RU2017117030A - Получение тиндализированных, интактных и иммунологически активных клеток lactobacillus rhamnosus GG и способ их качественного и количественного определения - Google Patents
Получение тиндализированных, интактных и иммунологически активных клеток lactobacillus rhamnosus GG и способ их качественного и количественного определения Download PDFInfo
- Publication number
- RU2017117030A RU2017117030A RU2017117030A RU2017117030A RU2017117030A RU 2017117030 A RU2017117030 A RU 2017117030A RU 2017117030 A RU2017117030 A RU 2017117030A RU 2017117030 A RU2017117030 A RU 2017117030A RU 2017117030 A RU2017117030 A RU 2017117030A
- Authority
- RU
- Russia
- Prior art keywords
- cells
- lactobacillus rhamnosus
- atcc
- bacterial cells
- thindalized
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1488—Methods for deciding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (16)
1. Способ подсчета количества мертвых клеток, имеющих интактную клеточную мембрану, в образце тиндализированных бактериальных клеток Lactobacillus rhamnosus GG (АТСС 53103), включающий:
- получение посредством серийных разведений образца, содержащего тиндализированные бактериальные клетки Lactobacillus rhamnosus GG (АТСС 53103) в концентрации от 105 до 107 клеток/мл;
- добавление к указанному образцу первого реагента, представляющего собой тиазол оранжевый, и второго реагента, представляющего собой пропидий йодид, с получением раствора;
- добавление к указанному раствору суспензии флуоресцентных микросфер в азиде натрия с получением тестируемого образца;
- осуществление в указанном тестируемом образце общего подсчета клеток, включая живые клетки и мертвые клетки, и подсчета только мертвых клеток посредством проточной цитофлюорометрии;
- подсчет мертвых клеток, присутствующих в тиндализированном образце.
2. Способ по п. 1, где к 0,5 мл образца, содержащего тиндализированные бактериальные клетки Lactobacillus rhamnosus GG (АТСС 53103) в концентрации от 105 до 107 клеток/мл, добавляют 2,5 микролитра указанного первого реагента и 1,5 микролитра указанного второго реагента с образованием раствора.
3. Способ по п. 1 или 2, где суспензия флуоресцентных микросфер в азиде натрия содержит микросферы из полистирола, предпочтительно суспензия шариков представляет собой суспензию флуоресцентных микросфер из полистирола в 0,1%-ном растворе азида натрия.
4. Способ по п. 3, где известное количество шариков, составляющее от 10 до 100 мкл, предпочтительно от 40 до 60 мкл, добавляют для обеспечения возможности определения количества клеток.
5. Способ получения тиндализированных бактериальных клеток Lactobacillus rhamnosus GG (АТСС 53103), имеющих интактную клеточную стенку, включающий:
- получение бактериальной культуры клеток Lactobacillus rhamnosus GG (АТСС 53103), имеющей концентрацию от 1×106 до 1×1010 КОЕ/г;
- осуществление над указанной культурой процесса тиндализации с получением культуры тиндализированных бактериальных клеток;
- применение способа подсчета по любому из пп. 1-4.
6. Культура тиндализированных, интактных и иммунологически активных бактериальных клеток Lactobacillus rhamnosus GG (АТСС 53103), полученная способом по п. 5.
7. Применение проточной цитофлюорометрии для подсчета количества тиндализированных мертвых бактериальных клеток Lactobacillus rhamnosus GG (АТСС 53103), имеющих интактную клеточную мембрану, в образце тиндализированных бактериальных клеток Lactobacillus rhamnosus GG (АТСС 53103).
8. Применение по п. 7, где указанные тиндализированные мертвые бактериальные клетки Lactobacillus rhamnosus GG (АТСС 53103), имеющие интактную клеточную мембрану, подсчитаны способом по любому из пп. 1-4.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITMI20141951 | 2014-11-12 | ||
| IT102014902308600 | 2014-11-12 | ||
| PCT/IB2015/058747 WO2016075649A1 (en) | 2014-11-12 | 2015-11-12 | Preparation of tyndallized, intact and immunologically active cells of lactobacillus rhamnosus gg and method for qualitative and quantitative determination thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2017117030A true RU2017117030A (ru) | 2018-12-13 |
| RU2017117030A3 RU2017117030A3 (ru) | 2019-05-29 |
Family
ID=52444429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017117030A RU2017117030A (ru) | 2014-11-12 | 2015-11-12 | Получение тиндализированных, интактных и иммунологически активных клеток lactobacillus rhamnosus GG и способ их качественного и количественного определения |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20170322140A1 (ru) |
| EP (1) | EP3218692A1 (ru) |
| JP (1) | JP2018502557A (ru) |
| KR (1) | KR20170081187A (ru) |
| CN (1) | CN107003226A (ru) |
| BR (1) | BR112017009450A2 (ru) |
| CA (1) | CA2965446A1 (ru) |
| RU (1) | RU2017117030A (ru) |
| WO (1) | WO2016075649A1 (ru) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201719480D0 (en) * | 2017-11-23 | 2018-01-10 | Optibiotix Ltd | Compositions |
| IT202100016055A1 (it) * | 2021-06-18 | 2022-12-18 | Univ Degli Studi Di Camerino | Composizione comprendente estratto secco di contenuto intestinale di polli adulti liofilizzato, relativo uso come integratore alimentare e relativo uso per stimolare il sistema immunitario |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040023319A1 (en) * | 2002-03-26 | 2004-02-05 | Godfrey William L. | Rapid enumeration of viable spores by flow cytometry |
| IT1398730B1 (it) * | 2009-09-08 | 2013-03-18 | Giuliani Spa | Ceppo batterico probiotico e suoi usi per via orale e topica |
| CN103789397A (zh) * | 2012-10-30 | 2014-05-14 | 北京普析通用仪器有限责任公司 | 检测细菌总数的试剂盒和检测方法 |
-
2015
- 2015-11-12 CN CN201580060229.7A patent/CN107003226A/zh active Pending
- 2015-11-12 WO PCT/IB2015/058747 patent/WO2016075649A1/en not_active Ceased
- 2015-11-12 US US15/524,982 patent/US20170322140A1/en not_active Abandoned
- 2015-11-12 EP EP15820605.2A patent/EP3218692A1/en not_active Ceased
- 2015-11-12 KR KR1020177013321A patent/KR20170081187A/ko not_active Ceased
- 2015-11-12 BR BR112017009450A patent/BR112017009450A2/pt not_active Application Discontinuation
- 2015-11-12 RU RU2017117030A patent/RU2017117030A/ru not_active Application Discontinuation
- 2015-11-12 JP JP2017525576A patent/JP2018502557A/ja active Pending
- 2015-11-12 CA CA2965446A patent/CA2965446A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20170322140A1 (en) | 2017-11-09 |
| JP2018502557A (ja) | 2018-02-01 |
| CN107003226A (zh) | 2017-08-01 |
| BR112017009450A2 (pt) | 2017-12-19 |
| WO2016075649A1 (en) | 2016-05-19 |
| KR20170081187A (ko) | 2017-07-11 |
| EP3218692A1 (en) | 2017-09-20 |
| CA2965446A1 (en) | 2016-05-19 |
| RU2017117030A3 (ru) | 2019-05-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Oeschger et al. | Point of care technologies for sepsis diagnosis and treatment | |
| ES2477588T3 (es) | Ensayo de activación de monocitos perfeccionado capaz de detectar contaminantes pirogénicos no endotoxínicos en productos médicos | |
| CN103869060B (zh) | 基于磁珠和微流控芯片的循环肿瘤干细胞检测试剂盒 | |
| JP2016506519A5 (ru) | ||
| Langer et al. | Rapid quantification of bioaerosols containing L. pneumophila by Coriolis® μ air sampler and chemiluminescence antibody microarrays | |
| Cox et al. | Rapid detection of Bacillus anthracis by γ phage amplification and lateral flow immunochromatography | |
| CN202393769U (zh) | 一种梳式斑点酶联免疫多项病毒检测试剂盒 | |
| RU2017117030A (ru) | Получение тиндализированных, интактных и иммунологически активных клеток lactobacillus rhamnosus GG и способ их качественного и количественного определения | |
| CN103760345B (zh) | 一种利用外周血检测结核分枝杆菌感染的试剂盒及其应用 | |
| Rocha-Silva et al. | Histoplasmosis outbreak in Tamboril cave—Minas Gerais state, Brazil | |
| Liedig et al. | Blood supplementation enhances Bartonella henselae growth and molecular detection of bacterial DNA in liquid culture | |
| CN106386785B (zh) | 一种细胞保存液及其在流式样本保存中的应用 | |
| RU2015146401A (ru) | Способ исследования кала на дисбактериоз кишечника | |
| Schwaminger et al. | Detection of targeted bacteria species on filtration membranes | |
| CN110447634A (zh) | 一种猴红细胞保存液及应用 | |
| CN104181093A (zh) | 番茄溃疡病菌有活力菌体的流式细胞术计数方法 | |
| Chalin et al. | A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths | |
| RU2505819C1 (ru) | СПОСОБ КОНСТРУИРОВАНИЯ ПОЛИМЕРНОГО ИММУНОГЛОБУЛИНОВОГО ДИАГНОСТИКУМА ДЛЯ ВЫЯВЛЕНИЯ Legionella pneumophila 1,3 И 6 СЕРОГРУПП (ВАРИАНТЫ) | |
| Patra et al. | Are 3D spheroids always more resistant to chemotherapy than 2D cultures? A chip-based survey using ovarian cancer cell lines | |
| RU2634868C2 (ru) | Состав агарового покрытия для определения концентрации и состава популяции вируса мачупо - возбудителя боливийской геморрагической лихорадки с использованием метода негативных колоний | |
| Bharti et al. | An update on diagnosis of tuberculosis: A review | |
| HRP20192036T1 (hr) | Tekući hranjivi medij za uzgoj klostridija štetnih za proizvodnju sira i postupak njihove detekcije | |
| CN204855529U (zh) | 一种ii型志贺毒素时间分辨荧光免疫检测试剂盒 | |
| Gonçalves et al. | Protozoan infection in archaeological material | |
| Abioje et al. | Serological Survey of Equine and Avian Influenza Viruses in Horses in Zaria, Kaduna State |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20210621 |