[go: up one dir, main page]

SE2430062A1 - Efficient production of recombinant casein - Google Patents

Efficient production of recombinant casein

Info

Publication number
SE2430062A1
SE2430062A1 SE2430062A SE2430062A SE2430062A1 SE 2430062 A1 SE2430062 A1 SE 2430062A1 SE 2430062 A SE2430062 A SE 2430062A SE 2430062 A SE2430062 A SE 2430062A SE 2430062 A1 SE2430062 A1 SE 2430062A1
Authority
SE
Sweden
Prior art keywords
casein
microbial strain
recombinant
cell division
bioreactor
Prior art date
Application number
SE2430062A
Inventor
Johan Krakau
Original Assignee
Cassius Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cassius Ab filed Critical Cassius Ab
Priority to SE2430062A priority Critical patent/SE2430062A1/en
Priority to PCT/SE2025/050092 priority patent/WO2025170525A1/en
Publication of SE2430062A1 publication Critical patent/SE2430062A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/04—Animal proteins
    • A23J3/08—Dairy proteins
    • A23J3/10—Casein
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C11/00—Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/08—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing caseinates but no other milk proteins nor milk fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
    • A23J1/202—Casein or caseinates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/20—Proteins from microorganisms or unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a method for production of recombinant casein, wherein the method comprises the steps of: (a) adding a microbial strain to a first bioreactor, wherein said microbial strain has the capacity to express casein, and said microbial strain comprises means for deactivation of cell division; (b) culturing said microbial strain in the first bioreactor, wherein the cell division is active, thereby forming a continuously growing biomass comprising cells of said microbial strain; (c) deactivating cell division; (d) inducing expression of casein; (e) after sufficient expression of casein, lysing said cells, thereby forming a lysis broth, and separating said lysis broth so that casein can be separated into a casein containing phase; (f) treating the casein containing phase to induce formation of casein micelles; and (g) optionally further purifying and/or treating the casein micelles. In other aspects, the invention also relates to a bioreactor system, a recombinant casein, and products including a recombinant casein.
SE2430062A 2024-02-08 2024-02-08 Efficient production of recombinant casein SE2430062A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE2430062A SE2430062A1 (en) 2024-02-08 2024-02-08 Efficient production of recombinant casein
PCT/SE2025/050092 WO2025170525A1 (en) 2024-02-08 2025-02-07 Efficient production of recombinant casein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE2430062A SE2430062A1 (en) 2024-02-08 2024-02-08 Efficient production of recombinant casein

Publications (1)

Publication Number Publication Date
SE2430062A1 true SE2430062A1 (en) 2025-08-09

Family

ID=94688059

Family Applications (1)

Application Number Title Priority Date Filing Date
SE2430062A SE2430062A1 (en) 2024-02-08 2024-02-08 Efficient production of recombinant casein

Country Status (2)

Country Link
SE (1) SE2430062A1 (en)
WO (1) WO2025170525A1 (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10313863A (en) * 1997-03-19 1998-12-02 H S P Kenkyusho:Kk Escherichia coli mutant
US20190216106A1 (en) * 2016-08-25 2019-07-18 Perfect Day, Inc. Food products comprising milk proteins and non-animal proteins, and methods of producing the same
WO2020223700A1 (en) * 2019-05-02 2020-11-05 New Culture, Inc. Cheese and yogurt like compositions and related methods
WO2022098835A1 (en) * 2020-11-04 2022-05-12 New Culture, Inc. Dairy-like compositions and related methods
US20220251498A1 (en) * 2019-02-15 2022-08-11 Just-Evotec Biologics, Inc. Facilities and processes to produce biotherapeutics
WO2022226507A1 (en) * 2021-04-20 2022-10-27 Duke University Two-stage dynamic control over redox state improves cystolic expression of disulfide containing proteins in e. coli
WO2023151198A1 (en) * 2022-02-10 2023-08-17 江南大学 Casein micelle having compositional and structural features imitating human milk and preparation method therefor
WO2023235555A1 (en) * 2022-06-02 2023-12-07 Bee-Io Honey Technologies Ltd. Cultured buffalo milk production methods, systems, compositions and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6071718A (en) 1996-11-27 2000-06-06 Abbott Laboratories Methods of producing a recombinant protein
US6121421A (en) 1998-08-21 2000-09-19 Abbott Laboratories Methods for isolating recombinant β-casein
AU2022287200A1 (en) * 2021-06-01 2023-12-07 Standing Ovation Method for producing casein and uses thereof
WO2023133417A2 (en) * 2022-01-05 2023-07-13 Change Foods, Inc. Dairy-like compositions

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10313863A (en) * 1997-03-19 1998-12-02 H S P Kenkyusho:Kk Escherichia coli mutant
US20190216106A1 (en) * 2016-08-25 2019-07-18 Perfect Day, Inc. Food products comprising milk proteins and non-animal proteins, and methods of producing the same
US20220251498A1 (en) * 2019-02-15 2022-08-11 Just-Evotec Biologics, Inc. Facilities and processes to produce biotherapeutics
WO2020223700A1 (en) * 2019-05-02 2020-11-05 New Culture, Inc. Cheese and yogurt like compositions and related methods
WO2022098835A1 (en) * 2020-11-04 2022-05-12 New Culture, Inc. Dairy-like compositions and related methods
WO2022226507A1 (en) * 2021-04-20 2022-10-27 Duke University Two-stage dynamic control over redox state improves cystolic expression of disulfide containing proteins in e. coli
WO2023151198A1 (en) * 2022-02-10 2023-08-17 江南大学 Casein micelle having compositional and structural features imitating human milk and preparation method therefor
WO2023235555A1 (en) * 2022-06-02 2023-12-07 Bee-Io Honey Technologies Ltd. Cultured buffalo milk production methods, systems, compositions and uses thereof

Also Published As

Publication number Publication date
WO2025170525A1 (en) 2025-08-14

Similar Documents

Publication Publication Date Title
Panesar et al. Biotechnological approaches for the value addition of whey
Groot et al. Increase of substrate conversion by pervaporation in the continuous butanol fermentation
Silva et al. Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation
Escobar et al. Effect of agitation speed on the synthesis of Mucor miehei acid protease
Roukas et al. Production of lactic acid from deproteinized whey by coimmobilized Lactobacillus casei and Lactococcus lactis cells
Bhave et al. Expression of Vitreoscilla hemoglobin improves growth and levels of extracellular enzyme in Yarrowia lipolytica
US20160312252A1 (en) Compositions and methods for biodiesel production from waste triglycerides
Asada et al. Re-evaluation of hydrogen productivity from acetate by some photosynthetic bacteria
CN102311927A (en) Medium and method for high density fermentation of saccharomyces cerevisiae
Elsayed et al. Optimization of fed-batch cultivation strategy for extracellular α-amylase production by Bacillus amyloliquefaciens in submerged culture
SE2430062A1 (en) Efficient production of recombinant casein
Walker et al. Morphological and metabolic changes in the yeast Kluyveromyces marxianus var. marxianus NRRLy2415 during fermentation of lactose
Costa et al. Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation
Galvagno et al. Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol
GARG et al. Continuous production of citric acid by immobilized whole cells of Aspergillus niger
VIETH et al. Immobilized microbial cells in complex biocatalysis
CA1183091A (en) Microbiological oxidations
MX2023012698A (en) Single vessel expansion of lymphocytes.
Furlan et al. Optimization of pH, Temperature and Inoculum Ratio for the Production of β‐D‐Galactosidase by Kluyveromyces marxianus Using a Lactose‐free Medium
JPH0630593B2 (en) Method for industrial production of polyol by fermentation of sugars
CA3141341A1 (en) Methods and compositions for anaerobic bacterial fermentation
JPWO2021136689A5 (en)
López-y-López et al. Redirection of metabolism during nutrient feeding in fed-batch cultures of Bacillus thuringiensis
US6716617B1 (en) Fermentation method with continuous mass cultivation of ciliates (protozoa) for producing biogenous valuable substances
Nigam et al. Solid-state fermentation: An overview