TW200901954A - Antiviral composition - Google Patents

Abstract

The present invention provides an antiviral composition having high antiviral activity and low side effect (cell toxicity). The antiviral composition contains at least one component selected from the group comprising 5, 7, 4'-trihydroxy-3', 5'-dimethoxyflavone, 3-hydroxypyridine, p-hydroxybenzaldehyde and vanillin as an active component.

Description

200901954 - IX. Description of the invention: • [Technical field to which the invention pertains] - The present invention relates to an antiviral agent. [Prior Art] The virus system is incapable of self-propagation, and the parasitic proliferative animal or plant micro-pathogen can be classified into animal virus, plant virus, and microbial virus. Human cytomegalovirus is highly specific and does not proliferate in cells other than human-derived cells. For proliferation, various primary cultured cells such as human fetal skin, muscle, foreskin, and lung, or established cells such as MRC-5 and WI-38 can be used. An intranuclear inclusion body (intestinal) and a cytoplasmic inclusion body can be confirmed in the infected cells. Transplacental infection (vertical infection) is the cause of stillbirth, premature birth, and causes congenital infection (CID, also known as cytomegalic inclusion disease, giant cell inclusion body disease). It is also considered as an acquired infection. At this time, the virus that is latently infected is generally susceptible to infection due to immunosuppressive therapy, steroid use, and AIDS or cancer patients after inapparent infection. The sexual host is activated, causing severe opportunistic infections such as pneumonia, retinitis, and colitis. Antiviral agents for the prevention and treatment of diseases caused by this specialized virus are under development. For example, Ganciclovir (i.e., GCV), Cidofovir (i.e., CDV), foscarnet (i.e., PFA), and Fomivirsen are known. Among them, GCV is widely used, 5 320044% 200901954 • However, in recent years, the GCV-resistant virus system has become a problem. In addition, there is also a problem of strong (cytotoxicity) in the side effects, so there is no need for side effects and there is a demand for a drug-resistant virus-effective antiviral activity.

The influenza virus (also known as flu virus) is a virus that infects humans and causes influenza infection, and has type A, type B, and type C. In addition, some of the influenza viruses infect chickens and other poultry and cause highly pathogenic avian influenza (also known as chicken mites), both fatal and legally infectious, causing great damage to the chicken industry. In 2006, the anti-avian flu drugs used were three types: amantadine, Zanamivir, and Oseltamivir (trade name Tamiflu). On the other hand, amantadine-resistant influenza virus, zanamivir (osexetvir)-resistant influenza virus, and zanamivir and oseltamivir for these agents The emergence of a drug-resistant virus has also been reported. Therefore, a drug system having no side effects and having antiviral κ. activity which is also effective against a drug-resistant virus is in demand. On the other hand, the Bamboo grass extract is known to have antibacterial properties. For example, the antibacterial effect of Staphylococcus aureus, Pdeudomonas aeruginosa, and Escherichia coli against bacteria belonging to the cause of traumatic infection has been reported (Patent Document 1 and Patent Document 2) ), or the antibacterial effect of Helicobacter pylori, which is considered to be a cause of gastric ulcer. After the war, it was found in the animal experiment that Sasa veitchii has an inhibitory activity against the liver cancer of the old 6 320044 200901954 - and many studies have been carried out in the direction of carcinogenic activity (Non-Patent Document 1). In addition, research has been conducted on the superior-different anti-corrosion effect (Non-Patent Document 2) and the antibacterial effect (Non-Patent Document 3) of H. chinensis. However, most of these studies are only organic acid analysis based on gas chromatography, and there are few reports on the separation and purification of the antibacterial effect body. It is also known that coumaric acid and its derivatives are antibacterial to Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa (Patent Document 3). In addition, it is also known that the extract of the white bamboo contains coumaric acid, ferulic acid, coffeic acid, vanillin, and the like. These mixtures are antibacterial to Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa (Patent Document 4). However, the details of the components in the extract of the white bamboo extract and their antiviral activity are still unknown. [Patent Document 1] WO00/067707 [Patent Document 2] JP-A-2003-201247 [Patent Document 3] Japanese Laid-Open Patent Publication No. 2004-359626 [Patent Document 4] 曰本开开 2006-36731 [Non-Patent Document 1] M Shibata, K. Kubo, M. Onoda, Folia Pharmacol. Jpn, 72, 531-541 (1976) [Non-Patent Document 2] NV Chuyen, T_ Kurata, H. Kato, J. Antibact. Antifung. Agents, 11, 69-75 (1983) [Non-Patent Document 3] NV Chuyen, H. Kato, Agric. Biol. Chem., 46, 2795-2801 (1982) 3-128 (2004) 7 320044 200901954 [Summary of the Invention] Problem to be solved) Ben: The purpose of Ming is to provide antiviral agents. Main 2; Month: The second objective is to provide an antiviral agent with high antiviral activity and low side effects (cell sputum). (Method for Solving the Problem) The present invention provides an antiviral agent as follows: (1) A: an antiviral agent is selected from the group consisting of 5, 7, 4, -trihydroxy-3,5,-dimethoxy. At least one of a group consisting of flavonoids, 3-hydroxypyridine, p-hydroxybenzaldehyde, and vanillin is used as an active ingredient. (7) An antiviral agent which is quinone 5,7,4,-trihydroxy-3,5,-dimethoxyxanone or 3-hydroxypyridine as an active ingredient. (3) An antiviral agent which is quinone 5,7,4,-trihydroxy-3,5,-dimethoxyxanone as an active ingredient. (4) The antiviral agent according to any one of the above (1) to (3) wherein the virus is a herpesvirus. (5) The antiviral agent according to any one of the above (1) to (3) wherein the virus is a cytomegalovirus. (6) The antiviral agent according to any one of the above (1) to (3) wherein the virus is a human cytomegalovirus. (7) The antiviral agent according to (3) above, wherein the virus is an influenza virus. (8) The antiviral agent according to (7) above, wherein the virus is an influenza A virus or a influenza B virus. (9) The antiviral agent according to (7) above, wherein the virus is avian influenza virus. 320044 8 200901954 The "virus" in the present invention includes animal virus, insect virus, plant virus, bacterial virus (bacteria), and X refers to an adverse effect on animals and plants including humans, including blacks and the like. virus. The virus of the present invention 2 may be particularly effective, and examples thereof include herpes virus and influenza virus, and particularly, for example, cytomegalovirus, particularly human cytomegalovirus, monkey cytomegalovirus, mouse cytomegalovirus, etc. Cytomegalovirus; human influenza virus such as influenza A virus, influenza B virus, influenza C virus, or avian influenza virus. (Effect of the Invention) The antiviral agent of the present invention is useful for various viruses, in particular, a running virus, particularly a cytomegalovirus, more particularly a human cytomegalovirus; an influenza virus, particularly an influenza A virus, and an influenza B virus. The influenza C virus or the avian influenza virus has a high antiviral activity. The antiviral agent of the present invention has the advantage of low cytotoxicity. [Embodiment] ▲ In order to clarify the antibacterial effect of Sasa veitchii and specify its antibacterial active ingredient, the inventors of the present invention extracted the water from the leaves of the white bamboo leaves in various (four) frames, by various means. Means for separating the coats and investigating the antiviral activity of each component. As a result, it was found that 5,7,4 is called ","flavonoid", 3_base biting, p-benzoic acid: disaccharide: cheese, which is 5j,4,_trihydroxy_3 ,, 5,, -dimethoxy, and 3-carbyl. Specific bite, especially 5,7,4,_trisyl group_3,,5,, _ meimei (also known as flavonoid) shows excellent antiviral activity against various viruses, 320044 9 200901954 and completed the present invention . Hereinafter, the present invention will be specifically described. The active ingredient of the invention 5,7,4'-trihydroxy-3',5,'-dimethoxyflavone, 3-hydroxypyridine, p-hydroxybenzaldehyde, and vanillin are extracted from the mountain white bamboo Out, but of course it can be a synthetic or a source from other sources. For example, plants other than white bamboo, especially most plants belonging to the family Poaceae, such as rice (rice), wheat, maize, barley, semale, sugar cane, bamboo, Miscanthus, pampas The leaves or stems of grasses such as grass and Phragmites contain a large amount of 5,7,4'-trisyl-3',5,'-dimethoxyflavone (also known as flavonoid). Extract from it. The specific plant names are as follows: rice, wheat, barley, oats, rye, panicum miliaceum, setaria italica, eucalyptus (Echinochloa esculenta), maize, Eleusine coracana, sorghum ( Sorghum bicolor), bamboo, Zizanialatifolia, sugar cane, ma-yuen, reed, miscanthus, bamboo grass, Arundo donax, pampas grass, Zoysia japonica. The leaves, stems, and the like of the above plants may be extracted with an appropriate solvent, and the flavonoids may be isolated and purified by separation and purification means such as HPLC. For example, the aqueous extract can be concentrated, and the solid matter is extracted with an alcohol, an aqueous alcohol (for example, decyl alcohol, ethanol, aqueous sterol, aqueous ethanol), and the solid is dissolved in water by using ethyl acetate. It can be refined by distribution or the like. The above compounds (vanillin, p-hydroxybenzaldehyde, 3-hydroxypyridine, 5,7,4'-trihydroxy-3',5,'-dimethoxyflavone) (also referred to herein as 10 in this specification) 320044 200901954 : The composition of the present invention as an active ingredient: 'wide': "used". For example, it is suitable as an antiviral agent such as a mucous membrane retention device. The antiseptic agent and the antiviral agent of the present invention are any one of a liquid form, a colloidal form, a colloidal form and an aerosol form. The present invention: the anti-logic agent can be administered orally, and administered orally. Any of the two oral administration forms may be exemplified by _, pills, powders, liquids, S-sugars, candy, chocolate candy, bread, biscuits, buckwheat beverages, fresh foods or beverage aqueous solutions, seasonings, and the like. : 'Two-injection type of production line can be listed as injection, topical administration (cream: agent, intravaginal administration type (filling cotton ball (_«) 1: «:::; ^ ··-- / 叩 媸 媸 3 times of the invention anti-virus = red cosmetics or other types of cosmetics (emulsion, 匕 ^ (four) cream (10) called) or bath Containing the present invention, the two S = 叩成成's made Wu Rong liquid, shampoo, shower gel half: two or liquid type, etc. can also be made into = type of solid fog agent. In the treatment of pharyngeal head inflammation, the antiviral agent of the present invention is an antiviral agent used for mammals, urchins, fish and shellfish, cockroaches, and other insects, in addition to humans, and mites. The two antiviral agents can be used as r for such animals: therefore, the present invention has several diseases and μ (for example, pet medicine 320044 11 200901954 medicine, animal feed, pet food, etc.) ^ The poison is effective for animals. In addition, V, the antiviral agent of the present invention is also used as a preservative for the disease-resistant tablets J as various plants. To make the various dosage forms of the present invention, For the disease-resistant agent, in addition to the predetermined amount of δ-δ, you can also use the Tong Dian Dian #± * a , η .^ , the hanging i composition, cosmetics, skin, and used in the temple. Oily rot, etc. Knife 4 base material, moisturizer, waterproof, etc. Antiviral The water used in the medium is not particularly limited. However, high-purity water such as tap water, natural water, and ion-exchanged water can be used as an oily component, such as M, butter, lard, horse oil, and wool. Fat, clear animal oil; olive oil, grape seed oil, palm: oil (such as rice germ oil), sesame oil, vegetable oil, safflower oil, salad t plant oil; mobile stone butterfly, higher fatty acid ester (For example, citric acid palmitate, isopropyl gluconate, meat beans, 丄Π 寇 寇 寇 octyl octyl decyl ester), synthetic oils such as polyoxygenated oil, semi-synthetic oil. The knife can be used in combination with the protection of the skin, the effect of imparting emollient properties (the surface of the skin is covered with a film, the effect of softness and elasticity at the same time as the prevention of money), and the dry feeling. A combination of sulphate, eucalyptus oil and octyldodecyl pentanoate is one of the preferred examples. In order to adjust the hardness and the flow name of the antiviral agent, a solid oil such as stearic acid, stearyl alcohol, behenic acid, cetyl alcohol (Cetan〇1), petrolatum or the like may be used, preferably Stearic acid and cetyl alcohol are used in combination. In order to manufacture the antiviral agent of the present invention into a cream composition, 320044 12 200901954 can be used. The compound of the present invention, water, ?q, *, *, A, 4 into a milk-like cream Chemical agent. : ^ Sub-fan limitation 'Generally, a single emulsion type glyceryl monostearate (also known as η self-oxidizing agent) is used in combination. Adding milk to early heart acid (4) The antiviral agent of the present invention can also be used together with other antiviral agents. In the antiviral, sputum, & 7 ^ _ agent, moisturizer, wounded household (four) ^ requirements of the present invention may additionally contain stability one -. 4, preservatives, surfactants, etc.

The grouping agent and Λ' can be exemplified by a slower ethyl polymer and potassium hydroxide d.] mouth diethyl-alcohol distearate ((polyethylene giycoI glyclTl? ^ 〇 ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ (polyethylene - alcohol single hard two:: ame) (that is, polyethylene glycol tartaric acid vinegar and polyethylene _ _ early hard moon vinegar, 2 to 2), ()) The molecular weight of polyethylene glycol is 1, and 〇〇〇 is 9, its stability is high, and water and oil are not separated, and 2:2 can effectively adjust the hardness when applied to the skin, so it is preferable. (especially from the root of Mu Lijun; glass to take urine;; 1 collagen, Lu Chong extract B s Lu will be a stroke (2) is better), urea, 1,3-two sea steamed sugar a), Yamanashi Alcohol, amino acid... Licorice =! For the cure of the sword, for example, allantoin, diammonium glycyrrhizinate, early pregnancy, wormwood extract, etc. Preservatives are auxiliary materials. Benzene f, 对 Μ Μ 储 储 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例 例Sodium propionate, mixed fat Ester (glyceryl caprate, lauric acid polyglyceride-2, lauric acid polyglycerol _ 丨〇 mixture), phenoxyethanol, photoreceptor No. 201 (yellow pigment), hydrazine, 2 y The diol is preferably used in the form of p-parabenbenzoic acid, mixed fatty acid vinegar, and 1,2-pentanediol. In the case of a surfactant, it may be exemplified by N-mercapto-L- faceamine. Sodium, polyoxyethylene ethyl sorbitan monostearate, etc., if necessary, may also contain aroma components, such as orange oil, lemon oil spruce oil, spices, etc. Containing the antiviral agent of the present invention The mucosal protective composition, the virus infection prevention and/or therapeutic composition can effectively inhibit the virus from invading the mucous membrane or the wound site and the skin of the eyes, nose, throat, ears, anus, genitals, etc., and effectively prevent m Treatment includes viral infections of nosocomial infections. More specific forms of viral infections and/or therapeutic compositions are exemplified by mucosal protective cloths or oral compositions. Mucosal protective fabrics are ventilated by impregnation and spraying. Seven of the 脰 (such as silk, cotton, hemp and other natural Dimensional; y: ethane), polyethylene, polypropylene, nylon, polyester, acrylic acid, fiber, and other two or more mixed cloths; = for convenience, in this specification "(4) 1 and: also includes the fiber itself, thread, paper, etc. Menstruation: Wei = type Ϊ: words, except gauze, mask, eye mask, menstrual belt fine ▼, toilet paper, gauze for the treatment of sore, earplugs, 320044] 4 200901954 • Liquid _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Articles such as articles, sheets, quilt covers, pillow covers, bed supplies, etc., interior decorations such as curtains, wallpapers, carpets, interior decoration materials, medical materials such as surgical sutures, etc., which directly or indirectly contact the skin. Examples of the oral composition containing the antiviral agent of the present invention include soft gelatinous (y) face y), bed kick (jelly), gradual dissolution tablet (tr〇che), candy (candy/chewing gum, chocolate candy, lozenge, Pills, mouthwashes, mouthwashes, toothpastes, films attached to mucous membranes, sprays for the treatment of pharyngeal head inflammation, etc. The antiviral agent of the present invention has high antiviral activity, and the compound of the present invention is only in the range of (4) to 1 ppm. The anti-viral activity can be exhibited by the concentration of the solids on the left and right. When the antiviral agent of the present invention is used in humans, it is considered that the symptoms, age, and body weight of the adult are from i to 5, preferably from 5 to 2000 mg per day. More preferably, it can be administered from about 1 to about 1 inch. ...-People or sputum is divided into 2 to 6: if it is to be made, for example, soft rubber, east gum, gradual dissolution lozenge, candy, chewing gum, chocolate candy, ingot Agent, pill (such as Rendan), mouthwash, mouthwash, tooth-poor, film attached to the mucous membrane, 箄 脉 丄 丄 4 thick film 4 mouth ^ composition containing the compound of the two St cavity composition of the present invention In the stage, in order to make the present invention: the above-mentioned dosage, the appropriate amount is added to the oral cavity In the raw material, the solid matter of the compound of the second= is preferably added...called Berry 0 or so, more preferably 6 to 15 Χ (1Π 1〇-5)% by mass, 320044 15 200901954 Best 8 to 12XO0-1 to ΙΟ · 5) About % by mass. The dosage form of the antiviral agent of the present invention may, for example, be a capsule, a dry syrup, a tablet, a pill, a powder, a liquid, such as a liquid or gel for nasal spray, an oral spray, or the like. It is suitable for percutaneous absorption of nasal preparations, ointments, emulsions and emulsions, such as nasal sprays and pharyngeal heads. These can be modulated according to the usual method. The base component for preparing the antiviral agent of the above dosage form may, for example, be an excipient such as glucose, lactose, Yan sugar, water preparation, dextrin, cyclodextrin, and temple powder; gum arabic, methylidene fiber Bonding agents such as sodium, crystalline cellulose, gmn base; disintegrants such as starch; magnesium stearate, axe = (4) sour cool # lubricant; spices, chlorophyll, mint, ι•Menthol, etc. High-alcohol such as octyl dodecyl alcohol; fatty acid vinegar such as isopropyl vinegar, adipic acid, isopropyl vinegar and isopropyl vinegar; polysorbate ribs and polyemulsion Base-hardening castor oil and other suspending agents; slow-growth polymer, propyl cellulose, polyvinyl ketone ketone and sodium hyaluronic acid and other macromolecules... glycerol propanol and butyl glycol; Diisopropanolamine, guanidine reduction, Wei Dihydrogen and guanidine hydrochloride, etc.; chlorate citrate, sodium oxide, sodium thiosulfate, sodium sulfite and sodium edetate so^um edetate) Anteinizing agent; p-parabenzic acid A from the purpose of 'the passage;! V^S^SI ' ^^^^^(benzalkonium chloride)^ this = right to (be Preservatives such as nzeih〇nium chl〇Hde). When the antiviral agent of the present invention is used in the case of the mold of the present invention, the protective cloth (gauze, etc.) of the mucosa or the wound site may be replaced by the use of the present invention for about 1 to 3 times. When used in the form of surgical suture 320044 16 200901954, the virus can be effectively inhibited from invading the affected part to promote the recovery of the surgical site. When the antiviral agent of the present invention is used in a form of a protective cloth in order to prevent infection in a hospital such as a hospital, the anti-viral agent of the present invention can have an infection preventing effect for a long period of time. When the effect is lowered by washing, the protective cloth may be appropriately replaced or the protective cloth may be further contained in the compound of the present invention. When the antiviral agent of the present invention is used as an oral composition, it is extremely easy to prevent viral infection and inhibit virus proliferation by appropriately preliminarily containing it in the mouth. Further, it is advantageous that the composition of a sustained-release type such as chewing gum or candy can exhibit an effect for a long period of time. The antiviral agent of the present invention can also be used in the form of an emulsion or an oil. It is extremely easy to prevent viral infection and inhibit virus proliferation by previously applying an emulsion or oil containing the compound of the present invention in an amount of 2 to 2 (1 (10-1 to 10_5)% by mass of the solid matter to the skin or the like. Filtration device The present invention also provides an air filtration device containing the compound of the present invention as an active ingredient. In a specific form, it can be exemplified, for example, for use in a ventilation fan, an air conditioner, an automobile air conditioner, an air intake, an air exhaust. Filters for air passage parts such as outlets, screens, air cleaners, etc. There is no particular limitation on the basis of the filter. 5, for example, natural fibers such as silk, cotton, wool, and hemp; polyamine phthalate, polyethylene, Synthetic fibers such as polypropylene, nylon, polyester, acrylic, etc.; semi-synthetic fibers; or woven fabrics, fabrics, non-woven fabrics, papers, etc. of a mixture of two or more of these, as long as by impregnation, spray 17 320044 200901954 .: And the like: and the filter containing the compound of the present invention is diluted with water. The content of the compound of the present invention in the filter, • the solid content is preferably 2 to 20 χΠ ο - Stone 1Λ 5 5 , (1〇 to 10)% by mass, more preferably ό to l)x(10 main; [〇5) 詈%, 曰*%%. ) 1 / 〇 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 佳 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 The method of investigating the antiviral effect of the mountain white. Method: Infecting human cytomegalovirus=MV with human porphyry fibroblast (MRC_5) cultured in a single culture dish in a culture dish; And ganciclovir (G_ici called resistance = sex strain R), adding a commercially available mountain white bamboo extract tTWEBS (solid content concentration%% by mass)) diluted aqueous solution = / Chendu from the mountain white bamboo extract separation The purified product and the culture solution containing the municipal cockroach were started to be cultured in a 5% carbon dioxide incubator. ° (1) On the first day, the third day, and the sixth day after the culture, the effect of the cytopathic effeci (ie, y) was observed by a microscope to evaluate the effect of each sample on CPE. (7) The effect of each sample on the virus production was evaluated by cultivating (4) the number of the disease records of the cultivating liquid wipes at 6 days. (7) After training! On days, 3rd, and 6th, each dyed cell was dissolved in a liquid returning liquid m-filament protein j 320044 18 200901954 - The performance of viral proteins was compared by Western blotting. In particular, the needle--important early-early protein (immediate_earIy pr〇tein), which functions in the early days after infection and which is the trigger protein (8) gger protein, which plays a role in viral recognition, and the occupational protein, etc. The performance of late proteins is compared to (10) the effect of each sample. The cytopathic effect in the case of using a dilute aqueous solution of the extract of the white bamboo extract (twebs sold by the phoenix hall (solid matter 50% by mass)) is shown in Fig. 1 and the virus red (four) filament is shown in Fig. 2. For both of the τ〇·6 strain and the 93-m strain, it was confirmed that at least %4% of the concentration inhibited the cytopathic effect and the virus proliferation effect. This fact indicates that the present invention firstly found that the components (especially flavonoids) in the extract of S. chinensis are effective not only for the usual cytomegalovirus, but also for the virulence virus. Run a rash, hi... silk! The refined product of the separation of the white bamboo and bamboo shoots was made using p-coumaric acid (system=1), vanilla (series 2), p-benzopyrene plate (series 3), and 3_light base tons ( Series 4), 5,7,4 -di-based _3,,5,,-dimethoxyxanthine, ie, flavonoids) (Series 5). The HCMV proliferation inhibitory effect of each compound is shown in Fig. 3. In addition, the relationship between the same concentration of wheat scutellariae and the viral power price (vί·份) is not shown in Fig. 4. It can be seen that when the concentration of flavonol is 111//g/ml, the proliferation of the virus is almost completely suppressed. Further, 1 (: (50% inhibitory concentration) (cell) (g/ml), EC50 (50% effective concentration) (from g/ml), and § 1 (selection index) of various compounds are shown in Table 1. 320044 19 200901954 [Table i] Compound ic5〇ec50 SI p-coumaric acid 187 8.2 22.8 Vanillin 215 48.0 4.5 p-Hydroxybenzaldehyde 305 12.8 23.8 3 - ratio σ 263 3.4 77.4 Flavonoid 2050 1.7 1205.8 Gemcyclovir 0.3 to 0.8 According to Table 1, the compounds of the present invention, especially flavonoids (flavonoid derivatives), have a significantly low cytotoxicity and a low effective concentration (high activity). Therefore, the selection index (ic5〇/ec5()) was significantly higher. Example 2 (Antiviral effect of wheat scutellariae against influenza virus) The antiviral effect of flavonoids against influenza virus was investigated according to the following method: Cell: MDCK cell influenza virus Type: A/Hiroshima/52/2005, H3N2 B/Malaysia/2506 /2004 After each virus was propagated to MDCK cells and the viral power was determined, it was stored frozen in a freezer at -80 ° C, and used after thawing at the time of use. Method: According to the usual plaque method, The MDCK cells were cultured in a plastic culture medium in a 5% carbon dioxide incubator, and the viruses were adsorbed for 1 hour at room temperature. After removing the unadsorbed virus, the pancreas containing various concentrations of flavonoids was added thereto. 0.6% acacia medium of trypsin, solidified at room temperature, and then cultured in an incubator for 2 days with 5% carbon dioxide. 20 320044 200901954 * After the culture is completed, 'to make the cells (4)' remove. After the liquid staining, count (4) the current (four) plaque number: the experiment with the addition of flavonoids, and the number of plaques (10) divided by the number of plaques (pc) of the experimental group without melamine, calculate the resistance The role of the virus (slimation inhibition rate = pt / pcxl 〇〇%). When the same concentration of wheat scutellariae is o.3 / zg / ml, for the proliferation inhibition of influenza A virus ^ ^ a 曰 straight sheep is) 2% The inhibition rate of proliferation of β-flu sputum was 48%. The results are shown in the 5th. The toppings fully show that buckwheat also shows the inhibition of proliferation of sputum against other influenza viruses such as avian flu. Formulation Example 1 (capsule) Putting flavonoids 25 〇mg into a vessel _ Taiwan & Preparation Example 2 (ingot Agent) I in the capsule, the cost of the antiviral agent of the invention.

The wheat sassafras and the yam are mixed and molded to prepare a tablet containing 100 mg of wheat scutellaria in one tablet. ^ ^ J [Simple description of the schema] [Diagram 1] Microscopic photo of the inhibition of cytopathic effect of virus-infected white bamboo extract (twebs) on virus infection ^ 2 Figure] ) For the city of virus proliferation! 1Fig. [Fig. 3] shows the separation components in the TWEBS, and shows the Maipu Na, ', dog Inhibition of human cytomegalovirus proliferation by benzyl ketone derivatives [Fig. 5] shows inhibition of proliferation of virgin virus in influenza virus 320044 21 200901954 [Description of main component symbols]

Claims (1)

200901954 • Ten, the scope of application for patents: 3 anti-disease: 'Bu is selected from 5,7,4,-trihydroxy-3,,5,,-dioxa-o, ketone 3, ketone ratio At least one of a group consisting of a bite, a benzoic benzoate disk, and a vanilla dish as an active ingredient. 2. An antiviral agent having 5, 7, 4, _3 minus _3, 5, dimethyl diketone or 3-hydroxypyridine as an active ingredient. 3. An anti-disease agent which is the active ingredient of 5, 7, 4, and tri-based _3,5,-dimethoxy yellow S. •: The antiviral agent of any of items 1 to 3 of the patent scope, wherein the disease is herpesvirus. 5. The antiviral agent according to any one of claims 1 to 3, wherein the virus is a cytomegalovirus. 6. The antiviral agent according to any one of claims 1 to 3, wherein the virus is a human cytomegalovirus. 7. The antiviral agent of claim 3, wherein the virus is an influenza virus. 8. The antiviral agent according to claim 7, wherein the virus is a type A influenza virus or a type B influenza virus. 9. The antiviral agent of claim 7, wherein the virus is an avian k virus. 23 320044