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SU1090719A1 - Method for determining in vitro threshold concentration of membranetoxic effect of chemicals - Google Patents

Method for determining in vitro threshold concentration of membranetoxic effect of chemicals Download PDF

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Publication number
SU1090719A1
SU1090719A1 SU823499924A SU3499924A SU1090719A1 SU 1090719 A1 SU1090719 A1 SU 1090719A1 SU 823499924 A SU823499924 A SU 823499924A SU 3499924 A SU3499924 A SU 3499924A SU 1090719 A1 SU1090719 A1 SU 1090719A1
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SU
USSR - Soviet Union
Prior art keywords
chemicals
vitro
threshold concentration
membranetoxic
determining
Prior art date
Application number
SU823499924A
Other languages
Russian (ru)
Inventor
Александр Семенович Шпигель
Игорь Петрович Королюк
Василий Алексеевич Данилин
Алевтина Николаевна Климина
Original Assignee
Куйбышевский медицинский институт им.Д.И.Ульянова
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Application filed by Куйбышевский медицинский институт им.Д.И.Ульянова filed Critical Куйбышевский медицинский институт им.Д.И.Ульянова
Priority to SU823499924A priority Critical patent/SU1090719A1/en
Application granted granted Critical
Publication of SU1090719A1 publication Critical patent/SU1090719A1/en

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Abstract

СПОСОБ ОПРЕДЕЛЕНИЯ IN. IVITRO ПОРОГОВОЙ КОНЦЕНТРАЦИИ МЕМБРАНОТОКСИЧЕСКОГО ДЕЙСТВИЯ ХИМИЧЕСКИХ ВЕЩЕСТВ с регистрацией биолюминесценции , отличающийс  тем, что, с целью повышени  точнойти и упрощени  способа, эритроциты человека инкубируют in vitro с химическими веществами концентраций от М до 1 М и регистрируют выход АТФ из клеток хемилюминесцентным методом.METHOD OF IDENTIFICATION IN. IVITRO threshold concentration MEMBRANOTOKSICHESKOGO OF CHEMICALS with registration bioluminescence, characterized in that, in order to increase tochnoyti and simplify the process, human erythrocytes incubated in vitro with chemicals concentrations from M to 1 M and recorded yield of ATP from cells chemiluminescent method.

Description

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Claims (1)

СПОСОБ ОПРЕДЕЛЕНИЯ IN. 'VITRO ПОРОГОВОЙ КОНЦЕНТРАЦИИ МЕМБРАНОТОКСИЧЕСКОГО ДЕЙСТВИЯ ХИМИЧЕСКИХ ВЕЩЕСТВ с регистрацией биолюминесценции, отличающийся тем, что, с цёлью повышения точности и упрощения способа, эритроциты человека инкубируют in vitro с химическими веществами концентраций от 1х10*иМ до 1 М и регистрируют выход АТФ из клеток хемилюминесцент;ным методом. (11),METHOD OF DEFINITION IN. 'VITRO THRESHOLD CONCENTRATION OF MEMBRANOTOXIC ACTION OF CHEMICALS with registration of bioluminescence, characterized in that, in order to increase the accuracy and simplify the method, human red blood cells are incubated in vitro with chemicals of concentrations from 1x10 * and M to 1 Mf and the cell yield is A ; by the new method. (eleven),
SU823499924A 1982-10-14 1982-10-14 Method for determining in vitro threshold concentration of membranetoxic effect of chemicals SU1090719A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU823499924A SU1090719A1 (en) 1982-10-14 1982-10-14 Method for determining in vitro threshold concentration of membranetoxic effect of chemicals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU823499924A SU1090719A1 (en) 1982-10-14 1982-10-14 Method for determining in vitro threshold concentration of membranetoxic effect of chemicals

Publications (1)

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SU1090719A1 true SU1090719A1 (en) 1984-05-07

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SU823499924A SU1090719A1 (en) 1982-10-14 1982-10-14 Method for determining in vitro threshold concentration of membranetoxic effect of chemicals

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SU (1) SU1090719A1 (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
1. Методил установлени допустимых уровней воздействи профессиональных вредностей. Доклад Комитета экспертов Всемирной Организации . Здравоохранени с участием МОТ, Сери технических докладов 601, Женева, 1978. 2. Патент US 4246340, кл. С 12Q1/66, 20.01.81 (прототип). *

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