SU1331887A1 - Apparatus for growing microorganisms - Google Patents

Abstract

The invention relates to the medical, microbiological and food industries, namely to Appaii (L 00 co oo 00 7;

Description

Ratam for growing microorganisms under sterile conditions with intensive foaming. The purpose of the invention is to reduce the entrainment of cultured liquid with exhausted air and to increase the efficiency of foaming. The apparatus for growing microorganisms contains a tank, a mixing device installed therein, aerators and venturi tubes for supplying a foam-air mixture to the aerators. Each aerator is equipped with a device for circulating foam into the culture fluid containing a chamber (K) 9 for collecting foam, which is fixed for movement along the skirt 15. At the shell 15, the nozzle 16 is also fixed for movement, counter nuts 17. each shell 15 with po1

The invention relates to the medical, microbiological and food industries, namely, apparatus for growing microorganisms under sterile conditions with intensive foaming.

The purpose of the invention is to reduce the entrainment of culture liquid with exhaust air and improve the effectiveness of the foam.

Fig. 1 schematically shows an apparatus for growing microorganisms, a longitudinal section; FIG. 2 shows the node I in FIG. in FIG. 3, node II in FIG. 2; figure 4 - node III in Fig, 1; figure 5 is a view of And figure 4; Fig. 6 shows a section B-B in ph. g. 3 (when all the holes on the disks are aligned) in Fig. 7 is the same when the upper disk is rotated.

The device for growing microorganisms contains a tank 1, a mixing device 2 with turbine agitators 3, aerators 4 with disks 5 fixed with a gap in relation to the flanges 6, a Venturi pipe 7 for supplying foam-air mixture to the aerators 4 and fitting 8 for exhaust air.

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the nut 18 is fastened to the pipe (T) 19 for the supply of sterile air. The outlet of each T 19 is located above the K 9 of the foam intake, and the T 19 is provided in the outlet with two disks 20 and 21 with holes 22, 23, 24 and 25 arranged one above the other to place the gas flow in two parts, one of which serves to dampen the foam, and the other - for aeration. The lower disk 21 is connected to a nozzle 16 placed in K 9 by means of a shell 15, and the upper one is rotatably mounted to change the flow area of the holes 22 and 23. To 9 the foam intake is provided with a cover 26 with a horizontally positioned end wall gas flow directed to quenching the foam, 1 hp f-ly, 7 ill.

Each aerator 4 is provided with a device for circulating foam into the culture fluid, which contains a ca-chamber 9 for foam collection, formed by truncated cones 10 and 11, conjugated with large bases, and having openings 12 for the passage of foam. The cone 10 is rotatably fixed with a cone 13 with windows 14 equal in size to the windows 12, the Chamber 9 is fixed so that it can move along the sidewall 15. The nozzle 16 is also movably fixed to the side shell 15, the position of which is fixed by means of a lock nut 17. Each shell 15 is fastened with a nut 18 to a pipe 19 for supplying sterile air. The outlet of each pipe 19 is located above the foam intake chamber 9 and the pipe is provided in the outlet with two disks 20 and 21 with openings 22-25 placed one above the other for section 5

Neither the gas flow into two parts, one of which serves to quench the foam, and the other for aeration. The lower disk 21 is connected to a nozzle 16 placed in the chamber 9 by means of a shell 15,

and the upper 20 is rotatably mounted to change the flow area of the holes 22 and 23.

The foam intake chamber 9 is provided with a cap 26 with a horizontally positioned end wall, which serves as a reflector 27 of the gas flow directed to damping the foam through the annular gap 28 formed by the nut 18 and the wall of the shell 15, and the adjustable gap 29. The holes 22 of the upper disc 20 are oval shaped in such a way that the air is supplied to the circulation of the foam — it is constant and does not stop at the moment of turning the disk 20 to align the holes 23 and 25 to supply part of the air to the damping of the foam.

The disk 20 has a shank 30 with a threaded hole 31 dp fastening the rod 32, which serves to rotate the disk. The twisting of the stem 32 in the threaded hole 31 is prevented by the lock nut 33. The stem 32 has a square cross-section and a thread 34 for fixing to it with a nut 35 a handle 36 with a switch 37. Sealing the stem 32 is provided with a stuffing box from the housing 38, fixed with the help of flange 39 and gasket 40 to flange 41 of pipe 19, grundbook 42 and stuffing box 43. The handle 36 is rotated manually or using any actuator, for example, an electromagnet (not shown). To ensure that the ring is attached and the drive mechanism is attached to the handle, a shoulder 44 and an oval hole 45 are made. Two stops 46 are fixed to the flange 39 of the stuffing box 38, which fixes the handle in two positions, corresponding to or sterile feeding. air only for foam recirculation, or for foam recirculation and defoaming.

In order to control the recycling of foam and defoaming, the device has electrodes 47 and 48 of the lower and upper limit levels of the foam, which give a signal to the drive mechanism of the handle 36 when operating in automatic mode, or a light signal during manual control.

The device works as follows.

After washing and sterilization of all communications, the nutrient medium is poured into the tank, the appropriate microorganism culture is inoculated. Then, the stirrer 3 is turned on and sterile air is supplied through pipes 19 for transporting oxygen to the nutrient medium and mixing it further, thus achieving the necessary conditions for mass exchange. In the initial position, the upper disk 20 is rotated so that the holes 25 in the lower disk 21 are blocked and the oval holes 22 are aligned with the holes 24. In the absence of foam, the air through the nozzle 16, the chamber 9 and the Venturi 7 completely flow through the aerators 4 with disks 5 for sparging at a given speed. The foam, which is formed in the upper part of the tank 1 during the operation of the apparatus, through the windows 12 is sucked into the chamber 9 by the energy of compressed sterile air exhausted from the nozzle 16, is fed into the venturi tube, where, due to a drop in static pressure, the foam bubbles are destroyed, and it together with the air through aerators 4 enters the culture fluid. The vacuum in chamber 9 is formed by increasing the speed of air movement as it flows out of social 16 and moves in the Venturi tube 7, where the air velocity increases even more and its static pressure drops. The magnitude of the vacuum is controlled by exposing the nozzle 16 in a certain position by means of a lock nut 7 on the sidewall 15, as well as by means of the live section of the windows 12, the value of which can be adjusted by rotating the cone 13 with the windows-14. Bubbles of foam, which are destroyed in the venturi 7 and acquire a droplet structure, are intensively enriched with oxygen due to pressure drops and air velocities.

When the foam exceeds a predetermined level and a danger of ejection occurs through the nozzle 8, the signal from the upper level electrode 48 is received either from the actuator or from the light level - manual control -. The upper disc 20 is rotated by the stem 32 and the handle 36 to a position in which the apertures 32 coincide with the apertures 25 of the lower disc 21 and the air through the ring

The target gap 28, the adjustable gap 29 strikes the reflector 27, changes direction and with a given speed enters the internal cavity of the container 1 parallel to the level of the liquid. The foam is captured by the air flow and breaks against the walls of the tank 1. The liquid that separates from the liquid flows downwards, and the air goes out through the fitting 8. At the same time as the air is supplied to the defoaming part of it is due to the fact that the holes 22 and 24 are constantly aligned. rest on foam circulation and aeration. Due to the fact that the holes 22 are made oval at the moment of switching the air flow, the air supply to the aeration and recirculation of the foam does not stop.

The actual air consumption for defoaming and its speed are governed by the size of the gap 29.

When lowering the foam below the lower limit level, the signal from the electrode 47 comes in, the upper disk 20 rotates and the holes 25 of the lower disk 21 overlap. The defoaming air is not supplied, but continues to flow to the aeration and recycling of the foam.

The proposed design of the apparatus for growing microorganisms eliminates the possibility of picking foam from the apparatus, which ensures the stability of its operation during abundant foaming, increases the efficiency and productivity of the process. In addition, it is possible to increase the filling ratio of the apparatus with a culture liquid, eliminate the need to use chemical defoaming, which is directly and not indirect.

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improves its performance

Claims (2)

1. An apparatus for growing microorganisms containing a container, pipes for supplying sterile air to aeration, and aerators equipped with devices for circulating foam into the culture fluid, each of which contains a foam collecting chamber having openings, a venturi tube, sub-. connected to it and to the aerator; a nozzle placed in the chamber, characterized in that, in order to reduce entrainment of the culture fluid with the exhaust air and to increase the efficiency of defoaming,. the outlet of each pipe for the supply of sterile air is located above the foam intake chamber and the pipe is provided in the outlet part with two disks with holes arranged one above the other to divide the gas flow into two parts, one of which serves to extinguish the foam and the other for at the same time, the lower disk is connected to a nozzle placed in the chamber with a shell, and the upper one is mounted for rotation to change the flow area of the holes, and the foam collection chamber is fitted with a crush horizontally end wall, which serves as a reflector of a part of the gas flow directed to quenching the foam. 2. The apparatus according to claim 1, characterized in that the opening of the upper disc has an oval shape. Blown 9lL2.1 Jf 39 one Fy 36 .S B-B 20 22 6 20 23 FIG. 7 Editor I.Egorova Compiled by G. Loshkareva Tehred V.Kadar Proofreader M.Sharoshi 3770/23 Circulation 499 Subscription VNIIPI USSR State Committee on affairs, inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., d.4 / 5 Production and printing company, Uzhgorod, Projecto st., 4