SU67718A1 - Method for detecting bacterial accumulations of hydrocarbon microflora in soil samples during bacterial prospecting for oil and combustible gases - Google Patents
Method for detecting bacterial accumulations of hydrocarbon microflora in soil samples during bacterial prospecting for oil and combustible gasesInfo
- Publication number
- SU67718A1 SU67718A1 SU292A SU334257A SU67718A1 SU 67718 A1 SU67718 A1 SU 67718A1 SU 292 A SU292 A SU 292A SU 334257 A SU334257 A SU 334257A SU 67718 A1 SU67718 A1 SU 67718A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- bacterial
- hydrocarbon
- microflora
- oil
- soil samples
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
При бактериальной разведке иа нефть и горючие газы необходимо непосредетвенно в поле ироизводить быстрые анализы дл обнаружени наибольшего сконлени углеводородных бактерий в грунте.With bacterial prospecting, oil and flammable gases must be directly in the field and rapid analyzes are performed to detect the greatest concentration of hydrocarbon bacteria in the soil.
Предлагаетс способ обнаружени бактериальных сконлений углеводородной микрофлоры в образцах грунтов при бактериальной разведке иа иефть и горючие газы, основанный на фиксации хемилюминесценции бактериальных скоплений, возннкающей носле обработки образцов грунта соответствующими реактивами.A method is proposed for detecting bacterial clutches of hydrocarbon microflora in soil samples during bacterial exploration and fuel and combustible gases, based on fixing the chemiluminescence of bacterial accumulations arising from the processing of soil samples with appropriate reagents.
Выиолиеиие предлагаемого снособа заключаетс в следующем. Образен грунта, отобранный из скважины, носле двухдневной обработки его минеральной средой Менца залнвают щелочным раствором люминола с перекисью водорода. Указанный раствор, нонада иа бактернальные скоплени , вызывает их -свеченне, хорошо заметное в темноте.The proposed solution is as follows. The soil is taken from a well, and during the two-day treatment with the mineral medium Mentz is ground with an alkaline solution of luminol with hydrogen peroxide. This solution, nonada and bacterial accumulations, causes them to be bright, clearly visible in the dark.
Дл фиксации этого эффекта нримеи етс фотоиластиика, которую неносредственно или через систему луи накладывают иоверх стаканчнков нри вь держке около 5-7 мин. По силе светлого п тна на пластинке суд т об интенсивности обнаруженного бактериальиого скоплени .To fix this effect, photoelasticity is applied, which, directly or through a Louis system, is applied over the top of the cup for about 5-7 minutes. The intensity of the detected bacterial cluster is judged by the strength of the bright spot on the plate.
Метод пригоден только дл обнаруженн значительных бактериальиы .х сконлений.The method is only suitable for detecting significant bacterial growth.
Предмет изобретени Subject invention
Способ обиаружени бактериальных сконлеинй углеводородной микрофлоры в образцах грунтов нри бактериальиой разведке на иефть н горючие газы, отличающийс тем, что иодлежащие исследоваиию образцы груитов после обработки их минеральной средой Менца заливают раствором люминола (гидразино-3-амииофталевой кислоты) с нерекисью водорода и фиксируют возникающую хемилюмииесценцию бактериальных скоплений.The method of bacterial growth of hydrocarbon microflora in soil samples using bacterial exploration for oil and combustible gases, characterized in that samples of grits after studying them with Mentz mineral medium are poured with a solution of luminol (hydrazine-3-amio-phthalic acid) with hydrogen. bacterial clusters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU292A SU67718A1 (en) | 1944-08-25 | 1944-08-25 | Method for detecting bacterial accumulations of hydrocarbon microflora in soil samples during bacterial prospecting for oil and combustible gases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU292A SU67718A1 (en) | 1944-08-25 | 1944-08-25 | Method for detecting bacterial accumulations of hydrocarbon microflora in soil samples during bacterial prospecting for oil and combustible gases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU67718A1 true SU67718A1 (en) | 1946-11-30 |
Family
ID=52016246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU292A SU67718A1 (en) | 1944-08-25 | 1944-08-25 | Method for detecting bacterial accumulations of hydrocarbon microflora in soil samples during bacterial prospecting for oil and combustible gases |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU67718A1 (en) |
-
1944
- 1944-08-25 SU SU292A patent/SU67718A1/en active
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