RU2012125394A - METHOD FOR THIN-LAYER CULTIVATION OF HELICOBACTER PYLORI AND TWO-PHASE NUTRIENT ENVIRONMENT FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR THIN-LAYER CULTIVATION OF HELICOBACTER PYLORI AND TWO-PHASE NUTRIENT ENVIRONMENT FOR ITS IMPLEMENTATION Download PDFInfo
- Publication number
- RU2012125394A RU2012125394A RU2012125394/10A RU2012125394A RU2012125394A RU 2012125394 A RU2012125394 A RU 2012125394A RU 2012125394/10 A RU2012125394/10 A RU 2012125394/10A RU 2012125394 A RU2012125394 A RU 2012125394A RU 2012125394 A RU2012125394 A RU 2012125394A
- Authority
- RU
- Russia
- Prior art keywords
- agar
- thin
- helicobacter pylori
- layer cultivation
- broth
- Prior art date
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- 241000590002 Helicobacter pylori Species 0.000 title claims abstract 9
- 229940037467 helicobacter pylori Drugs 0.000 title claims abstract 9
- 235000015097 nutrients Nutrition 0.000 title 1
- 239000007330 chocolate agar Substances 0.000 claims abstract 6
- 239000001963 growth medium Substances 0.000 claims abstract 6
- 239000004615 ingredient Substances 0.000 claims abstract 6
- 229920001817 Agar Polymers 0.000 claims abstract 5
- 239000012071 phase Substances 0.000 claims abstract 5
- 241000283690 Bos taurus Species 0.000 claims abstract 4
- 239000008272 agar Substances 0.000 claims abstract 4
- 210000004369 blood Anatomy 0.000 claims abstract 4
- 239000008280 blood Substances 0.000 claims abstract 4
- 239000007382 columbia agar Substances 0.000 claims abstract 4
- 210000002966 serum Anatomy 0.000 claims abstract 4
- 239000007790 solid phase Substances 0.000 claims abstract 4
- 239000007788 liquid Substances 0.000 claims abstract 2
- 239000007791 liquid phase Substances 0.000 claims abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 4
- 235000010419 agar Nutrition 0.000 claims 3
- 239000011780 sodium chloride Substances 0.000 claims 2
- 229920002261 Corn starch Polymers 0.000 claims 1
- 241000206672 Gelidium Species 0.000 claims 1
- XKVYZLLWKHGKMT-BEJOYRPXSA-N Gemin D Natural products O([C@@H]([C@@H](O)C=O)[C@@H]1[C@@H](O)COC(=O)c2c(c(O)c(O)c(O)c2)-c2c(O)c(O)c(O)cc2C(=O)O1)C(=O)c1cc(O)c(O)c(O)c1 XKVYZLLWKHGKMT-BEJOYRPXSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- 239000004158 L-cystine Substances 0.000 claims 1
- 235000019393 L-cystine Nutrition 0.000 claims 1
- 108090000526 Papain Proteins 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- 235000015278 beef Nutrition 0.000 claims 1
- 229940041514 candida albicans extract Drugs 0.000 claims 1
- 239000005018 casein Substances 0.000 claims 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims 1
- 235000021240 caseins Nutrition 0.000 claims 1
- 239000008120 corn starch Substances 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims 1
- 235000019797 dipotassium phosphate Nutrition 0.000 claims 1
- 235000013312 flour Nutrition 0.000 claims 1
- 229930192479 gemin Natural products 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 claims 1
- 229940055729 papain Drugs 0.000 claims 1
- 235000019834 papain Nutrition 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
- 239000012138 yeast extract Substances 0.000 claims 1
- 230000002051 biphasic effect Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Способ тонкослойного культивирования Helicobacter pylori, предусматривающий использование шоколадного агара, изготовленного на основе Колумбия агара с добавлением 5% крови барана, отличающийся тем, что к шоколадному агару добавляют 3,5 мл бульона Шедлера, обогащенного 10% сывороткой крупного рогатого скота.2. Двухфазная питательная среда для тонкослойного культивирования Helicobacter pylori, содержащая твердую фазу, представленную шоколадным агаром, изготовленным на основе Колумбия агара с 5% крови барана, и жидкую фазу, представляющую собой бульон Шедлера, обогащенный 10% сывороткой крупного рогатого скота, отличающаяся тем, что соотношение твердой фазы к жидкой составляет 4:1 (14 мл агара и 3,5 мл бульона).3. Двухфазная питательная среда для тонкослойного культивирования Helicobacter pylori по пункту 2, отличающаяся тем, что Колумбия агар содержит следующие ингредиенты в г/литр:4. Двухфазная питательная среда для тонкослойного культивирования Helicobacter pylori по пункту 2, отличающаяся тем, что бульон Шедлера содержит следующие ингредиенты в г/литр:1. A method of thin-layer cultivation of Helicobacter pylori, involving the use of chocolate agar made on the basis of Columbia agar with the addition of 5% ram blood, characterized in that 3.5 ml of Shedler broth enriched with 10% cattle serum is added to chocolate agar. A two-phase culture medium for thin-layer cultivation of Helicobacter pylori, containing a solid phase represented by chocolate agar made on the basis of Columbia agar with 5% ram blood, and a liquid phase, which is a Shedler broth enriched with 10% cattle serum, characterized in that the ratio the solid phase to liquid is 4: 1 (14 ml of agar and 3.5 ml of broth) .3. The biphasic culture medium for thin-layer cultivation of Helicobacter pylori according to paragraph 2, characterized in that Colombia agar contains the following ingredients in g / liter: 4. The two-phase culture medium for thin-layer cultivation of Helicobacter pylori according to paragraph 2, characterized in that the Shedler broth contains the following ingredients in g / liter:
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2012125394/10A RU2518304C2 (en) | 2012-06-20 | 2012-06-20 | TWO-PHASE NUTRITIONAL MEDIUM FOR THIN LAYER CULTIVATION OF Helicobacter pylori AND METHOD OF ITS REALISATION |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2012125394/10A RU2518304C2 (en) | 2012-06-20 | 2012-06-20 | TWO-PHASE NUTRITIONAL MEDIUM FOR THIN LAYER CULTIVATION OF Helicobacter pylori AND METHOD OF ITS REALISATION |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2012125394A true RU2012125394A (en) | 2013-12-27 |
| RU2518304C2 RU2518304C2 (en) | 2014-06-10 |
Family
ID=49785766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012125394/10A RU2518304C2 (en) | 2012-06-20 | 2012-06-20 | TWO-PHASE NUTRITIONAL MEDIUM FOR THIN LAYER CULTIVATION OF Helicobacter pylori AND METHOD OF ITS REALISATION |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2518304C2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108424867A (en) * | 2018-04-18 | 2018-08-21 | 深圳市伯劳特生物制品有限公司 | composition and culture medium containing the composition |
| CN108486211A (en) * | 2018-04-18 | 2018-09-04 | 深圳市伯劳特生物制品有限公司 | Helicobacter pylori drug susceptibility detection medium and kit |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2678123C1 (en) * | 2017-10-13 | 2019-01-23 | Федеральное государственное бюджетное учреждение науки Институт клеточного и внутриклеточного симбиоза Уральского отделения Российской академии наук | Nutrient environment for restoring number of anaerobic bacteria after low-temperature storage |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020026035A1 (en) * | 1997-04-01 | 2002-02-28 | Harold Kleanthous | Helicobacter ghpo 1360 and ghpo 750 polypeptides and corresponding polynucleotide molecules |
| RU2145975C1 (en) * | 1999-07-19 | 2000-02-27 | Тверская медакадемия | Method of culturing helicobacter pilori |
-
2012
- 2012-06-20 RU RU2012125394/10A patent/RU2518304C2/en active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108424867A (en) * | 2018-04-18 | 2018-08-21 | 深圳市伯劳特生物制品有限公司 | composition and culture medium containing the composition |
| CN108486211A (en) * | 2018-04-18 | 2018-09-04 | 深圳市伯劳特生物制品有限公司 | Helicobacter pylori drug susceptibility detection medium and kit |
| CN108424867B (en) * | 2018-04-18 | 2021-11-26 | 深圳市伯劳特生物制品有限公司 | Composition and culture medium containing same |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2518304C2 (en) | 2014-06-10 |
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