RU86352U1 - DEVICE FOR EXPERIMENTS ON PROTEIN SOLUTIONS IN AC VARIABLE MAGNETIC FIELD - Google Patents

Abstract

1. A device for conducting experiments on protein solutions in an alternating magnetic field, including a container of diamagnetic material, where a protein solution based on substances having hydrogen bonds is placed, two electrodes are located in the vessel, connected to an oscilloscope, and next to it to form a magnetic field an inductor connected to the generator is installed. ! 2. The device according to claim 1, characterized in that their aqueous or aqueous-alcoholic solutions are used as protein solutions. ! 3. The device according to claim 1, characterized in that plastic or glass can be used as the diamagnetic material of the container.

Description

The claimed technical solution relates to biophysical experiments and observations of protein solutions located in a double electric layer in an alternating magnetic field, and can be widely used in biology, ecology, medicine, etc.

As a result of studies conducted in Russia and abroad over the past 20 years, it has been found that in liquids containing macromolecules and having the ability to form hydrogen bonds, formations are formed whose sizes range from nano to micro objects, which can be changed using electric or magnetic component of the physical field. In this regard, the problem arises of conducting experiments to obtain information about these changes.

From available sources of information, we do not know information about devices for conducting experiments on protein solutions in an alternating magnetic field in a double dielectric layer.

The technical task of the solution is to create a device that allows experiments on protein solutions when exposed to an alternating magnetic field.

This problem is solved due to the fact that the device for conducting experiments on protein solutions in an alternating magnetic field includes a container of diamagnetic material, where protein solutions based on substances having hydrogen bonds are placed. Two electrodes are located in the vessel, which are isolated from the liquid medium, are connected to the oscilloscope, and an inductor connected to the generator is installed next to it to form a magnetic field. Plastic or glass can be used as the diamagnetic material of the container, and their aqueous or aqueous-alcoholic solutions can be used as protein solutions.

The technical result of the proposed solution is to obtain the possibility of conducting experiments with protein solutions, allowing to study their properties, in particular, the possibility of generating electromagnetic waves in them and to evaluate the parameters of these vibrations.

Figure 1 schematically shows a device for studying protein solutions in an alternating magnetic field.

It consists of a conductometric cell 1, which includes a capacitance 2 with a protein solution with electrodes 3 immersed in it, which, insulated from the liquid medium, are electrically connected to the oscilloscope 4. An inductor 5 is installed next to the conductometric cell 1, electrically connected to the generator 6.

The device operates as follows. A protein solution based on substances having hydrogen bonds isolated from biological objects is poured into the capacity 2 of the conductometric cell 1. From generator 6, harmonic electrical vibrations of a frequency selected from a range of 0.5-70 Hz are applied to an inductor 5, as a result of which an alternating magnetic field is created with a voltage of 10 to 120 A / m, which acts on the solution in the conductometric cell 1. V as a result, with the help of the oscilloscope 4, one can observe broadband electrical oscillations and measure their parameters.

The authors conducted a series of experiments with some protein solutions of animal and vegetable origin in a double electric layer in an alternating magnetic field.

Example 1. An aqueous protein solution of soy - urease was placed in a conductometric cell. The temperature of the solution corresponded to the ambient temperature (+ 22 ° C). From generator 6 of type G3-118, harmonic electrical vibrations with a frequency of 0.5 Hz are applied to the inductor 5, as a result of which an alternating magnetic field of 10 A / m is generated, which acts on the solution located in the conductivity cell 1. On the oscilloscope 4, we observed Broadband electrical vibrations with a maximum amplitude of 30 mV and a frequency of 1 kHz to 300 MHz. This effect was observed for 2 seconds. When the field with the saved parameters turned on, the frequency of the repetition of the effect varied from 30 to 180 s. When the magnetic field was turned off immediately after the generation of electric oscillations was stopped and turned on again after 10 seconds, the effect of the appearance of oscillations with parameters similar to the primary inclusion was again observed.

Example 2. Similar to example 1 except that the frequency of harmonic electrical vibrations supplied to the inductor 5 was 20 Hz, and the intensity of the generated magnetic field was 50 A / m. As a result, broadband electrical oscillations were observed on the oscilloscope 4, with a maximum amplitude of 30 mV and a frequency of 1 kHz to 300 MHz. This effect was observed for 5 seconds. When the field with the saved parameters turned on, the frequency of the repetition of the effect varied from 30 to 200 s.

Example 3. Similar to examples 1 except that the frequency of harmonic electrical vibrations supplied to the inductor 5 was 28 Hz, and the intensity of the generated magnetic field was 100 A / m. As a result, broadband electrical oscillations were observed on the oscilloscope 4, with a maximum amplitude of 30 mV and a frequency of 1 kHz to 300 MHz. This effect was observed for 3 seconds. When the field with the saved parameters turned on, the frequency of the repetition of the effect varied from 30 to 160 s.

Example 4. Similar to example 1, except that we used a water-alcohol (2% alcohol) protein solution and the frequency of the magnetic field was 34 Hz.

Example 5. Similar to example 2, except that we used a water-alcohol (5% alcohol) protein solution and the frequency of the magnetic field was 40 Hz.

Example 6. Similar to example 3, except that we used a water-alcohol (7% alcohol) protein solution and the frequency of the magnetic field was 57 Hz.

Example 7. Similar to example 1, except that we used an aqueous solution of interferon (protein content 30%) and the frequency of the magnetic field was 62 Hz. As a result, broadband electrical oscillations were observed on the screen of the oscilloscope 4 for 5 s, the maximum amplitude of which was 20 mV, and the frequency ranged from 1 kHz to 200 MHz.

Examples 8 and 9 are similar to examples 2 and 3, except that a 30% aqueous solution of interferon was used and the frequency of the magnetic field was 70 Hz.

These examples are shown in table 1.

Table 1 No. Protein solution Valid field Result Type of protein solvent Field strength, A / m. Frequency Hz. Range of generated oscillations, MHz. Maximum amplitude of oscillations, mV Long .. generation, p. The frequency of generation, s. Period changed valid. fields, s. one urease water 10 0.5 0.001-300 thirty 2 30-180 10 2 urease water fifty twenty 0.001-300 thirty 5 30-200 10 3 urease water one hundred 28 0.001-300 thirty 3 30-160 10 four urease water-alcohol 2% 10 34 0.001-300 thirty 2 30-180 10 5 urease water-alcohol 5% fifty 40 0.001-300 thirty 5 30-200 10 6 urease water-alcohol 7% one hundred 57 0.001-300 thirty 3 30-160 10 7 inter feron water 10 62 0.001-200 twenty 5 30-180 10 8 inter feron water fifty 70 0.001-300 thirty 5 30-200 10 9 inter feron water 120 70 0.001-300 thirty 3 30-160 10

It can be seen from the examples given that, using the proposed device, by simple exposure to organic matter of natural origin, it is possible to conduct experiments on protein solutions, as a result of which we observed electrical vibrations, the maximum amplitude of which was 30 mV, and the frequency ranged from 1 kHz to 300 MHz. During the experiments, observation of the interference of the electromagnetic background and its measurement were also carried out. It was found that the generated signals are presented in table 1, are not the result of inducing man-made character and their source are protein solutions. The experimentally established signals of protein solutions are also not a consequence of chemical reactions occurring in these solutions, because model solutions do not consist of chemically active substances, but are only multicomponent systems (see Chemistry Handbook. Moscow. 1963, T. T. Berezov, B. F. Korovkin Biological Chemistry, Moscow, 1983; Biochemistry. Moscow. 1964). Those. experiments using the proposed device are clean.

The above experiments made it possible to notice the occurrence in protein solutions under the influence of an alternating magnetic field of electrical vibrations. This gives rise to additional information on macromolecules.

Thus, the inventive device allows you to conduct experiments in an alternating magnetic field over such objects as protein solutions. These experiments allow a deeper study of the properties of protein objects and open up new possibilities for their use.

Claims (3)

1. A device for conducting experiments on protein solutions in an alternating magnetic field, including a container of diamagnetic material, where a protein solution based on substances having hydrogen bonds is placed, two electrodes are located in the vessel, connected to an oscilloscope, and next to it to form a magnetic field an inductor connected to the generator is installed. 2. The device according to claim 1, characterized in that their aqueous or aqueous-alcoholic solutions are used as protein solutions. 3. The device according to claim 1, characterized in that plastic or glass can be used as the diamagnetic material of the container.