RU2010112423A - Способы переноса молекулярных веществ в клетки растений - Google Patents
Способы переноса молекулярных веществ в клетки растений Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract 30
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- 239000011248 coating agent Substances 0.000 claims abstract 11
- 238000000576 coating method Methods 0.000 claims abstract 11
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- 210000003763 chloroplast Anatomy 0.000 claims abstract 5
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims abstract 4
- 238000010521 absorption reaction Methods 0.000 claims abstract 4
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 claims abstract 3
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract 3
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 claims abstract 3
- 241001133760 Acoelorraphe Species 0.000 claims abstract 2
- 241001149092 Arabidopsis sp. Species 0.000 claims abstract 2
- 235000017060 Arachis glabrata Nutrition 0.000 claims abstract 2
- 244000105624 Arachis hypogaea Species 0.000 claims abstract 2
- 235000010777 Arachis hypogaea Nutrition 0.000 claims abstract 2
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- 229920000742 Cotton Polymers 0.000 claims abstract 2
- 244000000626 Daucus carota Species 0.000 claims abstract 2
- 235000002767 Daucus carota Nutrition 0.000 claims abstract 2
- 241000219146 Gossypium Species 0.000 claims abstract 2
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract 2
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract 2
- 241000511986 Oryza sp. Species 0.000 claims abstract 2
- 235000003846 Ricinus Nutrition 0.000 claims abstract 2
- 241000322381 Ricinus <louse> Species 0.000 claims abstract 2
- 240000000111 Saccharum officinarum Species 0.000 claims abstract 2
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract 2
- 240000008042 Zea mays Species 0.000 claims abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 2
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- 108090000623 proteins and genes Proteins 0.000 claims 8
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 3
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 230000014509 gene expression Effects 0.000 claims 2
- 239000013612 plasmid Substances 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 240000002791 Brassica napus Species 0.000 claims 1
- 244000188595 Brassica sinapistrum Species 0.000 claims 1
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- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims 1
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- 240000000385 Brassica napus var. napus Species 0.000 abstract 2
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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Abstract
1. Способ введения представляющей интерес молекулы в клетку растения, имеющую клеточную стенку, предусматривающий ! обеспечение клетки растения, имеющей клеточную стенку; ! покрытие наночастицы представляющей интерес молекулой; ! приведение клетки, имеющей клеточную стенку, и имеющей покрытие наночастицы в контакт друг с другом; и ! позволение поглощения наночастицы и представляющей интерес молекулы в клетку, содержащую клеточную стенку. ! 2. Способ по п.1, где покрытие наночастицы представляющей интерес молекулой предусматривает иммобилизацию представляющей интерес молекулы посредством нековалентной абсорбции на поверхности наночастицы. ! 3. Способ по п.1, дополнительно предусматривающий абсорбцию представляющей интерес молекулы в наночастицу. !4. Способ по п.1, дополнительно предусматривающий позволение поглощения наночастицы в компартмент клетки растения, содержащей клеточную стенку. ! 5. Способ по п.4, дополнительно предусматривающий покрытие наночастицы нацеливающим на субклеточный компартмент белком. ! 6. Способ по п.4, где компартмент выбран из группы, состоящей из цитозоля, ядра, тонопластов, пластиды, этиопласта, хромопласта, лейкопласта, элайопласта, протеинопласта, амилопласта, хлоропласта и просвета двойной мембраны. ! 7. Способ по п.1, где клетка растения, содержащая клеточную стенку, выбрана из группы, состоящей из клеток табака, моркови, кукурузы, канолы, рапса, хлопчатника, пальмы, арахиса, сои, Oryza sp., Arabidopsis sp., Ricinus sp. и сахарного тростника. ! 8. Способ по п.1, где клетка растения происходит из ткани, выбранной из группы, состоящей из зародыша, меристемы, каллуса, пыльцы, листьев, пыльников, корней, кон�
Claims (21)
1. Способ введения представляющей интерес молекулы в клетку растения, имеющую клеточную стенку, предусматривающий
обеспечение клетки растения, имеющей клеточную стенку;
покрытие наночастицы представляющей интерес молекулой;
приведение клетки, имеющей клеточную стенку, и имеющей покрытие наночастицы в контакт друг с другом; и
позволение поглощения наночастицы и представляющей интерес молекулы в клетку, содержащую клеточную стенку.
2. Способ по п.1, где покрытие наночастицы представляющей интерес молекулой предусматривает иммобилизацию представляющей интерес молекулы посредством нековалентной абсорбции на поверхности наночастицы.
3. Способ по п.1, дополнительно предусматривающий абсорбцию представляющей интерес молекулы в наночастицу.
4. Способ по п.1, дополнительно предусматривающий позволение поглощения наночастицы в компартмент клетки растения, содержащей клеточную стенку.
5. Способ по п.4, дополнительно предусматривающий покрытие наночастицы нацеливающим на субклеточный компартмент белком.
6. Способ по п.4, где компартмент выбран из группы, состоящей из цитозоля, ядра, тонопластов, пластиды, этиопласта, хромопласта, лейкопласта, элайопласта, протеинопласта, амилопласта, хлоропласта и просвета двойной мембраны.
7. Способ по п.1, где клетка растения, содержащая клеточную стенку, выбрана из группы, состоящей из клеток табака, моркови, кукурузы, канолы, рапса, хлопчатника, пальмы, арахиса, сои, Oryza sp., Arabidopsis sp., Ricinus sp. и сахарного тростника.
8. Способ по п.1, где клетка растения происходит из ткани, выбранной из группы, состоящей из зародыша, меристемы, каллуса, пыльцы, листьев, пыльников, корней, кончиков корней, цветков, семян, стручков и стеблей.
9. Способ по п.1, где наночастица выбрана из группы, состоящей из наночастиц золота, покрытых золотом наночастиц, пористых наночастиц, мезопористых наночастиц, наночастиц диоксида кремния, наночастиц полимера, вольфрамовых наночастиц, желатиновых наночастиц, нанооболочек, наносердцевин (нанокоров), наносфер, наностержней, магнитных наночастиц и их комбинацией.
10. Способ по п.1, дополнительно предусматривающий дериватизацию поверхности наночастицы.
11. Способ по п.1, где представляющая интерес молекула содержит компонент, выбранный из группы, состоящей из молекул нуклеиновых кислот, ДНК, РНК, RNAi, генов, плазмид, космид, YAC, BAC, полипептидов, ферментов, гормонов, гликопептидов, сахаров, жиров, сигнальных пептидов, антител, витаминов, мессенджеров, вторичных мессенджеров, аминокислот, цАМФ, лекарственных средств, гербицидов, фунгицидов, антибиотиков и их комбинаций.
12. Способ по п.10, где представляющая интерес молекула содержит ген.
13. Способ по п.11, где ген является геном чужеродного белка, агрономическим геном или маркерным геном.
14. Способ по п.11, дополнительно предусматривающий отбор клеток, которые стабильно интегрировали представляющую интерес молекулу.
15. Способ по п.13, где отобранные клетки являются регенерируемыми клетками.
16. Способ по п.14, дополнительно предусматривающий регенерацию растения из регенерируемых клеток.
17. Способ экспрессии гена, предусматривающий
обеспечение клетки растения, имеющей клеточную стенку;
покрытие наночастицы геном;
приведение клетки, имеющей клеточную стенку, и имеющей покрытие наночастицы в контакт друг с другом;
позволение поглощения наночастицы и представляющей интерес молекулы в клетку растения, содержащую клеточную стенку; и
экспрессию гена.
18. Способ по п.16, где ген экспрессируется в хлоропласте.
19. Способ по п.16, дополнительно предусматривающий отбор на клетки, экспрессирующие ген.
20. Способ переноса молекулярного вещества в клетку растения, предусматривающий
покрытие наночастицы плазмидной ДНК; и
контактирование имеющей покрытие наночастицы с интактными, несущими клеточную стенку клетками растения при условиях, допускающих поглощение имеющих покрытие наночастиц в клетку растения.
21. Способ по п.19, где наночастица содержит золото.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97805907P | 2007-10-05 | 2007-10-05 | |
| US60/978,059 | 2007-10-05 | ||
| PCT/US2008/078860 WO2009046384A1 (en) | 2007-10-05 | 2008-10-03 | Methods for transferring molecular substances into plant cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2010112423A true RU2010112423A (ru) | 2011-10-10 |
| RU2495935C2 RU2495935C2 (ru) | 2013-10-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010112423/10A RU2495935C2 (ru) | 2007-10-05 | 2008-10-03 | Способы переноса молекулярных веществ в клетки растений |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US8722410B2 (ru) |
| EP (1) | EP2195438B1 (ru) |
| JP (1) | JP5507459B2 (ru) |
| CN (1) | CN101889090B (ru) |
| AR (1) | AR068690A1 (ru) |
| AU (1) | AU2008308486B2 (ru) |
| BR (1) | BRPI0817911B8 (ru) |
| CA (1) | CA2701636C (ru) |
| ES (1) | ES2402341T3 (ru) |
| NZ (1) | NZ584406A (ru) |
| RU (1) | RU2495935C2 (ru) |
| WO (1) | WO2009046384A1 (ru) |
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| US8097712B2 (en) | 2007-11-07 | 2012-01-17 | Beelogics Inc. | Compositions for conferring tolerance to viral disease in social insects, and the use thereof |
| US9339539B2 (en) * | 2008-11-07 | 2016-05-17 | Hidaca Limited | Transfection with magnetic nanoparticles |
| CA2747124C (en) * | 2008-12-17 | 2018-09-04 | Dow Agrosciences Llc | Targeted integration into the zp15 locus |
| US9167835B2 (en) | 2008-12-30 | 2015-10-27 | Philip Morris Usa Inc. | Dissolvable films impregnated with encapsulated tobacco, tea, coffee, botanicals, and flavors for oral products |
| US9167847B2 (en) | 2009-03-16 | 2015-10-27 | Philip Morris Usa Inc. | Production of coated tobacco particles suitable for usage in a smokeless tobacoo product |
| HRP20150564T1 (hr) * | 2009-04-07 | 2015-08-28 | Dow Agrosciences Llc | Nanoäśesticama posredovana sekvenca specifiäśnih nukleaza |
| US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
| US8409858B2 (en) * | 2009-10-22 | 2013-04-02 | The United States Of America, As Represented By The Secretary Of The Navy | Modular functional peptides for the intracellular delivery of nanoparticles |
| BR112012012522A2 (pt) * | 2009-11-24 | 2015-09-15 | Dow Agrosciences Llc | controle de voluntárias dicotiledôneas com add em culturas monocotiledôneas |
| PH12012501491B1 (en) * | 2010-01-22 | 2019-06-28 | Dow Agrosciences Llc | Targeted genomic alteration |
| CA2788850C (en) | 2010-02-09 | 2019-06-25 | Sangamo Biosciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
| US9121022B2 (en) | 2010-03-08 | 2015-09-01 | Monsanto Technology Llc | Method for controlling herbicide-resistant plants |
| JP5922094B2 (ja) * | 2010-03-31 | 2016-05-24 | ダウ アグロサイエンシィズ エルエルシー | 生体分子の植物細胞中への送達のための植物ペプチドγ−ゼイン |
| US11236351B2 (en) | 2010-05-17 | 2022-02-01 | Dow Agrosciences Llc | Production of DHA and other LC PUFAs in plants |
| WO2012006439A2 (en) * | 2010-07-07 | 2012-01-12 | Dow Agrosciences Llc | Production of functionalized linear dna cassette and quantum dot/nanoparticle mediated delivery in plants |
| CA3065095C (en) * | 2010-07-07 | 2023-02-14 | Dow Agrosciences Llc | Method for introgressing a trait into a plant |
| AU2011336364B2 (en) | 2010-12-03 | 2017-04-13 | Corteva Agriscience Llc | Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof |
| WO2012075429A1 (en) | 2010-12-03 | 2012-06-07 | Dow Agrosciences Llc | Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof |
| JP6067671B2 (ja) * | 2011-03-23 | 2017-01-25 | ダウ アグロサイエンシィズ エルエルシー | 植物におけるイメージングおよび送達適用に適した量子ドット担体ペプチドコンジュゲート体 |
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| US20140242703A1 (en) | 2014-08-28 |
| WO2009046384A1 (en) | 2009-04-09 |
| US20170002369A1 (en) | 2017-01-05 |
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| BRPI0817911B1 (pt) | 2019-07-30 |
| BRPI0817911A2 (pt) | 2014-10-07 |
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