RU2542485C1 - Tongue worm repellent composition - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: what is presented is a tongue worm repellent composition containing essences of Cymbopogon citrates and Xanthoxylum armatum, and vanillin. The ratio of said essences of Cymbopogon citratus:Xanthoxylum armatum:vanillin makes 1:3:1, 1:1:3 or 8.5:8.5:3 (vl / vl / wt).

EFFECT: improving the tongue worm repellent action and increasing the length of the repellent action.

2 dwg, 6 tbl

Description

Technical field

The present invention relates to a repellent composition for repelling blood-sucking arthropods and, more particularly, to a repellent composition for repelling blood-sucking arthropods containing essential oils and vanillin, and thus, the repellent effect on blood-sucking arthropods such as mosquitoes and the like is improved. , and the duration of repellent action increases.

State of the art

Blood-sucking arthropods include various insects, such as mosquitoes, ticks, lighters, tsetse flies, horseflies, bed bugs, and the like. In particular, mosquitoes, which are the most dangerous blood-borne arthropods, are the main carriers of dengue, yellow fever, encephalitis and malaria, including West Nile virus, and infect between 0.3 and 0.5 billion people or more each year. Among the diseases described above, annual mortality due to malaria is 1 million or more of the total number affected (see Rozendaal, JA 1997. Vector control: methods for use by individuals and communities. WHO. Geneva, Switzerland: http: // www. who.int/water_sanitation_health/resources/vectorcontrol/en/).

Thus, various repellents have been developed that act on blood-sucking arthropods.

Such repellents are known as the most effective anti-infective agents for humans and livestock (see Curtis, CF, JD Lives, LU Baolib, and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In C. F. Curtis [editor], Appropriate technology in vector control, CRC Press, Boca Raton, Florida; Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and increasingly regulated world. Annu. Rev. Entomol 51: pp. 45-66).

However, the most widely used repellent, DEET (N, N-diethyl-3-methylbenzamide), despite its effectiveness, is known to have various negative effects, such as unpleasant odor, damage to plastic or rubber, suppression of the central nervous system, skin inflammation, DNA damage in mammals and the like. (see Katz, TM, JH Miller and AA Hebert. 2008. Insect repellents: historical perspectives and new developments. J. Am. Acad. Dermatol. 58: 865-871; Knowles, C. O. 1991. Miscellaneous pesticides, pp. .1471-1526. In JB Hayes, Jr. and ER Laws, Jr. [eds.], Handbook of pesticide toxicology, Academic Press, San Diego, California). Therefore, there is a need to develop safer repellents to prevent the attack of blood-sucking living organisms.

Meanwhile, vegetable essential oils have recently attracted attention as alternative resources for use in developing natural repellents that repel blood-sucking arthropods (see Ahn, YJ, SI Kim, HK Kim, and JH Tak. 2006. Naturally occurring house dust mites control agents : development and commercialization, pp. 269-289. In M. Rai and M. C. Carpinella [eds.], Naturally occurring bioactive compounds. Elsevier, London, United Kingdom .; Curtis, C. F., JD Lives, LU Baolib , and A. Renz. 1990. Natural and synthetic repellents, pp. 75-92. In CF Curtis [ed.], Appropriate technology in vector control. CRC Press, Boca Raton, FL .; Das, NG, I. Baruah, PK Talukdar, and S. C Das. 2003. Evaluation of botanicals as repellents against mosquitoes. J. Vector Borne Dis. 40: 49-53 .; Rozend aal, J. A. 1997. Vector control: methods for use by individuals and communities, World Health Organization, Geneva, Switzerland). Such essential oils are advantageous in that they contain various biologically active substances and do not adversely affect the environment and living organisms and are relatively inexpensive compared to plant extracts.

SUMMARY OF THE INVENTION

Technical problem

The present invention is intended to solve the problem, is a repellent composition for repelling blood-sucking arthropods, which solves the problems of existing synthetic repellents, is safer, improves the repellent effect against blood-sucking arthropods and increases the duration of repellent action.

Another objective of the present invention is the provision of a carrier comprising a repellent composition, and a device for distributing a repellent composition.

Technical solution

The purpose of the present invention can be achieved by providing a repellent composition for repelling blood-sucking arthropods containing essential oils and vanillin.

In another aspect of the present invention described herein, the porous carrier comprises the repellent composition described above.

In another aspect of the present invention described herein, an apparatus for distributing a repellent composition is provided.

Beneficial effects

According to the present invention, a repellent composition for repelling blood-sucking arthropods, a carrier including such a composition, and a repellent dispensing device improve repellent action against blood-sucking arthropods and increase the duration of repellent action. In addition, the repellent composition is safer than existing repellents.

Description of figures

1 is a view illustrating a test cell used in the cell test method of the present invention, in which the test cell includes a steel frame, a material processing section 1 and a release section 3 that are located on opposite sides of the test cell and are each coated polyester mesh, and untreated section 2 located in the Central part of the test cage and surrounded by an acrylic plate, and the holes in the polyester mesh are presented only with the purpose of illustration and therefore not proportional to the drawing, but the holes are too small for mosquitoes to penetrate through them; and

figure 2 illustrates a module comprising a ball-type repellent obtained according to the present invention.

Best option exercise

The repellent composition for repelling blood-sucking arthropods according to the present invention contains essential oils and vanillin.

The blood-sucking arthropods against which the repellent composition of the present invention can be applied include all living organisms that suck blood or body fluids through the skin of mammals or birds. Examples of blood-sucking arthropods include, but are not limited to, mosquitoes, blood-sucking flies, such as flies, tsetse flies, horseflies, etc., bugs, ploshchits and aphids.

The essential oil as used herein may be any, without limitation, essential oil known as a repellent for blood-sucking arthropods. Preferably, the essential oil can be obtained from Cinnamomum cassia, Rosmarinus officinalis, Eucaliptus citriodora, Cymbopogon cilratus, Xanthoxylum armatum, Cymbopogon nardus and the like. Essential oil can be obtained from the above plants using a standard solvent, for example, water, ethyl alcohol, acetone, methanol, propanol, ether, hexane, ethyl acetate, chloroform, or a mixture thereof, using one of the methods such as enthlerage, infusion, hot water extraction method (electromagnetic method), enzymatic extraction, percolation, supercritical fluid extraction, etc. More preferably, the essential oil can be obtained by steam distillation. In addition, the essential oil can be partially obtained from a plant, for example, roots, stems, leaves, wood pulp, flowers, fruits, seeds and / or peels, or can be obtained from a whole plant.

Vanillin, as used herein, refers to one of the aromatic aldehydes with vanilla flavor, i.e. 4-hydroxy-3-methoxybenzaldehyde. Any form of vanillin can be used according to any method known in the art. Commercially available vanillin may also be used. At the same time that various substances known as repellents for blood-sucking arthropods, vanillin was never ever known as a repellent for insects.

The repellent composition for repelling blood-sucking arthropods according to the present invention contains vanillin and one essential oil selected from the group consisting of Cinnamomum cassia, Rosmarinus offlcinalis, Eucaliptus citriodora, Cymbopogon citratus, Xanthoxylum armatum and Cymbopogon nardus. Moreover, the ratio in the composition of the essential oil to vanillin, in particular, is not limited, and their mass ratio can be from 1: 3 to 3: 1, taking into account the solubility in the solvent and mutual miscibility.

In addition, the repellent composition for repelling the blood-sucking arthropods of the present invention may include the two types of essential oils and vanillin described above. Moreover, the ratio in the composition of one of the essential oils to the other and vanillin, in particular, is not limited, and the ratio in the composition of one of the essential oils to the other and vanillin can be in the range 1-3: 1-3: 1-3 (vol / vol./mass.), more preferably, the range of 1: 1: 1, 3: 1: 1, 1: 3: 1, 1: 1: 3 or 8.5: 8.5: 3, taking into account the solubility in the solvent mutual miscibility and maximizing repellent action.

The repellent composition for repelling blood-sucking arthropods according to the present invention may further comprise an appropriate additive, excipient or carrier when formulated in the composition depending on the environment of the intended use or application. Examples of compositions include, but are not limited to, emulsions, emulsifiers, wettable powders, water soluble powders, aerosols, atomizable agents, suspensions, ultra-small volume (ULV) compositions, fragrant waters, granules, sprayable powders, powders, fine granules, capsules, ointments, creams, balls, tablets, extracts, liquid extracts, lotions and plasters. Preferably, the repellent composition may be formulated in the form of a cream, sprayable agent or aerosol.

In addition, the repellent composition for repelling the blood-sucking arthropods of the present invention can be applied to a porous carrier for sustained release. Any form of porous support may be used, for example, charcoal, silica gel, diatomaceous earth, and the like. The porous carrier includes a gel formed in such a way that it contains micropores as a result of the formation of bonds between the monomers constituting the polymer or viscopearl (trademark, manufactured by Rengo, Tokyo, Lacquer), and the compression of the polymer or polymer fiber. Viscopearl has excellent porosity and is thus suitable for use in the repellent composition of the present invention for gradual release.

Examples of applications for the repellent composition according to the present invention include, but are not limited to, direct scattering or spraying in places where blood-sucking arthropods mainly appear, direct application to the skin of mammals, including humans, livestock or birds, and application to household items, such as clothing, bags, headbands, tents, etc., or indoor use.

When used in places where blood-sucking arthropods are mainly common, the repellent composition can be directly scattered or sprayed in the air. In another embodiment of the present invention, for example, when applied to a specific space, such as a building, a porous carrier can be installed in the room continuously releasing the repellent composition.

When directly applied to the skin of mammals or birds, an appropriate amount of a repellent composition may be applied or sprayed onto their skin. In this case, the repellent composition should not cause skin irritation or, in case of irritation, it can be used in very small quantities that do not cause skin problems.

In addition, when applied to household items, capsules with a repellent composition on a substrate can be obtained, and the finished capsules can be directly interfered with the fabrics, dressings or plastics used to make household items, and then processed so that the repellent composition contained in the capsule , was released into the air during fracture of the capsule as a result of friction to manifest repellent properties, or was slowly released into the air in a directly mixed state. In one embodiment of the present invention, a porous carrier may be used that contains the repellent composition of the present invention. In this case, the device for the gradual release of the repellent composition may include an indicator indicating the consumption of the repellent composition using a method based, for example, on a color change when the repellent composition, which is the active ingredient, has completely disappeared.

The repellent composition for repelling blood-sucking arthropods according to the present invention can also be applied to household appliances such as an air purifier, an element of a ventilation system, an air conditioner, and the like. Their application to such household appliances can be accordingly modified by traditional methods. When applied to an air conditioner, an element of a ventilation system, an air conditioner, and the like that conduct air convection in a home, a repellent composition can be released into the purified air and therefore their application to such household appliances is particularly useful.

Hereinafter, specific examples will be described to understand the present invention. However, these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

An embodiment of the present invention

Example

1. Getting raw materials

Cinnamomum cassia, Rosmarinus offwinalis, Eucaliptus citriodora, Cymbopogon citratus, Cymbopogon nardus, and vanillin were purchased from Berje, Bloomfield, NJ, and Xanthoxylum armatum from Seema International, Delhi, India. In addition, DEET was purchased from Sigma-Aldrich (St. Louis, Missouri).

Each of the essential oils obtained from the plants described above and vanillin were dissolved in ethanol to obtain a composition. Each vegetable essential oil and vanillin were mixed in a ratio of 5%: 5% (vol./mass.) And 5%: 15% (vol./mass.), And each mixture was dissolved in ethanol to obtain 100 μl of each final composition.

Meanwhile, 5% (by volume) and 15% (by volume) solutions of ethanol containing DEET instead of essential oil and vanillin were obtained as controls.

In addition, to obtain compositions containing two types of essential oils and vanillin, two different types of essential oil were selected from Eucaliptus citriodora, Cymbopogon citratus and Xanthoxylum armatum and dissolved in ethanol with vanillin to obtain 100 μl of each composition. The ratios in the mixture of two types of essential oil to vanilla in each composition were 1: 1: 1, 3: 1: 1, 1: 3: 1 and 1: 1: 3, and these ratios in the mixture corresponded to 5%: 5%: 5 %, 15%: 5%: 5%, 5%: 15%: 5% and 5%: 5%: 15% (vol./vol./mass.), Respectively. In addition, the ratio in the mixture of Cymbopogon nardus, Xanthoxylum armatum and vanillin was 8.5: 8.5: 3 (v / v / mass) and the ratio in the mixture of Eucaliptus citriodora, Xanthoxylum armatum and vanillin was 8.5: 8 5: 3 (v / v / mass).

2. Obtaining experimental insects

For use in the Ae mosquito imago test. Aegypti, successively grown indoors for several years without exposure to insecticides, were stimulated to multiply after suction of blood directly from the mouse, and they were supplied with 10% (w / v) aqueous sugar to make up for the deficiency in carbohydrates. Larvae Ae. aegypti was placed in a tray (24 × 35 × 5 cm) and fed 0.5 g of sterile artificial feed (feed powder for fry with a particle size of 40 mesh / yeast, 4: 1 wt./mass.). The larvae were kept at a temperature of 26 ± 2 ° C, relative humidity of 70 ± 5% and day: night mode 16: 8 hours.

3. Experiment

A. Skin Test Method

To assess the repellent properties of the composition of the present invention with respect to mosquito imago, a skin test was carried out by uniformly treating the back of the hands of the participants who wore rubber gloves, each of which had a 5 cm diameter hole through which the surface of the arm was exposed, with the compositions obtained on stages of obtaining the starting materials above. The participants in the experiment were healthy men aged 21 to 40 years. As a control, only 100 μl of ethyl alcohol was used.

5-8-day-old female imagoes aegypti (250-260 adults) were obtained in a cage (25 × 25 × 25 cm ³ ) for growing adult mosquitoes for 12-24 hours before the start of the experiment. The participant’s forearm was exposed as a control for 1 minute and when at least six female Ae adults. aegypti sat on their forearms or showed blood-sucking behavior, bite pressure was considered sufficient and a repellent test was performed. The forearms of the experimental participants were treated with compositions, exposed for 3 minutes with an interval of 30 minutes, and when at least two female imagoes Ae. aegypti continuously showed bloodsucking behavior, such as landing on the forearm for 2 seconds or longer, introducing a mosquito trunk into the skin, finding a position for a bite, etc., the experiment was stopped. The time interval between the initial moment of application of the composition and the moment in which at least two adults continuously sat on the treated area or continuously showed such blood-sucking behavior was considered the duration of complete protection (CPT).

CPT indicates the period of time between the treatment time and the start time of blood absorption, and for comparison between the average CPT values for each treatment, a general linear model (GLM) analysis was performed. For comparison between average CPT values, the Bonferoni test was used (P = 0.05).

The results are shown in tables 1 and 2 below. Table 1 shows the CPT values for each essential oil, vanillin and compositions of each essential oil and vanillin in relation to the imago Ae. Aegypti, and CPT values for Ae imago are also shown. aegypti, when using DEET alone. Table 2 shows the results of CPT for the compositions of two types of essential oils and vanillin in relation to imago Ae. aegypti.

Table 1 Average CPT (range, min) No vanillin With vanilla 5% fifteen% 5%: 5% 5%: 15% 15%: 5% 15%: 15% Cinnamomum cassia 75 (60-90) - * 135 (120-150) 157.5 (120-180) - * - * Rosmarinus offwinalis 0 (0) 60 (60) 52.5 (30-60) 112.5 (60-150) 60 (30-90) 135 (120-150) Eucaliptus citriodora 22.5 (0-30) 52.5 (30-60) 60 (60) 67.5 (30-120) 75 (60-90) 90 (60-120) Cymbopogon citratus 30 (30) 37.5 (30-60) 105 (90-120) 97.5 (60-120) 105 (90-120) 150 (150) Xanthoxylum armatum 30 (30) 75 (60-90) 60 (60) 90 (60-120) 75 (60-90) 120 (90-150) Cymbopogon nardus 25 (25) 70 (60-80) 55 (55) 85 (50-120) 70 (60-80) 105 (85-125) Vanillin 15 (0-30) 37.5 (0-60) DEET 127.5 (120-150) 247.5 (240-270) * Experimental values have not been obtained due to severe skin irritation.

As can be seen from table 1 above, the group treated with only 5% Cinnamomum cassia showed a CPT of 75 minutes, the groups treated with 5% Cymbopogon citratus, Xanthoxylum armatum, Eucaliptus citriodora and vanillin, respectively, showed CPT values of 30 minutes. 30 minutes, 22.5 minutes and 15 minutes and the group treated with 5% Rosmarinus offwinalis did not show any effect. Contrary to the results, the group treated with 5% DEET showed the strongest repellent effect, i.e. CPT was 127.5 minutes and CPT increased to 247.5 minutes with 15% DEET. On the other hand, as can be seen from table 1 above, the compositions containing essential oil and vanillin had an enhanced repellent effect due to the synergistic effect between them, compared with the groups that were treated with each substance separately. This increased repellent effect was confirmed by the fact that when vanillin was mixed with each essential oil in the amount of 5% and 15%, CPT increased sharply (F = 71.75, P <0.0001). The results confirm that two factors, i.e. the concentration of the essential oil and the addition of vanillin to it are significantly significant (F = 5.61, P <0.0001).

In parallel, two types of essential oil and vanillin were mixed to obtain repellent compositions according to the present invention, and then the same experiment was carried out. The experimental results are shown in table 2 below.

Group A is Eucaliptus citriodora: Cymbopogon citratus: vanillin

Group B means Eucaliptus citriodora: Xanthoxylum armatum: vanillin

Group C represents Cymbopogon citratus: Xanthoxylum armatum: vanillin

Group D means Cymbopogon nardus: Xanthoxylum armatum: vanillin

As can be seen from table 2, compositions containing two types of essential oil and vanillin, showed a significant increase in repellent action (CPT) compared with compositions containing each essential oil and vanillin. The results confirm that this action results from a synergistic effect between them, which is greater than an increase in CPT as a result of simply mixing each essential oil with vanilla. Thus, in group A, which includes the composition components in a 1: 1: 1 ratio, the arithmetic CPT for group A is only 67.5 minutes, which is obtained by adding individual CPTs for Cymbopogon citratus, Eucaliptus citriodora and vanillin i.e. 30 minutes, 22.5 minutes and 15 minutes, respectively. However, as shown in Table 2, it can be verified that the experimental CPT for group A is 105 minutes, which is approximately two times greater than the expected CPT. Thus, the results confirm that the repellent compositions for the blood-sucking arthropods of the present invention, containing two types of essential oil and vanillin, have a synergistic effect.

B. Test Cell Method

The present test was carried out to check whether mosquitoes penetrate the treatment section through a dense cardboard plate (module window) located in the center of the test system in the form of a wall and containing a 10 cm diameter hole in its central part. In addition, the test was carried out to check the average repellent effect of the ball composition over time.

In the test, the composition of the present invention was applied in the form of filter paper, treated with 5 μl of the composition, or formulated into bead formulations. The ball composition was obtained by impregnating porous cellulose beads (Viscopearl®, size: 3.8-4.2 mm, manufactured by Rengo Co. Ltd, Tokyo, Lacquer) with the repellent composition of the present invention. The amount of composition in 1 g of the finished composition with viscopearl beads was 0.465 g and the weight of viscopearl was approximately 0.535 g. The ratios of the components in each composition used are shown in table 3 below.

A cell test required 100 5-10-day Ae adults. Aegypti, which were obtained 2 hours before the start of the cell test. A cell for cell testing is illustrated in FIG. The cell included a treated material section 1, an untreated section 2 and a release section 3, in which a treated material section 1 and a release section 3 were coated with a polyester mesh and an untreated section 2 was surrounded by an acrylic plate. Section 2 without treatment was installed in the center of the cage with a dense cardboard plate with a hole with a diameter of 10 cm. The mouse as an attraction for mosquitoes was placed in section 1 with the treated material 10 minutes before the start of the experiment so that the mouse was fixedly fixed on an iron mesh, and a filter the paper treated with the repellent composition of the present invention and the repellent composition in the form of balls were placed in section 1 with the treated material. The filter paper was treated with a repellent composition, naturally dried for 1 minute, hung from the top of section 1 with treated material on a thread, and the substance was allowed to volatilize for 3 minutes. After that received the female imago Ae. aegypti was launched into release section 3. In the test, the natural direction of movement of the mosquitoes was from release section 3 to section 1 with the treated material.

The average repellent ratio, indicating the extent to which the movement of mosquitoes into section 1 with the treated material is limited, was calculated using the following equation:

The average repellent ratio (%) = {(30-number of mosquitoes introduced into the section with the processed material) / 30} × 100

The results are shown in table 3 below.

A = Eucaliptus citriodora

B = Cymbopogon citratus

C = Xanlhoxylum armatum

D = Vanillin

As can be confirmed from table 3 above, from an assessment of how the penetration of mosquitoes into the section with the processed material was limited to filter paper directly processed by each of the compositions obtained in accordance with the ratios in the compositions shown in table 3, all compositions except which used filter paper treated with a composition obtained by mixing Eucaliptus citriodora, Xanthoxylum armatum and vanillin in a ratio of 3: 1: 1, showed a repellent ratio for mosquitoes avnoe 80% or more even after 120 minutes after the experiment.

In addition, in a similar way, when 1 g of ball compositions obtained by soaking Viscopearl® (size: 3.8-4.2 mm, manufactured by Rengo Co. Ltd, Tokyo, Lacquer) of each of the compositions obtained according to the ratios in the compositions were used shown in table 3, almost all ball compositions showed a repellent ratio of 80% or more for mosquitoes. Thus, from the results, it can be ascertained that when the active ingredients of the composition of the present invention are dispersed in space at a certain concentration or higher, mosquitoes recognize the repellent composition and do not wish to enter the space in which the aroma of the repellent composition is common.

C. Chamber test

A chamber test was performed to test the repellent effect of the composition of the present invention in a large space. The chamber (size: 1.2 × 1.2 × 1.2 m) used in the test had a steel frame and was covered with an acrylic film, in the front lower part of which there was a square hole (20 × 20 cm). The square hole was covered with a black cardboard plate with 10 holes (hole size: 1.5 × 1.5 cm). A mosquito release cell (size: 25 × 25 × 25 cm) was attached to the square hole. Another collection cell was placed in a chamber. The release cell and the collection cell were joined together by a cardboard plate and both were coated with a polyester mesh. A fan (model name: EFB-2-3, manufactured by Hanil, Korea) was turned on to accelerate the diffusion of the ingredients contained in the composition. A volunteer man came into the chamber as an attractor for mosquitoes. As a control, the number of mosquitoes that moved into the chamber for 2 hours when the volunteer was in the chamber without a repellent composition was counted. A module containing a ball-type repellent, which will be described below, was used as a repellent. The attractor material was processed for 30 minutes before the test and the repellent was processed 1 hour before the test. The experiments were repeated and the results obtained by statistical processing.

The ball type repellent was the same as that used in the cell test method. A ball type insect repellent composition was prepared by mixing Cymbopogon citratus, Xanthoxylum armatum and vanillin in a ratio of 8.5: 8.5: 3 (v / v / mass) in ethyl alcohol as a solvent.

In addition, for the present experiment, a module was created with a shape that is illustrated in FIG. The module had 10 openings with opening or closing control, and each hole contained the same amount of ball-type repellent, so the module contained a total of about 10-11 g of ball-type repellent, and approximately ball-type repellent was impregnated with 4.7-5.4 g composition.

Two sets of modules, 10 openings in which were fully open, were placed in a chamber and the number of 5-10-day-old aegypti female adults was 120. The number of female mosquito adults that entered the chamber was counted 5, 10, 20, and 30 minutes after turning on the fan . The repellent effect was calculated as a repellent ratio for each point in time, which is the ratio of the number of mosquitoes entering the chamber without using a repellent to the number of mosquitoes entering the chamber when applying the composition of the present invention, and the average of the obtained repellent relations was calculated and accepted for the total repellent ratio (%).

The calculation of the repellent relationships for each particular point in time was repeated three times to obtain an average repellent relationship. The calculation results are shown in table 4 below.

1. Cymbopogon nardus: Xanthoxylum armatum: vanillin = 8.5: 8.5: 3 (v / v / mass)

2. Cymbopogon citratus: Xanthoxylum armatum: vanillin = 8.5: 8.5: 3 (v / v / mass)

3. Cymbopogon nardus: Xanthoxylum armatum: vanillin = 1: 1: 1 (v / v / mass)

4. Cymbopogon citratus: Xanthoxylum armatum: vanillin = 1: 1: 1 (v / v / mass)

The results shown in table 4 above confirm that the mosquito repellent of the present invention has an excellent repellent effect even in large spaces and, in particular, a repellent composition containing Cymbopogon citratus: Xanthoxylum armatum: vanillin in the ratio of 8.5: 8.5: 3 (v / v / mass) showed excellent repellent effect.

In parallel, to assess the repellent ratio and duration of action of a composition containing Cymbopogon citratus: Xanthoxylum armatum: vanillin in a ratio of 8.5: 8.5: 3 (v / v / mass), the change in repellent action over time was observed by chamber test. The results are shown in table 5 below.

As can be seen from table 5 above, it can be verified that the composition showed a total average repellent ratio of 83% or more and had a repellent ratio of 70% or more after at least 60 minutes.

D. Acceleration Test

The duration of the repellent action of the repellent composition for repelling the blood-sucking arthropods of the present invention was measured using an acceleration test. The repellent composition was applied on average for approximately 8 hours per day. Although the number of holes in the chamber wall involved in the chamber test through which mosquitoes can move was 10, the number of holes in the wall inside the chamber in the acceleration test was 40, and the test was continuously performed for 24 hours. To assess whether the repellent effect of the blood-sucking arthropod repellent composition of the present invention lasts for 90 days, the repellent effect was measured at specific points in time after the test was performed for 8 days under the conditions described above.

The acceleration test was carried out in the same chamber that was used in the chamber test and in the same way as in the chamber test, except that the number of holes was 40. The control group was treated with ethyl alcohol alone and the group which was treated with a composition obtained by mixing Cymbopogon citratus: Xanthoxylum armatum: vanillin in a ratio of 8.5: 8.5: 3 (v / v / mass) in ethyl alcohol as a solvent, was used as an experimental group. The results are shown in table 6 below.

From the results shown in table 6 above, it can be verified that when the ball-type composition obtained by impregnating the balls with the repellent composition of the present invention was treated, the repellent effect of the ball repellent was maintained on average by 60% or more for 90 days or longer.

Claims (1)

A repellent composition for repelling blood-sucking arthropods, containing essential oils obtained from plants of Cymbopogon citratus, Xanthoxylum armatum and vanillin, in which the ratio of these essential oils of Cymbopogon citratus: Xanthoxylum armatum: vanillin is 1: 3: 1, 1: 1: 3 or 8, 5: 8.5: 3 (v / v / mass).

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