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RU92000528A - METHOD OF REPRODUCTION, EXPRESSION, DIAGNOSTICS AND CLONING OF NUCLEIC ACIDS IN IMMOBILIZED MEDIA - Google Patents

METHOD OF REPRODUCTION, EXPRESSION, DIAGNOSTICS AND CLONING OF NUCLEIC ACIDS IN IMMOBILIZED MEDIA

Info

Publication number
RU92000528A
RU92000528A RU92000528/13A RU92000528A RU92000528A RU 92000528 A RU92000528 A RU 92000528A RU 92000528/13 A RU92000528/13 A RU 92000528/13A RU 92000528 A RU92000528 A RU 92000528A RU 92000528 A RU92000528 A RU 92000528A
Authority
RU
Russia
Prior art keywords
nucleic acids
expression
reproduction
cell
cloning
Prior art date
Application number
RU92000528/13A
Other languages
Russian (ru)
Other versions
RU2048522C1 (en
Inventor
А.Б. Четверин
Е.В. Четверина
Original Assignee
Институт белка РАН
Filing date
Publication date
Application filed by Институт белка РАН filed Critical Институт белка РАН
Priority to RU9292000528A priority Critical patent/RU2048522C1/en
Priority claimed from RU9292000528A external-priority patent/RU2048522C1/en
Priority to US07/966,713 priority patent/US5616478A/en
Application granted granted Critical
Publication of RU2048522C1 publication Critical patent/RU2048522C1/en
Publication of RU92000528A publication Critical patent/RU92000528A/en

Links

Claims (1)

Изобретение относится к области молекулярной биологии и биотехнологии, а именно - к способам размножения, экспрессии, клонирования и диагностики нуклеиновых кислот в бесклеточных системах. Преимущественной областью использования является препаративный синтез индивидуальных нуклеиновых кислот, клонирование нуклеиновых кислот и обнаружение специфических нуклеиновых кислот в анализируемом материале. Целью изобретения является способ размножения и экспрессии нуклеиновых кислот, при котором потомство каждой молекулы (клон) и продукты экспрессии остаются в той зоне реакционного объема, где исходно находилась матричная молекула. Размножение и/или экспрессию нуклеиновых кислот осуществляют в среде, которую иммобилизуют путем использования органической и/или неорганической твердой основы, пронизывающей среду и имеющей пористую, волокнистую, сетчатую, капиллярную, складчатую или слоистую структуру, которая включает компоненты бесклеточной ферментной системы экспоненциального размножения нуклеиновых кислот и/или компоненты бесклеточной ферментной системы экспрессии нуклеиновых кислот. Предпочтительно использование твердой основы, способной к обратимым взаимодействиям с нуклеиновыми кислотами, а также использование иммобилизованной среды, сформованной в виде по крайней мере одного тонкого слоя. Способ позволяет клонировать нуклеиновые кислоты вне клетки, а также детектировать одиночные молекулы нуклеиновых кислот в исследуемом образце.The invention relates to the field of molecular biology and biotechnology, namely to methods of reproduction, expression, cloning and diagnosis of nucleic acids in cell-free systems. The preferred field of use is the preparative synthesis of individual nucleic acids, the cloning of nucleic acids, and the detection of specific nucleic acids in the analyzed material. The aim of the invention is a method of reproduction and expression of nucleic acids, in which the progeny of each molecule (clone) and expression products remain in the zone of the reaction volume, where the matrix molecule was originally located. The reproduction and / or expression of nucleic acids is carried out in a medium that is immobilized by using an organic and / or inorganic solid base that penetrates the medium and has a porous, fibrous, reticular, capillary, folded or layered structure that includes components of a cell-free enzyme system of exponential reproduction of nucleic acids and / or components of a cell-free nucleic acid expression system. It is preferable to use a solid base capable of reversible interactions with nucleic acids, as well as the use of an immobilized medium formed in the form of at least one thin layer. The method allows to clone nucleic acids outside the cell, as well as to detect single nucleic acid molecules in the sample under study.
RU9292000528A 1992-10-14 1992-10-14 Method of nucleic acid copying, method of their expression and a medium for their realization RU2048522C1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU9292000528A RU2048522C1 (en) 1992-10-14 1992-10-14 Method of nucleic acid copying, method of their expression and a medium for their realization
US07/966,713 US5616478A (en) 1992-10-14 1992-10-26 Method for amplification of nucleic acids in solid media

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU9292000528A RU2048522C1 (en) 1992-10-14 1992-10-14 Method of nucleic acid copying, method of their expression and a medium for their realization
US07/966,713 US5616478A (en) 1992-10-14 1992-10-26 Method for amplification of nucleic acids in solid media

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU93044928A Addition RU2114915C1 (en) 1993-09-14 1993-09-14 Method of identification of nucleic acid

Publications (2)

Publication Number Publication Date
RU2048522C1 RU2048522C1 (en) 1995-11-20
RU92000528A true RU92000528A (en) 1996-03-10

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Family Applications (1)

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RU9292000528A RU2048522C1 (en) 1992-10-14 1992-10-14 Method of nucleic acid copying, method of their expression and a medium for their realization

Country Status (2)

Country Link
US (1) US5616478A (en)
RU (1) RU2048522C1 (en)

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