RU2008134584A - METHOD FOR ISSUING LYSINE - Google Patents
METHOD FOR ISSUING LYSINE Download PDFInfo
- Publication number
- RU2008134584A RU2008134584A RU2008134584/13A RU2008134584A RU2008134584A RU 2008134584 A RU2008134584 A RU 2008134584A RU 2008134584/13 A RU2008134584/13 A RU 2008134584/13A RU 2008134584 A RU2008134584 A RU 2008134584A RU 2008134584 A RU2008134584 A RU 2008134584A
- Authority
- RU
- Russia
- Prior art keywords
- lysine
- solution
- cation
- exchange resin
- column
- Prior art date
Links
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract 19
- 239000004472 Lysine Substances 0.000 title claims abstract 19
- 238000000034 method Methods 0.000 title claims abstract 15
- 150000001768 cations Chemical class 0.000 claims abstract 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract 6
- 238000002425 crystallisation Methods 0.000 claims abstract 6
- 230000008025 crystallization Effects 0.000 claims abstract 6
- 239000003957 anion exchange resin Substances 0.000 claims abstract 4
- 239000003729 cation exchange resin Substances 0.000 claims abstract 4
- 238000010828 elution Methods 0.000 claims abstract 4
- 238000005342 ion exchange Methods 0.000 claims abstract 4
- 238000001179 sorption measurement Methods 0.000 claims abstract 4
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract 2
- AGBXYHCHUYARJY-UHFFFAOYSA-N 2-phenylethenesulfonic acid Chemical compound OS(=O)(=O)C=CC1=CC=CC=C1 AGBXYHCHUYARJY-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000002028 Biomass Substances 0.000 claims abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000003513 alkali Substances 0.000 claims abstract 2
- 238000005349 anion exchange Methods 0.000 claims abstract 2
- 238000005119 centrifugation Methods 0.000 claims abstract 2
- 238000005352 clarification Methods 0.000 claims abstract 2
- 239000013078 crystal Substances 0.000 claims abstract 2
- 239000012531 culture fluid Substances 0.000 claims abstract 2
- 238000010612 desalination reaction Methods 0.000 claims abstract 2
- 238000001035 drying Methods 0.000 claims abstract 2
- 238000001704 evaporation Methods 0.000 claims abstract 2
- 230000008020 evaporation Effects 0.000 claims abstract 2
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims abstract 2
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims abstract 2
- 150000002500 ions Chemical class 0.000 claims abstract 2
- 238000002955 isolation Methods 0.000 claims abstract 2
- -1 lysine ion Chemical class 0.000 claims abstract 2
- 238000001471 micro-filtration Methods 0.000 claims abstract 2
- 230000007935 neutral effect Effects 0.000 claims abstract 2
- 230000003472 neutralizing effect Effects 0.000 claims abstract 2
- 238000000926 separation method Methods 0.000 claims abstract 2
- 150000003839 salts Chemical class 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 230000005587 bubbling Effects 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1. Способ ионообменного выделения лизина из культуральной жидкости, включающий отделение биомассы путем микрофильтрации, осветление нативного раствора, сорбцию лизина на сульфостирольном катионите, элюцию лизина раствором щелочи, кристаллизацию лизина, центрифугирование кристаллов и сушку, отличающийся тем, что, с целью 100% заполнения лизином полной обменной емкости катионита перед сорбцией из нативного раствора полностью удаляют конкурирующие с лизином посторонние ионы путем обессоливания, а чистый раствор, содержащий лизин в солевой форме, пропускают через колонну с анионитом в ОН--форме, получая нейтральный цвиттер-ион лизина, сорбирующийся на H+-форме катиона по механизму хемосорбции. ! 2. Способ по п.1, отличающийся тем, что для обессоливания нативного раствора используют 5 ионообменных колонн с анионитом в ОН--форме и 5 колонн с сильносшитым сульфостерольным катионитов в Н+-форме, пары которых: анионит-катионит соединены последовательно, причем отношение высоты к диаметру каждой колонны составляет 0,8, а скорость пропускаемого через систему раствора составляет 2-2,5 объема на объем всех колонн в системе. ! 3. Способ по п.1, отличающийся тем, что, с целью получения максимальной концентрации элюата для элюции используют 3-5 н (предпочтительнее 4 н) раствор NaOH, который пропускают через колонну со скоростью 0,2-0,3 объема на объем катионита в колонне. ! 4. Способ по п.1, отличающийся тем, что, с целью исключения процесса выпарки элюата перед кристаллизацией процесс нейтрализации лизина до рН 4,5-5,5 осуществляют одновременно с процессом кристаллизации, подавая с помощью барботажа в элюат газообразный хлористый водород при т1. The method of ion-exchange isolation of lysine from the culture fluid, including the separation of biomass by microfiltration, clarification of the native solution, sorption of lysine on sulfostyrene cation exchange resin, elution of lysine with an alkali solution, crystallization of lysine, centrifugation of crystals and drying, characterized in that, with the aim of 100% filling with lysine Before sorption of the full exchange capacity of cation exchanger, foreign ions competing with lysine are completely removed from the native solution by desalination, and a pure solution containing lysine in saline nth form is passed through a column with anion exchange resin in the OH - form, obtaining a neutral zwitterionic lysine ion adsorbed on the H + form of the cation by the chemisorption mechanism. ! 2. The method according to claim 1, characterized in that 5 ion-exchange columns with anion exchange resin in OH-form and 5 columns with strongly cross-linked sulfosterolone cation exchangers in the H + form, the pairs of which: anion exchange resin-cation exchange resin are connected in series, are used to desalinate the native solution the ratio of height to diameter of each column is 0.8, and the speed of the solution passed through the system is 2-2.5 volumes per volume of all columns in the system. ! 3. The method according to claim 1, characterized in that, in order to obtain the maximum concentration of the eluate for elution, 3-5 n (preferably 4 n) NaOH solution is used, which is passed through the column at a rate of 0.2-0.3 volume per volume cation exchanger in the column. ! 4. The method according to claim 1, characterized in that, in order to exclude the process of evaporation of the eluate before crystallization, the process of neutralizing lysine to a pH of 4.5-5.5 is carried out simultaneously with the crystallization process, by supplying gaseous hydrogen chloride by bubbling into the eluate at t
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008134584/10A RU2485181C2 (en) | 2008-08-27 | 2008-08-27 | Method of ion-exchange isolation of lysine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008134584/10A RU2485181C2 (en) | 2008-08-27 | 2008-08-27 | Method of ion-exchange isolation of lysine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008134584A true RU2008134584A (en) | 2010-03-10 |
| RU2485181C2 RU2485181C2 (en) | 2013-06-20 |
Family
ID=42134643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008134584/10A RU2485181C2 (en) | 2008-08-27 | 2008-08-27 | Method of ion-exchange isolation of lysine |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2485181C2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2624002C2 (en) * | 2014-06-24 | 2017-06-30 | Наталия Николаевна Кисиль | Method for obtaining lysine of pharmacopoeia condition from fodder lysine |
| CN104230732B (en) * | 2014-08-22 | 2016-08-17 | 南京工业大学 | Method for extracting L-lysine from molasses fermentation liquor |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU698980A1 (en) * | 1977-11-03 | 1979-11-25 | Ордена Ленина Институт Элементоорганических Соединений Ан Ссср | Method of preparing mixture of aminoacids |
| US4835309A (en) * | 1988-04-14 | 1989-05-30 | Eastman Kodak Company | Ion exchange recovery of L-lysine |
| SU1578196A1 (en) * | 1988-09-20 | 1990-07-15 | Всесоюзный научно-исследовательский проектно-конструкторский институт прикладной биохимии | Method of isolating lysine |
| SU1740419A1 (en) * | 1990-09-27 | 1992-06-15 | Всесоюзный научно-исследовательский проектно-конструкторский институт прикладной биохимии | Method for obtaining glutamine |
| FR2679569B1 (en) * | 1991-07-26 | 1995-05-19 | Eurolysine | PROCESS FOR SEPARATING LYSINE IN THE FORM OF AQUEOUS SOLUTIONS AND USE OF SUCH SOLUTIONS FOR ANIMAL FEEDING. |
-
2008
- 2008-08-27 RU RU2008134584/10A patent/RU2485181C2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2485181C2 (en) | 2013-06-20 |
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| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20140828 |