SE1050280A1 - Framställt frö, som har emballeringsmaterial - Google Patents

Description

Within the seed blank by an end seal. There is a weakened spot in the end seal to allow the radicle end of the plant embryo to penetrate the end seal.

At has been discovered by the inventors of the present method that the rate of normal gerrnination of an embryo from such manufactured seeds Was unacceptably low.

Their analysis noted that only about 30% of embryos Within a study group of manufactured seeds produced a normal seedling having an expected root, cotyledons, etc.

The present inventors observed that embryos, when inserted into the cavity of the cotyledon restraint having a diameter larger than the diameter of the embryo itself causes the cotyledons to be reflexed during somatic embryo development. This results in only the tips of the cotyledons to contact the Walls of the cotyledon restraint cavity through Which nutrition is passed from the gametophyte to the embryo. The present inventors theorize that this caused the unacceptably low productivity of the manufactured seeds.

Thus, there is a need for a method of preparing a material for use in a manufactured seed that improves the productivity of the manufactured seed.

SUMMARY This summary is provided to introduce a selection of concepts in a Simplified form that are further described below in the Detailed Description. This summary is not intended to identify key features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.

A manufactured seed is provided. The manufactured seed includes a seed shell housing a nutritive media and a restraínt disposed within the seed shell. The restraint includes a cavity. The rnanufactured seed also includes an embryo disposed within the cavity and a till material disposed Within the cavity. The manufactured further includes a seal disposed on the seed Shell to seal the embryo Within the seed Shell.

DESCRIPTION OF THE DRAWINGS The foregoing aspects and many of the attendant advantages of this invention will become better understood by reference to the following detailed description, When taken in conjunction With the accompanying drawings, Wherein: FIGURE 1 is a side cross-sectional planar view of a manufactured seed having a packing material in accordance with certain aspects of the present disclosure.

WEYE\26366 APPDOC "z" 10 15 20 25 30 DETAILED DESCRIPTION A manufactured seed 20 having a fill material 120 constructed in accordance With certain aspects of the present disclosure may be best understood by referring to FIGURE l. The manufactured seed 20 includes a cylcap 22, a seed shell 24, nutritive media 26, such as a gametophyte, and a dead end seal 28. The seed shell 24 is suitably formed from a section of tubular material. In one embodiment, the seed shell 24 is a seetioned straw of frbrous material, such as paper. The sections of straw may be pre- treated in a suitable coating material, such as Wax.

In other embodirnents, the seed shell24 is formed from a tubular section of biodegradable, plastic material. One such material is a utilized polylatic acid ("PLA") and is sold by NAT-UR of Los Angeles, California. Another material within the scope of the disclosure is a poiycaprolactone ("PCL") mixture, such as Dow Tone P-787 (Dow Chemical Co., Midland, MI 48647) With a 1% Tegomer H SI6440 plasticizer (Degussa Goldschmidt Chemical Corp, 914 East Randolph Road, Hopewell, VA 23860).

Such biodegradable plastic tubes are sectioned into appropriate lengths for a manufactured seed. Further, such biodegradable plastic tubes may or may not require a Wax coating as such tubes are already resistive to environmental elements. lt should be apparent that although sectioning tubes is preferred, other embodiments, such as obtaining tubes of appropriate size for use as manufactured Seeds, are als vvithin the scope of the present disclosure.

The cylcap 22, also known as a restraint, is suitably rnanufactured from a porous material having a hardness strong enough to resist puncture or fracture by a genninating embryo, such as a ceramic or porcelain material, and includes an end Seal portion 30 and a cotyledon restraint portion 32. The cotyledon restraint portion 32 is suitably integrally or unitarily formed With the end seal portion 30. The cylcap 22 also includes a longitudinally extending cavity 34 extending through the end seal portion 30 and partially through one end of cotyledon restraint portion 32. The open end of the cavity 34 is known as a cotyledon restraint opening 36. The cavity 34 is sized to receive a plant embryo 42 therein.

In certain embocliments, as the cylcap 22 is suitably manufactured from a porous material, it may be desirable to coat the cylcap 22 With a barrier material to reduce the rate of Water loss and restrict or reduce microbiai entry. Such barriers include Wax, WEY'E\2636ü APPDOC '3 ' 10 -is 20 25 30 polyurethane, glaze, nail polish, and a coating sold by H.B. Fuller of Minneapolis, MN under the product name PD124.

The end seal portion 30 is suitably circular when viewed in a top planar view and includes sidewalls 38. Although Circular is the preferred embodiment of the end seal portion 30, other ernbodiments and shapes, such as polygonal, square, triangular, oval and other shapes, are also Within the scope of the present disclosure.

In the embodiment of FIGURE 1, the sideWalls 38 are defined by the thickness of the end seal portion 30 and has a diameter substantially equal to the inside diameter of the seed she1l24. In certain embodiments, the cylcap 22 is bonded to the seed shell 24 by heat. As a non-limiting example, during manufacturing, the cylcap 22 may be heated to a predetennined temperature, such that When the seed shell 24 and the cylcap 22 are co-joined, heat transferred between the cylcap 22 and the seed shell 24 causes either the seed shell 24, the cylcap 22, or both to melt, thereby bonding the two together. In other embodiments, the cylcap 22 and the primary end seal 44 are first heat Welded, then in a separate step the combined primary end seal-cylcap is reheated and the seed sheïl 24 is also heated and the two are joined. Other methods of bonding the cylcap 22 to the seed shell 24, such as a Wax bond or a hot glue melt, are also Within the scope of the present disclosure.

The sidewalis 38 may include a tapered portion 40. The tapered portion 40 may be a chamfer of one end of the end seal portion 30. The tapered portion 40 assists in assembling the cylcap 22 to the seed coat 24 during manufacturing. Although a tapered portion 40 is preferred, other ernbodirnents, such as a cylcap that does not include a tapered portion, are also Within the scope of the present disciosure. An embryo 42 is disposed within the cavity 34 and is suitably sealed therein by a live end seai 43.

The live end seal 43 includes a primary end seal 44 and a secondary end seal 21.

The primary end seal 44 is suitably formed from a PCL material described above and includes a centrally located opening S0. The opening 50 is sized to correspond to diameter of the cavity 34 of the cylcap 22 to permit a germinating embryo 42 to pass therethrough. The primary end seal 44 is suitably attached to the end seal portion 30 by a variety of methods, including glue or heat bonding.

As a non-limiting example, the primary end seal 44 is mated to a pre-heated cylcap 22, such that the opening 50 is located above the cavity 34. The heat welds or WEYE\26366 APPDOC "4"' 10 15 20 25 30 bonds the primary end seal 44 to the cyicap 22. It should be apparent that the primary end seal 44 may be attached to the cylcap 22 before or after the cylcap 22 is attached to the seed shell 24. Also, if the seed shell 24 is constructed from PCL, it is desirable but not necessary that the melt temperature of the primary end seal 44 and the seed shell 24 be similar. i As another non-limiting example of attaching the primary end seal 44 to the cylcap 22, includes an adhesive gasket. In this example, the primary end seal 44 is heat sealed or bonded to the cylcap 22 with the opening 50 co~axially aligned With the cavity 34. ln this process, a form is used to bend edges of the primary end seal 44 around the perirneter of the end seal portion 30 of the cylcap 22. If the melt temperature of the primary end seal 44 and the seed Shell 24 are different, then a low bloom cyanoacrylate is used as an adhesive gasket to bond the primary end seal 44 and the Seed shell 22, Heat is applied after the glue and is done so as to thin the glue seal by rnelting incongruities that typically occur when manufacturing the seed shell 24 and forming the adhesive joint. Thereafter, the cylcap 22, including the primary end seal 44, is attached to the seed shell 24. As noted above, this method is also suitable to a cylcap 22 that is already attached to the seed shell 24. Finally, the foregoing method of attaching a primary end seal 44 to a seed shell 24 may be used for heat Weld compatible or incompatible materials.

The secondary end seal 21 will now be described in greater detail. In that regard, the secondary end seal 21 is suitably formed from a Well-known sealing material, such as Parafilm®. The secondary end seal 21 is formed and attached to the primary end seal 44 by a well-known method, such as heat bonding or gluing. In some embodirnents, a sealing Wax may be used to facilitate bonding between the PCL and the Parafilrn. The secondary end sea121 also includes a predetermined burst strength to permit a germinating embryo 42 to penetrate through the live end Seal 44.

Still referring to FIGURE l, the tertiary seal 60 Will now be described in greater detail. The tertiary seal 60 and live end seal 43, as used in the present embodirnent, define an outer sealing layer and an inner sealing layer, respectively. Although the live end seal 43 has been described as including both a primary end seal 44 and a second end seal 21, it should be apparent that the disclosure is not intended to be so limited. As a WEYE\26366 APPDOC '5 ' 10 15 20 25 30 non-limiting example, the live end seal 43 may include only the secondary end seal 21 and, therefore, such embodiments are also Within the scope of the present disclosure.

The combination of the tertiary sea160 and live end seal 43 creates a sealing surface, Wherein the sealing layer, defined by the tertiary seal 60, is made from a predetermined material that degrades in structural integrity after a predetermined exposure to environmental conditions. The tertiary seal 60 also serves as an anti- microbial sealant to seal and protect around the embryo as the ernbryo germinates and ernerges from within the seed shell 24 and protects the cotyledon restraint cavity.

Suitable materials used to manufacture the tertiary seal 60 include Water soluble materials, Wax, environmentally degradable materials, and biodegradable materials.

Thus, such materials, as well as materials equivalent in structure and properties, are Within the scope of the present disclosure.

As noted above, the seed shell 24 and primary end seal 44 are suitably formed from a polyester material, such as biodegradable plastic. One method of preparing the material for use in making components, such as the seed shell 24 and primary end seal 44, of the manufactured seed 20 in accordance with the present disclosure includes obtaining strips of material, such as strips of plastic. Such plastics Within the scope of the appended claims includes biodegradable plastics, such as PCL, The strips of material are armealed using tvell-known annealing methods to improve dimensional stability. In one non-limiting example, strips of PCL are armealed at a temperature of about 63° C for a range of time substantially between 35 minutes and 90 minutes. ln one embodiment, the strips of PCL are armealed for 45 minutes. The annealing time is a time sufficient to render the strips of PCL dímensionally stable during heat Welding. Thus, it should be apparent that the temperature and time required for annealing is a function of the material and is Within the scope of one in the art. Further, it should be apparent that the strip of material need not be armealed. Such embodiments are also within the scope of the appended claims.

The strips of material, regardless if they have been annealed, are heat treated to remove toxins. ln this process, and for PCL, the strips of material are placed in an oven and heat treated at a temperature of about 55” C for a period of eight days. lt should be apparent that although heat treating the material at the specified temperature for the specific time period, it should be apparent that other temperatures and times are Within WEYEQ6366 APPDÛC ' 6' 10 15 20 25 30 the seope of the present disclosure. As a nondimiting example, the strips of material may be heated treated at a temperature that is approximately 10% below the melting temperature of the material. Further, the time for the heat treatment may be as little as a few hours. Thus, such times and ternperatures are also within the scope of the appended clairns.

The heat treated strips of material are also subjected to a sterilization process. In this process, the strips of material are submerged into a sterilization bath of bleach mixture for a sterilization soak period. One bleach mixture is 10% bleach and 90% Water. The sterilization soak period ranges substantially between 10 minutes and 40 minutes. In one non-Iimiting embodiment, the strips of material are soaked in the bleach mixture for a sterilization period of 30 minutes. After the sterilization soak period, the strips of material are subjected to a rinse period in deionized Water. The rinse period can last up and beyond 72 hours. Thereafier, the strips of material are air dry in sterile air to evaporate fluids. Finally, the strips of material are processed to make various components of the manufactured seed 20, such as the seed shell 24 and primary end seal 44.

Still referring to FIGURE 1, the fill material 120 will now be described in greater detail. Preferably, the till material 120 is an adsorbent, such as activated charcoal, Dowex resins, zeolites, alumina, clay, diatomaceous earth, silica gel, and Kieselguhr.

During assembly of the manufactured seed 20, the fill material 120 is deposited into the cavity 34 of the cylcap 22 in any manner known in the art, including manually. The fill material 120 is preferably, but not necessarily, deposited within the cavity 34 such that it substantially centers the embryo 42 within the cavity 34. Centering the embryo 42 within the cavity 34 increases the surface area of the embryo 42 in functional contact With the nutritive media 26. As used Within this detailed description and appended claims, the term "ñmctional contact" is intended to mean in a position Where the embryo 42 uptakes nutrients from the nutritive media 26.

The fill material 120 increases the surface area of the embryo 42 in functional contact with the nutritive media 26 by surrounding the embryo 42 and, thereby, providing multiple pathways for the nutrients from the nutritive media 26 to pass to the embryo 42.

Although it is preferred that the fill material 120 suhstantially center the embryo 42 Within the cavity 34, the embryo 42 need not be so positioned. The fill material 120 need WEYE\2636b APPDOC “7" 5 10 only position the embryo 42 Within the cavity 34 in any manner to place the embryo 42 into functional contact with the nutritive media 26. Further, it is not necessary for the fill material 120 to "surround" the ernbryo 42. As such, the fill material 120 can completely or partially surround the embryo 42.

In other embodiments Within the scope of the appended clairns, the fill material 120 need only till, either completely or partially, one or two sides of the space between the embryo 42 and the Walls of the cavity 34.

One method of preparing the till material 120 for insertion into the cavity 34 includes combining approximately 7.0 g of activated charcoal With 246 ml of a nutritíve media, such as KE64 media, to create a mixture.

KE64 media is set forth in Table l.

One such forrnulation of the Final Amount Medium Concentration to add for Component Ung!!! 300 ml NH4NÛ3 3Ûl .l 2.2 Iïll H3BO3 10.0 0.75 ml (NH4)2MoO4 0.06 CaCl2-2H20 299.2 1.00 ml KH2PO4 18000 7.5 ml MgSO4-7H2O l000.0 Mncmnzo 6.0 0-75 m1 ZnSO4-7H2O 0.8 CuCl2-2H2O 0.5 Ferric Citrate 60 mg/l 0.75 ml TABLE 1 WEYEQÖJÖS APPDOC 'S ' Thereafter, add 30 ml of a 50% sucrose solution and organic stocks are added to the mixture. One such organic stock formulations is set forth in Table 2.

Bing Ei_11_al Amount to add for Medium Concentration Concentration 300 ml comglete Comgonent mM gmgfl) media Myo-inositol 0.5549 100.0 3.0 ml Thiarnine-HC] 00030 1.0 Pyridoxine-HCI 0.0012 025 Nicotinic acid 0.008l 1.0 Riboflavin 0.0021 0.125 Ca-pantothenate 0.50 Biotin 0.0003 0.0010 Folio acid 08077 01250 L-asparagine 18255 106.7 3.0 ml L-glutamine 03646 266.7 L-Iysine-2HCl 07612 53.3 DL-serine 04631 80 L-proline 1.5310 53.3 L-arginine-HCI 0.4552 266.7 Urea 133200 800 L-valine 05983 53.3 3.0 ml L-alanine 0.2203 53.3 L~leucine 02448 80 L-threonine 03226 26.7 L-phenylalanine 0.1720 53.3 L-histidine 0.13 08 26.7 L-tryptophan 0.203 5 26.7 L-isoleucine 1.2930 26.7 L-methionine 0.71 00 26.7 L-glycine 0.0003 53.3 L-tyrosine 0.2242 53.3 0.75 ml L-cysteine 06098 26.7 0.75 ml T ABLE 2 Thereafter, the mixture is autoclaved for about 25 minutes. The mixture is filtered through well-known filter paper and the fill material 120 (eg, charcoal) is harvested from the filter paper. flowable matter.

WEY'E\26366 AFPDOC The harvested fill material 120 is then dried until it becomes While ilíustrative embodiments have been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the inventíon.

WEYE\26366 APPJJOC '10"

Claims (10)

CLAIMS The embodiments of the invention in Which an exelusive property or privilege is elaimed are defined as follows:

1. l. A manufactured seed, comprising: (a) a seed Shell housing a nutritive media; (b) a restraint disposed Within the seed Shell, the restraint having a eavity; (e) an emhryo disposed Within the cavity; (d) a fill material disposed within the eavíty; and (e) a seal disposed on the seed shell to seal the embryo within the seed shell.

2. The manufaetured seed of Claim I, Wherein the till material is disposed Within the cavity to place the embryo into functional contact With the nutritive media.

3. The manufactured seed of Claim 2, wherein the till material is an adsorbent.

4. The manufactured seed of Claim 2, wherein the filI material is charcoal.

5. The manufactured seed of Claim l, Wherein the fill material is a pretreated charcoal.

6. The manufactured seed of Claim l, Wherein the fill material is an adsorbent.

7. The manufactured seed of Claim 1, Wherein the fill material is charcoal.

8. The manufactured seed of Claim l, Wherein the fill material is a pretreated chareoal. WEY'E\26366 AFP DÛC _11-

9. The manufactured seed of Claim l, Wherein the fill material substantially centers the embryo Within the cavity.

10. The manufactured seed of Claim 1, Wherein the fill material substantially centers the embryo Within the cavity to increase a surface area of the embryo in functional contact with the nutritive media. vifavsxzszsfi Amanoc - l 2-