of the present invention is not used in comparison with a large database, the handling of the portable human hand-held device In the module, it is not necessary to consume a large amount of memory and time to compare the sulfur and the fruit, and the image can be compared with the prior art. Therefore, the present invention is novel, advanced and useful compared with the prior art. And the competitive invention, which has great development value. After the detailed description of the preferred embodiment of the present invention, it is clear to those skilled in the art that the patent application scope is not deviated from the following claims. Various changes and modifications are made in the spirit of the present invention, and the present invention is not limited to the embodiment shown in the specification. [Simple description of the drawing] 12 201018907 The first A picture is a schematic view of the appearance of a long strip biological test piece; , B-picture is an array of bio-genetic wafers, the appearance of the singular map system - the appearance of the array of bio-protein wafers i diagram; 'the first-picture system-module system, Wei miscellaneous according to the test strip test A preferred embodiment of the device; Figure 3 of the present invention is a schematic view of a preferred embodiment of the hand-held biological test strip detecting device of the present invention; the third B ® is the hand-held biological test of the fourth (4) BRIEF DESCRIPTION OF THE DRAWINGS FIG. 4 is a schematic view showing the appearance of a test piece contained in the hand-held biological test piece detecting device of the present invention; and FIG. 5 is a flow chart showing the hand-held creature of the present invention. The method steps of the test piece detecting device. [Main component symbol description]
100 長條形生物試片 105 圓點陣列式生物晶片 110 作用區 115 基底 120 控制線 125a、125b 測試點 130 測試線 20、25 模組 201 中央處理單元 202 影像操取模組 203 影像處理模組 204 輸出模組 205 通訊模組 13 201018907100 strip biological test piece 105 dot array biochip 110 active area 115 base 120 control line 125a, 125b test point 130 test line 20, 25 module 201 central processing unit 202 image manipulation module 203 image processing module 204 output module 205 communication module 13 201018907
251 試片載具 252 影像放大單元 253 光源模組 254 電源模組 2031 前置處理單元 2032 影像解析單元 300 手持式生物試片檢測裝置 310 手持式通訊裝置 315 顯示面板 320 生物試片分析裝置 321 > 322 外殼 323 影像放大裝置 324 光源模組 325 電源模組 326 試片載具 327 長條形試片槽 328、328A 陣列式試片槽 400、400A 陣列式生物晶片 500 方法 510〜560 步驟 14251 Test strip carrier 252 Image enlargement unit 253 Light source module 254 Power module 2031 Pre-processing unit 2032 Image analysis unit 300 Hand-held biological test strip detection device 310 Hand-held communication device 315 Display panel 320 Biological test strip analysis device 321 > 322 Housing 323 Image Amplifying Device 324 Light Source Module 325 Power Module 326 Test Strip Carrier 327 Long Strip Test Strips 328, 328A Array Test Strips 400, 400A Array Biochip 500 Method 510~560 Step 14
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| TW (1) | TW201018907A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI490476B (en) * | 2013-08-08 | 2015-07-01 | Ixensor Inc | Method and apparatus for analyte measurement |
| TWI570403B (en) * | 2013-08-08 | 2017-02-11 | 安盛生科股份有限公司 | Method and apparatus for analyte measurement |
| US9600878B2 (en) | 2012-04-06 | 2017-03-21 | Ixensor Inc. | Reading test strip with reaction area, color calibration area, and temperature calibration area |
| US9658217B2 (en) | 2014-02-17 | 2017-05-23 | Ixensor Co., Ltd | Measuring physical and biochemical parameters with mobile devices |
| US9778200B2 (en) | 2012-12-18 | 2017-10-03 | Ixensor Co., Ltd. | Method and apparatus for analyte measurement |
| TWI640762B (en) * | 2017-09-04 | 2018-11-11 | 東吳大學 | Portable fluorescent detector combined with mobile device |
-
2008
- 2008-11-05 TW TW97142646A patent/TW201018907A/en unknown
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9600878B2 (en) | 2012-04-06 | 2017-03-21 | Ixensor Inc. | Reading test strip with reaction area, color calibration area, and temperature calibration area |
| US9778200B2 (en) | 2012-12-18 | 2017-10-03 | Ixensor Co., Ltd. | Method and apparatus for analyte measurement |
| US10921259B2 (en) | 2012-12-18 | 2021-02-16 | Ixensor Co., Ltd. | Method and apparatus for analyte measurement |
| TWI490476B (en) * | 2013-08-08 | 2015-07-01 | Ixensor Inc | Method and apparatus for analyte measurement |
| TWI570403B (en) * | 2013-08-08 | 2017-02-11 | 安盛生科股份有限公司 | Method and apparatus for analyte measurement |
| US9658217B2 (en) | 2014-02-17 | 2017-05-23 | Ixensor Co., Ltd | Measuring physical and biochemical parameters with mobile devices |
| US10690659B2 (en) | 2014-02-17 | 2020-06-23 | Ixensor Co., Ltd. | Measuring physical and biochemical parameters with mobile devices |
| TWI640762B (en) * | 2017-09-04 | 2018-11-11 | 東吳大學 | Portable fluorescent detector combined with mobile device |
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