TR202008828A2 - METHOD OF OBTAINING HIPPOPHAE RHAMNOIDES EXTRACT ENCAPSULATED WITH LIPOSOMAL TECHNOLOGY FOR USE IN COSMETIC FORMULAS - Google Patents

Abstract

This invention relates to formulations of regenerative personal care products prepared with the extract of Sea buckthorn encapsulated using liposomal technology and their production method. Microfludizer system is used to encapsulate wild sequin oil with phospholipid. The formulation also contains unsaturated fatty acids such as Palmito-oleic acid (omega-7) and &#978#&-linolenic acid (omega-6) for skin regeneration and repair.

Description

DESCRIPTION LIPOSOMAL FOR USE IN COSMETIC FORMULAS WILD IGDE (HIPPOPHAE) ENCAPSULATED WITH TECHNOLOGY RHAMNOIDES) EXTRACT METHOD Technical Area This invention is based on the extract of Wild igdese encapsulated using lipozoirial technology. With personal care product formulations with regenerative effect, prepared with Prior Art Wild larch (Hgvpophae rhamnoides) oil is the most abundant in terms of omega-7 palmitoleic acid. It is one of the richest sources, contains carotenoids and tocopherols. Because from its seeds, fruits and vegetables in cosmetic formulas and food supplements. The extract obtained from the leaves is used in its natural form [1], [2].

Fixed and essential oils (EO) obtained from plants are natural, complex compounds.

They are found in the structure of aromatic plants as secondary metabolites.

It can be synthesized by all plant organs such as buds, flowers, leaves, stems, branches, seeds, fruits, roots, tree or bark and secretion cells, spaces, ducts, epidermic cells or glandular are found in trichomes. Mono-, sesqui- and di-terpenes, including terpenes, are volatile It is one of the main molecules of oils. These oils are antifungal, antioxidant and bactericidal. It has a wide range due to its biological effects. Therefore, in recent years The use of essential and fixed oils in the food industry and pharmacy is increasing.

However, it is well known that most vegetable oils are not biologically stable.

They are sensitive to oxygen, light and temperature. Some essential oils are affected by oxygen Contains labile functional groups, oxidizing oxygen for alcohols and aldehydes as possible, radiation unsaturated monoterpenic and sesquiterpenic 25418.101 can change the structure of hydrocarbons. In addition, the solubility of oils in water it is weak. For this reason, the encapsulation method determines the solubility and solubility of vegetable oils. increase their bioavailability, at the same time control their release are used to increase their effectiveness. lipid vesicle membrane composition, especially the type of phospholipids, cholesterol content, molar ratio, preparation method and Types liposome size and encapsulation may affect its efficiency. Essential oils can reduce the size of liposomes, It can homogenize liposomal dispersions, increase fluidity and lipid It can reduce the oxidation of the layer [3].

In a study, supercritical carbon dioxide from wild spruce leaves As a result of encapsulation of the extract obtained by the method, antioxidant activity was higher than the extract obtained from the same plants. has been proven [2].

Vegetable oils, medicinal and cosmetic products as a rich source of fatty acids [4], [5], [6], [7], [8], [9]. The fatty acids found in these oils forms a barrier film on the skin that reduces transepidermal water loss (TEWL), thus contributing to maintaining the correct hydration of the epidermis. softens, thus contributing to the continuity of the intracellular structure of the skin [6], [7], Additionally, the effect of oils on skin and human health may vary [4], [5], [6], [7]. For example, lipid deficiency causes the skin to be deprived of an adequate protective layer and causes drying [9].

The reason why vegetable oils are one of the main ingredients in cosmetic products, excess water through the skin by forming a barrier film that mainly covers the epidermis It is the protection against the loss of skin, that is, it provides the moisture of the skin [6], [7], [9]. 25418.101 Triglycerides, which are long chain fatty acids, are essential for the proper functioning of the human body. plays an important role [4], [5], [7], [10]. Vegetable oils, cell membrane components or ecosanoids (ecosanoids: prostaglandins, prostacyclins, thromboxanes, leukotrienes) plays an important role in its biological synthesis [6].

One of the natural glycerides, with a rich chemical composition and unique properties It is obtained by extraction from the fruit or the seeds of the plant [15].

The latest scientific studies, antioxidants, vitamin C, flavonoids, polysaccharides by cold extraction from the fruit of the plant, including polyphenols Many active substances in Y. igde (Hippophaes rhamnoides) extract confirms its existence. Today, both Y. igde (F mcrus Hippophae) fruit and its seeds (Straw Hippaphae) are used only for the food industry. of cosmetic products not only because of its raw material but also because of its benefits for the skin. It is a useful component [15].

Botanical description of Y. igde (Hippophaes rhamnoides): Common Y. igde (Hippophaes rhanmofdes), also known as Siberian pineapple, oleaster It is a thorny shrub (or tree) [15], [19], [20]. It has a flat or sometimes cracked bark. plant Europe, It grows in the Caucasus, Asia, Siberia, China and Tibet [19], [22], [23]. Y. igde late April and blooms in early May. The plant, with silvery hairs underneath It has coated long lanceolate leaves. Bush, many in the form of bunches produces small, green and brown flowers. After the flowering period, September Tasty and nutritious round fruit ripening in the month, usually yellow or orange they turn into fruits. An oily seed with a long groove inside the fruit Igde fruits are bitter and minty, with a delicate aroma similar to that of pineapple [1 51, mg), but also vitamin A with alpha and beta-carotene (up to 60 mg), and 25418.101 Contains a mixture of other carotenoids (up to 180 mg% in total). Moreover, tocopherols in fruits, vitamin E (from 110 to 160 mg), folic acid (to 0.79 mg) Vitamin, catechins and procyanidins, cyclitols, phospholipids, tannins, carbohydrates (galactose, fructose, xylose, approx. 3.9%), organic acids (maleic acid, and geographical location. For example, Y. igde contains 120-315 mg of vitamin C in fresh fruits, while species grown in the Alps Sinemis) is the most abundant vitamin C with ascorbic acid content up to 2500 mg%. It also has a high content of carotenoids that have an effect. Beta-carotene content 40-100% mg and other lycopene, cryptoxanthin, physalien, zeaxanthin. The juice obtained when the fruits are pressed is divided into three layers. top layer is a thick layer of Citrus, the middle layer is a mixture of saturated and unsaturated fatty acids. contains a mixture and the substrate is an oil source used for cosmetic purposes. layer [34], [35], [36], [37]. Two top layers in the production of skin care creams can be processed and used, and the substrate is often used as a syrup in the food industry. used. Today, the highly nutritious components of common Y. igde fruits, adjunctive therapy, for example in the treatment of inflammations, cancers and after chemotherapy Y. igdem bark and leaves for the treatment of diarrhea and dermatological conditions While using, oil that is applied topically or taken orally softens the skin. Chemical composition of ingredients 25418.101 Y. igde oil has a unique fatty acid content compared to other vegetable oils. [45], [46], [47]. In particular, this oil is a component of skin lipids and rare palmitooleic acid that stimulates regenerative processes in the epidermis and wound healing It should be noted that it contains (omega-7). In this way, Y. igdem oil is physiological. activates its functions and reduces scars [48], [49], [50]. orally when used, it supports the treatment of stomach, duodenal and intestinal ulcers, Topically soothes skin burns (sun exposure or radiation therapy). Caused by friction), irritated skin, wounds and trophic skin from friction changes and calms the skin [48], [49], [50]. In addition, Y. igde oil, contains acids and especially so-called PUFA (polyunsaturated fatty acids). contains fatty acids [15], [45], [46], [47]. These include alpha-linolenic acid (omega- numerical symbols General formula Content in Common name Systematic name family Saturated fatty acids Palmitic acid Hexadecanoic acid MCOO C16:0 stearic acid Unsatu rated fatty Palmiioleic acid oleic acid Linoleic acid (LA) o-Linolenic acid y-Iinolenic acid Gondoic acid Octadecanoic acid (2)-9-hexadecenoic acid (2)-9-0ctadecenoic acid (Z,Z)-9,12-0ctadecadienoic (Z,Z,Z)-9,12,15- octadecatrienoic (Z,Z,Z)-6,9,12~ octadecatrienoic (Z)-11-eicosen0ic acid -35 14-18 CH3(CH2)15COO C16H3002 C18H3402 C1BH32O2 C1BH3002 CîßHBÜOZ C18:0 Table 1 Composition of fatty acids in wild igdc oil Such high y-linolenic acid (GLA) content has an impact on the transport of nutrients. has a significant impact. GLA is also a very important component for the skin, because it binds the epidermis cells as an intercellular structural component. Same It is also found in the composition of phospholipids that form cell membranes [17], [18]. y- 25418.101 Linolenic acid increases blood circulation in the skin and as a result, skin structure, removes excess toxins improving appearance and tone. found in Y. igde oil GLA easily penetrates deeper skin layers when converted to prostaglandins. it does. Therefore, GLA effectively protects the skin against infections, counteracts, relieves inflammation and slows down the aging process [51], [52]. Omega- The skin deprived of 6 acids is drier, less elastic and prone to any lesions. becomes sensitive [52]. Linoleic acid, a component of intercellular substance The presence of (omega-6) promotes cellular regeneration and stimulates skin sebaceous glands. regulates its functions [53]. On the other hand, the high content of saturated fatty acids (above 30%) against softening of the epidermis and transepidermal water loss Saturated fatty acids The most common saturated fatty acids in vegetable oils are palmitic, stearic, myristic and are aracidic fatty acids. High stability of oil and resistance to oxidation. they provide [6]. Y. larch oil contains palmitic and stearic acids [6], [15]. These acids are It forms a protective layer on it, which strengthens the effect of a protective barrier.

They provide the elasticity and firmness of the skin and are smoothing and softening. Unsaturated fatty acids This acid group includes fatty acids with double bonds. For the most part, all double bonds are a cis in the configuration [6]. Today, two main classes of unsaturated fatty acids can be distinguished. Monounsaturated FA (omega-9; (9-9, n-9 acids) and polyunsaturated FA (omega-6; (ii-6; n-6 acids). Polyunsaturated FA has at least two in an alkyl chain it has a double bond and 18 carbon atoms [6]. Y. lard oil, some enzymes This group of linoleic acid, which cannot be produced by a human body due to its deficiency (LA) and alpha-linolenic acid (ALA). Other polyunsaturated fat acids, namely y-linolenic acid, oleic acid and palmitoleic acids, metabolic changes It can be produced by the body as the relevant enzyme is present in the body [6], [15].

Linoleic acid, like other acids in this group, is the most important among all omega-6 acids. 25418.101 It is considered to be important, plays a role in the synthesis of ALA or GLA7 [6], [10], complex lipids Y. lard oil also contains complex lipids including: moisturizing and softening the skin, softening the epidermis, increases its elasticity, reduces inflammation of the skin, promotes skin regeneration and cell phospholipids and glycolipids that accelerate regeneration. For example, the phospholipid group lecithin (also known as phosphatidylcholine), skin regenerating and It has moisturizing properties, slows down the aging process and also removes excess hair from the hair. removes oil (sebum). According to Shugam et al., the content of Y. igdem oil the total phospholipid content is 1% by weight. The lecithin content in this oil is thin layer determined by chromatography [57]. Other scientific studies [58], Y. igde Pericarpine oil contains 0.2-0.5% to 1% phospholipid by weight. confirmed. Of these, 5.8% by weight is lecithin. skin's lipid barrier sterols that strengthen it, protect it from harmful substances originating from the teeth and pass through the epidermis. reduces excessive water loss, thereby increasing skin elasticity and firmness [59], [60]. y. The presence of small amounts (less than 1% by weight) of liposomes in lard proven, the active ingredients as well as skin tightening and skin It allowed it to bind with ceramides that smooth it [61].

The importance of fatty acids in lard oil for the skin Linoleic acid found in larch oil plays an important role in the skin. on dry skin It strengthens the lipid barrier of the epidermis and protects against transepidermal water loss.

In addition, Linoliic acid (LA) regulates skin metabolism [6], [17], [18], [52], It has been noted that there is a decrease in the content of linoleic acid in sebum in people with diabetes mellitus.

As a result, blackheads and spots occur. In the care of oily and problematic skin The linoleic acid used can stimulate the functions of the sebaceous glands, It can unblock it and limit the number of blackheads. LA, intercellular It is also used in the production of the substance [6], [53], [56]. Y. gamma- 25418.101 Linolenic acid (GLA) is synthesized from linoleic acid by the enzyme delta-6-desaturase. Alpha- GLA, together with linolenic acid (ALA), is an important component of the cell membranes of human cells or It is a component of mitochondrial membranes [6], [10], [52], [56]. GLA and ALA also It is also responsible for normal intra-cerebral and intercellular transport (forming the brain). including the transmission of stimuli in the neuronal network) [6], [10], [54], [63]. unsaturated fat acids, especially in the omega-3 group (mainly EPA and DHA), neoplastic development of tumors, as well as neoplastic tissue and later metastasis It is assumed to inhibit its development [64]. At the same time, these acids are a harmful It can also reduce post-inflammatory substances caused by UV radiation. has been proven. These compounds reduce the effects of sunburns, accelerates the regenerative processes of the damaged lipid barrier and reduces irritation [48], [49], omega-3 (alpha-linolenic acids) reduce transepidermal water loss and increases hydration level [53], [55], [56]. Unsaturated FA, skin and proteins stimulates a portion of receptors that stimulate the synthesis of barrier lipids [54], [55], Y. igde oil in cosmetic products Y. lard oil is used in the cosmetic industry, especially in formulas made for mature skin. used [15], [54]. In anti-aging and anti-wrinkle products, the most it also reduces wrinkles in sagging skin and firmed tone [54]. Y. lard oil dry, irritated (for example, after sunbathing), rough, flaking and itchy it is also suitable for skin care [40], [52]. Treatment of frostbite and skin damage It is used as an auxiliary product in cases caused by exposure. For example It is used as a soothing agent after cosmetic procedures such as hair removal.

In shampoos, conditioners or after dead or permanent wave treatment its presence in the preparations used supports the regeneration of damaged hair, restores its elasticity and provides smoothness. High unsaturated fatty acids 25418.101 content [45], [52] and accordingly Y. lard oil in the form of capsules in cosmetics. It is recommended to use [46], [65].

European patent application no. EP3413979 from applications known in the art oil in a mixture of droplets of different sizes. oil-in-water emulsions is mentioned. The emulsions described herein are intended for cosmetic use. is expressed. In the invention, preferably wild igde (Seabuckthorn, Components selected from a group including Hippophae Rhamnoides) oil is stated to be possible. In addition, there may be unsaturated fatty acids and It is stated that it may be from a group that also includes linoleic acid and y-linolenic acid. is being done. In this invention, it is not of homogeneous size; drops of different sizes has been obtained. That is, based on the separation of two liquid phases into a concentrated colloidal phase, a chemical method for producing polymer droplets in suspension developed. topical personal care containing plant extracts formulations are mentioned. Plants that can be used within the scope of the invention Among them, wild igloo (Hippophae Rhamnoides) is also included. plant extracts encapsulates for delivery of additional components to a target area such as the skin. can be mentioned. Among the components that can be used here It also contains linoleic acid and linolenic acid.

French patent application no. FR2943255 from applications known in the art In the document, the lipophilic extract of wild igdenine (Hi'ppophae Rhamnoides), skin disorders caused by hormonal imbalances related to menopause It is mentioned that it is used in a composition produced for the treatment of Meet In this context, the extract is obtained by supercritical CO2 extraction technology. is expressed. 25418.101 Chinese patent application no. CN107137319 from applications known in the art from a nano-soft-delicate detoxifying cream is mentioned. This cream contains wild igloo (Hippophae Rhamnoides). received is indicated. The production method of the cream, which is the subject of the invention, is the cosmetic product. It consists of two basic steps, the first of which is the (Hzppophae Rhamnoides) extract, mixing the components and The second step is to produce this mixture in liposomes using liposome technology. is encapsulation.

In the Turkish patent application numbered TR 2017/15945, one of the applications known in the art, A method of producing a rash prevention and treatment cream is mentioned. This invention Thanks to its production and the formula it contains, without causing any side effects, by rejuvenating the skin and renewing the cells, both in the prevention and manufacture of a rash prevention and treatment cream that can be used to treat relates to method.

Brief Description of the Invention The aim of the invention is to obtain by encapsulation method using liposomal technology. Due to the regenerative effect of Wild Igde (Hippophae Rhamnofdes) extract It is used in personal care product formulations.

Detailed Description of the Invention To achieve the purpose of this invention, the “Encapsulated Wild A Personal Care Product Containing Igde (Hippophae Rhamnoides) Extract Its Formulation and Its Production Method” are shown in the attached figures; This from the figures: Sekill- Obtained after encapsulation of wild oil extract The particle size (size) in the mixture depends on the density. graphical representation of the change. 25418.101 Skin cell of the formulation prepared from wild berry extract It is the percentile vitality graph on which it is shown depending on the dose. is the display. It is a schematic representation of the production stages.

Obtaining unilamellar vesicle by encapsulation method sematically explained.

The parts in the figures are numbered and their equivalents are given below. UVWEAcs.

High Pressure Input - Pi (High Pressui'e [niet-P 1) Low Pressure Outlet-Pz (Low Pressure Outlet-Pz) High Shear Zone (Hiû= h Shear Zone) High Energy Impact Zone Multilamellar liposomes (pre-emulsion) + Actives (Mu1tilamellar) liposomes + Actives) unilamellar vesicles containing encapsulated actives (Unilamellar vesicles With encapsulated actives) Microfluidizer Processing Fat Phase Water Chard Centellin Phase Active Phase Emulsion Wild Igde Phase with Liposome Invention, Wild igdem (Hippophae rhamnoides) obtained by encapsulation method Regenerative personal care product formulations prepared with extract relates to development. Within the scope of the invention, a-tocopherol with lipophilic properties and b-carotene, in order to benefit from molecules with antioxidant structure, Y. igde 25418.101 oil in emulsion (E) form by encapsulating with liposomal technology. its use in formulations is discussed.

Liposomes have become the focus of attention in many medical applications in recent years. has arrived. such as the fluidity, permeability and molecular organization of liposomes. Physicochemical studies focused on the properties of natural It reveals the importance of lipid bilayers as the structural element of membranes. puts it. Liposome-cell interactions, fusion and adhesion in cell biology It provides a model for physiological events such as In addition, cell-cell improve the fusion of membranes, change the phospholipid and cholesterol content of the membranes and molecules that are normally water-soluble but difficult to pass into the cell. can be used for transfer. Liposomes are composed of layered phospholipids. They are very small (nanometer-sized) structures containing aqueous phase.

Liposomes will basically increase the efficacy of the active substances and remove unwanted in the transport of substances very selectively, reducing their toxic properties is used. In other cases, liposomes can prolong the effect, in improving adsorption or simply stabilizing active substances is used. Their size and physicochemical properties indicate that these structures are encapsulated. releasing the product into the tissue, causing its penetration and diffusion into the tissue in different ways. opens. Liposome-cell interactions, fusion and adhesion in cell biology It provides a model for physiological events such as In addition, cell-cell improve the fusion of membranes, change the phospholipid and cholesterol content of the membranes and molecules that are normally water-soluble but difficult to pass into the cell. can be used for transfer.

Phospholipids are the building blocks of liposomes and cell membranes. Lipids are usually They are hydrophobic and nonpolar. Therefore, they do not mix with water. But, Since the phosphate group of phospholipids has a hydrophilic and polar structure, they can be mixed. The structure of the liposome is basically similar to the cell membrane structure. shows. Liposomes contain both hydrophilic polar and hydrophobic apolar spaces. contains. In this way, water-soluble substances in polar spaces, oil 25418.101 by encapsulating soluble substances in their hydrophobic spaces, they can act as a carrier.

The cosmetic product formulations created within the scope of the invention Thanks to its active content and formulation prepared with superior technology, the cell provides renewal. Wild igdem extract, which is a cosmetic active ingredient, It is prepared by encapsulation using liposomal technology. Wild In addition to igden (Hippophae rhamnoi'des), the formula developed within the scope of the invention found; Hyaluronic acid, Helianthus Annuus (Sunilower) Seed Oil, Centella Thanks to its natural ingredients of Asiatica Extract, Glycine Soja (Soybean) Protein While increasing the regeneration of cells, Tocopherol Acetate and Superoxide Dismutase It provides protection from oxygen radicals that damage the skin.

Vegetable oils are generally derived from various parts of plants such as seeds and fruits. obtained by mechanical extraction or cold pressing. Chemical These oils contain ester-linked glycerol compounds and long aliphatic chain hydrocarbons. (min. Cl4: 0) are higher fatty acids. Vegetable oils, depending on their saturation percentage has various features. Within the scope of the invention, well-characterized Olive oil obtained from igneous fruits and seeds is used. Wild The igneous plant is used in cosmetic products due to its many active ingredients. It is used successfully in the industry and in the field of medicine. found in Y. igde oil valuable ingredients play an important role in the proper functioning of the human body and It gives a healthy appearance, Balanced amount of fatty acids it contains, types, amounts of vitamins, in dry or fast-aging cosmetic products explains its use for maintenance. Palmito-oleic acid (omega-7) and y-linolenic unsaturated fatty acids, such as acid (omega-6), help regenerate and repair the skin. it provides. Y. lard oil accelerates blood circulation, facilitates oxygenation of the skin, It removes excess toxins from the body and easily penetrates through the epidermis. Most Due to its feature, olive oil is a valuable product used in health and beauty products. it is a contribution. 25418.101 The subject of the invention is the personal care product used for anti-aging in the cosmetic industry. production method of the formulation of the product; Centellin is added to the water phase (SF) after dissolving at 40 oC, Emulsion (E) at 80 0C by adding the oil phase (YF) to the water phase (SF) (Oil-in-Water) creation, Mixing and cooling of the emulsion (E), After the emulsion (E) is cooled to 60 0C, the homogenizer homogenizing and reducing the mixing speed, When the emulsion (E) temperature reaches 30 0C, it is in the active phase (AF) adding the found ingredients to the emulsion (E), Re-homogenizing the emulsion (E) and closing, The addition of the liposome Wild Ig oil phase to the emulsion (E) and Continuing mixing until homogenized mixture is obtained, Adjusting the emulsion (E) pH to be 5.5 - 6.5, The emulsion (E) used as a personal care formulation is obtained. includes steps.

Creating Phases 1. Formation of Oil Phase (YF) The ingredients in the oil phase (YF); by weight, 0.001-0.01% Tocopherol; 0.1% - Lecithin; 0.2 - 0.7% Palmitic acid; 1 - 5% Caprylic/capric triglycerides, respectively weighed and poured into a glass beaker and heated to 80 °C. 2. Formation of the Water Phase (SF) Ingredients in the water phase (SF); by weight, 0.3 - 0.7% Caffeine; 0.05 - 0.3% - 1.0 Hyaluronic acid; 0,1 - 1,0% Allantoin, respectively, in a glass beaker.

Adjusting the created mixture to pH:5,5 and heating it to 80 0C 25418.101 3. Creation of Centellin Phase (CF) Extract of Centellin (Centeli'a asiatica) plant 0.2% - 0.7% propylene by weight Dissolution in glieol in a glass beaker at 40 0C 4. Establishment of Active Phase (AF) Ingredients in active phase (AF), by weight, 0.4% 2 1.5 Sorbitan caprylate; 0.01% dismutase; 0.4 - 1.5% Tocopherol Acetate . Formation of Liposome Wild Igland Phase (LYIF) Liposome preparation process: To prepare 100 g Liposome Solution; 2 grams of Lecithin is weighed and 88 by mixing at 20,000 rpm using a homogenizer in grams of water. dissolution is provided. 10 grams of this obtained aqueous Liposome solution By adding Wild Igde Oil and using a homogenizer for another 5-10 minutes, 20.000 It is mixed at rpm. The resulting mixture is 20.000 psiide microfludizer. It is made homogeneous by passing it 3 times (passes).

Wild Igde with Liposome (seabuekthorn- SB) % content by weight solution LECITHIN 2 Wild mulberry oil used in the scope of the invention was obtained by supercritical extraction method. is obtained and does not contain inorganic salts. Microfluidics In the (Microtludizer) system, 0.6% lecithin (lecithin) in the wild igda extract encapsulated with solution. The encapsulation process of this extract homogeneous molecules before and after encapsulation. 25418.101 It is being checked whether or not. Out of the chamber 3 times with the microfluidizer device The homogeneity distribution of the extracted extract was observed (Figure 1).

Accordingly, 931% of the molecules in the extract containing encapsulated Y. igde It is 314 nm in size. In this study, homogeneous Y. igde molecules were obtained. is seen.

Within the scope of the invention, for encapsulation of Y. lard oil with phospholipid. Microfluidizer processor (MP) (microfluidizer) system is used.

The working principle of Microtludizer liquid systems, liquid emulsifying systems or strong collision of solid matter dispersed in the liquid with each other is based on. This process is performed in the interaction Chamber with high energy density. it occurs. MicroIluidizer: Particle size reduction, Cell fragmentation, Emulsions (E), Reaction technology (aka Microtluidics Reaction It is used in Technology (MRT)) operations. The whole product, as one type, obtained continuously and under the same processing conditions and can be reproduced with such results It works in one way: Small particle or droplet size (usually microns) six), Uniform particle or droplet size (small poly dispersion index), Completely scalable process and Transaction continuity. A “Z-type Chamber” The schematic representation of the microfluidizer is shown in Figure 3.

Microtluidizer processor (MP), which forces the material to pass through a very small hole. acts as a great pump. Operating pressures start around 3.4 MPa/ 500 psi and up to 275 MPa / 40,000 psi. Process pressure, desired particle size varies according to different applications in order to obtain the distribution of Microfluidizer The processor (MP) emulsifies the liquid-liquid system together or a solid the said shear pressure, the effect of the pressure and the depends on the cavitation forces. This process is high in the interaction chamber. occurs at energy levels.

In the formulation, the extract in question is in the active phase (AF) as an active cosmetic ingredient. is used. Emulsion (E) created by using this extract 25418.101 the percentage of viability in which the formula is shown on the skin cell in a dose-dependent manner. (vitality) test was analyzed. vitality test; Promise according to ISO EN 10993-5 For the cream in question, ATCC-CCL-81 VERO cells were either pure (100%) or cellular A cytotoxicity study was performed on serial dilutions of culture medium. used. 10% Fetal Bovine Serum as medium (Gibco Cat no #10500-64) + Antibiotic-Antimycotic (Gibco #15240-062) with added MEM dilutions applied to cell culture. It was incubated for 24 hours at 37 °C and 5% CO2.

MTS/PMS solution was applied to the cells. Formazan formed at the end of incubation The crystals were measured in a spectrophotometer at a wavelength of 490nm-630nm.

Cytotoxicity indexes were calculated according to the positive and negatives, and the cytotoxicity of the cream was calculated. effect was tested. In conclusion; toxic effect on cells in cell culture does not show. In addition, 0.05% concentration of the tested cream It was observed that it increased its proliferation by 136%.

Within the scope of the invention, liposomed wild buckthorn oil showing a homogeneous distribution drops; 93% of it was obtained homogeneously at 314 nm size. In this way you get emulsion (E) formula increased cell renewal by 136%. has been found.

During the development of the invention, the microbiological analysis of the obtained cosmetic product Staphylococcus at 25 °C and 40 °C according to the microbiological compatibility test studied aureus, Pseudomonas aeruginosa, Candida albicans or Escherichia coli reproduction was not detected.

Claims (1)

REQUESTS 1. It is a method of obtaining the extract of wild buckthorn (Hippophae Rhamnoides) encapsulated with liposomal technology as a personal care product used for anti-aging purposes in the cosmetics industry; Centellin is dissolved at 40 °C and then added to the water phase (SF), the oil phase (YF) is added to the water phase (SF), forming an emulsion (E) (Oil-in-water) at 80 0C, mixing the emulsion (E) and Cooling the Emulsion (E) to 60 CC temperature, then homogenizing it with a homogenizer and reducing the mixing speed, Adding the ingredients in the active phase (AF) to the emulsion (E) when the temperature of the Einulsion (E) reaches 30 0C, Homogenizing again and turning off the homogenizer, Adding the Wild Irland oil phase with Liposome to the einulsion (E) and continuing to mix until the homogenized mixture is achieved, Adjusting the pH of the emulsion (E) to 5.5 - 6.5, Used as a personal care forinulation includes the steps of obtaining the emulsion (E). Lecithin; 0.2% 7 0.7 Palmitic acid; The method of obtaining the extract of wild egret (Hippophae Rhamnoides) encapsulated by liposomal technology as a personal care product in claim 1, characterized by containing 1 75% Caprylic/capric triglycerides. 3. The method of obtaining the extract of wild licorice (Hippophae Rhamnoides) encapsulated by liposomal technology as a personal care product in Istem Zadeki, which is characterized by weighing the ingredients in the oil phase (YF) and placing them in a glass beaker and heating them to a temperature of 80 0C. The method of obtaining the extract of wild licorice (Hippophae Rhamnoides) encapsulated by liposomal technology as a personal care product in Claim 1, which is claimed. . As a personal care product in Claim 4, which is characterized by taking the contents of the water phase (SF) into a glass beaker and adjusting the resulting mixture to pH:5.5 and heating it to a temperature of 80 0C, wild buckthorn (Hippophae Rhamnoides) extract encapsulated by liposomal technology is obtained. method of making. . Encapsulated by liposomal technology as a personal care product in Claim 1, characterized by the dissolution of Centellin (Centella Asiatica) extract in 0.2 - 0.7% propylene glycol by weight in a glass beaker at 40 CC to form the centellin phase (CF). Method of obtaining wild igde (Hippophae Rhamnoides) extract. dismutase; The method of obtaining the extract of wild buckthorn (Hippophae Rhamnoides) encapsulated by liposomal technology as a personal care product in claim 17, characterized by containing 0.4% 7 1.5 Tocopherol Acetate. . For the formation of Liposome Wild Igda Fazi (LYIF), by weight, 2% Lecithin and 88% water are mixed at 20,000 rpm using a homogenizer and 10 grams of Wild Igland Oil is added to the obtained aqueous Liposome solution and heated at 20,000 rpm for another 5-10 minutes. The method of obtaining the extract of wild licorice (Hippophae Rhamnoides) encapsulated by liposomal technology as an optional personal care product, characterized by mixing. 9. Wild Igland (Hippophae Rhamnoides) encapsulated by liposomal technology as a personal care product in Claim 1, which is characterized by the fact that 93% of the molecules in the extract containing Y. igde encapsulated in Liposome Wild Igland Phase (LYIF) are 314 nm in size. Extraction method.