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SG11202100725YA - Biosynthetic platform for the production of cannabinoids and other prenylated compounds - Google Patents

Biosynthetic platform for the production of cannabinoids and other prenylated compounds

Info

Publication number
SG11202100725YA
SG11202100725YA SG11202100725YA SG11202100725YA SG11202100725YA SG 11202100725Y A SG11202100725Y A SG 11202100725YA SG 11202100725Y A SG11202100725Y A SG 11202100725YA SG 11202100725Y A SG11202100725Y A SG 11202100725YA SG 11202100725Y A SG11202100725Y A SG 11202100725YA
Authority
SG
Singapore
Prior art keywords
cannabinoids
production
prenylated compounds
biosynthetic
platform
Prior art date
Application number
SG11202100725YA
Inventor
James Bowie
Meaghan Valliere
Tyler Korman
Nicholas Woodall
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of SG11202100725YA publication Critical patent/SG11202100725YA/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02Oxygen as only ring hetero atoms
    • C12P17/06Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42Hydroxy-carboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/03Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with oxygen as acceptor (1.2.3)
    • C12Y102/03003Pyruvate oxidase (1.2.3.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008Phosphate acetyltransferase (2.3.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/010394-Hydroxybenzoate polyprenyltransferase (2.5.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202100725YA 2018-08-01 2019-08-01 Biosynthetic platform for the production of cannabinoids and other prenylated compounds SG11202100725YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862713348P 2018-08-01 2018-08-01
PCT/US2019/044752 WO2020028722A1 (en) 2018-08-01 2019-08-01 Biosynthetic platform for the production of cannabinoids and other prenylated compounds

Publications (1)

Publication Number Publication Date
SG11202100725YA true SG11202100725YA (en) 2021-02-25

Family

ID=69232031

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202100725YA SG11202100725YA (en) 2018-08-01 2019-08-01 Biosynthetic platform for the production of cannabinoids and other prenylated compounds

Country Status (12)

Country Link
US (2) US11479760B2 (en)
EP (1) EP3830581A4 (en)
JP (1) JP2021532747A (en)
KR (1) KR20210049805A (en)
CN (1) CN112789505B (en)
AU (1) AU2019314484B2 (en)
BR (1) BR112021001450A2 (en)
CA (1) CA3107544A1 (en)
IL (1) IL280442B2 (en)
MX (1) MX2021001065A (en)
SG (1) SG11202100725YA (en)
WO (1) WO2020028722A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2021001065A (en) * 2018-08-01 2021-06-23 Univ California Biosynthetic platform for the production of cannabinoids and other prenylated compounds.
CA3128196A1 (en) 2019-01-30 2021-08-06 Genomatica, Inc. Recovery, decarboxylation, and purification of cannabinoids from engineered cell cultures
CA3130763A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
US12540315B2 (en) 2019-04-12 2026-02-03 Renew Biopharma, Inc. Compositions and methods for using genetically modified enzymes
CA3142619A1 (en) * 2019-06-06 2020-12-10 Genomatica, Inc. Olivetolic acid cyclase variants and methods for their use
US20220411766A1 (en) * 2019-10-03 2022-12-29 Renew Biopharma, Inc. Compositions and methods for using genetically modified orthologous enzymes
JP7527038B2 (en) * 2019-12-26 2024-08-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Biosynthetic platforms for the production of cannabinoids and other prenylated compounds
CN111286509B (en) * 2020-03-20 2021-02-26 天津法莫西生物医药科技有限公司 Alkene reductase mutant and coding gene and application thereof
EP3901256A1 (en) * 2020-04-21 2021-10-27 Synbionik GmbH Optimized production of cbga from olivetol acid and geranyl pyrophosphate via synnphb
CN113355300B (en) * 2020-08-05 2022-04-01 深圳蓝晶生物科技有限公司 Aromatic isopentenyl transferase mutant, construction method of recombinant bacterium for expression of mutant and recombinant bacterium constructed by mutant
WO2022133213A1 (en) * 2020-12-18 2022-06-23 Debut Biotechnology, Inc. Cell-free production of geranyl pyrophosphate from glycerol in a cell-free manufacturing system
EP4347800A1 (en) 2021-05-26 2024-04-10 Invizyne Technologies, Inc. Prenyltransferase variants with increased thermostability
CN113584089B (en) * 2021-07-01 2023-11-24 嘉兴欣贝莱生物科技有限公司 Use of prenyltransferase to catalyze the synthesis of cannabinol or cannabidiol acid
CN114621982B (en) * 2022-03-16 2023-11-07 嘉兴欣贝莱生物科技有限公司 Biosynthesis method of geranyl diphosphate and application of geranyl diphosphate in preparation of cannabis compounds
CN116024111B (en) * 2022-12-03 2025-09-05 中国科学院深圳先进技术研究院 A cannabinoid-producing microbial cell and its construction method and application
CN116622784B (en) * 2023-02-14 2024-03-01 黑龙江八一农垦大学 Application of cannabidiolic acid synthase
CN117363593B (en) * 2023-11-14 2024-07-09 北京大学深圳研究生院 Multienzyme cascade catalytic system and application thereof in enantiomer-kaurene synthesis
CN117512033B (en) * 2023-12-21 2024-04-19 山东三元生物科技股份有限公司 Method for simultaneously producing D-tagatose and D-psicose from glucose

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2204300B1 (en) * 2002-07-18 2005-05-01 J. URIACH & CIA S.A. NEW DERIVATIVES OF 2,4-DIHYDROXIBENZOIC ACID.
CN101137663A (en) * 2005-01-28 2008-03-05 索尔克生物学研究所 Novel aromatic prenyltransferases, nucleic acids encoding same and uses thereof
DE102010011601A1 (en) * 2010-03-16 2011-09-22 Eberhard-Karls-Universität Tübingen New prenyltransferase from fungi, where its enzymatic activity is independent in the presence of magnesium ions, useful for the biotechnological production of prenylated compounds, e.g. 3-dimethylallyl-2,7-dihydroxynaphthalene
WO2017015429A2 (en) * 2015-07-21 2017-01-26 The Regents Of The University Of California Glucose metabolism with molecular purge valve
WO2018200888A1 (en) * 2017-04-27 2018-11-01 Regents Of The University Of California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
EP3762487A4 (en) * 2018-03-08 2022-03-23 Genomatica, Inc. PRENYLTRANSFERASE VARIANTS AND METHODS FOR PRODUCING PRENYLATED AROMATIC COMPOUNDS
WO2019183152A1 (en) * 2018-03-19 2019-09-26 Renew Biopharma, Inc. Compositions and methods for using genetically modified enzymes
MX2021001065A (en) * 2018-08-01 2021-06-23 Univ California Biosynthetic platform for the production of cannabinoids and other prenylated compounds.
US12540315B2 (en) * 2019-04-12 2026-02-03 Renew Biopharma, Inc. Compositions and methods for using genetically modified enzymes
CN113355300B (en) * 2020-08-05 2022-04-01 深圳蓝晶生物科技有限公司 Aromatic isopentenyl transferase mutant, construction method of recombinant bacterium for expression of mutant and recombinant bacterium constructed by mutant

Also Published As

Publication number Publication date
US20210309975A1 (en) 2021-10-07
US11479760B2 (en) 2022-10-25
IL280442B1 (en) 2025-02-01
US20230193221A1 (en) 2023-06-22
JP2021532747A (en) 2021-12-02
US12180516B2 (en) 2024-12-31
EP3830581A4 (en) 2022-07-27
WO2020028722A1 (en) 2020-02-06
CN112789505B (en) 2024-04-09
IL280442B2 (en) 2025-06-01
AU2019314484A1 (en) 2021-02-25
AU2019314484B2 (en) 2025-12-18
CN112789505A (en) 2021-05-11
MX2021001065A (en) 2021-06-23
BR112021001450A2 (en) 2021-04-27
CA3107544A1 (en) 2020-02-06
KR20210049805A (en) 2021-05-06
EP3830581A1 (en) 2021-06-09
WO2020028722A9 (en) 2020-04-09
IL280442A (en) 2021-03-01

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