SU908357A1 - Method of producing collagenic material - Google Patents

Description

The invention of otnostis to the processing of collagen-containing raw materials and may find application in veterinary surgery in the preparation of collagen material with hemostatic properties.

A known method for producing collagen material includes alkali salt treatment of a collagen containing material with a saturated solution of sodium sulfate, containing caustic sodium, salt washing with a saturated solution of sodium sulfate, neutralization with an inorganic acid, swelling of the collagen mass in 1 M acetic acid and freeze-drying 1.

Collagen material obtained by this method is used as food packaging, filters in the manufacture of tobacco products, as well as used in the treatment of burns and ulcers on the body. Collegene sponges in this method are finely porous, the pore walls have insufficient mechanical strength and resistance to the action of tissue fluid and blood and do not provide the necessary hemostatic effect, due to the fact that pretreatment of the derma with 5-15% sodium hydroxide destroys a large amount alkaline-stable bonds in the structure of collagen. .

The purpose of the invention is to obtain a highly porous collagen material having hemostatic properties.

This goal is achieved by

10 that the alkali-salt treatment of the nso-containing raw material collage is carried out in a saturated sodium sulfate solution containing 3-4% sodium hydroxide, after the collagen mass swells

15 in 1 M acetic acid, mechanical dispersion of the collagen mass is carried out in a homogenizer, 6 during which 2-10% is introduced into the dispersible lagengen mass

epsilonaminocaproic acid from the absolutely dry residue of the collagen dispersion.

Example. Take 1 kg of Achilles tendons of cattle,

Claims (1)

25 is cut into 0.5 x 0.5 cm pieces and pierced with running water for 1 hour. To remove non-collagen proteins, fat, and also to prepare the raw material for homogeneous state, the raw material is processed. an alkali-salt mixture of the following composition: J 1.636 liters of water, 284 g of sodium sulfate (anhydrous) and 80 g of sodium hydroxide, for 72 hours at 18–20 ° C. To remove the alkali from the worked material with the indicated solution, the salt was washed with 1 M sodium sulfate solution for 5 hours and neutralized with 3% diluent HjBOg. After alkali is removed from the collagen containing raw material, neutral salts (sulfates and sodium chlorides) are removed by thorough washing in running water until complete removal of the sulfate ions, as determined by OTcyTCTBtzra, by washing the cloudy water under the action of barium chloride. The resulting collagen mass is filled with a threefold volume of 1 M aqueous acetic acid solution to swell the collagen and significantly separate the fibrillar structures. After swelling of the collagen mass, after 4V hours, it is mechanically dispersed in a tissue remover (homogenizer) until a viscous, homogeneous mass is obtained at a rate of 5000b / lin for 5 minutes. As a result, a collagen dispersion with a concentration of 2.0-2.5% of absolutely dry residue with a pH of 3.0-3.2 is obtained. In the dispersion process, epsilon-aminocaproic acid is added to the collagen mass in an amount of 5 n formalin 2% of the absolute dry residue of the dispersions Collagen The resulting finished product is poured into cuvettes with a layer thickness of 10 mm, frozen at -40 ° C / and sublimated for 20 hours at a pressure of 0.10.2 mm Hg-0 column and a heat transfer temperature of 60 ° C. The ready collagen hemostatic material has the following physicochemical properties: 10 mm of Toldan plates 94-96% Porosity Cauchy density 0, 15-0,22 g / s weight True weight 1, 30-1.33 g / s weight 1,7 -2.8% Ash content 14-17%. Humidity 80-84.3%. Protein content The resulting material is sealed in plastic bags and sterilized with gamma radiation at a dose of 2 Mrad. The application of the proposed method in the bloody removal of the rudiments of horns from the t body reduces the time spent on the operation, provides reliable hemostasis, does not require additional therapeutic interventions in the postoperative period. The proposed method makes it possible to obtain a hemostatic material for its therapeutic properties that meet the requirements of surgery, for hemostatic materials. The hemistatic sponge obtained by this method is significantly reducing blood loss, as well as the time required for surgical intervention and improves the therapeutic effect of the treatment. The method of obtaining a collagen material, including alkali-salt treatment of a collagen-containing raw material with a saturated solution of sodium sulfate, containing caustic sodium, salt washing with a saturated solution of sodium sulfate, neutralization with an inorganic acid, swelling of the collagen mass in 1 M acetic acid and sublimation drying. in order to obtain highly porous collagen material with hemostatic properties, alkaline-salt treatment collagen encoder The total raw material is carried out in a saturated solution of sodium sulfate, containing 3-4% of iatry, after swelling of the collagen mass in 1 M acetic acid, mechanical dispersion of the collagen mass is carried out in a homogenizer, during which 2 are added to the dispersible collagen mass. -10% epsilonaminocaproic acid from the absolutely dry residue of the collagen dispersion. Sources of information taken into account in the examination 1, USSR Author's Certificate No. 456438, cl. A 23 J 1/10, 1974.