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TW200906315A - Equol-containing extract, method for production thereof, method for extraction of equol, and equol-containing food - Google Patents

Equol-containing extract, method for production thereof, method for extraction of equol, and equol-containing food Download PDF

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Publication number
TW200906315A
TW200906315A TW097122135A TW97122135A TW200906315A TW 200906315 A TW200906315 A TW 200906315A TW 097122135 A TW097122135 A TW 097122135A TW 97122135 A TW97122135 A TW 97122135A TW 200906315 A TW200906315 A TW 200906315A
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TW
Taiwan
Prior art keywords
extract
equol
weight
soybean hypocotyl
soybean
Prior art date
Application number
TW097122135A
Other languages
Chinese (zh)
Other versions
TWI473572B (en
Inventor
Michiaki Tominaga
Takao Taki
Toshiaki Matsumoto
Izumi Hanya
Takuma Imada
Kunihiko Matsuoka
Takuya Kishimoto
Yuko Uchiyama
Hiromasa Tsuneishi
Machiko Tsuji
Tania Valdes-Gonzalez
Kentaro Tadano
Kyoko Kameda
Original Assignee
Otsuka Pharma Co Ltd
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Application filed by Otsuka Pharma Co Ltd filed Critical Otsuka Pharma Co Ltd
Publication of TW200906315A publication Critical patent/TW200906315A/en
Application granted granted Critical
Publication of TWI473572B publication Critical patent/TWI473572B/en

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    • A—HUMAN NECESSITIES
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    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
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    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
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    • A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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Abstract

Disclosed is an extract produced by extracting an equol-containing valuable component from an equol-containing fermentation product of a soybean embryonic axis. Also disclosed is a method for producing the extract. An equol-containing fermentation product of a soybean embryonic axis is extracted by using an aqueous ethanol solution as an extraction solvent to extract an equol-containing valuable component from the fermentation product with a high degree of efficiency. Alternatively, an equol-containing fermentation product of a soybean embryonic axis is successively subjected to the extraction with an aqueous ethanol solution and the extract with ethanol to extract equol and glycitein with a high degree of efficiency, thereby reducing the content of a saponin that causes an unpleasant taste.

Description

200906315 九、發明說明： 【明戶rt肩t名疗3 技術領域 5 本發明係關於一種由含有雌馬酚之大豆胚軸發酵物萃 取出有用成分之含雌馬酚萃取物以及其製造方法。此外， 本發明係關於一種有效率地從雌馬盼含有物純化出高純声 雌馬紛的方法。本發明更是關於一種含雌馬盼之食品素材 以及含雌馬酚之食品。 【先前技術3 10 背景技術 大豆中所含異黃酮（大豆異黃酮；木質素黃酮 (Daidzein)、金雀素黃酮(genistein)、黃豆素黃酮(Glycitdn)) 與雌二醇結構類似，隨著與動情素受體（以下記為er)結合 而具有抗動情素作用及類動情素作用。從迄今之大豆異黃 15酮的流行病學研究及介入研究，暗示了其因抗動情素作用 而具有預防乳癌及前列腺癌等激素相關性癌症的效果且 因類動情素作用而具有改善更年期障礙、停經後之骨質疏 鬆症及而jk脂症的效果。 近年來，指出大豆異黃_之生理作用活性本體為木質 素黃綱代謝物之雌馬盼的可能性。即，已有報告指出，雖 馬盼與大豆異黃_較下，細之結合能（特別是與卿 結合)更強，朝乳房或前列腺組織等標的臟器之移動性顯著 較:（參照非專利文獻丨♦此外，於患者對照研究中，已 出乳癌、前列腺癌患者中之雌㈣產生者明顯較 200906315 少，且亦報導指出，若將大豆異黃酮對於停經後之骨密度 及脂質代謝的改善效果分為雌馬龄產生者與非產生者來進 行解析’雌馬酚產生者有明顯改善。 雌馬酚係由木質素黃酮經腸内細菌代謝而產生者，但 5雌馬紛產生能有個人差異，已有報導指出，日本人之雌馬 酚產生者的比例為約占50%。意即，曰本人中約5〇〇/0為無法 產生雌馬紛之人(雌馬酴非產生者），而此種人即使攝取大豆 或大豆加工食品’亦無法享受到基於雌馬酚作用之有用生 理效果。因此’為了使雌馬酚非產生者表現出基於雌馬酚 10作用之有用生理效果，直接攝取雌馬酚被認為是有效的。 【非專利文獻 1】Morito K，HiroseT, Kinjo J, Hirakawa T, Okawa M, Nohara T, Ogawa S, Inoue S, Muramatsu M, Masamune Y. Interaction of phytoestrogens with estrogen receptors a: and /3 . Biol Pharm Bull 24(4):351-356, 2001 15 【非專利文獻2】Maubach J, Bracke ME, Heyerick A,200906315 IX. Description of the invention: [Ming rt shoulder t-therapy 3 Technical field 5 The present invention relates to an equol-containing extract obtained by extracting a useful component from a soybean hypocotyl fermentation product containing equol and a process for producing the same. Further, the present invention relates to a method for efficiently purifying a high-purity female horse from an equator. The present invention further relates to a food material containing equuma and a food containing equol. [Prior Art 3 10 Background] Isoflavones contained in soybeans (soy isoflavones; lignin flavone (Daidzein), genistein, glycindn) are similar in structure to estradiol, with The emodin receptor (hereinafter referred to as er) binds to anti-emotional action and eosinophilic action. Epidemiological studies and interventional studies of soy-tolerant 15-ketone from the present, suggesting that it has the effect of preventing hormone-related cancers such as breast cancer and prostate cancer due to anti-alienation effects and has improved menopausal disorders due to the action of emoji. , the effect of osteoporosis after menopause and jk liposuction. In recent years, it has been pointed out that the physiologically active body of soybean is yellow is likely to be the female of the lignin xanthate metabolite. That is, it has been reported that although Mapan and soybean are different from each other, the fine binding energy (especially combined with Qing) is stronger, and the mobility of the target organs such as the breast or prostate tissue is significantly higher: (Refer to In addition, in the patient-controlled study, the number of females (4) who had developed breast cancer and prostate cancer was significantly lower than that of 200906315, and it was also reported that if soy isoflavones were used for bone mineral density and lipid metabolism after menopause. The improvement effect is divided into female horse age producers and non-producers to analyze 'the equol producers have improved significantly. The equol is produced by the metabolism of lignin flavonoids by intestinal bacteria, but 5 female horses produce energy. There are personal differences, and it has been reported that the proportion of Japanese equol producers is about 50%. That is, about 5〇〇/0 of the person is a person who cannot produce a female horse. Producer), and such a person cannot enjoy the useful physiological effects based on equol treatment even if they ingest soy or soy processed foods. Therefore, 'in order to make equol non-producers exhibit useful effects based on equol10 physiological The effect of direct ingestion of equol is considered to be effective. [Non-Patent Document 1] Morito K, HiroseT, Kinjo J, Hirakawa T, Okawa M, Nohara T, Ogawa S, Inoue S, Muramatsu M, Masamune Y. Interaction of Phytoestrogens with estrogen receptors a: and /3 . Biol Pharm Bull 24(4): 351-356, 2001 15 [Non-Patent Document 2] Maubach J, Bracke ME, Heyerick A,

Depypere HT, Serreyn RF, Mareel MM, Keukeleire DD. Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography. J Chromatography B 784:137-144, 2003 20 【非專利文獻 3 】Morton MS, Chan PSF, Cheng C,Depypere HT, Serreyn RF, Mareel MM, Keukeleire DD. Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography. J Chromatography B 784: 137-144, 2003 20 [Non-Patent Document 3] Morton MS, Chan PSF, Cheng C,

Blacklock N, Matos-Ferreira A, Abranches-Monteiro L, Correia R, Lloyd S, Griffiths K. Lignans and isoflavonoids in plasma and prostatic fluid in men : Samples from Portugal, Hong Kong, and the United Kingdom. Prostate 32:122-128, 200906315 1997 【非專利文獻4】Tammy EH, Paul DM, Paul GF，Robert D, Stephen B, Kenneth J, Ray M, Lorraine GO, Kristiina W, Holly MS, Karen JG. Long-term dietary habits affect soy 5 isoflavone metabolism and accumulation in prostatic fluid in Caucasian men. J Nutr 135:1400-1406, 2005 t 日月内】 發明之揭示 發明欲解決之課題 10 至今為止，本案發明人已發現，以產生雌馬酚之微生 物使大丑胚軸發酵而獲得之大互胚轴發酵物可作為含有雕 馬盼之食品素材。該大豆胚轴發酵物不僅只有雖馬盼，也 t有異黃酮及皂㈣源自大豆之有用成分，據此可表現出 15 W其作為機能性素材甚為有 M@ 贫酵物中源自大豆胚軸之致 敏原降低’作為低致敏原素 明人所恭相 U甚為有用。如前述，本發 月人所毛現之前述大豆胚軸 有用生理活性，且為低致Μ酵物顯不出基於含有成分之 品素材甚為有用。性，因此得知其作為機能性食 20 另一方面，前述大豆胚軸發酸板6 製造所用之大豆胚軸種類、產生雌勿中之雌馬盼含量雖依 異，多為1重量％程度。於此，若处馬盼之微生物種類等而 提高之素材，則可因應一==盼含有比例更 各種類型之含雌馬酴食品。^多樣化4，容㈣提供 迄今為止，前述大豆胚 8 200906315 軸發酵物本身並非習用公知，且 大豆胚軸發酵物萃取出包含雌㈣^有效地從前述 未明朗化。 3用成分，此點亦尚 再者，藉前述大豆胜轴發酵物之 含有物相較於化學合成方法且 广錢㈣雌馬盼 “的優點。然而，以發酵法獲 、… 雌馬盼以外之代謝產物，亦殘存㈣含有物亦包含 此外，依發酵所用原料種類而:有::::之多種成分。 物也可能^賴歧敏原之物所伽馬粉含有 10 15 20 因此，若欲將雌馬酚 ^扣及樂σσ所使用之添加劑來一 雌馬紛之技術，出高純度純化雌_ 或缺的。但是，至目前為止 有支術亦疋不° 方法被報告出，因此，需要確立=於純化_之 效且簡便地純㈣馬_高純度的技術。王業應用、7有 咬另卜⑴述大絲轴發酵物兼具有源自大ΐ胚軸之苦 味及源自雌馬酚之苦味，作 味上必須非常小心注意。然材加以利用時，在風 種從來未健導到的_食m t ^〇〇素材，而不知何種方法讦有 ;ΤΓ:別是，將前述大豆胚_利用在烘 =:力有因供予燒烤步驟叫發生風味劣化， 味乃是一種非常困難之技術課題。 見出Γ 本發明之目的在於提供—種從含雌絲之大立 轴發酵物萃取包含雌-之有用成分的萃取物以及製造 9 200906315 其之方法。此外，本發 有物有效率地純化出高-含 之目的在於提供—種 雌㈣之方法。再者，本發明 <叩素材，其含有以雌馬酚產生微生 物使大旦胚軸發酵而 5 10 15 萃取物，料味已受到改/雌馬⑽絲轴發酵物或其 供-種食品(特別㈣:善:另外，本發明之目的在於提 發酵物，且呈現出良好之^含有該含雌馬敎豆胚軸 提供含有含雌馬 味。此外，本發明之目的在於， 的食品。…錄㈣酵物或其萃取物之各種形態 解決課題之手段 .Ά θ人為了解決上述課題而精心研討，意外發 6 n錢作為萃取溶綱對含雌‘㈣之大豆胚 二發酵物進行萃取處理，可有效率地從該大立胚轴發酵物 卒取出包含雖馬盼之有用成分。再者，亦發現對含雌馬酚 之大豆胚軸發酵物依序實施使用乙醇水溶液之萃取處理及 使用乙醇之萃取處理，可藉此有效率地萃取出雌馬盼及黃 五素黃酮’同時可減低造成溫味、苦味十未等不快氣味 之成因的皂苷含量。 再者，本案發明人發現，將含雌馬酚之大豆胚軸發 斗为旱年 、净於pH2〜6之水溶液中並回收不溶物，再使用乙醇水溶 ’夜作為萃取溶劑對如此製得之不溶物進行萃取處理，可藉 此製出雌馬酚係經高濃度濃縮之含雌馬酚萃取物。 此外，本案發明人發現，藉由歷經以下（1)〜(5)之操作， 可有效率且簡便地純化出高純度雌馬酚，即：（1)使用乙醇 20 200906315 水ί夜萃取處理雌馬盼含有物；⑺再使用乙醇萃取處理所 知萃取物，（3)使用己燒萃取處理所得萃取物；⑷使用己炫 及鱗之混合液萃取處理不溶性分液；（5)將所得萃取液供予 二氧化矽凝膠管柱層析，獲得含雌馬酚之分液。 5 ⑽，本案發曰月人發現，藉由歷經下述(i)〜(iii)之操作 亦可有效率且簡便地純化出高純度雌馬紛：⑴使用特定有 劑或其含水有機溶劑萃取處理雌馬紛含有物，濃縮所 仔萃取液’⑻將所得濃縮物供予二氧化石夕凝膠管柱層析， 獲得含雌馬紛之分液；⑽從所得含雌馬盼之 10劑，對所得殘留物進行再結晶化處理。 、办 本案發明人發現 .-右彳史3雌馬酚之大豆胚軸發 秘物或其萃取物分散於可可塊而成食品素材，源自含雌馬 -之大絲軸發酵物的苦味將會受到抑制，而呈現出良好 15 20 。此外，亦發現此種食品素材可無損風味地利用在 /、烤點心為始之各種食品上。 _ 纟案發明人成功地開發出配合有含雄馬紛之大 丑胚轴發酵物或其萃取物的各種形態食品。 本發明係基於上述見解而更反覆改良而終至完成者。 本毛明&供下述含雌馬盼之萃取物的製造方法。 第w項—種含雌馬紛之萃取物之製造方法，包含： 之大1步驟’係使用乙醇水溶液作為萃取溶劑，將含雄馬紛 ，胚軸發酵物進行萃取處理並回收萃取液者。 第1-2項如第Μ項之製造方法，其更台人黛 驟’即：你笙Τίμ 如 3弟1-2步 弟Μ步驟所得卒取液去除萃取溶劑後使用乙 11 200906315 醇作為萃取溶劑進行萃取處理，並回收萃取液者。 第1-3項如第Μ項之製造方法，其中該第μ步驟所 使用之乙醇水溶液中的乙醇濃度為2〇〜98容量％。 第1-4項如第1-1至1-3項中任一項之製造方法，其中 5忒含雌馬酚之大豆胚軸發酵物係以雌馬酚產生微生物使大 豆胚軸發酵而獲得者。 第1-5項一種含雌馬酚之萃取物的製造方法，係從含 雌馬酚之大豆胚軸發酵物製造含雌馬酚之萃取物，包含下 述第11_1步驟及第ΙΙ-2步驟，即：第叫步驟，係使含雌馬紛 10之大丑胚軸發酵物懸浮於水中，加入酸調整為ρΗ2〜6,並回 收不溶物，及，第11_2步驟，使用乙醇水溶液作為萃取溶劑， 對該第11_1步‘_得不溶物進行萃取處理，並回收萃取液。 第1-6項如第丨_5項之製造方法，其中該第π_〗步驟所 使用之酸為乙酸。 15 此外’本發明提供如下所揭示之含雌馬㈣取物。 第2-1項一種含雌馬酚之萃取物，係使用乙醇水溶液 作為萃取溶劑，將含雌馬盼之大立胚轴發酵物進行萃取處 理而獲得者。 第2-2項-種含雌馬盼之萃取物，係對含雌馬紛之大 2〇豆胚轴發酵物依序實施使用乙醇水溶液之萃取處理及使用 乙醇之萃取處理而製得者。 第2-3項-種含雌馬紛之萃取物，係以如第Μ項至第 1-6項中任一項之製造方法製得者。 此外’本發明提供如下揭示之雌馬_屯化方法。 12 200906315 、第3-1項一種從雌馬紛含有物純化㈣㈣u 法，包含以下第M步驟〜第】_5步驟，即：第Μ步驟，使用 乙醇水溶液作為萃取溶劑，將雌馬时有物進行萃取處 理，並回收萃取液；第步驟，從該第W步驟所得萃取液 5絲溶舰，使用乙醇作為萃取溶劑，對所得殘留物進行 f取處理，並回收萃取液；第1-3步驟，從該第m步驟所得 萃取液去除溶劑後，使所得殘留物懸浮於己院中並回收 不溶物；第M步驟，使用己烧與驗之混合液作為萃取溶 劑，對該第1-3步驟所得不溶物進行萃取處理，並回收萃取 10液；及第1-5步驟，將前述第1_4步驟所得萃取液供予二氧化 矽凝膠管柱層析，並回收含有雌馬酚之分液。 第3-2項如第3-1項之純化方法，其中該雌馬酚含有物 為含有雌馬朌之發酵物。 第3-3項如第3-2項之純化方法，其中該含有雌馬酚之 15發酵物係以雌馬酚產生微生物使大豆胚軸發酵而獲得的含 雌馬酴之大豆胚軸發酵物。 第3-4項如第3-1至3-3項中任一項之純化方法，其中 該第1-4步驟所使用之萃取溶劑為己烷與二乙醚之混合液。 第3-5項如第3-1至3-4中任一項之純化方法，其中該 2〇第I·4步驟所使用之萃取溶劑係己烷與醚為1〇 : 9〇〜2〇 : 8〇 之容量比的混合液。 第3-6項如第3-1至3-5中任一項之製造方法，其中該 第1-1步驟所用乙醇水溶液中之乙醇濃度為2〇〜98容量0/〇。 第3-7項一種從雌馬紛含有物純化出雌馬齡之方 13 200906315 法，包含下述第2-1步驟〜第2_3步驟，即：第2_丨步驟，係使 用選自+於由乙酸乙S旨、醇、丙酮、二魏、乙猜、二乙越 =甲苯所構成群組中之至少i種有機溶劑或是其含水有機 溶劑作為萃取輔’料㈣含有物進行萃取處理，並回 收萃取液者；第2·2步驟’係將該第2-1步驟所得萃取液供予 :乳化碎凝膠管柱層析，而獲得含有雌馬吩之分液者；及， 第2-3步驟，係從該第2_2步驟所得含雖馬盼之分液去除容 劑’對所得殘留物進行再結晶化處理，並回收析出之結: 者。 ιυ 15 20 第W項如第3·7項之純化方法，其於第23步驟中， 係：使用乙酸乙酯與己烷之混合液進行再結晶處理後 進行使用醇水溶液之再結晶處理。 明。本發明更提供下揭有關食品素材及利用其之食品的發 第4 1項-種食品素材，係使含有_馬 發酵物或其萃取物分散於可可塊而成者。 胚軸 =2項 > 第4]項之食品素材，其中相對於該含有雄 ㈣之大絲轴發酵物或其萃取物刚重量份，所含可可塊 係佔10〜20〇〇重量份之比率。 第4-3項如第4]或4·2項之食 生物Γ4具項右如第4-3項之食品素材，其中該雄馬紛產生微 物係一具有貧化木質素黃_而產生雌馬紛之能力的微 14 200906315 生物，且該木質素黃酮類係選自木質素黃酮配糖體、木質 素黃酮及二氫木質素黃酮所構成群組中之至少1種。 第4-5項如第4-1至4-4項中任一項之食品素材，其係 呈粒狀、片狀、或平板狀。 5 第4-6項一種含雌馬酚之食品，含有如第4-1至4-5項 中任一項之食品素材。 第4-7項如第4-6項之含雌馬酚之食品，其係烘烤點 心。 本發明更提供下揭食品。 10 第5-1項一種食品，含有含雖馬紛之大豆胚軸發酵物 或其萃取物1 第5-2項 或其萃取物1 第5-3項 種飲料，含有含雌馬酚之大豆胚軸發酵物 一種補充品（supp丨ement)，含有含雌馬酌·之大 15 豆胚軸發酵物或其萃取物。 第5-4項一種奶油狀食品，含有含雌馬酚之大豆胚軸 發酵物或其萃取物。 第5-5項一種甜點，含有含雌馬酚之大豆胚軸發酵物 或其萃取物。 種點心，含有含雌馬酚之大豆胚軸發酵物 20 第5-6項 或其萃取物。 第5-7項一種調味料，含有含雌馬紛之大豆胚軸發酵 物或其萃取物。 第5-8項一種殺菌袋食品，含有含雌馬酚之大豆胚軸 15 200906315 發酵物或其萃取物。 第5-9項一種加工肉食品，含有含雌馬酚之大豆胚軸 發酵物或其萃取物。 第5-10項一種膏狀食品，含有含雌馬酚之大豆胚軸 5 發酵物或其萃取物。 第5-11項一種蛋加工食品，含有含雌馬酚之大豆胚 軸發酵物或其萃取物。 第5-12項一種罐頭或罐裝食品，含有含雌馬酚之大 豆胚軸發酵物或其萃取物。 10 第5-13項一種麵包，含有含雌馬酚之大豆胚轴發酵 物或其萃取物。 第5-14項一種冷;東甜點，含有含雕馬酌·之大丑胚轴 發酵物或其萃取物。 第5-15項一種大豆加工食品，含有含雌馬酚之大豆 15 胚軸發酵物或其萃取物。 第5-16項一種米飯食品，含有含雌馬酚之大豆胚軸 發酵物或其萃取物。 第5-17項一種湯品，含有含雌馬酚之大豆胚軸發酵 物或其萃取物。 20 第5-18項如第5-1至5-18項中任一項之食品，其中該 含雌馬紛之大豆胚軸發酵物係以雌馬紛產生微生物使大豆 胚軸發酵而獲得者。 第5-19項如第5-18項之食品，其中該雌馬酚產生微生 物係一具有將木質素黃酮類資化而產生雌馬酚之能力的微 16 200906315 生物，且該木質素黃酮類係選自於由木質素黃酮配糖體、 木質素黃酮及二氫木質素黃鲷所構成群組中之至少丨種。 圖式簡單說明 第1圖顯示檢測參考例Μ所得含雌馬酚之大豆胚軸發 5酵物、大豆子葉及大豆胚軸所含總蛋白質之結果（電泳圖）。 第2圖顯示檢測參考例Μ所得含雌馬酚之大豆胚軸發 酵物、大豆子葉及大豆胚軸所含主要致敏原（Gym4、 Gm30K、Gm28K)之結果（電泳圖）。 第3圖顯示檢測參考例Μ所得含雌馬酚之大豆胚軸發 10酵物、大豆子葉及大豆胚軸所含主要致敏原（7S球蛋白 mix、油體蛋白（Oleosins)、胰蛋白酶抑制因子（trypsin inhibitor))之結果（電泳圖）。 第4圖顯示實施例1-1中以tLC分析萃取液卜2及2_2之 雌馬齡含量的結果。 15 第5圖顯示實靶例1-1中以TLC分析萃取液ι_2及2-2之 皂苷含量的結果。 第6圖顯示實施例1-1中以TLC分析萃取液u及殘渣2 之雌馬盼含量的結果。 S7圖顯示實施例M中以TLC分析萃取液卜2及殘逢2 20 之皂苷含量的結果。 帛示實施例2] t將藉二氧切凝膠管柱層析溶 出之溶出液供予TLC(薄層層析）分析的結果。 發明之效果 依據本發明之含雌馬酚之萃取物的製造方法，可從含 17 200906315 雌馬盼之大丑胚軸發酵物有效率地萃取出包含雌馬齡之有 用成分，進而製造出作為機能性食品素材甚有用的含雌馬 =之萃取物。此外，11由對含雌糾之大⑽軸發酵物依 實施使用乙私水溶液之萃取處理及使用乙醇之萃取广 理，可獲得含高濃度雌㈣及黃豆素料且料(人人不： 氣=之成因）減低的㈣馬敎萃取物。料，絲雕馬盼 =取物具有不致對風味造成不良影響而可配合於食品中 此外，依據本發明之純化方法，可簡便且有效率地從 雌馬紛含有物獲得高純度雌馬ι特別是，即使雌馬齡含 有物中混有與雌騎結_似之異黃_，本㈣之純化方 法可除切等異黃㈣高純度地純化_騎。因此，本 發明之純化方法適合❹在從含有多量異黃社含雖馬盼 的發酵物中純化出雌馬酴。 再者’使含雌馬紛之大豆胚轴發酵物或其萃取物分散 至可可塊而成的本發明食品素材在含有含雌馬狀大豆胚 轴發酵物或其萃取物的同時，苦味仍受到抑制而具有良好 之風味。此外，本發明之食品素材具有可利用於各種食品 :不會伴有風味惡化的優點。另外，即使以粒狀或片狀形 :::知之食品素材配合至烘烤點心中，由於烘烤步驟使 :素材所含成分有擴散至哄烤點心整體的傾向，有時 ^法獲得所需之風味。相料此’本發明之食品素材係藉 %可可塊使含雌馬狀大豆_發酵物處於被包住之狀 •I因此，即使以粒狀或片狀利用在供烤點心上，亦可抑 18 200906315 10 制食σα素材所含有效成分之擴散，可呈現所期望之八味 再者，依照本發明之各種形態的食品，可享受美於含 雌馬酚之大豆胚軸發酵物或其萃取物的有用生理作^ 3 另外，含雌馬酚之大豆胚軸發酵物或其萃取物中=軸所含致敏原減低，本發明之食品素材及:種: 也之食m亦具有大㈣敏症人士亦可安全攝取之優點。 【實施方式】 本發明之最佳實施型態 茲就本發明說明如下。 1·金有雌愚废之萃取物的製造方沐 :::==r_造方法大致分為二： 法及第11方法等2種手法。兹就本發明製 15 造方法中用作原料 20 的含有雌馬齡之大豆胚轴發酵物以及第！ 具體内容詳述於下。 本發明之含有雌騎之萃取物的 雌馬齡之大豆胚_酵物 L使用含有 大豆胚軸發酵物加以說明。…’以下就含有雌馬盼之 含有雌馬酚之大豆 生物使大豆胚軸發_^==雌馬盼產生微 使用於製造該含有雄馬盼之大豆胚轴^ 產生微生物係使用具有資$酵物的雌馬酚 、化木貝素黃鲖類而產生雌馬酚之 方法及第II方法 之 19 200906315 能力（代謝活性）的微生物，且該木質素黃酮類係選自木質素 黃酮配糖體、木質素黃酮及二氫木質素黃綱所構成群組中 至少1種。於此，具體來說，木質素黃酮配糖體可列舉如大 豆苷（Daidzin)、丙二醯大豆苷（Malonyl Daidzin)及乙酿大豆 5苷等。 前述雌馬酚產生微生物僅需為食品衛生上可接受且具 有上述能力者即可，未特別受限，可使用習用公知或以通 常方法篩選者。例如，已知於格氏乳球菌（Zflci〇c〇cc⑽ garWe似）等隸屬於乳球菌屬之微生物；中間鏈球菌 10 、星狀鏈球菌（加印价此⑶玉 ⑶《你//α如）等隸屬於鏈球菌屬之微生物；印形類桿菌 (Racier⑴ον加⑽）等隸屬於類桿菌屬之微生物之中存有 具雌馬酚產生能之微生物。雌馬酚產生微生物之中，較佳 者可列舉如乳球菌屬及鏈球菌屬等之乳酸菌，更佳者可列 15舉如隸屬於乳球菌屬之乳酸菌，而尤佳者可列舉如格氏乳 球菌。舉例來說，可以有無雌馬酚產生能作為指標而從人 類糞便中離析出雌馬紛產生微生物。至於前述雌馬紛產生 微生物，本案發明人等業已寄存有從人類糞便離析並經鑑 定之菌株，即，乳球菌20_92(FERM Βρ·1〇〇36號）、鏈球菌 2〇 E-23-17(FERM ΒΡ-6436號）、鏈球菌 A6g225(ferm Βρ 6437 號)及類桿菌E-23-15(FERM ΒΡ_6435號），本發明可使用該 等寄存菌株。該等寄存菌株中，乳球菌2〇_92可適於使用。 該含雌馬酚之大豆胚軸發酵物可使用大豆胚轴作為發 酵原料而製出。大豆胚軸係指大豆發芽時會成為幼芽、幼 20 200906315 根之部分，e知含❹量木質素相轉體及木 等木質素黃_貞。本發賴使狀大豆叫以所含木:鋼 黃酮類未明顯損失為限，大豆產地及加工 貝素 <男無並未特別 10 15 受到限制。例如，可為生鮮狀態者；從業已供加熱處理 乾燥處理、蒸錢理等之大豆分離出者；以及將從未加^ 大豆分離出之絲供加減理、乾燥處理或蒸煮處理t 中之任*白可。此外，使用之大立胚袖亦可為業已 脂處理及脫蛋白處理者。另，所使用之大⑽軸、、 =特別受限，可為粉末狀、經粉碎或破碎之㈣或塊狀並 從更有效率地產生雌馬酚之觀點來看，宜使用粉末 豆胚軸。 71 之大 種 將適量之水加入大豆胚軸調整水分含量於其中 述雌馬盼產生微生物以進行大豆胚轴之發酵處理。 添加於大豆胚軸之水量可依所用雌馬酚產生 糊及發酵槽之種類等而適宜地狀。通常僅需於發酵^ 始日禮大絲轴與水以下述比例共存即可：相對於大豆肢 軸(乾燥重置換算）1〇〇重量份，水為4⑼〜4〇㈨重量份且宜 為500〜2000重量份，更宜為800〜1000重量份。 且 此外，大豆胚軸發酵時，以促進發酵效率及提高發酵 20物之風味等作為目的，成為發酵原料之大豆胚軸令可依需 要而添加酵母萃取、聚蛋白練、肉精等氮源 ;葡萄糖、蔗 糖等碳源；磷酸鹽、碳酸鹽、硫酸鹽等無機鹽；維生素類；' 胺基酸等營養成分。特別是使用具有將精胺酸轉換為鳥胺 酸之能力者（以下，記為「鳥胺酸•雌馬盼產生微生物」） 21 200906315 作為雌馬酴產生微生物時，可於大豆胜财添加精胺 行發酵’可藉此使所得發酵物含有烏胺酸。此時， 說，精胺酸之添加量可例示如：相對於大豆胚袖(乾， 換算_重量份，精胺酸為〇.5〜3重量份程度。此外，、董 雌馬紛產生能與從精_轉換為烏贿之轉換能二 以習知之篩選方法獲得烏胺酸•雌騎產生微生物。舉t 2言，鳥胺酸•雌騎產生微生物可選自格氏乳龙 具體例可列舉如乳球g2G娜職购_號）。、 10 15 20 ▲再者，就所使用發酵原料（大豆胚轴含有物 二僅需可使雌馬粉產生微生物生育即可，並未特別受限而 酵^可使雌祕產生微生物良好增殖的觀點來看 酵原料之PH贱㈣至8〜7程度，增為83〜8^發 加〜!ϋ、’所使狀料射'軸含有物）更可事先六 3刖述木質素黃_貞之異黃酮 、+ 3里更為^ ’而進—步提升其有用性。 育特Γ胚轴之發酵可於因應所用雕馬齡產生微生物之生 I的環境條件下實施說， 體列舉之雌馬酚產U “…一 資疋使用刚述具 性條件下進行。’，絲*之發酵即係於厭氧 條件即二度僅需為適合雌馬酚產生微生物生育之Blacklock N, Matos-Ferreira A, Abranches-Monteiro L, Correia R, Lloyd S, Griffiths K. Lignans and isoflavonoids in plasma and prostatic fluid in men : Samples from Portugal, Hong Kong, and the United Kingdom. Prostate 32:122- 128, 200906315 1997 [Non-Patent Document 4] Tammy EH, Paul DM, Paul GF, Robert D, Stephen B, Kenneth J, Ray M, Lorraine GO, Kristiina W, Holly MS, Karen JG. Long-term dietary habits affect soy 5 isoflavone metabolism and accumulation in prostatic fluid in Caucasian men. J Nutr 135:1400-1406, 2005 t day and month] Disclosure of the invention The problem to be solved by the invention 10 So far, the inventors of the present invention have found that to produce equol The large homeosymmetric fermentation obtained by the microorganism to ferment the large ugly hypocotyl can be used as a food material containing the eagle horse. The soybean hypocotyl fermented product not only has the desirable ingredients of soybean, but also has isoflavones and soap (4) derived from soybeans, according to which it can exhibit 15 W as a functional material, even M@ depleted yeast derived from soybeans. Hypoallergenic allergens are 'useful as a hypoallergenic element. As described above, the aforementioned soybean hypocotyls which are present in the present month have useful physiological activities, and it is useful for the low-induced yeast to be based on the ingredients containing the ingredients. Therefore, it is known that it is a functional food. On the other hand, the type of soybean hypocotyls used for the production of the soybean hypocotyl acid plate 6 and the amount of the females expected to produce the females are different, and are mostly about 1% by weight. In this case, if the material is improved by the microbial species of Mapan, it is possible to respond to a variety of types of female horse-containing foods. ^Diversification 4, Rong (4) Provided So far, the aforementioned soybean embryo 8 200906315 shaft ferment itself is not conventionally known, and the soybean hypocotyl ferment extract is extracted from the female (IV) ^ effectively from the aforementioned unclear. 3 using the ingredients, this point is still better, by the above-mentioned soybean Shengtuo fermented product compared to the chemical synthesis method and the rich (four) female horses look forward to "the advantage. However, by the fermentation method, ... The metabolites are also contained. (4) The inclusions also include, depending on the type of raw materials used in the fermentation: there are various components of::::. The substance may also contain gamma powder containing 10 15 20 Therefore, if desired The equol and the additive used in the sigma sigma are used in a technique of equestrian beauty, and the purity of the purified female _ is lacking. However, until now, there is a technique that is not reported. Therefore, it is necessary Established = in purification _ effect and simple pure (four) horse _ high purity technology. Wang industry application, 7 has a bite (1) the large silk shaft ferment and has a bitter taste derived from the giant stalk hypocotyl and derived from female The bitter taste of the p-phenol, must be very careful in the taste. When the material is used, the _ mt 〇〇 〇〇 material that has never been guided by the wind, I do not know what method is available; ΤΓ: Do not, will The aforementioned soybean embryo_utilization in the baking =: force has a cause of grilling Flavor is a very difficult technical problem. The object of the present invention is to provide an extract for extracting a useful component containing an estradiol from a large vertical shaft ferment containing a female silk and to produce the method of 200906315. The present invention has an effect of efficiently purifying a high-inclusive method for providing a female (four) method. Further, the present invention relates to a raw material containing an equol-producing microorganism for fermenting a large dendritic hypocotyl 5 10 15 extract, the taste has been changed / female horse (10) silk shaft ferment or its food - (special (four): good: In addition, the purpose of the present invention is to extract the ferment, and present a good ^ containing the female The horse caries hypocotyls are provided with a female-containing scent. The purpose of the present invention is to provide a food for the purpose of solving various problems in the form of a solution to the above-mentioned problems. Unexpectedly, 6 n of money was used as an extraction solvate to extract the fermented soybean (E) embryo containing the female '(4), and the fermented material of the large hypocotyl can be efficiently removed from the fermented material containing the desired ingredients. Find The equol-containing soybean hypocotyl fermented material is sequentially subjected to extraction treatment using an aqueous ethanol solution and extraction treatment using ethanol, thereby efficiently extracting the equand and the flavonoids of the phos- saponin, and simultaneously reducing the temperature and bitterness. Furthermore, the inventors of the present invention found that the equol-containing soybean hypocotyl is a dry year, is purified from an aqueous solution having a pH of 2 to 6 and recovers insoluble matter, and then is dissolved in ethanol. 'Night as an extraction solvent, the insoluble matter thus obtained is subjected to extraction treatment, whereby an equol-containing equol-containing extract containing equol can be produced. Further, the inventors of the present invention found that by the following (1) ) (5) operation, can efficiently and easily purify high-purity equol, namely: (1) using ethanol 20 200906315 water gluten extraction treatment of equestrian products; (7) using ethanol extraction treatment Extracting, (3) extracting the obtained extract by using hexane, and (4) extracting the insoluble liquid by using a mixture of hexazone and scale; (5) supplying the obtained extract to cerium oxide gel column chromatography, Containing liquid separation to obtain the equol. 5 (10), in this case, the person found that the high-purity equine can be efficiently and simply purified by the following operations (i) to (iii): (1) using a specific agent or its aqueous organic solvent Extracting and treating the equine horses, concentrating the extracts of the plants '(8), supplying the obtained concentrates to the silica gel column chromatography to obtain a liquid containing the female horses; (10) 10 from the obtained female horses The resulting residue was subjected to recrystallization treatment. The inventor of the case found that - the right scorpion history 3 equol soybean hypocotyl primate or its extract is dispersed in the cocoa mass into a food material, derived from the bitter taste of the equine-containing fermented shaft Will be suppressed and present a good 15 20 . In addition, it has been found that such food materials can be used in various foods starting from /, roasted snacks without loss of flavor. _ The inventor succeeded in developing various morphological foods that were combined with a large ugly hypocotyl fermented product or an extract thereof. The present invention is based on the above findings and is further improved to the end. Ben Maoming & The method for producing the following extract containing the espresso. The wth item - a method for producing an extract containing a female horse, comprising: the first step of using an aqueous solution of ethanol as an extraction solvent, extracting the male horse and the hypocotyl ferment, and recovering the extract. Item 1-2, such as the manufacturing method of the third item, is more step-by-step: that is, you 笙Τίμ, such as the 3 brothers 1-2 steps, the stepping solution obtained by removing the extraction solvent, using B 11 200906315 alcohol as extraction The solvent is subjected to extraction treatment and the extract is recovered. Item 1-3, wherein the ethanol concentration in the aqueous ethanol solution used in the step (μ) is from 2 to 98% by volume. The method of any one of items 1-1 to 1-3, wherein 5 忒 equol-containing soybean hypocotyl fermentate is obtained by fermenting soybean hypocotyls with equol-producing microorganisms By. Item 1-5. A method for producing an equol-containing extract, which comprises producing an equol-containing extract from an equol-containing soybean hypocotyl ferment, comprising the following steps 11_1 and ΙΙ-2 , that is, the first step is to suspend the ugly hypocotyl fermented product containing the female horses in water, adjust the acid to ρΗ2~6, and recover the insoluble matter, and, in step 11_2, use the aqueous ethanol solution as the extraction solvent. The step 11_1 '_ insoluble matter is subjected to extraction treatment, and the extract is recovered. Item 1-6, wherein the acid used in the step π_〗 is acetic acid. Further, the present invention provides a female-containing (four)-containing material as disclosed below. Item 2-1 An equol-containing extract obtained by extracting a fermented product of the erectus containing the esculentus using an aqueous solution of ethanol as an extraction solvent. Item 2-2 - an extract containing an esculentus, which is obtained by extracting an aqueous solution of an aqueous ethanol and extracting it with ethanol using a fermented product containing a large number of female horses. Item 2-3 - An extract containing a female horse, which is obtained by the production method according to any one of items 1-6 to 1-6. Further, the present invention provides a female horse smashing method as disclosed below. 12 200906315, Item 3-1 A method for purifying (4) (4) u from the equine horses, comprising the following steps M to _5, ie, the third step, using an aqueous solution of ethanol as an extraction solvent, and carrying out the equine horses Extracting and recovering the extract; in the first step, the extract obtained from the step W is a solution of 5 filaments, using ethanol as an extraction solvent, and the obtained residue is subjected to f treatment, and the extract is recovered; steps 1-3, After removing the solvent from the extract obtained in the step m, the obtained residue is suspended in the hospital and the insoluble matter is recovered. In the step M, the mixture of the calcined and the test mixture is used as an extraction solvent, and the first step is obtained. The insoluble matter is subjected to extraction treatment, and the extract 10 liquid is recovered; and in the steps 1-5, the extract obtained in the above step _4 is supplied to the ceria gel column chromatography, and the fraction containing equol is recovered. Item 3-2. The purification method according to item 3-1, wherein the equol-containing substance is a fermented product containing an estrogen. Item 3-3, the purification method according to Item 3-2, wherein the equol-containing 15 ferment is a soybean-bearing hypocotyl fermented product obtained by fermenting soybean hypocotyls with an equol-producing microorganism. . The purification method according to any one of items 3-1 to 3-3, wherein the extraction solvent used in the steps 1-4 is a mixture of hexane and diethyl ether. The purification method according to any one of items 3-1 to 3-4, wherein the extraction solvent used in the step 2/1.4 is hexane and ether is 1〇: 9〇~2〇 : Mixture of 8 容量 capacity ratio. The manufacturing method according to any one of the items 3-1 to 3-5, wherein the ethanol concentration in the aqueous ethanol solution used in the step 1-1 is 2 〇 98 98 98 。. Item 3-7 A method for purifying the female horse age from the equine horses 13 200906315 method, comprising the following steps 2-1 to 2_3, that is, the second step, which is selected from + Extracting at least one kind of organic solvent in the group consisting of acetic acid ethyl acetate, alcohol, acetone, diwei, acetyl, diethylidene or toluene or an aqueous organic solvent thereof as an auxiliary material of the extraction auxiliary (four), And extracting the extract; the step 2·2 is to supply the extract obtained in the step 2-1: emulsified gel column chromatography to obtain a liquid containing the female equator; and, the second In the step -3, the obtained residue is subjected to recrystallization treatment from the liquid separation-removing agent of the mixture obtained in the second step 2, and the precipitate is recovered and precipitated. Ιυ 15 20 The purification method of the item W, item 3, item 7, in the step 23, is carried out by recrystallization treatment using a mixture of ethyl acetate and hexane, followed by recrystallization treatment using an aqueous alcohol solution. Bright. Further, the present invention provides a food material according to the fourth aspect of the food material and the food product using the same, which is obtained by dispersing a zebra ferment or an extract thereof in a cocoa mass. Hypocotyl = 2 item> The food material of item 4, wherein the cocoa mass is 10 to 20 parts by weight with respect to the orthodontic product containing the male (4) or the extract thereof. ratio. Item 4-3 is the food material of item 4] or item 4 of item 4, and the item of material of item 4-3 is right as in item 4-3, wherein the male horse produces a micro-system with a depleted lignin yellow _ The female zebra flavone is at least one selected from the group consisting of lignin flavone glycoside, lignin flavone, and dihydrolignin flavone. Item 4-5. The food material according to any one of items 4-1 to 4-4, which is in the form of a granule, a sheet, or a plate. Item 4-6 An equol-containing food containing the food material of any one of items 4-1 to 4-5. Items 4-7, such as equol-containing foods in items 4-6, are baked hearts. The invention further provides a food product. 10 Item 5-1 A food containing a fermented soybean hypocotyl or its extract 1 No. 5-2 or its extract 1 No. 5-3, containing equol-containing soybeans A hypocotyl fermented product is a supplement (supp丨ement) containing a large 15 pea hypocotyl fermented product containing an equestrian horse or an extract thereof. Item 5-4 A creamy food containing equol-containing soybean hypocotyl ferment or an extract thereof. Item 5-5 A dessert comprising an equol-containing soybean hypocotyl fermentate or an extract thereof. A snack containing equol-containing soybean hypocotyl fermentate 20 Items 5-6 or its extract. Item 5-7 A seasoning containing a soy embryonic fermented product containing an estrogen or an extract thereof. Item 5-8 A sterilized bag food containing equol-containing soybean hypocotyl 15 200906315 Fermentation or an extract thereof. Item 5-9 A processed meat product comprising an equol-containing soybean hypocotyl ferment or an extract thereof. Item 5-10 A creamy food containing equol-containing soybean hypocotyl 5 ferment or an extract thereof. Item 5-11 An egg processed food comprising an equol-containing soybean blast spindle ferment or an extract thereof. Item 5-12 A canned or canned food containing equol-derived soybean hypocotyl fermentate or an extract thereof. 10 Item 5-13 A bread containing equol-containing soybean hypocotyl fermentate or an extract thereof. Items 5-14 are a cold; East dessert containing a large ugly hypocotyl ferment or its extract. Item 5-15 A soybean processed food comprising equol-containing soybean 15 hypocotyl fermentate or an extract thereof. Item 5-16 A rice food containing equol-containing soybean hypocotyl ferment or an extract thereof. Item 5-17 A soup containing equol-containing soybean hypocotyl fermentate or an extract thereof. The food of any one of items 5-1 to 5-18, wherein the soy hybrid hypocotyl fermented product of the female horse is obtained by fermenting soybean hypocotyls by a female horse . Item No. 5-19, wherein the equol-producing microorganism is a micro 16 200906315 organism having the ability to produce equol by lignin flavonoids, and the lignin flavonoids It is selected from the group consisting of lignin flavone glycoside, lignin flavone, and dihydrolignin xanthine. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 shows the results of electrophoresis (electrophoresis) of the total protein contained in the equol-containing soybean hypocotyls, soybean cotyledon and soybean hypocotyls. Fig. 2 shows the results (electropherogram) of the main allergens (Gym4, Gm30K, Gm28K) contained in the equol-containing soybean hypocotyls, soybean cotyledons and soybean hypocotyls obtained in the reference example. Figure 3 shows the main allergens (7S globulin mix, oily protein (Oleosins), trypsin inhibitor) contained in the equol-containing soybean hypocotyls 10, soy cotyledon and soybean hypocotyls obtained from the reference example. (trypsin inhibitor)) (electropherogram). Fig. 4 shows the results of analyzing the estrogen content of the extracts 2 and 2_2 by tLC in Example 1-1. 15 Fig. 5 shows the results of analyzing the saponin contents of the extracts ι_2 and 2-2 by TLC in the actual target 1-1. Fig. 6 shows the results of analyzing the etoposide content of the extract u and the residue 2 by TLC in Example 1-1. The S7 chart shows the results of analyzing the saponin content of the extract 2 and the residue 2 20 by TLC in Example M. Illustrative Example 2] t The result of TLC (thin layer chromatography) analysis was carried out by extracting the eluate eluted by the dioxo gel column chromatography. Advantageous Effects of Invention According to the method for producing an equol-containing extract of the present invention, a useful component containing an estrogen age can be efficiently extracted from a large ugly hypocotyl fermented product containing 17 200906315 Functional food material is very useful for extracts containing female horses. In addition, 11 is prepared by extracting the large (10) axis fermented product containing the females and using the extraction process of the aqueous solution of Ethyl alcohol and using the extraction of ethanol, and obtaining the high concentration of the female (tetra) and soy material and the material (everyone is not: gas) = the cause of the decrease) (four) horse scorpion extract. Material, silk carving Mawang = extract has no adverse effect on the flavor and can be blended into the food. In addition, according to the purification method of the present invention, the high-purity female horse can be easily and efficiently obtained from the equine horses. Yes, even if the female age contains a mixture of the same as the female rider, the purification method of the present (4) can be purified in addition to the cut yellow (4) high purity. Therefore, the purification method of the present invention is suitable for purifying estrogen from a fermentation product containing a large amount of isoamylose. Furthermore, the food material of the present invention obtained by dispersing a soybean-containing soybean hypocotyl fermented product or an extract thereof into a cocoa mass contains a fermented horse-like soybean hypocotyl fermentate or an extract thereof, and the bitterness is still received. Suppressed with a good flavor. Further, the food material of the present invention has an advantage that it can be utilized for various foods without accompanying deterioration of flavor. In addition, even if the food material of the grain or the sheet shape is blended into the baked snack, the baking step causes the ingredients contained in the material to have a tendency to spread to the whole of the baked snack, and sometimes the desired method is obtained. The flavor. It is said that the food material of the present invention uses the cocoa mass to make the female-like soybean-fermented product in the form of being wrapped. Therefore, even if it is used in the form of granules or flakes, it can be suppressed. 18 200906315 10 The spread of the active ingredients contained in the σα material can be expressed as the desired eight flavors. In addition, the equol-containing soybean hypocotyl fermented product or its extraction can be enjoyed according to the various forms of the food of the present invention. Useful physiological work of the product ^ 3 In addition, the equol-containing soybean hypocotyl fermented product or its extract = the allergen contained in the shaft is reduced, the food material of the present invention and the species: the food m also has a large (four) People with sensitive conditions can also take advantage of safe intake. [Embodiment] BEST MODE FOR CARRYING OUT THE INVENTION The present invention will now be described. 1. The manufacture of extracts of gold and females is: ::==== The method of making is roughly divided into two methods: the method and the eleventh method. The soy embryonic fermented product of the equestrian age of the horse used as the raw material 20 in the method of the present invention is as follows! The details are detailed below. The estrogen-derived soybean embryo-protein L containing the extract of the female rider of the present invention is described using a soybean-containing hypocotyl fermentate. ...'The following contains the equol-containing soybean organisms that make the soybean hypocotyls _^==Essence of the horses to produce micro-systems for the production of the soybean embryos containing the male horses. a method for producing equol by a yeast, a method for producing equol and a method for the production of equol, and a microorganism of the second method, wherein the lignin flavonoid is selected from the group consisting of lignin flavonoids At least one of the group consisting of lignin flavone and dihydrolignin. Here, specifically, the lignin flavonoid glycoside may, for example, be Daidzin, Malonyl Daidzin, and B. The aforementioned equol-producing microorganisms need only be food-healthy and have the above-described ability, and are not particularly limited, and can be used by a conventionally known or conventional method. For example, it is known to be a microorganism belonging to the genus Lactococcus such as Zfc〇c〇cc(10) garWe; Streptococcus mutans 10, Streptococcus mutans (additional price (3) jade (3) "You / / α A microorganism belonging to the genus Streptococcus; a microorganism belonging to the genus Bacteroides such as the genus Bacteroides (Racier (1) ον plus (10)), which contains equol-producing microorganisms. Among the equol-producing microorganisms, preferred are lactic acid bacteria such as Lactococcus and Streptococcus, and more preferably, such as lactic acid bacteria belonging to the genus Lactococcus, and particularly preferred are Lactococcus. For example, the presence or absence of equol can be used as an indicator to isolate females from human feces to produce microorganisms. As for the aforementioned female horses, the inventors have deposited strains isolated and identified from human feces, namely, Lactococcus 20_92 (FERM Βρ·1〇〇36), Streptococcus 2〇E-23-17 (FERM ΒΡ-6436), Streptococcus A6g225 (ferm Βρ 6437), and Bacteroides E-23-15 (FERM ΒΡ_6435), the deposited strains can be used in the present invention. Among these deposited strains, Lactococcus 2〇_92 can be suitably used. The equol-containing soybean hypocotyl fermentate can be produced using soybean hypocotyls as a fermentation material. Soybean hypocotyl refers to the part of the root of the young shoots, young 20 200906315 when the soybeans germinate, and the lignin yellow and lignin such as wood. The hairy soybeans are called to contain wood: steel flavonoids have no obvious loss, soybean origin and processing Besin < males are not particularly 10 15 restricted. For example, it may be a fresh state; a soybean isolate that has been subjected to heat treatment, dry treatment, steaming, etc.; and a yarn that has been separated from the soybeans for addition, reduction, drying, or retort treatment. *White can. In addition, the large cuff sleeves used can also be processed by fat and deproteinized. In addition, the large (10) axis, = used is particularly limited, and may be powdery, pulverized or broken (four) or blocky, and from the viewpoint of producing equol more efficiently, it is preferred to use a powdered bean hypocotyl . Large variety of 71 A proper amount of water is added to the soybean hypocotyl to adjust the moisture content in which the females are expected to produce microorganisms for the fermentation treatment of soybean hypocotyls. The amount of water added to the soybean hypocotyl can be suitably selected depending on the type of equol to be used and the type of the fermentation tank. Usually, it is only necessary to coexist with the water in the following ratio: the weight of the soybean limb (drying conversion conversion) is 1 part by weight, and the water is 4 (9) to 4 〇 (9) parts by weight and preferably It is preferably 500 to 2000 parts by weight, more preferably 800 to 1000 parts by weight. In addition, in the soybean hypocotyl fermentation, the soybean embryo shaft which is a fermentation raw material for the purpose of promoting the fermentation efficiency and the flavor of the fermentation 20, and the like, may add a nitrogen source such as yeast extract, polyprotein or meat, as needed; Carbon sources such as glucose and sucrose; inorganic salts such as phosphates, carbonates, and sulfates; vitamins; and nutrients such as amino acids. In particular, when the ability to convert arginine to ornithine is used (hereinafter, it is referred to as "ornithine and female horse-producing microorganisms") 21 200906315 When the microorganism is produced as a female horse, it can be added to the soybean The amine is fermented' so that the resulting ferment contains uric acid. In this case, the amount of arginine added can be exemplified as follows: relative to the soybean embryo sleeve (dry, converted to 重量 by weight, arginine is 〇. 5 to 3 parts by weight. The conversion from the fine _ to the ebony can be obtained by a conventional screening method to obtain ursolic acid • female riding to produce microorganisms. For example, the avian acid • female riding microorganism can be selected from the specific example of the genus Listed as the milk ball g2G Na job _ number). 10 15 20 ▲ In addition, the fermented raw material used (the soybean hypocotyl containing substance 2 only needs to make the female horse powder produce microorganisms, and is not particularly limited, and the yeast can make the microorganisms proliferate well. From the point of view, the pH of the yeast raw material (4) to 8 to 7 degree, increased to 83~8^ plus ~! ϋ, 'the shape of the material shot 'axis containing matter) can be arbitrarily described in advance 6 3 lignin yellow _ 贞Isoflavones, + 3 in the more ^ 'and advance step by step to enhance its usefulness. Fermentation of the hypocotyls of the genus can be carried out under the environmental conditions of the microorganisms produced by the sculpt of the horses, and the equols produced by the genus are "produced under the conditions of the genus." The fermentation of silk* is based on anaerobic conditions, that is, the second degree only needs to be suitable for the growth of equol-producing microorganisms.

更宜為38〜耽可列舉如2〇〜机，宜為H 發酵時間可依雌馬紛產生量、木質素黃_類殘存量、 22 200906315 雌馬齡產核生物之㈣當 曰’且宜為2〜7曰，更宜為3〜5曰。 ~通常為1〜1〇 3酚產4 ^條件鱗處理㈣狀大該軸發酵物 馬齡產生並蓄積，可表現雄馬紛 發酵物已有雖 胚軸發酵物中之雌馬酚含丄 作用。就大豆 生物及發酵條件等而異，：：二係依所用雌馬齡產生微 平均乾燥重量計(令大 :而5 ’以大五肢輪發酵物之 有雕馬紛為_mg，且^21轉之乾燥重量為㈣），含 該含有雌馬齡之大 邮，更宜為5，。 10 15 20 外，亦含有:大豆*、丙二醯大一：：： 素黃酮及4木質素黃㈣之木朗峨⑽ ^ 成分記為「木質素黃_」）；染料柄(genistm) 1 染料木普、乙酿染料木芽、金雀素黃明、料 等之金雀素黃_類(以下將該等成分記為「金雀= 類」)Lycitin)、丙二醯黃豆*、乙醯 豆素黃嗣、二氣黃豆素黃綱等之黃豆素黃_(以下將該等 成分記為「η素黃_」）等之各種異黃酮，且亦可表現 出該等異黃嗣之有用生理活性。含有雌馬紛之大豆胚軸發 酵物中之異黃酮（包含雌馬盼)含量係依所使用之雌馬料 生微生物及發酵條件等而異，但一般而言，可例示如：含 雌馬紛之大錢轴發酵物的乾燥重量料，異黃嗣為 5〜20mg，且宜為5〜I5mg，更宜為8〜l5mg程度。 此外，含有雌馬紛之大豆胚轴發酵物即使在雖馬盼以 外之異黃喊成之點上，祕有與大豆胚_同之紅成。 23 200906315 特別是’含雌馬酚之大豆胚軸發酵物中之金雀素黃酮類的 總含有率以含雌馬酚之大豆胚軸發酵物的異黃酮總量計， 低至15重量。/Q以下，且宜為12重量％以下；從異黃酮組成之 觀點來看’與發酵前之大豆胚軸相較亦較有利。另，金雀 5素黃嗣類是一種擔憂會作為内分泌擾亂物質而作用之異黃 酮，於食品素材中之濃度以儘量減少為宜(Young HJ，Jodi F, Kimberly FA, Daniel RD, William GH. Effects of dietary daidzein and its metabolite, equol, at physiological concentrations on the growth of estrogen-dependent human 10 breast cancer(MCF-7) tumors implanted in ovariectomized athymic mice. Carcinogenesis 127(4):856-863, 2006參照）。 具體來說，含雌馬酚之大豆胚軸發酵物可例示如以下 之異黃酮組成者（以下之單位「mg」表示大豆胚轴發酵物每 Ig(乾燥重量）之各異黃酮總量）： 15 雌馬酴：1〜20mg，宜為2〜I2mg 木質素黃酮類：0.1〜3〇mg，宜為〇1〜l 5mg 金雀素黃明類：0.05〜2.5mg，宜為〇 〇5〜2吨 黃豆素黃酮類：〇_1〜4mg，宜為2〜3 5mg 此外，含雌馬酚之大豆胚軸 1轉物所含各異黃酮之組 成比率係例示如以下所示範圍（以下之單位「重量％表_ =於含雌馬盼之大豆胚轴發酵物所含全部二二; 里的比例）： 且宜40〜70重量％，更宜為45〜70 雌馬酚：30〜75重量％， 重量。/〇 ; 24 200906315 木質素黃_類：1〜2〇重 為4〜12重量。/〇 ; 里1/〇,且宜為2〜15重量％，更宜 金雀素黃_員：〇 宜為1〜10重量％; $里/〇,且宜為1〜15重量％，更 5 10 15 20 黃旦素黃酮類：1〇〜5〇重 宜為25〜35重量％。 董/()，且宜為15〜35重量％，更 該含雌馬酚之大豆 之組成的^軸發酵物含有習知技術無法實現 ㈣異頁嗣，因此装 之異黃_含有物晰 、j T換言為具有前述異黃酮組成 製造具有前述組成之里龙 酵物時，特別適合使用乳^的含雌馬狀大豆胚軸發 為雌馬酚產生微生物。 〇_92(FERM ΒΙΜ°036號)作 轴之皂# Α雌’物之大戏㈣酵物亦具有源自大豆胚 ^大雌馬盼之大豆胚㈣酵物中之皂扣含雕馬 2·胚軸發酵物每 ㈣，，且宜為2〇5〇⑽重A計，所綱為 且為20〜5Gmg’更宜為3()〜4〇叫。 此外，如刖所述，使用鳥胺酸•雌 p胺酸添加於大豆胚軸再進行發料獲彳 =轴發酵物中含有鳥胺酸。此種含雌馬一轴 ^所含鳥«之含量，具體來說可例示如：含雌馬紛 h大絲軸發酵物的每乾燥重4lg，鳥胺酸佔5〜2呵，且 且為8〜15mg ’更宜為9〜12mg程度。 以前述條件發酵處理而獲得之含雌馬酚之大豆胚軸發 酵物可直L雜之狀態供予本發明之製造方法，但亦 25 200906315 可依需要供予乾燥處理製成乾燥固態物狀後再供至本發明 之製造方法。 此外，以前述條件發酵處理獲得之含雌馬酚之大豆胚 軸發酵物亦可於後述之第1-1步驟前先供予脫脂處理等之前 5 處理。脫脂處理可藉由萃取溶劑使用己烷之處理來進行。 1-1.第I方法之製造方法 第I法之製造方法中，係藉由進行下述第1-1步驟來進行 含雌馬酚之萃取物的製造。茲就第I法之製造方法詳述於 下。 10 第1-1步驟 本第I方法之含雌馬酚之萃取物製造方法包含：第1-1 步驟，係使用乙醇水溶液作為萃取溶劑，將含有雌馬酚之 大豆胚軸發酵物進行萃取處理，並回收萃取液。 本第1-1步驟所使用之乙醇水溶液（乙醇與水之混合液) 15 可列舉如··乙醇濃度佔20〜98容量％，且宜95〜45容量％，更 宜85〜85容量％。藉由滿足此種濃度範圍，可從含有雌馬酚 之大豆胚軸發酵物中有效率地萃取包含雌馬酚之有用成 分。 第1-1步驟之萃取處理中，舉例來說，含雌馬酚之大豆 20 胚軸發酵物（乾燥重量換算）每1重量份可使用乙醇水溶液 1〜1000重量份（宜2〜500重量份，更宜為5〜20重量份）。 第1-1步驟中萃取處理之處理條件並未特別受限，但可 藉冷浸、溫浸等浸潰法、滲透法等進行。具體來說，於含 雌馬酚之大豆胚轴發酵物加入乙醇水溶液，以常溫1分鐘以 26 200906315 上（宜常溫5分鐘〜48小時）進行靜置或攪拌即可。 馬盼^進行萃取處理後，藉由回收萃取液即可獲得含雌 心分離方:體來說，萃取液之回收可採用過遽及離 5 10 15 20 酮類步驟回收之萃取液含有雌馬紛及黃豆素黃 低。如前述第]Γ ’含雌秘之大豆胚軸發酵物所含油分降 溶劑製成叫:Γ所得萃取液™x餘或除去萃取 t。此外十/含雕馬齡之萃取物配合於食品或藥品 除萃取溶劑步驟所得萃取液亦可直接以液狀或去 於Α 成固狀而供予雌馬酚之純化。 酮類收之萃取液中，除了雌馬盼及黃豆素黃 等不快氣味=日Γ有料，其係造顏味、苦味、唆味 有用成分的濃;:為了去除該急普並提高包含雖馬齡之 ^ ^第1-1步驟所回收之萃取液宜更供予後述 〈弟1-2步驟。 蓋Ιι2_步驟 第1-2步驟係由 後，使用乙醇作為革:1-1步驟所得萃取液去除萃取溶劑 取液。 馬卒取溶劑使其進行萃取處理’並回收萃 於第1-2步驟φ at „ ，可藉由乾燥處理等習知方法，從第1-1 ，所得萃取^去料取溶劑。 醇作為萃取溶劑，將從第1-1少驟所得萃取液去 %卒取溶劑而獲搵+ η〜 堆之固態物（以下’有時記為「原料固態物」） %行卒取處理。笛T q 乐12步驟中作為萃取溶劑使用之乙醇係未 27 200906315 添加水之乙醇，亦可為吸收有無法避免之水蒸氣者。 第1-2步驟之萃取處理中，舉例來說，原料固態物(乾燥 重量換算）每1重量份可使用乙醇1〜1000重量份，且宜為 5〜500重量份，更宜為5〜20重量份。 5 第1-2步驟中之萃取處理之處理條件亦未特別受限，可 以冷浸、溫浸等浸潰法、滲透法等來進行。具體來說，於 原料固態物加入乙醇，以常溫1分鐘以上（宜常溫5分鐘〜48 小時)進行靜置或攪拌即可。此外，亦可於第1-2步驟之萃取 處理中進行超音波處理，可藉此提高雌馬酚之回收率。 10 如此進行萃取處理後，可藉由回收萃取液而進一步獲 得含雌馬酚之萃取物。具體來說，萃取液之回收可採用過 濾或離心分離等習知方法。 如此於第1-2步驟回收之萃取液因包含雌馬酚及黃豆素 黃酮類之有用成分比例提高且皂苷含量減低，與第1-1步驟 15 所得萃取液相較下，其有用性更提高。前述第1-2步驟所得 萃取液可直接以液狀或去除萃取溶劑製成固態狀而作為含 雌馬紛之萃取物，配合至食品或藥品中。此外，前述第1-2 步驟所得萃取液亦可直接以液狀或去除萃取溶劑製成固態 狀而供予雌馬酚之純化。另，從提高含雌馬酚之萃取物的 20 保存安定性之觀點來看，第1-2步驟所得萃取液宜去除萃取 溶劑製成固態狀。 第I方法所得含雌馬酚之萃取物的組成及用途 前述第1-1步驟或第1-2步驟所得萃取液中，以其乾燥固 態部分之總量（1〇〇重量％)計，平均含有雌馬酚0.2〜40重量 28 200906315 %(宜0.4〜24重量％，更宜1〜18重量。/、 , „ 重/〇)。此外，前述第1-1步 驟或第叫賴得萃取液中1其乾燥固㈣份之總量 計，平均含有黃豆素黃酮嶋1〜8重量％(物卜5重量％， 更宜0_2〜3重量％)。 5 前述扣步料訂2㈣轉切馬敎萃取物含 高濃度雌馬酴，可表現基於雌馬盼之有用活性。具體來說， 前述第w步㈣叫㈣簡切㈣之萃取物對於預 防及改善更年期障礙、骨質疏鬆症、前列腺肥大、代謝症 候群_械synd_)等疾病或症狀、低減血中膽固醇 H)值、美白、改善挫瘡、整腸、改善肥胖以及利尿等上甚為 有用。由於表現出此種有用活性，前述第^步驟或第^步 驟所得含雌馬盼之萃取物可配合至食品或藥品中加以使 用。 將前述第1_1步驟或#2步驟所得含雌秘之萃取物 b添加至食品來使用時，舉例來說，該含雌馬紛之萃取物可 配合至飲料、顆粒、細粒、膠囊、錠劑、粉末、乳製品、 口香糖(gum)、勝狀點心(g_y)、布丁、條狀點心㈣及 其他固態食品等。配合該含雌馬齡之萃取物的食品除了可 用作—般食品外，可作為特定保健用食品、營 2〇機能性食品、病患食品等。含有本發明大豆胚轴發酵:之 食品特別是作為營養輔助食品甚有用處。此外，就含有前 述第1-1步驟或第1-2步驟所得含雌馬酚之萃取物的食品而 言’具體例係如後述。 將第1-1步驟或第〗-]步驟所得含雌馬酚之萃取物配合 29 200906315 於食品時，該食品中之該含雌馬酚之萃取物的配合比例可 依照該食品之種類、雌馬酚含量、攝取對象之年齡及性別、 所期待之效果等來加以適當設定。舉一例來說，相對於前 述食品100g，含雌馬酚之萃取物（乾燥重量換算）以總量計係 5 可佔0.1〜90g的比例。 此外，將第1-1步驟或第1-2步驟所得含雌馬酚之萃取物 配合至藥品時，該含雌馬酚之萃取物可調製成錠劑、丸劑、 粉劑、液劑、懸浮劑、乳劑、顆粒劑、膠囊劑、栓劑等形 態之藥品。配合有該含雌馬酚之萃取物的藥品作為更年期 10障礙(更年期不定之違和、骨質疏鬆症、高血脂症）、骨質疏 鬆症、W列腺肥大、代謝症候群等疾病或症狀之預防或改 善劑、血中膽固醇值之減低劑、整腸劑、肥胖改善劑、利 尿劍等甚為有用。特別是，含有該含雌馬盼之萃取物的藥 品適合使用在預防或治療中高年齡女性更年期不定之違和 15以及伴隨停經之症狀(如骨質疏鬆症、更年期障礙等）上。 至於配合有第1-1步驟或第1-2步驟所得含雌馬酚之萃 取物的藥品投藥虽，係依含雌馬酚之萃取物中的雌馬酚含 量、投藥對象之年齡及體重、症狀、投藥次數等而異，無 法統一規定，舉例來說，成人1日平均投藥量可相當於：含 20雌馬酚之萃取物以乾燥重量換算計係1〜200〇mg(且宜 5〜lOOOmg，更宜40〜50mg)的量。特別是，可將含雌馬酚之 萃取物的每日投藥量設定成投予2〜3〇mg之雌馬酚量。 1_2.第II方法之製造方法 第II方法之製造方法係藉由進行下述第IM#驟及第 3〇 200906315 II-2步驟來製造含雌馬盼之萃取物。兹就郎方法之製造方 法詳述於下。 第II-1步驟 於本第II方法之製造方法中，首缺含雌秘之大豆胚 5軸發酵物料於水並添加酸_整至pH2〜6，並时不溶物 (第II-1步驟）。換言之’本跡丨步_於含㈣之水溶液 (pH2 6)中使含雌馬紛之大豆胚軸發酵物懸浮，並回收不溶 物。 10 15 20 於本跡i步驟中，係使含雌騎之大豆胚轴發酵物懸 子於水，再加入酸而調整至PH2〜6。 本第H-1步驟所添加之酸可為有機酸或無機酸。有機酸 之種舰㈣魅料PH制祕，縣_受到限制， 可列舉如:捧樣酸、-酒石酸、富馬酸、L-抗壞血酸、葡 糖酸、=、乳酸、DL_蘋果酸、草酸1酸、两酸、赂酸 =績酸等。料，無鑛之種触可難至 鱗酸等。料酸之中，以乙酸、魏、硫酸為佳。 ^驟中’使用在使含雌馬齡之大豆 之大-發：: i份，份，且宜^重量 於第II-1步驟中 之大豆胚軸發酵物， 時)靜置或攪拌即可。 ，係於PH2〜6之水溶液中混合含雕馬紛 以室溫30分鐘以上（宜常溫2小時〜^小 31 200906315 如此處理後，因雌馬酚殘存於不溶物中，可藉由回收 不溶物而回收雌馬酚。具體來說，不溶物之回收可藉過濾 或離心分離等習知方法進行。 前述第II-1步驟所得不溶物係供至以下之第II-2步驟。 5 第II-2步驟 對前述第Π-1步驟所得不溶物使用乙醇水溶液作為萃 取溶劑以進行萃取處理，並回收萃取液（第II-2步驟）。 前述第II-1步驟所得不溶物無論是乾燥物或未經乾燥 物均可供予本第II-2步驟。然而，使用不溶物之未乾燥物 10 時，宜測定水分含量並考慮該水分量來調整後述之乙醇水 溶液的水分量。 本第Π-2步驟所使用之乙醇水溶液（乙醇與水之混合液) 可列舉如乙醇濃度50〜95容量％(宜70〜90容量％)。藉由滿足 此種濃度範圍，可從前述第H-2步驟所得不溶物有效率地萃 15 取出含雌馬酚之有用成分。 舉例來說，前述第II-1步驟所得不溶物（乾燥重量換算） 每1重量份可使用乙醇水溶液2〜30重量份（宜4〜10重量份） 以進行第II-2步驟中之萃取處理。 第II-2步驟實施之萃取處理之處理條件並未特別受 20 限，可以冷浸、溫浸等浸潰法、滲透法等進行。具體來說， 於前述第II-1步驟所得不溶物中加入乙醇水溶液，以常溫30 分鐘以上（宜常溫1〜50小時）進行靜置或攪拌即可。 如此進行萃取處理後，藉由回收萃取液即可獲得含有 雌馬酚之分液。具體來說，萃取液之回收可藉過濾或離心 32 200906315 分離等習知方法進行。 10 ο前述第H-2步驟所回收之萃取液由於雌馬㈣濃縮至 局濃度’且更含有黃1素黃_等有用成分，*含雌馬盼 之大豆胚軸發酵物相較下有用性更提高。前述第n-2步驟所 得萃取液可直接叫狀或錢萃取溶㈣絲末狀而作為 含雌馬紛之萃取物，配合至食品或藥品中。此外，前述第 Π-2步驟簡萃取液亦可直如錄或去料取溶劑製成 粉末狀而更供予雌馬紛之純化1，從提高含雌馬盼之萃 取物的保存安定性之觀點來看，第π·2步驟所得萃取液宜去 除萃取溶劑製成粉末狀。另外，可藉前述第！方法之第！-2 步驟中之絲溶咖同的條件來實施從第Π·2步驟所得萃 取液去除萃取溶劑，且轉儒賴法進行。 ^ j述第11方法所得萃取液（前述步驟之第II-2步驟所 15得萃取液)乾燥，可藉此以9G%以上之回收率回收雖馬齡， 而製得相對於含雌馬紛之大豆胚軸發酵物係含10倍量程度 濃度的含雌馬紛之粉末。如此製得之含雌馬盼之粉末因雖 馬齡/辰度提阿，具有可更有效表現基於雖馬紛之有用活性 的優點。前述第11方法所得含雌騎之粉末的用途及使用方 20法等，係與前述第Ϊ方法所得含雌馬紛之萃取物相同。 2·雖馬盼之純化方法 此外，本發明提供一種從雌馬盼含有物純化出雌馬齡 -體來4本發明之雌馬盼純化方法可大別為後 述之第1方法及第2方法物手法。兹就供予本發明純化方 33 200906315 =雕騎含麵以及第1方法及第2方法的具體内容詳述 继A酚含有物 僅兩人月之純化方法中’供純化處理之雌馬紛含有物 雌馬㈣可，並未特別受限，可為藉化學合成法 而^成有雌馬盼之反應產物，亦 馬齡之發酵物。發酵、…一，、、' 糟發酵法而產生有雌 雌馬酚姓槿心’斤侍3雌馬酚之發酵物含有多種與 二，之異黃明，但本發明之純化方法 10 15 20 =二=_馬粉之優點一優點， 含雌馬盼之發酵物:之雌馬紛含有物的-較佳例可列舉如 茲就含雌馬酚之發酵物說明如下。 而製lilt馬酴產生微生物並依照習知方法進行發酵， 物，即具有將木f素黃咖=體錢，將雌騎產生微生 素黃綱及二f MU Μ素黃酮配糖體、木質 產生雌_ _所構成群組巾之至少1種）資化而 黃：Γ能力(代謝活性)的微生物接種於含該木質辛 酵原料(供發酵之原料)，再 2 =酵(培養)’即可製得含•馬盼之發酵物。 别述雌馬酚產生微生物 取物的製造方法」中「含雌民』34 l含雌馬盼之萃 所載之雌，一產生微生物/社A豆帅發酵物」—攔 類即可，並貝素―類之發酵原料僅需含木質素黃_ 並未特別受限’值從安全性之觀點來看，以可： 34 200906315 作食品素材者為宜。具體來說，含木質素黃酮類之發酵原 料可列舉如大豆、大豆胚軸、大豆胚軸之萃取物、豆腐、 炸豆腐、豆乳、納豆、醬油、味嗜、天貝（tempe)、紅花苜 蓿（red clove)或其萃取物、紫苜蓿或其萃取物等。該等之 5 中，由於大豆胚軸含有豐富木質素黃酮類，而宜作為含木 質素黃酮類之發酵原料。 此外，含木質素黃酮類之發酵原料更可添加含前述木 質素黃酮類之異黃酮。如前述，藉由預先另行添加異黃酮 於發酵原料中，可更提高所得發酵物中之雌馬酚含量。 10 再者，含木質素黃酮類之發酵原料可以促進發酵效率 等之目的而依需要添加酵母萃取、聚蛋白脒、肉精等氮源； 葡萄糖、蔗糖等碳源；磷酸鹽、碳酸鹽、硫酸鹽等無機鹽； 維生素類；胺基酸等營養成分。 製造含雌馬酚之發酵物時，發酵原料之水分量、發酵時間、 15 發酵溫度、發酵環境等發酵條件依雌馬酚產生微生物之種 類、發酵原料種類、雌馬酚產生量、木質素黃酮類殘存量 等加以適當設定即可。 本發明純化方法所使用之雌馬酚含有物宜為前述「1. 含雌馬酚之萃取物的製造方法」一欄所載之含雌馬酚之大 20 豆胚轴發酵物。 2-1.第1方法之純化方法 第1方法之純化方法係藉由進行下述第1-1步驟〜第1-5 步驟來進行雌馬酚之純化。茲就第1方法之純化方法詳述於 下。 35 200906315 第1-1步驟 本第1方法之純化方法係先使用乙醇水溶液作為萃取 溶劑，將雌馬酚含有物進行萃取處理並回收萃取液（第1-1 步驟）。 5 舉例來說，本第1-1步驟所使用之乙醇水溶液可列舉 如：乙醇濃度為20〜98容量％，且宜為45〜95容量％，更宜為 45〜80容量％。藉由滿足此種濃度範圍，可有效率地從雌馬 酚含有物中萃取雌馬酚。 第1-1步驟之萃取處理中，舉例來說，含雌馬酚之大 ίο 豆胚軸發酵物（乾燥重量換算）每1重量份可使用乙醇水溶液 1〜1000重量份（宜2〜500重量份，更宜為5〜20重量份）。 第1-1步驟中萃取處理之處理條件並未特別受限，但 可藉冷浸、溫浸等浸潰法、滲透法等進行。具體來說，於 含雌馬酚之大豆胚軸發酵物加入乙醇水溶液，以常溫1分鐘 15 以上（宜常溫5分鐘〜48小時）進行靜置或攪拌即可。 如此進行萃取處理後，藉由回收萃取液即可獲得含雌 馬酚之萃取物。具體來說，萃取液之回收可採用過濾及離 心分離等習知方法。 於第1-1步驟回收之萃取液中，除了雌馬酚及黃豆素黃 20 酮類以外，更同時含有黃豆素黃酮類等異黃酮及皂苷。 前述第1-1步驟所回收之萃取液將供予後述之第1-2步 驟。 第1-2步驟 由第1-1步驟所得萃取液去除萃取溶劑後，對所得殘留 36 200906315 物使用乙醇作為萃取溶劑進行萃取處理，並回收萃取液（第 1-2步驟）。 於本第1-2步驟中，從第1-1步驟所得萃取液去除萃取溶 劑、以乙醇對所得殘留物進行萃取處理以及萃取液之回收 5 等，均以與前述「1.含雌馬酚之萃取物的製造方法」之第I 方法中之第1-2步驟相同的條件實施。 前述於第1-2步驟回收之萃取液的皂苷含量減低，但除 了雌馬酚以外，亦含黃豆素黃酮類等之異黃酮。 前述第1-2步驟所得萃取液將供予以下第1-3步驟。 10 第1-3步驟 從前述第1-2步驟所得萃取液去除萃取溶劑後，使所得 殘留物懸浮於己烷，並回收不溶物（第1-3步驟）。 於第1-3步驟中，可藉減壓蒸餾、乾燥等習知方法，從 第1-2步驟所得萃取液去除萃取溶劑。 15 對從第1-2步驟所得萃取液去除萃取溶劑而獲得之殘 留物（以下，有時將其記為「第2原料殘留物」）添加己烷並 使其懸浮。 就第1-3步驟中之己烷添加量而言，舉例來說，第2原 料殘留物（乾燥重量換算）每1重量份可使用己烷1〜50重量份 20 (宜5〜10重量份）進行添加。 於第1-3步驟中，將第2原料殘留物懸浮於己烷之處理 條件亦未特別受限，舉例來說，可藉冷浸、溫浸等浸潰法 等來進行。具體而言，於第2原料殘留物中添加己烷，以常 溫5分鐘以上（宜常溫3分鐘〜1小時）進行靜置或攪拌即可。 37 200906315 如此於己烷中進行懸浮後，因雌馬酚不溶化，可藉由回收 不溶物而獲得含雌馬酚之固態成分。具體而言，不溶物之 回收可以過濾及離心分離等習知方法進行。 就由實施前述第1-3步驟，而從第1-2步驟所得萃取液中 5 去除脂溶性之夾雜物質。第1-3步驟所得不溶物將供至以下 第1-4步驟。 第1-4步驟 對前述第1-3步驟所得不溶物使用己烷與醚之混合液 作為萃取溶劑進行萃取處理，並回收萃取液（第1-4步驟）。 10 於第1-4步驟中，就作為萃取溶劑的成分之一來使用之 醚而言，其種類並未特別受限，但可列舉如：二曱醚、曱 基乙基醚及二乙醚等。該等醚可使用單一種，亦可組合2種 以上使用。從有效率地純化雌馬酚的觀點來看，前述醚之 中，以二乙醚為宜。 15 此外，第1-4步驟使用之己烷與醚之混合液中，己烷與 醚之混合比並未特別受限，但從提高所純化之雌馬酚純度 的觀點來看，可例示如：己烷與醚之容量比為10 : 90〜20 : 80，且宜為30 : 70〜25 : 75。 舉例來說，前述第1-3步驟所得不溶物（乾燥重量換算) 20 每1重量份可使用己烷與醚之混合液50〜5000重量份（宜 200〜1000重量份）來進行第1-4步驟中之萃取處理。 第1-4步驟實施之萃取處理之處理條件亦未特別受 限，舉例來說，可藉冷浸、溫浸等浸潰法、滲透法等來進 行。具體來說，於前述第1-3步驟所得不溶物中加入己烷及 38 200906315 醚之混合液，以常溫Η)分鐘以上（宜常溫⑽鐘〜料時程 度）進行靜置或攪拌即可。 如此進行萃取處理後，藉由回收萃取液可更獲得雕馬 酚。具體來說，萃取液之回收可採用過渡及離心分離等習 5 知方法。 第1-5步驟 將前述第Μ㈣所得萃取液供予：氧切凝膠層 析，回收含_馬狀分液（第丨_5步驟）。 本第1-5步财之二氧切郷管㈣衫要是將使 川用二氧化石夕凝膠作為充填劑之管柱用作分離床㈣麵㈣ 的層析即可，可為任-樣式。該二氧化石夕凝膠管柱層析可 具體列舉如：使溶劑以重力落下而流送之開管式層析⑴卿More suitable for 38 ~ 耽 can be listed as 2 〇 ~ machine, should be H Fermentation time can be based on the amount of female horses, lignin yellow _ class residuals, 22 200906315 female horse-born nuclear life (four) 曰 且It is 2~7曰, more preferably 3~5曰. ~ usually 1~1〇3 phenol production 4 ^ conditional scale treatment (four) large-scale fermentation of the shaft of the horse age and accumulation, can be expressed in the male horse fermented material, although the equol in the hypocotyl fermented product contains strontium . It depends on the soybean organism and the fermentation conditions, etc.:: The second line is based on the average dry weight of the estrogen age (the size is large: and the 5' with the large five-wheeled fermented product has _mg, and ^ The dry weight of 21 turns is (4)), and the large post containing the female horse age is more preferably 5. 10 15 20 In addition, it also contains: Soybean*, B. bismuth::: flavonoids and 4 lignin yellow (4) wood 峨 (10) ^ The composition is recorded as "lignin yellow _"); dye handle (genistm) 1 Ginkgo biloba, B. sinensis, B. sylvestris, B. sylvestris, sylvestre, etc. (hereinafter referred to as "Golden Finch = Class") Lycitin), B. sinensis Various isoflavones such as soy jaundice, dimethicone, yellow crocetin, etc. (hereinafter referred to as "n-yellow yellow _"), and may also exhibit useful physiology of such isoxanthin active. The content of isoflavones (including esculin) in the fermented soybean hypocotyls containing the equine horse varies depending on the estrogen microorganisms and fermentation conditions used, but in general, it can be exemplified as: The dry weight of the large shaft shaft fermented product, the isoflavone is 5 to 20 mg, and preferably 5 to 1.5 mg, more preferably 8 to 15 mg. In addition, the soy embryonic fermented product containing the equestrian horse is secreted in the same way as the soybean embryo. 23 200906315 In particular, the total content of the genistein flavonoids in the equol-containing soybean hypocotyl fermentation is as low as 15% by weight based on the total isoflavones of the equol-containing soybean hypocotyl ferment. Below /Q, it is preferably 12% by weight or less; from the viewpoint of isoflavone composition, it is more advantageous than the soybean hypocotyl before fermentation. In addition, the golden scorpion 5 scutellariae is an isoflavone that is concerned about acting as an endocrine disrupting substance, and the concentration in the food material is preferably reduced as much as possible (Young HJ, Jodi F, Kimberly FA, Daniel RD, William GH. Effects of dietary daidzein and its metabolite, equol, at physiological concentrations on the growth of estrogen-dependent human 10 breast cancer (MCF-7) tumors implanted in ovariectomized athymic mice. Carcinogenesis 127(4): 856-863, 2006 reference). Specifically, the equol-containing soybean hypocotyl fermentate can be exemplified by the following isoflavone composition (the unit "mg" below represents the total amount of each isoflavone per 1 g (dry weight) of the soybean hypocotyl fermentate): 15 Equine: 1~20mg, preferably 2~I2mg Lignin flavonoids: 0.1~3〇mg, preferably 〇1~l 5mg Ginkgo biloba: 0.05~2.5mg, preferably 〇〇5~ 2 tons of daidzein flavonoids: 〇_1~4mg, preferably 2~3 5mg In addition, the composition ratio of each isoflavone contained in the equol-containing soybean hypocotyl 1 transcript is exemplified as the following range (hereinafter The unit "% by weight table _ = all the two or two contained in the fermented soybean hypocotyls containing the female horses; ratio": preferably 40 to 70% by weight, more preferably 45 to 70 equol: 30 to 75 % by weight, weight. / 〇; 24 200906315 lignin yellow _ class: 1~2 〇 weighs 4~12 weight. / 〇; 里 1 / 〇, and preferably 2~15% by weight, more suitable for ginseng yellow _ member: 〇 should be 1~10% by weight; $里/〇, and preferably 1~15% by weight, more 5 10 15 20 huangfangsu flavonoids: 1〇~5〇 weight should be 25~35wt% Dong / (), and should be 15 to 35% by weight, and the composition of the equol-containing soybean is not known to be known by the prior art. (4) The heterologous yttrium is contained, so the yellowish _ contains the substance, and the J is in other words has the aforementioned isoflavones. When the composition of the Lilong protein having the above composition is formed, it is particularly suitable to use the female horse-like soybean hypocotyls of the milk to produce equol-producing microorganisms. 〇_92 (FERM ΒΙΜ°036) as the axis soap #Α女' The drama of the matter (4) The yeast also has a soybean embryo derived from the soybean embryo, and the soap is contained in the yeast. The fermented horse 2 is the fermented product of the hypocotyl per plant (4), and should be 2〇5〇(10). The formula is 20~5Gmg' is more preferably 3()~4〇. In addition, as described in 刖, the use of ornithine and estrogen is added to the soybean hypocotyls and then the hair is obtained. The content of ornithine is contained in the product. The content of the bird contained in the one-side of the female horse can be exemplified by, for example, 4 lg per dry weight of the fermented product of the female horse and avian acid accounting for 5 ~2 Oh, and 8~15mg' is more preferably 9~12mg. The equol-containing soybean hypocotyl ferment obtained by fermentation treatment under the foregoing conditions may be The state of the L is supplied to the production method of the present invention, but also 25 200906315 can be supplied to the dry process in a dry solid state as needed, and then supplied to the production method of the present invention. The soybean hypocotyl fermented product of the p-phenol can also be subjected to the pretreatment 5 before the step 1-1 described later, etc. The degreasing treatment can be carried out by treating the extraction solvent with hexane. Method of Production of the Method In the production method of the first method, the production of the equol-containing extract is carried out by performing the following step 1-1. The manufacturing method of the first method is described in detail below. 10 Step 1-1 The preparation method of the equol-containing extract of the first method comprises the following steps: Step 1-1, using an aqueous solution of ethanol as an extraction solvent to extract the fermentation product of equol containing soybean hypocotyls And recover the extract. The aqueous ethanol solution (mixture of ethanol and water) used in the first step 1-1 is, for example, an ethanol concentration of 20 to 98% by volume, and preferably 95 to 45 % by volume, more preferably 85 to 85% by volume. By satisfying such a concentration range, a useful component containing equol can be efficiently extracted from a soybean hypocotyl fermentation product containing equol. In the extraction treatment of the first step 1-1, for example, the equol-containing soybean 20 hypocotyl fermented product (in terms of dry weight) may be used in an amount of 1 to 1000 parts by weight (preferably 2 to 500 parts by weight) per 1 part by weight of the aqueous ethanol solution. More preferably 5 to 20 parts by weight). The treatment conditions for the extraction treatment in the first step 1-1 are not particularly limited, but may be carried out by a dipping method such as cold soaking or warm dipping, an infiltration method or the like. Specifically, an aqueous solution of ethanol is added to the equol-containing soybean hypocotyl fermented product, and it is allowed to stand or stir at a normal temperature for 1 minute at 26 200906315 (preferably at a normal temperature for 5 minutes to 48 hours). After the extraction process, the extract containing the female heart can be obtained by recovering the extract: for the body, the extract can be recovered by using the hydrazine and the extract obtained from the 5 10 15 20 ketone step. There are many yellow soybeans and low in yellow. As described above, the oily fraction of the soybean hypocotyl fermented product containing the female secret is prepared by the extraction of the extract TMx or the extraction of t. In addition, the extract of the 10th/carved horse age is compounded with food or medicine. The extract obtained by the extraction solvent step can be directly supplied to the equol for purification in liquid form or in a solid state. In the ketone-receiving extract, in addition to the unpleasant odor of the female horse and the yellow bean yellow, the scent of the scent is inferior to the scent of the scent, the bitterness, and the scent of the scent; in order to remove the rush and improve the inclusion of the horse The extraction liquid recovered in the first step of step 1-1 is preferably supplied to the latter step 1-2. Step ι2_Step Step 1-2 is followed by using ethanol as the leather: the extract obtained in the 1-1 step is used to remove the extraction solvent and take the liquid. The solvent is taken from the horse to be subjected to extraction treatment and recovered in step 1-2 φ at „, and the solvent can be extracted from the extraction 1-1 by a conventional method such as drying treatment. The solvent is obtained by removing the solvent from the first to the first 1-1 and extracting the solvent to obtain the solid matter of the 搵+η~ heap (hereinafter referred to as "the raw material solid"). Ethanol used as an extraction solvent in the step of flute T q Le 12 is not added. 27 200906315 Ethanol added with water may also be used to absorb water vapor which is unavoidable. In the extraction treatment of the first step, for example, the solid material (in terms of dry weight) may be used in an amount of from 1 to 1000 parts by weight, and preferably from 5 to 500 parts by weight, more preferably from 5 to 20 parts per 1 part by weight of the ethanol. Parts by weight. The treatment conditions of the extraction treatment in the first step 1-2 are also not particularly limited, and may be carried out by a dipping method such as cold soaking or warm dipping, a permeation method or the like. Specifically, ethanol is added to the solid material of the raw material, and it is allowed to stand or stir at a normal temperature for 1 minute or more (preferably at a normal temperature for 5 minutes to 48 hours). Further, ultrasonic treatment can be carried out in the extraction treatment in the first step 1-2, whereby the recovery of equol can be improved. 10 After the extraction treatment, the extract containing equol can be further obtained by recovering the extract. Specifically, the recovery of the extract may be carried out by a conventional method such as filtration or centrifugation. The extract thus recovered in the first step 1-2 has a higher ratio of useful components including equol and flavonoids and a reduced saponin content, and is more useful than the extraction liquid phase obtained in the first step 151. . The extract obtained in the above step 1-2 can be directly solidified in a liquid form or in an extraction solvent, and can be incorporated into a food or a drug as an extract containing a female horse. Further, the extract obtained in the above step 1-2 may be directly purified in the form of a liquid or a solvent to remove the extraction solvent to supply equol. Further, from the viewpoint of improving the preservation stability of the equol-containing extract 20, the extract obtained in the first to 1-2 steps is preferably removed from the extraction solvent to form a solid. The composition and use of the equol-containing extract obtained by the first method, the extract obtained in the above step 1-1 or the first step, in the total amount of the dried solid portion (1% by weight), average Containing equol 0.2~40 weight 28 200906315% (preferably 0.4~24% by weight, more preferably 1~18 weight. /, , „重/〇). In addition, the aforementioned step 1-1 or the first call of Laid extract The total amount of the dried solid (four) parts of the medium 1 contains, on average, 1 to 8 wt% of the soy flavonoids (5 wt%, more preferably 0 2 to 3 wt%). 5 The aforementioned deductions are ordered 2 (four) to cut the horses The extract contains a high concentration of estrogen, which can be expressed based on the useful activity of the female horse. Specifically, the above step (4) is called (4) the simple (4) extract for preventing and improving menopausal disorders, osteoporosis, prostatic hypertrophy, Metabolic syndrome _ machinery synd_) and other diseases or symptoms, low blood cholesterol C) value, whitening, improvement of contusion, intestine, improvement of obesity and diuresis, etc.. Because of this useful activity, the aforementioned steps Or the extract containing the espresso obtained in the second step can be blended into food or medicine When the extract containing the female extract obtained in the above step 1_1 or #2 is added to the food, for example, the extract containing the female horse can be blended into the beverage, the granule, the fine granule, and the capsule. , lozenges, powders, dairy products, gums, scented snacks (g_y), puddings, strips of snacks (4) and other solid foods, etc., in addition to the foods containing the extracts of female horses, In addition to food, it can be used as a food for specific health care, a functional food for a ban, a food for a patient, etc. The food containing the soybean hypocotyl fermentation of the present invention is particularly useful as a nutritional supplement food. The specific example of the food containing the equol-containing extract obtained in the first step or the second step is as follows. The equol-containing extract obtained in the first step or the first step is combined with 29 200906315 In the case of a food, the blending ratio of the equol-containing extract in the food may be appropriately set depending on the type of the food, the equol content, the age and sex of the ingested subject, the desired effect, and the like. Relative to 100 g of the above-mentioned food, the extract containing equol (calculated as dry weight) may be in a ratio of 0.1 to 90 g in total. Further, the equol-containing product obtained in the first step or the second step When the extract is compounded into a medicine, the equol-containing extract can be adjusted into a tablet, a pill, a powder, a liquid, a suspension, an emulsion, a granule, a capsule, a suppository, etc. A drug for extracts of equine as a preventive or ameliorating agent for diseases or symptoms such as menopausal disorder 10 (menopausal disorder, osteoporosis, hyperlipidemia), osteoporosis, W-line gland hypertrophy, metabolic syndrome, blood cholesterol Value reduction agents, colon preparations, obesity improvers, diuretic swords, etc. are very useful. In particular, the drug containing the extract containing the esculentus is suitable for use in the prevention or treatment of menopausal irregularities in high-age women and 15 and symptoms associated with menopause (e.g., osteoporosis, menopausal disorders, etc.). The pharmaceutical product containing the equol-containing extract obtained in the 1-1st step or the 1-2th step is administered according to the equol content of the equol-containing extract, the age and body weight of the administration target, The symptoms and the number of administrations vary, and cannot be uniformly defined. For example, the average daily dose of an adult can be equivalent to: the extract containing 20 equol is 1 to 200 〇mg in dry weight conversion (and preferably 5~) The amount of lOOOOg, more preferably 40~50mg). In particular, the daily dose of the equol-containing extract can be set to a dose of 2 to 3 mg of equol. 1_2. Method for Producing Method II The method for producing the method of the second method is to produce an extract containing equuma by performing the following IM# and the third step 200906315 II-2. The manufacturing method of the Lang method is detailed below. Step II-1 In the manufacturing method of the second method, the first-order soybean embryo 5-axis fermentation material is firstly dehydrated and added with acid _ to pH 2 to 6, and insoluble matter (step II-1) . In other words, the original soy hypocotyl fermented product was suspended in an aqueous solution (pH 2 6) containing (4), and insoluble matter was recovered. 10 15 20 In the i step of the present trace, the soybean hypocotyl fermented product containing the female rider is suspended in water, and acid is added to adjust to pH 2-6. The acid added in the step H-1 may be an organic acid or an inorganic acid. Organic acid species (four) charm material PH secret, county _ restricted, can be listed, such as: holding acid, - tartaric acid, fumaric acid, L-ascorbic acid, gluconic acid, =, lactic acid, DL_ malic acid, oxalic acid 1 acid, two acids, acid = acid and so on. It is difficult to get scaly acid and so on. Among the acid materials, acetic acid, Wei, and sulfuric acid are preferred. In the middle of the experiment, the soybeans containing the female horses are used in a large amount:: i parts, portions, and the weight of the soybean hypocotyl ferment in the step II-1 is allowed to stand or stir. . In the aqueous solution of PH2~6, the mixed horses are mixed at room temperature for 30 minutes or more (should be normal temperature for 2 hours~^small 31 200906315). After the treatment, the equol remains in the insoluble matter, and the insoluble matter can be recovered. The equol is recovered. Specifically, the recovery of the insoluble matter can be carried out by a conventional method such as filtration or centrifugation. The insoluble matter obtained in the above step II-1 is supplied to the following step II-2. In the second step, the insoluble matter obtained in the above step Π-1 is used as an extraction solvent for extraction treatment, and the extract is recovered (step II-2). The insoluble matter obtained in the above step II-1 is either dry or not. The dried product can be supplied to the step II-2. However, when the undried material 10 of the insoluble matter is used, it is preferred to measure the moisture content and adjust the moisture content of the aqueous ethanol solution described later in consideration of the moisture content. The aqueous ethanol solution (mixture of ethanol and water) used in the step may be, for example, an ethanol concentration of 50 to 95% by volume (preferably 70 to 90% by volume). By satisfying such a concentration range, the above step H-2 can be obtained. Insoluble matter is efficiently extracted 15 A useful component containing equol. For example, the insoluble matter obtained in the above step II-1 (in terms of dry weight) may be used in an amount of 2 to 30 parts by weight (preferably 4 to 10 parts by weight) per 1 part by weight of the aqueous ethanol solution. The extraction treatment in the step II-2. The treatment conditions of the extraction treatment carried out in the second step are not particularly limited to 20, and can be carried out by a dipping method such as cold soaking or warm immersion, an infiltration method, etc. Specifically, The insoluble matter obtained in the above step II-1 is added with an aqueous solution of ethanol, and it is allowed to stand or stir at a normal temperature for 30 minutes or more (preferably at a normal temperature of 1 to 50 hours). After the extraction treatment, the extract can be obtained by recovering the extract. The separation of equol. Specifically, the recovery of the extract can be carried out by a conventional method such as filtration or centrifugation 32 200906315. 10 o The extract recovered in the above step H-2 is concentrated to a local concentration due to the equine (4) 'And more useful ingredients such as yellow 1 黄 yellow _, * soybean hypocotyl fermented product containing female horse is more useful than the next. The extract obtained in the above step n-2 can be directly called or extracted to extract (4) Silky end as a female horse The extract is compounded into a food or a medicine. In addition, the above-mentioned step Π-2 step extract can also be prepared as a powder by directly recording or removing the solvent, and is further supplied to the female horse for purification. From the viewpoint of preservation stability of the extract of the female horse, the extract obtained in the step π·2 is preferably removed from the extraction solvent to form a powder. Alternatively, it may be dissolved in the second step of the above method! The extracting solution obtained by the second step is used to remove the extraction solvent, and is carried out by the Russell method. ^ j The extract obtained by the eleventh method (the extract obtained in the second step of the above step 15) Drying can be carried out by recovering the horse-aged product at a recovery rate of 9 G% or more, and producing a powder containing a female horse with a concentration of 10 times the concentration of the soybean hypocotyl fermented product containing the female horse. The powder containing the emamus which is thus obtained has the advantage of being more effective in performance based on the useful activity of the horse because of the age of the horse. The use of the powder containing the female rider obtained by the above-mentioned first method, and the method of using the same are the same as those of the extract containing the equine horse obtained by the above-mentioned second method. 2. The purification method of Mapan In addition, the present invention provides a method for purifying an estrogen-derived body from an equestrian preparation, and the method of purifying the eume is expected to be the first method and the second method described later. Things. For the purification of the present invention 33 200906315 = The surface of the carcass and the details of the first method and the second method are detailed in the purification method of the A phenol-containing material for only two months. The female horse (4) can be, but is not particularly limited, a chemical reaction method for the reaction product of the female horse, and the fermented product of the horse. Fermentation, ... a,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, = two = _ the advantage of horse powder, the advantage of containing the fermented product of the female horse: the female horse contains the contents - a preferred example can be exemplified as the fermentation product containing equol. The lilt horses are produced by microorganisms and fermented according to a conventional method, that is, having the wood, the yellow tea, the body money, the female rider produces the microbial yellow and the f MU flavonoid glycoside, wood Producing at least one species of the group _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The fermented product containing Ma Ying can be obtained. "Including the method for producing equol-producing microbial extracts", "including females" 34 l contains the females contained in the extracts of the female horses, and the microorganisms/shes A bean handsome fermented materials--blocks, and Besin-like fermentation raw materials only need to contain lignin yellow _ is not particularly limited' value from the point of view of safety, can be: 34 200906315 for food materials is appropriate. Specifically, the lignin-containing fermentation material may, for example, be soybean, soybean hypocotyl, soybean hypocotyl extract, tofu, fried tofu, soy milk, natto, soy sauce, taste, tempe, safflower (red clove) or its extract, purpura or its extracts. Among these 5, since the soybean hypocotyl is rich in lignin flavonoids, it is suitable as a fermentation material containing lignin flavonoids. Further, the lignin-containing fermentation material may further contain isoflavones containing the aforementioned flavonoids. As described above, the equol content in the obtained fermented product can be further improved by additionally adding isoflavone to the fermentation raw material in advance. 10 Further, the fermentation raw material containing lignin flavonoids may be added with a nitrogen source such as yeast extract, polypeptone, or meat essence as needed for the purpose of promoting fermentation efficiency, etc.; carbon sources such as glucose and sucrose; phosphate, carbonate, sulfuric acid Inorganic salts such as salt; vitamins; amino acids and other nutrients. When producing a fermentation product containing equol, the fermentation component, the fermentation time, the fermentation temperature, the fermentation environment, and other fermentation conditions are based on the type of equol-producing microorganisms, the type of fermentation raw material, the amount of equol produced, and the lignin flavonoids. The residual amount of the type may be appropriately set. The equol content used in the purification method of the present invention is preferably the equol-containing large 20 bean hypocotyl fermented product contained in the above-mentioned "1. Method for producing equol-containing extract". 2-1. Purification method of the first method The purification method of the first method is carried out by performing the following steps 1-1 to 1-5 to purify equol. The purification method of the first method is described in detail below. 35 200906315 Step 1-1 The purification method of the first method is to use an aqueous ethanol solution as an extraction solvent, extract the equol content, and recover the extract (step 1-1). For example, the aqueous ethanol solution used in the first step 1-1 may be, for example, an ethanol concentration of 20 to 98% by volume, and preferably 45 to 95% by volume, more preferably 45 to 80% by volume. By satisfying such a concentration range, equol can be efficiently extracted from the equol content. In the extraction treatment of the first step 1-1, for example, the equol-containing large ε 豆 胚 胚 fermentation (in terms of dry weight) may be used in an amount of 1 to 1000 parts by weight (preferably 2 to 500 parts by weight) per 1 part by weight of the aqueous ethanol solution. The portion is more preferably 5 to 20 parts by weight). The treatment conditions for the extraction treatment in the first step 1-1 are not particularly limited, but may be carried out by a dipping method such as cold soaking or warm dipping, an infiltration method, or the like. Specifically, an aqueous solution of ethanol is added to the fermentation product of the equol-containing soybean hypocotyls, and the mixture is allowed to stand or stir at a normal temperature of 1 minute 15 or more (preferably at a normal temperature for 5 minutes to 48 hours). After the extraction treatment as described above, an extract containing equol can be obtained by recovering the extract. Specifically, the recovery of the extract can be carried out by a conventional method such as filtration and centrifugation. In addition to equol and daidzein 20 ketone, the extract recovered in the first step 1-1 contains isoflavones such as flavonoids and saponins. The extract recovered in the above step 1-1 will be supplied to the first step 1-2 which will be described later. Step 1-2 After removing the extraction solvent from the extract obtained in the first step 1-1, the obtained residue 36 200906315 is subjected to extraction treatment using ethanol as an extraction solvent, and the extract is recovered (steps 1-2). In the first step 1-2, the extraction solvent is removed from the extract obtained in the first step 1-1, the residue is extracted by ethanol, and the extract is recovered, 5, etc., and the above "1. Containing equol The same conditions as in the first to third steps of the first method of the method for producing the extract are carried out. The saponin content of the extract recovered in the above steps 1-2 is reduced, but in addition to equol, isoflavones such as flavonoids are also contained. The extract obtained in the above steps 1-2 will be subjected to the first steps 1-3. 10 Step 1-3 After removing the extraction solvent from the extract obtained in the above step 1-2, the resulting residue is suspended in hexane, and insoluble matter is recovered (steps 1-3). In the first to third steps, the extraction solvent can be removed from the extract obtained in the first to 1-2 steps by a conventional method such as distillation under reduced pressure or drying. 15 The residue obtained by removing the extraction solvent from the extract obtained in the first step (hereinafter, referred to as "the second raw material residue") is added with hexane and suspended. For the amount of hexane added in the first to third steps, for example, the second raw material residue (in terms of dry weight) may be used in an amount of 1 to 50 parts by weight per 1 part by weight of hexane (preferably 5 to 10 parts by weight). ) to add. In the first to third steps, the treatment conditions for suspending the second raw material residue in hexane are not particularly limited, and for example, it can be carried out by a dipping method such as cold soaking or warm dipping. Specifically, hexane is added to the second raw material residue, and it may be left standing or stirred at a normal temperature for 5 minutes or more (preferably at normal temperature for 3 minutes to 1 hour). 37 200906315 After suspension in hexane, the equol-containing solid component can be obtained by recovering insoluble matter because equol is insolubilized. Specifically, the recovery of insoluble matter can be carried out by a conventional method such as filtration and centrifugation. By carrying out the above steps 1-3, the fat-soluble inclusions are removed from the extract obtained in the first step 1-5. The insoluble matter obtained in the first to third steps will be supplied to the following steps 1-4. Steps 1-4 The insoluble matter obtained in the above steps 1-3 is subjected to extraction treatment using a mixed solution of hexane and ether as an extraction solvent, and the extract is recovered (steps 1-4). In the first to fourth steps, the type of the ether to be used as one of the components of the extraction solvent is not particularly limited, and examples thereof include dioxane, mercaptoethyl ether, diethyl ether, and the like. . These ethers may be used singly or in combination of two or more. From the viewpoint of efficiently purifying equol, diethyl ether is preferred among the above ethers. Further, in the mixture of hexane and ether used in the first to fourth steps, the mixing ratio of hexane to ether is not particularly limited, but from the viewpoint of improving the purity of the purified equol, it can be exemplified as The capacity ratio of hexane to ether is 10: 90 to 20: 80, and preferably 30: 70 to 25: 75. For example, the insoluble matter obtained in the above steps 1-3 (in terms of dry weight) 20 may be used in an amount of 50 to 5000 parts by weight (preferably 200 to 1000 parts by weight) per 1 part by weight of the mixture of hexane and ether. Extraction treatment in 4 steps. The treatment conditions for the extraction treatment carried out in the first to fourth steps are also not particularly limited. For example, it may be carried out by a dipping method such as cold soaking or warm dipping, a permeation method or the like. Specifically, a mixed liquid of hexane and 38 200906315 ether is added to the insoluble matter obtained in the above steps 1-3, and the mixture is allowed to stand or stir at room temperature for more than 5% (suitable for normal temperature (10) minutes to material time). After the extraction treatment as described above, the engraving can be further obtained by recovering the extract. Specifically, the recovery of the extract can be carried out by conventional methods such as transition and centrifugation. Steps 1-5 The extract obtained in the above (Μ) (IV) is supplied to an oxygen-cut gel to recover a fraction containing _ horses (step 丨5). This 1-5th step of the dioxane tube (four) shirt will be used to make the column with the dioxide as a filler for the separation of the bed (four) surface (four) of the chromatography, can be any-style . The chlorine dioxide column chromatography can be specifically enumerated, for example, an open tube chromatography (1) in which a solvent is dropped by gravity.

Column Chn>_g_y)m容舰狀㈣管柱層析 ⑺ash C0lumn ch_t()graphy)、高速液體層析⑽⑹等。 15 j等之中’尤以開管式層析及快速管柱層析於卫業上容易 實施而較理想。 "從使雌馬祕化度更提高之觀點來看，本第M步驟之 二氧化石夕凝膠管柱層析所用移動層（展開溶劑）宜使用己烷 與鱗之混合液。於此，移動層所用之喊的種類並未特別受 可列舉如二甲_、甲基乙基_及二乙醚等。該等趟可 單獨使用1種’亦可組合2種以上使用。從更提高雌馬盼之 純化度的觀點來看，前述喊宜為與前述第1-4步驟之己烧與 驗的混合液所用之咖同者。本第M步驟中利用二氧化矽 凝膠管柱層析所致雌馬齡之溶出宜以梯度法進行，即將 39 200906315 前述第1-4步驟所使用之己烧與键之混合液相同組成者作 為開始=移動層，並使該^ 由如前达般使移動層中之醚比率逐漸提高，二 己=比率為一，一^ 5動層，與失雜物分離並溶出。因此，可逸過回收一 化石夕凝=柱層柯所溶出之含雌馬_又 化之雖馬盼。再藉減《德、乾燥等習知方法，從 μ5步驟所时之切馬_分液去除萃取_，即可= 爽雜物已大致去除之純化為高純度的固態狀雕馬紛。★ 10 2-2.第2方法之純化太沬 篦2-1步騍 本第2方法之純化方法中，係先使用選自於由乙萨乙 西旨、醇、丙酮、二魏、乙腈、二乙趟及甲笨所構成群組 中之至J 1種有機溶劑或其含水有機溶劑作為萃取溶劑，對 15雌馬酚含有物進行萃取處理，並濃縮所得萃取液（第2】步 驟）。 ’ 於第2-1步驟中，萃取溶劑可使用選自 Q 0夂乙酯、醇、 丙酮、二噁烷、乙腈、二乙醚及曱笨所構成群組中之至少工 種純有機溶劑，但從有效率萃取雌馬酚之觀點來看—使 20用於該等有機溶劑中加水而成之含水有機溶劑。 於此’具體而言，作為萃取溶劑使用夕a、Α 文用之别述醇可例示 如甲醇、乙醇、異丙醇、正丙醇等。 前述含水有機溶劑中之有機溶劑濃度可列舉如8〇〜99 容量％ ’且宜為90〜97容量％。藉由滿足前诚增办 月】述/農度範圍，可 40 200906315 獲得雌馬酚含有率較高之萃取液。 本第2 -1步驟所使用之萃取溶劑較佳者可列舉如含水 乙酸乙酿。 萃取之處理條件亦未特別受限，舉例來說，可以冷浸、 5 溫浸等浸潰法等進行。具體而言，於雌馬酚含有物加入萃 取溶劑，以常溫30分鐘以上（宜常溫1小時〜50小時）進行靜置 或攪拌即可。 第2-1步驟實施之萃取處理之處理條件亦未特別受 限，舉例來說，可以冷浸、溫浸等浸潰法、滲透法等進行。 10 如前述進行萃取處理後，因雌馬酚會被萃取至萃取液 中，可藉由供至過濾及離心分離等固液分離手法來回收萃 取液。接著，將所得萃取液供予減壓濃縮等濃縮手法，獲 得雌馬酚濃縮物。所得雌馬酚濃縮物將供予以下之第2-2步 驟。 15 第2-2步驟 將前述第2-1步驟所得雌馬酚濃縮物供予二氧化矽凝 膠管柱層析，獲得含雌馬酚之分液（第2-2步驟）。 於本第2-2步驟中，前述第2-1步驟所得雌馬酚濃縮物可 直接供予二氧化矽凝膠管柱層析。 20 此外，前述第2-1步驟中以含有醇之水溶性溶劑進行萃 取處理時，於濃縮萃取液後，宜更對該濃縮物加入5-10容 量倍之乙酸乙酯與等量之水混合，之後分取乙酸乙酯分液 並濃縮，供予管柱層析。 本第2 - 2步驟中之二氧化矽凝膠管柱層析只要是將使 41 200906315 用二氧化矽凝膠作為充填劑之管柱用作分離床(c〇lumn bed) 的層析即可，可為任一樣式。該二氧化矽凝膠管柱層析可 具體列舉如：使溶劑以重力落下而流送之開管式層析、以 泵使溶劑流送之快速管柱層析、高速液體層析(HPLC)等。 5忒荨之中，尤以開管式層析及快速管柱層析於工業上容易 實施而較理想。 本第2-2步驟之二氧化矽凝膠管柱層析所用移動層宜 使用乙細或乙酸乙醋所構成之溶液（以下記為溶液a)與 正己烷、石油醚或正庚烷所構成之溶液（以下記為溶液B)之 10混合溶液。該混合液中相對於溶液A之溶液B的混合比可例 示如：相對於溶液A每1容量份，溶液3為2〜5〇容量份，且 宜為3〜10容量份。 #如此’將前述第2_i步驟所得萃取液供予二氧化石夕凝膠 管柱層析’即可回收含雌馬紛之分液。 15 前述於第2_2步驟所得之含雌騎之分液將供予以下 第2-3步驟。 第2-3击_ 20 從前述第2·2步騎得含雌,_之分液去除溶劑，對所 得殘留物進行再結晶化處理，回收析出之結晶（第^步驟）。 =從前述第2_2步驟所得含雌㈣之分液去除溶 d，可藉減壓濃縮等習知溶劑去除方法進行。 / .斤STre 口日日儿楚狂尸汀使用之溶劑僅^ 用於雌馬化即可，未受特觀制，但: 醇與水之混合液（乙醇水紐）、乙醆以旨、乙酸乙㈣ 42 200906315 之混合液以及乙醇與己烷之混合液。該等之中，較佳之溶 劑可列舉如乙酸乙酯與己烷之混合液及乙醇水溶液。再結 晶處理所使用之乙酸乙酯與己烷之混合液可具體例示如乙 酸乙酯：己烷之容量比為10:丨〜丨：10之混合液。此外，再 5結晶處理所使用之乙醇水溶液的乙醇濃度可列舉如3〇〜9〇 容量％，且宜為4〇〜7〇容量％。藉由使用前述濃度範圍之乙 酸乙酯與己烷之混合液或乙醇水溶液進行再結晶化，可獲 得更高純度之雌馬盼。 使其冷卻，即可折出雌馬酚之結 本第2-3步驟之再結晶化處理可依一般方法實施。舉例 H)來說’前述第2-2步驟所得含雌馬齡之分液去除溶劑而獲得 殘留物，加入再結晶用之溶劑並於加溫下使其溶解，之後 放率地進行雌馬⑫之結晶析出，亦可撥種雌馬⑪之結晶種。Column Chn>_g_y)m ship-like (four) column chromatography (7) ash C0lumn ch_t ()graphy, high-speed liquid chromatography (10) (6). Among the 15 j and so on, it is preferable to open tube chromatography and fast column chromatography for easy implementation in the health industry. " From the viewpoint of improving the degree of mystery of the female horse, it is preferable to use a mixed layer of hexane and scale for the moving layer (developing solvent) used in the silica gel column chromatography of the first step M. Here, the type of shouting used in the moving layer is not particularly limited, and examples thereof include dimethyl ketone, methyl ethyl ketone, and diethyl ether. These may be used singly or in combination of two or more. From the viewpoint of further improving the purification degree of the female horse, the above-mentioned shouting is preferably the same as that used in the mixed liquid of the above-mentioned first to fourth steps. The elution of the estrogen age caused by the cerium oxide gel column chromatography in the step M is preferably carried out by a gradient method, that is, 39 200906315, the same composition of the mixture of the burned and the bond used in the above steps 1-4. As the start = moving layer, and the ratio of the ether in the moving layer is gradually increased as before, the ratio is one, and the layer is separated from the impurity and dissolved. Therefore, it can escape the recovery of a fossil eve condensed = the column containing Ke dissolved in the female horse _ _ _ _ _ _ _ _. Then, by subtracting the conventional methods such as German and dry, the extraction from the μ5 step is used to remove the extraction _, which can be used to purify the high-purity solid-shaped carved horse. ★ 10 2-2. Purification of the second method 沬篦2-1 Step 骒 In the purification method of the second method, the first method is selected from the group consisting of acesulfame, alcohol, acetone, diwei, acetonitrile, As an extraction solvent, J 15 kinds of organic solvents or an aqueous organic solvent thereof in the group consisting of diethyl hydrazine and hydrazine are subjected to extraction treatment of 15 equol contents, and the resulting extract is concentrated (step 2). In the step 2-1, the extraction solvent may use at least a pure organic solvent selected from the group consisting of Q oxime ethyl ester, alcohol, acetone, dioxane, acetonitrile, diethyl ether and hydrazine, but From the standpoint of efficient extraction of equol - an aqueous organic solvent obtained by adding 20 to the organic solvent. Here, the alcohol used as the extraction solvent, such as methanol, ethanol, isopropanol or n-propanol, may be exemplified. The concentration of the organic solvent in the aqueous organic solvent may be, for example, 8 Torr to 99% by volume and preferably 90 to 97% by volume. By satisfying the pre-existing increase of the month], the range of the agricultural range can be 40 200906315 to obtain an extract with a high equol content. The extraction solvent used in the second step of the first step is preferably, for example, aqueous acetic acid. The treatment conditions for the extraction are also not particularly limited, and for example, it can be carried out by a dipping method such as cold soaking, 5 warm dipping, or the like. Specifically, the equol-containing material is added to the extraction solvent, and it is allowed to stand or stir at a normal temperature for 30 minutes or more (preferably at a normal temperature for 1 hour to 50 hours). The treatment conditions for the extraction treatment carried out in the second step are also not particularly limited. For example, it may be carried out by a dipping method such as cold soaking or warm dipping, a permeation method or the like. 10 After extraction as described above, equol is extracted into the extract, and the extract can be recovered by solid-liquid separation methods such as filtration and centrifugation. Next, the obtained extract was subjected to a concentration method such as concentration under reduced pressure to obtain an equol concentrate. The resulting equol concentrate will be supplied in the next step 2-2. 15 Step 2-2 The equol concentrate obtained in the above step 2-1 is supplied to a cerium oxide gel column chromatography to obtain a equol-containing liquid (step 2-2). In the second step 2-2, the equol concentrate obtained in the above step 2-1 can be directly supplied to the ceria gel column chromatography. 20, in the above step 2-1, when extracting with a water-soluble solvent containing alcohol, after concentrating the extract, it is preferable to add 5-10 times of ethyl acetate to the concentrate and mix with an equal amount of water. Then, the ethyl acetate was separated and concentrated, and subjected to column chromatography. The cerium oxide gel column chromatography in the second step 2 - 2 can be used as a separation column (c〇lumn bed) by using 41 200906315 with a ceria gel as a filling agent. Can be of any style. The cerium oxide gel column chromatography can be specifically exemplified by open-tube chromatography in which a solvent is dropped by gravity, rapid column chromatography by a pump to carry a solvent, and high-speed liquid chromatography (HPLC). Wait. Among them, open-tube chromatography and flash column chromatography are particularly easy to implement in the industry. The moving layer used in the column chromatography of the cerium oxide gel column of the second step 2-2 is preferably composed of a solution of ethylene or ethyl acetate (hereinafter referred to as solution a) and n-hexane, petroleum ether or n-heptane. A 10 mixed solution of the solution (hereinafter referred to as solution B). The mixing ratio of the solution B to the solution B of the solution A can be, for example, 2 parts by volume to 2 parts by volume, and preferably 3 to 10 parts by volume per part by volume of the solution A. #如' The extract obtained in the above step 2_i is supplied to the silica gel column column chromatography to recover the fraction containing the equine. 15 The above-mentioned split containing female ride obtained in the second step 2 will be supplied to the next step 2-3. Step 2-3 _ 20 The solvent is removed from the liquid containing the _, and the residue is recrystallized, and the precipitated crystal is recovered (the second step). = The solution of the liquid containing the female (4) obtained in the above step 2-2 is removed, and the solvent can be removed by a conventional solvent removal method such as concentration under reduced pressure. / 斤 ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST ST A mixture of ethylene (IV) 42 200906315 and a mixture of ethanol and hexane. Among these, preferred solvents include a mixture of ethyl acetate and hexane and an aqueous ethanol solution. The mixture of ethyl acetate and hexane used for the recrystallization treatment can be specifically exemplified by a mixture of, for example, ethyl acetate:hexane having a volume ratio of 10:丨~丨:10. Further, the ethanol concentration of the aqueous ethanol solution used for the recrystallization treatment may be, for example, 3 〇 to 9 容量 % by volume, and preferably 4 〇 to 7 〇 % by volume. By using the above-mentioned concentration range of a mixture of ethyl acetate and hexane or an aqueous solution of ethanol to recrystallize, a higher purity of estradiol can be obtained. The equol can be folded off by cooling it. The recrystallization treatment in the second to third steps can be carried out according to a general method. For example, H), the above-mentioned 2-2 step-containing liquid separation solution is used to remove the solvent to obtain a residue, and the solvent for recrystallization is added and dissolved under heating, and then the female horse is discharged 12 The crystals are precipitated, and the crystal species of the female horse 11 can also be planted.

。再者，為了順利且有 J依所使用溶劑之沸點及凝 可於溶劑之沸點溫度附近使 本發明提供一 —種含雌馬齡之大豆 換去材。& 其卒取物係分散於可可換中. Further, in order to be smooth and have a boiling point and a coagulation of the solvent to be used in the vicinity of the boiling point of the solvent, the present invention provides a soybean-containing soybean-replaced material. & its strokes are scattered in cocoa

與前述「 含雌馬酚 I含雌馬㉒之萃取物的製造方法 之大豆胚轴發酵物相同。 豆胚轴發酵物或 茲將本發明之食 之大立胚軸發酵物係 方法」一攔中所載之 43 200906315 含雌馬盼之大豆胚轴發酵物可 用在本發明之食品素材上， 發酵後之狀態使 理製成乾燥固態物狀，再使用於本===予乾燦處 5 10 15 20 ^含雌㈣之大豆胚㈣酵物之保存^^中。從 看，含雌馬狀大豆叫 的銳點來 固態狀。此外，經加熱乾燥處理大燥處理:成 酵物亦可依需要而供予粉末化卢王，』:大且胚軸發 含_之大豆，酵狀。It is the same as the soybean hypocotyl fermented product containing the equol I-containing extract of the female horse 22, and the bean hypocotyl fermented product or the method of the vegan hypocotyl fermented product of the present invention is blocked. 43 200906315 The soybean hypocotyl fermented product containing the female horse can be used on the food material of the present invention, and the state after fermentation is made into a dry solid state, and then used in the present === 10 15 20 ^ Preservation of the yeast (4) yeast containing the female (four) ^^. From the point of view, the sharp point containing the female horse-like soybeans is solid. In addition, the drying treatment is carried out by heat drying: the fermented material can also be supplied to the powdered king according to the need, ": large and hypocotyls containing soybeans, yeast.

—二 LI :==::物:萃取物並未特·， 所載之製造方法而獲得之^卒取物的製造方法」一欄 前述第戊法之製造方法 7之萃取物’且更宜為以 於本發明中，含雕馬^雄馬盼之萃取物。 以液狀直接㈣，但仍宜* h胚軸發酵物的萃取物可 本發明之食品素材中I:溶劑製成固態狀再使用。 物或其萃取物均可，卜技用含雌馬盼之大豆胚軸發酵 馬盼之大豆胚轴發酵物^養學上之觀點來看，宜為含雖 _可可塊（cacao 可可塊係將可可豆 驟而可獲得之食品原料發酵步驟、培炒步驟及磨碎步 發明之食品素材所用可破當為巧克力之原料來使用。本 可，並未特別受限。可塊僅需為可用作食品原料者即 44 200906315 、=本發明之食品素材中，含雌馬#之大豆胚軸發酵物 或其萃取物與可可塊之配合比並未特別受限，但從可具有 更良好之風味的觀點來看，可列舉如：相對於含雌馬盼之 5大絲軸發酵物或其萃取物(乾燥重量換算)觸重量份，可 可塊為10〜2000重量份，且宜為2〇〜_重量份，更宜為 20〜400重量份。特別是若於使用含雌馬齡之大豆胚轴發酵 物夺可歹J舉如.相對於含雌馬盼之大豆胚 重量換算_重量份，可可塊為·〜綱重量份，且 1〇 200〜900重量份，更宜為200〜4〇〇重量份。 … 此外，本發明之食品素材可僅由含雖馬盼之大豆 發酵物或其萃取物以及可可塊所構成，但除了該等成分以 夕卜’如後述，亦可含有其他配合成分。此二成分之配合比 ^對於本發明之食品素材總量，含雌馬盼之大豆胚轴發 酵物或其萃取物與可可塊之合計量宜佔40〜100重量％，且 較宜6〇〜95重量％，更宜7〇〜95重量％。以此種比例含有含雌 馬盼之大豆胚軸發酵物或其萃取物及可可塊可於具有良 好風味之同時，有效表現出基於含雌馬盼之大豆胚轴發^ 物的有用生理活性。 20佳意配合成分 本發明之食品素材除了（A)含雌馬_ 物或其萃取物及⑻可可塊㈣，科妨礙本發 限，亦可含有其他配合成分。 為 糖、海 可配合於本發明之食品素㈣以可列舉如砂 45 200906315 藻糖、乙醯績胺酸钾、木醣醇、嚴糖素(酿ai〇se)、赤藻糖 醇(erythritol)、阿斯巴甜、麥芽糖等甜味劑；可可脂、植物 性油脂等油脂·，大豆卵磷脂等乳化劑、香料；香料製劑; 牛奶；水等。 5 材之形狀 本發明之食品素材形狀並未特別受限可為粉末狀、 粒狀、片狀、平板狀等，可依所使用之食品種類而加以適 * 口又疋《可使本發明之食品素材的食感活用在食品中的 觀點來看，宜為粒狀或片狀，特別是每冰或每^平均為 10 30〜lOOOmg程度的粒狀或片狀。 的劁诰太沐 含雌馬紛之大豆胚軸發酵物或其萃取物、可可塊、 及^需其他配合成分以可使可可塊所含可可麟融之溫度 (且30〜50 C)條件下混人，接英入" 15 20 σ 卩固化_製出本發明之 食。素材。如此，可調製出可可塊 豆胚軸發酵物的本發明食品素材。 一馬盼之大 本=之食品素材可依需要供予破碎、 處理’以成形為所需形狀。此外，本 :粒專 於製造時简壓造㈣錢行_。 素材亦可 本發明之料食^讀㈣ 造原料或食品添加劑，而被配合於各二 明更k供-縣前述食品料之含 P，本發 該含雕馬盼之食品並未特別受限，舉t言，可列舉 46 200906315 如烘烤點心、冷凍點心等食品形態。 前述烘烤點心可列舉如方塊米餅（Arare)、御柿餅 (Okaki)等米菜類；鬆餅、威化餅等小麥煎餅類；軟餅 (biscuit);小甜餅(cookie);硬餅乾(cracker);派(pie);蛋糕； 5 多旱茲·’麵包等。此外，前述冷束點心可列舉如冰淇淋、 到冰、冰棒等。 前述含雌馬酚之食品中’可列舉烘烤點心作為較佳形 態之例。舉例來說，以大豆粉末作為原料而製得之烘烤點 心而言，因其除含有雌馬酚以外更綜合地含有源自大豆之 10有用成分，有用性高且可達到更佳之保健效果。 以大豆粉末作為原料而製得之烘烤點心的較佳例可列 舉如：不含麵粉且配合大豆粉末、蛋、奶油等而製得之烘 烤點心。此種小甜餅所使用之大豆粉末以供加熱處理等， 使脂肪加氧酶(lipoxygenase)失活或減弱化，且大豆味消失 15或減輕者為佳。舉例來說，將大豆粉末100重量份、蛋〇〜35 重量份、奶油10〜85重量份、所需形狀之本發明食品素材 0.1〜50重量份及適量其他配合成分混合於適量水中而奥 混合原料（生料），將其成形為預定形狀並以2〜8〇 、 80〜250°C程度烘培，即可製得前舰烤點心。乃、 2〇 就該含雌馬酚之食品所含本發明食品素材之八 而言，並未特別受限，可依本發明食品素材二己合比例 量、該含雌馬酚之食品的形態等加以適當設：酚含 說，可例示如：以該含雌馬紛之食品的原料總量:例來 發明之食品素材佔3〜30重量％(宜5〜20重量％，“，使本 文宜5〜8舌旦 47 200906315 %)之比例。此外，該含雌馬盼之食品所含雌馬酌·比例可例 示如：以該含雌馬酚之食品的原料總量計，使雌馬酚佔 0.002〜0.1重量％(宜0.004〜0.05重量％，更宜0.005〜0.03重量 %)的範圍。以此種比例含有本發明之食品素材，可使含雌 5 馬酚之食品保持良好風味，同時有效享受基於含雌馬酚之 大豆胚軸發酵物的有用生理活性。 除了本發明之食品素材以外，以預定量混合該食品之 其他原料，依該食品之種類適當供予成形、烘焙、冷卻等 步驟，即可製得該含雌馬酚之食品。 10 該含雌馬酚之食品含有含雌馬酚之大豆胚轴發酵物， 而含有以雌馬酚為始之各種有用生理活性物質，因此可展 現各種生理活性及藥理活性。故而該含雌馬酚之食品除了 作為一般食品之外，可作為特定保健用食品、營養輔助食 品、機能性食品、病患食品等來使用。含有本發明大豆胚 15 軸發酵物之食品特別是作為營養輔助食品甚為有用。 舉例而言，該含雌馬酚之食品在預防或改善更年期障 礙、骨質疏鬆症、前列腺肥大、代謝症候群等疾病或症狀、 減低血中膽固醇值、美白、改善挫瘡、整腸、改善肥胖、 利尿等上甚為有用。其中，該含雌馬酚之食品特別是在預 20 防或改善中高齡女性更年期不定之違和以及伴隨停經之症 狀(如骨質疏鬆症、更年期障礙等）上甚有用。 該含雌馬酌·之食品的每日平均攝取量可依該食品中之 雌馬酚含量、攝取者年齡及體重、攝取次數等而加以適當 設定。 48 200906315 4·备種形態之令兄 5 10 15 :本發明可提供含有含雄馬紛之大豆 或其卒取物之各_態食品。轉贿提供之具 態可列舉如飲料、補充品、奶油狀食品、甜點'點心^ 味料、㈣袋食品、食用加^品、揉合食品、蛋加碎 品、罐頭或罐裝食品、麵包、冷康點心、大豆加工食品: 米飯食品及湯品等。 °σ 發明所使用之含雌馬紛之大豆胚軸發酵“ 卒取物及本發明之食品形態詳述如下。 /、 本發明所使用之含雌馬 物係嫩㈣其萃取 酵物 酵物。 ，且έ有含雌馬酚之大豆胚軸發 t品形熊 含雌馬酚之大豆胚軸發 更年期障礙、骨質疏鬆症、予，或其萃取物在預防或改善 病或症狀、減低血中膦、腺肥大、代謝症候群等疾 ° ^醇值、盖A . 改善肥胖及利尿等用途上甚有、、改善挫瘡、整腸、 高齡女性之更年期不^ 、a用’特別是在預防或改善中 質疏鬆症、更年期障礙等^二U及伴隨停經之症狀(如骨 之各種形態食品亦可供用/途上甚有用。因此，本發明 性食品。即，本發明之含有乍為以則述用途為目的之機能 大丑胚轴發酵物的食品除了作 49 200906315 為一般食品之外，可作為特定保健用食品、營養輔助食品、 機能性食品、病患食品等來使用。本發明之含有大豆胚軸 發酵物之食品在作為營養輔助食品上特別有用。 5 10 15 20 兹就本發明所提供之具體食品形態加以具體說明。 ⑴飲料 本發明提供一種含有含雌馬紛之大豆胚軸發酵物或其 萃取物的飲料。 、 本發明之飲料種類並未特別受限，可列舉如.果十# 料；蔬果汁；氣泡水、可樂、果汁碳酸飲料、薑汁汽水（ ale)、蘋果汁(cider)及蘇打等之碳酸飲料；運動飲料等主 凉飲料水；咖啡、咖啡牛奶等之咖啡飲料；紅欠 μ月 、、’t、、綠茶、 烏龍茶等之茶飲料；可可亞；乳酸菌飲料；牛 果/ |牛 奶、酸乳飲料等之乳飲料；啤酒、燒酒、威士 Λ W、雞尾酒、 雨味燒酒等之酒精飲料等。 本發明飲料中之含雌馬酚之大豆胚軸發酵物或其4 ㈣含有比例並未特別受限，可依該大豆料發 萃取物中之雌馬酚含量、飲料種類、所期待之效 s /、 以適當設定。舉例來說可例示如：於飲料中，大-等來加 酵物或其萃取物(乾燥重量換算)佔G.G2〜15重量。轴發 番' Μ 〇/ I 且 0 · 1 〜1 里置/〇,更宜0.2〜0.5重量％)之比例。 本發明提供-種含有含雌馬盼之大豆胚轴發 萃取物之補充品。 或其 本發明之補充品種類並未特別受限，可列舉如耖膠 50 200906315 ㈣㈣、錠劑、粉劑等。該等之 膠。在軟膠及粉劑的情況下，宜以將其預定如軟 中之狀態來提供。 谷在膠囊 本發明之軟膠的較佳實施態様之一可例示如於 明勝、水及甘油之軟膠料添加含雌馬盼之大豆胚軸^酵 物或其萃取物者。除了前述成分之外，軟膠囊亦可含 有大丑油、Μ ' _磷月旨及_蘿蔔素等添加劑。 本發明補充品中之含雌馬紛之大豆胚軸發酵物或其萃 取物的含有關縣彳―，可依社豆轉發酵物或 其萃取物中之雌騎含量、補充品種類、所期待之效果等 而加以適當設定。舉例來說可例示如：於補充品中，大豆 胚軸發酵物或其萃取物(乾燥重量換算)佔2〜1〇〇重量％之 例。 (3)奶油（cream)狀含兄 15 20 本發明提供一種含有含雌馬盼之大豆胚軸發酵物或其 萃取物之奶油狀食品。 ' 本發明之奶油狀食品的種類並未特別受限，可列舉如 泡沫奶油、卡士達奶油、奶油、鮮奶油、咖啡奶精等。+ 本發明之奶油狀食品中之含雌馬紛之大豆胚轴發酵物 或其萃取物的含有比例並未特別受限，可依該大豆胚轴發 酵物或其萃取物中之雌馬紛含量、奶油狀食品種類、所期 待之效果等而加以適當設定。舉例來說可例示如：於奶由 狀食品中’大豆胚軸發酵物或其萃取物（乾燥重量換口 0.03〜5重量％(宜0.15~1重量〇/〇，更言〇 更且〇·3〜0.5重量之比例。 51 200906315 Μ)甜點（dessert} 萃取=供―種含有—〜酵物或其 本發明之甜點種類並未特別受限，可列舉如 凍、布丁及慕斯等。 優格、 本發明甜點中之含雌㈣之大豆_ 10 物的含有比例並未特別受限，可依該大趣轴取 萃取物中之雌㈣含量、甜點_、_待之欵或其 以適當设定。舉例來說可例示如：於甜點中 而加—II LI :==::: The extract is not specially prepared, and the method of manufacturing the product obtained by the method of manufacture is as described in the column For the purpose of the present invention, it comprises an extract of the eagle horse. The extract of the fermented product directly in the form of liquid (4), but still suitable for the hypocotyl can be used in the food material of the present invention, and the solvent is solidly used. The material or its extract can be used, and the technology uses the soybean hypocotyl fermentation of the horse-looking soybean, and the soybean hypocotyl fermented material of the horse is expected to be a cacao cocoa block. The food raw material fermentation step, the frying step and the grinding step of the food material of the invention can be used as a raw material for chocolate. The present invention is not particularly limited. The raw material of the food material is 44 200906315, = the food material of the present invention, the ratio of the soybean hypocotyl fermented product containing the female horse # or its extract to the cocoa mass is not particularly limited, but can have a better flavor. In view of the above, for example, the cocoa mass is 10 to 2000 parts by weight, and preferably 2 〇 to _, relative to the weight of the 5 large wire shaft fermented product or the extract thereof (in terms of dry weight). The parts by weight are more preferably 20 to 400 parts by weight, especially if the soybean hypocotyl fermented product containing the estrogen age is used, as compared with the weight of the soybean embryo containing the female horse, the cocoa is converted. The block is a part by weight and is 1 〇 200 to 900 parts by weight, more preferably 2 00〜4〇〇重量份。 In addition, the food material of the present invention may be composed only of the soybean fermented product containing Mawang, or its extract and cocoa mass, but in addition to the ingredients, as will be described later, Other compounding ingredients may be contained. The compounding ratio of the two components is 40 to 100% by weight of the total amount of the food material of the present invention, and the total amount of the soybean hypocotyl fermented product or the extract thereof and the cocoa mass containing the female horse is preferably 40 to 100% by weight. More preferably, it is preferably from 6 to 95% by weight, more preferably from 7 to 95% by weight. In this proportion, the soybean hypocotyl fermented product containing oryrogan or its extract and cocoa mass can be effectively used while having a good flavor. It exhibits useful physiological activity based on the soybean hypocotyl hairs containing the espresso. 20 Optimistic ingredients The food material of the present invention is obstructed in addition to (A) female horses or their extracts and (8) cocoa masses (4) The present invention can also contain other compounding ingredients. For the sugar and sea, the food substance (4) which can be blended with the present invention can be exemplified by, for example, sand 45 200906315, sucrose, acetaminophen potassium, xylitol, and sucrose. Ai〇se), erythritol, aspartame Sweeteners such as maltose; fats and oils such as cocoa butter, vegetable oils and fats, emulsifiers and flavors such as soybean lecithin; perfume preparations; milk, water, etc. 5 Shape of the material The shape of the food material of the present invention is not particularly limited. In the form of a powder, a granule, a sheet, a plate, or the like, it may be appropriately selected according to the type of the food to be used, and it is preferable that the food material of the present invention can be used in foods. Granular or flaked, especially in the form of granules or flakes on an average of 10 30~100 mg per ice or per gram. The 劁诰太沐 contains the fermented soybean hypocotyls or its extract, cocoa mass, And other ingredients are required to mix the temperature of the cocoa block containing cocoa (and 30~50 C), and then enter the " 15 20 σ 卩 curing _ to produce the food of the present invention. material. Thus, the food material of the present invention which can ferment the cocoa pea pulp hypocotyls can be prepared. A large amount of food material can be crushed and processed as needed to form into a desired shape. In addition, this: Granules are specially designed for manufacturing (4) money line _. The material may also be used as a raw material or a food additive in the present invention, and is blended with each of the above-mentioned food materials of the second and further k-prefectures, and the food containing the carved horse is not particularly limited. For example, 46 200906315 such as baked snacks, frozen snacks and other food forms. Examples of the above-mentioned baked snacks include rice dishes such as Arare and Okaki; wheat pancakes such as muffins and wafers; biscuits; cookies; and hard biscuits. (cracker); pie (pie); cake; 5 more than Zhazi · 'bread and so on. Further, the aforementioned cold-boiled snacks may, for example, be ice cream, ice, popsicle or the like. In the above-mentioned equol-containing food, a baked snack can be exemplified as a preferred form. For example, a baking point made of soybean powder as a raw material has a usefulness and a better health effect because it contains a useful ingredient derived from soybean in addition to equol. A preferred example of the baked snack prepared by using soy powder as a raw material may be, for example, a baked snack prepared without flour and blended with soy powder, egg, cream or the like. The soy powder used in such a cookie is subjected to heat treatment or the like to inactivate or weaken the lipoxygenase, and the soy taste is lost or reduced. For example, 100 parts by weight of soybean powder, egg gluten to 35 parts by weight, cream 10 to 85 parts by weight, 0.1 to 50 parts by weight of the food material of the present invention in a desired shape, and an appropriate amount of other compounding ingredients are mixed in an appropriate amount of water and mixed. The raw material (raw material) is shaped into a predetermined shape and baked at a temperature of 2 to 8 Torr and 80 to 250 ° C to obtain a front ship baked snack.乃 〇 〇 〇 〇 〇 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 含 食品 食品 食品 食品 食品 食品 食品 食品 食品 食品 食品And the appropriate setting: the phenolic content can be exemplified by, for example, the total amount of raw materials containing the female horse food: the food material invented by the example accounts for 3 to 30% by weight (preferably 5 to 20% by weight, ", to make this article The ratio of 5 to 8 tongues 47 200906315 %). In addition, the proportion of the females in the food containing the espresso can be exemplified, such as: taking the total amount of raw materials of the equol-containing food, making the female The phenol is in the range of 0.002 to 0.1% by weight (preferably 0.004 to 0.05% by weight, more preferably 0.005 to 0.03% by weight). The food material of the present invention is contained in such a ratio, and the food containing the equol is maintained in a good flavor. At the same time, the useful physiological activity of the equol-containing soybean hypocotyl fermented product is effectively enjoyed. In addition to the food material of the present invention, other raw materials of the food are mixed in a predetermined amount, and appropriately shaped, baked, and cooled according to the type of the food. The equol-containing food can be obtained by the steps 10 The equol-containing food contains equol-containing soybean hypocotyl fermented product, and contains various useful physiologically active substances starting from equol, thereby exhibiting various physiological activities and pharmacological activities. In addition to being a general food, the food of the phenol can be used as a specific health food, a nutritional supplement, a functional food, a patient food, etc. The food containing the soybean shaft 15-axis ferment of the present invention is particularly useful as a nutritional supplement. For example, the equol-containing food prevents or improves diseases or symptoms such as menopausal disorders, osteoporosis, prostatic hypertrophy, metabolic syndrome, reduces blood cholesterol levels, whitens, improves contusion, and improves intestinal tract It is useful to improve obesity, diuresis, etc. Among them, the equol-containing foods are particularly effective in preventing or improving the menopause of middle-aged women and the symptoms associated with menopause (such as osteoporosis, menopausal disorders, etc.). Useful for the daily average of the food containing the female horse, depending on the equol content of the food, the year of the ingestor Age, weight, number of intakes, etc. are appropriately set. 48 200906315 4·Make the form of the brother 5 10 15 : The present invention can provide various foods containing the soybeans containing the male horses or their pawns. Examples of offerings include beverages, supplements, creamy foods, desserts, snacks, foods, foods, foods, egg and crumbs, canned or canned foods, bread, and cold. Kang Dim Sum, Soybean Processed Food: Rice Food, Soup, etc. °σ Soybean Hypocotyl Fermentation Containing Equine in the Invention "The sample of the stroke and the food form of the present invention are detailed below. /, the estrogen-containing system used in the present invention is (4) the extract of the fermented yeast. And the soybean hypocotyl containing equol, the t-shaped bear equol, the soybean hypocotyl, the menopausal disorder, osteoporosis, pre-, or its extract in preventing or improving the disease or symptom, reducing blood Phosphine, glandular hypertrophy, metabolic syndrome, etc. ° Alcohol value, cover A. Improve the use of obesity and diuretic, and improve the menopause of the contusion, the whole intestine, the elderly women, and the use of 'especially in prevention or Improve the symptoms of neutropenia, menopausal disorders, etc., and the symptoms associated with menopause (such as bones of various forms of food are also available / useful on the way. Therefore, the present invention food, that is, the present invention contains 乍In addition to general food, it can be used as a specific health food, a nutritional supplement, a functional food, a patient food, etc. The food of the present invention contains soybeans. The food of the hypocotyl fermented product is particularly useful as a nutritional supplement. 5 10 15 20 The specific food form provided by the present invention is specifically described. (1) Beverage The present invention provides a female-containing horse. A beverage of the soybean hypocotyl ferment or its extract. The type of the beverage of the present invention is not particularly limited, and examples thereof include a fruit juice, a vegetable juice, a bubble water, a cola, a juice carbonated beverage, and a ginger soda. (ale), cider and soda carbonated beverages; main beverages such as sports drinks; coffee beverages such as coffee, coffee and milk; tea drinks such as red moon, 't, green tea, oolong tea, etc. ; cocoa; lactic acid bacteria beverage; bovine fruit / | milk, yogurt drink, etc.; beer, shochu, Visa W, cocktail, rain shochu, etc. Alcoholic beverages, etc. The soybean hypocotyl fermented product or its 4 (tetra) content ratio is not particularly limited, and may be appropriately set depending on the equol content of the soybean extract, the type of the beverage, and the expected effect s / . For example, in a beverage, a large-equivalently added yeast or an extract thereof (calculated by dry weight) accounts for G.G 2 to 15 weight. Axis hair is ' Μ 〇 / I and 0 · 1 〜 1 is set / 〇, More preferably, the ratio is 0.2 to 0.5% by weight. The present invention provides a supplement containing an extract of soybean hypocotyls containing equuma. The supplemental variety of the present invention or the like is not particularly limited, and examples thereof include silicone rubber 50 200906315 (four) (four), tablets, powders and the like. These gums. In the case of soft gels and powders, it is preferred to provide them in a state of being soft. Gum in Capsules One of the preferred embodiments of the soft gel of the present invention is exemplified by the addition of a soy embryonic extract containing equuma or an extract thereof to a soft gum of Mingsheng, water and glycerin. In addition to the aforementioned ingredients, the soft capsules may contain additives such as large ugly oil, Μ ' _ Phosphorus and _ radish. In the supplement of the present invention, the soybean-containing hypocotyl fermented product or the extract thereof contains the relevant county 彳 ―, and the content of the female rider, the supplement variety, and the expected The effect and the like are appropriately set. For example, in the supplement, the soybean hypocotyl fermentate or the extract thereof (in terms of dry weight) accounts for 2 to 1% by weight. (3) Cream-like brothers 15 20 The present invention provides a creamy food containing a fermented soybean hypocotyl or an extract thereof. The type of the creamy food of the present invention is not particularly limited, and examples thereof include foam cream, custard cream, cream, whipped cream, coffee creamer and the like. + The content ratio of the soybean-containing hypocotyl fermented product or the extract thereof in the creamy food of the present invention is not particularly limited, and may be according to the content of the equine in the soybean hypocotyl ferment or its extract. The type of the creamy food, the desired effect, and the like are appropriately set. For example, it can be exemplified as: 'soybean hypocotyl fermentate or its extract in milk-like foods (dry weight change 0.03~5 wt% (preferably 0.15~1 weight 〇/〇, more 〇 〇 〇 〇 〇 51 to 0.5 weight ratio. 51 200906315 Μ) dessert (dessert) extraction = for the species - the yeast or the type of the dessert of the present invention is not particularly limited, and examples thereof include frozen, pudding, and mousse. The content of the female (4) soybean _ 10 in the dessert of the present invention is not particularly limited, and the content of the female (four) in the extract, the dessert _, the _ 欵 欵 or the appropriate For example, it can be exemplified as:

酵物或其萃取物(乾燥重量換算)佔0.03〜5重量。㈣由發 重量％，更宜0.3〜0.5重量％)的比例。 里〇(宜0.15H ⑸點心 本發明提供—種含有含雌馬酚之 萃取物之點心。 物或其 15 20 果 本發明之點心不論西式點心或日式點心的差里’ 烘烤點心及蒸煮點心等各色點心、。具體來說，林日包含 心可例示如:糖果、麥芽糖、焦糖、普澤餅(Pret吟:之點 =香糖、、巧克力、小甜餅、豆菓子、鬆餅、起司硬^、、 膠狀點心（gummy)、煎餅、洲濱餅（Suhama)、千菓子、、，己、 傻頭、嚴餅、雪藏蛋糕(p〇und cake)、蘋果派餡、零’囊 點心及御柿餅等。該等點心之中，較佳實施態樣之(:， 舉如含有大豆粉之烘烤點心。可列 本發明點心中之含雌馬酚之大豆胚軸發酵物或其^ 物之含有tt例並未制受限，可依該大豆胚|^酵物^ 52 200906315 萃取物中之雌馬酚含量、點心種類、所期待之效果等而加 以適當設定。舉例來說可例示如：於點心中，大豆胚軸發 酵物或其萃取物（乾燥重量換算）佔〇_〇1〜99重量％(宜 〇.〇4〜38重量％’更宜〇.〇8〜18重量。/。)之比例。 5 ί一6)調呋料 本發明提供一種含有含雖馬紛之大豆胜轴發酵物或其 萃取物之調味料。 本發明之調味料種類並未特別受限’舉例來說可列舉 如：烤肉醬、蒲燒醬、花枝珍咮之調味料、番茄醬' 醬油、 10沾醬、芝麻醬、零嘴調味料及醬油等。 本發明調味料中之含雌馬齡·之大豆胚轴發酵物或其萃 取物之含有比例並未特別受限，玎依该大豆胚軸發酵物或 其萃取物中之雌馬酚含量、調味料種類、所期待之效果等 而加以適當設定。舉例來說可例示如：於調味料中，大豆 15胚軸發酵物或其萃取物（乾燥重量換算)佔〇·〇4〜4〇重量％(宜 〇.2〜8重量％，更宜0.4〜4重量％)之比例。 12)殺菌袋舍兄 胚軸發酵物或其 本發明提供一種含有含雌馬酚之大豆 萃取物之殺菌袋食品（retort pouch)。 限’可列舉如咖 本發明之殺菌袋食品種類並未特別受 哩、燉牛肉、徵菜(stew)、肉#、白醬等之醬素；中華并、 牛丼、親子丼等丼素；麻婆赠素、炊飯料之調味料材 料等。 本發明殺g袋食品中之含雌祕之大豆胚轴發酵物或 53 200906315 其萃取物的含有比例並未特別^ π㈣以㈣ 物或其萃取物中之雌馬紛含量、殺g袋食品_、_ 之效果等而加以適當設定。裂办，七^ 舉例來說可例示如：於殺菌袋 食品中，大絲軸發酵物或其萃取物（乾燥重量換 5 0.003〜4重量％(宜0.01〜0.8重暑。/ „ 里里、 ΐ®〇/〇,更宜〇·03〜〇4重量％)之比 例。 (8) 食用加工肉品 本發明提供-種含有含雌馬紛之大豆胚轴發酵物或其 萃取物之食用加工肉品。 ' 10 本發明食用加工肉品之種類並未特別受限，可列舉 如：香腸(乾式、半乾式、破洛尼亞、法蘭克福、維也納 煙燦）、肝[培根、燻肩肉（shoulder bac〇n)、火腿、漢保 肉、肉丸子等。 t 本發明之食用加工肉品中之含雌馬盼之大豆胚袖發酵 μ物或其萃取物之含有比例並未特別受限，可依該大豆 發酵物或其萃取物中之雌馬盼含量、食用加工肉品種類、 所期待之效果等而加以適當設定。舉例來說可例示如.於 食肉加工食品中，大豆胚轴發酵物或其萃取物(乾燥拖 算)佔0.02〜0.4重量％(宜〇__重量％，更宜g 2邊4 、 之比例。 〇) (9) 揉合舍品 本發明提供-種含有含雌馬盼之大豆胚轴發酵 萃取物之揉合食品。 本發明之揉合食品種類並未特別受限可列舉如魚板 54 200906315 及竹輪等。 本發明揉合食品中之含雕馬紛之大豆胚 萃取物之含有比例並未特別受限，可依該大豆胚二: 5 10 15 20 或其萃取物中之雌馬盼含量、揉合食品種類、所酵物 果等而加以適當M。舉例來說可例示如：於掉^致 大豆胚軸發酵物或其萃取物(乾燥重量換算^ “。中’ %⑽重量％，更宜0.3〜G5重量％)之比例。·〜重量 (10)蛋加工舍品 本發明提供一種含有含雌馬盼之大 萃取物之蛋加工食品。 發酵物或其 本發明之蛋加工食品種類並未特別 湯調味煎蛋、厚煎蛋、蛋包(_det) '炒衫。可列舉如高 本發明蛋加工食品中之含雌㈣之大豆 其萃取物之含有比例並未特別受限，可依車發酵物或 物或其萃取物中之雌馬酚含量、蛋力工且胚輛發酵 之效果等而加以適當設定。舉例來品:類、所期待 二’大豆轉發酵物料萃取她燥二:蛋加工食 重篁(宜重量％，更宜 換罐〇.03〜5 .重量0/❶)之比例。 本發明提供-種含有含雌㈣之大— 物之罐頭或轉食品。 酵物或其萃取 本發明之罐頭或罐裝食品種 如:掛福及桃子等之水果罐頭‘=受限’可列舉 果番等之_;湯品等 海產罐項； 晋汁#之罐頭等。 55 200906315 5 10 15 20 本發明之罐頭或罐裝食品中的含雌馬紛之大豆胚轴發 酵物或其卒取物之含有比例並未特别受限，可依該大豆胚 軸發酵物或其萃取物中之雌㈣含量、罐頭或罐裝食品種 類、所期狀效果㈣加以適#設定。舉例來說可例示如. 於罐頭或罐裝食品中，大豆胚軸發酵物或其萃取物(乾燥重 量換算）佔0.03〜5重量％(宜(uq重量％，更宜〇3〜〇5重量 %)之比例。 Π2)麵包 本發明提供-種含有含雌馬盼之大豆胚轴發酵物或其 萃取物之麵包。 〃 本發明之麵包種類並未特別受限，可列舉如：吐 •J 、 法國麵包、調理麵包、哈密瓜麵包、紅豆麵包、奶油麵包 果醬麵包、捲麵包、牛角麵包(croissant)等。 本發明麵包中之含雌馬酚之大豆胚軸發酵物或其萃取 物之含有比例並未特別受限，可依該大豆胚軸發酵物咬其 萃取物中之雌馬酚含量、麵包種類、所期待之效果等而力 以適當設定。舉例來說可例示如：於麵包中，大豆肢轴發 酵物或其萃取物（乾燥重量換算）佔0.01〜5〇重量。^ 0.05~0.8重量％，更宜〇.1~0.3重量％)之比例。 (13)洽凍點心 本發明提供一種含有含雌馬酚之大豆胚軸發酵物或发 萃取物的冷凍點心。 ' 本發明之冷凍點心種類並未特別受限，可列舉如水 淋、雪霜（sherbet)等。 56 200906315 本發明冷凍點心中之含雌馬酚之大豆胚軸發酵 萃取物的含有比例並未特別受限 ，可依該大豆胚輛或其 或其萃取物中之雌馬紛含量、冷滚點心種類、所期待^物 果卓而加以適當設定。舉例來說可例示如：於冷;東點,、、六 5大五胚軸發酵物或其萃取物（乾燥重量換算）佔0.03\5 = %(宜0·1〜1重量％，更宜0.3〜0.5重量％)之比例。 量 本發明提供一種含有含雌馬盼之大豆胚轴發酵物或其 萃取物的大豆加工食品。 1〇 本發明大豆加工食品之種類並未特別受限，可列舉如 丑腐、炸油豆腐、雁擬(Ganm〇d〇ki)、湯葉（Yuba)、納豆、 豆乳等。 本發明大豆加工食品中之含雌馬酚之大豆胚軸發酵物 或其萃取物之含有比例並未特別受限，可依該大豆胚軸發 15酵物或其萃取物中之雌馬酚含量、大豆加工食品種類、所 期待之效果等加以適當設定。舉例來說可例示如：於大豆 加工食品中，大豆胚軸發酵物或其萃取物（乾燥重量換 佔0.03〜0.5重量％(宜o.w重量％，更宜〇 3〜〇 5重量％)之比 例。 20 Π 5)米飯食品 本發明提供-種含有含雌馬紛之大豆胚轴發酵物或其 萃取物的米飯食品。 本發明米飯食品之種類並未特別限制’可列舉如炒 飯、Pilaf、粥、栗子糯米飯、白飯、紅豆飯、五目飯等。 57 200906315 本發明米飯食品中之含雌馬紛之大豆胚軸發酵物或其 萃取物的含有比例並未特別受限，可依該大豆胚軸發酵物 或其萃取物中之雌馬酚含量、米飯食品種類、所期待之效 果等加以適當設定。舉例來說可例示如：於米飯食σ中 5大豆胚轴發酵物或其萃取物（乾燥重量換算）佔〇〇1 25重 量％(宜〇_〇2〜0.5重量°/〇，更宜〇·〇4〜0.25重量〇/〇)之比例 Π6)湯品 本發明提供-種含有含雌馬紛之大豆胚轴發酵物或其 萃取物之湯品。 1〇 2發明之湯品種類並未特別受限，可列舉如玉米奶油 湯、清肉湯(c__0 soup)、菜麵濃湯(mi_r〇ne 、 洋蔥湯及濃湯等。 本發明湯品中含雌馬紛之大豆胚軸發酵物或其萃取物 的含有比例並未特別受限，可依該大豆胚轴發酵物或其萃 15取物中之雌馬紛含量、湯品種類、所期待之效果等加以適 當設定。舉例來說可例示如：於湯品中，大豆胚轴發錄物 或其卒取物（乾燥重量換算）佔0 02〜15重 % ’更宣0.2〜0.5重量％)之比例。 · 【實施例] 2〇 —參考例、實施例等詳細說明本發明於下，但本發 明不侷限於該等例示。 製造含雌祕之大ί胚軸發酵物 如表1所示組成，混合粉末狀大豆胚軸、精㈣及水， 出大豆胚轴溶液(原料）。於該大豆胚轴溶液5ml中植入 58 200906315 乳球菌2〇-92菌株阳腹βρ•刪㈣），於厭氧 37 C進行96小時靜置培養。培養後將所得發酵液(、下以 則〇〇C、】分鐘之條件加熱殺菌後，以8(Tc之條件後) 5 15 燥處理’更以均質儀進行粉末化處理，而獲得 丁乾 豆胚軸發酵物。 狀之大 —炫將培養96小時後之培養液巾的生菌數及pH、粉 大旦胚轴發酵物之取得量、及粉末狀大豆胚軸發酵物中之 :馬紛濃度示於表卜從此結果確認，使用雌騎產生菌2 叔末狀大五胚軸發酵可高效率地纽雖馬紛。The yeast or its extract (calculated by dry weight) accounts for 0.03 to 5 weight. (4) The ratio of % by weight, more preferably 0.3 to 0.5% by weight. 〇((0.15H(5) Dim Sum) The present invention provides a snack containing an extract containing equol. Or a snack of the present invention, whether it is a Western-style dim sum or a Japanese dim sum, a baked dessert and cooking Dim sum and other snacks. In particular, Lin Ri contains the following examples: candy, maltose, caramel, Puzze cake (Pret吟: point = chewing gum, chocolate, cookies, bean fruit, muffins) , cheese hard ^, gelatin (gummy), pancakes, Suhama, thousand fruit, ,, own, silly, cake, p〇und cake, apple pie, zero a snack, a persimmon, etc. Among these snacks, a preferred embodiment (:, for example, a baked snack containing soy flour, may be listed as an equol-containing soybean hypocotyl fermentate in the dessert of the present invention or The tt example of the substance is not limited, and may be appropriately set according to the equol content of the extract of the soybean embryo, the amount of the snack, the desired effect, etc., for example, For example: in the snack, soybean hypocotyl ferment or its extract (dry weight change ) 〇 〇 〜 〜 〜 〜 〜 〜 〜 〜 〇 〇 〇 〇 〇 〇 〇 〇 〇 〜 〜 〜 〜 〜 〜 〜 ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) ) The seasoning containing the soybean stalk fermented product or its extract. The seasoning type of the present invention is not particularly limited. For example, barbecue sauce, poached sauce, and squid seasonings can be cited. , tomato sauce, soy sauce, 10 sauce, sesame paste, snack seasoning, soy sauce, etc. The proportion of the soybean hypocotyl fermented product or its extract contained in the seasoning of the present invention is not particularly limited, and is converted. The soy hypocotyl fermented product or the extract thereof, the equol content, the type of the seasoning, the desired effect, and the like are appropriately set. For example, in the seasoning, the soybean 15 hypocotyl ferment or The extract (dry weight conversion) accounts for 4~4〇% by weight of 〇·〇4~4重量%, preferably 0.4~4% by weight. 12) Sterilization bag The present invention provides a retort pouch containing equol-containing soybean extract. The limitation may be exemplified by the fact that the sterilized bag food type of the invention is not particularly affected by glutinous rice, stewed beef, stewed vegetables (stew), meat #, white sauce, etc.; Chinese glutinous rice, burdock, parent-child glutinous rice, etc.; Ma Po, a seasoning material for glutinous rice, etc. The soybean-containing hypocotyl fermented product in the g-bag food of the present invention or 53 200906315 The content of the extract is not particularly π (4) to (4) or its extraction The content of the female horse in the product, the effect of killing the food _, _, etc., is appropriately set. The splitting, for example, can be exemplified, for example, in the sterilized bag food, the large shaft ferment or its extract (dry weight for 5 0.003~4% by weight (preferably 0.01~0.8 heavy heat. / „ 里 里 ΐ 〇 〇 〇 03 03 03 03 03 03 03 03 03 03 03 03 03 03 03 03 03 ( ( ( ( ( ( ( ( ( ( 食用 食用 食用 或其 或其 或其 或其 或其 或其 或其 或其 或其 或其 或其 或其Edible processed meat of the extract. ' 10 The types of edible processed meats of the present invention are not particularly limited, and examples thereof include: sausages (dry, semi-dry, broken Lonia, Frankfurt, Vienna), liver [Bacon , shoulder bac〇n, ham, hanbao meat, meatballs, etc. t The ratio of the content of the soybean embryo sleeve fermentation or the extract thereof contained in the edible processed meat of the present invention It is not particularly limited, and may be appropriately set depending on the content of the estrogen in the soybean fermented product or its extract, the type of edible processed meat, the desired effect, etc., for example, in the processed meat processing food. , soybean hypocotyl ferment or its extract (drying calculation) accounted for 0.02~0.4% by weight (preferably __% by weight, more preferably g 2 side 4, the ratio. 〇) (9) 舍合舍品 the invention Providing a kneading food containing a soybean hypocotyl fermentation extract containing equuma. The type of the food to be blended is not particularly limited, and examples thereof include fish plate 54 200906315 and bamboo wheel, etc. The content ratio of the soybean embryo extract contained in the kneaded food of the present invention is not particularly limited, and the soybean embryo can be used according to the soybean embryo. 2: 5 10 15 20 or the content of the female horse in the extract, the type of the food, the fruit, etc., and the appropriate M. For example, it can be exemplified as: the soybean hypocotyl fermented product or its extraction The ratio of the dry weight conversion ^". % '% (10)% by weight, more preferably 0.3 to G5% by weight). - Weight (10) Egg processing product The present invention provides an egg processed food containing a large extract containing ergot. The fermented product or the egg processed food of the present invention is not particularly flavored, fried omelet, thick omelet, egg wrap (_det) 'fried shirt. For example, the content of the extract containing the female (4) in the egg processed food of the present invention is not particularly limited, and the equol content of the fermented product or the extract thereof or the egg can be used. The effect of the embryo fermentation is appropriately set. For example: class, expected 2' soybean transfer fermentation material extraction her dry two: egg processing food heavy 篁 (% by weight, more suitable for cans. 03~5. Weight 0 / ❶) ratio. The present invention provides a can or a food product containing a large product containing a female (four). Yeast or its extraction The canned or canned food of the present invention, such as: canned fruit such as hanging blessings and peaches, ‘=restricted' can be enumerated as fruit, etc.; soups and other seafood cans; canned juices such as cans. 55 200906315 5 10 15 20 The content of the soybean-containing hypocotyl fermented product or its pawn in the canned or canned food of the present invention is not particularly limited, and may be based on the soybean hypocotyl fermentate or The content of the female (four) in the extract, the type of canned or canned food, and the effect of the period (4) should be set. For example, in a canned or canned food, the soybean hypocotyl ferment or its extract (in terms of dry weight) accounts for 0.03 to 5% by weight (preferably (uq% by weight, more preferably 〇3 to 〇5 by weight). The ratio of %) Π 2) Bread The present invention provides a bread comprising a fermented soybean hypocotyl or an extract thereof. The type of the bread of the present invention is not particularly limited, and examples thereof include spitting J, French bread, conditioning bread, cantaloupe bread, red bean bread, buttered bread, jam bread, rolled bread, and croissant. The content ratio of the equol-containing soybean hypocotyl fermented product or the extract thereof in the bread of the present invention is not particularly limited, and the equol content, the bread type, and the type of the bread may be bitten according to the soybean hypocotyl fermentate. The expected effect and the like are set as appropriate. For example, it can be exemplified that in the bread, the soybean limb fermented product or the extract thereof (in terms of dry weight) accounts for 0.01 to 5 ounces by weight. ^ 0.05~0.8% by weight, more preferably 1.1~0.3% by weight). (13) The frozen confectionery The present invention provides a frozen confectionery containing the equol-containing soybean hypocotyl fermentate or hair extract. The type of the frozen dessert of the present invention is not particularly limited, and examples thereof include water shower, sherbet, and the like. 56 200906315 The content ratio of the equol-containing soybean hypocotyl fermentation extract in the frozen dessert of the present invention is not particularly limited, and may be according to the content of the equine horse in the soybean embryo or its extract, cold-rolled snack The type and the expected result are appropriately set. For example, it can be exemplified as: cold; east point,,, six five large hypocotyl fermented matter or its extract (dry weight conversion) accounted for 0.03\5 = % (preferably 0·1~1% by weight, more preferably A ratio of 0.3 to 0.5% by weight). The present invention provides a soybean processed food containing a fermented soybean hypocotyl or an extract thereof. 1〇 The type of the soybean processed food of the present invention is not particularly limited, and examples thereof include ugly rot, fried tofu, Ganm〇d〇ki, Yuba, natto, and soymilk. The content ratio of the equol-containing soybean hypocotyl fermented product or the extract thereof in the soybean processed food of the present invention is not particularly limited, and the equol content of the 15 protein or the extract thereof may be determined according to the soybean hypocotyl, The type of soybean processed food, the expected effect, and the like are appropriately set. For example, it can be exemplified, for example, in the soybean processed food, the proportion of the soybean hypocotyl ferment or its extract (dry weight is changed by 0.03 to 0.5% by weight (it is preferably ow% by weight, more preferably 〇3 to 5% by weight). 20 Π 5) Rice Food The present invention provides a rice food containing a fermented soybean hypocotyl or an extract thereof. The type of the rice food of the present invention is not particularly limited, and examples thereof include fried rice, Pilaf, porridge, chestnut glutinous rice, rice, red bean rice, and five-headed rice. 57 200906315 The content ratio of the soybean-containing hypocotyl fermented product or the extract thereof in the rice food of the present invention is not particularly limited, and may be based on the equol content of the soybean hypocotyl fermentate or its extract, The type of rice food, the expected effect, etc. are appropriately set. For example, it can be exemplified as: 5 rice hypocotyl fermented product or its extract (in dry weight conversion) in rice rice σ accounted for 1.255% by weight ((〇 〇 〜 2~0.5 weight ° / 〇, more preferably 〇 〇4~0.25重量〇/〇) ratio Π6) Soup The present invention provides a soup containing a fermented soybean hypocotyl or a extract thereof. The type of the soup of the invention is not particularly limited, and examples thereof include corn cream soup, clear broth (c__0 soup), vegetable noodle soup (mi_r〇ne, onion soup, and soup). The soup of the present invention contains females. The content ratio of the soybean hypocotyl fermented product or the extract thereof is not particularly limited, and may be based on the content of the female horse in the soybean hypocotyl ferment or its extract, the soup variety, and the expected effect. For example, it can be exemplified, for example, in the soup, the ratio of the soybean hypocotyls or its pawn (in terms of dry weight) to 0 02 to 15% by weight, more than 0.2 to 0.5% by weight. [Embodiment] The present invention is described in detail below with reference to examples, examples, and the like, but the present invention is not limited to the examples. The production of the hyphae fermented product containing the female secret is as shown in Table 1, and the powdered soybean hypocotyls, the fine (four) and the water are mixed, and the soybean hypocotyl solution (raw material) is produced. Into 5 ml of the soybean hypocotyl solution, 58 200906315 Lactococcus 2〇-92 strain Yang ββρ• deleting (4)) was implanted in anaerobic 37 C for 96 hours. After culturing, the obtained fermentation broth is heated and sterilized under the conditions of 〇〇C, □ minutes, and then dried under a condition of 8 (after Tc conditions) 5 15 to be pulverized by a homogenizer to obtain diced beans. Hypocotyl fermented material. The large number of spleen---the number of bacteria and pH of the culture liquid towel after 96 hours of cultivation, the amount of powdered macro-denier hypocotyl fermented material, and the powdery soybean hypocotyl fermented product: The concentration is shown in the table. From this result, it is confirmed that the use of the female riding bacteria 2 undisturbed big five hypocotyl fermentation can be highly efficient.

結果，左側數值表示ί 会施(^F^TTiFy·^^· mg(2 wt· 表中以.表示未測定，括弧内量ϋ之各異相比率 參考例1-4冑造含雄騎之大豆胚轴發酵物 於含有粉末狀大豆胚〇 t大m y < 里/。及L-精胺酸0.1重量％ 之大旦胚軸…ml中植入乳球 BP-臟號），⑽條件下以听靜 行發酵處理。培養後 口養仏’以進 分鐘之條件加所付發酵液(培養_政」 ‘、'、权围後’謂C之條件進行賴處理，更 59 20 200906315 以均質儀進行粉末化處理，而獲得粉末狀之大豆胚軸發酵 物。 分析作為原料使用之粉末狀大豆胚軸（表2及3中記為 發酵則）及所得粉末狀大豆胚軸發酵物（表2及3中記為發酵 後）=含有成分。大豆異黃_類之分析結果示於表2，營養 ^刀之刀析結果示於表3。從此結果亦可確認，藉乳球菌 2〇 92菌株使大豆胚軸發酵，可製造含高含量雌馬紛之大豆 發酵物。此外’確認使用棉子糖(raffin〇se)及水蘇糖 10 ::::等募::在發酵前後含量為相咐 酚，亦可產生 變為鳥胺酸1此Γ已確認精胺酸會因發酵處理而轉 菌㈣菌株進知於大豆胚轴添加精胺酸並以乳球 鳥胺酸。 酵處理，不僅可產生雌馬 200906315 表2 大豆異黃酮類 每 100g 成分項目 發酵前 發酵後 雌馬酚 N.D 632.Omg 大豆苷 566.4mg 29.7mg 丙二醯大豆苷 124.9mg N.D. 乙醯大豆苷 364.8mg 25.4mg 木質素黃酮 7.lmg 24_4mg 二氫木質素黃酮 N.D. 49.4mg 染料木苷 117.lmg 3.2mg 丙二醯染料木苷 35. lmg N.D. 乙醯染料木苷 146. lmg 3.7mg 金雀素黃酮 0.9mg 22.5mg 二氫金雀素黃酮 N.D. 112.Omg 黃豆苷 331.7mg 53.6mg 丙二醯黃豆苷 65.0mg N.D. 乙醯黃豆苷 169.2mg 34.8mg 黃豆素黃酮 19.lmg 292.3mg 二氫黃豆素黃酮 N.D. 8.2mg 總異黃酮 1942.Omg 1291.2mg N.D.表示未測出。 表3 每 100g 營養成分 成分項目 發酵前 發酵後 水分 3.2g 蛋白質 38.lg 脂質 13.0g 灰分 4.3g 糖質 3〇.9g 食物纖維 l〇.5g 熱量· 414kcal 蔗糖 7.95g 棉子糖 l_37g 水蘇糖 9.04g 反式脂肪酸 N.D. 磷脂質 3_33g (硬脂醯、油酷、卵填脂） 游離精胺酸 88 lmg 游離鳥胺酸 N.D. 皂素醇（sapogenol)A N.D. 皂素醇B N.D. 大豆皂苷 3.6g 6.2g 38.3g 14.5g 4.〇g 26.8g l〇.2g 411kcl 7.42g 1.34g 8.38g N.D. 2.92g 8 8)·). S Ώ 6 D D 8 Γ ο · · . ,2·°Ν N 1 N.D.表示未測出。 61 製造含雌馬酚 除了使用與前述參考例K3$5足胚轴發酵物 以外’以與前述參考例M相@ @之7批粉末狀大豆胚軸 發酵物（參考例1-5〜1-11)。分析戶條件製造粉末狀大豆胚軸 黃鲷組成。將杜果+热表二所得大豆胚軸發酵物所含異 無法實現之組成的異黃酮。、、、紛3量Ν’含有習知技術 10 參考例1-5 座 參考例1-7 參考例1-8 參考例1-9 參考例1-10 參考例1-11 6-51 mg (62.2 yft%} 6.25 rng _i6L3wt%) 5.38 mg (48.9 wt%^ 6.43 mg (63.4 wt.%-> 6.05 mg (64.2 wt.%') 6.11 mg (65.6 wt.%^ 6.3 mg (60.9 wt.%) 0- 48 mg-iilwt%; 1- 18 mgQ^Zwt%) 0.61 mg 〇5TiJ~ 0.37 mg 0-49 mg 組成 0.53 mg mm 2.71 mg ---(5.1 wt.%) (25.9 wt.%) 0.35 mg 3,12 mg __ (3.4 wt.%) (30.6 wt.%) 1.45 mg 3.00 mg (13.2 wt.%) (27.2 wt.%) 0.48 mg 2.62 mg —(4.7 wt.%) (25.8 wt.%) 0.30mg 2.57 mg (3.2 wt·%) (27.3 wt.%) 0.10 mg 2.74 mg (1-lwt.%) (29.4 wt.%) 0.37 mg 3.19mg (3.6 wt.%) (30.8 wt.%) 重量％) if'j I-11 ° υ-Hy m〇 TT^Z---- __(60.9 wt%) f47w〇/x 0.37 mg X?，上段‘數值(3.6wt.%) 下段之數值顯示相對於大&之各異黃酮含I 之各異黃酮比率（重量％)。丑胚軸發酵物所含異黃酮總量（100 急_考試驗例丄致敏原之確認試驗 已知大豆胚軸含有Gym4、Gm30K、Gm28K、7S球蛋 白πΰχ(β-組大豆球蛋白（p_c〇ngiyCinin))、油體蛋白、騰蛋白 15酶抑制因子等致敏原。於此，藉下述試驗來判定前述參考 例1-1所製造含雌馬酚之大豆胚軸發酵物中是否存有致敏 原。 首先’將參考例1-1所得含雌馬酚之大豆胚轴發酵物適 62 200906315 量添加於萃取緩衝液(Tds HC1 ρΗ 7·5，含1M EDTA，含有 適量蛋白質分解酶素抑制劑），充分授拌後萃取水溶性成 刀接著’以過濾去除固態成分，獲得萃取液。使用 社製之蛋白質分析系統檢測如此製得之萃取液所含總蛋白 5質’此外’，亦以西方墨點法檢剛所得萃取液所含主要致敏 原（Gym4、Gm30K、Gm28K、7S球蛋白_、油體蛋白胰 蛋白酶抑制因子）。此外’作為對照，使用大豆子葉粉末及 大豆胚軸粉末取代大豆胚軸發酵物，以相同方法進行總蛋 白質及致敏原之檢測。 1〇 彡將結果示於第1〜3圖。第1圖顯示總蛋白質之檢測結 果，第2圖顯示Gym4、Gm3〇K、及如胤之檢測結果，第 3圖則顯示7S球蛋白mix、油體蛋白及姨蛋白酶抑制因子之 檢測結果。 從此一結果可確認，含雌馬酚之大豆胚軸發酵物中， 15大丑或大丑胚軸所含主要致敏原已減低。 實施例1 :敦逢金^雌馬酚之萃敌物 實-毯例1-1~~製造含雌馬酚之茬取物 將以與參考例U相同條件製造之粉末狀含雌馬盼之 大旦胚軸發酵物1g添加於表5所示萃取溶 劑20ml，以室溫振 20蘯120分鐘。之後，以離心分離去除殘渣（以下記為殘渣… 回收萃取液（以下記為萃取壯1)。接著，使用基發哭 (evaporator)使所得萃取液1-1乾涸後 ，力口入5ml乙醇，以室 溫振盡财鐘。之後，以離心分離去除殘逢（以下記為殘潰 )回收萃取液（以下記為萃取液1-2)。使用蒸發器使所得 63 200906315 萃取液1-2乾涸後，獲得固態狀萃取物。 此外，對殘渣1再次添加相同組成之萃取溶劑20ml，以 室溫振盪120分鐘。之後，以離心分離去除殘渣，回收萃取 液（以下，記為萃取液2-1)。使用蒸發器使所得萃取液2-1乾 5 涸後，添加5ml乙醇，以室溫振盪10分鐘。之後以離心分離 去除殘渣，回收萃取液（以下記為萃取液2-2)。使用蒸發器 使所得萃取液2-2乾涸，獲得固態狀萃取物。 表5 萃取溶劑之 表記 萃取溶劑之組成 25Et 由25容量％乙醇及75容量％純水構成之乙醇水溶液 50Et 由50容量％乙醇及50容量％純水構成之乙醇水溶液 75Et 由75容量％乙醇及25容量％純水構成之乙醇水溶液 lOOEt 100容量％乙醇 將萃取液1-1及1-2、萃取液2-1及2-2以及殘渣2供予薄 10 層層析（TLC)分析（移動相在檢測雌馬酚時使用己烷：氯 仿：乙酸乙酯=1 : 1 : 1(容量比），檢測皂苷時使用乙酸乙 酯：丙醇：氯仿：甲醇:0.9%KC1=25:25:25:10:9(容量比）），測 定皂苷含量與雌馬酚含量。此外，測定從萃取液1-2所得固 態狀萃取物之重量及其所含雌馬酚濃度。結果示於第4〜7 15 圖及表8。第4圖顯示以TLC分析萃取液1-2及2-2中之雌馬酚 含量的結果；第5圖顯示以TLC分析萃取液1-2及2-2中之皂 苷含量的結果；第8圖顯示以TLC分析萃取液1-2及殘渣2中 之雌馬酚含量的結果；第7圖顯示以TLC分析萃取液1-2及殘 渣2中之皂苷含量的結果。 20 由此結果得知，若以乙醇萃取含雌馬酚之大豆胚軸發 酵物，將含有多量油分，且雌馬酌之回收率降低。另一方 64 200906315 面，則確認：若以乙醇水溶液萃取含雌馬酚之大豆胚軸發 酵物，將抑制油分之混入，而以高回收率獲得雌馬酚。特 ，若以乙醇濃度為75容量％之乙醇水溶液，雌馬盼回 收率將顯著提高。此外，得知75容量％乙醇萃取可將脂肪 分之萃取抑制至最小限度。 此外，由於殘渔2中檢測出高濃度皂苷，已確認可將萃 取液1-1之溶劑除去物供予乙醇之萃取處理，藉此可不致減 少峨馬酚量而去除皂苷。 10As a result, the value on the left side indicates that ί will apply (^F^TTiFy·^^·mg (2 wt· in the table to indicate that it has not been measured, and the ratio of the amount of 括 in the brackets refers to Example 1-4. The hypocotyl fermented product is implanted in a large-denier hypocotyl ... ml containing powdered soybean embryos t large my < and L-arginine 0.1% by weight in the large-density hypocotyls ... ml, under conditions (10) Listen to the static fermentation process. After the cultivation, the broth is added to the conditions of the fermentation broth (cultivation_government), ', ', and the right after the right C, and the condition of C is further treated. 59 20 200906315 Powdered treatment was carried out to obtain a powdery soybean hypocotyl ferment. The powdery soybean hypocotyls used as raw materials (denoted as fermentation in Tables 2 and 3) and the obtained powdery soybean hypocotyl fermentate were analyzed (Table 2 and The results of the analysis are shown in Table 2, and the results of the analysis of the nutrient knife are shown in Table 3. From this result, it was also confirmed that the Lactococcus ssp. 2〇92 strain was used. Soybean hypocotyl fermentation, can produce high-yield soy fermented soybeans. In addition, 'confirm the use of raffinose (raffin〇se ) and stachyose 10 :::: et al.:: before and after fermentation, the content is ruthenium phenol, and it can also be changed to ornithine. This Γ has confirmed that arginine will be transferred by fermentation (4) Adding arginine to soybean hypocotyls and treating them with lactobionine. Fermentation treatment can not only produce female horses 200906315. Table 2 Soy isoflavones per 100g ingredients. Fermentation of equol after fermentation 632.Omg daidzin 566.4mg 29.7mg propylene disaccharide daidin 124.9mg ND acetoin 364.8mg 25.4mg lignin flavonoids 7.lmg 24_4mg dihydro lignin flavonoids ND 49.4mg genist lignin 117.lmg 3.2mg propane bismuth glucoside 35. lmg ND acetyl glucoside 146. lmg 3.7mg minocycline flavonoid 0.9mg 22.5mg dihydro galactoflavonoid ND 112.Omg daidzin 331.7mg 53.6mg propylene diterpenoid glycoside 65.0mg ND acetaminoglycan 169.2mg 34.8 Mg Soybean flavonoids 19.1mg 292.3mg Dihydrodasin flavonoids ND 8.2mg Total isoflavones 1942.Omg 1291.2mg ND means not detected. Table 3 Per 100g nutrient composition project Fermentation before fermentation, water 3.2g Protein 38.lg Lipid 13.0g Ash 4.3g Glycogen 3〇.9g Food fiber l〇.5g Heat· 414kcal Sucrose 7.95g Raffinose l_37g Stachyose 9.04g Trans fatty acid ND Phospholipid 3_33g (stearin, oily, egg fat) Free arginine 88 lmg free ornithine ND sapogenol A ND saponin B ND soy saponin 3.6g 6.2g 38.3g 14.5g 4.〇g 26.8gl〇.2g 411kcl 7.42g 1.34g 8.38g ND 2.92g 8 8)·). S Ώ 6 DD 8 Γ ο · · . , 2·°Ν N 1 ND means not measured. 61 Manufacture of equol containing 7 batches of powdered soybean hypocotyl fermentate in addition to the above reference example K3$5 full hypocotyl fermentate with the aforementioned reference example M @ Reference Example 1-5~1-11 ). Analyze household conditions to produce powdered soybean hypocotyls. The isoflavones of the soybean hypocotyl fermented product obtained from Duguo + Hot Table 2 have different compositions. 、、、、3量Ν' contains conventional techniques 10 Reference Example 1-5 Block Reference Example 1-7 Reference Example 1-8 Reference Example 1-9 Reference Example 1-10 Reference Example 1-11 6-51 mg (62.2 Yft%} 6.25 rng _i6L3wt%) 5.38 mg (48.9 wt%^ 6.43 mg (63.4 wt.%-> 6.05 mg (64.2 wt.%') 6.11 mg (65.6 wt.%^ 6.3 mg (60.9 wt.%)) 0- 48 mg-iilwt%; 1- 18 mgQ^Zwt%) 0.61 mg 〇5TiJ~ 0.37 mg 0-49 mg Composition 0.53 mg mm 2.71 mg ---(5.1 wt.%) (25.9 wt.%) 0.35 mg 3,12 mg __ (3.4 wt.%) (30.6 wt.%) 1.45 mg 3.00 mg (13.2 wt.%) (27.2 wt.%) 0.48 mg 2.62 mg — (4.7 wt.%) (25.8 wt.%) 0.30 mg 2.57 mg (3.2 wt.%) (27.3 wt.%) 0.10 mg 2.74 mg (1-lwt.%) (29.4 wt.%) 0.37 mg 3.19 mg (3.6 wt.%) (30.8 wt.%) Weight %) if'j I-11 ° υ-Hy m〇TT^Z---- __(60.9 wt%) f47w〇/x 0.37 mg X?, the upper part 'value (3.6wt.%) The value of the lower part shows relative The isoflavone ratio of each of the isoflavones in Yida & (% by weight). The total amount of isoflavones contained in the ugly hypocotyl fermented product (100 急 _ test case 丄 allergen confirmation test known soybean hypocotyl Contains Gym4, Gm30K, Gm28K, 7S globulin πΰχ (β- A group of allergens such as glycoglobin (p_c〇ngiyCinin), oil body protein, and tensin 15 enzyme inhibitor. Here, the following test is used to determine the equol-containing soybean embryo produced in the above Reference Example 1-1. Whether the allergen exists in the shaft ferment. Firstly, the amount of the equol-containing soybean hypocotyl fermentate obtained in Reference Example 1-1 is added to the extraction buffer (Tds HC1 ρΗ 7·5, containing 1 M EDTA, Containing an appropriate amount of proteolytic enzyme inhibitor), fully mixing and extracting water-soluble knives and then removing the solid components by filtration to obtain an extract. The total protein contained in the extract thus obtained is detected by a protein analysis system manufactured by the company. The quality of 'external' is also detected by Western blotting method. The main allergens (Gym4, Gm30K, Gm28K, 7S globulin_, oil body protein trypsin inhibitor) are obtained from the extract. Further, as a control, the soybean cotyledon powder and the soybean hypocotyl powder were used in place of the soybean hypocotyl fermentate, and the detection of the total protein and the allergen was carried out in the same manner. 1〇 彡 The results are shown in Figures 1 to 3. Fig. 1 shows the results of detection of total protein, Fig. 2 shows the results of detection of Gym4, Gm3〇K, and Rugao, and Fig. 3 shows the results of detection of 7S globulin mix, oil body protein and chymotrypsin inhibitor. From this result, it was confirmed that the main allergen contained in the 15 ugly or large ugly hypocotyls of the equol-containing soybean hypocotyls had been reduced. Example 1: Dunfengjin^Erythramine extracts - blankets 1-1~~ The preparation of equol-containing extracts will be made in the same condition as the reference example U. 1 g of the large-density hypocotyl fermented product was added to 20 ml of the extraction solvent shown in Table 5, and the mixture was shaken at room temperature for 20 蘯 120 minutes. After that, the residue was removed by centrifugation (hereinafter referred to as residue... The extract was recovered (hereinafter referred to as extracting 1). Then, the obtained extract 1-1 was dried using an evaporator, and then 5 ml of ethanol was added thereto. The bell is shaken at room temperature. After that, the extract (hereinafter referred to as the residue) is recovered by centrifugation (hereinafter referred to as extract 1-2). The obtained 63 200906315 extract 1-2 is dried using an evaporator. Then, 20 ml of an extraction solvent of the same composition was added to the residue 1 and the mixture was shaken at room temperature for 120 minutes. Thereafter, the residue was removed by centrifugation, and the extract was recovered (hereinafter, referred to as extract 2-1) After the obtained extract 2-1 was dried over 5 Torr using an evaporator, 5 ml of ethanol was added thereto, and the mixture was shaken at room temperature for 10 minutes, and then the residue was removed by centrifugation, and the extract (hereinafter referred to as extract 2-2) was collected. The obtained extract 2-2 was dried by an evaporator to obtain a solid extract. Table 5 The composition of the extraction solvent The composition of the extraction solvent 25Et The aqueous ethanol solution consisting of 25 vol% ethanol and 75 vol% pure water 50Et from 50 vol% ethanol Ethanol aqueous solution of 50% by volume of pure water 75Et Ethanol aqueous solution of 75 % by volume of ethanol and 25 % by volume of pure water 100Et 100% by volume of ethanol Extracts 1-1 and 1-2, extracts 2-1 and 2-2 And the residue 2 was subjected to thin layer chromatography (TLC) analysis (the mobile phase used hexane: chloroform: ethyl acetate = 1: 1 : 1 (capacity ratio) in the detection of equol, and ethyl acetate was used for the detection of saponin. :propanol: chloroform: methanol: 0.9% KC1 = 25:25:25:10:9 (capacity ratio)), the saponin content and the equol content were measured. Further, the solid extract obtained from the extract 1-2 was measured. The weight and the concentration of equol contained therein. The results are shown in Figures 4 to 7 15 and Table 8. Figure 4 shows the results of analyzing the equol content of extracts 1-2 and 2-2 by TLC; Figure 5 shows the results of analyzing the saponin content in extracts 1-2 and 2-2 by TLC; Figure 8 shows the results of analyzing the equol content in extracts 1-2 and residue 2 by TLC; Figure 7 shows The result of analyzing the saponin content in the extracts 1-2 and the residue 2 by TLC. 20 As a result, it was found that if the equol-containing soybean hypocotyl fermentate was extracted with ethanol, it would contain A large amount of oil, and the recovery rate of the female horse is reduced. On the other side, 64 200906315, it is confirmed that if the equol-containing soybean hypocotyl fermented product is extracted with an aqueous solution of ethanol, the mixing of the oil is inhibited, and the female is obtained with high recovery. In the case of an aqueous ethanol solution having an ethanol concentration of 75 vol%, the recovery rate of the equol is significantly increased. In addition, it is known that 75 vol% ethanol extraction can suppress the extraction of fat fraction to a minimum. A high concentration of saponin was detected in the fish 2, and it was confirmed that the solvent removal of the extract 1-1 can be supplied to the extraction treatment of ethanol, whereby the saponin can be removed without reducing the amount of humin. 10

而算出之固態狀萃取物中之濃度比率。 丛第I方法製造含雌尾酚之萃取物And calculate the concentration ratio in the solid extract. Method for producing an extract containing cerulein by the method of the first method

除了使用與前述參考例1-3不同批之粉末狀大豆胚軸 is ，已與前述參考例丨_3相同之條件製造粉末狀含雌馬酚 之大足胚轴發酵物。 將该粉末狀含雌馬酚之大豆胚軸發酵物3〇g添加至7〇 今里％乙醇水溶液（於水3〇ml加入乙醇至1〇〇ml)4卯容量％ 乙醇水溶液（於水l〇ml加入乙醇至1〇〇ml)15〇ml，以室溫攪 2〇半8】、時。之後，過濾去除殘渣後，更以與萃取溶劑相同組 成之乙醇水溶液約20ml洗淨殘潰，回收滤液及洗淨液而獲 得萃取液。 接著，減壓浪縮所得萃取液而獲得固態狀萃取物。 65 200906315 測定所得固態狀萃取物之重量及其所含之雌馬酚、木 質素黃酮、二氫木質素黃綱、金雀素黃酮、二氫金雀素黃 酮、黃豆素黃酮、二氫黃豆素黃酮之濃度。茲將結果示於 表7及8。 表7 70容量％乙醇水溶液之萃取結果 經使用之含EQL大豆 回收之固態狀萃取物 重量 供萃取之量 (g) 30 乾燥重量 (mg) 8000 EQL EQL含量 (mg) EQL濃度 (重量％) EQL含量 (mg) EQL濃度 (重量％> EQL回收 率(％) EQL濃縮 倍率 161.40 0.54 158.98 1.98 98.5 3.7 DZN DZN含量 (mg) DZN濃度 (重量％) DZN含量 (mg) DZN濃度 (重量％) DZN回收 率(％) DZN濃縮 倍率(％) 6.00 0.02 0.00 0.00 0.0 0.0 DHD DHD含量 (mg) DHD濃度 (重量°/。） DHD含量 (mg) DHD濃度 (重量％) DHD回收 率(％) DHD濃縮 倍率(％) 9.00 0.03 0.09 0.00 0.00 0.00 GNT GNT含量 (mg) GNT濃度 (重量％) GNT含量 (mg) GNT濃度 (重量％) GNT回收 率(％) GNT濃縮 倍率(％) 6.90 0.02 0.31 ' 0.00 4.5 0.2 DHG DHG含量 (mg) DHG濃度 (重量％) DHG含量 (mg) DHG濃度 (重量％) DHG回收 率(％) DHG濃縮 倍率(％) 32.7 0.11 6.96， 0.09 21.3 0.5 GLY GLY含量 (mg) GLY濃度 (重量％) GLY含量 (mg) GLY濃度 (重量％) GLY回收 率(％) GLY濃縮 倍率(％) 62.70 0.21 33.17 0.41 52.9 2.0 DHG DHG含量 (mg) DHG濃度 (重量％) DHG含量 (mg) DHG濃度 (童量％) DHG回收 率(％) DHG濃縮 倍率(％) 0.9 0.00 0.00 0.00 0.00 0.00 表中，EQL為雌馬酚；DZN為木質素黃酮；DHD為二氫木質素黃酮；GNT為 金雀素黃酮；DHG為二氫金雀素黃酮；GLY為黃豆素黃酮；DHG為二氫黃豆 素黃酮。A powdery equol-containing large-foot hypocotyl fermentate was produced under the same conditions as the above-mentioned Reference Example 丨3 except that the powdery soybean hypocotyl is different from the above-mentioned Reference Example 1-3. Adding 3 〇g of the equol-containing soybean hypocotyl fermented product to 7 〇 % % % ethanol solution (adding ethanol to 1 〇〇 ml in 3 〇 ml of water) 4 卯 capacity % ethanol aqueous solution (in water l〇 Add 100 ml of ethanol to 1 〇〇 ml) 15 〇 ml, stir at room temperature for 2 〇 half 8]. Thereafter, the residue was removed by filtration, and washed with about 20 ml of an aqueous ethanol solution of the same composition as the extraction solvent, and the filtrate and the washing liquid were collected to obtain an extract. Next, the obtained extract was reduced under reduced pressure to obtain a solid extract. 65 200906315 Determine the weight of the obtained solid extract and its equol, lignin flavone, dihydrolignin xyloid, ginseng flavonoids, dihydrogen flavonoids, daidzein, dihydrodasin The concentration of flavonoids. The results are shown in Tables 7 and 8. Table 7 Extraction Results of 70% by Volume Ethanol Aqueous Solution Weight of Solid Extract Extracted with EQL Soybean Used for Extraction (g) 30 Dry Weight (mg) 8000 EQL EQL Content (mg) EQL Concentration (% by Weight) EQL Content (mg) EQL concentration (% by weight) EQL recovery (%) EQL concentration ratio 161.40 0.54 158.98 1.98 98.5 3.7 DZN DZN content (mg) DZN concentration (% by weight) DZN content (mg) DZN concentration (% by weight) DZN Recovery rate (%) DZN concentration ratio (%) 6.00 0.02 0.00 0.00 0.0 0.0 DHD DHD content (mg) DHD concentration (weight ° /.) DHD content (mg) DHD concentration (% by weight) DHD recovery (%) DHD concentration Magnification (%) 9.00 0.03 0.09 0.00 0.00 0.00 GNT GNT content (mg) GNT concentration (% by weight) GNT content (mg) GNT concentration (% by weight) GNT recovery (%) GNT concentration ratio (%) 6.90 0.02 0.31 ' 0.00 4.5 0.2 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) DHG concentration (% by weight) DHG recovery (%) DHG concentration ratio (%) 32.7 0.11 6.96, 0.09 21.3 0.5 GLY GLY content (mg) GLY concentration (% by weight) GLY content (mg) GLY concentration (% by weight) GLY back Yield (%) GLY concentration ratio (%) 62.70 0.21 33.17 0.41 52.9 2.0 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) DHG concentration (child%) DHG recovery (%) DHG concentration ratio (%) 0.9 0.00 0.00 0.00 0.00 0.00 In the table, EQL is equol; DZN is lignin flavone; DHD is dihydro lignin flavone; GNT is triadin flavonoid; DHG is dihydro gen flavonoid; GLY is Soybean flavonoids; DHG is dihydrodasin flavonoids.

各異黃明含量（mg)表示，經使用之含EQL大豆胚軸發酵物（30g)或回收之萃取物 10 (8000mg)所含各異黃洞之總重量，各異黃酮濃度（重量。/。)顯示經使用之含EQL 大豆胚軸發酵物或經回收之萃取物所含各異黃酮比例。 此外，濃縮倍率係指，令含EQL之大豆胚軸·^酵物所含各成分濃度為1而算出 之固態狀萃取物中之各成分濃度的比率。 66 200906315 表8 90容量％乙醇水溶液之萃取結果 5 經使用之含EQL大豆 回收之半固態狀萃取物 重量 供萃取之量 (g) 30 乾燥重量 (mg) 4100 EQL EQL含量 濃度 EQL含量 EQL濃度 EQL® 收 EQUIPS (mg) (重量％) (mg) (重量％) 率(％) 倍率 161.40 0.54 158.13 3.89 98.0 7.2 DZN DZN含量 DZN濃度 DZN含量 DZN濃度 DZN回收 DZN濃縮 (mg) (重量％) (mg) (重量％) 率(％) 倍率(％) 6.00 0.02 0.00 0.00 0.0 0.0 DHD DHD含量 DHD濃度 DHD含量 DHD濃度 DHD回收 DHD濃縮 (mg) (重量％) (mg) (重量％) 率(％) 倍率(％) 9.00 Ί 0.03 0.12 0.00 1.3 0.1 GNT GNT含量 GNT濃度 GNT含量 GNT濃度 GNT回收 GNT濃縮 (mg) (重量％) (mg) (重量％) 率(％) 倍率(％) 6.90 0.02 0.34 0.01 4.9 0.4 DHG DHG含量 DHG濃度 DHG含量 DHG濃度 DHG回收 DHG濃縮 (mg) (重量％) (mg) (重量％) 率(％) 倍率(％) 32.7 0.11 6.89 0.17 21.1 1.4 GLY GLY含量 GLY濃度 GLY含量 GLY濃度 GLY回收 GLY濃縮 (mg) (重量％) (mg) (重量 率(％) 倍率(％) 62.70 0.21 32.64 0.80 52.1 3.8 DHG DHG含量 DHG濃度 DHG含量 dug濃度 DHG回收 DHG濃縮 (mg) (重量％) (mg) (重量％) 率(％) 倍率(％) 0.9 0.00 0.00 0.00 0.00 0.00 - ' -;J5 ， 'X- ZN金表異回指之 D氫8)各或係中 •，二(m含物率物 盼為量所酵倍取 含g)發缩萃 rDHsom軸濃狀 句；黃10胚，態 Q-iq異(4豆外固 E黃各物大此之 Β5 為雀示黃收，各 為 度 濃 分 成 各 質D]酵量含 木·，發重。所 氫5轴^(例物 f胚U比酵 為素豆J酮發H:tis 5GLU量所之的 ，之重物L度 ί?.ΐΕΟ濃 GN黃經使 •，氫R經 同二g示 黃為(30表 素HG物%) 素。取以 雀網萃E t黃之含 為素收^ 一且回用 出 算 而 從此結果得知，若以乙醇水溶液萃取含雌馬酚之大豆 10胚軸發酵物，除可以高回收率獲得雌馬酚外，亦可獲得黃 丑素黃酮。另一方面，其他異黃酮在萃取物中濃度極低。 以第I方法製造含雌馬6^萃取物 除了使用與前述參考例1-3不同批之粉末狀大豆胚軸 卜以與前述參考例1-3相同之條件製造粉末狀含雌馬酚之 15大豆胚軸發酵物。 於邊粉末狀含雌馬酚之大豆胚軸發酵物5〇中加入純 67 200906315 水7ml混合後，更加入正己烷250ml並以室溫攪拌7小時。過 濾回收不溶物，更以60t進行7小時乾燥，獲得含雌馬酚之 大豆胚軸發酵物的脫脂粉末46.6g。 將前述脫脂粉末20g添加至80容量％乙醇水溶液（於水 5 中加入乙醇至100ml)或90容量％乙醇水溶液(於水1〇ml 中加入乙醇至l〇〇ml)l〇〇ml中，以室溫攪拌14小時。之後， 藉過濾去除殘渣後，更以與萃取溶劑相同組成之乙醇水溶 液約l〇ml洗淨殘渣’回收濾液及洗淨液而獲得萃取液。 接著’以60。(：乾燥所得萃取液6小時，獲得固態狀萃取 10 物。 測疋所得固態狀萃取物之重量及其所含雕馬驗、木質 素只綱、二氳木質素黃酮、金雀素黃酮、二氫金雀素黃酮、 黃豆素黃_、二氫黃豆素黃酮之濃度。結果示於表9及10。 200906315 表9 80容量％乙醇水溶液之萃取結果 經使用之含EQL大豆胚軸發 酵物的脫脂粉末 回收之固態狀萃取物 重量 供萃取之量 fe) 20 乾燥重量 (mg) 4000 EQL EQL含量 (mg) EQL濃度 (重量％) EQL含量 (mg) HJL濃度 (重量％) EQL回收 率(％) EQL濃縮 倍率 107.60 0.54 114.89 2.88 106.8 5.3 DZN DZN含量 (mg) DZN濃度 (重量％) DZN含量 (mg) DZN濃度 (重量％) DZN回收 率(％) DZN濃縮 倍率(％) 4.00 0.02 0.00 0.00 0.0 0.0 DHD DHD含量 (ms) DHD濃度 (重量％) DHD含量 (mg) DHD濃度 (重量％) DHD回收 率(％). DHD濃縮 倍率(％) 6.00 0.03 0.10 0.00 1.7 0.1 GNT GNT含量 (mg) GNT濃度 (重量％) GNT含量 (mg) GNT濃度 (重量％) GNT回收 率(％) GNT濃縮 倍率(％) 4.60 0.02 0.28 0.01 0.01 0.4 DHG DHG含量 (mg) DHG濃度 (重量％) DHG含量 (mg) DHG濃度 (重量％) DHG回收 率(％) DHG濃縮 倍率(％) 32.7 0.11 6.96 0.13 21.3 0.9 GLY GLY含量 (mg) GLY濃度 (重量％) GLY含量 (mg) GLY濃度 (重量％) GLY回收 率(％) GLY濃縮 倍率(％) 41.80 0.21 24.03 0.60 57.5 2.9 DHG DHG含量 (mg) DHG濃度 (重量％) DHG含量 (mg) DHG濃度 (重量％) DHG回收 率(％) DHG濃縮 倍率(％) 0.6 0.00 0.00 0.00 0.00 0.00 表中，EQL為雌馬酚；DZN為木質素黃酮；DHD為二氫木質素黃酮；GNT為 金雀素黃酮；DHG為二氫金雀素黃酮；GLY為黃豆素黃酮；DHG為二氫黃豆 素黃明。 5 各異黃酮含量（mg)表示，經使用之含EQL大豆胚軸發酵物的脫脂粉末（20g)或回 收之萃取物（4000mg)所含各異黃酮之總重量，各異黃酮濃度（重量％)表示經使用 之含EQL大豆胚轴發酵物或回收之萃取物所含各異黃酿1比例》 此外，濃縮倍率係指，令含EQL之大豆胚軸發酵物所含各成分濃度為1而算出 之固態狀萃取物中之各成分濃度的比率。 69 200906315 表10 90容量％乙醇水溶液之萃取結果 經使用之含EQL大豆胚軸發 酵物的脫脂粉末 回收之固態狀萃取物 重量 供萃取之量 (g) 20 乾燥重量 (mg) 2200 EQL EQL含量 (mg) EQL濃度 (重量％) EQL含量 (mg) EQL濃度 (重量％) EQL回收 率(％) EQL濃縮 倍率 107.60 0.54 106.88 4.81 99.3 8.9 DZN DZN含量 (mg) DZN濃度 (重量％) DZN含量 (mg) DZN濃度 (重量％) DZN回收 率(％) DZN濃縮 倍率(％) 4.00 0.02 0.00 0.00 0.0 0.0 DHD DHD含量 DHD濃度(重量 %) DHD含量 (mg) DHD濃度 (重量％) DHD回收 率(％) DHD濃縮 倍率(％) 6.00 0.03 0.10 0.00 1.7 0.2 GNT GNT含量 (mg) GNT濃度(重量 %) GNT含量 (mg) GNT濃度 (重量％) GNT回收 率(％) GNT濃縮 倍率(％) 4.60 0.02 0.21 0.01 0.01 0.4 DHG DHG含量 (mg) DHG濃度(重量 %) DHG含量 (mg) DHG濃度 (重量％) DHG回收 率(％) DHG濃縮 倍率(％) 32.7 0.11 6.96 0.21 21.3 1.5 GLY GLY含量 (mg) GLY濃度(重量 %) GLY含量 (mg) GLY濃度 (重量％) GLY回收 率(％) GLY濃縮 倍率(％) 41.80 0.21 21.97 0.99 52.6 4.7 DHG DHG含量 (mg) DHG濃度(重量 %) DHG含量 (mg) MG濃度 (重量％) DHG回收 率(％) DHG濃縮 倍率(％) 0.6 0.00 0.00 0.00 0.00 0.⑻ DHG為二氫金雀素黃酮； 質素黃醐；GNT為 GLY為黃豆素黃酮；DHG為二氫黃豆 金雀素黃酸I 素黃酮。 念異t Ϊ iHg)表示’經使气产含EQL大豆胚軸發酵物的脫脂粉末pog}或回 卒取^(2200mg)所含各異黃酮之總重量，各異黃酮濃度（重量％)表示經使用 ^含EQL大豆胚抽發酵物或回收之萃取物所含各異黃_比例。 -ΐΐί指，令含EPL之大豆胚抽發酵物所、含各成分濃度為1而算出 之固態狀卒取物中之各成分濃度的比率。 從此結果得知，即使預先將含雌馬齡之大豆胚軸發酵 物作脫脂處理，仍與前述實施例1-2相同，可以高回收率獲 得雌馬紛。The content of each xanthine (mg) represents the total weight of each isoflavone contained in the EQL soybean hypocotyl fermentate (30 g) or the recovered extract 10 (8000 mg), and the concentration of each isoflavone (weight. / .) shows the proportion of each isoflavone contained in the EQL-containing soybean hypocotyl fermentate or the recovered extract. In addition, the concentration ratio is a ratio of the concentration of each component in the solid extract obtained by making the concentration of each component contained in the soybean hypocotyls containing EQL 1. 66 200906315 Table 8 Extraction results of 90% by volume aqueous ethanol solution 5 Weight of extracts containing semi-solids containing EQL soybeans for extraction (g) 30 Dry weight (mg) 4100 EQL EQL content concentration EQL content EQL concentration EQL ® EQUIPS (mg) (% by weight) (mg) (% by weight) Rate (%) Magnification 161.40 0.54 158.13 3.89 98.0 7.2 DZN DZN content DZN concentration DZN content DZN concentration DZN recovery DZN concentration (mg) (% by weight) (mg (% by weight) Rate (%) Magnification (%) 6.00 0.02 0.00 0.00 0.0 0.0 DHD DHD content DHD concentration DHD content DHD concentration DHD recovery DHD concentration (mg) (% by weight) (mg) (% by weight) Rate (%) Magnification (%) 9.00 Ί 0.03 0.12 0.00 1.3 0.1 GNT GNT content GNT concentration GNT content GNT concentration GNT recovery GNT concentration (mg) (% by weight) (mg) (% by weight) Rate (%) Magnification (%) 6.90 0.02 0.34 0.01 4.9 0.4 DHG DHG content DHG concentration DHG content DHG concentration DHG recovery DHG concentration (mg) (% by weight) (mg) (% by weight) Rate (%) Magnification (%) 32.7 0.11 6.89 0.17 21.1 1.4 GLY GLY content GLY concentration GLY content GLY concentration GLY recovery GLY concentration (mg) (% by weight) (mg) (% by weight (%) Magnification (%) 62.70 0.21 32.64 0.80 52.1 3.8 DHG DHG content DHG concentration DHG content dug concentration DHG recovery DHG concentration (mg) (% by weight) (mg) (% by weight) Rate (%) Magnification (%) 0.9 0.00 0.00 0.00 0.00 0.00 - '-; J5, 'X-ZN gold table D back to D hydrogen 8) in each series •, two (m content rate is expected to be The yeast is taken from g) and the rDHsom axis is concentrated; the yellow 10 embryo, the Q-iq is different (4 beans are solid and the E yellow is larger than the Β5). Quality D] Fermentation contains wood·········································································· GN Huang Jing makes •, hydrogen R is the same as two g to show yellow (30 Phage HG%). Taking the extract of E t yellow from the finch net as a prime, and using it back, it is known from this result that if the equol-containing soy 10 hypocotyl fermented product is extracted with an aqueous solution of ethanol, the female can be obtained with high recovery. In addition to the phenol, yellow flavonoids can also be obtained. On the other hand, other isoflavones are extremely low in the extract. The preparation of the equol-containing extract was carried out in the same manner as in the above Reference Example 1-3 except that the powdery soybean embryos of the same batch as the above Reference Example 1-3 were produced by the first method. Soybean hypocotyl ferment. To the powdery equol-containing soybean hypocotyl fermentate 5 加入, pure 67 200906315 water was added in 7 ml, and then 250 ml of n-hexane was further added and stirred at room temperature for 7 hours. The insoluble matter was recovered by filtration, and further dried at 60 t for 7 hours to obtain 46.6 g of a defatted powder of the equol-containing soybean hypocotyl fermentate. 20 g of the aforementioned defatted powder was added to an 80% by volume aqueous solution of ethanol (ethanol was added to 100 ml of water 5) or a 90% by volume aqueous solution of ethanol (ethanol was added to 1 〇〇ml in 1 ml of water) to obtain Stir at room temperature for 14 hours. Thereafter, the residue is removed by filtration, and the filtrate and the washing liquid are recovered by washing the residue with about 100 ml of the ethanol aqueous solution having the same composition as the extraction solvent to obtain an extract. Then' at 60. (: The obtained extract was dried for 6 hours to obtain a solid extract 10. The weight of the solid extract obtained by the test and the embossing test, lignin, diterpene lignin, genius, flavonoids, The concentration of hydrogen galactoflavonoids, daidzein yellow _, and dihydrodasin flavonoids. The results are shown in Tables 9 and 10. 200906315 Table 9 Extraction results of 80% by volume aqueous ethanol solution Degreasing with EQL soybean hypocotyl fermentate Powder recovered solid extract weight for extraction fe) 20 Dry weight (mg) 4000 EQL EQL content (mg) EQL concentration (% by weight) EQL content (mg) HJL concentration (% by weight) EQL recovery (%) EQL concentration ratio 107.60 0.54 114.89 2.88 106.8 5.3 DZN DZN content (mg) DZN concentration (% by weight) DZN content (mg) DZN concentration (% by weight) DZN recovery (%) DZN concentration ratio (%) 4.00 0.02 0.00 0.00 0.0 0.0 DHD DHD content (ms) DHD concentration (% by weight) DHD content (mg) DHD concentration (% by weight) DHD recovery (%). DHD concentration ratio (%) 6.00 0.03 0.10 0.00 1.7 0.1 GNT GNT content (mg) GNT concentration (% by weight) GNT content (mg) GNT concentration (% by weight) GNT recovery rate (%) GNT concentration ratio (%) 4.60 0.02 0.28 0.01 0.01 0.4 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) DHG concentration (% by weight) DHG recovery rate (% DHG concentration ratio (%) 32.7 0.11 6.96 0.13 21.3 0.9 GLY GLY content (mg) GLY concentration (% by weight) GLY content (mg) GLY concentration (% by weight) GLY recovery rate (%) GLY concentration ratio (%) 41.80 0.21 24.03 0.60 57.5 2.9 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) DHG concentration (% by weight) DHG recovery rate (%) DHG concentration ratio (%) 0.6 0.00 0.00 0.00 0.00 0.00 In the table, EQL is Ephedrine; DZN is lignin flavone; DHD is dihydro lignin flavone; GNT is triadin flavonoid; DHG is dihydrotin flavonoid; GLY is daidzein; DHG is dihydrodain yellow. 5 The content of each isoflavone (mg) is the total weight of each isoflavone contained in the degreased powder (20 g) containing the EQL soybean hypocotyl fermentate or the recovered extract (4000 mg), and the concentration of each isoflavone (% by weight) ) indicates the ratio of the different yellow wines contained in the EQL-containing soybean hypocotyl fermentate or the recovered extract. In addition, the concentration ratio means that the concentration of each component of the soybean hypocotyl fermented product containing EQL is 1 and The ratio of the concentration of each component in the solid extract was calculated. 69 200906315 Table 10 Extraction results of 90% by volume aqueous ethanol solution Weight of solid extract recovered from degreased powder containing EQL soybean hypocotyl fermentate for extraction (g) 20 Dry weight (mg) 2200 EQL EQL content ( Mg) EQL concentration (% by weight) EQL content (mg) EQL concentration (% by weight) EQL recovery (%) EQL concentration ratio 107.60 0.54 106.88 4.81 99.3 8.9 DZN DZN content (mg) DZN concentration (% by weight) DZN content (mg DZN concentration (% by weight) DZN recovery (%) DZN concentration ratio (%) 4.00 0.02 0.00 0.00 0.0 0.0 DHD DHD content DHD concentration (% by weight) DHD content (mg) DHD concentration (% by weight) DHD recovery rate (% DHD concentration ratio (%) 6.00 0.03 0.10 0.00 1.7 0.2 GNT GNT content (mg) GNT concentration (% by weight) GNT content (mg) GNT concentration (% by weight) GNT recovery (%) GNT concentration ratio (%) 4.60 0.02 0.21 0.01 0.01 0.4 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) DHG concentration (% by weight) DHG recovery (%) DHG concentration ratio (%) 32.7 0.11 6.96 0.21 21.3 1.5 GLY GLY content (mg GLY concentration (% by weight) GLY content (mg) GLY concentration (% by weight) GLY recovery rate (%) GLY concentration ratio (%) 41.80 0.21 21.97 0.99 52.6 4.7 DHG DHG content (mg) DHG concentration (% by weight) DHG content (mg) MG concentration (% by weight) DHG recovery rate (% DHG concentration ratio (%) 0.6 0.00 0.00 0.00 0.00 0. (8) DHG is dihydromethrin flavonoids; quality jaundice; GNT is GLY for flavonoids; DHG is dihydro-glycin glucosinolate.念异t Ϊ iHg) indicates the total weight of each isoflavone contained in the defatted powder pog of the EQL soybean hypocotyl fermentate or the return stroke (2200 mg), and the concentration of each isoflavone (% by weight) is expressed. The content of each yellow-containing ratio contained in the extract containing EQL soybean embryo extract or recovered is used. - ΐΐί refers to the ratio of the concentration of each component in the solid-shaped stroke in the EPL-containing soybean embryo extract containing the concentration of each component. From this result, it was found that even if the soybean-derived hypocotyl fermented product containing the estrogen was degreased in advance, as in the above-mentioned Example 1-2, the female horse was obtained at a high recovery rate.

除了使用與前述參考例1_3不同批之粉末狀大豆胚軸 外’以與前述參考例1-3相同之條件製造粉末狀含雌馬酚之 大 — 足胚軸發酵物（雌馬酚濃度：6.2 mg/g)。將該含雌馬酚之 大 — 丑胚柏發酵物80g懸浮於水900ml，一邊授拌一邊加入乙 70 200906315 50_33g。 酸約5ml，將p_整為約4〇調整後，以室溫靜置μ小時。 之後’藉離心分離时不溶物，使其乾燥而獲得不溶物 5 於前述不溶物5G.33g中添加乙醇水溶液（乙醇浪 度75谷量％)25〇m卜以室溫授掉 汊仟Η之後，減壓過濾去除 殘造，回收濾液。接著，使 使用瘵發益使所得濾液乾涸，獲 得含雌馬酚之粉末7.53g。 ☆ 4得之含雌馬_末中含有6Q()mg/g之雌馬粉濃 又目對於起始原料之含雌馬紛大豆胚轴發酵物的M mg/g 1〇雌馬紛含量為約10倍量，雌馬紛回收率為91%。 椎馬酚之純化 馬酚 ,、使用與Θ述參考例1_3不Jgj批之粉末狀大豆胚抽以 卜二與㈣參考例1_3相同之條件，製造粉末狀含雌馬紛 15之大紅軸發酵物(雌馬紛濃度：6.2 mg/g)。將該含雌馬紛 …肢軸發酵物i〇g添加至、％容量％乙醇水溶液5〇ml， ' 振盪2小時。之後’以離心分離(2500rpm，25分鐘） 去除殘以下，記為殘以），时萃取液(以下記為萃取液 卜接著，對殘逢！添加5〇容量％乙醇水溶液5_，以室 ./盪】、時。之後，以離心分離(25〇Orpm，25分鐘)去除 爱-回收萃取液(以下記為萃取液八_2)。接著，混合萃取 液…與萃取液A-2，使用蒸發器使其乾涵，獲得殘留物(以 下記為殘留物1 )。 ;⑴述所付殘留物J中添加約50ml乙醇於室溫進行超 71 200906315 音波處理-邊進行振i。之後，以離心分離（iG術pm，ι〇 分鐘)去除殘:¾，回收萃取液(以下記為萃取液B)。使用蒸發 器使所得萃取液B乾酒，獲得殘留物（以下記為殘留物2)約 lg。 5 接著，於1g殘留物2添加己燒l〇m卜以室溫振蘆30分 鐘。之後以離心分離（1000rpm，1〇分鐘）去除萃取液，回收 不溶物0.3g。 於所彳于不/谷物〇.3g添加己燒與二乙喊之混合液（己烧： 二乙喊=2 : 3 ;容量比)2m卜以室溫振盈3()分鐘。之後， 10以離心分離（1000卬爪，w分鐘）去除殘渣，回收萃取液。將 所得萃取液於下述條件下供予二氧化矽凝膠層析。 :於内徑2cm之管柱中充填二氧化矽凝膠。 移動層:依序流動 己烷與二乙醚之混合液(己烷：二乙鍵=4〇 : 8〇 ;容量比)2〇〇mL ; 15己烧與二乙喊之混合液(己烧：二乙蜮=30 : 70 ;容量比)2〇〇mL ; 己烷與二乙醚之混合液(己烷：二乙醚=25 : 75 ;容量比)2〇〇mL ; 己院與二乙醚之混合液(己烷：二乙醚=2〇 : 8〇 ;容量比)2〇〇mL ; 及 己院200mL。 每50mL地回收管柱溶出之溶液。己烷與二乙醚之混合 液（己烧.一乙30 : 70 ;容量比）及己烧與二乙醚之混合 液（己燒.一乙驗=25 : 75 ;容量比）所溶出之分液合計含有 約40mg之雌馬盼（回收率go%)，其乾燥固態部份之平均雌馬 酚純度為99.9%。此外，將TLC分析本二氧化矽凝膠層析所 72 200906315 溶出分液之結果示於第8圖。如第8圖所示，確認己烷與二 乙醚之混合液（己烷：二乙醚=30 : 70 ;容量比）及己烷與二 乙醚0混合液（己烷：二乙醚二25 : 75 ;容量比）所溶出之分 液含有雌馬酚，其純化度亦甚高。此外，供予第8圖各巷之 5 試料係如表11所示。 表11 第8圖中之巷 試料内容 巷1 雌馬盼標準品 巷2 0~50mL間所溶出之分液，其成為己烷舆二乙醚之混合液（己 烷：二乙醚= 40: 80;容量比）的溶出量 巷3 50〜100mL間溶出之分液，其成為己烷與二乙醚之混合液（己 烧：二乙醚= 40: 80;容量比）的溶出量 巷4 50~100mL間溶出之分液，其成為己烷與二乙醚之混合液（己 烷：二乙醚= 30: 70;容量比）的溶出量 巷5 0~50mL間溶出之分液，其成為己烷與二乙醚之混合液（己 烷：二乙醚= 30: 70;容量比）的溶出量 巷6 0〜50mL間溶出之分液，其成為己烷與二乙醚之混合液（己 烧：二乙醚=25 : 75 ;容量比）的溶出量 巷7 150〜200mL間溶出之分液，其成為己烷與二乙醚之混合液（己 烷：二乙醚=25 : 75 ;容量比）的溶出量 巷8 0〜50mL間之溶出之分液，其成為己烷舆二乙醚之混合液（己 烷：二乙醚=20 : 80 ;容量比）的溶出量 實施例2-2 : 以第2方法純化雌馬酚 除使用與前述參考例1-3不同批之粉末狀大豆胚軸以 外，以與前述參考例1-3相同之條件製造粉末狀含雌馬酚之 10 大豆胚軸發酵物（雌馬紛濃度：6.2 mg/g)。將該含雌馬盼之 大豆胚軸發酵物100g添加於95容量％乙酸乙酯水溶液 500ml，以室溫攪拌4小時。之後，濾別不溶物，更以80ml 乙酸乙酯洗淨該不溶物。將所得洗淨液與濾液一起減壓濃 縮，回收12g油狀物。使所得油狀物12g於下述條件下供予 15 二氧化矽凝膠層析。 管柱：使用己烷於内徑2cm之管柱填充二氧化矽凝膠 70g。 73 200906315 移動層：流通己烷500ml後，流通乙酸乙酯與己烷之混 合液（乙酸乙酯：己烷=1 : 5(容量比））。 從如此回收之含雌馬紛溶出液顧除溶劑，而獲得黃色 結晶狀之固態物630mg。 將所得黃色結晶狀固態物添加於乙酸乙酯2ml並加熱 至約80 C使其溶解，更添加己烧8mh見合後’冰冷下授掉30 分鐘，使雌馬酚結晶析出。過濾回收析出之雌馬酚結晶， 將其以己烷洗淨後，乾燥而獲得含雌馬酚之固態部分 530mg(淡黃色結晶）。 再將該淡黃結晶530mg加入4ml乙醇水溶液（乙醇濃度 62.5容量％)，於約8〇。〇力σ熱溶解，再以1〇。〇靜置12小時， 以進行雌馬酚之再結晶化，進行過濾及乾燥，獲得白色針 狀雌馬酚結晶390mg。 15 所得雌馬紛結晶之乙醇25t的旋光度[α]〇25為_21.6。， 融點為购赃，而確認已純化出將近魏純度之雕馬 氣〜，…一 -☆-喟哪巧m 含有其之舍' 20 製造含有含雌馬紛 塊之食品素材 之大豆駐軸發酵物與可可 3(重量比）之比率混 齡大豆胚軸發酵物 粉之大豆胚軸發酵 於使可可塊加熱熔融之狀態，以1: 合以參考例1-3相同條件製造之含雌馬 以及可可塊後，使其冷卻而製得含雖馬 物分散於可可塊中之食品素材。 74 200906315 以π)位官能檢查員就如此製造之食品素材的味覺呈現 力口以評估’所有的官能檢查㈣評估為：源自含雌馬酴之 大丑胚軸發酵物的苦味已受到抑制而風味良好。 纟有含雌馬狀大豆胚軸發酵物 食品素材 於使可可塊加熱炫融之狀態下’以i: 24(重量比)之比 率混合實施例μ巾制75Et所獲得之萃取液2]乾涵而成 的^態狀萃取物（以下記為75Et萃取物)與可可塊後，冷卻而 獲得75Et萃取物分散於可可塊中之食品素材。 1〇 ⑽位官能檢查員就如此製造之食品素材的味覺呈現 加以評估，所有的官能檢查員均評估為：源自勘萃取物 的苦味已受到抑制而風味良好。 复座例3-3 烘烤點心 將表12所示原料，以成型機使所得昆合原料成形為長 15*^m.5Cmxl.5Cmx10cm)，m8(rc_n ^ 菓子。 以H)位官能檢查員就所得烘烤點心的味覺呈現加以評 估，所有的官能檢查員均評估為：源自含雌 立胚 轴發酵物的苦味受到抑制，可感受到良好之巧克力風味， 20並可感到烘烤點心原本之良好味覺呈現。 75 200906315 表12 配合量(重量％) 片狀之實施例3-1所得食品素材 (每片平均重量約〇.lg) 5 大豆粉 25 葡萄乾 適量 奶油 適量 砂糖 適量 蛋 適量 柑橘皮 適量 鳳梨 適量 難消化性糊精 適量 可可塊 適量 杏仁碎粒 適量 可可粉 適量 食鹽 適量 膨脹劑 適量 香料 適量 全量 100 比較例3-1 烘烤點心 除了添加巧克力片5g及與實施例1-3相同條件製造之 含雌馬酚大豆胚軸發酵物lg以取代片狀之實施例3-1所得 5 食品素材以外，以與前述實施例3-3相同之條件製造烘烤點 心〇 以10位官能檢查員就點心之味覺呈現加以評估，所有 的官能檢查員均評估為：味覺呈現受損，源自含雌馬酚之 大豆胚軸發酵物的苦味受到擴散到整體中。 10 實施例3-4 烘烤點心 除了等量片狀之實施例3-2所得食品素材以取代片狀 之實施例3-1所得食品素材以外，以與前述實施例3-3相同之 條件製造烘烤點心。 以10位官能檢查員就所得烘烤點心的味覺呈現加以評 15 估，所有的官能檢查員均評估為：源自75Et萃取物之苦味 76 200906315 受到抑制’可感受到良好之巧克力風味 心原本之良好味覺呈現。 並可感到烘烤點 細4加含雌 種含品 ~~~~:物的各· 5 實施例4-A 飲料 列舉具體例。 以下，就本發明飲料之製造例 實施例4-A-1 氣泡水 調製下述組成之薄荷風味糖_ 液與碳酸水以3 : 1之容量比混合，择專何風味糖聚 10薄荷風味糖栽液之組成 氣&水。A powdery equol-containing foot-fertilis fermented product (equol concentration: 6.2) was produced except that powdery soybean hypocotyls different from the above-mentioned Reference Example 1-3 were used. Mg/g). 80 g of the equol-containing large ugly cypress fermented product was suspended in 900 ml of water, and B 70 200906315 50-33 g was added while mixing. The acid was about 5 ml, and the p_ was adjusted to about 4 Torr, and then allowed to stand at room temperature for μ hours. Then, 'insoluble matter by centrifugation, and dried to obtain insoluble matter 5. Adding an aqueous solution of ethanol (ethanol wave 75%) to the above insoluble matter 5G.33g 25 〇m b after the 授 is given at room temperature The residue was removed by filtration under reduced pressure, and the filtrate was recovered. Next, the obtained filtrate was dried using a hydrazine to obtain 7.53 g of a equol-containing powder. ☆ 4 containing the female horse _ the end contains 6Q () mg / g of the female horse powder thick and the eye of the starting material containing the female horse soybean hypocotyl fermented product M mg / g 1 〇 female horse content is About 10 times the amount of female horses recovered 91%. Purification of p-paraffin, and use of the same conditions as described in Reference Example 1_3, not powdered soybean embryos of Jgj batch, and (4) reference example 1_3, to produce a powdery red shaft ferment containing a female horse 15 (Equine concentration: 6.2 mg/g). The equine-containing limb shaft fermented product i〇g was added to a 5 % by volume aqueous solution of % capacity %, and was shaken for 2 hours. After that, the residue was removed by centrifugation (2,500 rpm, 25 minutes), and the extract was counted as the extract (hereinafter referred to as the extract). Next, add 5 〇% by volume of the aqueous ethanol solution to the chamber. Afterwards, the mixture was centrifuged (25 〇 O rpm, 25 minutes) to remove the love-recovery extract (hereinafter referred to as extract _2). Then, the extract was mixed with extract A-2, using evaporation. The apparatus was allowed to dry and culvert to obtain a residue (hereinafter referred to as residue 1). (1) Adding about 50 ml of ethanol to the residue J described above was carried out at room temperature for super 71 200906315. The sonication was carried out while the vibration was performed. Separation (iG pm, ι min) removes the residue: 3⁄4, and recovers the extract (hereinafter referred to as extract B). The obtained extract B is dried using an evaporator to obtain a residue (hereinafter referred to as residue 2). Lg. 5 Next, 1 g of the residue 2 was added to the mixture, and the mixture was shaken at room temperature for 30 minutes. Thereafter, the extract was removed by centrifugation (1000 rpm, 1 minute) to recover 0.3 g of insoluble matter. No / cereal 〇. 3g added a mixture of burned and simmered (already burned: two shouted = 2: 3; capacity 2 m was shaken at room temperature for 3 () minutes. Thereafter, the residue was removed by centrifugation (1000 jaws, w minutes), and the extract was recovered. The obtained extract was supplied to cerium oxide gel chromatography under the following conditions. : Filling a column with an inner diameter of 2 cm with a cerium oxide gel. Moving layer: a mixture of hexane and diethyl ether (hexane: two-bond = 4 〇: 8 〇; capacity ratio) 2 依〇mL ; 15 mixture of hexane and 2 喊 己 (hex: 二 蜮 = 30: 70; volume ratio) 2 〇〇 mL; a mixture of hexane and diethyl ether (hexane: diethyl ether = 25: 75; capacity ratio) 2 〇〇 mL; a mixture of hexaxy and diethyl ether (hexane: diethyl ether = 2 〇: 8 〇; volume ratio) 2 〇〇 mL; and 200 mL of the hospital. Recycling column every 50 mL Solution solution: a mixture of hexane and diethyl ether (hexane, a 30:70; volume ratio) and a mixture of hexane and diethyl ether (hexane, one test = 25: 75; capacity ratio) The total fraction of the dissolved fractions contained about 40 mg of equuma (recovery rate%), and the average equol purity of the dried solid portion was 99.9%. In addition, TLC analysis of the cerium oxide gel chromatography system 72 200906315 Dissolve The results of the liquid separation are shown in Fig. 8. As shown in Fig. 8, a mixed liquid of hexane and diethyl ether (hexane: diethyl ether = 30:70; volume ratio) and a mixture of hexane and diethyl ether ( Hexane: diethyl ether 2:25:75; volume ratio) The fractions dissolved contained equol, and the degree of purification was also very high. Further, the samples of the 5 samples supplied to the lanes of Fig. 8 are shown in Table 11. 11 Figure 8 Lane sample content Lane 1 Female horses looking for the separation of the standard product lane between 0 0 ~ 50mL, which becomes a mixture of hexane 舆 diethyl ether (hexane: diethyl ether = 40: 80; capacity The ratio of the dissolution amount of the lane 3 to 50~100mL, which is dissolved in a mixture of hexane and diethyl ether (hexane: diethyl ether = 40: 80; capacity ratio) of the dissolution amount of 4 50 ~ 100mL dissolution The liquid separation is carried out as a mixture of hexane and diethyl ether (hexane: diethyl ether = 30:70; capacity ratio), and the solvent is eluted between 50 and 50 mL, which becomes hexane and diethyl ether. A solution of the mixed solution (hexane: diethyl ether = 30:70; capacity ratio) is dissolved in a mixture of 60 to 50 mL, which is a mixture of hexane and diethyl ether (alloyed: The amount of elution in the range of 7 150 to 200 mL of the elution amount of diethyl ether = 25:75; the ratio of dissolution to the mixture of hexane and diethyl ether (hexane: diethyl ether = 25:75; capacity ratio) Dissolution of the mixture between 80 and 50 mL of the lane, which is a mixture of hexane oxime diethyl ether (hexane: diethyl ether = 20:80; capacity ratio). Example 2-2: Purification by the second method Eryl phenol was prepared as a powdery equol-containing 10 soybean hypocotyl fermentate (equal horse) except that the powdery soybean hypocotyls different from the above-mentioned Reference Example 1-3 were used in the same manner as in the above Reference Example 1-3. Concentration: 6.2 mg/g). 100 g of the soybean hypocotyl fermented product containing equol was added to 500 ml of a 95% by volume aqueous ethyl acetate solution, and the mixture was stirred at room temperature for 4 hours. Thereafter, the insoluble matter was filtered, and the insoluble material was washed with 80 ml of ethyl acetate. The obtained washing liquid was concentrated under reduced pressure with a filtrate, and 12 g of oil was collected. 12 g of the obtained oil was subjected to 15 cerium oxide gel chromatography under the following conditions. Column: 70 g of cerium oxide gel was filled with hexane on a column of 2 cm inner diameter. 73 200906315 Moving layer: After flowing 500 ml of hexane, a mixed liquid of ethyl acetate and hexane (ethyl acetate:hexane = 1:5 (capacity ratio)) was passed. From the thus-recovered soy-containing eluate, the solvent was removed to obtain 630 mg of a solid yellow crystal. The obtained yellow crystalline solid was added to 2 ml of ethyl acetate and heated to about 80 C to dissolve it. After adding 8 hours of hexane, it was allowed to stand for 30 minutes under ice cooling to precipitate equol. The precipitated equol crystals were collected by filtration, washed with hexane, and dried to obtain 530 mg (yellow crystal) of a solid portion containing equol. Further, 530 mg of the pale yellow crystals were added to 4 ml of an aqueous ethanol solution (ethanol concentration: 62.5 vol%) at about 8 Torr. 〇 σ 热 heat dissolved, and then 1 〇. The mixture was allowed to stand for 12 hours to carry out recrystallization of equol, followed by filtration and drying to obtain 390 mg of white needle-like equol crystals. 15 The optical rotation of the obtained female equine crystallization of 25t [α] 〇 25 is _21.6. The melting point is purchased, and it is confirmed that the purity of the near-purity of the horse has been purified~,... one-☆-喟 巧 巧 含有 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 The ratio of the fermented product to the cocoa 3 (weight ratio) is mixed with the soybean hypocotyl fermented powder of the soy hypocotyl fermented powder in a state in which the cocoa mass is heated and melted, and the female horse is produced in the same condition as in Reference Example 1-3. After the cocoa mass is cooled, the food material containing the horse material dispersed in the cocoa mass is prepared. 74 200906315 The π) positional inspector showed the taste of the food material so produced to evaluate 'all functional tests (4) as: the bitterness derived from the fermented material of the ugly hypocotyls containing the female horse has been suppressed. Good flavor.纟 雌 含 雌 胚 胚 胚 胚 胚 胚 胚 胚 胚 胚 胚 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌 雌The obtained state-like extract (hereinafter referred to as 75Et extract) and the cocoa mass were cooled to obtain a food material in which 75Et of the extract was dispersed in the cocoa mass. 1〇 (10) The functional inspector evaluated the taste profile of the food material thus produced, and all the functional inspectors evaluated that the bitterness derived from the extract was suppressed and the flavor was good. Compound 3-3 Baking snacks The raw materials shown in Table 12 were formed into a length of 15*^m.5Cmxl.5Cmx10cm), m8(rc_n^ fruit. H) functional inspector by a molding machine. The taste perception of the obtained baked snacks was evaluated, and all the functional inspectors evaluated that the bitterness derived from the fermented product containing the female hypocotyls was suppressed, and a good chocolate flavor was felt, 20 and the original baked snack was felt. A good taste is presented. 75 200906315 Table 12 Compounding amount (% by weight) The food material obtained in Example 3-1 (the average weight of each tablet is about 〇.lg) 5 Soy flour 25 raisin amount of cream, amount of granulated sugar, moderate amount of citrus peel, appropriate amount of pineapple, indigestible Amount of dextrin, a proper amount of cocoa, an appropriate amount of almonds, an appropriate amount of cocoa powder, an appropriate amount of salt, an amount of swelling agent, an appropriate amount of the flavor, and a full amount of 100. Comparative Example 3-1 Baked snacks, except for the addition of chocolate chips 5g and the same conditions as those of Examples 1-3. The phenolic soybean hypocotyl fermented product lg was prepared in the same manner as in the above Example 3-3 except that the food material obtained in Example 3-1 was replaced with the sheet material, and the taste of the snack was presented by the 10-member inspector. As assessed, all of the panelists evaluated that the taste was impaired and that the bitter taste derived from the equol-containing soybean hypocotyl ferment was diffused into the whole. [Example 3-4] The baked foodstuff was produced in the same manner as in the above Example 3-3 except that the food material obtained in Example 3-2 was replaced with the sheet material in the same manner as in the above Example 3-3. Bake a snack. Ten taste inspectors evaluated the taste of the baked snacks, and all the functional inspectors evaluated: bitterness from the 75Et extract 76 200906315 was inhibited 'feelable good chocolate flavor original Good taste is presented. You can feel the baking point. 4 Add the female-containing product. ~~~~: Each of the objects. 5 Example 4-A Beverage Specific examples are given. Hereinafter, in the production example 4-A-1 of the beverage of the present invention, the following composition of the mint flavored sugar liquid and the carbonated water are mixed in a capacity ratio of 3:1, and the flavored sugar is gathered into 10 mint flavored sugar. The composition of the planting liquid & water.

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含雌馬紛之大豆胚軸發酵物(參考例 砂糖 j 〇.27(Kg) 檸檬酸(結晶） 0.80 食鹽 0.01 氯化鉀 0.012 乳酸鈣 0.005 歐薄荷萃取物 0.008 歐薄荷香料 0.03 1 __ 0.20 殘餘部分 氣泡水 100.00L λ犯1夕丨j卜1中使用75 後的固態狀萃取物〇.〇34 :传之卒取液Μ乾酒 酵物以調㈣荷風味糖漿㈣外敎大豆胚軸發 Γ以與刖述實施例4-A-1相 77 200906315 同之條件製造氣泡水。 實施例4-A-3 無果汁柑橘碳酸飲料 調製下述組成之柑橘風味糖漿液，將該柑橘風味糖漿 液與碳酸水以9 : 11容量比混合，獲得無果汁柑橘碳酸飲料。 5 柑橘風味糖漿液之組成 含雌馬酌·之大豆胚轴發酵物（參考例1-3) 0.20(Kg) 砂糖 10.00 檸檬酸(結晶） 0.03 DL-蘋果酸 0.07 10 著色劑 0.05 香料 0.10 ^_殘餘部分Soybean hypocotyl fermented product containing female horses (Reference Example Sugar j 〇.27 (Kg) Citric acid (crystal) 0.80 Salt 0.01 Potassium chloride 0.012 Calcium lactate 0.005 Peppermint extract 0.008 Spearmint flavor 0.03 1 __ 0.20 Residue Bubble water 100.00L λ 1 1 丨 丨 j 卜 1 After using 75 solid extract 〇. 〇 34: passed the stroke liquid Μ dry wine yeast to adjust (four) scented syrup The bubble water was produced under the same conditions as in the description of Example 4-A-1 phase 77 200906315. Example 4-A-3 Juice-free citrus carbonated beverage The citrus-flavored syrup of the following composition was prepared, and the citrus-flavored syrup and carbonated The water is mixed in a ratio of 9:11 to obtain a juice-free citrus carbonated beverage. 5 The composition of the citrus-flavored syrup contains the soybean hypocotyl fermented product of the female horse (Reference Example 1-3) 0.20 (Kg) Sugar 10.00 Citric acid ( Crystallization) 0.03 DL-malic acid 0.07 10 coloring agent 0.05 fragrance 0.10 ^_ residual

合計 45.00L 實施例4-A-4 無果汁柑橘碳酸飲料 15 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇25Kg來取代含雌馬酚之大豆胚轴發 酵物以調製柑橘風味糖漿液以外，以與前述實施例4-A-3相 同之條件無果汁柑橘碳酸飲料。 實施例4-A-5 低卡可樂 20 調製下述組成之可樂糖漿液，將該可樂糖漿液與碳酸 水以8 : 12容量比混合，獲得無糖可樂。 可樂糖漿液之組成 含雌馬酌·之大豆胚軸發酵物（參考例1-2) 0.20(Kg) 蔗糖素 0.02 78 200906315 磷酸 0.05 檸檬酸(結晶） 0.07 咖σ非因 0.10 焦糖色素 0.20 香料(可樂香料） 0.10 ^_殘餘部分Total 45.00 L Example 4-A-4 Juice-free citrus carbonated beverage 15 In addition to the solid extract of 萃取.〇25Kg, which was used in the extraction of 1-2 5% of the extract 7.5 obtained in Example 1-1, the equol was replaced by equol. The soybean hypocotyl fermentate was prepared in the same manner as in the above Example 4-A-3 except that the citrus flavor syrup was prepared. Example 4-A-5 Low Coke 20 A cola syrup of the following composition was prepared, and the cola syrup was mixed with carbonated water at a ratio of 8:12 to obtain a sugar-free cola. The composition of the cola syrup contains the fermented soybean hypocotyl fermented product (Reference Example 1-2) 0.20 (Kg) Sucralose 0.02 78 200906315 Phosphoric acid 0.05 Citric acid (crystal) 0.07 Coffee σ non-0.10 Caramel color 0.20 Spice (Coke Spice) 0.10 ^_Residuals

合計 40.00L 實施例4-A-6 低卡可樂 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.03Kg來取代含雌馬酚之大豆胚軸發酵 物以調製可樂糖漿液以外，以與前述實施例4-A-5相同之條 件製造低卡可樂氣泡水。 實施例4-A-7 蘋果碳酸飲料 15 調製下述組成之蘋果風味糖漿液，以13 : 7容量比混合 該蘋果風味糖漿液與碳酸水，獲得蘋果碳酸飲料。 蘋果風味糖漿液之組成 20Total 40.00 L Example 4-A-6 Low Coke 10 In place of the equol-containing soybean hypocotyls in addition to the solid extract of 0.03 Kg after the dry extraction of 1-2 extracts obtained in Example 1-1 using 75Et The fermented material was used to prepare low-carbon cola bubble water in the same conditions as in the above-mentioned Example 4-A-5 except that the cola syrup was prepared. Example 4-A-7 Apple carbonated beverage 15 An apple flavored syrup having the following composition was prepared, and the apple flavored syrup and carbonated water were mixed at a capacity ratio of 13:7 to obtain a apple carbonated beverage. Composition of apple flavored syrup 20

含雌馬酚之大豆胚軸發酵物（參考例1-2) 〇.2〇(Kg) 5倍濃縮蘋果果汁 11.00 果糖葡萄糖液 5.00 挣橡酸(結晶） 0.10 著色劑 0.05 香料 0.10 水 殘餘部分 合計 85.00L 79 200906315 實施例4-A-8 頻果礙酸飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇3Kg來取代含雌馬粉之大豆胚軸發酵 物以調製蘋果風味糖漿液以外，以與前述實施例4-A-7相同 5 之條件製造蘋果碳酸飲料。 實施例4-A-9 匍萄碳_酸飲料 調製下述組成之葡萄風味糖漿液，並以9 : 11容量比混 合該葡萄風味糖漿液與碳酸水，獲得葡萄碳酸飲料。 葡萄風味糖漿液之組成 10 含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.20(Kg) 5倍濃縮葡萄透明果汁 4.40 檸檬酸(結晶） 0.25 著色劑 0.05 香料 0.20 15 ^_殘餘部分Erythromycin-containing soybean hypocotyl ferment (Ref. 1-2) 〇.2〇(Kg) 5 times concentrated apple juice 11.00 fructose glucose solution 5.00 earned rubber (crystal) 0.10 colorant 0.05 fragrance 0.10 water residue total 85.00L 79 200906315 Example 4-A-8 The acid-salted beverage is replaced with the solid extract of 萃取.〇3Kg in the extract 1-2 obtained in Example 1-1 using 75Et. The soybean hypocotyl fermentate was prepared in the same manner as in the above Example 4-A-7 except that the apple flavor syrup was prepared. Example 4-A-9 Carbon-acid beverage A grape-flavored syrup having the following composition was prepared, and the grape-flavored syrup and carbonated water were mixed at a ratio of 9:11 to obtain a grape-carbonated beverage. Composition of grape-flavored syrup 10 Estradiol-containing soybean hypocotyl ferment (Reference 1-1) 0.20 (Kg) 5 times concentrated grape transparent juice 4.40 Citric acid (crystal) 0.25 Colorant 0.05 Spice 0.20 15 ^_ Residue section

合計 45.00L 實施例4-A-10 葡萄碳酸飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇3Kg來取代含雌馬酚之大豆胚轴發酵 20 物以調製葡萄風味糖漿液以外，以與前述實施例4-A-1相同 之條件製造葡萄碳酸飲料。 實施例4-A-11 蘋果汁飲料 製造下述組成之蘋果汁飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 80 200906315 5倍濃縮蘋果果汁 22.00 香料 0.05 ^_殘餘部分A total of 45.00 L Example 4-A-10 Grape carbonated beverage In addition to the solid extract of 萃取.〇3Kg, which was used in the 1-1 of the extract 1-2 obtained in Example 1-1, was used to replace the equol-containing soybean embryo. A grape-carbonated beverage was produced under the same conditions as in the above Example 4-A-1 except that the shaft was fermented to prepare a grape-flavored syrup. Example 4-A-11 Apple Juice Beverage An apple juice beverage of the following composition was produced. Soybean hypocotyl ferment containing equol (Reference Example 1-3) 0.40 (Kg) 80 200906315 5 times concentrated apple juice 22.00 Spice 0.05 ^_ residual

合計 100.00L 5 實施例4-A-12 蘋果汁飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05Kg來取代含雌馬盼之大豆胚軸發酵 物以外，以與前述實施例4-A-11相同之條件製造蘋果汁飲 料。 10 實施例4-A-13 柑橘果汁飲料 製造下述組成之柑橘果汁飲料。 含雌馬酚之大豆胚轴發酵物（參考例1-3) 0.40(Kg) 濃縮瓦雷西亞柑橘果汁（布里克斯55°) 4.40 砂糖 5.00 15 檸檬酸(結晶） 0.18 負型結冷膠 0.025 維生素C 0.03 果膠 0.0025 柑橘香料 0.25 20 丞_殘餘部分A total of 100.00 L 5 Example 4-A-12 Apple juice beverage In addition to the solid extract of 0.05× of the extract 1-2 obtained in Example 1-1, the solid extract of 0.05 gram was used to replace the soybean hypocotyl containing the female horse. An apple juice beverage was produced under the same conditions as in the above Example 4-A-11 except for the ferment. 10 Example 4-A-13 Citrus Juice Beverage A citrus juice drink of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Concentrated Varesian Citrus Juice (Brix 55°) 4.40 Sugar 5.00 15 Citric Acid (Crystalline) 0.18 Negative Junction Gel 0.025 Vitamin C 0.03 Pectin 0.0025 Citrus Spice 0.25 20 丞_Residuals

合計 100.00L 實施例4-A-14 柑橘果汁飲料 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05Kg來取代含雌馬盼之大豆胚轴發酵 81 200906315 物以外，以與前述實施例4-A-13相同之條件製造柑橘果汁 飲料。 實施例4-A-15 低卡果汁飲料 製造下述組成之低卡果汁飲料。 5 含雌馬酚之大豆胚轴發酵物（參考例1-1) 0.40(Kg) 5倍濃縮柑橘果汁 4.40 蔗糖素 0.009 檸檬酸(結晶） 0.18 L-抗壞血酸 0.03 10 結冷膠 0.024 香料 0.10 ^_殘餘部分Total 100.00L Example 4-A-14 Citrus Fruit Juice Beverage In addition to the use of 75Et of the extract 1-2 obtained in 75 °, the solid extract of the dried extract is used to replace the soybean hypocotyl fermentation with the female horse. 81 200906315 A citrus juice beverage was produced under the same conditions as in the above Example 4-A-13 except for the above. Example 4-A-15 Low-calorie juice beverage A low-calorie juice beverage of the following composition was produced. 5 Erythromycin-containing soybean hypocotyl ferment (Reference 1-1) 0.40 (Kg) 5 times concentrated citrus juice 4.40 Sucralose 0.009 Citric acid (crystalline) 0.18 L-ascorbic acid 0.03 10 Gellan gum 0.024 Perfume 0.10 ^_ Residue

合計 100.00L 實施例4-A-16 低卡果汁飲料 15 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-15相同之條件製造低卡果汁 飲料。 實施例4-A-17 無糖加味水(near-water) 20 製造下述組成之無糖加味水。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 蔗糖素 0.008 聚糊精 2.20 5倍濃縮葡萄柚濃縮果汁 0.44 82 200906315Total 100.00 L Example 4-A-16 Low-calorie juice beverage 15 In place of the equol-containing solid extract 〇.5Kg in combination with the extract 1-2 obtained in Example 1-1 using 75Et of the extract 1-2. A low-calorie fruit juice beverage was produced under the same conditions as in the above Example 4-A-15 except for the soybean hypocotyl fermentate. Example 4-A-17 Sugar-free flavored water (near-water) 20 A sugar-free flavoring water of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Sucralose 0.008 Polydextrin 2.20 5 times concentrated grapefruit concentrated juice 0.44 82 200906315

檸檬酸(結晶） 0.53 乳酸鈣 0.08 氯化鉀 0.01 L-抗壞血酸 0.03 5 苯曱醯硫胺明鹽酸鹽 0.0002 0比哆醇鹽酸鹽 0.00015 菸酸醯胺 0.0015 香料 0.10 水 殘餘部分 10 合計 實施例4-A-18 無糖加味水 100.00L 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-17相同之條件製造無糖加味 15 水。 實施例4-A-19 運動飲料 製造下述組成之運動飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 〇.40(Kg) 檸檬透明果汁 0.50 20 蔗糖素 0.02 乳性礦物質 1.00 綜合維生素 0.25 L-抗壞血酸鈉 0.05 檸檬酸 0.125 83 200906315 ^_殘餘部分Citric acid (crystal) 0.53 Calcium lactate 0.08 Potassium chloride 0.01 L-ascorbic acid 0.03 5 Benzoyl thiamine hydrochloride hydrochloride 0.0002 0 sterol hydrochloride 6.0015 Nicotinic acid decylamine 0.0015 Perfume 0.10 Water residue 10 Total examples 4-A-18 Sugar-free flavored water 100.00L In addition to the solid extract of 萃取.〇5Kg, which is used in the extraction of 1-2 of the extract 1-2 obtained in Example 1-1, the equol-containing soybean hypocotyl fermentation was replaced. Sugar-free flavored 15 water was produced in the same manner as in the above Example 4-A-17 except for the above. Example 4-A Sports drink A sports drink of the following composition was produced. Soybean hypocotyl ferment containing equol (Reference 1-2) 〇.40(Kg) Lemon transparent juice 0.50 20 Sucralose 0.02 Milk mineral 1.00 Comprehensive vitamin 0.25 L-sodium ascorbate 0.05 Citric acid 0.125 83 200906315 ^ _Residual part

合計 100.00L 實施例4-A-20 運動飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 5 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-19相同之條件製造運動飲料。 實施例4-A-21 抹茶牛奶飲料 製造下述組成之抹茶牛奶。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 0.40(Kg) 10 牛奶 10.00 脫脂奶粉 3.50 抹茶((株)伊藤園製RS-20) 0.50 砂糖 7.00 乳化劑 0.70 15 著色劑 0.03 香料 0.20 碳酸氫鈉 調整至pH8.8 ^_殘餘部分Total 100.00 L Example 4-A-20 Sports Beverage In addition to the solid extract of 0.05K after extracting 1-2 dry extract 5 obtained using 75Et in Example 1-1, the equol-containing soybean hypocotyl fermentation was replaced. A sports drink was produced under the same conditions as in the above Example 4-A-19 except for the above. Example 4-A-21 Matcha Milk Beverage Matcha milk of the following composition was produced. Soybean hypocotyl fermented product containing equol (Reference Example 1-2) 0.40 (Kg) 10 Milk 10.00 Skim milk powder 3.50 Matcha (RS-20 manufactured by Ito Garden Co., Ltd.) 0.50 Sugar 7.00 Emulsifier 0.70 15 Colorant 0.03 Perfume 0.20 Sodium bicarbonate was adjusted to pH 8.8 ^_ residual

合計 100.00L 20 實施例4-A-22 抹茶牛奶飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-21相同之條件製造抹茶牛奶 飲料。 84 200906315 ^^^Ji^A-23無糖優酪乳 製造下述組成之無糖優路乳。 〇.40(Kg) 40.00 0.20 0.20 0.20 調整至pH4.2 殘餘邬公 含雌馬酚之大豆胚軸發酵物（參考例 發酵乳(SNF20%) 水溶性大豆多糖類 果膠 香料 乳酸 10 20 合計Total 100.00 L 20 Example 4-A-22 Matcha Milk Beverage In addition to the solid extract of 萃取.〇5Kg in the use of the extract obtained by using 75Et in Example 1-1, the equol-containing soybean was replaced. In addition to the hypocotyl fermentate, a matcha milk beverage was produced under the same conditions as in the above Example 4-A-21. 84 200906315 ^^^Ji^A-23 Sugar-free yogurt The following ingredients are made of sugar-free yogurt. 〇.40(Kg) 40.00 0.20 0.20 0.20 Adjusted to pH 4.2 Residual 大豆 大豆 Ephedrine Soybean Hypocotyl Fermentation (Reference Example Fermented Milk (SNF20%) Water Soluble Soy Polysaccharide Pectin Perfume Lactic Acid 10 20 Total

100.00L 實施例4-A-24 無糖優赂乳 除了配合實施例1_1中使用75Et獲得之萃取液乾酒 後的固態狀萃取物G.G5Kg來取代含雌馬紛之大豆胚轴發酵 物以外，以與前述實施例4-Α_23相同之條件製造無糖優赂 15 乳 f施例4-Α-25 滅菌乳酸菌飲料 °-4〇(Kg) 15.00 0.40 8.00 0.10 調整至pH3.8 製造下述組成之滅菌乳酸菌飲料。 含雌馬盼之大豆胚軸發酵物（參考例丨_3) 發酵乳(SNF20%) 水溶性大豆多糖類 砂糖 香料 乳酸 ^_ 85 200906315100.00L Example 4-A-24 Sugar-free yoghurt In addition to the solid extract G.G5Kg after the dry extract of the extract obtained by using 75Et in Example 1_1, the soybean-containing hypocotyl fermented product was replaced. Manufactured in the same conditions as in the above Example 4-Α_23, the sugar-free glutinous rice 15 milk f Example 4-Α-25 sterilized lactic acid bacteria beverage °-4 〇 (Kg) 15.00 0.40 8.00 0.10 Adjusted to pH 3.8 The following composition was produced. Sterilized lactic acid bacteria beverage. Soybean hypocotyl fermented product containing female horses (Reference Example 33) Fermented milk (SNF20%) Water-soluble soybean polysaccharides Sugar Spices Lactic acid ^_ 85 200906315

合計 100.00L 實施例4-A-26 滅菌乳酸菌飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 5 物以外，以與前述實施例4-A-25相同之條件製造滅菌乳酸Total 100.00 L Example 4-A-26 Sterilized Lactic Acid Bacteria Drink In addition to the solid extract of 萃取.〇5Kg, which was used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean embryo was replaced. The sterilized lactic acid was produced under the same conditions as in the above Example 4-A-25 except for the shaft fermentation.

菌飲料。 實施例4-A-27 低卡酸轧飲料 製造下述組成之低卡酸乳飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 10 脫脂奶粉 3.50 水溶性大豆多糖類 0.40 砂糖 5.00 蔗糖素 0.0008 果糖葡萄糖液(75%) 5.00 15 檸檬酸(結晶） 0.45 香料 0.10 乳酸 調整至pH3.8 水 殘餘部分 合計 100.00L 20 實施例4-A-28 低卡酸乳飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-27相同之條件製造低卡酸乳 飲料。 86 200906315 實施例4-A-29 草莓牛奶飲料 製造下述組成之草莓牛奶飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 脫脂奶粉 2.80 5 全脂奶粉 3.00 砂糖 12.00 乳化劑 0.08 著色劑 0.05 香料(草莓香料） 0.10 1〇 丞_殘餘部分Bacterial drink. Example 4-A-27 Low-calorie rolled beverage A low-calorie yogurt beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) 10 Skim milk powder 3.50 Water-soluble soybean polysaccharide 0.40 Sugar 5.00 Sucralose 0.0008 Fructose glucose solution (75%) 5.00 15 Citric acid (crystallization) 0.45 Fragrance 0.10 Lactic acid adjusted to pH 3.8 Water residue total 100.00 L 20 Example 4-A-28 Low caloric acid beverage In addition to the solid solution after the use of 75Et of the extract 1-2 obtained in Example 1-1 A low-calorie yogurt beverage was prepared under the same conditions as in the above Example 4-A-27 except that the extract 〇.5Kg was substituted for the equol-containing soybean hypocotyl fermentate. 86 200906315 Example 4-A-29 Strawberry Milk Beverage A strawberry milk drink of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) skim milk powder 2.80 5 whole milk powder 3.00 sugar 12.00 emulsifier 0.08 colorant 0.05 spice (strawberry spice) 0.10 1〇 丞_ residual

合計 100.00L 實施例4-A-30 草莓牛奶飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 15 物以外，以與前述實施例4-A-29相同之條件製造草莓牛奶 飲料。 實施例4-A-31 脫脂加工乳 製造下述組成之脫脂加工乳。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.40(Kg) 20 脫脂奶粉 12.00 卵鈣 1.00 乳化劑 0.08 香料 0.10 ^_殘餘部分 87 200906315Total 100.00L Example 4-A-30 Strawberry Milk Beverage In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean embryo is replaced. A strawberry milk drink was produced under the same conditions as in the above Example 4-A-29 except for the shaft fermentation. Example 4-A-31 Degreased milk The degreased milk of the following composition was produced. Soybean hypocotyl ferment containing equol (Reference 1-1) 0.40 (Kg) 20 Skim milk powder 12.00 Egg calcium 1.00 Emulsifier 0.08 Perfume 0.10 ^_Residual part 87 200906315

合計 100.00L 實施例4-A-32 脫脂加工乳 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 5 物以外，以與前述實施例4-A-31相同之條件製造脫脂加工 乳。 實施例4-A-33 霜淇淋蘇打 製造下述組成之霜淇淋蘇打。Total 100.00 L Example 4-A-32 Degreased milk In addition to the solid extract of 萃取.〇5Kg in combination with the extract of 1-2 obtained using 75Et in Example 1-1, the soybean embryo containing equol was replaced. Degreased milk was produced under the same conditions as in the above Example 4-A-31 except for the shaft fermentation. Example 4-A-33 Frosted soda The following composition of cream cream soda was produced.

含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.20(Kg) 10 脫脂加糖練乳 3.00 檸檬酸(結晶） 0.04 砂糖 15.00 著色劑 0.80 香料 0.10 15 水 殘餘部分 合計 實施例4-A-34 霜淇淋，蔽打 40.00L 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇25Kg來取代含雌馬酚之大豆胚軸發 20 酵物以外，以與前述實施例4-A-33相同之條件製造霜淇淋 蘇打。 實施例4-A-35 抹茶牛奶飲料 製造下述組成之抹茶牛奶飲料。 含雌馬紛之大豆胚軸發酵物（參考例1-2) 0.40(Kg) 88 200906315 脫脂奶粉 3.50 牛奶 10.00 乳化劑 0.70 砂糖 8.00 5 ^_殘餘部分Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-1) 0.20 (Kg) 10 Degreased sugared milk 3.00 Citric acid (crystal) 0.04 Sugar 15.00 Colorant 0.80 Perfume 0.10 15 Water residue total Example 4-A- 34 cream, cover 40.00L In addition to the solid extract of 萃取.〇25Kg in the use of 75Et of extract 1-2 obtained in Example 1-1, replace the equol-containing soybean hypocotyl hair 20 In the same manner as in the above Example 4-A-33, frosted soda was produced. Example 4-A-35 Matcha Milk Beverage A matcha milk drink of the following composition was produced. Soybean hypocotyl fermented product containing female horses (Reference 1-2) 0.40 (Kg) 88 200906315 Skim milk powder 3.50 Milk 10.00 Emulsifier 0.70 Sugar 8.00 5 ^_Residual part

合計 100.00L 實施例4-A-36 抹茶牛乳飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 10 物以外，以與前述實施例4-A-35相同之條件製造抹茶牛奶 飲料。 實施例4-A-37 純紅茶(straight tea) 製造下述組成之純紅茶。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 0.40(Kg) 15 大吉嶺紅茶40倍萃取液 20.00 砂糖 5.00 香料 0.05 ^_殘餘部分Total 100.00L Example 4-A-36 Matcha Milk Beverage In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean embryo is replaced. A matcha milk beverage was produced under the same conditions as in the above Example 4-A-35 except for the shaft fermentation. Example 4-A-37 Straight tea A pure black tea of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 0.40 (Kg) 15 Darjeeling black tea 40 times extract 20.00 Sugar 5.00 Spice 0.05 ^_ Residual part

合計 100.00L 20 實施例4-A-38 純紅茶 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-37相同之條件製造純紅茶。 實施例4-A-39 蘋果汁飲料 89 200906315 製造下述組成之蘋果汁飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 〇.40(Kg) 5倍濃縮蘋果透明果汁 2.20 寡糖 1.00 5 還原麥芽糖 1.50 維生素C 0.05 檸檬酸 0.10 水溶性食物纖維 8.00 水 殘餘部分 10 合計 100.00L 實施例4-A-40 葙果汁飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-39相同之條件製造蘋果汁飲 15 料。 實施例4-A-41 清涼飲料 製造下述組成之清涼飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 果糖 3.70 20 果糖葡萄糖液 8.00 砂糖 0.30 DL-蘋果酸 0.07 檸檬酸鈉 0.03 乳化劑 0.052 90 200906315 香料 0.22 ^_殘餘部分Total 100.00 L 20 Example 4-A-38 Pure black tea In addition to the solid extract of 萃取.〇5Kg, which is used in the preparation of extract 1-2 obtained by using 75Et in Example 1-1, the soybean embryo containing equol is replaced. Pure black tea was produced under the same conditions as in the above Example 4-A-37 except for the shaft ferment. Example 4-A-39 Apple Juice Beverage 89 200906315 An apple juice beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 〇.40 (Kg) 5 times concentrated apple transparent juice 2.20 oligosaccharide 1.00 5 reduced maltose 1.50 vitamin C 0.05 citric acid 0.10 water soluble dietary fiber 8.00 water residue Part 10 Total 100.00 L Example 4-A-40 葙 Fruit Juice Beverage In addition to the solid extract of 萃取.〇5Kg used in the extraction of 1-2 5% of the extract 7.5 obtained in Example 1-1, the equol is substituted. An apple juice drink 15 was produced under the same conditions as in the above Example 4-A-39 except for the soybean hypocotyl ferment. Example 4-A-41 Refreshing Beverage A refreshing beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) fructose 3.70 20 fructose glucose solution 8.00 Sugar 0.30 DL-malic acid 0.07 Sodium citrate 0.03 Emulsifier 0.052 90 200906315 Perfume 0.22 ^_Residual part

合計 100.00L 實施例4-A-42 清涼飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-41相同之條件製造清涼飲料。 實施例4-A-43 柑橘果汁飲料 製造下述組成之柑橘果汁飲料。 10 15 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 果糖葡萄糖液 2.50 甜菜寡糖 0.028 砂糖 4.00 柑橘濃縮果汁 4.40 檸檬酸 0.028 檸檬酸鈉 0.003 L-抗壞血酸 0.01 0.10 香料 水 殘餘部分Total 100.00 L Example 4-A-42 A refreshing beverage In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean hypocotyl is replaced. A refreshing beverage was produced under the same conditions as in the above Example 4-A-41 except for the fermented product. Example 4-A-43 Citrus Juice Beverage A citrus juice drink of the following composition was produced. 10 15 Soybean hypocotyl ferment containing equol (Reference 1-3) 0.40 (Kg) fructose glucose solution 2.50 beet oligosaccharide 0.028 sugar 4.00 Citrus juice concentrate 4.40 Citric acid 0.028 Sodium citrate 0.003 L-ascorbic acid 0.01 0.10 Perfume Water residue

20 合計 100.00L 實施例4-A-44 柑橘果汁飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-43相同之條件製造柑橘果汁 91 200906315 飲料。 實施例4-A-45 蘋果汁飲料 製造下述組成之蘋果汁飲料。20 Total 100.00L Example 4-A-44 Citrus Fruit Juice Beverage In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, it is used to replace equol-containing soybeans. A citrus juice 91 200906315 beverage was produced under the same conditions as in the above Example 4-A-43 except for the hypocotyl ferment. Example 4-A-45 Apple Juice Beverage An apple juice beverage of the following composition was produced.

含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 5 蘋果濃縮果汁 4.40 砂糖 8.00 擰檬酸 0.15 香料 0.12 水 殘餘部分 10 合計 100.00L 實施例4-A-46 蘋果汁飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-45相同之條件製造蘋果汁飲 15 料。 實施例4-A-47 蔬果汁 製造下述組成之蔬果汁。Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) 5 Apple juice concentrate 4.40 Sugar 8.00 Lemon acid 0.15 Perfume 0.12 Water residue 10 Total 100.00 L Example 4-A-46 Apple juice The beverage was replaced with the equol-containing soybean hypocotyl fermentate in addition to the solid extract of 萃取.〇5Kg obtained by using 75Et of the extract 1-2 obtained in Example 1-1, in addition to the above-mentioned Example 4- A-45 is made under the same conditions as apple juice. Example 4-A-47 Vegetable Juice A vegetable juice of the following composition was produced.

含雌馬酚之大豆胚軸發酵物（參考例1-2) 0.40(Kg) 蔬果汁 20.00 20 水 殘餘部分 合計 實施例4-A-46 葙果汁 100.00L 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 92 200906315 物以外，以與前述實施例4_A_47相同之條件製造蔬果汁。 复殖1 歹J4-A-47運動飲料 製造下述組成之運動飲料。Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 0.40 (Kg) Vegetable juice 20.00 20 Water residue total Example 4-A-46 Cellulose juice 100.00 L In addition to the use of 75Et in Example 1-1 The obtained fruit juice 1-2 was dried under the same conditions as in the above Example 4_A_47 except that the obtained extract 1-2 dried solid extract 〇 5 Kg was used instead of equol-containing soybean hypocotyl fermentation 92 200906315. Recombination 1 歹J4-A-47 sports drink The following sports drink was made.

1-2) 〇.40(Kg) 1.00 0.25 0.125 0.05 0.50 12.00 殘餘部分 100.00L 含雌馬酚之大豆胚軸發酵物（參考例 乳性礦物質 综合維生素 檸檬酸 L-抗壞血酸鈉 擰檬果汁 砂糖 ^___ ---~~~-___ 合計 例4-A-4S 運動飲料 除了配合實施中使用75Et獲得之萃取液12乾洞 15後的固態狀萃取物0.05Kg來取代含雄馬酚之大豆胚軸發酵 物以外，以與前述實施例4切相同之條件製造運動飲料。 製造下述組成之烏龍茶。 20 含雌馬酚之大豆胚軸發酵物（參考例^ 3) 烏龍茶萃取 L-抗壤企酸納 香料 0.40(Kg) 3.000.01 0.20 合計 ---------殘餘部分1-2) 〇.40(Kg) 1.00 0.25 0.125 0.05 0.50 12.00 Residual part 100.00L equol-containing soybean hypocotyl ferment (Reference example milk minerals comprehensive vitamin citrate L-ascorbate screw lemon juice sugar ^ ___ ---~~~-___ Total Example 4-A-4S Sports Beverage In addition to the 0.05Kg of solid extract after the use of 75Et of the extract 12 roots 15 to replace the soy embryonic axis containing androgen In addition to the fermented product, a sports drink was produced under the same conditions as in the above Example 4. The oolong tea of the following composition was produced. 20 Soybean hypocotyl fermented product containing equol (Reference Example ^ 3) Oolong tea extract L-anti-acidic acid Nano-flavor 0.40 (Kg) 3.000.01 0.20 Total --------- residual

100.00L 93 200906315 實施例4-A-50 烏龍茶 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-49相同之條件製造烏龍茶。 5 f施例4-A-51 檸檬茶 製造下述組成之檸檬茶。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 紅茶萃取 8.00 粉末還原麥芽糖 3.00 10 砂糖 3.00 檸檬果汁 0.50 L-抗壞血酸 0.02 香料 0.10 ^_殘餘部分100.00L 93 200906315 Example 4-A-50 Oolong tea In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean hypocotyl is replaced. Oolong tea was produced under the same conditions as in the above Example 4-A-49 except for the ferment. 5 f Example 4-A-51 Lemon Tea A lemon tea of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Black tea extract 8.00 Powder-reduced maltose 3.00 10 Sugar 3.00 Lemon juice 0.50 L-ascorbic acid 0.02 Fragrance 0.10 ^_Residual

15 合計 100.00L 實施例4-A-52 檸檬茶 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-51相同之條件製造檸檬茶。 20 實施例4-A-53 奶茶 製造下述組成之奶茶。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 牛奶 7.00 全脂奶粉 0.25 94 200906315 砂糖 4.00 紅茶萃取 35.00 乳化劑 0.05 香料 0.10 水 殘餘部分 合計 100.00L 實施例4-A-54 奶茶 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚轴發酵 10 物以外，以與前述實施例4-A-53相同之條件製造奶茶。 實施例4-A-55 咖啡飲料 製造下述組成之咖啡飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.40(Kg) 咖啡萃取 30.00 15 牛奶 25.00 乳化劑 0.10 碳酸氫鈉 0.12 甜菊葉 0.01 砂糖 3.00 20 L-鼠李糖 0.01 香料 0.05 ^_殘餘部分15 Total 100.00L Example 4-A-52 Lemon Tea In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of extract 1-2, the equol-containing soybean embryo is replaced. Lemon tea was produced in the same manner as in the above Example 4-A-51 except for the shaft ferment. 20 Example 4-A-53 Milk Tea A milk tea of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Milk 7.00 Whole milk powder 0.25 94 200906315 Sugar 4.00 Black tea extract 35.00 Emulsifier 0.05 Fragrance 0.10 Water residue total 100.00L Example 4-A -54 The milk tea was replaced with the equol-containing soybean hypocotyl fermentation 10 in combination with 0.05Kg of the solid extract obtained by using 75E of the extract 1-2 obtained in the Example 以, in combination with the above Example 4-A. -53 made milk tea under the same conditions. Example 4-A-55 Coffee Beverage A coffee beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference 1-1) 0.40 (Kg) Coffee extract 30.00 15 Milk 25.00 Emulsifier 0.10 Sodium hydrogencarbonate 0.12 Stevia leaves 0.01 Sugar 3.00 20 L-rhamnose 0.01 Spice 0.05 ^_ Residue

合計 100.00L 實施例4-A-56 咖啡飲料 95 200906315 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-55相同之條件製造咖啡飲料。 實施例4-A-57 可可牛奶飲料 5 製造下述組成之可可牛奶飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.40(Kg) 牛奶 10.00 脫脂奶粉 3.50 5.50 0.40 1.00 果糖 10 砂糖 可可粉 乳化劑 香料 ^_ 0.45 0.08 殘餘部分Total 100.00L Example 4-A-56 Coffee Beverage 95 200906315 In addition to the solid extract of 萃取.〇5Kg after the dry extract of 1-2 obtained in 75 lb of the extract obtained in Example 1-1, the equol-containing soybean was replaced. A coffee beverage was produced under the same conditions as in the above Example 4-A-55 except for the hypocotyl ferment. Example 4-A-57 Cocoa Milk Beverage 5 A cocoa milk drink of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference 1-1) 0.40 (Kg) Milk 10.00 Skim milk powder 3.50 5.50 0.40 1.00 Fructose 10 Sugar Cocoa powder Emulsifier Spices ^_ 0.45 0.08 Residue

15 合計 100.00L 實施例4-A-58 可可牛奶飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-57相同之條件製造可可牛奶 20 飲料。 實施例4-A-59 抹茶牛奶飲料 製造下述組成之抹茶牛奶飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 0.40(Kg) 牛奶 10.00 96 200906315 脫脂奶粉 3.50 果糖 5.80 砂糖 0.40 抹茶 0.90 乳化劑 0.48 香料 0.27 著色劑 0.03 水 殘餘部分15 Total 100.00L Example 4-A-58 Cocoa Milk Beverage In addition to the solid extract of 萃取.〇5Kg used in the extraction of 1-2 5% of the extract 7.5 obtained in Example 1-1, the equol-containing soybean was replaced. In addition to the hypocotyl fermentate, a cocoa milk 20 beverage was produced under the same conditions as in the above-mentioned Example 4-A-57. Example 4-A-59 Matcha Milk Beverage A matcha milk drink of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference 1-1) 0.40 (Kg) Milk 10.00 96 200906315 Skim milk powder 3.50 Fructose 5.80 Sugar 0.40 Matcha 0.90 Emulsifier 0.48 Perfume 0.27 Colorant 0.03 Water Residual

合計 100.00L 10 實施例4-A-60 抹茶牛奶飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-59相同之條件製造抹茶牛奶 飲料。 15 實施例4-A-61 酸乳飲料 製造下列組成之酸乳飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 脫脂奶粉 1.50 果糖 2.50 20 砂糖 0.50 草莓濃縮果汁 0.22 擰檬酸鈉 0.32 乳化劑 0.40 香料 0.12 97 200906315 ^_殘餘部分Total 100.00 L 10 Example 4-A-60 Matcha Milk Beverage In addition to the solid extract of 萃取.〇5Kg, which is used in Example 1-1 using 75Et of the extract 1-2, the estrone-containing soybean is replaced. A matcha milk beverage was prepared under the same conditions as in the above Example 4-A-59 except for the hypocotyl fermentate. 15 Example 4-A-61 Yogurt Beverage A yogurt drink of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) skim milk powder 1.50 fructose 2.50 20 sugar 0.50 strawberry juice concentrate 0.22 sodium citrate 0.32 emulsifier 0.40 fragrance 0.12 97 200906315 ^_

合計 100.00L 實施例4-A-62 酸乳飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 5 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-61相同之條件製造酸乳飲料。 實施例4-A-63 含維生素飲料 製造下述組成之含維生素飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 10 砂糖 24.00 檸檬酸 0.20 L-抗壞血酸 0.30 綜合維生素 0.08 香料 0.10 15 碳酸水_殘餘部分Total 100.00 L Example 4-A-62 Yogurt Beverage In addition to the 0.05 Kg of solid extract after extracting 1-2 dry extract 5 obtained using 75Et in Example 1-1, the equol-containing soybean hypocotyl was replaced. A yogurt drink was produced under the same conditions as in the above Example 4-A-61 except for the ferment. Example 4-A-63 Vitamin-containing beverage A vitamin-containing beverage having the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) 10 Sugar 24.00 Citric acid 0.20 L-ascorbic acid 0.30 Comprehensive vitamin 0.08 Perfume 0.10 15 Carbonated water _ residual

合計 100.00L 實施例4-A-64 含維生素飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 20 物以外，以與前述實施例4-A-63相同之條件製造含維生素 飲料。 實施例4-A-65 紅豆湯 製造下列組成之紅豆湯。 含雌馬盼之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 98 200906315 生紅豆 10.00 糖醇 8.50 砂糖 24.00 食鹽 0.10 乳化劑 0.80 香料 0.10 水 殘餘部分Total 100.00 L Example 4-A-64 Vitamin-containing beverage In addition to the solid extract of 萃取.〇5Kg in combination with the extract of 1-2 obtained in Example 1-1 using 75Et, the soybean embryo containing equol was replaced. A vitamin-containing beverage was produced under the same conditions as in the above Example 4-A-63 except for the shaft fermentation. Example 4-A-65 Red Bean Soup A red bean soup of the following composition was produced. Soybean hypocotyl ferment containing ergot (Reference Example 1-3) 0.40 (Kg) 98 200906315 Raw red beans 10.00 Sugar alcohol 8.50 Sugar 24.00 Salt 0.10 Emulsifier 0.80 Perfume 0.10 Water Residual

合計 100.00L 實施例4-A-66 紅豆湯 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-65相同之條件製造紅豆湯。 實施例4-A-67 綠荃 製造下述組成之綠茶。 15 含雌馬酚之大豆胚轴發酵物（參考例1-1) 〇.40(Kg) 綠茶萃取 3.00 L-抗壞血酸納 0.01 香料 0.20 水 殘餘部分 20 合計 100.00L 實施例4-A-68 綠茶 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-67相同之條件製造綠茶。 99 200906315 實施例4-A-69 柑橘果汁粉末飲料 製造下述組成之柑橘果汁粉末飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 〇-4〇(Kg) 果糖葡萄糖液糖 2.50 5 甜菜寡糖 0.028 砂糖 4.00 柑橘粉末果汁 2.00 檸檬酸 0.028 檸檬酸鈉 0.003 10 L-抗壞血酸 0.01 香料 0.10 水 殘餘部分A total of 100.00 L of Example 4-A-66 Red Bean Soup 10 In addition to the solid extract of 萃取.〇5Kg used in the extraction of 1-2 5% of the extract 1-2 obtained in Example 1-1, the soybean embryo containing equol was replaced. In addition to the shaft ferment, red bean soup was produced under the same conditions as in the above Example 4-A-65. Example 4-A-67 Green 荃 Green tea of the following composition was produced. 15 Soybean hypocotyl ferment containing equol (Reference 1-1) 〇.40 (Kg) Green tea extract 3.00 L-ascorbate 0.01 Fragrance 0.20 Water residue 20 Total 100.00 L Example 4-A-68 Green tea addition The solid extract of the extract 1-2 obtained by using 75Et in Example 1-1 was used in place of the equol-containing soybean hypocotyl fermentate, in addition to the above-mentioned Example 4-A- Green tea was produced under the same conditions of 67. 99 200906315 Example 4-A-69 Citrus juice powder beverage A citrus juice powder beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-2) 〇-4〇(Kg) fructose glucose liquid sugar 2.50 5 beet oligosaccharide 0.028 sugar 4.00 citrus powder juice 2.00 citric acid 0.028 sodium citrate 0.003 10 L- Ascorbic acid 0.01 Fragrance 0.10 Water Residue

合計 100.00L 實施例4-A-70 柑橘果汁粉末飲料 15 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-69相同之條件製造柑橘果汁 粉末飲料。 實施例4-A-71 胺基酸飲料 20 製造下述組成之胺基酸飲料。 含雌馬酚之大豆胚轴發酵物（參考例1-3) 〇.40(Kg) 砂糖 15.00 果糖 15.00 檸檬酸 4.00 100 200906315Total 100.00 L Example 4-A-70 Citrus Fruit Juice Powdered Drink 15 In addition to the solid extract of 萃取.〇5Kg, which was used in Example 1-1 using 75Et of the extract 1-2. A citrus juice powder beverage was produced under the same conditions as in the above Example 4-A-69, except for the soybean hypocotyl ferment. Example 4-A-71 Amino Acid Beverage 20 An amino acid beverage of the following composition was produced. Soybean hypocotyl ferment containing equol (Reference 1-3) 〇.40(Kg) Sugar 15.00 Fructose 15.00 Citric acid 4.00 100 200906315

檸檬酸鈉 1.00 L-顯胺酸 2.00 L-白胺酸 4.00 L-異白胺酸 2.00 L-精胺酸 2.00 香料 0.10 水 殘餘部分 合計 100.00 L f施例4-A-72 胺基酸飲料 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-71相同之條件製造胺基酸飲 料。 實施例4-A-73 豆乳飲料 15 製造下述組成之豆乳飲料。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(重量份） 豆乳_100.00。 實施例4-A-74 豆乳飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-A-73相同之條件製造豆乳飲料。 實施例4-A-75 營養飲料 製造下述組成之營養飲料。 含雌馬酚之大豆胚轴發酵物（參考例1-3) 0.40(Kg) 101 200906315 1.00 0.02 0.005 0.005 0.005 0.05 15.00 15.00 0.10 0.10 殘餘部分 10 牛續酸 菸酸醯胺 維生素B1 維生素B2 維生素B8 咖啡因 砂糖 果糖 檸檬酸 香料 ^_Sodium citrate 1.00 L-leucine 2.00 L-leucine 4.00 L-isoleucine 2.00 L-arginine 2.00 Fragrance 0.10 Water residue total 100.00 L f Example 4-A-72 Amino acid beverage 10 In addition to the above-mentioned Example 4-A, in addition to the solid extract of 萃取.〇5Kg in which the extract obtained by using 75Et in Example 1-1 was used to replace the equol-containing soybean hypocotyl fermentate, An amino acid beverage was produced under the same conditions as -71. Example 4-A-73 Soymilk Beverage 15 A soymilk drink of the following composition was produced. Soybean hypocotyl ferment containing equol (Reference Example 1-3) 0.40 (parts by weight) Soymilk _100.00. Example 4-A-74 Soymilk Beverage In addition to the 0.05 Kg of the solid extract after extracting 1-2 of the extract 1-2 obtained in 75 Et in Example 1-1, the equol-containing soybean hypocotyl fermentate was replaced. Soymilk beverage was produced under the same conditions as in the above Example 4-A-73. Example 4-A-75 Nutritional Beverage A nutritional beverage of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) 101 200906315 1.00 0.02 0.005 0.005 0.005 0.05 15.00 15.00 0.10 0.10 Residue 10 Bovine acid nicotinate valeramine Vitamin B1 Vitamin B2 Vitamin B8 Coffee Sand candy sugar citric spice ^_

合計 100.00L 實施例4-A-76 胺基酸飲料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 15 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-A-71相同之條件製造營養飲料。 實施例4-B 補充品 以下，就本發明補充品之製造例列舉具體例。 實施例4-B-1 軟膠囊 20 使用含雌馬酚之大豆胚軸發酵物（參考例1-3)、明膠、 甘油、檸檬酸、大豆油、蜜蠘、卵鱗脂、β胡蘿蔔素及水， 依常法製造軟膠囊。 實施例4-Β-2 軟膠囊 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 102 200906315 後的固態狀萃取物來取代含雌馬酚之大豆胚軸發酵物以 外，以與前述實施例4-B-1相同之條件製造軟膠囊。 實施例4-C 奶油狀食品 以下就本發明之奶油狀食品製造例列舉具體例。 5 實施例4-C-1 泡沫奶油 製造下述組成之泡沫奶油。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(g) 椰子油（mp35°C) 27.00 甘油脂肪酸酯 0.10 10 脫脂奶粉 3.30 蔗糖素 0.012 還原澱粉糖化物 25.00 卵磷脂 0.15 乳化劑 0.70 15 甲基磷酸鈉 0.10 ^_殘餘部分 合計 100.00g 實施例4-C-2 泡沫奶油 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.05g來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-C-1相同之條件製造泡沫奶油。 實施例4-C-3 咖啡奶精 製造下述組成之咖啡奶精。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(g) 103 200906315 純化椰子油 38.00 酷蛋白納 4.50 脫脂奶粉 5.00 卵磷脂 0.05 檸檬酸三鈉 0.20 蔗糖脂肪酸酯 0.40 牛奶香料 0.10 水 殘餘部分 合計 100.00g 10 實施例4-C-4 咖啡奶精 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5g來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-C-3相同之條件製造咖啡奶精。 實施例4-C-5 卡士達奶油 15 製造下述組成之卡士達奶油。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 乳瑪琳 20.00 麥芽糖 5.00 全蛋 3.00 20 玉米澱粉 4.00 加工澱粉 8.00 乳性蛋白 2.00 砂糖 30.00 水 殘餘部分 104 200906315 合計 100.00 實施例4-C-6 卡士達奶油 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5g來取代含雌馬紛之大豆胚軸發酵 5 物以外，以與前述實施例4-C-5相同之條件製造卡士達奶 油。 實施例4-C-7 卡士这奶油 製造下述組成之卡達奶油。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 〇.40(Kg) 10 鹿角菜膠 0.40 明膠 0.50 玉米澱粉 4.00 糖粉 3.00 砂糖 10.00 15 葡萄糖 7.00 脫脂奶粉 4.00 全蛋 3.50 異性化糖 7.00 脫脂加糖練乳 3.00 20 無鹽奶油 8.50 水 50.00 實施例4-C-8 卡士達奶油 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05kg/來取代含雌馬紛之大豆胚軸發酵 105 200906315 物以外，以與前述實施例4-C-7相同之條件製造卡士達奶 油。 甜點 以下就本發明之甜點製造例列舉豆體例。 硬優格 ~ 使上記組成原料均質化後，添加起始優格、啟動優格 並發酵’以製造硬優格。 10 15 含雌馬酚之大豆胚軸發酵物（參考例 1-3) 〇.4〇(Kg) 脱脂奶粉 9.00 無鹽奶油 5.00 砂糖 8.50 膠化劑 0.80 水 殘餘部分 合計 lOO.OOKg 實施例4-D-2 練停格 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05Kg來取代含雌馬酚之大豆胚軸發酵 物以外’以與前述實施例4-D-1相同之條件製造硬優格。 實施例4-D-3 軟優格 20 使下述組成原料均質化後，添加啟動優格使其發酵而 製得軟優格。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(Kg) 脫脂奶粉 9.00 奶油 5.25 106 200906315 砂糖 8.50 刺槐豆膠 0.30 水 殘餘部分 合計 lOO.OOKg 5 實施例4-D-4 軟優格 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-D-3相同之條件製造軟優格。 實施例4-D-5 白桃果凍 10 使用下述成分作為原料，依照果凍之一般製造方法， 製造白桃果凍。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份) 砂糖 14.40 κ-鹿角菜膠 0.80 15 白桃果泥 20.00 ^_殘餘部分 合計 100.00重量份 實施例4-D-6 白桃果凍 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-D-5相同之條件製造白桃果 凍。 實施例4-D-7 葡萄柚果凍 使用下述成分作為原料，依照果凍一般之製造方法製 107 200906315 造葡萄柚果練。 含雌馬酚之大豆胚軸發酵物(參考例1-2) 〇·4〇(重量份） 砂糖 18.00 結冷膠 0.25 5 葡萄柚果汁 20.00 乳酸鈣 0.10 檸檬酸 0.20 香料 0.10 水 殘餘部分 10 合計 100.00重量份 實施例4-D-8 葡葙柚果凌 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚轴 發酵物以外，以與前述實施例4-D-7相同之條件製造葡萄柚 15 果凍。 實施例4-D-9 咖啡;東 使用下述成分作為原料，依照果凍之一般製造方法製 造咖啡凍。 含雌馬酚之大豆胚軸發酵物(參考例1-2) 0.40(重量份) 20 還原麥芽糖 10.00 木醣醇 5.00 κ-鹿角菜膠 0_50 洋菜 0.20 刺槐豆膠 0.20 108 200906315 砂糖 3.00 香料 0.15 ^_殘餘部分 合計 100.00重量份。 5 實施例4-D-10 咖#;東 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇·〇5重量份來取代含雌馬酚之大豆胚轴 發酵物以外，以與前述實施例4-D-9相同之條件製造葡萄柚 果珠。 10 實施例4-D-11 草莓牛奶凍 使用下述成分作為原料，依照果凍一般之製造方法， 製造草莓牛奶果凍。 15 20 含雌馬酚之大豆胚軸發酵物(參考例1-2) 0.40(重量份) 純化椰子油 30.00 乳化劑 0.25 脫脂奶粉 3.30 砂糖 3.50 還原麥芽糖 20.00 乳糖醇 8.00 安定化劑 0.70 木醣醇 5.00 明膠 0.90 草莓果泥 5.00 草莓濃縮果汁 5.00 109 200906315 檸檬酸 0.10 ζκ_殘餘部分 合計 100·00重量份 實施例4-D-12 草莓牛奶凍 5 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-D-11相同之條件製造草莓 牛奶凍。 實施例4-E 點心 10 以下就本發明點心之製造例列舉具體例。 實施例4-E-1 硬糖果 使用下述成分作為原料，依照硬糖果之一般製造方法 來製造硬糖果。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(g) 15 砂糖 70.00 麥芽糖 40.00 水 20.00 實施例4-E-2 硬糖果 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.5g來取代含雌馬酚之大豆胚軸發酵物 以外，以與前述實施例4-E-1相同之條件製造硬糖果。 實施例4-E-3 生薑糖 使用下述成分作為原料，依照一般製糖方法製造生薑 糖。 110 200906315 含雌馬酚之大豆胚軸發酵物（參考例1-1) 4.00(g) 生薑膏 1.50 還原乳糖 80.00 還原麥芽糖 58.00 5 蔗糖素 0.03 香料 0.075 ^_30.00 實施例4-E-4 生薑糖 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 10 後的固態狀萃取物0.5g來取代含雌馬盼之大豆胚軸發酵物 以外，以與前述實施例4-E-3相同之條件製造生薑糖。 實施例4-E-5 草本喉糖 使用下述成分作為原料，依照一般製糖方法製得草本 喉糖。 15 含雌馬酚之大豆胚軸發酵物（參考例1-2) 4.00(g) 綜合藥草萃取 0.80 砂糖 40.00 還原麥芽糖 58.00 歐薄荷香料 0.20 20 焦糖色素 0.20 ^_20.00 實施例4-E-6 草本喉糖 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5g來取代含雌馬盼之大豆胚軸發酵物 111 200906315 以外，以與前述實施例4如相 ^檸檬軟膠糖 同之條件製造草本喉糖。 5 使用下述成分作為原料，依 法，製得檸檬軟膠糖。 a膠狀糖果的-般製造方 含雌馬酚之大豆胚軸發酵物 酸性明膠(25〇布魯姆） 還原麥芽糖 例 1-2) 4.00(g) 7.00 10 4倍濃縮檸檬果汁 檸檬酸(結晶） 蔗糖素 著色劑 香料 100.00 1.25 1.00 0.02 0.02 0.0212,00 15 除了配合實施例W中使㈣Et獲得之萃取液丨_2乾涸 後的固態狀萃取物〇.5g來取代含雌馬盼之大豆胚軸發酵物 以外，以與前述實施例4-E-7相同之條件製造棒樣軟膠橋。 實你例4-E-9 太妃糖 使用下述成分作為原料，依照太妃糖之一 般製造方法 來製造太妃糖。 含雌馬紛之大豆胚軸發酵物（參考例U) 4.00(g) 加糖練乳 45.00 麥穿糖 45.00 植物油脂 3.50 112 200906315 印構脂 0.20 翻糖（fondant) 5.00 香料（太妃糖香料)_0.10 實施例4-E-10 太妃糖 5 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬紛之大豆胚抽發酵物 以外，以與前述實施例4-E-9相同之條件製造太妃糖。 實施例4-E-11 普澤餅 混合下述成分調製成生料，將其置於烤箱烘焙而製出 10 普澤餅。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 4.00(g) 高筋麵粉 100.00 發粉 1.35 無鹽奶油 25.00 15 食鹽 2.00 砂糖 17.50 大豆食物纖維 0.80 蔗糖脂肪酸酯 0.30 香料 0.20 20 水 40.00 實施例4-E-12 普澤餅 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬紛之大豆胚軸發酵物 以外，以與前述實施例4-E-11相同之條件製造普澤餅。 113 200906315 實施例4-E-13 薄荷錠 使用下述成分作為原料，依照錠片之一般製造方法來 製造薄荷鍵。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(g) 5 山梨糖醇 90.00 蔗糖素 0.15 歐薄荷香料 5.00 蔗糖脂肪酸酉旨_!·〇〇 實施例4-E-14 薄荷錠 10 除了配合實施例1-1中使用2H1獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬紛之大豆胚轴發酵物 以外，以與前述實施例4-E-13相同之條件製造薄荷錠。 實施例4-E-15 優格錠 使用下述成分作為原料，依照錠片之一般製造方法製 15 得優格鍵。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 4.00(g) 海藻糖(造粒） 88.80 蔗糖素 〇.〇8 粉末發酵乳 5.00 20 無水檸檬酸 1.00 優格香料 0.20 蔗糖脂肪酸醋_1.50 實施例4-E-16 優格錠 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 114 200906315 後的固態狀萃取物〇.5g來取代含雌馬酚之大豆胚軸發酵物 以外，以與前述實施例4-E-15相同之條件製造優格鍵。 實施例4-E-17 檸檬錠 使用下述成分作為原料，依照錠片之一般製造方法製 5 造棒樣鍵。 含雌馬酚之大豆胚轴發酵物（參考例1-1) 4.00(g) 砂糖(造粒） 89.50 粉末檸檬果汁 2.00 L-抗壞血酸 3.00 10 無水擰檬酸 1.00 蔗糖脂肪酸脂_1.00 實施例4-E-18 檸檬錠 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬盼之大豆胚軸發酵物 15 以外，以與前述實施例4-E-17相同之條件製造擰檬鍵。 實施例4-E-19 薄荷口香糖 使用下述成分作為原料，依照口香糖之一般製造方法 來製造薄荷口香糖。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 4.00(g) 20 口香糖基質 25.00 乳糖醇 83.20 糖醇 5.00 蔗糖素 0.13 甘油 0.50 115 200906315 歐薄荷油 1.50 薄荷香料 0.50 著色劑_0.20 實施例4-E-20 薄荷口香糖 5 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬酚之大豆胚轴發酵物 以外，以與前述實施例4-E-19相同之條件製造薄荷口香糖。 實施例4-E-21 蘋果泡泡糖 使用下述成分作為原料，依照泡泡糖之一般製造方法 10 製得蘋果泡泡糖。 含雌馬酚之大豆胚軸發酵物（參考例1-1) 4.00(g) 泡泡糖基質 30.00 益壽糖(palatinit) 70.00 蔗糖素 0.084 15 甘油 0.50 檸檬酸(結晶） 1.00 蕷果香料_0-80 實施例4-E-22 蘋果泡泡糖 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.5g來取代含雌馬酚之大豆胚軸發酵物 以外，以與前述實施例4-E-21相同之條件製造蘋果泡泡糖。 實施例4-E-23 草莓口香糖 使用下述成分作為原料，依照口香糖一般之製造方法 來製造草莓口香糖。 116 200906315 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(g) 口香糖基質 23.00 糖粉 82.00 麥芽糖 5.00 5 蔗糖素 0.001 檸檬酸(結晶） 1.20 紫萵苣色素 0.20 箪莓香料_0.20 實施例4-E-24 草莓口香糖 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬酚之大豆胚軸發酵物 以外，以與前述實施例4-E-23相同之條件製造草莓口香糖。 實施例4-E-25 巧克力 使用下述成分作為原料，依照巧克力之一般製造方法 15 製得巧克力。 含雌馬酚之大豆胚轴發酵物（參考例1-3) 4.00(g) 可可塊 40.00 可可脂 25.00 還原粉糖 50.00 20 蔗糖素 0.03 卵磷脂 0.40 香草精_0.05 實施例4-E-26 巧克力 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 117 200906315 後的固態狀萃取物〇.5g來取代含雌馬酚之大豆胚軸發酵物 以外，以與前述實施例4-E-25相同之條件製造巧克力。 實施例4-E-27 草莓風味巧克力 使用下述成分作為原料，依照巧克力之一般製造方法 5 製得草莓風味巧克力。 含雌馬酚之大豆胚轴發酵物（參考例1-3) 4.00(g) 可可脂 25.00 硬奶油 7.00 全脂奶粉 27.00 10 砂糖 41.00 草莓香料 0.20 甜菜紅 0.50 蔗糖素 0.001 卵填脂 0.30 15 香草精 0.02 實施例4-E-28 草莓風味巧克力 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5g來取代含雌馬紛之大豆胚轴發酵物 以外，以與前述實施例4-E-27相同之條件製造草莓風味巧 20 克力。 實施例4-E-29 小甜餅 混合下述成分製成生料，使其於烤箱烘焙而製得小甜 餅。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份） 118 200906315 低筋麵粉 48.50 發粉 0.30 無鹽乳瑪琳 24.00 食鹽 0.20 砂糖 12.00 蛋黃 4.70 海藻糖 22.00 香料 0.20 水 40.00 10 實施例4-E-30 小甜餅 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-29相同之條件製造小甜 餅。 15 實施例4-E-31 包子皮 使用下述成分，依照包子皮之一般製造方法而製得包 子皮。 含雌馬酚之大豆胚轴發酵物(參考例1-2) 4.00(重量份) 低筋麵粉 500.00 20 發粉 2.50 三仙膠 1.00 食鹽 8.00 砂糖 30.00 海藻糖 30.00 119 200906315 乾酵母 豬油 水 7.50 15.00 200.00 實施例4-E-32 包子皮 5 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚軸 發酵物以外， 以與前述實施例4-E-31相同之條件製造包子 皮。 實施例4-E-33 多員果派銘 10 使用下述成分製造蘋果派餡。 含雌馬酚之大豆胚軸發酵物(參考例1-1) 0.40(重量份） 蘋果 200.00 麥芽糖 150.00 蔗糖素 0.10 15 棒樣果汁 10.00 胡桃 80.00 葡萄乾 80.00 蘋杲香料 0.20 實施例4-E-34 蘋果派餡 20 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-33相同之條件製造蘋果 派銘。 實施例4-E-35 雪藏蛋糕 120 200906315 混合下述成分調製生料，使其於烤箱烘焙而製得雪藏 蛋糕。 含雌馬酚之大豆胚軸發酵物(參考例1 -1) 0.40(重量份) 100.00 100.00 0.03 0.03 100.00 0.22 120.00 1.50 奶油 5 還原麥芽糖 蔗糖素 甜菊葉 全蛋 香料 10 低筋麵粉 膨脹劑_ 實施例4-E-36 雪藏蛋糕 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚轴 15 發酵物以外，以與前述實施例4-E-35相同之條件製造雪藏 蛋糕。 實施例4-E-37 蕨餅 使用下述成分製造蕨餅。 含雌馬酚之大豆胚轴發酵物(參考例1-1) 0.40(重量份) 20 米澱粉 20.00 甘藷澱粉 40.00 砂糖 8.00 ZK_殘餘部分 100.00重量份 合計 121 200906315 實施例4-E-38 蕨餅 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇·〇5重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-Ε-37相同之條件製造蕨餅。 f施例4-Ε-39 豆菓子 使用下述成分製造豆菓子。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 8.00(重量份） 寒梅粉 7.00 砂糖 53.00 10 黃旦粉 34.00 膨脹劑 適量 香料 適量 甘味料 適量 合計 15 實施例4-E-40 豆菓子 100.00重量份 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.75重量份來取代含雌馬酚之大豆胚轴 發酵物以外，以與前述實施例4-E-39相同之條件製造豆菓 子。 20 實施例4-E-41 豆菓子 使用下述成分製造豆菓子。 含雌馬酚之大豆胚轴發酵物(參考例1-2) 12.00(重量份) 糯米粉 13.00 砂糖 45.00 122 200906315 黃豆粉 15.00 抹茶 15.00 膨脹劑 適量 香料 適量 甘味料 適量 合計 100.00重量份 實施例4-E-42 豆菓子 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物1.5重量份來取代含雌馬酚之大豆胚轴 10 發酵物以外，以與前述實施例4-E-41相同之條件製造豆菓 子。 實施例4-E-43 豆菓子 使用下述成分製造豆菓子。 含雌馬酚之大豆胚軸發酵物(參考例1-1) 18.00(重量份) 15 寒梅粉 15.00 砂糖 52.00 黃豆粉 15.00 抹茶 12.00 膨脹劑 適量 20 香料 適量 甘味料 適量 合計 100.00重量份 實施例4-E-44 豆菜子 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 123 200906315 後的固態狀萃取物2.25重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-43相同之條件製造豆菓 子0 實施例4-E-45 豆菓子 5 使用下述成分製造豆菓子。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 8.00(重量份) 澱粉 20.00 砂糖 32.00 黃丑粉 10.00 10 黑芝麻 20.00 膨脹劑 適量 香料 適量 甘味料 適量 合計 100.00重量份 15 實施例4-E-46 豆菓子 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.75重量份來取代含雌馬酚之大豆胚轴 發酵物以外，以與前述實施例4-E-45相同之條件製造豆菓 子。 20 實施例4-E-47 生麩菓子 使用下述成分製造生麩菓子。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 10.00(重量份） 麩質（gluten) 糯米粉 20.00 15.00 124 200906315 黃豆粉 適量 乳化劑 適量 適量 山梨糖醇 20.00 5 巧克力 30.00 實施例4-E-48 生黏莫手 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物1.25重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-47相同之條件製造生麩 10 菓子。 實施例4-E-49 鬆餅（澧咮帮） 使用下述成分，依照鬆餅之一般製造方法製得鬆餅（濃 味型）。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份) 15 麵粉 100.00 砂糖 2.00 油脂 3.00 全蛋 3.00 脫脂奶粉 2.00 20 礙酸氳納 0.30 牛奶 50.00 水 100.00 實施例4-E-50 鬆餅(濃味型) 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 125 200906315 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-49相同之條件製造鬆餅 (濃味型）。 實施例4-E-51 鬆餅(原味） 5 使用下述成分，依照鬆餅之一般製造方法製得鬆餅(原 味）。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份） 麵粉 100.00 油脂 2.50 0.30 0.40 0.30 0.05 10 全蛋 碳酸銨 碳酸氫納 卵填脂 水 150.00 15 實施例4-E-52 鬆餅(原味） 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-51相同之條件製造鬆餅 (原味）。 20 實施例4-E-53 起司硬餅乾 使用下述成分，依照一般製造方法製得起司硬餅乾。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份） 麵粉 100.00 油脂 9.00 126 200906315 麥芽萃取 1.30 碳_酸氫鈉 0.80 香辛料 0.10 起司粉 13.00 砂糖 80.00 食鹽 0.90 碳酸銨 0.80 卵磷脂 0.05 水 33.00 10 實施例4-E-54 起司硬餅乾 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酌·之大豆胚軸 發酵物以外，以與前述實施例4-E-53相同之條件製造起司 硬餅乾。 15 實施例4-E-55 羊羹 使用下述成分，依照一般製造方法製得羊羹。 含雌馬酚之大豆胚轴發酵物(參考例1-3) 4.00(重量份） 7.50 320.00 820.00 50.00 300.00 洋菜 砂糖 20 紅豆 麥芽糖 ^_ 實施例4-E-56 羊羹 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 127 200906315 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-55相同之條件製造羊羹。 實施例4-E-57 軟小甜餅 使用下述成分，依照一般製造方法製得軟小甜餅。 5 含雌馬酚之大豆胚軸發酵物(參考例1-3) 4.00(重量份) 麵粉 200.00 奶油 150.00 砂糖 80.00 全蛋 150.00 10 實施例4-E-58 軟小甜餅 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-57相同之條件製造軟小 甜餅。 15 實施例4-E-59 仙貝 使用下述成分，依照一般製造方法製得仙貝。 含雌馬酚之大豆胚軸發酵物(參考例1 -3) 0.40(重量份） 梗米 100.00 熱水_適量 20 實施例4-E-60 仙貝 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚轴 發酵物以外，以與前述實施例4-E-59相同之條件製造仙貝。 實施例4-E-61 洲濱餅 128 200906315 使用下述成分，依一般製造方法製得洲濱餅。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 4.00(重量份) 800.00 400.00 130.00 180.00 精白糖 黃豆粉 麥芽糖 ^_ 實施例4-E-62 洲濱餅 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚軸 10 發酵物以外，以與前述實施例4-E-61相同之條件製造洲濱 餅。 實施例4-E-63 千菓子 使用下述成分，依照一般製造方法製造千菓子。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 4.00(重量份) 500.00 25.00 75.00 15 和三盆糖 Shitori 寒梅粉 實施例4-E-64 千菓子 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.5重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-E-63相同之條件製造千菓 子。 實施例4-E-65 水果軟膠糖 使用下述成分，依照一般製造方法製造水果軟膠糖。 129 200906315 含雌馬酚之大豆胚軸發酵物(參考例 砂糖 麥芽糖 明膠 4·〇〇(重量份) 30.00 5〇.〇〇 7.〇〇 水果汁 檸檬酸 香料 5.00 1.5〇 實施例4-Ε-66 水果軟膠糖A total of 100.00 L of Example 4-A-76 Amino Acid Beverage In addition to the solid extract of 0.050 g of the extract 1-2 obtained in Example 1-1, the 0.05 g of the solid extract was used to replace the equol-containing soybean embryo. A nutrient beverage was produced under the same conditions as in the above Example 4-A-71 except for the shaft ferment. Example 4-B Supplementary Examples Hereinafter, specific examples of the production examples of the supplement of the present invention will be given. Example 4-B-1 Soft Capsule 20 Use equol-containing soybean hypocotyl ferment (Reference Example 1-3), gelatin, glycerin, citric acid, soybean oil, candied fruit, egg yolk fat, beta carotene and Water, soft capsules are made according to the usual method. Example 4 - Β-2 Soft capsule In addition to the solid extract obtained by using 75Et of the extract 1-2 dried 涸102 200906315 obtained in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, Soft capsules were produced under the same conditions as in the above Example 4-B-1. Example 4-C Creamy Food The following is a specific example of the creamy food preparation of the present invention. 5 Example 4-C-1 Foam cream A foamed cream of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (g) Coconut oil (mp 35 ° C) 27.00 Glycerol fatty acid ester 0.10 10 Skim milk powder 3.30 Sucralose 0.012 Reduced starch saccharide 25.00 Lecithin 0.15 Emulsified Agent 0.70 15 sodium methyl phosphate 0.10 ^_residual total 100.00 g Example 4-C-2 Foam cream In addition to the solid extract obtained in accordance with the use of 75Et of extract 1-2 dried hydrazine 20 in Example 1-1, 0.05 A foamed cream was produced under the same conditions as in the above Example 4-C-1 except that g of the equol-containing soybean hypocotyl fermentate was replaced. Example 4-C-3 Coffee Creamer A coffee creamer of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (g) 103 200906315 Purified coconut oil 38.00 Cool protein sodium 4.50 Skim milk powder 5.00 Lecithin 0.05 Trisodium citrate 0.20 Sucrose fatty acid ester 0.40 Milk flavor 0.10 Total water residue 100.00 g 10 Example 4-C-4 The coffee creamer was replaced with the solid extract of the extract 1-2 obtained by using 75Et in Example 1-1, and the solid extract was replaced by 5 g to replace the female horse. A coffee creamer was produced under the same conditions as in the above Example 4-C-3 except for the phenolic soybean hypocotyl fermentate. Example 4-C-5 Custard Cream 15 The following composition of kastida cream was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Milk Marlin 20.00 Maltose 5.00 Whole Egg 3.00 20 Corn Starch 4.00 Processed Starch 8.00 Milk Protein 2.00 Sugar 30.00 Water Residue 104 200906315 Total 100.00 Example 4-C-6 Custard Butter In addition to the solid extract of 萃取.〇5g, which is used in Example 1-1 using 75Et of the extract 1-2, the soybean extract is substituted for the fermentation of soybean-containing hypocotyls. In addition to the materials, the kista cream was produced under the same conditions as in the above Example 4-C-5. Example 4-C-7 This cream was made into the cream of the following composition. Soybean hypocotyl ferment containing equol (Reference 1-3) 〇.40(Kg) 10 Carrageenan 0.40 Gelatin 0.50 Corn starch 4.00 Sugar powder 3.00 Sugar 10.00 15 Glucose 7.00 Skim milk powder 4.00 Whole egg 3.50 Heterosexualized sugar 7.00 Degreased and sweetened milk 3.00 20 Unsalted cream 8.50 Water 50.00 Example 4-C-8 Custard cream In addition to the extract of the extract obtained using 75Et in Example 1-1, the dried solid extract 0.05kg/ In addition to the female soy embryo fermentation 105 200906315 containing the female horse, the kista cream was produced under the same conditions as the above-mentioned Example 4-C-7. Desserts The following examples of the manufacture of the dessert of the present invention are given. Hard Yogurt ~ After homogenizing the constituent materials, add the starting yoghurt, start the yoghurt and ferment ‘ to make a hard yoghurt. 10 15 Soybean hypocotyl ferment containing equol (Reference 1-3) 〇.4〇(Kg) Skim milk powder 9.00 Unsalted cream 5.00 Sugar 8.50 Gelling agent 0.80 Water residue total lOO.OOKg Example 4- D-2 is not in addition to the equol-containing soybean hypocotyl fermentate in combination with 0.05Kg of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1. The hard conditions were produced under the same conditions as in Example 4-D-1. Example 4-D-3 Soft Optimum 20 After homogenizing the following constituent materials, a yoghurt was added and fermented to obtain a soft yoghurt. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) skim milk powder 9.00 cream 5.25 106 200906315 sugar 8.50 locust bean gum 0.30 water residue total lOO.OOKg 5 Example 4-D-4 Soft In addition to the solid extract of 萃取.〇5Kg of the extract 1-2 obtained by using 75Et in the embodiment, the yoghurt is replaced with the equol-containing soybean hypocotyl fermentate, in the same manner as the foregoing example 4-D. -3 creates the soft yoghurt under the same conditions. Example 4-D-5 White Peach Jelly 10 The white peach jelly was produced in accordance with the general manufacturing method of the jelly using the following ingredients as raw materials. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) Sugar 14.40 κ-carrageenan 0.80 15 White peach puree 20.00 ^_ Total residue 100.00 parts by weight Example 4-D-6 The peach jelly is replaced with the equol-containing soybean hypocotyl fermentate in addition to 0.05 parts by weight of the solid extract obtained by using 75Et of the extract 1-2 dried hydrazine 20 obtained in Example 1-1, in combination with the foregoing Example 4 White peach jelly was produced under the same conditions as -D-5. Example 4-D-7 Grapefruit Jelly The following ingredients were used as raw materials, and were prepared according to the general manufacturing method of jelly. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-2) 〇·4〇 (parts by weight) Sugar 18.00 Gelatin 0.25 5 Grapefruit juice 20.00 Calcium lactate 0.10 Citric acid 0.20 Perfume 0.10 Water residue 10 Total 100.00重量份份例例4-D-8 葙 葙 葙 除了 除了 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 配合 取代 取代 取代 取代 取代Grapefruit 15 jelly was produced under the same conditions as in the above Example 4-D-7 except for the shaft ferment. Example 4-D-9 Coffee; East The following ingredients were used as raw materials to prepare coffee jelly in accordance with the general manufacturing method of jelly. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 0.40 (parts by weight) 20 Reduced maltose 10.00 Xylitol 5.00 κ-carrageenan 0_50 Amaranth 0.20 Locust bean gum 0.20 108 200906315 Sugar 3.00 Spice 0.15 ^ The residual portion was 100.00 parts by weight in total. 5 实施例4-D-10 咖#; East in addition to the use of 75Et of the extract 1-2 obtained in Example 1-1 using the solid extract 〇·〇 5 parts by weight to replace the equol-containing soybean Grapefruit beads were produced in the same conditions as in the above Example 4-D-9 except for the hypocotyl fermentate. 10 Example 4-D-11 Strawberry milk jelly Using the following ingredients as raw materials, strawberry milk jelly was produced according to the general method of manufacturing jelly. 15 20 Soybean hypocotyl ferment containing equol (Reference 1-2) 0.40 (parts by weight) Purified coconut oil 30.00 Emulsifier 0.25 Skim milk powder 3.30 Sugar 3.50 Restored maltose 20.00 Lactitol 8.00 Stabilizer 0.70 Xylitol 5.00 Gelatin 0.90 Grass Berry Mud 5.00 Strawberry Concentrate Juice 5.00 109 200906315 Citric Acid 0.10 ζ κ _ Residual Total 100·00 parts by weight Example 4-D-12 Strawberry Milk Flake 5 In addition to the extract obtained by using 75Et in Example 1-1 Strawberry milk fry was produced under the same conditions as in the above Example 4-D-11, except that 0.05 parts by weight of the dried solid extract was substituted for the equol-containing soybean hypocotyl fermentate. Example 4-E Dim Slim 10 Hereinafter, specific examples of the production of the dim sum of the present invention will be described. Example 4-E-1 Hard candy The hard candy was produced in accordance with the general method for producing a hard candy using the following ingredients as a raw material. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (g) 15 Sugar 70.00 Maltose 40.00 Water 20.00 Example 4-E-2 Hard candy In addition to the extraction obtained by using 75Et in Example 1-1 Hard candy was produced under the same conditions as in the above Example 4-E-1, except that 0.5 g of the solid extract after the liquid 1-2 was dried over 20 to replace the equol-containing soybean hypocotyl fermentate. Example 4-E-3 Ginger Sugar Ginger sugar was produced according to a general sugar-making method using the following components as a raw material. 110 200906315 Soybean hypocotyl ferment containing equol (Reference 1-1) 4.00 (g) Ginger cream 1.50 Reducing lactose 80.00 Reducing maltose 58.00 5 Sucralose 0.03 Perfume 0.075 ^_30.00 Example 4-E-4 The ginger sugar was replaced with 0.5 g of the solid extract after extracting 1-2 dry extract 10 obtained in 75 Et in Example 1-1 to replace the soybean hypocotyl fermented product containing the esculent, with the foregoing Example 4 -E-3 produces ginger sugar under the same conditions. Example 4-E-5 Herbal throat lozenges Herbal throat lozenges were prepared according to the usual sugar-making method using the following ingredients as raw materials. 15 Soybean hypocotyl ferment containing equol (Reference 1-2) 4.00 (g) Integrated herb extract 0.80 Sugar 40.00 Restored maltose 58.00 Spearmint 0.20 20 Caramel 0.20 ^_20.00 Example 4-E- 6 The herb throat lozenge is replaced with 0.5 g of the solid extract obtained by using the extract of 1-2 in the Example 1-1, and the soybean-like hypocotyl fermentate 111 200906315 is replaced by the above-mentioned Example 4: Manufacture of herbal throat lozenges under the same conditions as lemon soft gum. 5 Using the following ingredients as raw materials, lemon soft gum was obtained according to the method. a gelatinous confectionery - the general production of equol containing soybean hypocotyl fermentation fermented acid gelatin (25 〇 Bloom) reducing maltose 1-2) 4.00 (g) 7.00 10 4 times concentrated lemon juice citric acid (crystallization Sucrose sucrose colorant 100.00 1.25 1.00 0.02 0.02 0.0212,00 15 In addition to the solid extract of (2, which is obtained by the extraction of (_2 from the extract obtained by (4) Et in Example W, the soybean hypocotyl containing equin is replaced. A rod-like soft rubber bridge was produced under the same conditions as in the above Example 4-E-7 except for the ferment. Example 4-E-9 Toffee Use the following ingredients as a raw material to make toffee according to one of the toffee manufacturing methods. Soybean hypocotyl fermented product containing female horses (Reference U) 4.00 (g) Sugared milk 45.00 Wheat sugar 45.00 Vegetable fat 3.50 112 200906315 Printing fat 0.20 Fondant 5.00 Spice (toffee spice)_0. 10 Example 4-E-10 Toffee 5 In addition to the solid extract of 萃取.5g after the dry extract of 1-2 using the extract obtained by using 75Et in Example 1-1, the soybean embryo fermentation was replaced by the female horse. Toffee was produced in the same conditions as in the above Example 4-E-9 except for the above. Example 4-E-11 Puer Cake The following ingredients were mixed to prepare a raw meal, which was baked in an oven to prepare a 10 Pus cake. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-1) 4.00 (g) High-gluten flour 100.00 Hair powder 1.35 Unsalted cream 25.00 15 Salt 2.00 Sugar 17.50 Soy food fiber 0.80 Sucrose fatty acid ester 0.30 Perfume 0.20 20 Water 40.00 Example 4-E-12 Puze cake In addition to the solid extract of 萃取.5g after the dry extract of 1-2 of the extract 1-2 obtained in Example 1-1 was used to replace the soybean hypocotyl fermented product containing the equine The pudding cake was produced under the same conditions as in the above Example 4-E-11 except for the above. 113 200906315 Example 4-E-13 Peppermint Ingot The following ingredients were used as raw materials, and a mint bond was produced in accordance with the general production method of the tablet. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (g) 5 Sorbitol 90.00 Sucralose 0.15 Spearmint Spice 5.00 Sucrose fatty acid _ _ 〇〇 〇〇 〇〇 〇〇 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- 4- Ingot 10 In addition to the solid extract of 萃取.5g after the dry extract of 1-2 obtained by using 2H1 in Example 1-1, the soybean-containing hypocotyl fermented product was replaced with the above-mentioned Example 4- A mint ingot was produced under the same conditions as E-13. Example 4-E-15 Eucalyptus Ingots The following ingredients were used as raw materials, and the yoghurt bonds were produced according to the general production method of the tablets. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 4.00 (g) Trehalose (granulation) 88.80 Sucralose 〇.〇8 Powder fermented milk 5.00 20 Anhydrous citric acid 1.00 Yogurt 0.20 Sucrose fatty acid vinegar _1.50 Example 4-E-16 Ingots In addition to the solid extract of 萃取.5g after the extract of 1-2 dried 涸 114 200906315 obtained in Example 1-1, 75. An yoghurt bond was produced under the same conditions as in the above Example 4-E-15 except for the shaft ferment. Example 4-E-17 Lemon ingot The following ingredients were used as raw materials, and a rod-like key was produced in accordance with the general production method of the tablet. Soybean hypocotyl ferment containing equol (Reference 1-1) 4.00 (g) Sugar (granulation) 89.50 Powdered lemon juice 2.00 L-ascorbic acid 3.00 10 Anhydrous citric acid 1.00 Sucrose fatty acid ester _1.00 Example 4- E-18 The lemon ingot is prepared in addition to the solid extract of 萃取5 g of the extract 1-2 obtained by using 75Et in Example 1-1, instead of the soybean hypocotyl fermentate 15 containing the espresso. The screw-bonding key was produced under the same conditions as in Example 4-E-17. Example 4-E-19 Mint Chewing Gum The mint chewing gum was produced in accordance with the general production method of chewing gum using the following ingredients as a raw material. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-1) 4.00 (g) 20 Chewing gum base 25.00 Lactitol 83.20 Sugar alcohol 5.00 Sucralose 0.13 Glycerin 0.50 115 200906315 Peppermint oil 1.50 Mint spice 0.50 Colorant_0.20 Implementation Example 4-E-20 Mint Chewing Gum 5 In addition to the solid extract of 萃取5 g of the extract 1-2 obtained by using 75Et in Example 1-1, instead of the equol-containing soybean hypocotyl fermentate, Peppermint gum was produced under the same conditions as in the above Example 4-E-19. Example 4-E-21 Apple Bubble Gum Apple bubble gum was prepared according to the general production method of bubble gum using the following ingredients as a raw material. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-1) 4.00(g) Bubble gum matrix 30.00 Isatin (palatinit) 70.00 Sucralose 0.084 15 Glycerin 0.50 Citric acid (crystalline) 1.00 Confectionery _0-80 Example 4-E-22 Apple bubble gum In addition to 0.5 g of the solid extract obtained by using 75Et of the extract 1-2 dried mash 20 obtained in Example 1-1, the equol-containing soybean hypocotyl fermentate was replaced. Apple bubble gum was produced under the same conditions as in the above Example 4-E-21. Example 4-E-23 Strawberry chewing gum Strawberry chewing gum was produced in accordance with the general production method of chewing gum using the following ingredients as raw materials. 116 200906315 Soybean hypocotyl ferment containing equol (Reference 1-3) 4.00(g) Chewing gum base 23.00 Sugar powder 82.00 Maltose 5.00 5 Sucralose 0.001 Citric acid (crystal) 1.20 Purple lettuce pigment 0.20 Essence of raspberry _0.20 Example 4-E-24 Strawberry Chewing Gum 10 In addition to the solid extract of 5% of the extract 1-2 obtained by using 75Et in Example 1-1, instead of the equol-containing soybean hypocotyl fermentate Strawberry chewing gum was produced under the same conditions as in the above Example 4-E-23. Example 4-E-25 Chocolate The following ingredients were used as raw materials, and chocolate was obtained according to the general method of producing chocolate 15. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (g) Cocoa mass 40.00 Cocoa butter 25.00 Reduced powdered sugar 50.00 20 Sucralose 0.03 Lecithin 0.40 Vanilla extract _0.05 Example 4-E-26 Chocolate In addition to the solid extract of 萃取.5g after extracting 7.5 063 117 200906315 obtained in 75 1-1 in Example 1-1, the equol-containing soybean hypocotyl fermentate was replaced with the above Example 4- E-25 produces chocolate under the same conditions. Example 4-E-27 Strawberry-flavored chocolate Strawberry-flavored chocolate was prepared according to the general method for producing chocolate according to the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (g) Cocoa butter 25.00 Hard butter 7.00 Whole milk powder 27.00 10 Sugar 41.00 Strawberry flavor 0.20 Beet red 0.50 Sucralose 0.001 Egg fat 0.30 15 Vanilla extract 0.02 Example 4-E-28 Strawberry-flavored chocolate In addition to the solid extract of 萃取.5g after the use of 75Et of extract 1-2 obtained in Example 1-1, the soybean-containing hypocotyl fermented product was replaced. In addition, a strawberry flavor of 20 g of force was produced under the same conditions as in the above Example 4-E-27. Example 4-E-29 Cookies The following ingredients were mixed to make a raw meal which was baked in an oven to prepare a cookie. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) 118 200906315 Low-gluten flour 48.50 Hair powder 0.30 Salt-free milk Marlene 24.00 Salt 0.20 Sugar 12.00 Egg yolk 4.70 Trehalose 22.00 Spice 0.20 Water 40.00 10 Example 4-E-30 Cookies In addition to 0.05 parts by weight of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1, the equol-containing soybean hypocotyl fermentate was replaced. The cookies were produced under the same conditions as in the above Example 4-E-29 except for the above. 15 Example 4-E-31 Bun rind The bunion skin was obtained according to the general production method of the bun skin using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Ref. 1-2) 4.00 (parts by weight) Low-gluten flour 500.00 20 Hair powder 2.50 Sanxianjiao 1.00 Salt 8.00 Sugar 30.00 Trehalose 30.00 119 200906315 Dry yeast lard water 7.50 15.00 200.00 Example 4-E-32 Bun rind 5 In addition to 0.5 parts by weight of the solid extract obtained by using 75 Et of the extract 1-2 obtained in Example 1-1, instead of the equol-containing soybean hypocotyl fermentate The bun was prepared under the same conditions as in the above Example 4-E-31. Example 4-E-33 Multi-Person Fruit 10 Use the following ingredients to make apple pie. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-1) 0.40 (parts by weight) Apple 200.00 Maltose 150.00 Sucralose 0.10 15 Bar-shaped juice 10.00 Walnut 80.00 Raisin 80.00 Apple flavor 0.20 Example 4-E-34 Apple The filling stuffing 20 was replaced with the equol-containing soybean hypocotyl fermentate in addition to 0.05 parts by weight of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1, in combination with the foregoing Example 4 -E-33 is the same condition to make apple pie. Example 4-E-35 Snow Cake 120 200906315 The following ingredients were mixed to prepare a raw material, which was baked in an oven to obtain a snow cake. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-1) 0.40 (parts by weight) 100.00 100.00 0.03 0.03 100.00 0.22 120.00 1.50 Cream 5 Reduction Maltose Sucrose Sucrose Leaf Whole Egg Flavor 10 Low-gluten flour expansion agent _ Example 4-E-36 Snow cake, in addition to 0.05 parts by weight of the solid extract obtained by using 75E Et of the extract 1-2 in the example Μ, in place of the equol-containing soybean hypocotyl 15 fermentate, A snow cake was produced under the same conditions as in Example 4-E-35. Example 4-E-37 Fern Cake The fern cake was produced using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-1) 0.40 (parts by weight) 20 m starch 20.00 Sweet potato starch 40.00 Sugar 8.00 ZK_Residual portion 100.00 parts by weight total 121 200906315 Example 4-E-38 Fern cake In addition to the equol-containing soybean hypocotyl fermentate in place of 5 parts by weight of the solid extract 〇·〇 after the extraction of the extraction solution 1-2 obtained in 75Et in Example 1-1, the same as in the foregoing Example 4 - Ε-37 produces the fern cake under the same conditions. f Example 4-Ε-39 Bean Fruit Use the following ingredients to make bean fruit. Soybean hypocotyl fermented product containing equol (Reference Example 1-3) 8.00 (parts by weight) Hanmei powder 7.00 Sugar 53.00 10 Huang Dan powder 34.00 Expansion agent Appropriate amount of flavor and amount of sweeteners Appropriate amount 15 Example 4-E-40 Bean 100.00 parts by weight of the fruit, in addition to 0.75 parts by weight of the solid extract after the extraction of the extraction liquid 1-2 obtained in 75Et in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, with the foregoing examples Bean fruit was produced under the same conditions as 4-E-39. 20 Example 4-E-41 Bean fruit Bean fruit was produced using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 12.00 (parts by weight) glutinous rice flour 13.00 Sugar 45.00 122 200906315 Soy flour 15.00 Matcha 15.00 Expanding agent appropriate amount of flavoring amount of sweeteners Appropriate amount 100.00 parts by weight Example 4- The E-42 bean fruit was replaced with the equol-containing soybean hypocot 10 fermentation product in addition to 1.5 parts by weight of the solid extract obtained by using 75E of the extract 1-2 obtained in the Example Μ, in addition to the foregoing examples. Bean fruit was produced under the same conditions as 4-E-41. Example 4-E-43 Bean Fruit Bean fruit was produced using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-1) 18.00 (parts by weight) 15 Hanmei powder 15.00 Sugar 52.00 Soy flour 15.00 Matcha 12.00 Expansion agent amount 20 Spices appropriate amount of sweeteners Appropriate amount 100.00 parts by weight Example 4- E-44 Beanseed was replaced with the equol-containing soybean hypocotyl fermentate in addition to 2.25 parts by weight of the solid extract obtained by using 75E Et of the extraction solution 1-2 dried 涸123 200906315 in the example Μ Example 4-E-43 Bean Fruit 0 was produced under the same conditions as in Example 4-E-45 Bean Fruit 5 Bean fruit was produced using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 8.00 (parts by weight) Starch 20.00 Sugar 32.00 Yellow ugly powder 10.00 10 Black sesame 20.00 Expanding agent appropriate amount of flavoring amount of sweetener appropriate amount 100.00 parts by weight 15 Example 4 -E-46 Bean fruit, in addition to 0.75 parts by weight of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, Example 4-E-45 was made under the same conditions to make bean fruit. 20 Example 4-E-47 Raw bran fruit Raw bran fruit was produced using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 10.00 (parts by weight) gluten glutinous rice flour 20.00 15.00 124 200906315 Soy flour powder emulsifier appropriate amount sorbitol 20.00 5 chocolate 30.00 Example 4 -E-48, in addition to the 1.25 parts by weight of the solid extract obtained by using the extraction solution 1-2 obtained in 75Et in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, Raw bran 10 fruit was produced under the same conditions as in the above Example 4-E-47. Example 4-E-49 Muffin (Mixed) A muffin (flavored type) was prepared according to the general method for producing a muffin using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) 15 Flour 100.00 Sugar 2.00 Grease 3.00 Whole egg 3.00 Skim milk powder 2.00 20 Acid canine 0.30 Milk 50.00 Water 100.00 Example 4-E -50 muffin (sweet type) In addition to 0.05 parts by weight of the solid extract after extracting 1-2 dry 涸 125 200906315 obtained in Example 1-1 using 75Et, the equol-containing soybean hypocotyl fermentate was replaced. A muffin (rich flavor type) was produced under the same conditions as in the above Example 4-E-49. Example 4-E-51 Muffin (Original) 5 Muffins (original flavor) were prepared according to the general manufacturing method of muffins using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) Flour 100.00 Oil 2.50 0.30 0.40 0.30 0.05 10 Whole egg ammonium carbonate sodium carbonate fat-filled water 150.00 15 Example 4-E- 52 muffin (original flavor), in addition to the 0.05% by weight of the solid extract after the extraction of the extraction solution 1-2 obtained in 75 Et in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, The muffin (original flavor) was produced under the same conditions as in Example 4-E-51. 20 Example 4-E-53 Cheese biscuits The cheese biscuits were prepared according to the general production method using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (w) by weight Flour 100.00 Oil 9.00 126 200906315 Malt extract 1.30 Carbon sodium hydrogenate 0.80 Spice 0.10 Cheese powder 13.00 Sugar 80.00 Salt 0.90 Ammonium carbonate 0.80 Lecithin 0.05 Water 33.00 10 Example 4-E-54 The cheese biscuit was replaced by 0.05 part by weight of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1. In addition to the soybean hypocotyl fermentate, a cheese biscuit was produced under the same conditions as in the above Example 4-E-53. 15 Example 4-E-55 Alpaca The alpaca was prepared according to the general manufacturing method using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (parts by weight) 7.50 320.00 820.00 50.00 300.00 Amaranth sugar 20 Red bean maltose ^_ Example 4-E-56 In addition to the compound 1-1 In the same manner as in the above-mentioned Example 4-E-55, except that 0.5 parts by weight of the extract 1-2 obtained after 75Et was used, 0.5 parts by weight of the solid extract after 200906315 was substituted for the equol-containing soybean hypocotyl fermentate. Alpaca. Example 4-E-57 Soft Cookies Using the following ingredients, soft cookies were prepared in accordance with a general manufacturing method. 5 Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (parts by weight) Flour 200.00 Cream 150.00 Sugar 80.00 Whole egg 150.00 10 Example 4-E-58 Soft cookies In addition to Example 1 In the same manner as in the above Example 4-E-57, except that 0.5 parts by weight of the solid extract of the extract 1-2 obtained by 75 Et was used in place of the equol-containing soybean hypocotyl fermentate. Cookies. 15 Example 4-E-59 Scallop Scallop was prepared according to a general production method using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) stalked rice 100.00 hot water _ appropriate amount 20 Example 4-E-60 Scallop is obtained by using 75Et in combination with Example 1-1 The scallops were prepared under the same conditions as in the above Example 4-E-59 except that 0.5 parts by weight of the solid extract of the extract 1-2 was dried instead of the equol-containing soybean hypocotyl fermentate. Example 4-E-61 Chabeng Cake 128 200906315 The Chuanbin cake was prepared according to the general production method using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (parts by weight) 800.00 400.00 130.00 180.00 White sugar soy flour maltose ^_ Example 4-E-62 In addition to the compound 1-1 The berry cake was produced under the same conditions as the above-mentioned Example 4-E-61 except that 0.5 parts by weight of the solid extract of the extract 1-2 obtained by 75 Et was used instead of the equol-containing soybean hypocot 10 fermentate. . Example 4-E-63 Thousand Fruits Thousands of fruits were produced according to the general production method using the following ingredients. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (parts by weight) 500.00 25.00 75.00 15 and three pots of sugar Shitori Hanmei powder Example 4-E-64 Thousand fruits used in addition to Example 1-1 Thousands of fruits were produced under the same conditions as in the above Example 4-E-63, except that 0.5 parts by weight of the solid extract of the extract 1-2 obtained after 75 Et was used instead of the equol-containing soybean hypocotyl fermentate. Example 4-E-65 Fruit Soft Sugar The fruit soft candy was produced according to a general production method using the following ingredients. 129 200906315 Soybean hypocotyl fermented product containing equol (Reference example sugar maltose gelatin 4·〇〇 (parts by weight) 30.00 5〇.〇〇7.〇〇 Fruit juice citric acid spice 5.00 1.5〇 Example 4-Ε- 66 fruit soft candy

除了配合實施例Μ中使用7%獲得之萃取則却 固態狀萃取物〇.5重量份來取代含雌馬酚之大豆：固 物以外，以與前述實施例4_E_65相同之條件製造軸 軟膠糖。 實施例4-E-67 含大豆粉之供烤點 除了 1_25重量％含雌馬酚之大豆胚軸發酵物（參考如A shaft soft gum was produced in the same manner as in the above Example 4_E_65 except that the extract obtained by using 7% was used in the Example 却, but the solid extract was 〇. 5 parts by weight in place of the equol-containing soybean: solid. . Example 4-E-67 Soybean meal-containing baking point except 1_25% by weight of equol-containing soybean hypocotyl fermentate (refer to

粒、可可粉、食鹽、膨脹劑及香料，使所得混合原料（1〇〇 重量％)以成型機成形為長方形（約丨5cmxl 5cmxl〇cm)，以 烤箱烘焙18(TC而製得含大豆粉之供烤點心。 20實施例4-E-6R_含大豆粉之烘烤點心 除了使用係配合有實施例1-1中使用75Et獲得之萃取 液1-2乾涸後的固態狀萃取物〇.25重量份來取代含雌馬酚之 大豆胚軸發酵物的混合原料以外，以與前述實施例4-E-67 相同之條件製造含大豆粉之烘烤點心。 130 200906315 實施例4-F 調味料 以下，就本發明之調味料製造例列舉具體例。 實施例4-F-1 烤肉醬 5 製造下述組成之烤肉醬。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(Kg) 還原麥芽糖 20.00 海藻糖 0.50 味琳 4.00 濃味醬油 24.00 10 蘋果泥 19.00 大蒜泥 4.50 生薑泥 4.50 芝麻油 0.10 三仙膠 0.50 15 紅椒末 0.10 黑胡椒末 0.05 檸檬酸(結晶） 0.20 烈芝麻 0.40 水 殘餘部分 20 合計 lOO.OOKg 實施例4-F-2 烤肉醬 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-F-1相同之條件製造烤肉醬。 131 200906315 實施例4-F-3 番茄醬 製造下述組成之番茄醬。 含雌馬紛之大豆胚軸發酵物（參考例1-2) 4.〇〇(Kg) 番祐嘗 40.00 5 葡萄酒醋 18.00 果糖葡萄糖液糖 5.00 濃味醬油 24.00 食鹽 2.50 大蒜粉 0.07 10 洋蔥粉 0.10 肉桂粉 0.02 丁香粉 0.01 水 殘餘部分 合計 lOO.OOKg 15 實施例4-F-4 番茄醬 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-F-3相同之條件製造番祐醬。 實施例4-F-5 醬油 20 製造下述組成之醬油（天婦羅醬油）。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 4.00(Kg) 砂糖 7.00 濃味醬油 28.00 淡味醬油 10.00 132 200906315 魚醬 5.00 味琳 10.00 L-麵胺酸鈉 3.00 食鹽 0.80 水 殘餘部分 合計 lOO.OOKg 實施例4-F-6 醬油 除了配合實施例Μ中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5Kg來取代含雌馬盼之大豆胚軸發酵 10 物以外，以與前述實施例4-F-5相同之條件製造醬油。 實施例4-F-7 沾醬 以7 : 3重量比混合下述組成之水相與油相，而製得沾 醬（分離型）。 水相組成 15 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(Kg) 砂糖 8.00 濃味醬油 20.00 釀造醋（酸度10%) 8.50 魚醬 3.00 20 檸檬果汁 2.00 食鹽 2.00 L-麩胺酸鈉 0.20 紅椒末 0.05 煎芝麻 0.30 133 200906315 三仙膠 0.10 水 殘餘部分 合計 lOO.OOKg 油相組成 5 芝麻油 20.00(Kg) 玉米沙拉油 79.00 調咮香料 1.00 合計 lOO.OOKg 實施例4-F-8 沾醬 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-F-7相同之條件製造沾醬（分離 型）。 實施例4-F-9 涮涮鍋之芝麻醬 15 製造下述組成之涮涮鍋芝麻醬。 含雌馬酚之大豆胚軸發酵物（參考例1-2) 0.40(Kg) 淡味醬油 12.00 釀造醋 11.00 大蒜粉 0.10 20 薑粉 0.10 白胡椒 0.10 雞肉精 3.00 酵母萃取 3.00 芝麻油 1.00 134 200906315 果糖葡萄糖液糖 8.00 味琳 15.00 白味噌 10.00 增黏劑 3.50 砂糖 8.00 食鹽 10.00 香料 0.15 水 殘餘部分 合計 lOO.OOKg 10 貫施例4-F-10 >刷測銷芝麻醬 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-F-9相同之條件製造涮涮鍋芝麻 醬。 15 實施例4-F-11 烤肉醬（味噌口味） 製造下述組成之烤肉醬（味噌口味）。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 4.00(Kg) 濃味醬油 25.00 味噌 14.00 20 D-山梨糖醇 12.00 食鹽 3.20 大蒜粉 3.30 大蒜泥 2.00 牛肉精 1.00 135 200906315 烈芝麻 0.50 L-糙胺酸鈉 0.50 生薑泥 0.40 胺基酸調味料 0.01 增黏劑 3.50 砂糖 10.00 水 殘餘部分 合計 lOO.OOKg 實施例4-F-12 烤肉醬（味噌口味） 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-F-11相同之條件製造烤肉醬（味 口曾口味）。 實施例4-F-13 零嘴調味料（snack seasoning) 15 製造下述組成之零嘴調味料。 含雌馬酚之大豆胚轴發酵物(參考例1-3) 0.40(重量份) 食鹽 14.00 磷酸三鈣 3.00 琥珀酸二鈉 0.20 20 L-麩胺酸鈉 7.00 粉末醬油 3.00 酵母萃取物 1.50 洋蔥粉 1.50 甜椒粉 1.50 136 200906315 大蒜粉 8.00 辣椒粉末 0.50 清肉湯(consomm0) 21.00 牛肉汁 4.00 甜菊葉 0.80 葡萄糖 13.00 香辛料 0.01 玉米澱粉 23.00 砂糖 2.00 10 實施例4-F-14 零嘴調味料 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-F-13相同之條件製造零嘴調 味料。 15 實施例4-F-15 乳化醬油 製造下述組成之乳化醬油。 含雌馬酚之大豆胚軸發酵物(參考例1-2) 0.40(重量份) 沙拉油 34.00 濃味醬油 53.00 20 阿拉伯膠 1.00 砂糖_12.00 實施例4-F-16 乳化醬油 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 137 200906315 發酵物以外，以與前述實施例4-F-15相同之條件製造乳化醬 油。 實施例4-F-17 沾醬 製造下述組成之沾醬（乳化型）。 5 含雌馬酌·之大豆胚軸發酵物(參考例1-2) 0.40(重量份) 沙拉油 35.00 食醋 10.00 砂糖 11.00 食鹽 4.00 10 三仙膠 0.30 水 殘餘部分 合計 100.00重量份 實施例4-F-18 沾醬 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 15 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-F-17相同之條件製造沾醬 (乳化型）。 實施例4-F-19 蒲燒醬 製造下述組成之蒲燒醬。 20 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份) D-山梨糖醇 10.00 增黏劑 0.30 濃味醬油 38.00 味琳 20.00 138 200906315 10 甜味精 2.00 食鹽 1.80 L-麵胺酸鈉 1.45 肌苷酸鈉 0.025 鳥苷酸鈉 0.025 海藻糖 15.00 砂糖 8.00 焦糖色素 0.50 洋蔥色素 0.10 香料 0.50 水 殘餘部分 合計 100.00重量份 實施例4-F-20 蒲燒醬 15 20 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-F-19相同之條件製造蒲燒醬 實施例4-F-21 花枝珍味之調味料 製造下述組成之花枝珍味調味料。 含雌馬酚之大豆胚軸發酵物(參考例1-2) 0.40(重量份) 濃味醬油 20.00 淡味醬油 10.00 5.00 魚醬 咮琳 10.00 釀造醋 2.00 139 200906315 砂糖 38.00 食鹽 1.00 L-麩胺酸鈉 2.00 甜味精 1.40 琥珀酸二鈉 0.20 大蒜粉 0.05 水 殘餘4 合計 100.00重量份 實施例4-F-22 花枝珍味之調味料 10 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-F-21相同之條件製造花枝珍 味之調味料。 實施例4-G 殺菌袋食品 15 以下，就本發明之殺菌袋食品製造例列舉具體例。 實施例4-G-1 殺菌袋咖哩 依定法製造下述組成之殺菌袋咖哩。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 1.80 咖哩粉 20 洋蔥 7.00 無鹽奶油 4.50 大蒜泥 0.80 生薑泥 0.50 麵粉 4.50 140 200906315 調味果醬（fruit chutney) 3.00 番茄膏 0.50 食鹽 0.55 L-麩胺酸鈉 0.48 5 核酸調味料 0.015Granules, cocoa powder, salt, swelling agent and perfume, the obtained mixed raw material (1% by weight) is formed into a rectangular shape by a molding machine (about cm5cmxl 5cmxl〇cm), and baked in the oven 18 (TC) to obtain soy flour For the purpose of baking the snacks. 20 Example 4-E-6R_ Soybean flour-containing baking dessert In addition to using the solid extract obtained by mixing the extract 1-2 obtained in Example 1-1 with 75Et. A soy flour-containing baked snack was produced under the same conditions as in the above Example 4-E-67, except that 25 parts by weight of the mixed raw material of the equol-containing soybean hypocotyl fermentate was replaced. 130 200906315 Example 4-F Seasoning In the following, a specific example is given for the preparation of the seasoning of the present invention. Example 4-F-1 Barbecue Sauce 5 The barbecue sauce of the following composition was produced. The equol-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00(Kg) Reduction Maltose 20.00 Trehalose 0.50 Wei Lin 4.00 Concentrated Soy Sauce 24.00 10 Apple Mud 19.00 Garlic Mud 4.50 Ginger Mud 4.50 Sesame Oil 0.10 Sanxianjiao 0.50 15 Red Pepper 0.10 Black Peppermint 0.05 Citric Acid (Crystalline) 0.20 Sesame 0.40 water residue Part 20 Total lOO.OOKg Example 4-F-2 Barbecue Sauce In addition to the solid extract of 萃取5Kg used in the extraction of 1-2 5% of the extract 7.5 obtained in Example 1-1, the equol-containing soybean was replaced. In addition to the hypocotyl fermentate, barbecue sauce was prepared under the same conditions as in the above Example 4-F-1. 131 200906315 Example 4-F-3 Ketchup The following composition of ketchup was produced. Fermented product (Reference Example 1-2) 4. 〇〇 (Kg) 番佑味40.00 5 wine vinegar 18.00 fructose glucose liquid sugar 5.00 concentrated soy sauce 24.00 salt 2.50 garlic powder 0.07 10 onion powder 0.10 cinnamon powder 0.02 clove powder 0.01 water residue Partially total lOO.OOKg 15 Example 4-F-4 Ketchup In addition to the solid extract in the same manner as in Example 1-1 using 75Et of extract 1-2, the dried solid extract 〇5Kg was substituted for the equol-containing soybean. In addition to the hypocotyl fermentate, the savory sauce was produced under the same conditions as in the above Example 4-F-3. Example 4-F-5 Soy Sauce 20 The following composition of soy sauce (tempura soy sauce) was produced. Soybean hypocotyl ferment (Reference Example 1-2) 4.00 (Kg) Sugar 7.00 Soy Sauce 28.00 Light Soy Sauce 10.00 132 200906315 Fish Sauce 5.00 Wei Lin 10.00 L-Sodium Amidinate 3.00 Salt 0.80 Water Residue Total lOO.OOKg Example 4-F-6 Soy Sauce is used in addition to 75Et in the Example Soy sauce was produced under the same conditions as in the above Example 4-F-5, except that the extract of the extract 1-2 was dried and the solid extract of 。. 5 Kg was substituted for the soybean hypocotyl fermentation of the equator. Example 4 - F-7 Dip Sauce The water phase and the oil phase of the following composition were mixed at a weight ratio of 7:3 to prepare a sauce (separated type). The water phase consists of 15 equol-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (Kg) Sugar 8.00 Soy sauce 20.00 Brewed vinegar (acidity 10%) 8.50 Fish sauce 3.00 20 Lemon juice 2.00 Salt 2.00 L- Sodium glutamate 0.20 Red pepper end 0.05 Fried sesame 0.30 133 200906315 Sanxianjiao 0.10 Water residue total lOO.OOKg Oil phase composition 5 Sesame oil 20.00 (Kg) Corn salad oil 79.00 Tart spice 1.00 Total lOO.OOKg Example 4- F-8 Sauce 10 In addition to the solid extract of 萃取5Kg after the extraction of 1-2 5% of the extract 1-2 obtained in Example 1-1, the equol-containing soybean hypocotyl fermentate was replaced with Example 4 - F-7 The same conditions were used to make a dip (separate type). Example 4-F-9 Tahini Sauce 15 A shabu-shabu sesame sauce having the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 0.40 (Kg) Light soy sauce 12.00 Brewed vinegar 11.00 Garlic powder 0.10 20 Ginger powder 0.10 White pepper 0.10 Chicken essence 3.00 Yeast extract 3.00 Sesame oil 1.00 134 200906315 Fructose glucose solution Sugar 8.00 Wei Lin 15.00 White miso 10.00 Adhesive 3.50 Sugar 8.00 Salt 10.00 Perfume 0.15 Water residue total lOO.OOKg 10 Example 4-F-10 > Brush test pin sesame paste in addition to the use of 75Et in Example 1-1 A crucible sesame sauce was prepared under the same conditions as in the above Example 4-F-9 except that the obtained extract 1-2 was dried and the solid extract 〇 5 Kg was used instead of the equol-containing soybean hypocotyl fermentate. . 15 Example 4-F-11 Barbecue Sauce (Miso Taste) The following composition of barbecue sauce (Miso taste) was produced. Soybean hypocotyl ferment containing equol (Reference 1-3) 4.00 (Kg) Soy sauce 25.00 Miso 14.00 20 D-sorbitol 12.00 Salt 3.20 Garlic powder 3.30 Garlic puree 2.00 Beef extract 1.00 135 200906315 Sesame sesame 0.50 L-rough acid sodium 0.50 Ginger mud 0.40 Amino acid seasoning 0.01 Adhesive agent 3.50 Sugar 10.00 Water residue total lOO.OOKg Example 4-F-12 Barbecue sauce (Miso taste) 10 In addition to the example 1 In the first step, the same conditions as in the above-mentioned Example 4-F-11 were carried out except that the solid extract of 7.5 g of the extract 1-2 obtained by the extraction of 7.5 Torr was used in place of the equol-containing soybean hypocotyl fermentate. Barbecue sauce (taste tastes). Example 4-F-13 Snack seasoning 15 A snack seasoning of the following composition was produced. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) salt 14.00 Tricalcium phosphate 3.00 Sodium succinate 0.20 20 L-sodium glutamate 7.00 Powder soy sauce 3.00 Yeast extract 1.50 Onion powder 1.50 Sweet pepper powder 1.50 136 200906315 Garlic powder 8.00 Chili powder 0.50 Clear broth (consomm0) 21.00 Beef juice 4.00 Stevia leaves 0.80 Glucose 13.00 Spice 0.01 Corn starch 23.00 Sugar 2.00 10 Example 4-F-14 Zero mouth seasoning in addition to the examples In the same manner as in the above-mentioned Example 4-F-13, except that 0.05 parts by weight of the solid extract of the extract 1-2 obtained by 75Et was used in place of the equol-containing soybean hypocotyl fermentate in 1-1. Make a small mouth seasoning. 15 Example 4-F-15 Emulsified Soy Sauce An emulsified soy sauce of the following composition was produced. Soybean hypocotyl fermented product containing equol (Reference Example 1-2) 0.40 (parts by weight) Salad oil 34.00 concentrated soy sauce 53.00 20 gum arabic 1.00 granulated sugar _12.00 Example 4-F-16 emulsified soy sauce In addition to the example 1 -1 in the same manner as in the above Example 4-F-15 except that 0.05% by weight of the solid extract of the extract 1-2 obtained by 75Et was used instead of the equol-containing soybean hypocotyl 137 200906315 fermentate. Conditions to make emulsified soy sauce. Example 4-F-17 Dip Sauce The following composition of the sauce (emulsified type) was produced. 5 Soybean hypocotyl fermented product containing female horses (Reference 1-2) 0.40 (parts by weight) Salad oil 35.00 Vinegar 10.00 Sugar 10.00 Salt 4.00 10 Sanxian gum 0.30 Water residue total 100.00 parts by weight Example 4- F-18 is mixed with 0.05% by weight of the solid extract obtained by using 75Et of the extract 1-2 dried extract 15 obtained in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, Example 4 - F-17 was used to produce a sauce (emulsion type). Example 4-F-19 Poached Sauce The following composition was prepared. 20 Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (w) by weight D-sorbitol 10.00 Adhesive 0.30 Rich soy sauce 38.00 Wei Lin 20.00 138 200906315 10 Sweet MSG 2.00 Salt 1.80 L- Sodium facetete 1.45 Sodium inosine 0.025 Sodium guanosine 0.025 Trehalose 15.00 Sugar 8.00 Caramel color 0.50 Onion pigment 0.10 Perfume 0.50 Water residue total 100.00 parts by weight Example 4-F-20 Poached sauce 15 20 In addition to cooperation In Example 1-1, the extract of the extract 1-2 obtained by 75 Et was used in an amount of 0.05 part by weight of the dried solid extract to replace the equol-containing soybean hypocotyl fermentate, in the same manner as in the above Example 4-F-19. Conditions for the manufacture of the simmered sauce Example 4-F-21 The scent of the scented scented scented scented scented seasoning. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-2) 0.40 (w) by weight soy sauce 20.00 light soy sauce 10.00 5.00 Fish sauce Yulin 10.00 Brewing vinegar 2.00 139 200906315 Sugar 38.00 Salt 1.00 L-glutamate 2.00 Sweet MSG 1.40 Sodium succinate 0.20 Garlic powder 0.05 Water residue 4 Total 100.00 parts by weight Example 4-F-22 Flowering seasoning seasoning 10 In addition to the extract 1-2 obtained in Example 1-1 using 75Et A flavor of the scented scent was prepared in the same manner as in the above Example 4-F-21, except that 0.05 parts by weight of the solid extract was used instead of the equol-containing soybean hypocotyl fermentate. Example 4-G Sterilization Bag Foods 15 Hereinafter, specific examples of the production of the sterilization bag foods of the present invention will be described. Example 4-G-1 Sterilization Bag Curry A sterilized bag curry of the following composition was produced according to the method. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) 1.80 Curry powder 20 Onion 7.00 Unsalted cream 4.50 Garlic puree 0.80 Ginger puree 0.50 Flour 4.50 140 200906315 Flavored jam (fruit chutney) 3.00 Tomato Cream 0.50 salt 0.55 L-sodium glutamate 0.48 5 nucleic acid seasoning 0.015

Demiglace風味調味料 1.00 豬肉精調味料 0.50 肉羹(bouillon)基底調味料 1.00 馬鈴薯 7.00 10 紅蘿蔔 5.00 牛肉 10.00 ZK：_殘餘部分 合計 lOO.OOKg 實施例4-G-2 殺菌袋咖哩 15 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-G-1相同之條件製造殺菌袋咖 口审〇 實施例4-G-3 殺菌袋中華丼素 20 依定法製造下述組成之殺菌袋中華丼素。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 淡味醬油 72.00 酒 48.00 食鹽 4.00 141 200906315 5 10 L-麩胺酸鈉 1.30 核酸調味料 0.07 雞肉精粉 1.00 砂糖 7.00 水 209.00 豬肉 200.00 花枝 120.00 蝦 80.00 香蕈(shiitake) 70.00 水煮竹筍 180.00 紅蘿蔔 80.00 洋篦 240.00 實施例4-G-4 殺菌袋中華丼素 15 20 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-G-3相同之條件製造殺菌袋中華 丼素。 實施例4-H 食用加工肉品 茲就本發明之食用加工肉品製造例列舉具體例如下。 實施例4-H-1 香腸 使用下述組成原料，依照香腸之一般製造方法而製得 香腸。 肉部 豬腳肉5mm絞碎 70.00(Kg) 142 200906315 豬油5mm絞碎 冰水 10.00 20.00 合計 lOO.OOKg 副原料 5 含雌馬酚之大豆胚軸發酵物（參考例1-3) 〇.40(Kg) 食鹽 1.70 亞梢酸鈉 0.012 聚合磷酸鹽 0.30 L-抗壞血酸鈉 1.00 10 酪蛋白鈉 0.50 白胡椒末 0.10 山梨酸鉀 0.20 胺基酸調味料 0.30 綜合香辛料 0.50 15 實施例4-H-2 香腸 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 物以外，以與前述實施例4-H-1相同之條件製造香腸。 實施例4-H-3 煙燻香腸 20 以18 : 1之比例混合下述組成之肉部與調味料，依照煙 燻香腸之一般製造方法來製造煙燻香腸。 肉部 豬腳肉餡 85.00(Kg) 全蛋 4.00 143 200906315 牛奶 3.00 冰水 調味料 7.00 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 5 食鹽 24.00 胺基酸調味料 3.20 香辛料 8.85 碎洋蔥 18.00 砂糖 10 實施例4-H-4 煙燻香腸 48.00 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-H-3相同之條件製造煙燻香腸。 實施例4-H-5 漢堡肉 15 依定法製造下述組成之漢堡肉。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0_40(重量份） 牛豬混合絞肉 45.00 豚肉 9.00 洋蔥 12.00 20 全蛋 5.00 粒狀大豆蛋白 9.00 酷蛋白鈉 5.00 鹿角菜膠 1.50 刺槐豆膠 0.50 144 200906315 香辛料 0.30 酵母萃取 0.20 食鹽 1.00 砂糖 1.20 5 水溶性食物纖維 1.00 ZK：_殘餘部分 合計 100.00重量份 實施例4-Η-6 漢堡肉 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 10 後的固態狀萃取物0.05重量份來取代含雌馬紛之大豆胚軸 發酵物以外，以與前述實施例4-H-5相同之條件製造漢堡 肉。 實施例4-1揉合食品 以下列舉本發明之揉合食品製造例的具體例。 15 實施例4-1-1 炸魚板 使用下述組成原料，依照炸魚板之一般的製造方法而 製得炸魚板。 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 魚肉 50.00 20 食鹽 1.50 胺基酸調味料 0.30 甘胺酸 0.50 無水乙酸鈉 0.30 馬鈴薯澱粉 8.00 145 200906315 味琳 1.00 冰水_殘餘部分 合計 lOO.OOKg 實施例4-1-2 炸魚板 5 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚轴發酵 物以外，以與前述實施例4-1-1相同之條件製造炸魚板。 實施例4-J 蛋加工食品 以下就本發明之蛋加工食品之製造例列舉具體例。 10 實施例4-J-1 厚煎蛋 使用下述組成之原料，依照厚煎蛋之一般製造方法製 造厚煎蛋。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份） 全蛋 70.00 15 食鹽 0.40 澱粉 2.00 尚湯 2.00 砂糖 1.20 乙酸鈉 1.00 20 水 殘餘部分 合計 100.00重量份 實施例4-J-2 厚煎蛋 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚轴 146 200906315 發酵物以外，以與前述實施例4-J-1相同之條件製造厚煎蛋。 實施例4-K 罐頭或罐裝食品 以下，就本發明之罐頭或罐裝食品之製造例列舉具體 例。 5 實施例4-K-1 柑橘罐頭 將下述組成之糖漿與柑橘果肉封罐，以製得柑橘罐頭。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份) 砂糖 10.00 果糖葡萄糖液糖 10.00 10 檸檬酸 0.25 擰檬酸三鈉 0.10 ^_殘餘部分 合計 100.00重量份 實施例4-K-2 柑橘罐頭 15 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-K-1相同之條件製造柑橘罐 頭。 實施例4-L 麵包 20 以下就本發明麵包之製造例列舉具體例。 實施例4-L-1 吐司 使用下述組成之生料製造吐司。 中種 高筋麵粉 70.00(Kg) 147 200906315 酵母活化劑（yeast-food) 0.10 活酵母 2.00 水 40.00 本捏 5 含雌馬酚之大豆胚軸發酵物（參考例1-3) 0.40(Kg) 高筋麵粉 30.00 砂糖 3.00 食鹽 2.00 脫脂奶粉 2.00 10 穌油 5.00 水 25.00 實施例4-L-2 吐司 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物〇.〇5Kg來取代含雌馬酚之大豆胚軸發酵 15 物以外，以與前述實施例4-L-1相同之條件製造吐司。 實施例4-M 冷束點心 以下，就本發明冷凍點心之製造例列舉具體例。 膏施例4-M-1 冰淇淋 製造下述組成之冰淇淋。 20 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份) 鮮奶油 8.80 奶油 5.00 麥芽糖 18.00 脫脂奶粉 8.50 148 200906315 海藻糖 7.00 砂糖 5.00 安定化劑 0.20 香料 0.15 水 殘餘部分 合計 100.00重量份 實施例4-M-2 冰湛淋 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 10 發酵物以外，以與前述實施例4-M-1相同之條件製造冰淇 淋。 實施例4-N 大豆加工食品 以下，就本發明之大豆加工食品製造例列舉具體例。 實施例4-N-1 納豆 15 將蒸大豆99.8重量份與含雌馬酚之大豆胚軸發酵物（參 考例1-3)0·4重量份混合，接種納豆菌進行發酵，而製得納 豆。 實施例4-Ν-2 納豆 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 20 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-N-1相同之條件製造納豆。 實施例4-N-3 豆腐 製造下述組成之豆腐。 含雌馬酚之大豆胚轴發酵物(參考例1-3) 0.40(重量份） 149 200906315 99.30 0.20 0.10 豆乳 氯化鎂 山梨酸鉀 合計 100.00重量份。 5 實施例4-N-4 豆腐 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-N-3相同之條件製造立腐。 實施例4-0 米飯食品 10 以下，就本發明米飯食品之製造例列舉具體例。 實施例4-0-1 粥 使用下述組成原料，依照粥之一般製造方法而製得粥。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 0.40(重量份） 米 180.00 15 食鹽 1.00 ^_900.00 實施例4-0-2 粥 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.05重量份來取代含雌馬酚之大豆胚轴 20 發酵物以外，以與前述實施例4-0-1相同之條件製造粥。 實施例4-0-3 栗子糯米飯 使用下述組成之原料，依一般製造方法製得栗子糯米 飯。 含雌馬酚之大豆胚軸發酵物(參考例1-3) 4.00(重量份） 150 200906315 實施例4-0-4 栗子糯米飯 10 15 糯米 1000.00 剝殼栗子 300.00 面湯 230.00 黑芝麻 30.00 食鹽水 8.00 除了配合實施例1-1中使用75Et獲得之萃取液1-2乾涸 後的固態狀萃取物0.5重量份來取代含雌馬盼之大豆胚軸 發酵物以外，以與前述實施例4-0-3相同之條件製造栗子糯 米飯。 實施例4-P 湯品 以下，就本發明湯品之製造例列舉具體例。 實施例4-P-1 玉米奶油湯 使用下述組成之原料，依照湯品一般之製造方法，製 得玉米奶油湯。 含雌馬酚之大豆胚轴發酵物(參考例1-3) 0.40(重量份) 20 牛奶 180.00 玉米 190.00 清肉湯(consommi) 20.00 沙拉油 15.00 食鹽 適量 胡椒 適量 水 380.00 實施例4-P-2 玉米奶油湯 151 200906315 除了配合實施例1-1中使用75Et獲得之萃取液ι_2乾涸 後的固態狀萃取物0·05重量份來取代含雌馬酚之大豆胚軸 發酵物以外，以與前述實施例4-P-1相同之條件製造玉米奶 油湯。 5 【圖式簡單說明】 第1圖顯示檢測參考例M所得含雌馬酚之大豆胚軸發 酵物、大豆子葉及大豆胚轴所含總蛋白質之結果（電泳圖）。 第2圖顯示檢測參考例M所得含雌馬酚之大豆胚軸發 酵物、大豆子葉及大豆胚軸所含主要致敏原（Gym4、 10 Gm30K、Gm28K)之結果（電泳圖）。 第3圖顯示檢測參考例M所得含雌馬酚之大豆胚軸發 酵物、大豆子葉及大豆胚軸所含主要致敏原（7S球蛋白 mix、油體蛋白（Oleosins)、胰蛋白酶抑制因子（trypsin inhibitor))之結果（電泳圖）。 15 第4圖顯示實施例卜1中以TLC分析萃取液1_2及2-2之 雌馬酚含量的結果。 第5圖顯示實施例卜1中以TLC分析萃取液1-2及2-2之 皂苷含量的結果。 第6圖顯示實施例1-1中以TLC分析萃取液1-2及殘渣2 20 之雌馬酚含量的結果。 第7圖顯示實施例1-1中以TLC分析萃取液1-2及殘渣2 之皂苷含量的結果。 第8圖顯示實施例2-1中將藉二氧化矽凝膠管柱層析溶 出之溶出液供予TLC(薄層層析）分析的結果。 152 200906315 【主要元件符號說明】 (無） 153Demiglace Flavor Seasoning 1.00 Pork Seasoning 0.50 Meat bouillon Base Flavor 1.00 Potato 7.00 10 Carrot 5.00 Beef 10.00 ZK: _ Total Residue lOO.OOKg Example 4-G-2 Sterilization Bag Curry 15 In addition to implementation In Example 1-1, the extract obtained by using 75Et of 1-2 dried extract of 固态.〇5Kg was used to replace the equol-containing soybean hypocotyl fermentate, and was the same as the above-mentioned Example 4-G-1. Conditions for the manufacture of sterilized bags and coffee garnishes Example 4-G-3 Sterilization bag of saponin 20 According to the method, the bactericidal bag of the following composition is manufactured. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Light soy sauce 72.00 Wine 48.00 Salt 4.00 141 200906315 5 10 L-glutamate 1.30 Nucleic acid seasoning 0.07 Chicken powder 1.00 Sugar 7.00 Water 209.00 Pork 200.00 Flowering stick 120.00 Shrimp 80.00 Scallion (shiitake) 70.00 Boiled bamboo shoots 180.00 Carrot 80.00 Artichoke 240.00 Example 4-G-4 Sterilization bag Chinese saponin 15 20 In addition to the extraction obtained with 75Et in Example 1-1 The sterilized bag of cinnabarin was produced under the same conditions as in the above-mentioned Example 4-G-3 except that the solid extract of the liquid 1-2 was dried up to 5 kg to replace the equol-containing soybean hypocotyl fermentate. Example 4-H Edible processed meats The specific examples of the edible processed meats of the present invention are listed below. Example 4 - H-1 Sausage Sausages were prepared according to the general manufacturing method of sausages using the following constituent materials. Meat mink 5mm minced 70.00 (Kg) 142 200906315 Lard 5mm minced ice water 10.00 20.00 Total lOO.OOKg Auxiliary material 5 Soybean hypocotyl ferment containing equol (Reference 1-3) 〇.40 (Kg) salt 1.70 sodium citrate 0.012 polymeric phosphate 0.30 L-sodium ascorbate 1.00 10 casein sodium 0.50 white pepper powder 0.10 potassium sorbate 0.20 amino acid seasoning 0.30 synthetic spice 0.50 15 Example 4-H-2 Sausage In addition to the above-mentioned Example 4-H, in addition to the solid extract of 萃取.〇5Kg in which the extract 1-2 obtained by using 75Et in Example 1-1 was used instead of the equol-containing soybean hypocotyl fermentate. -1 produces sausages under the same conditions. Example 4 - H-3 Smoked Sausage 20 The meat portion and seasoning of the following composition were mixed in a ratio of 18:1, and the smoked sausage was manufactured in accordance with the general manufacturing method of the smoked sausage. Meat Pork Feet 85.00 (Kg) Whole Egg 4.00 143 200906315 Milk 3.00 Ice Water Seasoning 7.00 Soybean Hypocotyl Fermentation Containing Equol (Reference Example 1-3) 0.40 (Kg) 5 Salt 24.00 Amino Acid Seasoning Material 3.20 Spice 8.85 Broken Onion 18.00 Sugar 10 Example 4-H-4 Smoked Sausage 48.00 In addition to the solid extract of 萃取.〇5Kg after the use of 75Et of extract 1-2 obtained in Example 1-1 Smoked sausages were produced under the same conditions as in the above Example 4-H-3 except for the equol-containing soybean hypocotyl fermentate. Example 4-H-5 Burger Meat 15 The hamburger meat of the following composition was produced according to the method. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0_40 (parts by weight) bovine mixed meat 45.00 porridge 9.00 onion 12.00 20 whole egg 5.00 granulated soy protein 9.00 cool protein sodium 5.00 carrageenan 1.50 hedgehog Bean gum 0.50 144 200906315 Spice 0.30 Yeast extract 0.20 Salt 1.00 Sugar 1.20 5 Water-soluble dietary fiber 1.00 ZK: _ total residue 100.00 parts by weight Example 4-Η-6 Burger meat is obtained in addition to 75Et used in Example 1-1 The hamburger meat was produced under the same conditions as in the above Example 4-H-5, except that 0.05 parts by weight of the solid extract of the extract 1-2 was dried over 10 to replace the soybean-containing hypocotyl fermentate. Example 4-1 Mixed Foods Specific examples of the production examples of the combined foods of the present invention are listed below. 15 Example 4-1-1 Fish plate The fish plate was prepared according to the general manufacturing method of the fish plate using the following constituent materials. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) Fish meat 50.00 20 Salt 1.50 Amino acid seasoning 0.30 Glycine 0.50 Anhydrous sodium acetate 0.30 Potato starch 8.00 145 200906315 Wei Lin 1.00 Ice water _Residue total lOO.OOKg Example 4-1-2 Fish plate 5 In addition to the use of the extract obtained in Example 1-1 using 75Et of the extract 1-2, the dried solid extract 〇. 5Kg to replace the female A fish plate was produced under the same conditions as in the above Example 4-1-1 except for the soybean hypocotyl fermented product of p-phenol. Example 4-J Egg processed foods Specific examples of the production examples of the egg processed food of the present invention are given below. 10 Example 4-J-1 Thick omelet The thick omelet was prepared according to the general manufacturing method of the thick omelet using the raw materials of the following composition. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) Whole egg 70.00 15 Salt 0.40 Starch 2.00 Shang Tang 2.00 Sugar 1.20 Sodium acetate 1.00 20 Water residue total 100.00 parts by weight Example 4- J-2 thick omelet in addition to the 0.05% by weight of the solid extract after extracting 1-2 of the extract 1-2 obtained in Example 1-1 to replace the equol-containing soybean hypocotyl 146 200906315 fermented product, A thick omelet was produced under the same conditions as in the above Example 4-J-1. Example 4-K Canned or Canned Foods Hereinafter, specific examples of the production of canned or canned foods of the present invention will be given. 5 Example 4-K-1 Citrus Cans The syrup and citrus pulp of the following composition were sealed to obtain canned citrus. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) Sugar 10.00 Fructose glucose liquid sugar 10.00 10 Citric acid 0.25 Trisodium citrate 0.10 ^_Residual total 100.00 parts by weight Example 4 -K-2 Citrus Can 15 In addition to 0.05 parts by weight of the solid extract obtained by using 75E of the extract 1-2 obtained in Example 1-1, instead of the equol-containing soybean hypocotyl fermentate, Citrus cans were made under the same conditions as in the previous Example 4-K-1. Example 4-L Bread 20 Hereinafter, specific examples of the production of the bread of the present invention will be given. Example 4 - L-1 Toast The toast was made using the raw material of the following composition. Medium-high-gluten flour 70.00 (Kg) 147 200906315 Yeast activator (yeast-food) 0.10 Live yeast 2.00 Water 40.00 This pinch 5 equol-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (Kg) high Flour flour 30.00 Sugar 3.00 Salt 2.00 Skim milk powder 2.00 10 Oil 5.00 Water 25.00 Example 4-L-2 Toast In addition to the use of the extract obtained in Example 1-1 using 75Et of extract 1-2 dried solid extract 〇 Toast was prepared under the same conditions as in the above Example 4-L-1 except that 5 kg of equol was used instead of the equol-containing soybean hypocotyl fermentation. Example 4 - M cold-boiled snacks Hereinafter, specific examples of the production of the frozen snacks of the present invention will be given. Paste Example 4-M-1 Ice Cream Manufactured ice cream of the following composition. 20 Soybean hypocotyl ferment containing equol (Reference 1-3) 0.40 (parts by weight) Fresh cream 8.80 Cream 5.00 Maltose 18.00 Skim milk powder 8.50 148 200906315 Trehalose 7.00 Sugar 5.00 Stabilizer 0.20 Perfume 0.15 Total water residue 100.00 parts by weight of Example 4-M-2 In addition to the use of the extract obtained in Example 1-1 using 75Et of the extract 1-2 dried extract, 0.05 parts by weight of the solid extract to replace the equol-containing soybean hypocotyl In addition to the fermentate, ice cream was produced under the same conditions as in the above Example 4-M-1. Example 4-N Soybean Processed Foods Hereinafter, specific examples of the soybean processed food production examples of the present invention will be described. Example 4-N-1 Natto 15 99.8 parts by weight of steamed soybeans were mixed with 0. 4 parts by weight of equol-containing soybean hypocotyl fermentate (Reference Example 1-3), and natto was inoculated to ferment to obtain natto. . Example 4 - Ν-2 natto In addition to 0.05 parts by weight of the solid extract after extracting 1-2 dry extract 20 obtained using 75Et in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, Natto was produced under the same conditions as in the above Example 4-N-1. Example 4 - N-3 Tofu The following composition of tofu was produced. Soybean hypocotyl fermented product containing equol (Reference Example 1-3) 0.40 (parts by weight) 149 200906315 99.30 0.20 0.10 Soymilk Magnesium chloride Potassium sorbate Total 100.00 parts by weight. 5 Example 4-N-4 Tofu In addition to 0.05 parts by weight of the solid extract obtained by using 75E of the extract 1-2 obtained in Example 1-1, instead of the equol-containing soybean hypocotyl fermentate, The standing rot was produced under the same conditions as the above-mentioned Example 4-N-3. Example 4-0 Rice Food 10 Hereinafter, specific examples of the production example of the rice food of the present invention will be given. Example 4-0-1 Porridge The porridge was prepared according to the general manufacturing method of porridge using the following constituent materials. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) Rice 180.00 15 Salt 1.00 ^_900.00 Example 4-0-2 Porridge was obtained by using 75Et in Example 1-1. The porridge was produced under the same conditions as in the above Example 4-0-1, except that the extract of the extract 1-2 was dried in an amount of 0.05 parts by weight to replace the equol-containing soybean hypocotyl 20 ferment. Example 4-0-3 Chestnut glutinous rice The chestnut glutinous rice was prepared by a general production method using the raw materials of the following composition. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 4.00 (parts by weight) 150 200906315 Example 4-0-4 Chestnut glutinous rice 10 15 glutinous rice 1000.00 Shelled chestnut 300.00 Noodle soup 230.00 Black sesame 30.00 Saline 8.00 In addition to 0.5 parts by weight of the solid extract obtained by using 75Et of the extract 1-2 obtained in Example 1-1 to replace the soybean hypocotyl fermented product containing the esculent, with the above Example 4-0 -3 The same conditions produce chestnut glutinous rice. Example 4-P Soup The following is a specific example of the production example of the soup of the present invention. Example 4-P-1 Corn Cream Soup Corn cream soup was prepared according to the general method of the soup using the raw materials of the following composition. Erythromycin-containing soybean hypocotyl ferment (Reference Example 1-3) 0.40 (parts by weight) 20 Milk 180.00 Corn 190.00 Clear broth (consommi) 20.00 Salad oil 15.00 Salt amount of pepper appropriate amount 380.00 Example 4-P-2 Corn cream soup 151 200906315 In addition to the 0.05 parts by weight of the solid extract obtained by using the extraction solution ι_2 obtained by using 75Et in Example 1-1, in place of the equol-containing soybean hypocotyl fermentate, The corn cream soup was made under the same conditions as in Example 4-P-1. 5 [Simplified description of the drawings] Fig. 1 shows the results (electropherogram) of the total protein contained in the equol-containing soybean hypocotyls, soybean cotyledons and soybean hypocotyls obtained in Reference Example M. Fig. 2 shows the results (electropherogram) of the main allergens (Gym4, 10Gm30K, Gm28K) contained in the equol-containing soybean hypocotyls, the soybean cotyledons and the soybean hypocotyls obtained in Reference Example M. Figure 3 shows the main allergens (7S globulin mix, oily protein (Oleosins), trypsin inhibitor) contained in the equol-containing soybean hypocotyl fermentate, soybean cotyledon and soybean hypocotyls obtained in Reference Example M. The result of trypsin inhibitor)) (electropherogram). 15 Fig. 4 shows the results of analyzing the equol contents of the extracts 1_2 and 2-2 by TLC in Example 1. Fig. 5 shows the results of analyzing the saponin contents of the extracts 1-2 and 2-2 by TLC in Example 1. Fig. 6 shows the results of analyzing the equol content of the extract 1-2 and the residue 2 20 by TLC in Example 1-1. Fig. 7 shows the results of analyzing the saponin contents of Extract 1-2 and Residue 2 by TLC in Example 1-1. Fig. 8 shows the results of analysis of TLC (thin layer chromatography) analysis of the eluate eluted by the cerium oxide gel column chromatography in Example 2-1. 152 200906315 [Description of main component symbols] (none) 153

Claims (1)

200906315 十、申請專利範圍： 1. 一種含雌馬酚萃取物之製造方法，包含：第1-1步驟，係 使用乙醇水溶液作為萃取溶劑，將含雌馬酚之大豆胚軸 發酵物進行萃取處理，並回收萃取液。 5 2.如申請專利範圍第1項之製造方法，其更包含第1-2步 驟，即：從第1-1步驟所得萃取液去除萃取溶劑後，使用 乙醇作為萃取溶劑將其進行萃取處理，再回收萃取液。 3.如申請專利範圍第1項之製造方法，其中該第1-1步驟所 使用之乙醇水溶液中的乙醇濃度為20〜98容量％。 10 4.如申請專利範圍第1〜3項中任一項之製造方法，其中該 含雌馬酚之大豆胚軸發酵物係以可產生雌馬酚之微生 物使大豆胚軸發酵而得者。 5. —種含雌馬酚之萃取物，係使用乙醇水溶液作為萃取溶 劑，將含雌馬酚之大豆胚軸發酵物進行萃取處理而獲得 15 者。 6. —種含雌馬酚之萃取物，係對含雌馬酚之大豆胚軸發酵 物依序實施使用乙醇水溶液之萃取處理及使用乙醇之 萃取處理而獲得者。 7. —種含雌馬酚之萃取物，係以如申請專利範圍第1〜4項 20 中任一項之製造方法而製得者。 8. —種含雌馬酚萃取物之製造方法，係從含雌馬酚之大豆 胚軸發酵物製造含雌馬酚之萃取物者，其包含下述第 II-1步驟及第II-2步驟： 第II-1步驟，係使含雌馬酚之大豆胚軸發酵物懸浮於 154 200906315 水中，加入酸而調整至pH2〜6，再回收不溶物；及 第11 - 2步驟，係使用乙醇水溶液作為萃取溶劑對前述 第II-1步驟所得之不溶物進行萃取處理，並回收萃取液 者。 5 9.如申請專利範圍第8項之製造方法，其中該第II-1步驟所 使用之酸為乙酸。 10. —種含雌馬酚之萃取物，係以如申請專利範圍第8或9項 之製造方法製得者。 11. 一種雌馬紛之純化方法，係由雌馬紛含有物純化出雌馬 10 酚者，包含下述第1-1步驟〜第1-5步驟： 第1-1步驟，係使用乙醇水溶液作為萃取溶劑，將雌 馬酚含有物進行萃取處理，並回收萃取液； 第1-2步驟，係從該第1-1步驟所得萃取液去除溶劑 後，使用乙醇作為萃取溶劑對所得殘留物進行萃取處 15 理，並回收萃取液； 第1-3步驟，係從該第1-2步驟所得萃取液去除溶劑 後，使所得殘留物懸浮於己烷中，並回收不溶物； 第1-4步驟，係使用己烷及醚之混合液作為萃取溶 劑，對前述第1-3步驟所得不溶物進行萃取處理，並回收 20 萃取液；及 第1-5步驟，係將該第1-4步驟所得萃取液進行二氧 化石夕凝膠層析，並回收含雌馬盼之分液者。 12. 如申請專利範圍第11項之純化方法，其中該雌馬酚含有 物為含雌馬酌·之發酵物。 155 200906315 13. 如申請專利範圍第12項之純化方法，其中該含雌馬酚之 發酵物係含雌馬酌之大豆胚軸發酵物，其係以產生雌馬 紛之微生物使大豆胚軸發酵而獲得者。 14. 如申請專利範圍第11〜13項中任一項之純化方法，其中 5 該第1-4步驟所使用之萃取溶劑為己烷與二乙醚之混合 液。 15. 如申請專利範圍第11〜14項中任一項之純化方法，其中 該第1-4步驟所使用之萃取溶劑為己烷與醚係10 : 90〜20 : 80容量比之混合液。 10 16.如申請專利範圍第11〜15項中任一項之純化方法，其中 該第1-1步驟所使用之乙醇水溶液中的乙醇濃度為 20〜98容量％。 17. —種雖馬酌·之純化方法，係由雖馬紛含有物純化出雕馬 酚者，含有下述第2-1步驟〜第2-3步驟： 15 第2-1步驟，係使用選自於由乙酸乙酯、乙醇、丙酮、 二噁烷、乙腈、二乙醚及甲苯所構成群組中之至少1種 有機溶劑或其含水有機溶劑作為萃取溶劑，對雌馬酚含 有物進行萃取處理，再濃縮所得萃取液； 第2-2步驟，係將該第2-1步驟所得雌馬酚濃縮物供 20 予二氧化矽凝膠管柱層析，而獲得含雌馬酚之分液者； 及 第2-3步驟，係從該第2-2步驟所得含雌馬酚之分液 去除溶劑，對所得殘留物進行再結晶處理，並回收析出 之結晶者。 156 200906315 18. 如申請專利範圍第17項之純化方法，其係於前述第2-3 步驟中，使用乙酸乙酯與己烷之混合液進行再結晶處理 後，更進行使用乙醇水溶液之再結晶處理。 19. 一種食品素材，係由含雌馬酚之大豆胚軸發酵物或其萃 5 取物分散於可可塊(cacao mass)而成者。 20. 如申請專利範圍第19項之食品素材，其中相對於含雌馬 酚之大豆胚軸發酵物或其萃取物100重量份，所含可可 塊係占10〜2000重量份之比率。 21. 如申請專利範圍第19或20之食品素材，其中該含雌馬酚 10 之大豆胚轴發酵物係以產生雌馬盼之微生物使大豆胚 軸發酵而得者。 22. 如申請專利範圍第21項之食品素材，其中前述產生雌馬 酚之微生物係一具有將木質素黃酮類資化而產生雌馬 酚之能力的微生物，且該木質素黃酮類係選自於由木質 15 素黃酮配糖體、木質素黃酮及二氫木質素黃酮所構成群 組中之至少1種。 23. 如申請專利範圍第19〜22項中任一項之食品素材，其係 呈粒狀、片狀或板狀。 24. —種含雌馬酚之食品，含有如申請專利範圍第19〜23項 20 中任一項之食品素材。 25. 如申請專利範圍第24項之含雌馬酚之食品，其係一烘 烤點心。 26. —種食品，含有含雌馬酚之大豆胚軸發酵物或其萃取 物。 157 200906315 七、指定代表囷： (一） 本案指定代表圖為：第（4 )圖。 (二) 本代表圖之元件符號簡單說明： - (無） 八、本案若有化學式時，請揭示最能顯示發明特徵的化學式：200906315 X. Patent application scope: 1. A method for producing equol-containing extract, comprising: step 1-1, using an aqueous solution of ethanol as an extraction solvent, and extracting the fermentation product of equol-containing soybean hypocotyls And recover the extract. 5 2. The manufacturing method of claim 1, further comprising the step 1-2, that is, after extracting the extraction solvent from the extract obtained in the first step, extracting the solvent using ethanol as an extraction solvent, The extract is recovered again. 3. The production method according to the first aspect of the invention, wherein the ethanol concentration in the aqueous ethanol solution used in the step 1-1 is 20 to 98% by volume. The manufacturing method according to any one of claims 1 to 3, wherein the equol-containing soybean hypocotyl fermented product is obtained by fermenting soybean hypocotyls with equol-producing microorganisms. 5. An extract containing equol, which is obtained by extracting an equol-containing soybean hypocotyl fermentate using an aqueous ethanol solution as an extraction solvent. 6. An extract containing equol, which is obtained by subjecting an equol-containing soybean hypocotyl fermentate to an extraction treatment using an aqueous ethanol solution and an extraction treatment using ethanol. 7. An extract containing equol, which is obtained by the production method according to any one of claims 1 to 4 of the patent application. 8. A method for producing an equol-containing extract, which comprises producing an equol-containing extract from an equol-containing soybean hypocotyl ferment, which comprises the following steps II-1 and II-2 Step: Step II-1, suspending the equol-containing soybean hypocotyl fermented product in 154 200906315 water, adding acid to adjust pH to 2-6, and recovering insoluble matter; and step 11-2, using ethanol The aqueous solution is used as an extraction solvent to extract the insoluble matter obtained in the above step II-1, and the extract is recovered. 5. The method of manufacture of claim 8, wherein the acid used in the step II-1 is acetic acid. An extract containing equol, which is obtained by the production method of claim 8 or 9. 11. A method for purifying a female horse by purifying a female horse 10 phenol from a female horse, comprising the following steps 1-1 to 1-5: Step 1-1, using an aqueous ethanol solution As an extraction solvent, the equol content is subjected to extraction treatment, and the extract is recovered. In the first step, the solvent is removed from the extract obtained in the first step 1-1, and the residue is subjected to using ethanol as an extraction solvent. The extraction is carried out, and the extract is recovered; in the first to third steps, after removing the solvent from the extract obtained in the first step 1-2, the obtained residue is suspended in hexane, and the insoluble matter is recovered; The step of extracting the insoluble matter obtained in the above steps 1-3 by using a mixture of hexane and ether as an extraction solvent, and recovering the 20 extract; and the steps 1-5, the steps 1-4 The obtained extract was subjected to silica dioxide gel chromatography, and the fraction containing the female horse was recovered. 12. The purification method according to claim 11, wherein the equol content is a fermented product containing a female horse. 155 200906315 13. The purification method according to claim 12, wherein the equol-containing fermented product comprises a female horse-prepared soybean hypocotyl fermented product, which is produced by producing a female equine microorganism to ferment soybean hypocotyls And the winner. 14. The purification method according to any one of claims 11 to 13, wherein the extraction solvent used in the steps 1-4 is a mixture of hexane and diethyl ether. 15. The purification method according to any one of claims 11 to 14, wherein the extraction solvent used in the steps 1-4 is a mixture of hexane and ether 10:90 to 20:80 capacity ratio. The purification method according to any one of claims 11 to 15, wherein the ethanol concentration in the aqueous ethanol solution used in the step 1-1 is 20 to 98% by volume. 17. A method for purifying a horse, according to the method of purifying the carrageenan, containing the following steps 2-1 to 2-3: 15 step 2-1, using The equol content is extracted by selecting at least one organic solvent selected from the group consisting of ethyl acetate, ethanol, acetone, dioxane, acetonitrile, diethyl ether and toluene or an aqueous organic solvent thereof as an extraction solvent After the treatment, the obtained extract is further concentrated; in step 2-2, the equol concentrate obtained in the step 2-1 is subjected to 20 column chromatography on cerium oxide gel to obtain a liquid containing equol. And in the step 2-3, the solvent is removed from the equol-containing liquid obtained in the second step 2-2, and the obtained residue is subjected to recrystallization treatment, and the precipitated crystals are recovered. 156 200906315 18. The purification method according to claim 17 of the patent application, in the above step 2-3, after recrystallization treatment using a mixture of ethyl acetate and hexane, further recrystallization using an aqueous ethanol solution deal with. 19. A food material obtained by dispersing a equol-containing soybean hypocotyl fermentate or a extract thereof in a cacao mass. 20. The food material according to claim 19, wherein the cocoa mass is contained in a ratio of 10 to 2000 parts by weight relative to 100 parts by weight of the equol-containing soybean hypocotyl fermentate or the extract thereof. 21. The food material of claim 19 or 20, wherein the equol-10-containing soybean hypocotyl fermentate is obtained by fermenting a soybean embryo shaft with a microorganism that produces an esculent. 22. The food material of claim 21, wherein the microorganism producing equol is a microorganism having the ability to produce equol by lignin flavonoids, and the lignin flavonoid is selected from the group consisting of It is at least one selected from the group consisting of lignin 15 flavonoid glycoside, lignin flavone, and dihydrolignin flavonoid. 23. The food material of any one of claims 19 to 22, which is in the form of a granule, a sheet or a plate. 24. A food containing equol, comprising the food material of any one of claims 19 to 23 of claim patent. 25. For the equol-containing food of claim 24, it is a baked snack. 26. A food product comprising an equol-containing soybean hypocotyl fermentate or an extract thereof. 157 200906315 VII. Designation of representatives: (1) The representative representative of the case is: (4). (2) A brief description of the symbol of the representative figure: - (none) 8. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention:
TW097122135A 2007-06-13 2008-06-13 An extract containing equol (EQUOL), a method for producing the same, an equol extraction method and an equol-containing food TWI473572B (en)

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