[go: up one dir, main page]

TW200517656A - Apparatus and method detecting nucleic acid in biological samples - Google Patents

Apparatus and method detecting nucleic acid in biological samples

Info

Publication number
TW200517656A
TW200517656A TW093131325A TW93131325A TW200517656A TW 200517656 A TW200517656 A TW 200517656A TW 093131325 A TW093131325 A TW 093131325A TW 93131325 A TW93131325 A TW 93131325A TW 200517656 A TW200517656 A TW 200517656A
Authority
TW
Taiwan
Prior art keywords
nucleic acid
biological samples
detecting nucleic
method detecting
field
Prior art date
Application number
TW093131325A
Other languages
Chinese (zh)
Other versions
TWI360655B (en
Inventor
Cheung-Hoi Yu
Lok-Ting Lau
Ka-Wai Wong
Original Assignee
Hong Kong Dna Chips Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hong Kong Dna Chips Ltd filed Critical Hong Kong Dna Chips Ltd
Publication of TW200517656A publication Critical patent/TW200517656A/en
Application granted granted Critical
Publication of TWI360655B publication Critical patent/TWI360655B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

There are disclosed apparatus and methods for the field-assisted acceleration of biological processes involving charged entities, including in particular the detection of target DNA in a biological sample. A reaction cell is provided with a dielectric surface, and a field is generated by inducing charge-separation in the dielectric material by applying a potential to an electrode in contact with the dielectric material.
TW093131325A 2003-10-16 2004-10-15 Apparatus and methods for detecting nucleic acid i TWI360655B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/686,252 US7390622B2 (en) 2003-10-16 2003-10-16 Apparatus and methods for detecting nucleic acid in biological samples

Publications (2)

Publication Number Publication Date
TW200517656A true TW200517656A (en) 2005-06-01
TWI360655B TWI360655B (en) 2012-03-21

Family

ID=34465492

Family Applications (1)

Application Number Title Priority Date Filing Date
TW093131325A TWI360655B (en) 2003-10-16 2004-10-15 Apparatus and methods for detecting nucleic acid i

Country Status (13)

Country Link
US (3) US7390622B2 (en)
EP (2) EP1673476B1 (en)
JP (2) JP5248017B2 (en)
CN (1) CN1867681B (en)
AU (1) AU2004282235B2 (en)
BR (1) BRPI0415342A (en)
CA (1) CA2542518C (en)
MX (1) MXPA06004205A (en)
MY (2) MY144038A (en)
RU (1) RU2359038C2 (en)
SG (1) SG147452A1 (en)
TW (1) TWI360655B (en)
WO (1) WO2005038048A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7390622B2 (en) * 2003-10-16 2008-06-24 Hai Kang Life Corporation Limited Apparatus and methods for detecting nucleic acid in biological samples
US8906609B1 (en) 2005-09-26 2014-12-09 Arrowhead Center, Inc. Label-free biomolecule sensor based on surface charge modulated ionic conductance
KR100723427B1 (en) * 2006-05-12 2007-05-30 삼성전자주식회사 Apparatus and method for printing biomolecule droplets on a substrate
US20080221805A1 (en) * 2007-03-09 2008-09-11 David Richard Andrews Multi-channel lock-in amplifier system and method
ES2307430B1 (en) * 2007-05-09 2009-10-20 Consejo Superior De Investigaciones Cientificas BIOSENSOR AND ITS APPLICATIONS.
US8277628B2 (en) * 2007-07-13 2012-10-02 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
KR100969671B1 (en) * 2008-03-28 2010-07-14 디지탈 지노믹스(주) High sensitivity biosensor, biochip comprising same and method for manufacturing same
WO2011108540A1 (en) 2010-03-03 2011-09-09 国立大学法人大阪大学 Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
CA2877888C (en) 2012-06-28 2016-02-02 Siemens Healthcare Diagnostics Inc. Reader device and method of signal amplification
EP2954315A2 (en) * 2013-02-07 2015-12-16 Koninklijke Philips N.V. Processing of nucleotide sequences
US9151759B2 (en) * 2013-05-06 2015-10-06 Research Foundation Of The City University Of New York Method for detecting cells with elastic cell membranes
WO2015042200A1 (en) 2013-09-18 2015-03-26 Osaka University Biomolecule sequencing devices, systems and methods
JP2015077652A (en) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 Nano-gap electrode and method for manufacturing same
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
WO2015170782A1 (en) 2014-05-08 2015-11-12 Osaka University Devices, systems and methods for linearization of polymers
US9533278B2 (en) 2014-08-08 2017-01-03 Applied Materials, Inc. Patterned deposition of liquid films for biomedical devices
US10379101B2 (en) * 2015-11-23 2019-08-13 Hai Kang Life Corporation Limited Apparatus for detection of biomolecules and its fabrication
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
KR20180123062A (en) * 2016-02-29 2018-11-14 이리디아, 인크. Method, composition, and apparatus for information storage
ES2877193T3 (en) 2016-04-27 2021-11-16 Quantum Biosystems Inc Systems and methods for the measurement and sequencing of biomolecules
CN108130273B (en) * 2016-12-01 2021-10-12 京东方科技集团股份有限公司 Detection substrate, manufacturing method thereof and method for detecting nucleic acid
RU2769999C2 (en) * 2017-12-28 2022-04-14 Адор Диагностикс С.Р.Л Electrophoretic chip and method for fast detection of the presence of target nucleic acid molecules
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1337173C (en) * 1989-04-28 1995-10-03 Westaim Biomedical Corp. Thin film diagnostic device
RU2032908C1 (en) * 1991-04-26 1995-04-10 Институт геохимии и аналитической химии им.В.И.Вернадского РАН Device for determination of biologically active compounds in biological fluids and process of manufacture of sensitive element
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US6306348B1 (en) * 1993-11-01 2001-10-23 Nanogen, Inc. Inorganic permeation layer for micro-electric device
JPH08154656A (en) * 1994-12-05 1996-06-18 Nikon Corp Nucleic acid hybridization test method, nucleic acid hybridization test substrate and nucleic acid hybridization test device
US5605622A (en) * 1995-07-18 1997-02-25 Ferraro; Michael J. Swimming pool vacuum system
WO1998046797A1 (en) * 1997-04-16 1998-10-22 Immunological Associates Of Denver Nucleic acid archiving
US20040053290A1 (en) * 2000-01-11 2004-03-18 Terbrueggen Robert Henry Devices and methods for biochip multiplexing
WO2002043864A2 (en) * 2000-11-03 2002-06-06 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
US20020090649A1 (en) * 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
JP2003517149A (en) * 1999-12-15 2003-05-20 モトローラ・インコーポレイテッド High density biochip array with row and column addressability
US6458000B2 (en) * 1999-12-30 2002-10-01 Thomas & Betts International, Inc. Power connector ground polarization insert and connector used therewith
US6716620B2 (en) * 2000-04-17 2004-04-06 Purdue Research Foundation Biosensor and related method
US7306924B2 (en) * 2000-04-17 2007-12-11 Purdue Research Foundation Biosensor and related method
AU2002230381A1 (en) * 2000-08-31 2002-04-22 Motorola, Inc. High density column and row addressable electrode arrays
JP4193345B2 (en) * 2000-09-07 2008-12-10 横河電機株式会社 Measuring device for measuring gene sequences of biopolymers
JP3758183B2 (en) * 2001-11-08 2006-03-22 横河電機株式会社 Biochip and gene sequence measuring apparatus using the same
US7153687B2 (en) * 2002-08-13 2006-12-26 Hong Kong Dna Chips Limited Apparatus and methods for detecting DNA in biological samples
CN1186627C (en) 2003-02-28 2005-01-26 北京青鸟元芯微系统科技有限责任公司 Chip of low power dissipation chemical gas sensor and its preparing method
JP4482856B2 (en) * 2003-08-29 2010-06-16 セイコーエプソン株式会社 Method for detecting target substance in sample, sensor substrate, and detection kit
JP4281479B2 (en) * 2003-09-05 2009-06-17 ソニー株式会社 Bioassay substrate for detecting an interaction between an electrode unit and a substance using the electrode unit
JP4403376B2 (en) * 2003-10-09 2010-01-27 ソニー株式会社 Detecting interaction between substances, bioassay substrate provided with the detection unit, and method for supplying aqueous solution to the detection unit
US7390622B2 (en) * 2003-10-16 2008-06-24 Hai Kang Life Corporation Limited Apparatus and methods for detecting nucleic acid in biological samples

Also Published As

Publication number Publication date
AU2004282235B2 (en) 2011-09-08
WO2005038048A1 (en) 2005-04-28
MY139466A (en) 2009-10-30
RU2359038C2 (en) 2009-06-20
TWI360655B (en) 2012-03-21
SG147452A1 (en) 2008-11-28
AU2004282235A1 (en) 2005-04-28
MY144038A (en) 2011-07-29
EP2554683A1 (en) 2013-02-06
BRPI0415342A (en) 2006-12-05
US20120010093A1 (en) 2012-01-12
US7390622B2 (en) 2008-06-24
EP1673476A4 (en) 2010-01-06
US20050084865A1 (en) 2005-04-21
EP1673476A1 (en) 2006-06-28
CN1867681A (en) 2006-11-22
JP5248017B2 (en) 2013-07-31
US7888109B2 (en) 2011-02-15
CN1867681B (en) 2010-12-08
US20080242562A1 (en) 2008-10-02
JP2007508554A (en) 2007-04-05
JP2013140168A (en) 2013-07-18
HK1098177A1 (en) 2007-07-13
CA2542518A1 (en) 2005-04-28
CA2542518C (en) 2012-05-29
RU2006111450A (en) 2007-10-20
MXPA06004205A (en) 2007-02-16
EP1673476B1 (en) 2013-06-12

Similar Documents

Publication Publication Date Title
TW200517656A (en) Apparatus and method detecting nucleic acid in biological samples
KR101963462B1 (en) Systems and methods for automated reusable parallel biological reactions
WO2007061943A8 (en) Portable preparation, analysis, and detection apparatus for nucleic acid processing
US9580742B2 (en) Diagnostic and sample preparation devices and methods
MXPA06001914A (en) Method and apparatus for assay of electrochemical properties.
EP4056702A3 (en) Scalable bio-element analysis
EA201071134A1 (en) DNA ANALYSIS BY CAPILLARY ELECTROPHORESIS
Cui et al. Electrochemical biosensing for E. coli detection based on triple helix DNA inhibition of CRISPR/Cas12a cleavage activity
ATE455180T1 (en) BIOCHIP ELECTROPORATION DEVICE WITH MULTIPLE POSITIONS FOR SINGLE CELL ELECTROPORATION
GB201015803D0 (en) Methods and apparatus for amplifying nucleic acids
CN104931570A (en) Preparation method and application of electrochemical sensor for heavy metal ions based on aptamers
Yue et al. A triple amplification strategy using GR-5 DNAzyme as a signal medium for ultrasensitive detection of trace Pb2+ based on CRISPR/Cas12a empowered electrochemical biosensor
CN102636553A (en) Paper-based electrospray ion mobility spectrometry analysis method
Liu et al. Separation of biogenic amines by micellar electrokinetic chromatography with on-line chemiluminescence detection
US20160178488A1 (en) Microfluidic transducer
Wei et al. A novel multiplex signal amplification strategy based on microchip electrophoresis platform for the improved separation and detection of microRNAs
WO2004104211A3 (en) Method for detecting toxic and non-toxic cyanobacteria
CN103060429A (en) Flight mass spectrum biochip for innate potential assessment and its detection method
WO2005005952A3 (en) Electrocatalytic nucleic acid hybridization detection
US20070240990A1 (en) Analysis
ATE386263T1 (en) METHOD AND APPARATUS FOR ANALYZING CHEMICAL OR BIOLOGICAL SAMPLES
WO2022046546A1 (en) Methods and apparatuses for detecting biomolecules
Zhao et al. Electrical potential-assisted DNA-RNA hybridization for rapid microRNA extraction
Paleček et al. From DC polarographic presodium wave of proteins to electrochemistry of biomacromolecules
Ly et al. Detection of Helicobacter pylori DNA in preliminary stage gastric cancer cells

Legal Events

Date Code Title Description
MM4A Annulment or lapse of patent due to non-payment of fees