RU2015106500A - NEW INSECTICIDAL PROTEINS AND WAYS OF THEIR APPLICATION - Google Patents
NEW INSECTICIDAL PROTEINS AND WAYS OF THEIR APPLICATION Download PDFInfo
- Publication number
- RU2015106500A RU2015106500A RU2015106500A RU2015106500A RU2015106500A RU 2015106500 A RU2015106500 A RU 2015106500A RU 2015106500 A RU2015106500 A RU 2015106500A RU 2015106500 A RU2015106500 A RU 2015106500A RU 2015106500 A RU2015106500 A RU 2015106500A
- Authority
- RU
- Russia
- Prior art keywords
- xaa
- leu
- glu
- val
- asp
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims 4
- 108090000623 proteins and genes Proteins 0.000 title claims 4
- 230000000749 insecticidal effect Effects 0.000 title claims 3
- 229920001184 polypeptide Polymers 0.000 claims abstract 39
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 39
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 39
- 102000039446 nucleic acids Human genes 0.000 claims abstract 26
- 108020004707 nucleic acids Proteins 0.000 claims abstract 26
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 26
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 10
- 230000009261 transgenic effect Effects 0.000 claims 21
- 241000238631 Hexapoda Species 0.000 claims 12
- 241000607479 Yersinia pestis Species 0.000 claims 12
- 238000000034 method Methods 0.000 claims 8
- 230000001276 controlling effect Effects 0.000 claims 5
- 150000001413 amino acids Chemical class 0.000 claims 4
- 230000001105 regulatory effect Effects 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 230000000361 pesticidal effect Effects 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/225—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Alcaligenes (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
1. Выделенная молекула нуклеиновой кислоты, кодирующая полипептид AfIP-1A.2. Выделенная молекула нуклеиновой кислоты по п. 1, где полипептид AfIP-1A по меньшей мере на 80% идентичен аминокислотной последовательности с SEQ ID NO: 2.3. Выделенная молекула нуклеиновой кислоты по п. 2, где полипептид AfIP-1A содержит аминокислотную последовательность с формулойгде Xaa в положении 6 представляет собой Ile, Thr, Leu, Met, Val или Ser;Xaa в положении 7 представляет собой Ala, Met, Val, Leu, Ile или Gly;Xaa в положении 8 представляет собой Thr, Asp, Ser или Glu;Xaa в положении 9 представляет собой Glu, Leu, Gly, Asp, Ala, Ile, Val или Met;Xaa в положении 10 представляет собой Glu, Asn, Asp или Gln;Xaa в положении 11 представляет собой Ser, Val, Thr, Ile, Leu или Met;Xaa в положении 12 представляет собой Lys, Glu, Arg или Asp;Xaa в положении 13 представляет собой Ile, Val, Leu или Met;Xaa в положении 14 представляет собой Arg, Gln, Lys или Asn;Xaa в положении 16 представляет собой Tyr, Gln, Trp, Phe или Asn;Xaa в положении 17 представляет собой Ala, Ser, Gly или Thr;Xaa в положении 19 представляет собой Trp, Glu, Phe, Ile, His, Asn или Tyr;Xaa в положении 20 представляет собой Ile, Val, Ala, Cys, Glu, Phe, Gly, Met, Asn, Gln, Arg, Ser или Thr;Xaa в положении 23 представляет собой Thr, Glu, Ala, Ser, Asp или Gly;Xaa в положении 24 представляет собой Ile, Leu, Val или Met;Xaa в положении 26 представляет собой Val, Ser, Ile, Leu, Met или Thr;Xaa в положении 27 представляет собой Val, Glu, Ile, Leu, Met или Asp;Xaa в положении 29 представляет собой Ser, Met, Thr, Ile, Leu или Val;Xaa в положении 30 представляет собой Asn, Asp, Ser, Glu, Gln или Thr;Xaa в положении 31 представляет собой Phe, Ile, Leu, Val или Met;Xaa в положении 32 представляет собой Lys, Glu, Arg илиAsp;Xaa в положении 33 представляет собой Val, Ile, Leu или Met;Xaa в положении 34 представляет собой Glu, Lys, Asp или Arg;Xaa в положении 35 представляет собой Gly или Asn;Xaa в положении 36 представляет собой Ala, Gly, Asp, Glu, Phe, Gly, Ile, Leu, Pro, Gln, Arg, Ser, Thr, Val, Trp или Tyr;Xaa в положении 37 представляет собой Tyr, Ala, Cys, Asp, Glu, Gly, His, Ile, Lys, Leu, Met, Pro, Arg, Ser, Thr, Val или Trp;Xaa в положении 38 представляет собой Leu, Ala, Cys, Asp, Glu, Phe, Gly, His, Ile, Lys, Met, Gln, Arg, Ser, Thr, Val, Trp или Tyr;Xaa в положении 39 представляет собой Arg, Lys, Cys, Asp, Glu, Phe, Gly, Ile, Lys, Leu, Met, Asn, Pro, Ser, Thr, Val, Trp или Tyr;Xaa в положении 40 представляет собой Trp, Ala, Cys, Asp, Glu, Phe, Gly, His, Ile, Lys, Leu, Met, Asn, Pro, Gln, Arg, Ser, Thr, Val или Tyr;Xaa в положении 41 представляет собой Gly, Cys или Gln;Xaa в положении 42 представляет собой Lys, Cys, Glu, His, Leu, Met, Asn, Gln, Arg или Thr;Xaa в1. An isolated nucleic acid molecule encoding an AfIP-1A.2 polypeptide. The isolated nucleic acid molecule of claim 1, wherein the AfIP-1A polypeptide is at least 80% identical to the amino acid sequence with SEQ ID NO: 2.3. The isolated nucleic acid molecule of claim 2, wherein the AfIP-1A polypeptide contains an amino acid sequence of the formula where Xaa at position 6 is Ile, Thr, Leu, Met, Val or Ser; Xaa at position 7 is Ala, Met, Val, Leu , Ile or Gly; Xaa at position 8 is Thr, Asp, Ser or Glu; Xaa at position 9 is Glu, Leu, Gly, Asp, Ala, Ile, Val or Met; Xaa at position 10 is Glu, Asn , Asp or Gln; Xaa at position 11 is Ser, Val, Thr, Ile, Leu or Met; Xaa at position 12 is Lys, Glu, Arg or Asp; Xaa at position 13 is Ile, Val, Leu or Met ; X aa at position 14 is Arg, Gln, Lys or Asn; Xaa at position 16 is Tyr, Gln, Trp, Phe or Asn; Xaa at position 17 is Ala, Ser, Gly or Thr; Xaa at position 19 is Trp, Glu, Phe, Ile, His, Asn or Tyr; Xaa at position 20 is Ile, Val, Ala, Cys, Glu, Phe, Gly, Met, Asn, Gln, Arg, Ser or Thr; Xaa at position 23 represents Thr, Glu, Ala, Ser, Asp or Gly; Xaa at position 24 represents Ile, Leu, Val or Met; Xaa at position 26 represents Val, Ser, Ile, Leu, Met or Thr; Xaa at position 27 represents Val, Glu, Ile, Leu, Met or Asp; Xaa at position 29 represents Ser, Met, Thr, Ile, Leu or Val; Xaa at position 30 is Asn, Asp, Ser, Glu, Gln or Thr; Xaa at position 31 is Phe, Ile, Leu, Val or Met; Xaa at position 32 is Lys, Glu, Arg or Asp; Xaa at position 33 is Val, Ile, Leu or Met; Xaa at position 34 is Glu, Lys, Asp or Arg; Xaa at position 35 is Gly or Asn; Xaa at position 36 is Ala, Gly, Asp, Glu , Phe, Gly, Ile, Leu, Pro, Gln, Arg, Ser, Thr, Val, Trp or Tyr; Xaa at position 37 is Tyr, Ala, Cys, Asp, Glu, Gly, His, Ile, Lys, Leu , Met, Pro, Arg, Ser, Thr, Val or Trp; Xaa at position 38 is Leu, Ala, Cys, Asp, Glu, Phe, Gly, His, Ile, Lys, Met, Gln, Arg, Ser, Thr , Val, Tr p or Tyr; Xaa at position 39 is Arg, Lys, Cys, Asp, Glu, Phe, Gly, Ile, Lys, Leu, Met, Asn, Pro, Ser, Thr, Val, Trp or Tyr; Xaa at position 40 represents Trp, Ala, Cys, Asp, Glu, Phe, Gly, His, Ile, Lys, Leu, Met, Asn, Pro, Gln, Arg, Ser, Thr, Val or Tyr; Xaa at position 41 represents Gly, Cys or Gln; Xaa at position 42 is Lys, Cys, Glu, His, Leu, Met, Asn, Gln, Arg or Thr; Xaa at
Claims (39)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261675950P | 2012-07-26 | 2012-07-26 | |
| US61/675,950 | 2012-07-26 | ||
| US201261739468P | 2012-12-19 | 2012-12-19 | |
| US61/739,468 | 2012-12-19 | ||
| US201361779457P | 2013-03-13 | 2013-03-13 | |
| US61/779,457 | 2013-03-13 | ||
| PCT/US2013/052254 WO2014018853A1 (en) | 2012-07-26 | 2013-07-26 | Novel insecticidal proteins and methods for their use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2015106500A true RU2015106500A (en) | 2016-09-20 |
| RU2666914C2 RU2666914C2 (en) | 2018-09-13 |
Family
ID=48949237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015106500A RU2666914C2 (en) | 2012-07-26 | 2013-07-26 | Novel insecticidal proteins and methods of their use |
Country Status (7)
| Country | Link |
|---|---|
| CN (1) | CN105980566A (en) |
| AR (1) | AR091910A1 (en) |
| BR (1) | BR112015001741A2 (en) |
| CA (1) | CA2880226C (en) |
| MX (2) | MX383071B (en) |
| RU (1) | RU2666914C2 (en) |
| WO (1) | WO2014018853A1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA126192C2 (en) * | 2014-10-16 | 2022-08-31 | Піонір Хай-Бред Інтернешнл, Інк. | INSECTICIDAL PROTEIN AND METHOD OF ITS APPLICATION |
| AU2019321983B2 (en) * | 2018-08-13 | 2025-11-20 | Aarhus Universitet | Genetically altered LysM receptors with altered agonist specificity and affinity |
| CN110055230B (en) * | 2019-04-17 | 2021-06-15 | 凯莱英生命科学技术(天津)有限公司 | Monooxygenase mutants and their applications |
| CN113234724B (en) * | 2021-05-18 | 2022-04-05 | 华中农业大学 | A dsRNA for controlling termites and its application |
| CN114990110B (en) * | 2022-07-19 | 2023-07-14 | 江西农业大学 | A non-destructive sampling method for butterfly monitoring in the wild |
| CN115684506A (en) * | 2022-10-20 | 2023-02-03 | 中国林业科学研究院林产化学工业研究所 | Rapid quantitative evaluation method based on binary antioxidant combination effect |
| WO2025052302A1 (en) | 2023-09-05 | 2025-03-13 | Mazen Animal Health, Inc. | Methods and compositions for the production of mannanase in plants |
| WO2025074304A1 (en) | 2023-10-03 | 2025-04-10 | Mazen Animal Health, Inc. | Compositions and methods for in planta production of a porcine circovirus vaccine |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| CN1684974A (en) * | 2002-06-28 | 2005-10-19 | 美国陶氏益农公司 | Insecticidally active proteins and polynucleotides obtainable from Paenibacillus species |
| US7491698B2 (en) * | 2003-01-21 | 2009-02-17 | Dow Agrosciences Llc | Mixing and matching TC proteins for pest control |
| US7244607B2 (en) * | 2003-10-01 | 2007-07-17 | The United States Of America, As Represented By The Secretary Of Agriculture | Chromobacterium subtsugae sp. nov. for control of insect pests |
| WO2008142036A2 (en) * | 2007-05-22 | 2008-11-27 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko |
| US8853490B2 (en) * | 2010-10-26 | 2014-10-07 | Pioneer Hi Bred International Inc | Antifungal proteins and methods of use |
-
2013
- 2013-07-25 AR ARP130102664A patent/AR091910A1/en unknown
- 2013-07-26 MX MX2018003716A patent/MX383071B/en unknown
- 2013-07-26 MX MX2015001046A patent/MX357713B/en active IP Right Grant
- 2013-07-26 BR BR112015001741A patent/BR112015001741A2/en not_active Application Discontinuation
- 2013-07-26 CA CA2880226A patent/CA2880226C/en not_active Expired - Fee Related
- 2013-07-26 CN CN201380050024.1A patent/CN105980566A/en active Pending
- 2013-07-26 WO PCT/US2013/052254 patent/WO2014018853A1/en not_active Ceased
- 2013-07-26 RU RU2015106500A patent/RU2666914C2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2880226C (en) | 2019-07-16 |
| CA2880226A1 (en) | 2014-01-30 |
| CN105980566A (en) | 2016-09-28 |
| MX357713B (en) | 2018-07-20 |
| WO2014018853A1 (en) | 2014-01-30 |
| MX2015001046A (en) | 2015-10-20 |
| BR112015001741A2 (en) | 2017-08-08 |
| AR091910A1 (en) | 2015-03-11 |
| RU2666914C2 (en) | 2018-09-13 |
| MX383071B (en) | 2025-03-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20200727 |