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SE8101665L - POLYPEPTIDFRAMSTELLNING - Google Patents

POLYPEPTIDFRAMSTELLNING

Info

Publication number
SE8101665L
SE8101665L SE8101665A SE8101665A SE8101665L SE 8101665 L SE8101665 L SE 8101665L SE 8101665 A SE8101665 A SE 8101665A SE 8101665 A SE8101665 A SE 8101665A SE 8101665 L SE8101665 L SE 8101665L
Authority
SE
Sweden
Prior art keywords
gene
fused
polypeptide
eukaryotic
region
Prior art date
Application number
SE8101665A
Other languages
Unknown language ( )
Swedish (sv)
Inventor
L P Guarente
M Ptashne
T M Roberts
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of SE8101665L publication Critical patent/SE8101665L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compositions and methods featuring, in one aspect, a method of providing a fused gene having a promoter located an optimal distance from the translation start site, the method including providing a fused gene having a region of a gene coding for a eukaryotic or prokaryotic polypeptide fused to a region of a gene coding for an assayable polypeptide, inserting a portable promotor at varying distances in front of the translation start site, cloning the fused genes into microorganisms, selecting those producing the greatest amount of assayable polypeptide, and reconstituting the gene for the eukaryotic or prokaryotic protein.
SE8101665A 1980-03-17 1981-03-16 POLYPEPTIDFRAMSTELLNING SE8101665L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13115280A 1980-03-17 1980-03-17

Publications (1)

Publication Number Publication Date
SE8101665L true SE8101665L (en) 1981-09-18

Family

ID=22448125

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8101665A SE8101665L (en) 1980-03-17 1981-03-16 POLYPEPTIDFRAMSTELLNING

Country Status (11)

Country Link
JP (1) JPS56167698A (en)
AT (1) AT378004B (en)
BE (1) BE887991A (en)
CA (1) CA1198385A (en)
DE (2) DE3153267A1 (en)
ES (2) ES8205432A1 (en)
FR (1) FR2478125B1 (en)
GB (1) GB2071671B (en)
IT (1) IT1144705B (en)
NL (1) NL8101289A (en)
SE (1) SE8101665L (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (en) * 1980-12-31 1984-01-10 Ilkka Antero Palva FOERFARANDE FOER PRODUCERING AV ETT UTVALT AEGGVITEAEMNE OCH VID FOERFARANDET ANVAENDA REKOMBINANTPLASMIDVEKTORER
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
WO1983004262A1 (en) * 1982-05-25 1983-12-08 Brandeis University Method of producing protein fragments
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
JPS5939899A (en) * 1982-08-27 1984-03-05 Gakuzo Tamura Novel vector
EP0130074B1 (en) * 1983-06-27 1991-02-06 Genentech, Inc. Portable inducible control system, expression vectors containing them, microorganisms transformed with them, and their use in expressing exogenous protein
WO1985003522A1 (en) * 1984-02-08 1985-08-15 Cetus Corporation Monitoring and control systems for recombinant manipulations
US4923808A (en) * 1985-03-12 1990-05-08 Genentech, Inc. Method for identifying mutants secreting high levels of heterologous proteins
IL78775A (en) * 1985-05-15 1992-06-21 Biotech Australia Pty Ltd Oral vaccines
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
US5030576A (en) * 1986-04-30 1991-07-09 Genentech, Inc. Receptors for efficient determination of ligands and their antagonists or agonists
WO2016208385A1 (en) 2015-06-23 2016-12-29 富士フイルム株式会社 Electroacoustic conversion film and electroacoustic transducer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2008123B (en) * 1977-11-08 1982-09-08 Genentech Inc Method of means for microbial polypeptide expression
GR71912B (en) * 1978-08-11 1983-08-16 Univ California
NL8000127A (en) * 1979-01-15 1980-07-17 Harvard College METHOD FOR FORMING PROKARYOTIC OR EUKARYOTIC PROTEINS AND A GENERALLY COUPLED GENE.

Also Published As

Publication number Publication date
ATA122281A (en) 1984-10-15
GB2071671B (en) 1983-10-05
FR2478125B1 (en) 1985-07-05
GB2071671A (en) 1981-09-23
ES500397A0 (en) 1982-06-01
DE3153267A1 (en) 1985-08-14
FR2478125A1 (en) 1981-09-18
CA1198385A (en) 1985-12-24
IT8167359A0 (en) 1981-03-16
JPS56167698A (en) 1981-12-23
DE3110031A1 (en) 1982-01-07
IT1144705B (en) 1986-10-29
ES8205432A1 (en) 1982-06-01
ES8306177A1 (en) 1983-05-01
ES510007A0 (en) 1983-05-01
NL8101289A (en) 1981-10-16
AT378004B (en) 1985-06-10
BE887991A (en) 1981-07-16

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