SU274488A1 - - Google Patents
Info
- Publication number
- SU274488A1 SU274488A1 SU1333948A SU1333948A SU274488A1 SU 274488 A1 SU274488 A1 SU 274488A1 SU 1333948 A SU1333948 A SU 1333948A SU 1333948 A SU1333948 A SU 1333948A SU 274488 A1 SU274488 A1 SU 274488A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- calcium
- flame
- photometer
- hydrochloric acid
- Prior art date
Links
- 239000000243 solution Substances 0.000 description 13
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 11
- 239000011575 calcium Substances 0.000 description 11
- 229910052791 calcium Inorganic materials 0.000 description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 2
- 229940010552 ammonium molybdate Drugs 0.000 description 2
- 235000018660 ammonium molybdate Nutrition 0.000 description 2
- 239000011609 ammonium molybdate Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004380 ashing Methods 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- -1 milk Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Description
Изобретение относитс к молочной промышленности , в частности к определению кальци в .молоке и .молочных продуктах фото.метрией пропан-воздушного пла.мени.The invention relates to the dairy industry, in particular to the determination of calcium in milk and milk products by photometry of a propane-air flame.
Известен способ определени содержани кальци в биологических объектах, содержаш ,их фосфаты, например в .молоке, предусматривающий озоление пробы исследуемого вещества и растворение золы, например, в сол ной кислоте с последуюш.им установлением количества кальци в растворе на пламенно.м фотометре. Однако, если в растворе присутствует анион фосфорной кислоты, то он сильно снижает чувствительность анализа, так как св зывает ионы кальци в прочное термостойкое соединение. Это не позвол ет определ ть кальций в присутствии аниона фосфорной кислоты , если концентраци кальци в растворе ииже 1 мг/мл, так как при это.м ркость излучени кальци в пла.мени сравнима с величиной фона Б приборе, а так как величина фона периодически .мен етс , ошибка определени может достигать значительной величины.A known method for determining the calcium content in biological objects containing phosphates, such as milk, provides for the ashing of a sample of the test substance and dissolving ash, for example, in hydrochloric acid, followed by determining the amount of calcium in the solution on a flame photometer. However, if a phosphoric acid anion is present in the solution, it greatly reduces the sensitivity of the assay, since it binds calcium ions into a strong, heat-resistant compound. This makes it impossible to determine calcium in the presence of a phosphoric acid anion if the calcium concentration in the solution is below 1 mg / ml, since at this time the radiation intensity of calcium in the plasma plate is comparable to the value of background B in the instrument, and since the background value periodically Changes, the error of determination can reach a significant value.
С целью определени кальци в пропанвоздушно .м пламени пламенного фотометра, а более точного определени содержани его и повышени чувствительности анализа, предлагаетс перед установлением количества кальци на пла.менно.м фотометре в раствор вносить молибдат аммони дл устранени вли ни фосфатов не менее 0,2 г на 1 мг фосфора , наход щегос в растворе в виде фосфатов .In order to determine the calcium in the propane-air flame of the flame photometer, and to more accurately determine its content and increase the sensitivity of the analysis, it is suggested to add ammonium molybdate to the solution before determining the amount of calcium in the flame meter. g per 1 mg of phosphorus, which is in solution in the form of phosphates.
Способ осушествл етс следующи.м образо .м.The method is implemented as follows.
Подлежащий исследованию образец озол ют одним из известных способов, золу раствор ют в сол ной кислоте и объе.м раствора довод т до исходного дистиллированной водой. Концентраци сол ной кислоты в полученном растворе 0,6 н.The sample to be examined is salted by one of the known methods, the ash is dissolved in hydrochloric acid, and the volume of the solution is brought to the original distilled water. The concentration of hydrochloric acid in the resulting solution is 0.6 n.
К 20 мл полученного раствора добавл ют 20 мл раствора : 1олибдата аммони (200 г/л) и 3 .ил ам.миачного буфера (22 е хлористого ам.мони и 80 мл концентрированного раствора амиака в 1 л буферного раствора). При это.м рН определ е.мого раствора устанавливают в пределах 5-6. Исследуемый раствор при помощи распылител ввод т в плам газовой горелки и сравнивают ркость пламени, получающуюс при этом с ркостью, получае.мой при введении стандартных растворов. Яркость пламени определ ют на длине волны 554 .U.U. В стандартные растворы ввод т буфер той же концентрации, что и в исследуемые .To 20 ml of the resulting solution are added 20 ml of a solution: ammonium lolybdate (200 g / l) and 3 ml of ammonia buffer (22 e of ammonium chloride and 80 ml of concentrated ammonia solution in 1 l of buffer solution). At that, the pH of the determined em of my solution is set within 5-6. The test solution is injected with a sprayer into the flame of a gas burner and compares the brightness of the flame, which is obtained with the brightness obtained with my introduction of standard solutions. The flame brightness is determined at a wavelength of 554 .U.U. Buffer with the same concentration was added to the standard solutions as to the studied ones.
Предмет изобретени Subject invention
--274488 --274488
3434
озоление пробы исследуемого вещества и ра-ределеии содержани его и повышени чувстстворение золы, например, в сол ной кислотевктельности анализа, перед установлением кос последующим установлением количествалпчества кальци на пламенном фотометре вthe ashing of a sample of the test substance and the dilution of its content and an increase in the ash sensation, for example, in hydrochloric acid in the analysis, before setting the streamer followed by determining the amount of calcium on the flame photometer in
кальци в растворе на пламенном фотометре,раствор внос т молибдат аммони дл устраотличающийс тем, что, с целью определени 5 нени вли ни фосфатов не менее 0,2 г на 1 мгcalcium in the solution on the flame photometer, the solution is applied ammonium molybdate for dissociating with the fact that, in order to determine 5% effect of phosphate at least 0.2 g per 1 mg
кальци в пропан-воздущном пламени пла-фосфора, наход щегос в растворе в виде фосменного фотометра, а также более точного оп-фатов.Calcium in the propane-air flame of phosphorus, which is in solution in the form of a phosphate photometer, as well as more accurate optic.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU274488A1 true SU274488A1 (en) |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3798000A (en) * | 1971-09-03 | 1974-03-19 | Merck Patent Gmbh | Agent and method for determination of calcium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3798000A (en) * | 1971-09-03 | 1974-03-19 | Merck Patent Gmbh | Agent and method for determination of calcium |
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