SK279564B6 - Method of microbial lipids preparation during the production of l-lactic acid - Google Patents
Method of microbial lipids preparation during the production of l-lactic acid Download PDFInfo
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- SK279564B6 SK279564B6 SK347-95A SK34795A SK279564B6 SK 279564 B6 SK279564 B6 SK 279564B6 SK 34795 A SK34795 A SK 34795A SK 279564 B6 SK279564 B6 SK 279564B6
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- lactic acid
- microbial lipids
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- 150000002632 lipids Chemical class 0.000 title claims abstract description 28
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 title claims abstract description 26
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 230000000813 microbial effect Effects 0.000 title claims description 12
- 238000000034 method Methods 0.000 title claims description 10
- 238000002360 preparation method Methods 0.000 title description 2
- 241000235527 Rhizopus Species 0.000 claims abstract description 6
- 240000005384 Rhizopus oryzae Species 0.000 claims abstract description 6
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims abstract description 5
- -1 n-alkanes Chemical class 0.000 claims abstract description 3
- 150000007524 organic acids Chemical class 0.000 claims abstract description 3
- 235000005985 organic acids Nutrition 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 22
- 230000004151 fermentation Effects 0.000 claims description 22
- 241000233866 Fungi Species 0.000 claims description 7
- QYDYPVFESGNLHU-UHFFFAOYSA-N elaidic acid methyl ester Natural products CCCCCCCCC=CCCCCCCCC(=O)OC QYDYPVFESGNLHU-UHFFFAOYSA-N 0.000 claims description 7
- QYDYPVFESGNLHU-KHPPLWFESA-N methyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC QYDYPVFESGNLHU-KHPPLWFESA-N 0.000 claims description 7
- 229940073769 methyl oleate Drugs 0.000 claims description 7
- 239000002028 Biomass Substances 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 2
- 229930195729 fatty acid Natural products 0.000 claims description 2
- 239000000194 fatty acid Substances 0.000 claims description 2
- 239000002253 acid Substances 0.000 abstract description 3
- 230000006372 lipid accumulation Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 125000001931 aliphatic group Chemical group 0.000 abstract 1
- 150000002148 esters Chemical class 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 14
- 239000002609 medium Substances 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 8
- 239000004310 lactic acid Substances 0.000 description 7
- 235000014655 lactic acid Nutrition 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 229920005862 polyol Polymers 0.000 description 5
- 150000003077 polyols Chemical class 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000411 inducer Substances 0.000 description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 description 1
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 229960002733 gamolenic acid Drugs 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000012499 inoculation medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000003170 nutritional factors Nutrition 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Vynález sa týka spôsobu výroby mikrobiálnych kyselín, predovšetkým kyseliny γ-linolénovej v procese výroby kyseliny mliečnej pomocou vláknitých húb rodu Rhizopus.The invention relates to a process for the production of microbial acids, in particular γ-linolenic acid, in a process for producing lactic acid by means of filamentous fungi of the genus Rhizopus.
Doterajší stav technikyBACKGROUND OF THE INVENTION
Polynenasýtené mastné kyseliny sa biotechnologický pripravujú povrchovými, submerznými kultiváciami, prípadne polosuchými fermentáciami tukotvomých mikroorganizmov, medzi ktoré patria predovšetkým vláknité huby z rodov Mucor, Mortierella, Phycomyses, Aerobasidium, Rhizopus a pod. [Ratledge, C., Boulton, C.A.: In Comprehensive Biotechnology, Vol.3, str. 983, Pergamon, Oxford 1985]. Tieto fermentácie prebiehajú 5-14 dní na bohatých komplexných médiách pri vysokých nárokoch na sterilitu procesu, pričom po separácii biomasy je vyfermentované médium odpadom. Tento takt, aj napriek vysokým dosahovaným obsahom lipidu v naprodukovanej biomase, predstavuje mimoriadne ekonomické zaťaženie týchto výrob.The polyunsaturated fatty acids are prepared biotechnologically by surface, submerged cultures or semi-dry fermentations of fat-forming microorganisms, which mainly include filamentous fungi of the genera Mucor, Mortierella, Phycomyses, Aerobasidium, Rhizopus and the like. [Ratledge, C., Boulton, C.A .: In Comprehensive Biotechnology, Vol. 3, p. 983, Pergamon, Oxford 1985]. These fermentations take place for 5-14 days on rich complex media with high demands on process sterility, and after separation of the biomass the fermented medium is waste. This cycle, in spite of the high lipid contents achieved in the biomass produced, represents a particularly economic burden on these productions.
Vláknité huby rodu Rhizopus, druhy arrhizus a oryzae, patria aj medzi producentov kyseliny mliečnej. Oproti baktériám produkujúcim túto kyselinu, sú menej náročné na výživové faktory a navyše, majú vysokú stereošpecificitu (produkujú výhradne L(+)-fonnu kyseliny mliečnej). Tieto vláknité huby produkujú kyselinu mliečnu v aeróbnom režime fermentácie pri vysokom pomere N:C v pH v rozmedzí 5,5 - 7,0, čo sú podmienky vhodné i na tvorbu lipidu v mycéliu.Filamentous fungi of the genus Rhizopus, arrhizus and oryzae, are also producers of lactic acid. Compared to bacteria producing this acid, they are less demanding on nutritional factors and moreover, they have a high stereospecificity (they produce exclusively L (+) - lactic acid form). These filamentous fungi produce lactic acid in the aerobic mode of fermentation at a high N: C ratio in the pH range of 5.5 - 7.0, which are also suitable conditions for lipid formation in the mycelium.
Podstata vynálezuSUMMARY OF THE INVENTION
Podstatou spôsobu výroby mikrobiálnych lipidov pri výrobe kyseliny mliečnej pomocou vláknitých húb rodu Rhizopus podľa vynálezu je, že po skončení produkcie kyseliny L-mliečnej sa fermentačné médium kultivuje pri teplote 25 až 45 °C počas 12 až 72 hodín pri pH 5,0 až 7,0, biomasa sa odseparuje, premyje vodou a mikrobiálne lipidy sa izolujú bežnými postupmi. Ako produkčné mikroorganizmy je možné použiť druhy Rhizopus arrhizus alebo Rhizopus oryzae, výhodne kmeňa Rhizopus arrhizus R-41 (CCM 8109). Výhodné je pridať do fermentačného média v priebehu produkcie laktátu, alebo po jej skončení látky stimulujúce akumuláciu lipidov, a ktoré môžu slúžiť ako uhlíkatý zdroj pri syntéze lipidov, ako n-alkány výhodne frakcií CioCi8> metylestery mastných kyselín, výhodne metyloleátu, organické kyseliny, výhodne kyseliny citrónovej, prípadne polyoly, výhodne glycerolu. Tento režim následnej kultivácie nespôsobuje úbytok už naprodukovaného laktátu, čo je nevyhnutné z hľadiska dosiahnutia optimálnych výťažkov laktátu po ukončení fermentácie.The process of producing microbial lipids in the production of lactic acid by means of the filamentous fungi of the genus Rhizopus according to the invention is that after the L-lactic acid production is complete, the fermentation medium is cultured at 25-45 ° C for 12-72 hours at pH 5.0-7. The biomass is separated, washed with water and the microbial lipids isolated by conventional procedures. Rhizopus arrhizus or Rhizopus oryzae, preferably Rhizopus arrhizus R-41 (CCM 8109), can be used as the production microorganism. It is preferred to add to the fermentation medium during or after the production of lactate lipid accumulating stimulants and which can serve as a carbon source in lipid synthesis, such as n-alkanes, preferably C 10 -C 18 fatty acid methyl esters, preferably methyl oleate, organic acids, preferably citric acid or polyols, preferably glycerol. This post-cultivation regimen does not cause the loss of already produced lactate, which is necessary in order to achieve optimal lactate yields after fermentation.
V priebehu kultivácie sa hodnota pH udižiava prídavkom alkalický reagujúcich roztokov, hlavne NaOH, KOH, NH4OH, výhodne CaCO3 a Ca(OH)2- Fermentačné médium sa intenzívne mieša a prevzdušňuje výhodne 0,2-0,8 objemu vzduchu na objem fermentačného média.During cultivation, the pH is controlled by the addition of alkaline reacting solutions, in particular NaOH, KOH, NH 4 OH, preferably CaCO 3 and Ca (OH) 2. The fermentation medium is vigorously stirred and aerated preferably 0.2-0.8 volume of air per volume of fermentation medium .
Po ukončení produkcie laktátu neobsahuje vyfermentované médium žiadne bežné sacharidické substráty, ktoré sa používajú na produkciu kyseliny mliečnej (glukóza, sacharóza a pod.), ale len polyoly (manitol, glycerol a pod.) v koncentráciách 2-20 g/1, ktoré vznikajú počas produkcie laktátu. Tieto polyoly sa v ďalšom priebehu kultivácie znovu utilizujú a slúžia ako zdroj na syntézu lipidov. Výhodné je pridať do fermentačného média v priebehu produkcie laktátu, alebo po jej skončení látky stimulujúce akumuláciu lipidov, ako n-alkány (Cjo-Cjs),metylestery mastných kyselín, výhodne metyloleátu, prípadne polyoly, výhodne glycerolu v koncentráciách 2-20g/l podľa typu substráte. Koncentrácia sa volí tak, aby po ukončení fermentácie bola zbytková koncentrácia v médiu minimálna a nebránila efektívnej izolácii laktátu a mastných kyselín.After lactate production is terminated, the fermented medium does not contain any conventional carbohydrate substrates that are used to produce lactic acid (glucose, sucrose, etc.), but only polyols (mannitol, glycerol, etc.) at 2-20 g / l that are produced during lactate production. These polyols are re-utilized in the further course of the culture and serve as a source for lipid synthesis. It is advantageous to add to the fermentation medium during or after the production of lactate lipid-stimulating substances, such as n-alkanes (Cjo-C18), fatty acid methyl esters, preferably methyl oleate or polyols, preferably glycerol, at concentrations of 2-20g / l, substrate type. The concentration is chosen such that after fermentation the residual concentration in the medium is minimal and does not impede the effective isolation of lactate and fatty acids.
Fermentácia sa predlžuje až po dosiahnutie maximálnej akumulácie lipidu v mycéliu vláknitej huby. Napr. po 48 hodinách fermentácie sa prekonvertuje 15 % roztok sacharózy na kyselinu L-mliečnu s výťažkom 90 - 95 %, pričom výťažnosť lipidu je okolo 1,0 g/1. Po prídavkumetyloleátu (10g/l) a následnej 48 hod. kultivácii sa obsah lipidu v mycéliu zdvojnásobí, pričom výťažnosť laktátu sa nemení.Fermentation is prolonged until maximum lipid accumulation in the mycelium of the filamentous fungus is reached. E.g. after 48 hours of fermentation, a 15% sucrose solution is converted to L-lactic acid in a yield of 90-95%, with a lipid yield of about 1.0 g / l. After addition of methyl oleate (10g / l) followed by 48 hours. by cultivation, the lipid content of the mycelium is doubled, while the lactate yield does not change.
Mikrobiálne lipidy je možné pripraviť po separácii biomasy z vytermentovaného média a následnou extrakciou organickými rozpúšťadlami, nadkritickými plynmi a inými izolačnými technikami.Microbial lipids can be prepared after separation of biomass from fermented medium and subsequent extraction with organic solvents, supercritical gases and other isolation techniques.
Príklady uskutočnenia vynálezuDETAILED DESCRIPTION OF THE INVENTION
Príklad 1Example 1
Pripraví sa inokulačné médium, ktoré obsahuje v 1 dm3 vodovodnej vody, 15 gD-glukózy, lOg melasy, lOg kukuričného škrobu, 4,0g (NH4)2SO4, l,2g KH2PO4,An inoculation medium containing in 1 dm3 of tap water, 15 gD-glucose, lOg of molasses, log of corn starch, 4.0 g (NH 4) 2 SO 4, l, 2 g of KH2PO4,
0,25g MgSO4.7H2O, 0,05g ZnSC>4.7H20, 0,01g0.25g MgSO 4 .7H 2 O, 0.05g ZnSC> 4.7H 2 O, 0.01g
Fe2(SC>4)3.9H2O a 2,0 g kukuričného výluhu. Po 24 hodinovej kultivácii 0,12 dm3 média v 0,5 dm3 kultivačných bankách na rotačnej trepačke pri 30 °C sa zo spór vláknitej huby Rhizopus arrhizus CCM 8109 pripraví vegetatívne inokulum, ktoré sa použije na inokuläciu 4,5 dm3 produkčného média vo fermentore s objemom 7 dm3 v množstve 5 % objemových. Produkčné médium obsahuje v 1 dm3 vodovodnej vody 120g D-glukózy, 2,0g (NH4)2SO4, 0,25g KH2PO4, 0,3g MgSO4.7H2O, 0,04 g ZnSO4.7H2O, 0,01g Fe2(SO4)3.9Il20 a 0,5 g kukuričného výluhu. Fermentácia sa uskutočňuje pri teplote 35 °C, vzdušnení 3 dm3 vzduchu/min., miešaní 450 ot./min. a pretlaku 0,03 MPa. Vznikajúca kyselina mliečna sa neutralizuje prídavkom CaCO3 alebo Ca(OH)2 a hodnota pH sa tak udržuje na hodnote 5,5-6,0. Po 48 hodinách kultivácie obsahuje fermentačné médium kyselinu L-mliečnu v koncentrácii 86 g/1 (t. j. 95,5 % teoretickej hodnoty) a zbytková koncentrácia glukózy je menšia ako 0,1 g/1. V médiu sa naprodukovali polyoly (glycerol, manitol) v koncentrácii 8 -10 g/1. Fermentačné médium sa mieša (400 ot./min.) a vzdušní (2 dm3 vzduchu/min.) pri teplote 33°C ďalších 48 hodín pri hodnote pH 5,5. Po ukončení fermentácie (96 hod.) sa biomasa odseparuje od tekutého podielu, premyje sa vodou a lipidy sa izolujú z mycélia extrakciou organickými rozpúšťadlami, nadkritickými plynmi, prípadne inými bežnými postupmi (Floch, J., Less, M., Stanley, G.: J. Biol. Chem. 226, 1957, s. 497 - 509). Objemová výťažnosť lipidu je 2,1 g/1, výťažnosť kyseliny mliečnej je 94,2 % teoretickej hodnoty.Fe 2 (SC> 4) 3 .9H 2 O and 2.0 g corn steep liquor. After 24 hours cultivation of 0.12 dm 3 medium in 0.5 dm 3 culture flasks on a rotary shaker at 30 ° C, a vegetative inoculum is prepared from the spores of the filamentous fungus Rhizopus arrhizus CCM 8109 and used to inoculate 4.5 dm 3 production medium. in a fermenter of 7 dm 3 in an amount of 5% vol. The production medium contains 120g D-glucose, 1gm 3 of tap water, 2.0g (NH4) 2SO4, 0.25g KH2PO4, 0.3g MgSO4.7H2O, 0.04g ZnSO4.7H2O, 0.01g Fe2 (SO4) 3.9 120 and 0.5 g of corn extract. The fermentation is carried out at a temperature of 35 ° C, aeration of 3 dm 3 air / min, stirring at 450 rpm. and overpressure of 0.03 MPa. The resulting lactic acid is neutralized by the addition of CaCO 3 or Ca (OH) 2, maintaining the pH at 5.5-6.0. After 48 hours of culture, the fermentation broth contains L-lactic acid at a concentration of 86 g / l (i.e. 95.5% of the theoretical value) and a residual glucose concentration of less than 0.1 g / l. Polyols (glycerol, mannitol) were produced in the medium at a concentration of 8 -10 g / l. The fermentation medium is stirred (400 rpm) and aerated (2 dm 3 air / min) at 33 ° C for an additional 48 hours at pH 5.5. After fermentation (96 h), the biomass is separated from the liquid, washed with water and the lipids isolated from the mycelium by extraction with organic solvents, supercritical gases, or other conventional methods (Floch, J., Less, M., Stanley, G. J. Biol Chem 226, 1957, 497-509). The lipid volume yield is 2.1 g / l, the lactic acid yield is 94.2% of the theoretical value.
Príklad 2Example 2
Príprava inokula, zloženie médií je analogické ako v príklade 1. Po vyčerpaní glukózy a ukončení produkciePreparation of the inoculum, the composition of the media is analogous to that of Example 1. After glucose is exhausted and production is stopped
SK 279564 Β6 laktátu (48 hod.) sa do média pridá metyloleát v koncentrácii 5 gri ako induktor syntézy lipidov. Ďalej sa fermentácia prevádza analogicky ako v príklade 1. Objemová výťažnosť lipidu sa dosiahne 2,7 g/1, čas fermentácie a výťažky laktátu sú analogické ako v príklade 1.Lactate (48 hr) was added to the medium at 5 gri methyl oleate as an inducer of lipid synthesis. Further, the fermentation is carried out analogously to Example 1. The lipid volume yield is 2.7 g / l, the fermentation time and lactate yields are analogous to Example 1.
Príklad 3.Example 3.
Analogicky ako v príklade 2 s tým, že na stimuláciu syntézy lipidu sa namiesto metyloleátu použije prídavok frakcie n-alkánov C]()-C]X v koncentrácii 5 g/1 ako induktora syntézy lipidov. Objemová výťažnosť lipidu je 3.1 g/1.Analogously to Example 2, the addition of a 5 g / l n-alkane fraction as an inducer of lipid synthesis is used instead of methyl oleate to stimulate lipid synthesis. The lipid volume recovery is 3.1 g / l.
Príklad 4.Example 4.
Analogicky ako v príklade 2 s tým, že na stimuláciu syntézy lipidu sa namiesto metyloleátu použije prídavok kyseliny citrónovej vo forme sodnej soli v koncentrácii 5 g/1 ako induktora syntézy lipidov. Objemová výťažnosť lipiduje 2,7 g/1.Analogously to Example 2, the addition of citric acid sodium salt at a concentration of 5 g / l was used instead of methyl oleate to stimulate lipid synthesis as an inducer of lipid synthesis. The bulk yield lipid is 2.7 g / l.
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| SK347-95A SK279564B6 (en) | 1995-03-16 | 1995-03-16 | Method of microbial lipids preparation during the production of l-lactic acid |
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1995
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