SU1698288A1 - Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum - Google Patents
Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum Download PDFInfo
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- SU1698288A1 SU1698288A1 SU904788265A SU4788265A SU1698288A1 SU 1698288 A1 SU1698288 A1 SU 1698288A1 SU 904788265 A SU904788265 A SU 904788265A SU 4788265 A SU4788265 A SU 4788265A SU 1698288 A1 SU1698288 A1 SU 1698288A1
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- strain
- influenza
- leningrad
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- 241000700605 Viruses Species 0.000 title claims abstract description 17
- 206010022000 influenza Diseases 0.000 title claims abstract description 5
- 230000000890 antigenic effect Effects 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims abstract description 5
- 208000037801 influenza A (H1N1) Diseases 0.000 claims abstract description 3
- 238000001228 spectrum Methods 0.000 claims abstract description 3
- 201000010740 swine influenza Diseases 0.000 claims abstract description 3
- 208000037797 influenza A Diseases 0.000 claims description 5
- 241000700159 Rattus Species 0.000 claims 2
- 230000000521 hyperimmunizing effect Effects 0.000 claims 2
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 101100309570 Caenorhabditis elegans let-765 gene Proteins 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000185 hemagglutinin Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Изобретение относитс к вирусологии. Целью изобретени вл етс штамм вируса гриппа A(H1N1), обладающий высокой диагностической активностью. Дл этого выдел ют штамм А/Ленинград/325/88(НШ1). Данный штамм характеризуетс широким антигенным спектром и высокой авидно- стью к антигенам. Fro применение позволило увеличить частоту вы вл емых сероконверсий, что в свою очередь повысило точность диагностики гриппозной инфекции . 3 табл. СО сThis invention relates to virology. The aim of the invention is an influenza A (H1N1) virus strain having high diagnostic activity. For this, strain A / Leningrad / 325/88 (NSh1) is isolated. This strain is characterized by a wide antigenic spectrum and high avidity for antigens. Fro application allowed to increase the frequency of detected seroconversions, which in turn increased the accuracy of diagnosis of influenza infection. 3 tab. SO with
Description
Изобретение относитс к вирусологии. Целью изобретени вл етс штамм вируса гриппа A(H1N1), обладающий высокой диагностической активностью.This invention relates to virology. The aim of the invention is an influenza A (H1N1) virus strain having high diagnostic activity.
Морфологические признаки.Morphological signs.
Полиморфна структура: типична дл вирусов гриппа.Polymorphic structure: typical of influenza viruses.
Физические свойства.Physical properties.
Коэффициент седиментации 750S, плавуча плотность 1,21 г/см3.Sedimentation coefficient 750S, buoy density 1.21 g / cm3.
Репродукционна активность.Reproductive activity.
Титр гемагглютининов - 1:128-1:256 в 0,2 мл с 1 % эритроцитами кур, инфекционный титр - 7,5 Ig ЭИДбО/0,2 мл.The titer of hemagglutinins - 1: 128-1: 256 in 0.2 ml with 1% chicken erythrocytes, infectious titer - 7.5 Ig EIDbO / 0.2 ml.
Стабильность биологических свойств штамма А/Ленинград/ 325/88(H1N1) доказана при 5-кратном пассировании.The stability of the biological properties of strain A / Leningrad / 325/88 (H1N1) was proved with 5-fold passaging.
Пример. Анализ использовани штамма показал следующее. По результатам перекрестной РТГА с крысиными моно- специфическими сыворотками штамм характеризовалс широким антигенным спектром по отношению к антителам к современным вирусам (табл.1).Example. An analysis of the use of the strain showed the following. According to the results of cross-rtga with rat monospecific sera, the strain was characterized by a broad antigenic spectrum with respect to antibodies to modern viruses (Table 1).
Кроме того, штамм вируса гриппа А/Ле- нинград/325/88 сохранил широкий спектр антигенной активности и авидность к антителам: Б (табл.2) перекрестной РТГА он реагируете антисывороткой к вирусу А/Прага/1 /84In addition, the strain of influenza A / Leningrad / 325/88 virus retained a wide range of antigenic activity and avidity for antibodies: B (Table 2) with cross RTGA, it reacts with antiserum to virus A / Prague / 1/84
OsOs
юYu
00 Ю 00 0000 Y 00 00
до 1/4 титра, с антисывороткой к вирусу А/Тэйвань/1/86 -до полного титра и с антисывороткой к вирусу А/Мурманск/890/88 - до титра, в два раза превышающего титр этого штамма с гомологичной антисывороткой ,up to 1/4 of the titer, with antiserum to the virus A / Teivan / 1/86 - up to full titer and with antiserum to the virus A / Murmansk / 890/88 - up to a titer twice as high as the titer of this strain with homologous antiserum,
Окончательный анализ диагностической активности вирусов гриппа А/Ленинг- рад/325/88 и прототипного вируса А/Тэй- вань/1/86 был выполнен на материале, обследовани в РТГА сывороток детей и взрослых, переболевших ОРЗ и вакцинированных ИГВ при использовании диагно- стикумов, приготовленных из указанных штаммовThe final analysis of the diagnostic activity of influenza A / Leningrad / 325/88 viruses and the prototype A / Teivan / 1/86 virus was carried out on the material examined in HGV sera of children and adults who had had ARI and vaccinated IVI using diagnostic prepared from these strains
Оказалось, что диагностическа актив ность s РТГА у предлагаемого штамма вл етс наиболее высокой1 использование вируса А/Ленинград/325/88 в сравне ии со штаммом А/Тайвань/1/86 позволило увеличить частоту вы вл емых сероконвер- сий на 3,6-5,8%. При этом у детей частота диагностировани при использовании предлагаемого штамма увеличивалась почти в 2 раза (12,5 и 6,7% соответственно). У детей вакцинированных ИГЕ, процент вы вл емых сероконверсий увеличивалс на 22,2% (70,3 и 48,1% соответственно)It turned out that the diagnostic activity of s RTGA in the proposed strain is the highest. The use of A / Leningrad / 325/88 virus in comparison with A / Taiwan strain / 1/86 increased the incidence of seroconversion by 3.6%. 5.8%. At the same time, in children, the frequency of diagnosis with the use of the proposed strain increased almost 2 times (12.5 and 6.7%, respectively). In children vaccinated with IHE, the percentage of seroconversions detected increased by 22.2% (70.3% and 48.1%, respectively)
(табл 3).(Table 3).
Средние геометрические титров антител в сыворотках переболевших гриппом и вакцинированных были выше при использовании штамма А/Ленинград/325/88The geometric mean antibody titers in the sera of the influenza-infected and vaccinated were higher with the use of strain A / Leningrad / 325/88
(H1N1) в сравнении с близким по антигенной структуре штаммом А/Тайвань/1/86 (H1N1), что свидетельствует о более высокой авидности предлагаемого штамма. Использование штамма А/Ленинград/325/88 (H1N1) позволило существенно повысить эффективность диагностики современного гриппа, вызванного вирусами с антигенной структурой A(H1N1).(H1N1) in comparison with the strain A / Taiwan / 1/86 (H1N1), which is close in antigenic structure, indicating a higher avidity of the proposed strain. The use of strain A / Leningrad / 325/88 (H1N1) has significantly increased the efficiency of diagnosis of modern influenza caused by viruses with antigenic structure A (H1N1).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU904788265A SU1698288A1 (en) | 1990-02-01 | 1990-02-01 | Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU904788265A SU1698288A1 (en) | 1990-02-01 | 1990-02-01 | Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum |
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| Publication Number | Publication Date |
|---|---|
| SU1698288A1 true SU1698288A1 (en) | 1991-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU904788265A SU1698288A1 (en) | 1990-02-01 | 1990-02-01 | Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum |
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| SU (1) | SU1698288A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8748174B2 (en) | 2004-12-23 | 2014-06-10 | Medimmune, Llc | Non-tumorigenic MDCK cell line for propagating viruses |
| US8846032B2 (en) | 2006-09-15 | 2014-09-30 | Medimmune, Llc | MDCK cell lines supporting viral growth to high titers and bioreactor process using the same |
| RU2547587C2 (en) * | 2008-09-24 | 2015-04-10 | Медиммун, Ллк | Methods for cell culture, virus replication and purification |
-
1990
- 1990-02-01 SU SU904788265A patent/SU1698288A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Соминина А.А., Лисон Т.П., Корчанова Н.Л. Выбор диагностического штамма вируса гриппа А(Н1). -Сб. Трудов ВНИИ гриппа, 1977, 23, с, 62-68. Соминина А.А., Войцеховска Е М., Зо- щенкова Н.Я. и др. Использование реакции радиального гемолиза дл индикации антител к поверхностным антигенам вируса гриппа при оценке пост вакцинального иммунитета. - Микробиологический журнал. Киев, 1989,51. № 4, с. 54-59. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8748174B2 (en) | 2004-12-23 | 2014-06-10 | Medimmune, Llc | Non-tumorigenic MDCK cell line for propagating viruses |
| US8846032B2 (en) | 2006-09-15 | 2014-09-30 | Medimmune, Llc | MDCK cell lines supporting viral growth to high titers and bioreactor process using the same |
| RU2547587C2 (en) * | 2008-09-24 | 2015-04-10 | Медиммун, Ллк | Methods for cell culture, virus replication and purification |
| US9085753B2 (en) | 2008-09-24 | 2015-07-21 | Medimmune, Llc | Methods for cultivating cells, propagating and purifying viruses |
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