SU1623661A1 - Method for sterilization od demineralized bone matrix - Google Patents
Method for sterilization od demineralized bone matrix Download PDFInfo
- Publication number
- SU1623661A1 SU1623661A1 SU884611672A SU4611672A SU1623661A1 SU 1623661 A1 SU1623661 A1 SU 1623661A1 SU 884611672 A SU884611672 A SU 884611672A SU 4611672 A SU4611672 A SU 4611672A SU 1623661 A1 SU1623661 A1 SU 1623661A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- sterilization
- bone matrix
- demineralized bone
- treatment
- chlorhexidine
- Prior art date
Links
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 8
- 210000002805 bone matrix Anatomy 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title claims description 4
- 230000002138 osteoinductive effect Effects 0.000 claims abstract description 4
- 230000002421 anti-septic effect Effects 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 229960003260 chlorhexidine Drugs 0.000 claims description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 3
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 claims description 3
- 229910052740 iodine Inorganic materials 0.000 claims description 3
- 239000011630 iodine Substances 0.000 claims description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 2
- 210000000988 bone and bone Anatomy 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000000399 orthopedic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
Изобретение относитс к области медицины , а именно травматологии. Цель - сохранение остеоиндуктивных свойств. Стерилизацию деминерализованного костного матрикса осуществл ют путем обработки в антисептическом растворе при температуре 2+5°С и рН 6,8-7,0 в течение 1 сут.This invention relates to the field of medicine, namely, traumatology. The goal is to preserve the osteoinductive properties. Sterilization of the demineralized bone matrix is carried out by treatment in an antiseptic solution at a temperature of 2 + 5 ° C and a pH of 6.8-7.0 for 1 day.
Description
Изобретение относитс к медицине, а именно травматологии, ортопедии, и может быть использовано при массовой заготовке трансплантатов.The invention relates to medicine, namely to traumatology, orthopedics, and can be used for mass preparation of grafts.
Цель изобретени - сохранение остеоиндуктивных свойств.The purpose of the invention is the preservation of osteoinductive properties.
Способ осуществл ют следующим образом .The method is carried out as follows.
Деминерализованные костные трансплантаты помещают без соблюдени асептики в стерилизующий раствор, который приготавливают следующим образом.Demineralized bone grafts are placed without asepsis in a sterilizing solution, which is prepared as follows.
В 70° этиловый спирт на 100,0 мл добавл ют 0,2 мл спиртового 5%-ного раствора йода и 0,01 мл хлоргексидина. Дл стерилизации используют только свежеприготовленные растворы с рН 6.8-7,0, стерилизацию осуществл ют при температуре 2-5°С в течение 24 ч.In 70 ° ethyl alcohol per 100.0 ml, 0.2 ml of an alcoholic 5% iodine solution and 0.01 ml of chlorhexidine are added. For sterilization use only freshly prepared solutions with a pH of 6.8-7.0, sterilization is carried out at a temperature of 2-5 ° C for 24 hours.
Пример. Фрагменты бедренной аллокости размером 10,0-12,0x0,2-0,5 см очищают от м гких тканей и помещают в декаль- цинирующую жидкость 0,6 н. раствор сол ной кислоты при +4°С. Полученный костный матрикс промывают проточной RO- дой 4,0-4,5 ч до получени рН 7,2 - 7,4 После этого без соблюдени асептики костный матрикс помещают в стерилизующий раствор, который приготавливают следующим образом. В этиловый спирт 70е (100 мл) добавл ют 0,2 мл спиртового раствора иода и 0,01 мл хлоргексидина Дл стерилизации используют только свежеприготовленные растворы (рН 6,8-7,0), при температуре обработки 2-4°С. Врем стерилизации 24 ч. После стерилизации деминерализованный костный матрикс может быть использован в операции.Example. Fragments of the femoral allocracy, 10.0-12.0x0.2-0.5 cm in size, are cleaned from soft tissues and placed in a decal curing fluid of 0.6 n. hydrochloric acid solution at + 4 ° С. The resulting bone matrix is rinsed with flow-through pH 4.0-4.5 hours until a pH of 7.2-7.4 is obtained. After this, the bone matrix is placed in a sterilizing solution without asepsis, which is prepared as follows. In ethyl alcohol 70e (100 ml), 0.2 ml of an alcoholic solution of iodine and 0.01 ml of chlorhexidine are added. Only freshly prepared solutions (pH 6.8-7.0) are used for sterilization, at a treatment temperature of 2-4 ° C. The sterilization time is 24 hours. After sterilization, the demineralized bone matrix can be used in the operation.
Предложенный способ позвол ет сохранить остеоиндуктивные свойства трансплантата в 97% случаев, кроме того, достигнут объем индуцированной новообразованной кости, приближающийс к объему индуцированной новообразованной кости в стерильно заготовленном деминерализованном костном матриксе.The proposed method allows preserving the osteoinductive properties of the graft in 97% of cases, in addition, a volume of induced new bone was achieved, approaching the volume of induced new bone in a sterile prepared demineralized bone matrix.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884611672A SU1623661A1 (en) | 1988-10-17 | 1988-10-17 | Method for sterilization od demineralized bone matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU884611672A SU1623661A1 (en) | 1988-10-17 | 1988-10-17 | Method for sterilization od demineralized bone matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1623661A1 true SU1623661A1 (en) | 1991-01-30 |
Family
ID=21412155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU884611672A SU1623661A1 (en) | 1988-10-17 | 1988-10-17 | Method for sterilization od demineralized bone matrix |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1623661A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2156139C1 (en) * | 1999-03-15 | 2000-09-20 | Волова Лариса Теодоровна | Method for sterilizing lyophilized bone grafts |
| EP1708651A4 (en) * | 2004-01-27 | 2011-11-02 | Osteotech Inc | Stabilized bone graft |
-
1988
- 1988-10-17 SU SU884611672A patent/SU1623661A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Иванкин Д. И Биопластические свойства деминерализованных и недеминерализованных костных аллотрансплантатов, консервированных в жидких средах. - В кн.: Заготовка и пересадка деменсрализонанной костной ткани в эксперименте и клинике, 1983, с. 180. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2156139C1 (en) * | 1999-03-15 | 2000-09-20 | Волова Лариса Теодоровна | Method for sterilizing lyophilized bone grafts |
| EP1708651A4 (en) * | 2004-01-27 | 2011-11-02 | Osteotech Inc | Stabilized bone graft |
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