RU2009122029A - METHOD FOR CULTIVATION OF AMNIOTIC LIQUID CELLS AND HUMAN FIBROBLASTS IN THE SPECIALIZED AMNIOCAR ENVIRONMENT - Google Patents
METHOD FOR CULTIVATION OF AMNIOTIC LIQUID CELLS AND HUMAN FIBROBLASTS IN THE SPECIALIZED AMNIOCAR ENVIRONMENT Download PDFInfo
- Publication number
- RU2009122029A RU2009122029A RU2009122029/10A RU2009122029A RU2009122029A RU 2009122029 A RU2009122029 A RU 2009122029A RU 2009122029/10 A RU2009122029/10 A RU 2009122029/10A RU 2009122029 A RU2009122029 A RU 2009122029A RU 2009122029 A RU2009122029 A RU 2009122029A
- Authority
- RU
- Russia
- Prior art keywords
- cells
- specialized
- human fibroblasts
- amniotic fluid
- amniocar
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title claims abstract 13
- 210000002950 fibroblast Anatomy 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims abstract 5
- 239000007788 liquid Substances 0.000 title 1
- 210000004381 amniotic fluid Anatomy 0.000 claims abstract 6
- 238000012258 culturing Methods 0.000 claims abstract 4
- 230000035755 proliferation Effects 0.000 claims abstract 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 2
- 150000001413 amino acids Chemical class 0.000 claims abstract 2
- 230000010261 cell growth Effects 0.000 claims abstract 2
- 230000001413 cellular effect Effects 0.000 claims abstract 2
- 239000008103 glucose Substances 0.000 claims abstract 2
- 238000011534 incubation Methods 0.000 claims abstract 2
- 239000000463 material Substances 0.000 claims abstract 2
- 150000003839 salts Chemical class 0.000 claims abstract 2
- 239000002356 single layer Substances 0.000 claims abstract 2
- 239000000021 stimulant Substances 0.000 claims abstract 2
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Способ культивирования клеток амниотической жидкости и фибробластов человека в специализированной среде, обеспечивающей эффективное прикрепление и пролиферацию клеточного материала в составе монослоя, отличающийся тем, что инкубирование клеток проводят в вентилируемых культуральных флаконах в атмосфере 5% CO2 и выдерживают в течение 7-11 сут. ! 2. Способ культивирования клеток амниотической жидкости и фибробластов человека в специализированной среде по п.1, отличающийся тем, что полную смену среды проводят через 72 ч после начала инкубации. ! 3. Специализированная среда «Амниокар» для культивирования клеток амниотической жидкости и других клеток мезенхимального происхождения, включающая глюкозу, органические и неорганические соли, аминокислоты, отличающаяся тем, что содержит модифицированную основу и стимуляторы клеточного роста, обеспечивающие эффективное размножение пролиферативно малоактивных клеток. 1. A method of cultivating cells of amniotic fluid and human fibroblasts in a specialized environment that provides effective attachment and proliferation of cellular material in a monolayer, characterized in that the cells are incubated in ventilated culture bottles in an atmosphere of 5% CO2 and incubated for 7-11 days. ! 2. A method of culturing amniotic fluid cells and human fibroblasts in a specialized medium according to claim 1, characterized in that the complete change of the medium is carried out 72 hours after the start of incubation. ! 3. Specialized medium “Amniocar” for culturing cells of amniotic fluid and other cells of mesenchymal origin, including glucose, organic and inorganic salts, amino acids, characterized in that it contains a modified base and cell growth stimulants that ensure efficient proliferation of proliferatively inactive cells.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009122029/10A RU2415929C2 (en) | 2009-06-10 | 2009-06-10 | Specialised medium for human amniotic fluid and fibroblast cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009122029/10A RU2415929C2 (en) | 2009-06-10 | 2009-06-10 | Specialised medium for human amniotic fluid and fibroblast cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2009122029A true RU2009122029A (en) | 2010-12-27 |
| RU2415929C2 RU2415929C2 (en) | 2011-04-10 |
Family
ID=44052297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009122029/10A RU2415929C2 (en) | 2009-06-10 | 2009-06-10 | Specialised medium for human amniotic fluid and fibroblast cell culture |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2415929C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118240744A (en) * | 2024-03-06 | 2024-06-25 | 上海迈邦生物科技有限公司 | A method for simultaneously regulating cell expression product aggregates, charge heterogeneity and improving yield |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102573837B1 (en) * | 2014-06-10 | 2023-09-01 | 폴리바이오셉트 게엠베하 | Culture medium for cellular immunotherapy |
| RU2630658C1 (en) * | 2016-06-28 | 2017-09-11 | Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) | Method of cultivation of amniotic liquid cells |
| EP4419113A4 (en) * | 2021-10-20 | 2025-11-26 | Childrens Hospital Philadelphia | Physiological salt solution and methods for its preparation and use |
-
2009
- 2009-06-10 RU RU2009122029/10A patent/RU2415929C2/en not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118240744A (en) * | 2024-03-06 | 2024-06-25 | 上海迈邦生物科技有限公司 | A method for simultaneously regulating cell expression product aggregates, charge heterogeneity and improving yield |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2415929C2 (en) | 2011-04-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20120611 |