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TR200003759T2 - Genes for the biosynthesis of epothilons - Google Patents

Genes for the biosynthesis of epothilons

Info

Publication number
TR200003759T2
TR200003759T2 TR2000/03759T TR200003759T TR200003759T2 TR 200003759 T2 TR200003759 T2 TR 200003759T2 TR 2000/03759 T TR2000/03759 T TR 2000/03759T TR 200003759 T TR200003759 T TR 200003759T TR 200003759 T2 TR200003759 T2 TR 200003759T2
Authority
TR
Turkey
Prior art keywords
epothilone
biosynthesis
genes
epothilons
encodes
Prior art date
Application number
TR2000/03759T
Other languages
Turkish (tr)
Inventor
Schupp Thomas
Madison Login James
Molnar Istvan
Zirkle Ross
G�Rlach J�Rn
Cyr Devon
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of TR200003759T2 publication Critical patent/TR200003759T2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Epotilonun biyosentezi için gerekli olan polipeptidleri kodlayan Sorangium cellulosum'dan nükleik asit molekülleri izole edilmistir. Epotilonun, bu bulusun genleri ile dönüstürülmüs rekombinant ev sahibi hücrelerde üretilmesi için olan metotlar burada açiklanmaktadir. Bu sayede epotilon, kanser tedavisi için olan farmasötik formülasyonlarda saflastirilmasini ve kullanimi saglayacak bir biçimde yeterince büyük bir miktarda üretilmektedir.Nucleic acid molecules were isolated from Sorangium cellulosum, which encodes the polypeptides necessary for the biosynthesis of the epothilone. Methods for producing epothilone in recombinant host cells transformed with the genes of this invention are described herein. In this way, epothilone is produced in a sufficiently large amount to provide its purification and use in pharmaceutical formulations for cancer treatment.

TR2000/03759T 1998-06-18 1999-06-16 Genes for the biosynthesis of epothilons TR200003759T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9950498A 1998-06-18 1998-06-18
US10163198P 1998-09-24 1998-09-24
US11890699P 1999-02-05 1999-02-05

Publications (1)

Publication Number Publication Date
TR200003759T2 true TR200003759T2 (en) 2001-06-21

Family

ID=27378840

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2000/03759T TR200003759T2 (en) 1998-06-18 1999-06-16 Genes for the biosynthesis of epothilons

Country Status (16)

Country Link
EP (1) EP1088078A2 (en)
JP (3) JP2002518004A (en)
KR (1) KR100511233B1 (en)
CN (1) CN100374565C (en)
AU (1) AU753567B2 (en)
BR (1) BR9911349A (en)
CA (1) CA2329774A1 (en)
HU (1) HUP0102186A3 (en)
ID (1) ID29128A (en)
IL (3) IL139735A0 (en)
NO (2) NO20006195L (en)
NZ (1) NZ508326A (en)
PL (1) PL200157B1 (en)
SK (1) SK19242000A3 (en)
TR (1) TR200003759T2 (en)
WO (1) WO1999066028A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204388B1 (en) 1996-12-03 2001-03-20 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto and analogues thereof
CA2273083C (en) 1996-12-03 2012-09-18 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto, analogues and uses thereof
FR2775187B1 (en) 1998-02-25 2003-02-21 Novartis Ag USE OF EPOTHILONE B FOR THE MANUFACTURE OF AN ANTIPROLIFERATIVE PHARMACEUTICAL PREPARATION AND A COMPOSITION COMPRISING EPOTHILONE B AS AN IN VIVO ANTIPROLIFERATIVE AGENT
DE19846493A1 (en) * 1998-10-09 2000-04-13 Biotechnolog Forschung Gmbh DNA sequence coding for products involved in the biosynthesis of polyketide or heteropolyketide compounds, especially epothilone
US6410301B1 (en) 1998-11-20 2002-06-25 Kosan Biosciences, Inc. Myxococcus host cells for the production of epothilones
NZ511722A (en) 1998-11-20 2004-05-28 Kosan Biosciences Inc Recombinant methods and materials for producing epothilone and epothilone derivatives
WO2001053533A2 (en) * 2000-01-21 2001-07-26 Kosan Biosciences, Inc. Method for cloning polyketide synthase genes
NZ546497A (en) * 2000-04-28 2008-04-30 Kosan Biosciences Inc Production of polyketides
US6998256B2 (en) 2000-04-28 2006-02-14 Kosan Biosciences, Inc. Methods of obtaining epothilone D using crystallization and /or by the culture of cells in the presence of methyl oleate
US7257562B2 (en) 2000-10-13 2007-08-14 Thallion Pharmaceuticals Inc. High throughput method for discovery of gene clusters
IL155306A0 (en) 2000-10-13 2003-11-23 Univ Mississippi Methods for producing epothilone derivatives and analogs and epothilone derivatives and analogs produced thereby
DK1483251T3 (en) 2002-03-12 2010-04-12 Bristol Myers Squibb Co C3-cyano-epothilone derivatives
WO2006083721A2 (en) * 2005-01-31 2006-08-10 Merck & Co., Inc. Purification process for piasmid dna
WO2012103516A1 (en) 2011-01-28 2012-08-02 Amyris, Inc. Gel-encapsulated microcolony screening
US20140057314A1 (en) 2011-05-13 2014-02-27 Amyris, Inc. Methods and compositions for detecting microbial production of water-immiscible compounds
WO2014025941A1 (en) 2012-08-07 2014-02-13 Jiang Hanxiao Methods for stabilizing production of acetyl-coenzyme a derived compounds
EP2971027B1 (en) 2013-03-15 2019-01-30 Amyris, Inc. Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds
PT3030662T (en) 2013-08-07 2020-03-25 Amyris Inc Methods for stabilizing production of acetyl-coenzyme a derived compounds
US10808015B2 (en) 2015-06-25 2020-10-20 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds
CN106916834B (en) * 2015-12-24 2022-08-05 武汉合生科技有限公司 Biosynthetic gene cluster of compounds and application thereof
CN111138444B (en) * 2020-01-08 2022-05-03 山东大学 Epothilone B glucoside compounds and enzymatic preparation and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0941227T5 (en) * 1996-11-18 2009-10-05 Biotechnolog Forschung Gmbh Epothilone D, its preparation and its use as a cytostatic agent and as a plant protection agent

Also Published As

Publication number Publication date
NZ508326A (en) 2003-10-31
HUP0102186A2 (en) 2001-10-28
WO1999066028A3 (en) 2000-06-29
IL190391A0 (en) 2008-11-03
NO20006195D0 (en) 2000-12-06
PL200157B1 (en) 2008-12-31
AU4611699A (en) 2000-01-05
BR9911349A (en) 2001-03-13
CN1305530A (en) 2001-07-25
IL139735A0 (en) 2002-02-10
AU753567B2 (en) 2002-10-24
SK19242000A3 (en) 2001-07-10
NO20006195L (en) 2001-02-16
PL345579A1 (en) 2001-12-17
KR100511233B1 (en) 2005-08-31
HUP0102186A3 (en) 2005-10-28
JP2008092958A (en) 2008-04-24
JP2002518004A (en) 2002-06-25
IL139735A (en) 2009-06-15
JP2006061166A (en) 2006-03-09
NO20091055L (en) 2001-02-16
ID29128A (en) 2001-08-02
KR20010052962A (en) 2001-06-25
CN100374565C (en) 2008-03-12
WO1999066028A2 (en) 1999-12-23
EP1088078A2 (en) 2001-04-04
CA2329774A1 (en) 1999-12-23

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