RU2011149592A - METHOD FOR PRODUCING POLYMIXIN B PHARMACEUTICAL SUBSTANCE - Google Patents
METHOD FOR PRODUCING POLYMIXIN B PHARMACEUTICAL SUBSTANCE Download PDFInfo
- Publication number
- RU2011149592A RU2011149592A RU2011149592/02A RU2011149592A RU2011149592A RU 2011149592 A RU2011149592 A RU 2011149592A RU 2011149592/02 A RU2011149592/02 A RU 2011149592/02A RU 2011149592 A RU2011149592 A RU 2011149592A RU 2011149592 A RU2011149592 A RU 2011149592A
- Authority
- RU
- Russia
- Prior art keywords
- polymyxin
- solution
- producing
- exchange resin
- sulfate
- Prior art date
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- 239000003814 drug Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 108010093965 Polymyxin B Proteins 0.000 claims abstract 18
- 229920000024 polymyxin B Polymers 0.000 claims abstract 12
- 229960005266 polymyxin b Drugs 0.000 claims abstract 12
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract 6
- SBKRTALNRRAOJP-BWSIXKJUSA-N N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methylheptanamide (6S)-N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methyloctanamide sulfuric acid Polymers OS(O)(=O)=O.CC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O.CC[C@H](C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O SBKRTALNRRAOJP-BWSIXKJUSA-N 0.000 claims abstract 6
- 238000000034 method Methods 0.000 claims abstract 6
- 229960003548 polymyxin b sulfate Drugs 0.000 claims abstract 6
- 238000001035 drying Methods 0.000 claims abstract 4
- 239000003456 ion exchange resin Substances 0.000 claims abstract 4
- 229920003303 ion-exchange polymer Polymers 0.000 claims abstract 4
- 239000002028 Biomass Substances 0.000 claims abstract 2
- 241000194105 Paenibacillus polymyxa Species 0.000 claims abstract 2
- 239000004793 Polystyrene Substances 0.000 claims abstract 2
- 230000002378 acidificating effect Effects 0.000 claims abstract 2
- 239000003463 adsorbent Substances 0.000 claims abstract 2
- 239000003729 cation exchange resin Substances 0.000 claims abstract 2
- 150000001768 cations Chemical class 0.000 claims abstract 2
- 239000012531 culture fluid Substances 0.000 claims abstract 2
- 238000010828 elution Methods 0.000 claims abstract 2
- 229920002223 polystyrene Polymers 0.000 claims abstract 2
- 238000001556 precipitation Methods 0.000 claims abstract 2
- 238000000746 purification Methods 0.000 claims abstract 2
- 238000000926 separation method Methods 0.000 claims abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
1. Способ получения полимиксина В, продуцируемого спорообразующими бактериями Bacillus polymyxa путем очистки нативного раствора, получаемого после отделения биомассы от культуральной жидкости, с использованием неионогенных адсорбентов полистирольного типа с определенными поверхностными параметрами, с элюированием полимиксина B, осаждением полимиксина B основания с последующим переводом его в раствор полимиксина B сульфат с его последующей сушкой, отличающийся тем, что нативный раствор полимиксина B очищают с использованием макропористого сильнокислотного катионита, с последующей обработкой раствора полимиксина B сульфата перед сушкой ионообменной смолой.2. Способ получения полимиксина B по п.1, отличающийся тем, что в качестве макропористого сильнокислотного катионита используется Dowex-MSC-1.3. Способ получения полимиксина B по п.1, отличающийся тем, что в качестве ионообменной смолы для обработки финишного раствора полимиксина B сульфата используется КУ-2-20.1. A method of producing polymyxin B produced by the spore-forming bacteria Bacillus polymyxa by purification of the native solution obtained after separation of biomass from the culture fluid using non-ionic polystyrene adsorbents with certain surface parameters, with elution of polymyxin B, precipitation of polymyxin B base, followed by its transfer to polymyxin B sulfate solution with its subsequent drying, characterized in that the native polymyxin B solution is purified using macroporous c hydrochloric acid cation exchange resin, followed by treatment with a solution of polymyxin B sulfate before drying with an ion exchange resin. 2. The method of producing polymyxin B according to claim 1, characterized in that Dowex-MSC-1.3 is used as a macroporous strongly acidic cation exchanger. The method of producing polymyxin B according to claim 1, characterized in that KU-2-20 is used as an ion exchange resin for processing the final solution of polymyxin B sulfate.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2011149592A true RU2011149592A (en) | 2013-06-20 |
| RU2492180C2 RU2492180C2 (en) | 2013-09-10 |
Family
ID=48784904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2492180C2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018103758A1 (en) * | 2016-12-09 | 2018-06-14 | 上海医药工业研究院 | High-titer polymyxin e2 sodium methanesulfonate |
| CN116535471A (en) * | 2023-05-05 | 2023-08-04 | 丽珠集团福州福兴医药有限公司 | RRT0.67 impurity in polymyxin B sulfate and separation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108977482B (en) * | 2018-08-21 | 2020-11-24 | 浙江孚诺医药股份有限公司 | Preparation method of polymyxin B sulfate |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB782926A (en) * | 1955-01-06 | 1957-09-18 | Distillers Co Yeast Ltd | Purification of polymyxin |
| SU111195A1 (en) * | 1957-06-26 | 1957-11-30 | С.М. Мамиофе | Method for producing polymyxin antibiotic |
| GB991602A (en) * | 1961-03-10 | 1965-05-12 | Wellcome Found | Antibiotics and their purification |
| SK287315B6 (en) * | 2006-06-02 | 2010-06-07 | Biotika, A. S. | A method for polymyxin B isolation from fermented soil |
-
2011
- 2011-12-07 RU RU2011149592/10A patent/RU2492180C2/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018103758A1 (en) * | 2016-12-09 | 2018-06-14 | 上海医药工业研究院 | High-titer polymyxin e2 sodium methanesulfonate |
| CN116535471A (en) * | 2023-05-05 | 2023-08-04 | 丽珠集团福州福兴医药有限公司 | RRT0.67 impurity in polymyxin B sulfate and separation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2492180C2 (en) | 2013-09-10 |
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