RU23683U1 - KIT FOR DETERMINATION OF DRUG, PSYCHOTROPIC AND STRONGLY ACTING SUBSTANCES OF VEGETABLE AND SYNTHETIC ORIGIN - Google Patents

Description

Set for determination of narcotic, psychotronic and

potent substances of plant and

synthetic origin.

The technical solution relates to the field of analytical chemistry, namely to the field of determination of narcotic, psychotropic and potent substances of plant and synthetic origin, and can be used to determine these substances in off-laboratory conditions, namely, customs units, operational units of the Ministry of Internal Affairs, as well as units of the Ministry of Internal Affairs I work offline.

Known (US patent 4992296) is a method for determining narcotic substances by changing the color of the indicator substance previously applied to the filter paper, after bringing the sample containing the narcotic substance into contact with the filter paper.

A disadvantage of the known technical solution should be recognized as a limited number of defined narcotic substances, as well as their weak distinguishability and, therefore, low accuracy of determination.

There is a known (RU, patent 2053771) method for the determination of narcotic substances, namely morphine, promorphin, caffeine, dionine, hydrogyodone, marijuana, cocaine, codeine, heroin, amphetamine. According to a known method, a urine sample is applied to indicator paper, which is a flexible filter impregnated with solutions of oxide of ferric chloride, potassium ferroschanide.

MnK: 7G01N21 / 78

./;

, u}

potassium permanganate, dinitrophenylhydrazone, nitric acid and sulfuric acid. The indicator with the applied sample is heated and the presence of these drugs is determined by the color change of the indicator.

A disadvantage of the known technical solution should be recognized as a limited number of defined narcotic substances, as well as their weak distinguishability and, therefore, low accuracy of determination.

The closest analogue of the proposed technical solution can be recognized as a method for determining narcotic, psychotropic and potent; their substances of plant and synthetic origin (RU, patent 2138044). According to a known method, the sample is reacted with a reagent to obtain a colored medium, the color of which determines the presence of the drug, moreover, the reagents are Marky's reagent and an aqueous buffer solution with a pH of 2.0 - 7.8 containing chloride, sulfate or nitrate of bivalent cobalt , potassium, sodium or ammonium thiocyanates, as well as indicator and chloroform. In addition, Mandelin, Erlich, Mecca and other solutions can be used as reagents. All reagents and solutions used are placed in separate vials closed with stoppers, and the stoppers are simultaneously samplers and dispensers.

A disadvantage of the known technical solution should recognize the duration of the determination, due to the design of the bottles and plugs, and also due to the same reason, the lack of accuracy of determination.

The technical problem solved by the proposed set is to provide the ability to quickly and accurately determine narcotic, psychotropic and potent substances

of plant and synthetic origin in off-laboratory conditions.

The technical result obtained by implementing the kit is to increase the efficiency and reliability of the determination of these substances, as well as the composition of medicines of unknown composition.

To achieve the specified technical result, it is proposed to use a kit containing a large test pencil case,

including at least 7 ampoules containing reagents for the determination of synthetic drugs (ephedrine, pseudoephedrine, methaqualone, amphetamines, methadone, tramadol, mescaline, heroin, codeine, aprofen, cyclodol, diphenhydramine, promedol, phencyclidine, amisil, LSD, morphine, LSD, morphine cocaine, barbiturates, etc.), and a small testpenal, including at least 2 ampoules containing reagents for the determination of drugs of plant origin (opiates, poppy straw, ephedra grass, hemp and its processed products), and at least least nine the casing of samplers, which is a hollow polyethylene glass, preferably, in the lower part of which a cylindrical protrusion is made on the outside, on which a thin layer of non-drying sticky polymer is applied, each ampoule made in the form of a tube sealed on both sides, inside which reagent solutions are placed, while each ampoule has a ring notch for opening the ampoule, a polyethylene sleeve made in the form of a short tube is tightly put on each ampoule, and the sleeve is put on the ampoule so that The annular notch of the ampoule is located inside the coupling.

The device may further comprise a container with a catalyst, preferably alumina. The device may also contain a flat liner, on the surface of which colored stripes are applied, corresponding to the color scheme, which

can be obtained in the reaction volume in the presence of one of the determined narcotic substances in the sample, the color code of the narcotic substance corresponding to each of the narcotic substances determined using the proposed device, as well as a table for filling out the color code of the analyte. The term “color code of a narcotic substance within the framework of the present invention characterizes a set of numbers or symbols of color bars corresponding to the colors of solutions in each of the ampoules, after the interaction of the reagent in the ampoule with the defined narcotic substance. Ampoules of a large testpenal are filled with at least the following reagents: Marki reagent, a reagent containing from 1% to 2.5% CoCb, Co (MOH) 2 or CoSO4, from 0.01% to 3% (diethylamino) phenylazole benzenesulfonic acid in an aqueous buffer with a pH of 2.0 to 7.8 and chloroform, Mandelin’s reagent, a reagent containing 1% to 2.5% CoCb, Co (1Oz) 2 or CoSO4, 1% to 3.2% KSCN, NaSCN or NH4SCN in an aqueous buffer with pH from 2.0 to 7.8 and chloroform, Mecca reagent, a reagent containing from 0.2% to 5% CuSO4 solution in 1-5% piperidine solution in water, Erlich reagent. Ampoules of a small test case are filled with at least the following reagents: a reagent containing from 0.01% to 2% aqueous solution of 9 (2-butoxycarbonylphenyl) -6-diethylamino) ZN-xanthen-3-iden diethylammonium chloride and hexane, and a reagent containing from 2.5% to 5.5% CoC, Co (S03) 2 or CoSO4, from 10% to 20% KSCN, NaSCN or NH4SCN, from 0.01% to 3% (diethylamino) phenylazole benzenesulfonic acid in aqueous buffer with a pH of from 2.0 to 7.8 and chloroform.

The graphic pencil case shows the view of the large and small pencil cases, with the following notation taken: ampoule 1, plug 2, sleeve 3, large test - pencil case 4, small test - pencil case 5.

4

When implementing the device, the ampoules of a large pencil case and / or small pencil case break off, so that the break remains inside the coupling, each sampler with an adhesive layer is filled with a sample of the analyte, the sampler is inserted into the coupling on an opened ampoule, and the sample is brought into contact with the reagent placed in the ampoule , and after no less than 6 seconds, compare the color with the set of color strips applied under each ampoule. The number or symbol of the color bar, the color of which corresponds to the color of the reagent interacting with

analysis of 1 substance. If the color of the solution in the ampoule does not coincide with any of the colors in the color bars, then a dash is placed in a free cell, which is also an element of the code. After filling in the free cells on the code table, the numbers or symbols of the recorded code are compared with the numbers or symbols in the corresponding cells of the table on the back of the insert. The coincidence of the numbers or symbols of the recorded color code with one of the sets of numbers or symbols in the cells on the back of the insert indicates the probable presence in the test sample of a substance whose code matches. If the reagent in the ampoule has two layers, then the color of the solution is determined by the color of the previously indicated layer. If there is a container with a neutralizer, it is advisable to place the samplers and opened ampoules after the end of work in the indicated container. It is desirable to grind a sample of plant material before determination.

In a preferred embodiment, the device is packaged in a transparent 10x15 cm plastic bag with a flexible fastener.

The packaging plastic bag contains: 1. A large test case (7 ampoules) for the determination of ephedrine, pseudoephedrine, methaqualone, amphetamines, methadone, tramadol.

iccf.sesalin, heroin, codeine, aprofen, cyclodol, diphenhydramine, promedol, phencyclidine, amisil, LSD, morphine, cocaine, barbiturates, etc.

2. Small test case (2 ampoules) for the determination of opiates, poppy straw, ephedra grass, hemp and its processed products. 3. A transparent plastic bag with a flexible fastener of 7x12 cm in size with a neutralizing agent - aluminum oxide (10 g). 4. Instructions for using the kit.

The large test case is made in the form of a vertical case made of a transparent polymeric material containing at least 7 large cells for ampoules and 7 small cells for samplers, with small cells located above the large cells. A pocket is also made from the back of the pencil case over its entire length, also made of a transparent polymeric material into which a cardboard insert is placed.

Ampoules are made in the form of tubes 7 mm in diameter, sealed from the engine side, inside of which there are reagent solutions. Each ampoule at a distance of 1 cm from the upper part has an annular notch for opening the ampoule. Each ampoule is tightly fitted with a polyethylene coupling made in the form of a short tube 1 cm high. The coupling is designed to provide sealing of the opened ampoule. The sleeve is put on the ampoule so that the annular notch of the ampoule is located inside the sleeve, approximately in the middle of it. When the ampoule is opened, depressurization occurs along an annular notch, and after the broken part of the ampoule is removed, the upper part of the sleeve becomes open for introducing the analyzed sample into the reaction volume of the ampoule, while the lower part of the sleeve fits tightly around the neck of the opened ampoule.

The introduction of the analyzed powdery sample into the reaction volume and sealing of the opened ampoule is carried out using a cork, which simultaneously performs the function of a sampler. The cork is a hollow polyethylene cup 2 cm high, preferably in the lower part of which a cylindrical protrusion with a diameter of 2 mm and a length of 5 mm is made outside. A thin layer of non-drying sticky polymer is applied to the cylindrical protrusion. This design of the sampler allows you to select and add to the reaction volume a metered amount of a powder-like sample and at the same time reliably seal the opened ampoule due to the tight joint of the sampler with the inner surface of the coupling.

Ampoules are located in large cells of the pencil case, and samplers above the ampoules in small cells, and the protrusions of the samplers are directed upward from the ampoules.

The cardboard insert located in the pocket of the test pencil case is designed, firstly, to stiffen the pencil case and facilitate opening of the ampoules, and, secondly, to deposit information necessary for analysis on it. The insert can be moved down along the pencil case until it is completely removed.

On the side of the cardboard insert that faces the ampoules, opposite each ampoule, a column contains colored bars (color code) that correspond to the color scheme that can be obtained in the reaction volume if one of the narcotic substances in the sample is used to determine which the device is designed to be. Each color bar in a column has its own number or symbol. When carrying out the analysis, one should compare the crawl color of the reaction medium with the color of the color bars in the column and on the free field on the cardboard insert under each

ijgOi /

write an ampoule number or symbol color, which is closest to the color of the reaction medium. If the color of the reaction medium does not coincide with the given colors of the color bands, then a dash is put in the free field. Thus, after analysis, a code is obtained whose number of digits or symbols corresponds to the number of ampoules of a large pencil case.

A table is made on the back of the cardboard insert, in which the names of narcotic, psychotropic, and potent substances and the corresponding codes are given. The coincidence of the code in the table with the code obtained as a result of the analysis allows us to conclude that there may be a substance in the analyzed sample whose code is the same.

If the pencil case is made of a solid polymer material, for example, polystyrene, or its rigidity is sufficient to open the ampoules, then instead of a cardboard liner, a sheet of writing paper of the appropriate format can be used to apply the necessary information. In this case, the sheet is folded in half in the form of the Latin letter V and glued to the back wall of the pencil case with one part. During the analysis, the free part of the sheet is bent down.

Ampoules of a large test case are filled with at least the following reagents:

I. Reagent Marya.

P. A reagent containing from 1% to 2.5% CoC, Co (MOH) 2 or CoSO4, from 0.01% to 3% (diethylamino) phenylazo benzenesulfonic acid in an aqueous buffer with a pH from 2.0 to 7.8 and chloroform.

S. Reagent Mandeline.

IV. A reagent containing from 1% to 2.5% CoC, Co (KOz) 2 or CoSO4, 1% to 3.2% KSCN, NaSCN or NUjSCN in an aqueous buffer with a pH from 2.0 to 7.8 and chloroform.

V. Meyuse reagent.

VI. A reagent containing from 0.2% to 5% solution of CuSO4 in a 1-5% solution of piperidine in water.

Vn. Erlich reagent.

The small test pencil case has the same structural elements and with the same purpose as the large test pencil case. Unlike a large test case, a small test case is designed to use at least 2 ampoules, and the samplers used are made in the form of hollow polyethylene cups 2 cm high, preferably without cylindrical protrusions. In this case, the sample is taken inside the glass, which also provides a metered introduction of the sample into the reaction medium. This design of the sampler is due to the fact that the analyzed samples are usually products of plant origin, and they are used for analysis in the form of finely chopped particles.

Ampoules of a small test case are filled with at least the following reagents:

VIII. A reagent containing from 0.01% to 2% aqueous solution of 9- (2butoxycarbonylfesh) -6-diethylamino) 3H-xanthen-3-iden diethylammonium chloride and hexane.

IX. Reagent containing from 2.5% to 5.5% CoCb, Co (KOz) 2 or CoSO4, from 10% to 20% KSCN, NaSCN or NKtSCN, from 0.01% to 3% (diethylamino) -fenzo benzenesulfonic acids in aqueous buffer with a pH of from 2.0 to 7.8 and chloroform.

Since the composition of the reagents includes acids and organic solvents, after the end of the analysis, their disposal is desirable. As an absorbent and neutralizing agent, alumina is preferably used.

If there is a container with a neutralizer after analysis, the samplers are removed from the ampoules, and the pencil case is turned over with the open parts of the ampoules down, while simultaneously placing them in a bag with a neutralizer. Samplers are placed in the same package.

When using the proposed device in the preferred embodiment, it is necessary to take the test cases and the package with the converter out of the package and place them in front of you, and then open and place the package with the converter upright in front of you. Having taken a large test pencil case, place it in the left hand so that four fingers of the palm are on the back of the test on the bottom of the middle of the ampoules, and the thumb is on the front of the test. Holding the lower part of the ampoules with the thumb, successively break the upper parts of the ampoules from right to left, pressing on them with the thumb of the right hand. Ampoules should break inside the polyethylene sleeve. Broken off short ends of ampoules will interfere with a package with a converter. The cardboard insert is pushed down so that the upper edge of the color plate is 1-2 mm below the bottom of the ampoules. The free part of the transparent pencil case is bent so that the samplers located in the upper part are arranged with a cylindrical protrusion down. Starting from the right, the samplers are sequentially removed from the cells, and either the cylindrical protrusion with the sticky layer of each sampler is placed in a test object previously ground to a powder state, trying, if possible, to collect more powder, or fill the void. A small sample cup, and then insert the sampler into polyethylene sleeve on an ampoule. Samplers should fit snugly against the walls of the couplings. Samplers must be inserted into the ampoules opposite which they are located. After putting everyone

ic.

samplers in polyethylene ampoule couplings turn the testpenal upside down with the samplers down and hold it in this position for 1 minute, then turn the pencil case upside down and shake the pencil case 3-4 times.

When the sample is in contact with reagent I (Marki's reagent), its color does not change with barbiturates, cocaine, cocaine base, crack. Ephedrine, pseudoephedrine, amphetamine give a slight yellow tint to the reagent, amphetamines cause diphenhydramine, diphenhydramine, cyclodol, aprofen, red and orange stains cause promedol, tramadol, methadone and mescaline, violet and gray colors cause LSD, morphine, heroin, phenin codeine, amisil blue staining. The appearance of a yellow color of the chloroform layer in reagent II after interaction with the analyte indicates the presence of ephedrine, pseudoephedrine, methaqualone, amphetamines, methadone, tramadol, mescaline, heroin and codeine, a green color of aprofen, cyclodone, diphenhydramine, promedol, phencyclin and amyzine and barbiturates, LSD and morphine do not change the color of chloroform. A slightly yellow color of reagent III indicates the presence of ephedrine and pseudoephedrine in the sample, yellow or brown in color - methaqualone, diphenhydramine, methadone, amphetamines, mescaline, aprofen, cyclodone and amisine, green in color - LSD, feshchklidin and codeine, gray and violet color - , tramadol, morphine and heroin. The blue color of reagent IV indicates the presence of cocaine base. Cocaine hydrochloride and crack. The yellow color of reagent V (Mecca reagent) indicates the presence of methadone, mescaline and amisine, brown promedone, blue - morphine, heroin, codeine, green - phencyclidine, purple - thromadol. The blue color of reagent VI indicates the presence of barbiturates. The burgundy-violet color of reagent VII (Ehrlich reagent) indicates the presence of LSD. Red or

l ciicyj / 6

the brown color of the organic phase of reagent VIII indicates the presence of hemp and its processed products in the sample, and the green color of the organic phase of reagent IX indicates the presence of opium or poppy straws in the sample, yellow indicates the grass of ephedra, because of the absence of color they suggest the presence of hemp and / or its products processing.

After filling in the free cells on the color table, remove the cardboard liner from the pencil case. Then take out the samplers from the nozzles, slightly shaking them, and place them in a bag with a neutralizer. Holding the ampoules of the test case, place it in a package with a neutralizer with the open part of the ampoules down. Then close the package with the neutralizer. The numbers or symbols of the recorded code are compared with the numbers or symbols in the corresponding cells of the table on the back of the insert. The coincidence of the numbers or symbols of the recorded code with one of the sets of numbers or symbols in the cells on the back of the insert indicates the probable presence in the test sample of a substance whose code matches.

The analysis time of the sample using seven ampoules of a large testpenal is 2-5 minutes. The analysis time of plant sfia using two ampoules of a small test case is 10-15 minutes. The reliability of the determination is not less than 94% when the content in the analyzed sample is not less than 10% of the analyte.

12

Claims (7)

1. A kit for the determination of narcotic, psychotropic and potent substances of plant and synthetic origin, containing reagent bottles and plugs, the plugs being both samplers and dispensers, characterized in that it is made in the form of a kit containing a large test case, including at least seven vials made in the form of an ampoule containing reagents for the determination of synthetic drugs, and a small test case including at least two vials made in the form of an ampoule, with holding reagents for the determination of drugs of plant origin, and at least nine cork samplers, which are a hollow polyethylene glass, each ampoule made in the form of a tube sealed on both sides, inside which there are reagent solutions, each ampoule has an annular notch for opening the ampoule, a polyethylene sleeve made in the form of a short tube is tightly put on each ampoule, and the sleeve is put on the ampoule so that the annular notch of the ampoule is located inside three clutches.  2. The kit according to claim 1, characterized in that in the lower part of the hollow glass a cylindrical protrusion is made outside, on which a thin layer of non-drying sticky polymer is applied.  3. The kit according to claim 1, characterized in that it further comprises a container with a converter.  4. A kit according to claim 3, characterized in that aluminum oxide is used as a neutralizer.  5. The kit according to claim 1, characterized in that it further comprises a flat liner, on the surface of which are colored stripes corresponding to the color scheme, which can be obtained in the reaction volume in the presence of one of the determined narcotic substances in the sample, the color code of the narcotic substance, corresponding to each of the narcotic substances determined using the proposed device, as well as a table for filling out the color code of the analyte. 6. The kit according to claim 1, characterized in that the ampoules of the large test case are filled with at least the following reagents: Marki reagent, reagent containing from 1% to 2.5% CoCl ₂ , Co (NO ₃ ) ₂ or CoSO ₄ , from 0.01% to 3% of [4- [4-diethylamino) phenylazo] benzenesulfonic acid in an aqueous buffer with a pH of from 2.0 to 7.8 and chloroform, a Mandelin reagent, a reagent containing from 1% to 2 , 5% CoCl ₂ , Co (NO ₃ ) ₂ or CoSO ₄ , 1% to 3.2% KSCN, NaSCN or NH ₄ SCN in an aqueous buffer with a pH of 2.0 to 7.8 and chloroform, Mecca reagent, a reagent containing from 0.2% to 5% solution of CuSO ₄ in a 1-5% solution of piperidine in water, Erlich's reagent. 7. The kit according to claim 1, characterized in that the ampoules of the small test case are filled with at least the following reagents: a reagent containing from 0.01% to 2% aqueous solution of [9- (2-butoxycarbonylphenyl) -6-diethylamino ) 3H-xanthen-3-idene] diethylammonium chloride and hexane, and a reagent containing from 2.5% to 5.5% CoCl ₂ , Co (NO ₃ ) ₂ or CoSO ₄ , from 10% to 20% KSCN, NaSCN or NH ₄ SCN, from 0.01% to 3% of [4- [4- (diethylamino) phenylazo] benzenesulfonic acid in an aqueous buffer with a pH of from 2.0 to 7.8 and chloroform.