RU2008138372A - METHOD FOR CLEANING HUMAN RECOMBINANT FACTOR VIIa - Google Patents
METHOD FOR CLEANING HUMAN RECOMBINANT FACTOR VIIa Download PDFInfo
- Publication number
- RU2008138372A RU2008138372A RU2008138372/13A RU2008138372A RU2008138372A RU 2008138372 A RU2008138372 A RU 2008138372A RU 2008138372/13 A RU2008138372/13 A RU 2008138372/13A RU 2008138372 A RU2008138372 A RU 2008138372A RU 2008138372 A RU2008138372 A RU 2008138372A
- Authority
- RU
- Russia
- Prior art keywords
- factor viia
- monoclonal antibodies
- human recombinant
- recombinant factor
- cleaning human
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000004140 cleaning Methods 0.000 title 1
- 108010013773 recombinant FVIIa Proteins 0.000 title 1
- 108010054265 Factor VIIa Proteins 0.000 abstract 4
- 229940012414 factor viia Drugs 0.000 abstract 4
- 102000005701 Calcium-Binding Proteins Human genes 0.000 abstract 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 abstract 1
- 238000002965 ELISA Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 210000004408 hybridoma Anatomy 0.000 abstract 1
- 230000004481 post-translational protein modification Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000002594 sorbent Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Способ очистки фактора VIIa, включающий сорбцию фактора VIIa на аффинном сорбенте, содержащем фиксированные моноклональные антитела, продуцируемые клонами 4F4 и 6В8 культивируемой гибридомы, отобранными по наиболее высокой степени выхода моноклональных антител к эпитопам кальций-связывающего домена в составе фактора VIIa к этому белку, последующую элюцию фактора VIIa и контроль корректности его посттрансляционной модификации и конформации с помощью иммуноферментного анализа с применением планшет, покрытых моноклональными антителами, продуцируемыми клоном 5D9. The method of purification of factor VIIa, including sorption of factor VIIa on an affinity sorbent containing fixed monoclonal antibodies produced by clones 4F4 and 6B8 of cultured hybridoma, selected for the highest degree of yield of monoclonal antibodies to epitopes of the calcium binding domain of factor VIIa to this protein, followed by elution factor VIIa and control of the correctness of its post-translational modification and conformation using enzyme-linked immunosorbent assay using a tablet coated with monoclonal antibodies, by clone 5D9.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008138372/13A RU2008138372A (en) | 2008-09-26 | 2008-09-26 | METHOD FOR CLEANING HUMAN RECOMBINANT FACTOR VIIa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008138372/13A RU2008138372A (en) | 2008-09-26 | 2008-09-26 | METHOD FOR CLEANING HUMAN RECOMBINANT FACTOR VIIa |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2008138372A true RU2008138372A (en) | 2010-04-10 |
Family
ID=42670752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008138372/13A RU2008138372A (en) | 2008-09-26 | 2008-09-26 | METHOD FOR CLEANING HUMAN RECOMBINANT FACTOR VIIa |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2008138372A (en) |
-
2008
- 2008-09-26 RU RU2008138372/13A patent/RU2008138372A/en not_active Application Discontinuation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20101011 |