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RU2009144091A - APPLICATION OF PROTEIN HYDROLYSES FOR STABILIZATION OF METALLOPROTEASIS DETERGENT COMPOSITIONS - Google Patents

APPLICATION OF PROTEIN HYDROLYSES FOR STABILIZATION OF METALLOPROTEASIS DETERGENT COMPOSITIONS Download PDF

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RU2009144091A
RU2009144091A RU2009144091/10A RU2009144091A RU2009144091A RU 2009144091 A RU2009144091 A RU 2009144091A RU 2009144091/10 A RU2009144091/10 A RU 2009144091/10A RU 2009144091 A RU2009144091 A RU 2009144091A RU 2009144091 A RU2009144091 A RU 2009144091A
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neutral metalloprotease
protein
inhibitor
composition
composition according
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RU2484138C2 (en
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Санг-Киу ЛИ (US)
Санг-Киу ЛИ
Дебора С. ВИНЕЦКИ (US)
Дебора С. ВИНЕЦКИ
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ДАНИСКО ЮЭс ИНК., ДЖЕНЕНКОР ДИВИЖН (US)
ДАНИСКО ЮЭс ИНК., ДЖЕНЕНКОР ДИВИЖН
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8146—Metalloprotease (E.C. 3.4.24) inhibitors, e.g. tissue inhibitor of metallo proteinase, TIMP
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D11/00—Special methods for preparing compositions containing mixtures of detergents
    • C11D11/0094—Process for making liquid detergent compositions, e.g. slurries, pastes or gels
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/08—Liquid soap, e.g. for dispensers; capsuled
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1. Жидкий детергентный состав, содержащий: ! (a) от примерно 1% до примерно 75% поверхностно-активного вещества по массе; ! (b) от примерно 10% до примерно 95% воды по массе; ! (c) от примерно 0,01% до примерно 5% нейтральной металлопротеазы по массе; и ! (d) количество ингибитора нейтральной металлопротеазы, такое, что ингибитор связывается с по меньшей мере 90% молекул нейтральной металлопротеазы перед применением, причем соответствующее разбавление детергентного состава приводит к диссоциации ингибитора по меньшей мере с примерно 25% связанных молекул нейтральной металлопротеазы. ! 2. Состав по п.1, где соответствующее разбавление детергентного состава приводит к диссоциации ингибитора с по меньшей мере примерно 45% связанных молекул нейтральной металлопротеазы. ! 3. Состав по п.1, где количество ингибитора составляет от примерно 0,01% до примерно 15% по массе. ! 4. Состав по п.1, где ингибитор конкурентно ингибирует нейтральную металлопротеазу с кажущейся Ki от примерно 5 мМ до примерно 15 мМ при pH от примерно 7,5 до примерно 9,5. ! 5. Состав по п.1, где нейтральную металлопротеазу выделяют из Bacillus sp. ! 6. Состав по п.5, где нейтральная металлопротеаза представляет собой NprE. ! 7. Состав по п.1, где ингибитор представляет собой белковый гидролизат. ! 8. Состав по п.7, где белковый гидролизат выбран из гидролизата пшеничной клейковины, кислого гидролизата соевого белка, кислого гидролизата казеина коровьего молока, ферментативного гидролизата растительного белка и их комбинаций. ! 9. Состав по п.1, где ингибитор включает продукт гидролиза, полученный в результате расщепления белка под действием по меньшей мере одной нейтральной металлопротеазы. ! 10. � 1. Liquid detergent composition containing:! (a) from about 1% to about 75% surfactant by weight; ! (b) from about 10% to about 95% water by weight; ! (c) from about 0.01% to about 5% neutral metalloprotease by weight; and! (d) an amount of the neutral metalloprotease inhibitor such that the inhibitor binds to at least 90% of the neutral metalloprotease molecules prior to use, with appropriate dilution of the detergent composition causing the inhibitor to dissociate from at least about 25% of the bound neutral metalloprotease molecules. ! 2. The composition of claim 1, wherein appropriate dilution of the detergent composition results in the dissociation of the inhibitor from at least about 45% of the bound neutral metalloprotease molecules. ! 3. The composition of claim 1, wherein the amount of inhibitor is from about 0.01% to about 15% by weight. ! 4. The composition of claim 1, wherein the inhibitor competitively inhibits neutral metalloprotease with an apparent Ki of about 5 mM to about 15 mM at a pH of about 7.5 to about 9.5. ! 5. The composition of claim 1, wherein the neutral metalloprotease is isolated from Bacillus sp. ! 6. The composition of claim 5, wherein the neutral metalloprotease is NprE. ! 7. The composition of claim 1, wherein the inhibitor is a protein hydrolyzate. ! 8. The composition of claim 7, wherein the protein hydrolyzate is selected from wheat gluten hydrolyzate, soy protein acid hydrolyzate, cow's milk casein acid hydrolyzate, vegetable protein enzymatic hydrolyzate, and combinations thereof. ! 9. The composition of claim 1, wherein the inhibitor comprises a hydrolysis product resulting from the cleavage of a protein by the action of at least one neutral metalloprotease. ! 10. �

Claims (30)

1. Жидкий детергентный состав, содержащий:1. A liquid detergent composition containing: (a) от примерно 1% до примерно 75% поверхностно-активного вещества по массе;(a) from about 1% to about 75% by weight of a surfactant by weight; (b) от примерно 10% до примерно 95% воды по массе;(b) from about 10% to about 95% water by weight; (c) от примерно 0,01% до примерно 5% нейтральной металлопротеазы по массе; и(c) from about 0.01% to about 5% neutral metalloprotease by weight; and (d) количество ингибитора нейтральной металлопротеазы, такое, что ингибитор связывается с по меньшей мере 90% молекул нейтральной металлопротеазы перед применением, причем соответствующее разбавление детергентного состава приводит к диссоциации ингибитора по меньшей мере с примерно 25% связанных молекул нейтральной металлопротеазы.(d) an amount of a neutral metalloprotease inhibitor, such that the inhibitor binds to at least 90% of the neutral metalloprotease molecules before use, wherein appropriate dilution of the detergent composition will dissociate the inhibitor from at least about 25% of the associated neutral metalloprotease molecules. 2. Состав по п.1, где соответствующее разбавление детергентного состава приводит к диссоциации ингибитора с по меньшей мере примерно 45% связанных молекул нейтральной металлопротеазы.2. The composition according to claim 1, where the corresponding dilution of the detergent composition leads to the dissociation of the inhibitor with at least about 45% of the associated molecules of a neutral metalloprotease. 3. Состав по п.1, где количество ингибитора составляет от примерно 0,01% до примерно 15% по массе.3. The composition according to claim 1, where the amount of inhibitor is from about 0.01% to about 15% by weight. 4. Состав по п.1, где ингибитор конкурентно ингибирует нейтральную металлопротеазу с кажущейся Ki от примерно 5 мМ до примерно 15 мМ при pH от примерно 7,5 до примерно 9,5.4. The composition according to claim 1, where the inhibitor competitively inhibits a neutral metalloprotease with an apparent K i of from about 5 mm to about 15 mm at a pH of from about 7.5 to about 9.5. 5. Состав по п.1, где нейтральную металлопротеазу выделяют из Bacillus sp.5. The composition according to claim 1, where the neutral metalloprotease isolated from Bacillus sp. 6. Состав по п.5, где нейтральная металлопротеаза представляет собой NprE.6. The composition according to claim 5, where the neutral metalloprotease is an NprE. 7. Состав по п.1, где ингибитор представляет собой белковый гидролизат.7. The composition according to claim 1, where the inhibitor is a protein hydrolyzate. 8. Состав по п.7, где белковый гидролизат выбран из гидролизата пшеничной клейковины, кислого гидролизата соевого белка, кислого гидролизата казеина коровьего молока, ферментативного гидролизата растительного белка и их комбинаций.8. The composition according to claim 7, where the protein hydrolyzate is selected from a wheat gluten hydrolyzate, an acidic soy protein hydrolyzate, an acidic casein hydrolyzate of cow's milk, an enzymatic hydrolyzate of vegetable protein, and combinations thereof. 9. Состав по п.1, где ингибитор включает продукт гидролиза, полученный в результате расщепления белка под действием по меньшей мере одной нейтральной металлопротеазы.9. The composition according to claim 1, where the inhibitor includes a hydrolysis product obtained by protein breakdown under the action of at least one neutral metalloprotease. 10. Состав по п.9, где продукт гидролиза содержит белковые фрагменты размером менее чем примерно 5000 Да.10. The composition according to claim 9, where the hydrolysis product contains protein fragments with a size of less than about 5000 Da. 11. Состав по п.9, где белок представляет собой казеин.11. The composition according to claim 9, where the protein is casein. 12. Состав по п.1, где детергентный состав включает высокоэффективный жидкий (HDL) состав.12. The composition according to claim 1, where the detergent composition includes a high performance liquid (HDL) composition. 13. Состав по п.1, где жидкий детергентный состав дополнительно содержит от примерно 5% до примерно 10% полипропиленгликоля.13. The composition according to claim 1, where the liquid detergent composition further comprises from about 5% to about 10% polypropylene glycol. 14. Состав по п.1, где жидкий детергентный состав дополнительно содержит от примерно 0,5 мМ до примерно 5 мМ ионов кальция.14. The composition according to claim 1, where the liquid detergent composition further comprises from about 0.5 mm to about 5 mm calcium ions. 15. Состав по п.1, где жидкий детергентный состав не содержит бора.15. The composition according to claim 1, where the liquid detergent composition does not contain boron. 16. Стабилизированная ингибитором композиция нейтральной металлопротеазы, содержащая:16. An inhibitor-stabilized neutral metalloprotease composition comprising: (a) от примерно 0,001% до примерно 10% по массе нейтральной металлопротеазы; и(a) from about 0.001% to about 10% by weight of a neutral metalloprotease; and (b) конкурентный ингибитор, где конкурентный ингибитор связан с по меньшей мере примерно 90% молекул указанной нейтральной металлопротеазы.(b) a competitive inhibitor, wherein the competitive inhibitor is associated with at least about 90% of the molecules of said neutral metalloprotease. 17. Композиция по п.16, в которой конкурентный ингибитор представляет собой белковый гидролизат.17. The composition according to clause 16, in which the competitive inhibitor is a protein hydrolyzate. 18. Композиция по п.17, в которой белковый гидролизат выбран из гидролизата пшеничной клейковины, кислого гидролизата соевого белка, кислого гидролизата казеина коровьего молока, ферментативного гидролизата растительного белка и их комбинаций.18. The composition of claim 17, wherein the protein hydrolyzate is selected from a wheat gluten hydrolyzate, an acidic soy protein hydrolyzate, an acidic casein hydrolyzate of cow's milk, an enzymatic hydrolyzate of vegetable protein, and combinations thereof. 19. Композиция по п.17, в которой белковый гидролизат представляет собой продукт гидролиза, полученный в результате расщепления белка под действием по меньшей мере одной нейтральной металлопротеазы.19. The composition according to 17, in which the protein hydrolyzate is a hydrolysis product obtained by splitting the protein under the action of at least one neutral metalloprotease. 20. Композиция по п.19, в которой продукт гидролиза содержит белковые фрагменты размером менее чем примерно 5000 Да.20. The composition according to claim 19, in which the hydrolysis product contains protein fragments of a size of less than about 5000 Da. 21. Композиция по п.19, в которой белок представляет собой казеин.21. The composition according to claim 19, in which the protein is casein. 22. Композиция по п.16, в которой композиция находится в виде инкапсулированной частицы.22. The composition according to clause 16, in which the composition is in the form of an encapsulated particle. 23. Композиция по п.16, в которой ингибитор конкурентно ингибирует нейтральную металлопротеазу с кажущейся Ki от примерно 5 мМ до примерно 15 мМ при pH от примерно 7,5 до примерно 9,5.23. The composition according to clause 16, in which the inhibitor competitively inhibits a neutral metalloprotease with an apparent K i from about 5 mm to about 15 mm at a pH of from about 7.5 to about 9.5. 24. Композиция по п.16, в которой нейтральную металлопротеазу выделяют из Bacillus sp.24. The composition of claim 16, wherein the neutral metalloprotease is isolated from Bacillus sp. 25. Композиция по п.16, в которой нейтральная металлопротеаза представляет собой NprE.25. The composition according to clause 16, in which the neutral metalloprotease is an NprE. 26. Способ получения стабилизированного ингибитором жидкого детергентного состава, включающий:26. A method of producing an inhibitor-stabilized liquid detergent composition, comprising: (a) инкубацию смеси, содержащей по меньшей мере одну нейтральную металлопротеазу и белковый субстрат, в водном буфере при pH в интервале от примерно 6,5 до примерно 11 и при температуре от примерно 22°C до примерно 37°C, в процессе которой расщепление субстратного белка под действием металлопротеазы приводит к образованию продукта гидролиза;(a) incubating a mixture containing at least one neutral metalloprotease and a protein substrate in an aqueous buffer at a pH in the range of from about 6.5 to about 11 and at a temperature of from about 22 ° C to about 37 ° C, during which the cleavage substrate protein under the action of metalloprotease leads to the formation of a hydrolysis product; (b) выделение продукта гидролиза с молекулярной массой менее приблизительно 5000 Да; и(b) recovering a hydrolysis product with a molecular weight of less than about 5000 Da; and (c) объединение продукта гидролиза стадии (b) с жидким детергентным составом, содержащим от примерно 0,001% до примерно 10% по массе нейтральной металлопротеазы.(c) combining the hydrolysis product of step (b) with a liquid detergent composition containing from about 0.001% to about 10% by weight of a neutral metalloprotease. 27. Способ по п.26, где инкубационная смесь содержит от примерно 0,001% до примерно 10% нейтральной металлопротеазы и от примерно 5% до примерно 20% белкового субстрата по массе.27. The method according to p, where the incubation mixture contains from about 0.001% to about 10% neutral metalloprotease and from about 5% to about 20% protein substrate by weight. 28. Способ по п.26, где нейтральная металлопротеаза представляет собой NprE, и белковый субстрат представляет собой казеин.28. The method of claim 26, wherein the neutral metalloprotease is NprE and the protein substrate is casein. 29. Способ по п.26, где детергентный состав включает детергентный состав HDL.29. The method according to p, where the detergent composition includes a detergent composition of HDL. 30. Способ получения стабилизированного ингибитором жидкого детергентного состава, включающий объединение продукта гидролиза белка с жидким детергентным составом, содержащим от примерно 0,001% до примерно 10% нейтральной металлопротеазы по массе, где продукт гидролиза белка получают путем инкубации смеси, содержащей по меньшей мере одну нейтральную металлопротеазу и белковый субстрат, в водном буфере при pH в интервале от примерно 6,5 до примерно 11 и при температуре от примерно 22°C до примерно 37°C, в процессе которой расщепление субстратного белка под действием по меньшей мере одной металлопротеазы приводит к образованию продукта гидролиза, где продукт гидролиза с молекулярной массой менее чем приблизительно 5000 Да выделяют до объединения с нейтральной металлопротеазой. 30. A method of producing an inhibitor stabilized liquid detergent composition, comprising combining a protein hydrolysis product with a liquid detergent composition containing from about 0.001% to about 10% neutral metalloprotease by weight, where the protein hydrolysis product is obtained by incubating a mixture containing at least one neutral metalloprotease and a protein substrate, in an aqueous buffer at a pH in the range of from about 6.5 to about 11 and at a temperature of from about 22 ° C to about 37 ° C, during which the cleavage of substrate b a tree under the action of at least one metalloprotease leads to the formation of a hydrolysis product, where a hydrolysis product with a molecular weight of less than about 5000 Da is isolated before combining with a neutral metalloprotease.
RU2009144091/10A 2007-04-30 2008-04-23 Application of protein hydrolysates for stabilisation of detergent compositions of metalloprotease RU2484138C2 (en)

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US91496507P 2007-04-30 2007-04-30
US60/914,965 2007-04-30
PCT/US2008/061230 WO2008134343A1 (en) 2007-04-30 2008-04-23 Use of protein hydrolysates to stabilize metalloprotease detergent formulations

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