SU1051118A1 - Apparatus for culturing microorganisms - Google Patents

Abstract

A MACHINE FOR CULTIVATION OF MICROORGANISMS, containing a column tank, placed along the height of a perforated bulkhead, equipped with drain pipes, an aerator located in the lower part of the tank, a device for extinguishing, installed in the upper part of the latter, and a circulating pipe, characterized by that, in order to increase the specific productivity of the apparatus, devices for cooling and separating the gas-and-gas mixture are installed between the partition walls, consisting of manifolds placed horizontally in the stalled plane near the vessel wall and around the standpipe and interconnected by hollow plates, which have a cross section along the vertical of a zigzag-i different shape.

Description

 The invention relates to equipment for the microbiological industry and can be used as an apparatus for growing microorganisms.

The closest to the proposed technical essence is an apparatus for growing microorganisms, containing a column tank, spaced perforated partitions equipped with drain pipes, an aerator located in the lower part of the tank, a device for antifoam and installed in the upper part of the latter and circulating circulating pipe | 1

However, in the apparatus there are no devices for thermal stabilization of the process of expression and insufficient efficiency of renewing the contact surface of the liquid and gas phases, which reduces the specific productivity of the apparatus in terms of biomass,

The purpose of the invention is to increase the specific productivity of the apparatus ..

The goal is achieved by the fact that, in the apparatus for growing microorganisms, we have a tank in the column, perforated bulkheads placed along the height of the tank, equipped with drain pipes, an aerator located in the lower part of the tank, a defoamer installed on the upper part of the latter, and a circulator device, between the partitions, devices for cooling and separating the gas-liquid mixture, consisting of reservoirs located in a horizontal plane near the vessel wall and wok yr. a downspout and interconnected hollow plates / having a zigzag shape in cross section along the vertical.

Fig. 1 schematically shows an apparatus for growing microorganisms, a slit; in fig. 2 shows section A-A in FIG. 1; in fig. 3 shows a section BB in FIG. 2; FIG. 4 is a section bb of FIG. 2

The device for growing microorganisms contains a column tank 1, perforated partitions 2 placed along its height, which are equipped with drain pipes 3. There is an aerating device in the lower part of the tank, including a dispersing perforated partition 4 and nozzles 5 for supplying air. Rotary mechanical defoamers 6 and a manifold 7 for supplying a chemical defoaming agent. On the axis of the tank 1, a downward circulation pipe 8 is installed with a heat-exchange jacket E. Thermal Exchange

The jacket 9 has nozzles 10 and 11 for supplying and discharging coolant. Above the partitions 2 at a distance of 0.12 D, where D is the tank, devices 12 are installed for cooling and separating the gas-liquid mixture, consisting of collectors 13-15, located in horizontal planes near the wall of tank 1 and around the standpipe 8 and

0 interconnected hollow plates 16 / having a zigzag shape in cross section along the vertical. The collectors 13 and 14 have connections 17 and 18 for feeding and

5 removal of heat carrier. The tank is equipped with nozzles, respectively, for feeding the nutrient medium, removal of the finished product and exhaust air.

Q The machine works as follows

Nutrient medium and seed are loaded into the tank 1, and air is fed through the nozzles 5 and the dispersing wall 5. The air, mixing with the culture liquid, forms a gas-liquid mixture, which in the space between the wall of the tank 1 and the descending circulation pipe 8 successively passes through the perforated partitions 2 and the device 12 for cooling and separating the latter. At the same time, perforated p. Partitions 2 destroy the enlarged

5 | air bubbles and disperse the gas-liquid flow, and the zigzag channels between the plastic plates 16 partially separate the gas-liquid mixture into gas and liquid.

0 The gas phase is pushed to the centerline of the zigzag channels, and the liquid to their walls. The alternating dispersion and partial separation of the gas-liquid flow contributes to the effective renewal of the surface of the interfacial contact. In addition, a decrease in the gas content at the walls of the zigzag channels improves the heat exchange conditions between the gas-rich culture fluid and the coolant.

In the upper part of the tank 1, the gas-liquid stream is separated into liquid and gas. The liquid through the downward circulation pipe 8 is returned to the zone of the dispersing partition 4, and the exhaust gas through the defoamers b and the pipe 21 is discharged from the apparatus. Pmshlr main

Circulation circuit through channel fused tubes 3 with reflectors in the device. Circuit channel circuits are organized, which provides additional intensification

mass transfer.

As a result of comparative tests of models of the prototype and the proposed apparatus for growing L-lysine, it was found that the specific

AT

16 The latter’s capacity is 40% higher, with a product yield of 25 and 35 kg / m, respectively.

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Claims (1)

DEVICE FOR GROWING MICROORGANISMS, containing a column container, perforated partitions arranged along the height of the container, equipped with drain pipes, an aerator located in the lower part of the container, a defoaming device installed in the upper part of the container, and a lowering circulation pipe that distinguishes the fact that, in order to increase the specific productivity of the apparatus, devices for cooling and separating the gas-liquid mixture, consisting of collectors placed in the horizon, are installed between the partitions Flax plane at the vessel wall and around the dipleg and interconnected by hollow plates having a cross-sectional vertical zigzag shape. I URWI gg