SU1532970A1 - Method of modelling male genital tuberculosis - Google Patents

Abstract

This invention relates to medicine, namely phthisiology. The goal is to improve the accuracy of the method. To this end, testosterone propionate is administered to the animal for 6-8 days, then the vein of the uterine plexus is collapsed with a collagen hemostatic sponge. After that, 0.1 mg of a pure tuberculosis microbacterial culture is injected into the vein and the vein is re-embolized with a collagen hemostatic sponge. The application of the method allows to increase the accuracy of modeling of male genital organs tuberculosis in comparison with the prototype due to the absence of dissemination of tuberculosis infection.

Description

This invention relates to medicine, namely to phthisiology.

The goal - an approximation to the clinical course.

The method is carried out as follows.

Prior to the operation, daily hormonal preparation of a mature male rabbit with an oily 1% testosterone propionate solution in a dose of 0, 1 ml subcutaneously, 1 time per day for 6-8 days, is carried out. Hypertrophy of the genital organs and dilatation of the uviform plexus observed at the same time improve the technical capabilities of subsequent manipulations. Before the operation in aseptic conditions, a polyvinyl chloride microcatheter of 0.8 mm0 diameter is prepared. The distal end of the catheter is introduced in series: a strip of collagen hemostatic sponge (about 0 , 1 mm), a pure culture of Mycobacterium tuberculosis at a dose of 0.1 mg, a second strip of collagen hemostatic sponge of similar length. A wire mandrel is inserted into the proximal microcatheter up to the first strip of collagen sponge. Then, under general anesthesia with 3.5 ml of a 1% solution of promeolar in aseptic conditions, a cut is made along the anterior-lateral surface of the scrotum. Pbslojno open the shell of the egg, mobilize the organs of the scrotum. Under a binocular loupe using magnifications (x 3.3), a vein corresponding to the diameter of the vascular microcatheter is isolated and isolated from the parafunique of the tissue of the uviform plexus. The vessel is taken into two ligatures, the proximal section is tied up with its first ligature. In the distal direction, after opening the vein, it catheterizes it to a natural obstacle. After this, the mandrin performs embolization.

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Condemn two collagen hemostatic emboli, between which a pure mycobacterium tuberculosis culture exists. Subsequently, the micro, atheter is removed from the mandrin, and at the same time the second ligature ligates the vein below the opening of the vessel. Nodal catgut sutures are applied to the parafuniqueral fatty tissue. Q The wound is sutured in layers of catgut by you tightly.

The method is confirmed by the following examples.

Example 1. Rabbit No. 22 15 Weight 3 kg 100 g. The floor is a male, the breed is a gray chinchilla.

Hormone preparation of a 1% testosterone propionate solution — 0.1 ml subcutaneously, 6 days prior to infection. 20 Rabbit is fixed to the machine in position on the back, Wool is removed in the scrotum area. Anesthesia - intramuscularly with 3.5 ml of a 1% solution of pro-medol. The skin of the scrotum is treated twice with 25 for 1 & n iodonate solution. The surgical field is limited to sterile wipes.

Preparation of the endovascular catheter (under aseptic conditions): a strip of collagen hemostatic sponge 1 mm in length was inserted successively into a 0.8 mm diameter microcatheter, a pure Mycobacterium tuberculosis culture in dose O, 1 mg, the second strip of collagen hemostatic sponge 1 mm long, wire mandrin was inserted into the opposite section of the microcatheter up to the first strip of collagen sponge

On the anterior-lateral surface of the scrotum, a 2-cm-long skin incision was made on the right. The testicle, its prim D5 and the spermatic cord are mobilized. Under the binocular loupe (magnification x 3.3), a branch of the uviform venous plexus with a diameter of 1.2 mm is highlighted. Last taken on two CQ catgut ligatures. The vein is proximally transected, and distally opened with microsurgical scissors and rolled up to 5 mm. The mandrin of the catheter was held distal 1 mm (double embolization itself + infection with a Mycobacterium tuberculosis culture). Mandible catheter is slowly removed with simultaneous ne35

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vein of the second ligature below the open vein. Superimposed nodular catgut sutures on parafunicular tissue. The skin wound is sutured with nodal catgut sutures tightly and treated with iodonate.

After 2 months the weight reached 3 kg 130g. The behavior of the rabbit - no change. A moderate temperature increase, slight hyperemia in the scrotal area on the right. The rabbit was removed from the experiment with a solution of intravenous sodium thiopental. “Pathological-anatomical dissection: the lungs are not macroscopically modified, the kidneys are macroscopically not modified, the genitals: to the right is tuberculous epididymitis with a transition to the right eye. Histological examination: clusters of tuberculous foci with a granulation shaft of epithelioid, lymphoid cells and giant Pirogov-Langns macrophages are determined in the epididymis of the right egg. In the center of the lesions - a cheesy necrosis. Tuberculous lesions go to the side of the testicle. In the prostate gland, the organs of the scrotum on the left, pathological changes in layer-by-layer sections were not revealed.

Example 2. Rabbit No. 38. Weight 3 kg 500 g "Male sex, breed - gray chinchilla. In the preoperative period, daily hormonal preparation of an intact male rabbit with an oily 1% testosterone propionate solution in a dose of 0.1 ml subcutaneously is performed. , Once a day for 8 days. In this case, moderate hypertrophy of the genital organs and the expansion of the veins of the form of the plexus plexus are observed, which improves the technical possibilities of subsequent manipulations. Before the operation under aseptic conditions, a microcatheter is prepared with a diameter of 0.8 mmo. A strip is inserted into the distal end of the catheter; collagen hemostatic sponge, pure culture of Mycobacterium tuberculosis in a dose of 0.1 mg, the second strip of collagen hemostatic sponge. A mandrin is introduced into the proximal microcatheter up to the first strip of collagen sponge. Then, under general dehydration {3.5 ml of a 1% solution of promedol), under aseptic conditions, a cut is made along the anterior-lateral surface of the scrotum. Layer-open shell ichka. Mobilized.

ichko, its appendage and spermatic cord. Under a binocular loupe using magnification (x 3.3), a vein corresponding to the diameter of the vascular microcatheter is detected and separated from the parafunicular fatty tissue of the uviform plexus. The vessel is taken on two ligatures, tied up; proximal division of his first ligature. In the distal direction, after opening the vein, it catheterizes it to a natural obstacle. After that, the mandrin produces embolization of the vessel by two collagen hemostatic emboli, between which there is a pure mycobacterium tuberculosis culture. Subsequently, the microcatheter with mandrin is removed and, at the same time, the second ligature ties the vein below the vessel opening site. Nodular catgut sutures are placed on the parafunicular fatty tissue. The wound is sutured with stratified catgut sutures tightly

After 3 months weight reached 3.5 kg. Behavior - no change, seal in the area of the epididymis 0.5x0.3 cm, local temperature increase and moderate swelling of the scrotal skin on the right.

Postmortem anatomy:. kidneys macroscopically not changed: genitals: right - tuberculosis

 the scrotum on the right cannot be differentiated, so there is a pronounced caseous necrosis with epithelioid lymphoid cells and giant macrophages (Pirogov – Langhans cells) along the periphery. In the prostate gland there are tuberculosis foci (cavities) with necrotic contents, epics

10 telioid and lymphoid cells on the periphery.

In the left uterine plexus, a typical tuberculous focus consisting of epithelioid,

15 Lymphoid cells and macrophages Pyrogov - Langkhansa.

The application of the method will allow to increase the accuracy of modeling the male tuberculosis of the male genital organs

20 with the prototype due to the absence of dissemination of tuberculosis infection „

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Claims (1)

Invention Formula A method of modeling male genital organs tuberculosis by locally infecting an animal with mycobacterium tuberculosis culture, which differs from the fact that testosterone is administered to an animal within 6–8 days in order to approach the clinical course due to the absence of dissemination of tuberculosis infection. orchiepididymitis with caseous division of propionate, then embolize the vein pronounced spasm with scrotal skin and cicatricial paraprocess. The transition of the tuberculous process to the prostate gland and the left vas deferens and the left uterine plexus. Histological examination: organs 40 uterine plexus with a collagen hemostatic sponge, vein 0.1 mg of pure mycobacterium tuberculosis culture is injected, and then the vein is re-embolized with a vascular plexus “  the scrotum on the right cannot be differentiated, so there is a pronounced caseous necrosis with epithelioid lymphoid cells and gigantic macrophages (Pirogov-Langhans cells) along the periphery. on the periphery. In the left uterine plexus, a typical tuberculous focus consisting of epithelioid, 5 lymphoid cells and macrophages of Pirogov - Langhans. The application of the method will improve the accuracy of modeling of male genital organs tuberculosis compared with. with the prototype due to the lack of dissemination of tuberculosis infection „ Invention Formula A method for simulating tuberculosis of the male genital organs by locally infecting the animal with a Mycobacterium tuberculosis culture, characterized in that, in order to approach the clinical course due to the absence of dissemination of tuberculosis infection, testosterone is administered to the animal within 6-8 days uterine plexus with a collagen hemostatic sponge, 0.1 mg of a pure culture of mycobacterium tuberculosis is injected into the vein, and then re-embolized the vein of the uviform plexus